CN101744989B - Method for extracting schisandra chinensis, ginseng and dwarf lilyturf root and preparation thereof - Google Patents
Method for extracting schisandra chinensis, ginseng and dwarf lilyturf root and preparation thereof Download PDFInfo
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- CN101744989B CN101744989B CN200810153770.4A CN200810153770A CN101744989B CN 101744989 B CN101744989 B CN 101744989B CN 200810153770 A CN200810153770 A CN 200810153770A CN 101744989 B CN101744989 B CN 101744989B
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- extract
- ethanol
- preparation
- fructus schisandrae
- schisandrae chinensis
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Abstract
The invention relates to a traditional Chinese medicine extracting method and a pharmaceutical preparation thereof, in particular to a qi-replenishing fumai preparation treating angiocardiopathy and a preparation method thereof. The preparation is prepared from ginseng, dwarf lilyturf root and schisandra chinensis.
Description
Technical field:
The present invention relates to a kind of Traditional Chinese medicine extraction method and pharmaceutical preparation thereof, particularly qi-tonifying and pulse-restoring preparation of a kind of Cardiovarscular and preparation method thereof, said preparation is by Radix Ginseng, and Radix Ophiopogonis and Fructus Schisandrae Chinensis three taste Chinese drug preparations form.
Background technology:
Radix Ginseng is the root of Araliaceae Radix Ginseng Panax ginseng C.A.Mey..Sweet, the micro-hardship of its property of medicine, flat, return lung, spleen, heart channel; Its effect strongly invigorating primordial QI, invigorating the spleen to benefit the lung, promotes the production of body fluid, the Fructus Alpiniae Oxyphyllae of calming the nerves.Application: vigour collapse has been demonstrate,proved, lung spleen heart syndrome of deficiency of kidney-QI, the thirsty and diabete of calentura deficiency of vital energy Tianjin wound.Its chemical composition comprises multiple ginsenoside, volatile oil, aminoacid, trace element and organic acid, saccharide, vitamin etc.
Having another name called dwarf lilyturf, book band grass Radix Ophiopogonis, is Liliaceae Ophiopogon perennial evergreen herbaceous plant.Fibrous root is more sturdy, and often expand at the top of root or middle part becomes spindle shape meat fritter.Its nature and flavor with return through sweet, micro-hardship, be slightly cold, GUIXIN, lung, stomach warp.Its function with cure mainly: YIN nourishing and the production of body fluid promoting, lung moistening clears away heart-fire, for dryness of the lung dry cough, chronic consumptive disease cough, Tianjin wound is thirsty, vexed insomnia, interior-heat is quenched one's thirst, dryness of the intestine constipation, pharyngeal diphtheria.Its chemical composition comprises Radix Ophiopogonis polysaccharide and Radix Ophiopogonis total saponins etc.
Fructus Schisandrae Chinensis, is commonly called as Fructus Zanthoxyli Plansipini, sliding weight of steelyard, medicine Fructus Schisandrae Chinensis, face rattan, five Fructus Armeniacae Mumes etc., ancient medical book Cheng Ta Chi Zhu, profound and, meeting and.It is the fruit of magnoliaceae schisandra.Schisandra fruit implementation Chinese medicine function supplementing QI for promoting the production of body fluid, the nourishing kidney of astringing the lung, antidiarrheal, arresting seminal emission, calm the nerves, can control the diseases such as chronic cough dyspnea due to deficiency, the few xerostomia in Tianjin, seminal emission chronic diarrhea, forgetful insomnia.Pharmacological testing proof can regulate central nervous system's excitement and process of inhibition, promotes body metabolism, regulates gastric juice and bile secretion, and hepatitis convalescent period transaminase rising person is had to reducing effect.Peel and ripe Zhong Pihan lignanoid are the effective medicinal components of Fructus Schisandrae Chinensis, comprising multiple schizandrin.Seed is fatty, and oils and fats can soap system or machine oil.Stem and leaf and seed all can extract aromatic oil.
Qi-tonifying and pulse-restoring preparation derives from ancient prescription SHENGMAI SAN, SHENGMAI SAN source < < medicine origin > >, it is by Radix Ginseng, Radix Ophiopogonis, Fructus Schisandrae Chinensis forms, have supplementing QI and nourishing YIN, multiple arteries and veins is admittedly de-, is clinically usually used in treating that acute myocardial infarction, cardiogenic shock, toxic shock, hemorrhagic shock and coronary heart disease, endocrine disturbance etc. are sick belongs to deficiency of both QI and YIN person.
