CN101711582A - Composition containing American ginseng extract and probiotics and application thereof - Google Patents
Composition containing American ginseng extract and probiotics and application thereof Download PDFInfo
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- CN101711582A CN101711582A CN200910194740A CN200910194740A CN101711582A CN 101711582 A CN101711582 A CN 101711582A CN 200910194740 A CN200910194740 A CN 200910194740A CN 200910194740 A CN200910194740 A CN 200910194740A CN 101711582 A CN101711582 A CN 101711582A
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Abstract
The invention relates to a composition containing an American ginseng extract and probiotics and application thereof. The composition adopts lactobacillus plantarum, bacillus acidophilus, bifidobacterium longum, the American ginseng extract, taurine, a tea extract, oligosaccharide, vitamins, mineral matters and auxiliary materials to form a compound formula. The composition can be used as a food, a medicament or a health product, is suitable for long-time eating, is mainly used for alleviating physical fatigues, enhancing the immunity, assisting the blood fat lowering, and has auxiliary protection for chemical liver injuries.
Description
Technical field
The present invention relates to a kind of composition and application thereof that contains profitable probliotics and American ginseng extract; said composition can be used as food or medicine or health products; be suitable for long-term eating, be mainly used in enhancing immunity, auxiliary antilipemic, chemical damage is had auxiliary protection and alleviating physical fatigue.
Background technology
Immunity is meant that body resists external invasion and attack, safeguards a kind of ability of internal body environmental stability.No matter be " inferior health " which kind of reason causes, no matter be " inferior health " of the sort of performance, main, the most essential is that the human body self immunologic function descends.As long as improve autoimmune function, could be away from " inferior health ".
Along with the change of growth in the living standard and diet structure, hyperlipemia crowd increases fast, and age distribution also further shifts to an earlier date, and number young, that a middle-aged person gets hyperlipemia increases greatly.The formation of hyperlipemia needs long process, itself is also little to the harm of human body, but concurrent thereupon atherosclerotic, coronary heart disease, diabetes, hypertension, apoplexy, acute pancreatitis etc. then can the serious harm people health, even threat to life.
Chemical damage is by various toxicants, to the damage that liver causes, comprises the hepatic injury that alcoholic hepatitis, drug hepatitis, chemical substance cause as the alcohol in the food, multiple medicine and other chemical substances.The waste gas that spreads unchecked use, various vehicles of the pollution of three industrial wastes, agricultural chemicals, some household chemicals, excessive drinking, medicine etc. all cause damage to liver, and the decline of liver digestive function, function of detoxification are lost.China is hepatopathy big country, and the coastal region hepatopathy incidence of disease can be up to 20%, and annually also rises with 0.07% speed.At present, the whole world has the millions of people to cause the dirty disease of alcoholic liver because of excessive alcohol absorption.In the U.S., this has become the male sex's the fourth-largest major causes of death, and alcoholic cirrhosis accounts for 54~84% of the hard cause of disease of various livers; And at home, according to statistics, have 10~20% hepatic injury to occur among a large amount of great drinkers, thousands of routine alcoholic liver injury patients are followed up a case by regular visits to discovery in 48 months, simple fatty liver patient survival rate 70%, alcoholic hepatitis survival rate 58%, alcoholic cirrhosis survival rate 49%, cirrhosis merges alcoholic hepatitis survival rate only 35%, this sick death rate even also higher than the case fatality rate of some cancer.
When human body is under the fatigue state for a long time, can produce prematurely senile and burnout syndrome.A series of inferior health problems such as gi system, immune system and cardiovascular system can appear in burnout syndrome, with the quickening pace of modern life, and the generation cumulative year after year of burnout syndrome, and be rejuvenation trend.
Thinking at " food is given birth to the mattress evaluation guide with benefit " and health care and the mechanism of action discussion thereof of probio according to calendar year 2001 FAO/WHO expert, benefit is given birth to mattress and is helped healthy, especially to gi system, immune system and the effect of cardiovascular system unsoundness, in order to prevent sub-health state, advise taking every day probio.
Therefore, exploitation has and strengthens immunity, auxiliary antilipemic, has the food of assistant protection function, alleviating physical fatigue or medicine or health products to improving aspects such as health, prevent disease, minimizing medical expenses good economic benefit and social benefit to be arranged to chemical damage.
Summary of the invention
The object of the invention provides a kind of composition and application thereof that contains profitable probliotics and American ginseng extract, is used for alleviating physical fatigue, strengthens immunity, auxiliary antilipemic, chemical damage is had auxiliary protection.Said composition does not contain any synthetic food color, hormone, sweetener, fresh monosodium glutamate and anticorrisive agent, does not conform to any forbidden drug, safety non-toxic.
