CN101675742A - Production method and applications of biological pesticide containing stachyose - Google Patents
Production method and applications of biological pesticide containing stachyose Download PDFInfo
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- CN101675742A CN101675742A CN200810211973A CN200810211973A CN101675742A CN 101675742 A CN101675742 A CN 101675742A CN 200810211973 A CN200810211973 A CN 200810211973A CN 200810211973 A CN200810211973 A CN 200810211973A CN 101675742 A CN101675742 A CN 101675742A
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Abstract
The invention provides a production method and applications of a biological pesticide containing stachyose. The biological pesticide is a solid containing more than 75% of stachyose, which is extracted from Chinese artichoke, stachysfloridona, kobold, herba lycopi or rehmanniae. The method mainly utilizes a pure water leaching technique, a high-speed physical separation technique and an ion exchange desalinization and decoloring technique. By utilizing the obvious induced disease resistance function of the stachyose on crops, the prepared biological preparation has the advantages of favorablewater-solubility, no toxicity or harm and no environmental pollution, is a natural environmental-protection biological pesticide, and has favorable effects on preventing and treating yellow rust, powdery mildew, banded sclerotial blight, gibberellic disease and anthracnose of wheat.
Description
Technical field
The present invention relates to a kind of production method and application thereof that contains the biopesticide of stachyose, key is to be to have extracted from natural plants the biopesticide that contains high purity stachyose, this biopesticide can induce crops to produce disease resistance effectively, be used to prevent and treat stripe rust of wheat, head blight, powdery mildew, banded sclerotial blight, anthracnose etc.
Background technology
Control of plant disease has two kinds of main paties: the one, and adopt chemical bactericide to kill or weaken infecting of pathogeny thing at the pathogeny bacterium; The 2nd, strengthen the disease resistance of plant itself.At present, because the extensive use of chemical bactericide, made China become one of the most serious country of pesticide pollution in the world, and then made food middle peasant medicine recall rate high, national healthy of harm.Therefore, research and development green agriculture biological products are active demands of guaranteeing China's people's health and economic healthy and rapid development.Since 20 beginning of the century Bernard find INDUCED DISEASE RESISTANCE OF PLANT, studies show that various physics, chemistry can both inducing plant produce disease resistance with biotic factor, thereby improve the disease resistance of plant, and the disease resistance that strengthens plant self to be controlling plant diseases the most basic valid approach.The biopesticide that contains stachyose be from natural plants, extract based on stachyose, other contains the novel agrochemical of raffinose, verbascose etc.Stachyose is a kind of tetrose, is made up of fructose-Glucose-Galactose-galactose, and main being used as bred the intestinal beneficial bacterium group, improves the gut flora balance, strengthens body immunity.By studies show that in a large number, utilize the stachyose solution of certain purity and concentration to spray and to improve the resistance of wheat seedling stage yellow rust in wheat, find simultaneously on other crops, to use and also show, certain purity finite concentration stachyose solution sprays the disease resistance that can significantly improve crops in crops, can be used for preventing and treating stripe rust of wheat, head blight, powdery mildew, banded sclerotial blight, anthracnose etc.The biopesticide with inducing anti-disease of high purity stachyose that utilization is extracted from natural plants preparation is compared with chemical bactericide, has significant advantage: (1), efficient, single-minded to pest, mechanism of action uniqueness; (2) to people, animal, natural enemy, beneficial organism, non-target organism safety; (3) be that C, H, O element are formed, in environment, easily decompose, free from environmental pollution; (4) development and utilization measure is many, and is easy to use.Therefore, this biopesticide meets the requirement of agricultural sustainable development strategy.
Summary of the invention
The purpose of this invention is to provide a kind of production method and application method thereof that contains the biopesticide of stachyose.With the biopesticide control corps diseases that contains stachyose, key is to have utilized stachyose can effectively induce crops self to produce the mechanism of disease resistance, thereby improves crops autoimmunity.This agricultural chemicals is taken from nature, is used for nature, meets the requirement that keeps biobalance fully.Simultaneously, expanded the frontier that stachyose is used, for a brand-new approach has been opened up in the control of corps diseases.
