CN101736430A - Method for preparing silk fibroin nano-fibre with skin-care effect - Google Patents
Method for preparing silk fibroin nano-fibre with skin-care effect Download PDFInfo
- Publication number
- CN101736430A CN101736430A CN201019063008A CN201019063008A CN101736430A CN 101736430 A CN101736430 A CN 101736430A CN 201019063008 A CN201019063008 A CN 201019063008A CN 201019063008 A CN201019063008 A CN 201019063008A CN 101736430 A CN101736430 A CN 101736430A
- Authority
- CN
- China
- Prior art keywords
- skin
- silk fibroin
- silk
- fiber
- fibroin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention relates to a method for preparing a silk fibroin nano-fibre with a skin-care effect, which comprises the following steps of: (1) degumming silkworm cocoons after removing pupas to obtain fibroins, dissolving the fibroins in CaCl2, C2H5OH and H2O, dialyzing the solution and freezing and drying the solution to obtain loose porous solid silk fibroins; (2) mixing vitamin C and sodium phosphate powder C6H6Na3O9P with the silk fibroins in a sterile de-ionized water, and blending the mixture uniformly to obtain a blend electro-spinning liquid; and (3) performing electro-spinning of the blend electro-spinning liquid to obtain the silk fibroin nano-fibre carried with the vitamin C. The method for preparing the silk fibroin nanometer fibre with the skincare effect is simple, low in cost, environmentally friendly and applicable to industrial production; and the obtained fibres can be used as ideal new concept skincare products, skin wound dressings and tissue engineering scaffolds.
Description
Technical field
The invention belongs to the preparation field of silk fibroin nano-fiber, particularly relate to a kind of preparation method with silk fibroin nano-fiber of skin-care effect.
Background technology
Fibroin albumen is to be come unstuck by silkworm, is a kind of natural protein, has excellent performance such as excellent biological compatibility, biodegradability, high ventilation, poisture-penetrability, non-immunogenicity
[1,2]Be widely used in the reparation and the regeneration of tissues such as skin, bone, cartilage, tendon, nerve trachea, blood vessel.Park etc.
[3]The silk fibroin nano-fiber film that is equipped with for the solvent electro-spinning with formic acid can promote mouse preosteoblast (MC3T3-E1) to adhere to, breed, break up, and can induce the generation of bone gla protein, promotes bone tissue reparation and regeneration.
Fibroin albumen has outstanding skin-protection and health-care effect.Fibroin albumen contains 18 seed amino acids and polypeptide composition, and wherein glycine, the third atmosphere acid and 3 kinds of simple amino acid of serine occupy 85% of Tot Prot, and are very approaching with the skin and the hair of human body, thereby becomes the popular research material of artificial skin
[4]Fibroin albumen also has good moisture preserving and heat insulating ability, can help skin to regulate moisture, and its mechanism is identical with natural moisturizing factor in the human body
[5]Studies confirm that fibroin albumen also has the effect that solar radiation is resisted in the absorption ultraviolet ray, the melanin that can effectively suppress in the skin generates
[6-8]Therefore, fibroin albumen is good skin care products base material.
Vitamin is the general name of the indispensable small molecule active material of human body, and it can not synthesize in human body, perhaps can not capacity synthesize, and must absorb from the external world.Vitamin participates in biochemical reaction widely in vivo as the auxiliary element of some important enzymes, has determined some crucial metabolic process.Many vitamins are found with skin health substantial connection, thereby usually appears in some skin-protection products as additive
[9]Vitamin C has many maintenance functions to skin, being described as " beauty treatment vitamin ", is remarkable antioxidant, can help to reduce the infringement of radical pair skin, help to reduce wrinkle and improve skin texture, and the generation that suppresses dermal melanin, the melanic effect that desalination has generated are arranged
[9]
List of references
[1]Santin?M，Motta?A，Freddi?G，Cannas?M.In?vitro?evaluation?of?the?inflammatory?potential?ofthe?silk?fibroin.J?Biomed?Mater?Res?1999；46：382-389.
[2]Horan?RL，Antle?K，Collette?AL，Wang?YZ，Huang?J，Moreau?JE，Volloch?V，Kaplan?DL，Altman?GH.In?vitro?degradation?of?silk?fibroin.Biomaterials?2005；26：3385-3393.
[3]Kim?KH，Jeong?L，Park?WH，et?al.Biological?efficacy?of?silk?fibroin?nanofiber?membranesfor?guided?bone?regeneration，J?Biotechnol，2005，120：327-339.