The solution that realizes the foregoing invention purpose is:
A kind of composition that contains useful living mattress and American ginseng extract is characterized in that adopting Lactobacillus plantarum, lactobacillus acidophilus, bifidobacterium longum, American ginseng extract, taurine, tea extract, compound sugar, vitamin and mineral matter, auxiliary material to form compound prescription.
Lactobacillus plantarum CGMCC NO 1258, this bacterial strain on December 6th, 2004 at China Committee for Culture Collection of Microorganisms common micro-organisms center (address: No. 13, one of Zhong Guan-cun, Haidian District, BeiJing, China city, Institute of Microorganism, Academia Sinica) preservation, preservation registration number CGMCC NO 1258.
Lactobacillus acidophilus LA11-Onlly, this bacterial strain on July 13rd, 2007 at China Committee for Culture Collection of Microorganisms common micro-organisms center (address: No. 13, one of Zhong Guan-cun, Haidian District, BeiJing, China city, Institute of Microorganism, Academia Sinica) preservation, preservation registration number CGMCC NO.2106.
Bifidobacterium longum BL88-Onlly, this bacterial strain on July 13rd, 2007 at China Committee for Culture Collection of Microorganisms common micro-organisms center (address: No. 13, one of Zhong Guan-cun, Haidian District, BeiJing, China city, Institute of Microorganism, Academia Sinica) preservation, preservation registration number CGMCC NO.2107.
The described composition that contains useful living mattress and American ginseng extract; it is characterized in that according to weight percent meter; Lactobacillus plantarum CGMCC NO 1258 amount ranges are 1~10% in the said composition; lactobacillus acidophilus LA11-Onlly amount ranges is 1~5%; bifidobacterium longum BL88-Onlly amount ranges is 1~5%; the American ginseng extract amount ranges is 3~8%; the taurine amount ranges is 5~15%; tea extract 2~5%; the compound sugar amount ranges is 0~2%; vitamin and mineral matter amount ranges are 5~8%, and all the other are the food auxiliary material.
The described composition that contains profitable probliotics and American ginseng extract, it is characterized in that Lactobacillus plantarum is Lactobacillus plantarum CGMCC NO 1258 in the said composition, lactobacillus acidophilus is LA11-Onlly CGMCCNO.2106, and bifidobacterium longum is BL88-Onlly CGMCC NO.2107.
The described composition that contains profitable probliotics and American ginseng extract is characterized in that containing in the said composition American ginseng extract, taurine and tea extract.
The described composition that contains profitable probliotics and American ginseng extract is characterized in that compound sugar in the said composition comprises one or more in FOS, xylo-oligosaccharide, oligoisomaltose and the galactooligosaccharide.
The described composition that contains profitable probliotics and American ginseng extract is characterized in that vitamin comprises vitamin(e) C sodium, Cobastab in the said composition
1, B
6, in the zinc gluconate one or more.
The described composition that contains profitable probliotics and American ginseng extract is characterized in that food auxiliary material in the said composition comprises one or more in lactose, microcrystalline cellulose, starch, maltodextrin, the dolomol.
The described composition that contains profitable probliotics and American ginseng extract at alleviating physical fatigue, strengthen immunity, auxiliary antilipemic, chemical damage had application aspect assistant protection function food or medicine or the health products.
The specific embodiment
Further specify the present invention below in conjunction with embodiment:
Embodiment: the composition that contains useful living mattress and American ginseng extract is used
By filling a prescription (weight ratio): Lactobacillus plantarum CGMCC No.12585%, lactobacillus acidophilus LA11-Onlly3%, bifidobacterium longum BL88-Onlly 2%, taurine 10%, American ginseng extract 5%, vitamin(e) C sodium 5%, tea extract 4%, xylo-oligosaccharide 2%, zinc gluconate 1%, Cobastab
10.15%, Cobastab
60.15%, lactose 29.2%, microcrystalline cellulose 15%, starch 10%, maltodextrin 8%, dolomol 0.5% are re-dubbed composition.
Above-mentioned composition shows to have through Shanghai City preventive medicine research institute experimental result and strengthens immunity, auxiliary antilipemic, chemical damage is had auxiliary protection and alleviating physical fatigue function, below is experimental result:
(1) strengthens the immunity function zoopery
Said composition human body recommended amounts is 2g/60kg every day.This tests 40 normal mouses, is divided into four groups, sets up basic, normal, high three dosage groups, is respectively 0.17,0.33,1g/kg, is equivalent to 5,10,30 times of human body recommended amounts respectively, and other establishes the distilled water control group.