The biopesticide that contains stachyose of the present invention is to extract refining forming from Chinese artichoke, silver bar, kobold, Herba Lycopi or glutinous rehmannia, and wherein stachyose accounts for more than 75% of solid.Detection shows that these goods are based on stachyose, also contains raffinose, verbascose (five glycan) and is higher than the product of five glycan, and it is few to contain single, double sugar.According to the different compound methods and the needs of product form, biopesticide of the present invention can directly be prepared aborning, also can prepare with solid powder or syrup, but the content of its stachyose in solid is required to be more than 75%.
The invention provides from natural plants (Chinese artichoke, silver bar, kobold, Herba Lycopi or glutinous rehmannia) production method of extracting the biopesticide that contains stachyose, comprising following steps:
＜1 〉, the prepared using electrical conductivity is extracted less than 100 μ s/cm deionized waters and is squeezed the juice;
＜2 〉, the above-mentioned juice of prefiltration, and carry out high-temperature instantaneous sterilization, sterilization temperature 115-130 ℃, sterilizing time is 4-6 second;
＜3 〉, the juice after the sterilization adopts horizontal spiral seperator and high-speed centrifuge to separate respectively;
＜4〉liquid after the separation is handled by decolouring desalination resin, and the liquid glucose after the processing is the water white transparency liquid glucose, and sugar concentration is 5-10%, and the content of stachyose in solid content is greater than 75%;
＜5 〉, the liquid glucose after the desalination of will decolouring directly is mixed with agricultural chemicals, or is condensed into and contains the syrup of solid content more than 70%, or be spray dried to pressed powder again, obtains containing the biopesticide of stachyose.
It is the stem tuber of plant all that the present invention produces used natural plants, can adopt the directly squeezing of bright material in the harvest season during production, cleans up earlier before the squeezing, needs after the squeezing will expect slag rehydration squeezing 1-2 time with deionized water.And between the harvests, can adopt siccative production, adding 4-6 times of deionized water of raw material weight during production soaks, wait material to restore after, squeeze again, need after the squeezing will expect that with deionized water the slag rehydration squeezes 1-2 time.Concrete rehydration squeezing number of times is controlled according to the residual sugar amount, and the siccative soak time changes with the difference of producing season to some extent according to the difference of raw material.
Above-mentioned syrup is sterilized with high-temperature instantaneous sterilization technology through after filtering again, and has partly precipitated after the sterilization and produces, and precipitation utilizes horizontal spiral seperator and high-speed centrifuge combination to separate.
Liquid glucose after the separation is with the desalination of decolouring of decolouring desalination resin, the pattern of the preferred strong acid sun of decolouring desalination resin-macroporous absorbent resin-strong base negative resin, also can adopt the pattern of strong acid sun resin-macropore weak base negative resin-strong acid sun resin-strong base negative resin, also can adopt the resin dedicated pattern that adds the desalination resin of decolouring.
Liquid glucose after the decolouring desalination can directly be prepared application by the application method of this biopesticide, also can concentrate by vacuum concentrating apparatus, is condensed into to contain the syrup of solid content more than 70%, or makes powdery product by atomized drying again.
The application method that contains the biopesticide of stachyose of the present invention comprises:
＜1 〉, the satisfactory biopesticide that contains stachyose is mixed with the aqueous solution that contains stachyose 0.01%-0.1%.
＜2 〉, the satisfactory biopesticide that contains stachyose is mixed with the aqueous solution that contains stachyose 0.01%-0.1%, add 0.1% polysorbas20 simultaneously.
＜3 〉, the satisfactory biopesticide that contains stachyose is mixed with the aqueous solution that contains stachyose 0.01%-0.1%, add 0.1% polysorbas20 simultaneously, add trace element and other foliage fertilizer again.
＜4 〉, the satisfactory biopesticide that contains stachyose is mixed with the aqueous solution that contains stachyose 0.01%-0.1%, add other agricultural chemicals simultaneously, be mixed with and have the novel pesticide that has the inducing anti-disease function concurrently.