Existing qi-tonifying and pulse-restoring preparation extraction process is as follows:
Chinese patent CN200310116732.9 discloses a kind of method of preparing Shengmai for injection lyophilized injectable powder, first to Fructus Schisandrae Chinensis 60-80% alcohol reflux 2-3 time, each 60-120min, reclaim ethanol to thing alcohol taste, extractum under agitation adds ethanol, make to reach 60-80% containing alcohol amount, filter, concentrating under reduced pressure obtains extracting solution A; Again with Fructus Schisandrae Chinensis medicinal residues and Radix Ginseng after ethanol extraction, adding hydration Radix Ophiopogonis decocts, after merging aqueous extract is concentrated, then through precipitate with ethanol, filter, after reclaiming ethanol, with 10-15, doubly measure after deionized water dissolving, with macroporous adsorbent resin, process, first with deionized water, be washed till sugar-free, then be washed till without Saponin and stop with ethanol, reclaim alcohol eluen, concentrate to obtain extracting solution B; By above-mentioned Radix Ginseng, the precipitation adding distil water after Radix Ophiopogonis and Fructus Schisandrae Chinensis water extract-alcohol precipitation dissolves, and filters, dry, obtains water extraction polysaccharide crude, and polysaccharide crude adding distil water is dissolved, and crosses hollow fiber column ultrafilter, obtains extracting solution C.
Chinese patent CN200410057331.5 discloses a kind of method of preparing Shengmai injection, Radix Ginseng ethanol extraction three times, merge extractive liquid,, concentrating under reduced pressure, add water to medical material than 1: 1, upper strata oil is removed in separation, filters, concentrated, vacuum drying, obtains Radix Ginseng extract; Radix Ophiopogonis and Fructus Schisandrae Chinensis decoct with water respectively, merge extractive liquid,, and concentrating under reduced pressure, adds ethanol, places, and filters, filtrate decompression is concentrated, then adds ethanol, places, and filters, and regulates pH value, places, filter, then adjust pH, reclaim under reduced pressure, vacuum drying, obtains respectively Radix Ophiopogonis, Fructus Schisandrae Chinensis extrat.
Chinese patent CN200410021920.8 discloses a kind of preparation method of qi-tonifying and pulse-restoring preparation, and Radix Ginseng Rubra ethanol extraction reclaims ethanol, and aqueous precipitation filters, concentrated, and vacuum drying, spraying are dried or lyophilization, obtain Radix Ginseng Rubra extract; Radix Ophiopogonis, Fructus Schisandrae Chinensis water extraction, after extracting solution is concentrated, precipitate with ethanol, reclaims ethanol, and medicinal liquid is concentrated, then with vacuum drying, spraying is dry or freeze-drying is dried, and obtains Radix Ophiopogonis, Fructus Schisandrae Chinensis extrat.
Chinese patent CN96117457.9 discloses a kind of SHENGMAI ZHUSHEYE and preparation method thereof, gets Radix Ginseng Rubra and mixes with Radix Ophiopogonis, by alcohol dipping, filters, and alcohol reflux extracts; For Fructus Schisandrae Chinensis, vapor distillation, decocting boil, alcohol precipitation extracts.