[4] Duan Qiaoyan, Duan Xiang, Zhang Huanxiang. the application [J] of fibroin albumen in organizational project. Chinese Tissue Engineering Study and clinical rehabilitation, 2007,11 (26): 5199-20.
[5] your tinkling of pieces of jade, the Yi Huang duckweed, Chen Ping, Strategy Software Systems Co., Ltd (SSS) is prosperous. the fibroin protein latest Progress. chemical propellant and macromolecular material .2007,5 (4): 26-29
[6]Maurer-Stroh?S，Eisenhaber?B，Eisenhaber?F.N-terminal?N-myristoylation?of?Proteins：Prediction?of?substrate?Proteins?from?amino?acid?sequence.J?Mol?Biol，2002，317：541-557.
[7]winkler?S，Kaplan?DL.Silk?produced?by?engineered?bacteria?encyclopedia?of?materials：Science?and?technology.Elsevier，Amsterdam：2001.8615-8618.
[8]park?S?J，Lee?KY，Ha?WS，et?al.Structural?changes?and?their?effect?on?mechanical?propertiesof?silk?fibroin/chitosan?blends.JAPPl?Polym?Sci，1999，74：2571-2575.
[9] Li Qingren, Wang Yuemei, Ding Xuefei. the skin-care effect of vitamin and application. household chemicals science .2007,30 (1): 16.
Summary of the invention
Technical problem to be solved by this invention provides a kind of preparation method with silk fibroin nano-fiber of skin-care effect, and this method is simple, and cost is low, and is environment friendly and pollution-free, is suitable for suitability for industrialized production; The gained fiber is a kind of desirable new ideas skin care products, skin wound dressing and tissue engineering bracket.
A kind of preparation method with silk fibroin nano-fiber of skin-care effect of the present invention comprises:
(1) will go the pupa degumming silkworm cocoons to obtain fibroin, be 1: 2: 8 CaCl with mol ratio
2, C
2H
5OH, H
2O uses distill water dialysis 3-5 days, the cellular solid filament fibroin that freeze drying obtains loosening at 60 ℃ of dissolving 1.5h;
(2) with vitamin C phosphoric ester sodium powder (C
6H
6Na
3O
9P) and above-mentioned fibroin albumen with mass ratio 1-5: 100 are mixed in the aseptic deionized water, and stirring and evenly mixing obtains blend electrostatic spinning liquid;
(3) above-mentioned electrostatic spinning liquid is obtained the ascorbic silk fibroin nano-fiber of load by electrostatic spinning.
Degumming method in the described step (1) is, with the 0.5%Na that goes the pupa silk cocoon at 100 ℃
2CO
3Boil 2-5 time each 30min in the aqueous solution.
Vitamin C phosphoric ester sodium powder (C in the described step (2)
6H
6Na
3O
9P) mass ratio with fibroin albumen is 1: 100,3: 100 or 5: 100.
Aseptic deionized water in the described step (2) is that deionized water is handled through autoclave sterilization by the generation of Alpha-pure 15 type pure water instrument again.
Electrostatic spinning parameter in the described step (3): voltage: 12-20 kilovolt; Electric field distance: 60-200 millimeter; Spinning speed, the 0.3-1.0 milliliter/hour.
The method that the present invention adopts static to spin prepares nanofiber, nanofiber is owing to provide than the bigger specific area of traditional micron order material, the ascorbic fibroin albumen composite nano fiber of load that is equipped with by pure green electro-spinning can better anthropomorphic dummy's body cell epimatrix The Nomenclature Composition and Structure of Complexes, make fibroin albumen more intimate contacting be arranged with vitamin and Skin Cell, the bionical physiological environment of the best is provided for cell growth and regeneration, thereby widened this application of two classes material aspect skin-protection and health-care greatly, both can be used as cosmetics and skincare product, for example, facial mask, the dressing and the man-made support that also can be used as skin wound use.