Detect to feed mouse antibodies product, hemolysis plaque number, thymus index and spleen index, ConA value, DTH, NK cytoactive before and after 30 days, engulf the situation of change of % and phagocytic index, applied statistics software SPSS10.0 carries out t check and correlation analysis (table 1~7).
Table 1~7 results credit by statistics analyse as can be known, the NK cytoactive of sample 0.33g/kg and 1g/kg dosage group, engulf % and compare with control group with phagocytic index, and significant difference is all arranged; Mouse antibodies product, hemolysis plaque number, thymus index and spleen index, ConA value, the DTH of sample 1g/kg dosage group compare with control group, and significant difference is all arranged.This explanation said composition has the enhancing immunity function.
The test of table 1 serum hemolysin
*: p<0.05, compare with control group
Table 2 antibody generates and detects test
*: p<0.05, compare with control group
Table 3 thymus index, spleen index
*: p<0.05, compare with control group
The conversion test of table 4 ConA inducing mouse SPL
*: p<0.05 is compared with control group
Table 5 DTH measurement result
*: p<0.05 is compared with control group
Table 6 NK cytoactive is measured
*: p<0.05 is compared with control group
The test of table 7 macrophage phagocytic
*: p<0.05 is compared with control group
(2) auxiliary lipid-lowering function zoopery
Said composition human body recommended amounts is 2g/60kg every day.50 rats are got in this experiment, with normal diet feed raise 5 days after, be divided into five groups, every group 10, set up basic, normal, high three dosage groups, be respectively 0.17,0.33,1g/kg, be equivalent to 5,10,30 times of human body recommended amounts respectively, other establishes prevention hyperlipemia model control group and distilled water blank group.The blank group gives normal diet, and other four groups give high lipid food, feed 30 days continuously after, weigh, get the situation of change that big rathole blood is surveyed every blood lipids index, applied statistics software SPSS10.0 carries out t check and correlation analysis (table 8~11).
Table 8 result tests back blank group and compares with model control group as can be known, and weightening finish obviously descends, and significant difference is arranged, and the model establishment is described, and sample does not have obvious influence to the weight of animals.
From table 9~11 results as can be known, experiment back model control group is compared with the blank group, and cholesterol and triglycerides obviously raise, and significant difference is arranged, and the model establishment is described.Sample 0.33g/kg compares with model control group with 1g/kg dosage group, and cholesterol and triglycerides obviously descend, and significant difference is all arranged.This shows that said composition has the auxiliary antilipemic effect.
Table 8 sample is to the influence (gram) of the weight of animals
*: p<0.05 is compared with control group
Table 9 sample is to the influence (mmol/L) of animal cholesterol
*: p<0.05, compare with control group
Table 10 sample is to the influence (mmol/L) of animal triglycerides
*: p<0.05, compare with control group
Table 11 sample is to the influence (mmol/L) of animal HDL
(3) chemical damage there is the assistant protection function zoopery
Said composition human body recommended amounts is 2g/60kg every day.50 mouse are got in this experiment, are divided into five groups, and 10 every group, each dosage gives sample, and negative control group and 50% ethanol liver injury model group give equivalent distilled water, feed 30 days continuously after, claim the secondary body weight weekly, adjust to the sample amount.The 30th day each dosage group and 50% ethanol liver injury model group give 50% ethanol 0.12ml/10g.bw contamination.After the fasting 16 hours, each treated animal is got liver, makes 10% LH, measures triglycerides, MDA and reductive glutathione respectively, and other gets liver and carries out histopathologic examination.Applied statistics software SPSS10.0 carries out t check and correlation analysis (table 12~14).
Table 12 result as can be known, each dosage group body weight of sample is compared difference that there are no significant with the ethanol model group.
As can be known from Table 13, the triglycerides of normal control group, MDA and reductive glutathione are compared with the ethanol model group, and significant difference is all arranged, and the model establishment is described.Sample 0.33g/kg compares with the ethanol group with 1g/kg dosage group, and significant difference is all arranged.This shows that said composition has has the assistant protection function effect to chemical damage.
Table 12 sample is to the influence (gram) of the weight of animals
Table 13 sample is to the influence of animal triglycerides, MDA and reductive glutathione
*: p<0.05 is compared with control group
(4) alleviating physical fatigue function zoopery
Said composition human body recommended amounts is 2g/60kg every day.This tests 160 normal mouses, is divided into 16 groups, with per four groups be an experimental group, design was according to dosage fed 30 days continuously.