Above application method, wherein＜1 〉,＜2〉seed dressing when made biopesticide can be used for proportion of crop planting or be used for spraying of blade face in seedling stage;＜3 〉,＜4〉biopesticide of being prepared can be used for the crops foliage-spray in seedling stage.
Embodiment
Below pass through case introduction embodiment of the present invention, but do not limit practical range of the present invention with this.
Implement one
Weigh 30 kilograms of fresh herbvore silkworms, clean with flushing with clean water, squeeze behind the broken granulating 12 kilograms in juice, add 36 kilograms of deionized waters in the slag after the squeezing at twice altogether, again through squeezing, squeezing altogether 49 kilograms of syrups, pol is 8.Syrup is filtered, filter big impurity, through 120 ℃ high-temperature instantaneous sterilization device, sterilizing time is 5 seconds again.Then separate with supercentrifuge by horizontal spiral centrifuge successively, the clear liquid that centrifugation obtains is more successively by strong acid cation resin 001, resin and macroporous weak D301, strong acid cation resin 001, strong base anion resins 201, obtains pol and be 58 kilograms of 5 liquid glucoses.In the gained liquid glucose, add 4000kg water, fully after the dissolving, add soil temperature 20 (Tween-20) 4kg, mix.
(1) be used for the inducing wheat stripe rust resisting, grow to a leaf in order to the biopesticide of last method preparation at wheat seedling and spray in the blade face wholeheartedly the time, spray area 200-400 mu, but the inducing wheat stripe rust resisting, and effect is obvious, lures anti-effect to reach more than 80%.
(2) be used for the powdery mildew of apple tree and the inducing anti-disease aspect of anthracnose, the biopesticide of preparing with said method sprays the blade face in spring when apple tree germinates long tender leaf, induces effect more than 80%, and is respond well.
(3) above biopesticide also can be used for the control of crops banded sclerotial blight, wheat scab, and inducing anti-disease is respond well.
Implement two
Weigh and dry 50 kilograms of silver bars, after flushing with clean water, add 200 kilograms of deionized waters, electrical conductivity is less than 100 μ s/cm, and soak at room temperature 8 hours squeezes again.Add squeezing again after the deionized water rehydration in the slag again, mix syrup, altogether 290 kilograms of syrups, pol is 9.Syrup is filtered, filter big impurity, through 120 ℃ high-temperature instantaneous sterilization device, sterilizing time is 5 seconds.Then separate with supercentrifuge by horizontal spiral centrifuge successively, the clear liquid that centrifugation obtains is more successively by strong acid cation resin 001, macroporous absorbent resin DA201, strong base anion resins 201, obtains pol and be 320 kilograms of 6 liquid glucoses.Vacuum concentration to pol is to carry out atomized drying at 40 o'clock again, 17.88 kilograms of Icing Sugar.
The Icing Sugar of gained contains stachyose 76.68% after testing, and the method for its preparation agricultural chemicals is prepared by the application method of the biopesticide that contains stachyose and used.
The invention provides a kind of production method and application method thereof that contains the stachyose biopesticide, for high purity stachyose application agriculturally provides a brand-new application, utilize the good result of high purity stachyose aspect plant inducer adpedance characteristic of disease, be mixed with a kind of novel biopesticide with inducing anti-disease effect.This pesticide producing raw material is easy to get, production method is simple, and production cost is low, is easy to industrialization.This biopesticide is nontoxic, meets environmental protection policy, and alternative chemical bactericide helps the healthy sustainable development of agricultural, can realize " three win " of society, economy and environmental benefit, should vigorously promote the use.
Claims (10)
1, a kind of biopesticide that contains stachyose is characterized in that it being the agricultural chemicals that contains stachyose that extracts from natural plants.
2, natural plants according to claim 1 means Chinese artichoke, silver bar, kobold, Herba Lycopi or glutinous rehmannia.
3, biopesticide according to claim 1, comprising syrup, pressed powder formulation, or the agricultural chemicals that directly is mixed with.
4, mean that according to claim 1,3 described biopesticides containing stachyose in the solid content is product more than 75%.