Beneficial effect
(1) preparation method of the present invention is simple, and cost is low, and is environment friendly and pollution-free, is suitable for suitability for industrialized production;
(2) the present invention is mainly from bionic angle, utilize the preparation method of electrostatic spinning that vitamin C and good biology performance and the skin-care effect of fibroin albumen are combined, not only bionical on the structure, and it is bionical from forming, and then reach bionical on the physiological function, for the cell growth provides best physiological environment, be a kind of desirable new ideas skin care products, skin wound dressing and tissue engineering bracket;
(3) the present invention is in whole process of preparation, do not relate to any environment and the disagreeableness material of human health to have been realized pure green electrostatic spinning, prepares the green product of pure natural;
(4) China has abundant fibroin albumen resource, and electrostatic spinning is the simplest, the method the most easily of preparation nanofiber, realizes suitability for industrialized production easily, has stronger using value.
Description of drawings
Fig. 1 is the stereoscan photograph of the ascorbic fibroin albumen electrostatic spinning nano fiber of load;
Fig. 2 is the ascorbic fibroin albumen electrostatic spinning nano fiber of a load film photo.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
(1) will go the pupa silk cocoon at 100 ℃ 0.5%Na
2CO
3Boil 2-5 time in the aqueous solution, each 30min is 1: 2: 8 CaCl with mol ratio
2, C
2H
5OH, H
2O uses distill water dialysis 3-5 days, the cellular solid filament fibroin that freeze drying obtains loosening at 60 ℃ of dissolving 1.5h;
(2) above-mentioned fibroin albumen 3.344 grams are dissolved in are stirred to dissolving fully in 9 milliliters of deionized aqua sterilisas, 0.03344 gram vitamin C phosphoric ester sodium (mass ratio of vitamin C phosphoric ester sodium and fibroin albumen is 1: 100) is dissolved in 1 milliliter of aseptic deionized water, be stirred to fully dissolving, above two kinds of good solution (silk fibroin protein solution and vitamin C phosphoric ester sodium solution) of dissolving are mixed and be stirred to evenly; Mixed solution is carried out electrostatic spinning, spinning condition: voltage, 20 kilovolts; Electric field distance, 180 millimeters; Spinning speed, obtains the composite nano-fiber membrane that average diameter is 496 ± 98 nanometers by 0.3 milliliter/hour.
Embodiment 2
(1) will go the pupa silk cocoon at 100 ℃ 0.5%Na
2CO
3Boil 2-5 time in the aqueous solution, each 30min is 1: 2: 8 CaCl with mol ratio
2, C
2H
5OH, H
2O uses distill water dialysis 3-5 days, the cellular solid filament fibroin that freeze drying obtains loosening at 60 ℃ of dissolving 1.5h;
(2) above-mentioned fibroin albumen 3.344 grams are dissolved in are stirred to dissolving fully in 9 milliliters of deionized aqua sterilisas, 0.10032 gram vitamin C phosphoric ester sodium (mass ratio of vitamin C phosphoric ester sodium and fibroin albumen is 3: 100) is dissolved in 1 milliliter of aseptic deionized water, be stirred to fully dissolving, above two kinds of good solution (silk fibroin protein solution and vitamin C phosphoric ester sodium solution) of dissolving are mixed and be stirred to evenly; Mixed solution is carried out electrostatic spinning, spinning condition: voltage, 20 kilovolts; Electric field distance, 180 millimeters; Spinning speed, obtains the composite nano-fiber membrane that average diameter is 480 ± 90 nanometers by 0.3 milliliter/hour.
Embodiment 3
(1) will go the pupa silk cocoon at 100 ℃ 0.5%Na
2CO
3Boil 2-5 time in the aqueous solution, each 30min is 1: 2: 8 CaCl with mol ratio
2, C
2H
5OH, H
2O uses distill water dialysis 3-5 days, the cellular solid filament fibroin that freeze drying obtains loosening at 60 ℃ of dissolving 1.5h;
(2) above-mentioned fibroin albumen 3.344 grams are dissolved in are stirred to dissolving fully in 9 milliliters of deionized aqua sterilisas, 0.1672 gram vitamin C phosphoric ester sodium (mass ratio of vitamin C phosphoric ester sodium and fibroin albumen is 5: 100) is dissolved in 1 milliliter of aseptic deionized water, be stirred to fully dissolving, above two kinds of good solution (silk fibroin protein solution and vitamin C phosphoric ester sodium solution) of dissolving are mixed and be stirred to evenly; Mixed solution is carried out electrostatic spinning, spinning condition: voltage, 20 kilovolts; Electric field distance, 180 millimeters; Spinning speed, obtains the composite nano-fiber membrane that average diameter is 466 ± 80 nanometers by 0.3 milliliter/hour.