Chinese patent CN 200310121126.6 discloses a kind of Shengmai for injection and preparation method thereof, and source method is oozed in the medicinal component employing of Radix Ginseng Rubra, alcohol reflux boils with decocting the extraction that combines; The medicinal component of Radix Ophiopogonis adopts alcohol reflux to boil with decocting the extraction that combines; The medicinal component of Fructus Schisandrae Chinensis adopts water distillation, alcohol reflux to boil with decocting the extraction that combines; Extraction process flow process is as follows: (1) Radix Ginseng Rubra first adopts ethanol to ooze source and extracts, and obtains Radix Ginseng Rubra extracts active ingredients liquid a; By oozing the Radix Ginseng Rubra extracting in source, use again alcohol reflux subsequently, obtain Radix Ginseng Rubra extracts active ingredients liquid b; The Radix Ginseng Rubra water boiling and extraction of again alcohol reflux being crossed, filters, and precipitate with ethanol after filtrate is concentrated, then gets supernatant and filter, filtrate and above-mentioned a, b merge, and cold preservation is got supernatant and filtered, water precipitating after filtrate is concentrated, then gets supernatant and filters, and obtains the medicinal component extracting solution of Radix Ginseng Rubra C; (2) first adopt alcohol reflux Radix Ophiopogonis, cold preservation, gets supernatant and filters, water precipitating after filtrate is concentrated, then get supernatant filtration, obtain extracts active ingredients liquid d Radix Ophiopogonis; Water boiling and extraction Radix Ophiopogonis of again alcohol reflux being crossed, filter, precipitate with ethanol after filtrate is concentrated, then get supernatant and filter, reclaim ethanol and be concentrated into thick paste shape, water precipitating, then getting supernatant filters, obtain medicinal component extracting solution e Radix Ophiopogonis: by Radix Ophiopogonis extracts active ingredients liquid d and e merging, filter, obtain medicinal component extracting solution f Radix Ophiopogonis; (3) Fructus Schisandrae Chinensis distillate adopts Fructus Schisandrae Chinensis steam distillation, collect distillate and obtain Fructus Schisandrae Chinensis distillate g: the Fructus Schisandrae Chinensis of collecting after distillate first adopts alcohol reflux, cold preservation, getting supernatant filters, filter concentrated rear water precipitating, getting supernatant filters, obtain Fructus Schisandrae Chinensis extracts active ingredients liquid h: the Fructus Schisandrae Chinensis water boiling and extraction of again alcohol reflux being crossed, filter, secondary precipitate with ethanol after filtrate is concentrated, then gets supernatant and filters, reclaim ethanol and be concentrated into thick paste shape, water precipitating, then goes supernatant to filter, and obtains the medicinal component extracting solution of Fructus Schisandrae Chinensis i; Fructus Schisandrae Chinensis extracts active ingredients liquid g, h and i are merged, filter, obtain the medicinal composition extracting solution of Fructus Schisandrae Chinensis j.
Chinese patent CN99114091.5 discloses medicine of a kind of cardiovascular diseases for the treatment of and preparation method thereof, Radix Ginseng, Radix Ophiopogonis, Fructus Schisandrae Chinensis is added to hydration and decoct; Aqueous extract or eluent are through ethanol or acetone precipitation; Macroporous resin treatment; Ion exchange resin treatment; Separation obtains the effective ingredient of extract.
Chinese patent CN200510088087.3 discloses a kind of Activating Pulse Injecting Preparation And Preparation Method, the preparation method of Radix Ginseng Rubra extract adopts conventional ethanol refluxing process to extract, the preparation method of Fructus Schisandrae Chinensis extrat is, get the water extraction 3 times that schisandra chinensis medicinal material adds 6 times of amounts, each 40min, filter, merging filtrate, be concentrated into relative density 1.10～1.15, add 95% ethanol to carry out ethanol precipitation twice, make for the first time to reach 80% containing alcohol amount, make for the second time to reach 85% containing alcohol amount, filter, merging filtrate, decompression recycling ethanol is to relative density 1.10～1.15, adding water stirs evenly, standing, filter, it is 0.4% active carbon that filtrate adds w/v, boil, keep micro-30nin that boils, slightly cold filtration, this technique is not extracted the volatile oil composition in schisandra chinensis medicinal material, the preparation method of Radix Ophiopogonis extract is: get medical material Radix Ophiopogonis, after water boiling and precipitation with ethanol, filter, add water and stir evenly after filtrate recycling ethanol, standing, filter, obtain filtrate A, the precipitation of precipitate with ethanol is dissolved in water, standing, filter, obtain liquor B, filtrate A and B are merged, add charcoal treatment, filter, this technique has been extracted the polysaccharide composition in medical material Radix Ophiopogonis.
