CN101736056A - Biological preparation method of chitooligosaccharide - Google Patents
Biological preparation method of chitooligosaccharide Download PDFInfo
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- CN101736056A CN101736056A CN200810179151A CN200810179151A CN101736056A CN 101736056 A CN101736056 A CN 101736056A CN 200810179151 A CN200810179151 A CN 200810179151A CN 200810179151 A CN200810179151 A CN 200810179151A CN 101736056 A CN101736056 A CN 101736056A
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- enzymolysis
- chitooligosaccharide
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Abstract
A biological preparation method of chitooligosaccharide relates to a biological preparation method of chitooligosaccharide with narrow molecular-weight distribution. The invention provides a new production method of chitooligosaccharide with narrow molecular-weight distribution so as to obtain chitooligosaccharide with narrow molecular-weight distribution from plants with polysaccharide substance. The invention uses plants as raw materials and the production process mainly comprises the steps of pretreatment, enzymolysis, inactivation, separation and screening, wherein the pretreatment step comprises the following steps: removing impurities in raw materials, cleaning, air-drying and grinding to obtain small-size particle power; the enzymolysis step comprises the following steps: adding the power and water in a reaction tank, stirring, heating and then adding enzyme to perform an enzymolysis reaction; the inactivation step comprises the step that when the enzymolysis reaction meets the demand, the temperature is quickly increased to destroy the activity of enzyme and stop the enzymolysis reaction; the solid-liquid separation step comprises the following steps: centrifuging the materials obtained by enzymolysis to remove large particles, filter-pressing and performing fine filtration to obtain clear solution; and the screening step comprises the step of performing ultrafiltration and reverse osmosis concentration to the clear solution with membrane to obtain the concentrated solution of chitooligosaccharide with narrow molecular-weight distribution.
Description
Technical field:
The present invention relates to a kind of biological preparation method of narrow molecular weight distributed Chitosan poly oligosaccharide, particularly a kind of is that raw material carries out the method that enzymolysis is produced narrow molecular weight distributed Chitosan poly oligosaccharide with the plant.
Background technology:
Chitosan poly oligosaccharide also claims chitin oligo saccharide, formal name used at school β-1, and 4-oligomerization-glucosamine is a kind of brand-new product that chitosan is obtained through special biotechnology processing, is the low molecular weight product that good water solubility, function are big, biological activity is high.It has the unexistent higher solubility of chitosan and easily by the function of many uniquenesses such as organism absorption, therefore is used in many industries.1, Medicines and Health Product production: Chitosan poly oligosaccharide is described as necessary for human body the 6th vital principle, has and three transfers (immunomodulatory, regulate pH value, regulate hormone), three and fall the function that (reducing blood-fat, hypoglycemic, hypotensive), three rows (row's cholesterol, row's heavy metal ion, toxin expelling element), three press down (anticancer, anticancer shift, inhibition carcinomycin).Chitosan poly oligosaccharide still is the activation factor of beneficial bacteria of intestinal tract (as bifidus bacillus) simultaneously.Therefore, Chitosan poly oligosaccharide is widely used in the medicines and health protection food mfg.2, makeup production: Chitosan poly oligosaccharide has metabolism and the regeneration and the fabulous moisture absorbing of preserving moisture of accelerometer chrotoplast, can stop pachylosis and aging effectively.Chitosan poly oligosaccharide also has antibacterial antidermatosis and absorbs ultraviolet function in addition, especially acne is had better curative effect.In hair care articles, add the one-tenth membrane permeability that Chitosan poly oligosaccharide can keep hair surface, moistening easy combing, and can be antistatic, dust protection, antipruritic anti-dandruff.The Chitosan poly oligosaccharide chemical stability is good, and is good with composition compound property such as emulsifier surface activity, and can increase Transdermal absorption, so Chitosan poly oligosaccharide is widely used in during makeup make.3, other industry: it is remarkable that Chitosan poly oligosaccharide suppresses the spoilage organism impact of performance, is the whole food antisepsis antistaling agent of excellent property.Chitosan poly oligosaccharide also is widely used in agricultural, because it can effectively improve fruits and vegetables output, and prevention and elimination of disease and pests, the probiotics of propagation soil and bio-bacterial manure.
Summary of the invention:
The production method that the purpose of this invention is to provide a kind of new narrow molecular weight distributed Chitosan poly oligosaccharide, enzymolysis obtains narrow molecular weight distributed Chitosan poly oligosaccharide from the plant that contains polysaccharide material, creates new production approach, widens the Application Areas of narrow molecular weight distributed Chitosan poly oligosaccharide.
To achieve these goals, the present invention adopts following technical scheme, and the present invention is raw material with the plant, production technique mainly contains pre-treatment, enzymolysis, deactivation, separation, screening step, described pre-treatment is that the foreign material of raw material are removed, and cleans up, and is ground into the small-particle powder after air-dry; Enzymolysis is that powder and water are added in people's retort, stirs, heats, and adds enzyme again and carries out enzyme digestion reaction; Deactivation is to arrive when requiring when enzyme digestion reaction, and heat temperature raising is rapidly killed the activity of enzyme, enzymolysis reaction; Solid-liquid separation is that larger particles is removed in the material centrifugation behind the enzymolysis, obtains clear liquid through press filtration, smart filter again; Screening is with film clear liquid to be carried out ultrafiltration and reverse osmosis to concentrate, and obtains the narrow molecular weight distributed Chitosan poly oligosaccharide concentrated solution.