Claims (4)
1. preparation method with silk fibroin nano-fiber of skin-care effect comprises:
(1) will go the pupa degumming silkworm cocoons to obtain fibroin, be 1: 2: 8 CaCl with mol ratio
2, C
2H
5OH, H
2O uses distill water dialysis 3-5 days, the cellular solid filament fibroin that freeze drying obtains loosening at 60 ℃ of dissolving 1.5h;
(2) with vitamin C phosphoric ester sodium C
6H
6Na
3O
9P powder and above-mentioned fibroin albumen are with mass ratio 1-5: 100 are mixed in the aseptic deionized water, and stirring and evenly mixing obtains blend electrostatic spinning liquid;
(3) above-mentioned electrostatic spinning liquid is obtained the ascorbic silk fibroin nano-fiber of load by electrostatic spinning.
2. a kind of preparation method with silk fibroin nano-fiber of skin-care effect according to claim 1 is characterized in that: the Degumming method in the described step (1) is, with the 0.5%Na that goes the pupa silk cocoon at 100 ℃
2CO
3Boil 2-5 time each 30min in the aqueous solution.
3. a kind of preparation method with silk fibroin nano-fiber of skin-care effect according to claim 1 is characterized in that: the vitamin C phosphoric ester sodium powder C in the described step (2)
6H
6Na
3O
9The mass ratio of P and fibroin albumen is 1: 100,3: 100 or 5: 100.
4. a kind of preparation method with silk fibroin nano-fiber of skin-care effect according to claim 1 is characterized in that: the electrostatic spinning parameter in the described step (3): voltage: 12-20 kilovolt; Electric field distance: 60-200 millimeter; Spinning speed, the 0.3-1.0 milliliter/hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010190630081A CN101736430B (en) | 2010-02-05 | 2010-02-05 | Method for preparing silk fibroin nano-fibre with skin-care effect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010190630081A CN101736430B (en) | 2010-02-05 | 2010-02-05 | Method for preparing silk fibroin nano-fibre with skin-care effect |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101736430A true CN101736430A (en) | 2010-06-16 |
| CN101736430B CN101736430B (en) | 2012-07-04 |
Family
ID=42460438
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010190630081A Expired - Fee Related CN101736430B (en) | 2010-02-05 | 2010-02-05 | Method for preparing silk fibroin nano-fibre with skin-care effect |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101736430B (en) |
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102560888A (en) * | 2012-01-17 | 2012-07-11 | 东华大学 | Silk fibroin nano-fiber film loaded with water-soluble vitamin E and preparation method for nano-fiber film |
| CN102560887A (en) * | 2012-01-17 | 2012-07-11 | 东华大学 | Silk fibroin nano-fiber film loaded with vitamins A and E and preparation method thereof |
| CN103469351A (en) * | 2013-07-18 | 2013-12-25 | 嘉兴学院 | Preparation method of vitamin C-loaded silk fibroin/hyaluronic acid composite nanofibers |
| CN103768654A (en) * | 2012-10-18 | 2014-05-07 | 上海纳米技术及应用国家工程研究中心有限公司 | Water-soluble fibroin powder and preparation method thereof |
| CN104000739A (en) * | 2014-05-19 | 2014-08-27 | 东华大学 | Preparation method for fish gelatin-silk fibroin composite nanofiber membrane carrying skin-care factor |
| KR101447256B1 (en) | 2013-10-10 | 2014-10-08 | 경북대학교 산학협력단 | Process for preparing silk fibroin nano fiber |
| CN104822875A (en) * | 2012-09-27 | 2015-08-05 | 安德烈亚斯·托伊舍尔 | Product Made of Silk |
| CN105169457A (en) * | 2015-08-26 | 2015-12-23 | 东莞市达庆医疗器械有限公司 | Cationic medical dressing and preparation method thereof |
| CN106178107A (en) * | 2016-07-01 | 2016-12-07 | 浙江大学 | A kind of silkworm silk/collagen composite support and preparation and application thereof |
| CN106178074A (en) * | 2016-07-06 | 2016-12-07 | 蓝广芊 | A kind of preparation method of porous tasar silkworm cocoon shell wound dressing |
| CN107106722A (en) * | 2014-10-17 | 2017-08-29 | 里奥纳迪诺有限公司 | Wound dressing apparatus |
| CN107964735A (en) * | 2016-06-16 | 2018-04-27 | 付妮娜 | A kind of application of compound nonwoven cloth |