Chinese patent CN2005100081662.7 discloses a kind of Activating Pulse Injecting Preparation And Preparation Method, by Radix Ginseng Rubra medical material section, with 5～10 times, measures 60%～90% alcohol reflux 1～5 time, each 0.5～3 hour, merge backflow, filter, decompression filtrate recycling ethanol to relative density is 1.10～1.15, gained thick paste adds water, stirs evenly standing 6～20 hours of 2～SOC, filter, filtrate charcoal treatment, filters, and obtains filtrate A for dosing, get each medical material of wheat, add 5～10 and borrow the water extraction 1～4 time of amount, each 20～90min, filter, it is 1.05～1.10 that filtrate is concentrated into relative density, add 95% ethanol to carry out ethanol precipitation twice, make for the first time to reach 80% containing alcohol amount, make for the second time to reach 85% containing alcohol amount, filter, decompression filtrate recycling ethanol to relative density is 1.10～1.15, add water, stir evenly, put 6～20 hours, filter, obtain liquor B 1, the precipitation of ethanol precipitation twice is merged, add water for injection to dissolve, standing 6～20 hours, filter, obtain liquor B 2, liquor B 1 and B2 are merged, adding w/v is the charcoal treatment of 0.1-3%, filter, obtain liquor B for dosing, schisandra chinensis medicinal material adds the water extraction 1～4 time of 4～8 times of amounts, and each 20～90min filters, merging filtrate, being concentrated into relative density is 1.10～1.15, gained thick paste adds 95% ethanol to carry out ethanol precipitation twice, make for the first time to reach 80% containing alcohol amount, make for the second time to reach 85% containing alcohol amount, filter, merging filtrate, decompression recycling ethanol to relative density is 1.10～1.15, adds water, stir evenly, standing, filter, filtrate, with after charcoal treatment, filters, and obtains liquor C for dosing, filtrate A, B, C are mixed, according to conventional method, make different preparations.
Complex manufacturing in prior art, cost is high, and the problem that extraction ratio is low is never overcome.
The present invention, through research for a long time, has overcome the shortcoming of existing extraction process, has invented a kind of effective component extraction rate high, good stability, and dosage is few, instant effect, cost is low, is easy to the long-time extracting method of preserving.
Summary of the invention:
The invention provides a kind of new Yiqi and vein recovery extract and preparation method thereof.
Yiqi and vein recovery prescription is: 1 part of Radix Ginseng, 3 parts of Radix Ophiopogonis, 1.5 parts of Fructus Schisandrae Chinensis, described part is weight portion.
Wherein Radix Ginseng is Radix Ginseng Rubra or Radix Ginseng, preferably Radix Ginseng Rubra.Fructus Schisandrae Chinensis is Radix Schisandrae Bicoloris or Fructus Schisandrae Sphenantherae, preferably Radix Schisandrae Bicoloris.
Preparation method of the present invention comprises together to be extracted two taste Chinese medicines in 1 part of Radix Ginseng, 3 parts of Radix Ophiopogonis, 1.5 part of three taste Chinese medicine of Fructus Schisandrae Chinensis, and another Chinese medicine extracts separately.
Preparation method of the present invention is by Radix Ginseng and close Radix Ophiopogonis and carry, and Fructus Schisandrae Chinensis list is carried.
Preparation method of the present invention, wherein said close to carry refer to that, by Radix Ophiopogonis, Radix Ginseng mixes.With water extraction or use alcohol extraction.Described singly carrying refers to Fructus Schisandrae Chinensis extracted separately with water extraction or with alcohol extraction.
Wherein with water extraction, refer to and be soaked in water or water heating is boiled and carried or water heating and refluxing extraction,
With alcohol extraction, refer to and use the solvent that contains ethanol or other alcohols materials with soaking, percolation, the modes such as reflux are extracted, and wherein preferred alcohol is ethanol, and ethanol can be the ethanol of 10%-100% concentration.
Preparation method of the present invention, also comprises the further water of medicinal residues after extracting for the first time or for the second time or the step of alcohol extraction.
Preparation method of the present invention, also comprises the step of the extraction product after extracting being carried out to separated and purification.
Described separation and purification comprise precipitation, filter washing, chromatography, the methods such as recrystallization.
For this reason, preparation method of the present invention preferably adopts following methods
(1) Radix Ophiopogonis, people refer and synthesize and carry, and extraction step is as follows:
(1) get Radix Ginseng, Radix Ophiopogonis, with high concentration ethanol reflux, extract, liposoluble constituent, discard, concentration of alcohol scope is preferably 80-100%, (can utilize orthogonal experiment and corresponding detection method to illustrate that these two end points liposoluble constituent content are high, and the active constituent contents such as ginsenoside and ginseng polysaccharide are low), ethanol consumption is 4-8 times; Also can utilize chloroform or dichloromethane defat;
(2) with low-concentration ethanol, extract, preferred concentration range for is 4-75%, and consumption is 4-8 times.