Table 14~19 results credit by statistics analyse as can be known, and sample does not have obvious influence to the weight of animals, obviously reduce motion back mouse blood lactic acid, blood lactic acid TG-AUC, urea content, have significant difference, and animal's liver glycogen contents content is not had obvious influence.This shows that said composition has the alleviating physical fatigue function.
Table 14 sample is to the influence (gram) of the weight of animals
Table 15 sample is to the influence of mouse swimming with a load attached to the body time
*: p<0.05, compare with control group
Table 16 sample to mouse movement after the influence (mmol/L) of blood lactic acid
*: p<0.05 is compared with control group
Table 17 sample to mouse movement after the influence of blood lactic acid TG-AUC
*: p<0.05, compare with control group
Table 18 sample to mouse movement after the influence of urea
*: p<0.05, compare with control group
Table 19 sample to mouse movement after the influence of hepatic glycogen
(5) conclusion
Above-mentioned experimental result shows that said composition has immunity, the auxiliary antilipemic of enhancing, chemical damage is had auxiliary protection and alleviating physical fatigue function.
In addition; show according to identical experiment; Lactobacillus plantarum CGMCC NO 1258 amount ranges are 1~10% in said composition; lactobacillus acidophilus LA11-Onlly amount ranges is 1~5%; bifidobacterium longum BL88-Onlly amount ranges is 1~5%; the American ginseng extract amount ranges is 3~8%; the taurine amount ranges is 5~15%; tea extract 2~5%; compound sugar (comprises FOS; xylo-oligosaccharide; in oligoisomaltose and the galactooligosaccharide one or more) amount ranges is 0~2%, and vitamin and mineral matter (comprise vitamin(e) C sodium; Cobastab
1, B
6, in the zinc gluconate one or more) amount ranges is 5~8%; all the other are under the food auxiliary material situation of (comprising lactose, microcrystalline cellulose, starch, maltodextrin, dolomol); also all have immunity, the auxiliary antilipemic of enhancing, chemical damage is had auxiliary protection and alleviating physical fatigue function, and can be used for making corresponding food or medicine or health products.
Claims (8)
1. a composition that contains profitable probliotics and American ginseng extract is characterized in that adopting Lactobacillus plantarum, lactobacillus acidophilus, bifidobacterium longum, American ginseng extract, taurine, tea extract, compound sugar, vitamin and mineral matter, auxiliary material to form compound prescription.
2. the composition that contains profitable probliotics and American ginseng extract according to claim 1; it is characterized in that according to weight percent meter; Lactobacillus plantarum CGMCC NO 1258 amount ranges are 1~10% in the said composition; lactobacillus acidophilus LA11-Onlly amount ranges is 1~5%; bifidobacterium longum BL88-Onlly amount ranges is 1~5%; the American ginseng extract amount ranges is 3~8%; the taurine amount ranges is 5~15%; tea extract 2~5%; the compound sugar amount ranges is 0~2%; vitamin and mineral matter amount ranges are 5~8%, and all the other are the food auxiliary material.
3. the composition that contains profitable probliotics and American ginseng extract according to claim 1 and 2, it is characterized in that Lactobacillus plantarum is Lactobacillus plantarum CGMCC NO 1258 in the said composition, lactobacillus acidophilus is LA11-Onlly CGMCC NO.2106, and bifidobacterium longum is BL88-Onlly CGMCC NO.2107.
4. the composition that contains profitable probliotics and American ginseng extract according to claim 1 and 2 is characterized in that containing in the said composition American ginseng extract, taurine and tea extract.
5. the composition that contains profitable probliotics and American ginseng extract according to claim 1 and 2 is characterized in that compound sugar in the said composition comprises one or more in FOS, xylo-oligosaccharide, oligoisomaltose and the galactooligosaccharide.
6. the composition that contains profitable probliotics and American ginseng extract according to claim 1 and 2 is characterized in that vitamin comprises vitamin(e) C sodium, Cobastab in the said composition
1, B
6, in the zinc gluconate one or more.
7. the composition that contains profitable probliotics and American ginseng extract according to claim 1 and 2 is characterized in that food auxiliary material in the said composition comprises one or more in lactose, microcrystalline cellulose, starch, maltodextrin, the dolomol.
According to the composition that contains profitable probliotics and American ginseng extract described in claim 1 or 2 or 3 or 4 or 5 or 6 or 7 at alleviating physical fatigue, strengthen immunity, auxiliary antilipemic, chemical damage had application aspect assistant protection function food or medicine or the health products.
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