| CN108283607A (en) * | 2018-01-22 | 2018-07-17 | 广西科学院 | A kind of spinning liquid, i.e. spinning nanometer facial mask and preparation method thereof |
| CN111544316A (en) * | 2020-06-23 | 2020-08-18 | 烟台新时代健康产业日化有限公司 | Fibroin nanofiber-bamboo leaf flavonoid composite material and application thereof in cosmetics |
| CN111733471A (en) * | 2020-04-23 | 2020-10-02 | 罗莱生活科技股份有限公司 | Skin care fiber capable of slowly releasing skin care ingredients and preparation method and application thereof |
| CN112515998A (en) * | 2020-12-07 | 2021-03-19 | 诺斯贝尔化妆品股份有限公司 | Beauty and skin care product adopting dry-state silk fibroin nanofiber |
| CN117264360A (en) * | 2023-09-15 | 2023-12-22 | 苏州创扬新材料科技股份有限公司 | Medical antibacterial thermoplastic elastomer and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100389744C (en) * | 2006-04-06 | 2008-05-28 | 浙江省农业科学院 | Spot eliminating facial cream of fibroin and its preparing process |
-
2010
- 2010-02-05 CN CN2010190630081A patent/CN101736430B/en not_active Expired - Fee Related
Cited By (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102560887A (en) * | 2012-01-17 | 2012-07-11 | 东华大学 | Silk fibroin nano-fiber film loaded with vitamins A and E and preparation method thereof |
| CN102560887B (en) * | 2012-01-17 | 2014-01-29 | 东华大学 | Silk fibroin nano-fiber film loaded with vitamins A and E and preparation method thereof |
| CN102560888B (en) * | 2012-01-17 | 2014-08-13 | 东华大学 | Silk fibroin nano-fiber film loaded with water-soluble vitamin E and preparation method for nano-fiber film |
| CN102560888A (en) * | 2012-01-17 | 2012-07-11 | 东华大学 | Silk fibroin nano-fiber film loaded with water-soluble vitamin E and preparation method for nano-fiber film |
| CN104822875A (en) * | 2012-09-27 | 2015-08-05 | 安德烈亚斯·托伊舍尔 | Product Made of Silk |
| CN104822875B (en) * | 2012-09-27 | 2016-10-19 | 安德烈亚斯·托伊舍尔 | silk product |
| CN103768654A (en) * | 2012-10-18 | 2014-05-07 | 上海纳米技术及应用国家工程研究中心有限公司 | Water-soluble fibroin powder and preparation method thereof |
| CN103469351B (en) * | 2013-07-18 | 2016-03-02 | 嘉兴学院 | The preparation method of load ascorbic fibroin albumen/hyaluronic acid composite nano fiber |
| CN103469351A (en) * | 2013-07-18 | 2013-12-25 | 嘉兴学院 | Preparation method of vitamin C-loaded silk fibroin/hyaluronic acid composite nanofibers |
| KR101447256B1 (en) | 2013-10-10 | 2014-10-08 | 경북대학교 산학협력단 | Process for preparing silk fibroin nano fiber |
| CN104000739A (en) * | 2014-05-19 | 2014-08-27 | 东华大学 | Preparation method for fish gelatin-silk fibroin composite nanofiber membrane carrying skin-care factor |
| CN107106722A (en) * | 2014-10-17 | 2017-08-29 | 里奥纳迪诺有限公司 | Wound dressing apparatus |
| CN105169457A (en) * | 2015-08-26 | 2015-12-23 | 东莞市达庆医疗器械有限公司 | Cationic medical dressing and preparation method thereof |
| CN105169457B (en) * | 2015-08-26 | 2018-03-23 | 东莞市达庆医疗器械有限公司 | A kind of cation medical dressing and preparation method thereof |
| CN107964735A (en) * | 2016-06-16 | 2018-04-27 | 付妮娜 | A kind of application of compound nonwoven cloth |
| CN106178107A (en) * | 2016-07-01 | 2016-12-07 | 浙江大学 | A kind of silkworm silk/collagen composite support and preparation and application thereof |
| CN106178107B (en) * | 2016-07-01 | 2019-04-30 | 浙江大学 | A kind of silk/collagen composite bracket and its preparation and application |
| CN106178074A (en) * | 2016-07-06 | 2016-12-07 | 蓝广芊 | A kind of preparation method of porous tasar silkworm cocoon shell wound dressing |
| CN108283607A (en) * | 2018-01-22 | 2018-07-17 | 广西科学院 | A kind of spinning liquid, i.e. spinning nanometer facial mask and preparation method thereof |
| CN108283607B (en) * | 2018-01-22 | 2020-11-03 | 广西科学院 | Spinning solution, instant-spinning nano mask and preparation method thereof |
| CN111733471A (en) * | 2020-04-23 | 2020-10-02 | 罗莱生活科技股份有限公司 | Skin care fiber capable of slowly releasing skin care ingredients and preparation method and application thereof |