(3) merge extractive liquid,, extracting solution concentrates to obtain extractum; Better first with n-butanol extraction before concentrated, extract is concentrated, obtains extractum; Obtain material for closing extract;
(2) Fructus Schisandrae Chinensis list is carried, and extraction step is as follows:
(1) Fructus Schisandrae Chinensis, adds 4-10 doubly to measure the ethanol of 70-95%, reflux, extract, 2-4 time, each 1-3 hour; Preferably add 6 times of amounts, the preferred 70-75% of concentration, extracts 3 times, each 1.5 hours;
(2) better, when reflux, extract,, before reflux, extract,, can also regulate pH5-6, preferably 5.5
(3) merge extractive liquid,, filters, concentrated, obtains extractum, and obtaining material is Fructus Schisandrae Chinensis list extract;
More than closing extract and single extract merges and is extract of the present invention.
The most preferred preparation method of the present invention in an embodiment.
The present invention also provides the pharmaceutical composition being prepared into as active constituents of medicine with extract of the present invention, and pharmaceutical composition of the present invention, comprises extract, and said composition can also add medicine acceptable carrier as required.
Compositions of the present invention, is the pharmaceutical dosage forms of unit dose, and described unit dosage form refers to the unit of preparation, as every of tablet, and every capsules of capsule, every bottle of oral liquid, every bag of granule etc.
Compositions of the present invention active component wherein, its shared percentage by weight in preparation can be 0.1-99.9%, all the other are medicine acceptable carrier.
Compositions of the present invention, by being mixed with above-mentioned active component and medicine acceptable carrier to obtain.
Compositions of the present invention, its pharmaceutical dosage forms can be any pharmaceutically useful dosage form, and these dosage forms comprise: tablet, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, suck agent, granule, electuary, pill, powder, unguentum, sublimed preparation, suspensoid, powder, solution, injection, suppository, ointment, plaster, cream, spray, drop, patch.Preparation of the present invention, preferably injection, more preferably freeze-dried powder.
Compositions of the present invention, the preparation of its oral administration can contain conventional excipient, such as binding agent, filler, diluent, tablet agent, lubricant, disintegrating agent, coloring agent, flavoring agent and wetting agent, can carry out coating to tablet if desired.
Applicable filler comprises cellulose, mannitol, lactose and other similar filler.Suitable disintegrating agent comprises starch, polyvinylpyrrolidone and starch derivatives, for example sodium starch glycollate.Suitable lubricant comprises, for example magnesium stearate.The acceptable wetting agent of suitable medicine comprises sodium lauryl sulphate.
Can fill by mixing, the method that tabletting etc. are conventional is prepared solid oral composition.Repeatedly mix and can make active substance be distributed in those compositionss of a large amount of filleies of whole use.
The form of oral liquid can be for example aqueous or oily suspensions, solution, Emulsion, syrup or elixir, or can be a kind of available water before use or the composite dry products of other suitable carrier.This liquid preparation can contain conventional additive, such as suspending agent, for example sorbitol, syrup, methylcellulose, gelatin, hydroxyethyl-cellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenation edible fat, emulsifying agent, for example lecithin, anhydro sorbitol monooleate or arabic gum; Non-aqueous carrier (they can comprise edible oil), for example almond oil, fractionated coconut oil, such as oily ester, propylene glycol or the ethanol of the ester of glycerol; Antiseptic, for example para hydroxybenzene methyl ester or propyl p-hydroxybenzoate or sorbic acid, and if need, can contain conventional flavouring agent or coloring agent.
For injection, the liquid unit dosage forms of preparation contains active substance of the present invention and sterile carrier.According to carrier and concentration, this compound can be suspended or dissolve.The preparation of solution is normally by active substance being dissolved in a kind of carrier, and filter-sterilized before being packed into a kind of suitable bottle or ampoule, then seals.Adjuvant for example a kind of local anesthetic, antiseptic and buffer agent also can be dissolved in this carrier.In order to improve its stability, can be after packing bottle into, this compositions is freezing, and under vacuum, water is removed.