Beneficial effect of the present invention:
The present invention has opened up narrow molecular weight distributed Chitosan poly oligosaccharide production new way, and the key of method is the control of enzymolysis, deactivation.Its operational path of selecting for use is reasonable, with the plant is raw materials for production, raw material solves easily, and is cheap, and the technology of production is simpler, control easily, the narrow molecular weight distributed Chitosan poly oligosaccharide product biologically active of producing, it can induce plant synthetic, accumulation plant protecting chemical, toxinicide, widen the Application Areas of narrow molecular weight distributed Chitosan poly oligosaccharide, biotechnology applications is had new breakthrough.
Description of drawings:
Fig. 1 is the technological process of production figure that a kind of enzymolysis process is produced narrow molecular weight distributed Chitosan poly oligosaccharide.
Embodiment:
The invention will be further described below in conjunction with accompanying drawing:
Present method is a raw material with the plant that contains polysaccharide material, and the raw material of gathering is carried out pre-treatment, foreign material are removed, after cleaning up, air-dry pulverizing, sieve powder, powder can directly be supplied with enzymolysis, it is standby also can to pack refrigeration.Powder and water are added in the retort, stirring and adjusting pH value is 3.5～5.5, be heated to 30～55 ℃, add an amount of enzyme of people, constant temperature carries out enzyme digestion reaction, Heating temperature and enzyme concentration directly influence the speed of enzyme digestion reaction, Heating temperature, enzyme concentration is directly proportional with speed of response, can select by actual needs: when enzyme digestion reaction reaches requirement, heat to deactivation more than 80 ℃ rapidly, kill the active postcooling of enzyme, enzyme digestion reaction is to use the enzyme liberating polysaccharide, the vigor of enzyme exists, and reaction is just proceeded, and polysaccharide is degraded to narrow molecular weight distributed Chitosan poly oligosaccharide, be degraded to monose again, the material of reaction is the mixture of multiple sugar, and our needs are narrow molecular weight distributed Chitosan poly oligosaccharides, and to proceed to narrow molecular weight distributed Chitosan poly oligosaccharide maximum when reaction, polysaccharide, monose more after a little while, just reach requirement, heat up rapidly and kill the vigor of enzyme, enzymolysis reaction.Material behind the enzymolysis is carried out solid-liquid separation, can carry out coarse filtration earlier, remove the material slag, on whizzer, remove macrobead again, remove small-particle, obtain clear liquid through filter press, smart filter, millipore filtration etc.Clear liquid is carried out molecular screening, select for use earlier and hold back the film of cutting 10000 molecular weight and carry out ultrafiltration, discard macromole liquid, get and see through liquid, select the film of holding back 500 molecular weight again for use, under certain pressure, concentrate, discard small molecules liquid, obtain the concentrated solution of 500～10000 molecular weight.If the production aqua product, but sterilising packaging is a finished product; If the production powder product can carry out lyophilize to concentrated solution, be packaged as finished product through crushing screening.
Claims (1)
1. the biological preparation method of a Chitosan poly oligosaccharide, it is characterized in that: be raw material with the plant, production technique mainly contains pre-treatment, enzymolysis, deactivation, separation, screening step, and described pre-treatment is that the foreign material of raw material are removed, clean up, be ground into the small-particle powder after air-dry; Enzymolysis is that powder and water are added in people's retort, stirs, heats, and adds enzyme again and carries out enzyme digestion reaction; Deactivation is to arrive when requiring when enzyme digestion reaction, and heat temperature raising is rapidly killed the activity of enzyme, enzymolysis reaction; Solid-liquid separation is that larger particles is removed in the material centrifugation behind the enzymolysis, obtains clear liquid through press filtration, smart filter again; Screening is with film clear liquid to be carried out ultrafiltration and reverse osmosis to concentrate, and obtains the narrow molecular weight distributed Chitosan poly oligosaccharide concentrated solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810179151A CN101736056A (en) | 2008-11-27 | 2008-11-27 | Biological preparation method of chitooligosaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810179151A CN101736056A (en) | 2008-11-27 | 2008-11-27 | Biological preparation method of chitooligosaccharide |
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| CN101736056A true CN101736056A (en) | 2010-06-16 |
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| CN200810179151A Pending CN101736056A (en) | 2008-11-27 | 2008-11-27 | Biological preparation method of chitooligosaccharide |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2688455C1 (en) * | 2011-09-23 | 2019-05-21 | Новозимс Биоаг А/С | Chitooligosaccharides and methods of their use for increasing crop capacity |
| CN112980905A (en) * | 2021-03-11 | 2021-06-18 | 烟台泓源生物肥料有限公司 | Preparation method of chitosan oligosaccharide |
-
2008
- 2008-11-27 CN CN200810179151A patent/CN101736056A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2688455C1 (en) * | 2011-09-23 | 2019-05-21 | Новозимс Биоаг А/С | Chitooligosaccharides and methods of their use for increasing crop capacity |
| CN112980905A (en) * | 2021-03-11 | 2021-06-18 | 烟台泓源生物肥料有限公司 | Preparation method of chitosan oligosaccharide |
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Open date: 20100616 |