| CN111544316A (en) * | 2020-06-23 | 2020-08-18 | 烟台新时代健康产业日化有限公司 | Fibroin nanofiber-bamboo leaf flavonoid composite material and application thereof in cosmetics |
| CN111544316B (en) * | 2020-06-23 | 2022-04-15 | 烟台新时代健康产业日化有限公司 | Fibroin nanofiber-bamboo leaf flavonoid composite material and application thereof in cosmetics |
| CN112515998A (en) * | 2020-12-07 | 2021-03-19 | 诺斯贝尔化妆品股份有限公司 | Beauty and skin care product adopting dry-state silk fibroin nanofiber |
| CN117264360A (en) * | 2023-09-15 | 2023-12-22 | 苏州创扬新材料科技股份有限公司 | Medical antibacterial thermoplastic elastomer and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101736430B (en) | 2012-07-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101736430B (en) | Method for preparing silk fibroin nano-fibre with skin-care effect | |
| Liu et al. | Silk sericin-based materials for biomedical applications | |
| Zhang et al. | Layered nanofiber sponge with an improved capacity for promoting blood coagulation and wound healing | |
| Ahsan et al. | An insight on silk protein sericin: from processing to biomedical application | |
| Dong et al. | Amorphous silk nanofiber solutions for fabricating silk-based functional materials | |
| Rising et al. | Spider silk proteins: recent advances in recombinant production, structure–function relationships and biomedical applications | |
| CN101445971A (en) | Method for preparing bionic extracellular matrix silk fibroin/chitosan composite nanometer fibre | |
| CN101502671B (en) | Method for preparing silk fibroin/ P(LLA-CL) compound nano fiber structure repair stand | |
| CN103469351B (en) | The preparation method of load ascorbic fibroin albumen/hyaluronic acid composite nano fiber | |
| Vidya et al. | Silk fibroin: a promising tool for wound healing and skin regeneration | |
| CN107802878A (en) | A kind of modified gelatin/potassium-sodium niobate compound electroactive antibacterial biological dressing and its preparation and application | |
| Naskar et al. | Nonmulberry silk proteins: multipurpose ingredient in bio-functional assembly | |
| CN102886063A (en) | Preparation and application of cellulose nanocrystals (CNCs)-reinforced collagen compound substrate | |
| CN103554520B (en) | A kind of ion induction prepares the method for tussah silk fibroin nano particle | |
| CN104013995A (en) | Oxidation chitosan graft modified porcine dermal collagen micro-nano fiber membrane and preparation method thereof | |
| CN111150881A (en) | Medical recombinant collagen spray and preparation method thereof | |
| CN107556377A (en) | Recombination human source collagen and its medical nano tunica fibrosa | |
| Sabarees et al. | Emerging trends in silk fibroin based nanofibers for impaired wound healing | |
| CN103333350A (en) | Preparation method of sericin microspheres | |
| Yang et al. | Silk fibroin hydrogels could Be therapeutic biomaterials for neurological diseases | |
| Li et al. | A comprehensive review on silk fibroin as a persuasive biomaterial for bone tissue engineering | |
| Saad et al. | A comprehensive review of recent advances in silk sericin: Extraction approaches, structure, biochemical characterization, and biomedical applications | |
| CN107693845A (en) | The bilayer that ectonexine loads HAp and VEGF respectively imitates periosteum material | |
| Reddy | Innovative and multidirectional applications of natural fibre, silk-a review | |
| Chen et al. | Biological efficacy comparison of natural tussah silk and mulberry silk nanofiber membranes for guided bone regeneration |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20100616 Assignee: Hubei t.n.x. industry limited liability company Assignor: Donghua University Contract record no.: 2014420000037 Denomination of invention: Method for preparing silk fibroin nano-fibre with skin-care effect Granted publication date: 20120704 License type: Exclusive License Record date: 20140421 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120704 Termination date: 20150205 |
|
| EXPY | Termination of patent right or utility model |