Compositions of the present invention, when being prepared into medicament, optionally add applicable medicine acceptable carrier, described medicine acceptable carrier is selected from: meglumine, mannitol, sorbitol, sodium pyrosulfite, sodium sulfite, sodium thiosulfate, cysteine hydrochloride, TGA, methionine, vitamin C, EDETATE SODIUM, Ethylenediaminetetraacetic Acid Calcium Salt, the alkali-metal carbonate of monovalence, acetate, phosphate or its aqueous solution, hydrochloric acid, acetic acid, sulphuric acid, phosphoric acid, aminoacid, sodium chloride, potassium chloride, sodium lactate, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivative, cellulose and derivant thereof, alginate, gelatin, polyvinylpyrrolidone, glycerol, POLYSORBATE 80, agar, calcium carbonate, calcium bicarbonate, surfactant, Polyethylene Glycol, cyclodextrin, beta-schardinger dextrin-, phospholipid material, Kaolin, Pulvis Talci, calcium stearate, magnesium stearate etc.
Compositions of the present invention is determined usage and dosage according to patient's situation in use, can take every day three times, each 1-20 agent, as: 1-20 bag or grain or sheet.
Beneficial effect of the present invention:
By extracting method of the present invention, have the following advantages: solution stability testing:
Extract by embodiment 1,2 or 3, adds meglumine; 100 ℃ are heated 1 hour, and cold preservation is spent the night, and adds mannitol, filter standardize solution; Get solution 2ml, canned transparent ampulla, sealing, solution lucifuge is placed, and at 1 day, 3 days, 10 days, 30 days range estimation clarity, the results are shown in following table.
Table 1 solution stability testing
Extracting method effective component extraction rate of the present invention is high, and cost is low, simple to operate, and finished product stability is good, and dosage is few, and curative effect is high, and instant effect is easy to long-time preservation.
(polysaccharide determination adopts: phenolsulfuric acid method, referring to Zhao Hongqiao Zhu Jiong ripple Dong, like civilian extraction and the assay kind subworld 2004 that waits seed polysaccharide Radix Ophiopogonis, 12:24-26; Deoxyschizandrin and schisandrin B content assaying method referring to: Wang Ru Zhang Yancheng builds the preferred south China national defence medical journal 2006,19 (6) of the schisandra active component extraction processes such as peak: 4-6,47)
Concrete facts mode:
Below in conjunction with embodiments of the invention, further describe flesh and blood of the present invention, this embodiment does not only limit the present invention for the present invention is described.
Embodiment 1
(1) Radix Ophiopogonis, people refer and synthesize and carry, and extraction step is as follows:
(1) getting Radix Ginseng, Radix Ophiopogonis, is 90% alcohol reflux liposoluble constituent by concentration, discards, and ethanol consumption is 6 times;
(2) by 6 times of amount concentration, be 30% ethanol extraction;
(3) n-butanol extraction, extract is concentrated, merge extractive liquid,, extracting solution concentrates to obtain extractum, obtains material for closing extract;
(2) Fructus Schisandrae Chinensis list is carried, and extraction step is as follows:
(1) Fructus Schisandrae Chinensis, adds 6 times to measure 75% ethanol, reflux, extract, 3 times, each 1.5 hours;
(2) regulate pH5.5, reflux, extract;
(3) merge extractive liquid,, filters, concentrated, obtains extractum, and obtaining material is Fructus Schisandrae Chinensis list extract;
More than closing extract and single extract merges and is extract of the present invention.
Embodiment 2
(1) Radix Ophiopogonis, people refer and synthesize and carry, and extraction step is as follows:
(1) getting Radix Ginseng, Radix Ophiopogonis, is 80% alcohol reflux liposoluble constituent by 4 times of amount concentration, discards;
(2) by 4 times of amount concentration, be 4% ethanol extraction;
(3) merge extractive liquid,, extracting solution concentrates to obtain extractum, obtains material for closing extract;
(2) Fructus Schisandrae Chinensis list is carried, and extraction step is as follows:
(1) Fructus Schisandrae Chinensis, adds 4 times to measure 70% ethanol, reflux, extract, 2 times, each 1 hour;
(2) reflux, extract;
(3) merge extractive liquid,, filters, concentrated, obtains extractum, and obtaining material is Fructus Schisandrae Chinensis list extract;
More than closing extract and single extract merges and is extract of the present invention.