5, a kind of production method that contains the biopesticide of stachyose, its production method comprises:
＜1 〉, the prepared using electrical conductivity is extracted less than 100 μ s/cm deionized waters and is squeezed the juice;
＜2 〉, the above-mentioned juice of prefiltration, and carry out high-temperature instantaneous sterilization, sterilization temperature 115-130 ℃, sterilizing time is 4-6 second;
＜3 〉, the juice after the sterilization adopts horizontal spiral seperator and high-speed centrifuge to separate respectively;
＜4〉liquid after the separation is handled by decolouring desalination resin, and the liquid glucose after the processing is the water white transparency liquid glucose, and sugar concentration is 5-10%, and the content of stachyose in solid content is greater than 75%;
＜5 〉, the liquid glucose after the liquid glucose after the desalination of will decolouring directly is mixed with agricultural chemicals, or is condensed into and contains the syrup of solid content more than 70%, or be spray dried to pressed powder again, obtains containing the biopesticide of stachyose.
6, the production method that contains the biopesticide of stachyose as claimed in claim 5, employed raw material can be bright material, squeezing through cleaning after, and with deionized water again rehydration squeeze; Also siccative soaks with 4-6 times of deionized water of raw material weight and to restore the back squeezing.
7, the production method that contains the biopesticide of stachyose as claimed in claim 5, wherein employed decolouring desalination resin comprises styrene gel resin, macroporous absorbent resin, decolouring is resin dedicated or macroporous ion exchange resin.
8, the biopesticide as claim 5 comprises at the application method aspect the crops inducing anti-disease:
＜1 〉, the satisfactory biopesticide that contains stachyose is mixed with the aqueous solution that contains stachyose 0.01%-0.1%.
＜2 〉, the satisfactory biopesticide that contains stachyose is mixed with the aqueous solution that contains stachyose 0.01%-0.1%, add 0.1% polysorbas20 simultaneously.
＜3 〉, the satisfactory biopesticide that contains stachyose is mixed with the aqueous solution that contains stachyose 0.01%-0.1%, add 0.1% polysorbas20 simultaneously, add trace element and other foliage fertilizer again.
＜4 〉, the satisfactory biopesticide that contains stachyose is mixed with the aqueous solution that contains stachyose 0.01%-0.1%, add other agricultural chemicals simultaneously, be mixed with and have the novel pesticide that has the inducing anti-disease function concurrently.
9, application method as claimed in claim 8, wherein＜1 〉,＜2〉seed dressing when made biopesticide can be used for proportion of crop planting or be used for spraying of blade face in seedling stage;＜3 〉,＜4〉biopesticide of being prepared can be used for the crops foliage-spray in seedling stage.
10, the biopesticide of claim 5,8 described methods productions can be used for preventing and treating stripe rust of wheat, head blight, powdery mildew, banded sclerotial blight, anthracnose etc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810211973A CN101675742A (en) | 2008-09-16 | 2008-09-16 | Production method and applications of biological pesticide containing stachyose |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810211973A CN101675742A (en) | 2008-09-16 | 2008-09-16 | Production method and applications of biological pesticide containing stachyose |
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| CN101675742A true CN101675742A (en) | 2010-03-24 |
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| CN200810211973A Pending CN101675742A (en) | 2008-09-16 | 2008-09-16 | Production method and applications of biological pesticide containing stachyose |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107079941A (en) * | 2017-06-08 | 2017-08-22 | 合肥龙滨化工科技有限公司 | A kind of botanical pesticide for preventing and treating muskmelon downy mildew |
| CN112739207A (en) * | 2018-09-28 | 2021-04-30 | 株式会社大塚制药工场 | Liquid for preserving mammalian cells comprising an acarbose or stachyose |
-
2008
- 2008-09-16 CN CN200810211973A patent/CN101675742A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107079941A (en) * | 2017-06-08 | 2017-08-22 | 合肥龙滨化工科技有限公司 | A kind of botanical pesticide for preventing and treating muskmelon downy mildew |
| CN112739207A (en) * | 2018-09-28 | 2021-04-30 | 株式会社大塚制药工场 | Liquid for preserving mammalian cells comprising an acarbose or stachyose |
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Application publication date: 20100324 |