Embodiment 3
(1) Radix Ophiopogonis, people refer and synthesize and carry, and extraction step is as follows:
(1) getting Radix Ginseng, Radix Ophiopogonis, is 90% alcohol reflux liposoluble constituent by concentration, discards, and ethanol consumption is 6 times;
(2) by 6 times of amount concentration, be 30% ethanol extraction;
(3) n-butanol extraction, extract is concentrated, merge extractive liquid,, extracting solution concentrates to obtain extractum, obtains material for closing extract;
(2) Fructus Schisandrae Chinensis list is carried, and extraction step is as follows:
(1) Fructus Schisandrae Chinensis, adds 10 times to measure 95% ethanol, reflux, extract, 4 times, each 3 hours;
(2) regulate pH5, reflux, extract;
(3) merge extractive liquid,, filters, concentrated, obtains extractum, and obtaining material is Fructus Schisandrae Chinensis list extract;
More than closing extract and single extract merges and is extract of the present invention.
Embodiment 4
The preparation of lyophilized formulations:
(1) by the extract of embodiment 1,2 or 3, add meglumine; 100 ℃ are heated 1 hour, and cold preservation is spent the night, and adds mannitol, filter standardize solution;
(2) activated carbon decolorizing;
(3) moisturizing;
(4) aseptic filtration;
(5) fill;
(6) lyophilizing.
Embodiment 5
The preparation of other preparations:
By the extract of embodiment 1,2,3 or 4, according to galenic pharmacy routine techniques, purchase into tablet, capsule, granule, the dosage forms such as oral liquid.
Claims (6)
1. a preparation method for Yiqi and vein recovery extract, described Yiqi and vein recovery extract is by 1 part of Radix Ginseng, and 3 parts of Radix Ophiopogonis, 1.5 parts of Fructus Schisandrae Chinensis are made, and it is characterized in that, and described preparation method comprises:
(1) Radix Ophiopogonis, people refer and synthesize and carry, and extraction step is as follows:
(1) getting Radix Ginseng, Radix Ophiopogonis, is 90% alcohol reflux liposoluble constituent by concentration, discards, and ethanol consumption is 6 times;
(2) by 6 times of amount concentration, be 30% ethanol extraction;
(3) n-butanol extraction, extract is concentrated, obtains extractum, obtains material for closing extract;
(2) Fructus Schisandrae Chinensis list is carried, and extraction step is as follows:
(1) Fructus Schisandrae Chinensis, adds 6 times to measure 75% ethanol, reflux, extract, 3 times, each 1.5 hours;
Or Fructus Schisandrae Chinensis, adds 6 times to measure 75% ethanol, regulates pH5.5, reflux, extract, 3 times, each 1.5 hours;
(2) merge extractive liquid,, filters, concentrated, obtains extractum, and obtaining material is Fructus Schisandrae Chinensis list extract;
More than closing extract and single extract merges and obtains extract.
2. the Yiqi and vein recovery extract of preparing by preparation method claimed in claim 1.
3. the pharmaceutical composition that contains extract claimed in claim 2.
4. pharmaceutical composition claimed in claim 3, is injection.
5. pharmaceutical composition claimed in claim 4, is freeze-dried powder.
6. pharmaceutical composition claimed in claim 5, adds medicine acceptable carrier if desired.
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| CN1544064A (en) * | 2003-11-19 | 2004-11-10 | 张晴龙 | Freeze-dried 'Shengmai' powder for injection and its preparing process |
| CN1552335A (en) * | 2003-12-19 | 2004-12-08 | 江苏正大天晴药业股份有限公司 | Ginseng and ophiopogon saponin drops and their preparing method |
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| CN1544064A (en) * | 2003-11-19 | 2004-11-10 | 张晴龙 | Freeze-dried 'Shengmai' powder for injection and its preparing process |
| CN1552335A (en) * | 2003-12-19 | 2004-12-08 | 江苏正大天晴药业股份有限公司 | Ginseng and ophiopogon saponin drops and their preparing method |
| CN101085191A (en) * | 2006-06-08 | 2007-12-12 | 天津天士力之骄药业有限公司 | Pulse engendendering active component extraction and its preparation |
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