CN101669637B - Application of cornflower-3-O-galactoside in preparing foods or medicaments for improving cognitive functions of old people - Google Patents
Application of cornflower-3-O-galactoside in preparing foods or medicaments for improving cognitive functions of old people Download PDFInfo
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- CN101669637B CN101669637B CN2009100704992A CN200910070499A CN101669637B CN 101669637 B CN101669637 B CN 101669637B CN 2009100704992 A CN2009100704992 A CN 2009100704992A CN 200910070499 A CN200910070499 A CN 200910070499A CN 101669637 B CN101669637 B CN 101669637B
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Abstract
The invention discloses an application of cornflower-3-O-galactoside in preparing foods or medicines for improving cognitive functions of old people. Results from animal experiments show that: compared with a control group (mice are fed with distilled water), the step-through latencies of animals fed with the cornflower-3-O-galactoside are all evidently prolonged; the escape latencies of mice in Morris water maze are evidently shortened; the duration time on a platform quadrant is evidently prolonged, and times of crossing the platform are evidently increased. The hint shows that the spatial memory ability of the old-aged mice compensated with the cornflower-3-O-galactoside can be evidently improved. Compared with an H2O2 model group, the LDH activity of hippocampal nerve cell supernate in the group fed with the cornflower-3-O-galactoside evidently decreases and the nerve cells apoptosis rate evidently decreases. The hint shows that the cornflower-3-O-galactoside can evidently suppress the hippocampal nerve cell apoptosis induced by H2O2 and play a protection role in the hippocampal nerve cells damaged by H2O2.
Description
Technical field
The present invention relates to the purposes of corn flower-3-O-galactoside, particularly relate to corn flower-3-O-galactoside and improve the application in old cognitive function food or the medicine in preparation.
Background technology
China has stepped into aging society, and the incidence of decline of old cognitive function and nerve degenerative diseases is soaring day by day, and the elderly's physical and mental health and quality of life constituted grave danger.Epidemiologic data shows, present China patients of senile dementia about 6,000,000.Investigation result to 5055 the elderlys more than 55 years old in urban district, Shanghai showed that the senile dementia illness rate reached 4.1% in 2000.Cognitive decrease is the commitment that senile dementia takes place, 2006 to 773 people of community senile more than 60 years old the survey showed that, the cognitive impairment incidence is 16.8%.Therefore, old cognitive impairment and senile dementia have become important " killer " who threatens the senior health and fitness.But still lack the effective measures of improving old memory at present.
Along with the nutrition science progress of research, the biological action of phytochemicals (Phytochemicals), the especially application in the brain health care receive people's attention gradually.Epidemiology survey data and experimental study all confirm; Plurality of vegetables and fruit has very strong scavenging action to free radical; Can significantly improve the age related cognitive decline, and senile dementia, Parkinson's disease and cerebral ischemia re-pouring injured had neuroprotection.But its effective monomer component is not clear and definite as yet, and the mechanism of action that improves old cognitive function is not fully aware of yet.
The drug main that improves old cognitive function at present will comprise that medicine mainly comprises cholinergic agonist, nootropic, calcium antagonist, nerve growth factor (NGF) etc., and wherein drug effect such as lecithin, choline does not confirm as yet; The effect of the nutritious cholinergic nerve of centrum of NGF unit, but molecular weight is big, is difficult for passing through blood brain barrier; Calcium channel blocker such as Nimodipine, flunarizine can play neuroprotection, and improves the brain blood supply.All there is toxic and side effect in various degree in drug therapy, and the expense of taking for a long time is higher.Niacinamide, choline and Cobastab are mainly used in nutrition treatment
1, Cobastab
2, Cobastab
6, Cobastab
12Deng nutrient or be rich in the food of these nutrients, the effectiveness of improving old cognitive function is still imprecise.Therefore, seek a kind of monomer active ingredient that can improve old cognitive function, and then relevant functional food or the medicine of development, for old cognition dysfunction of control and nerve degenerative diseases, all have important scientific meaning and practical value.
Summary of the invention
The objective of the invention is to overcome deficiency of the prior art, provide corn flower-3-O-galactoside to improve old cognitive function Application in Food in preparation.
The objective of the invention is to overcome deficiency of the prior art, provide corn flower-3-O-galactoside to improve the application in the old cognitive function medicine in preparation.
Technical scheme of the present invention is summarized as follows:
Corn flower-3-O-galactoside improves old cognitive function Application in Food in preparation.
Corn flower-3-O-galactoside improves the application in the old cognitive function medicine in preparation.
Experiment showed, with control group (mouse stomach distilled water) and compare that all obviously prolong the dark incubation period of keeping away of corn flower-3-O-galactoside treated animal; The escape latency of mouse in the Morris water maze obviously shortens; Obviously prolong in the platform quadrant duration, the number of times of spanning platform obviously increases.The prompting aged mouse replenishes corn flower-3-O-galactoside can obviously improve the spatial memory ability.With H
2O
2Model group compares, and corn flower-3-O-galactoside group hippocampal neurons supernatant LDH is active significantly to be reduced; The nerve cell apoptosis rate significantly reduces.Prompting corn flower-3-O-galactoside can obviously suppress H
2O
2The hippocampal neurons apoptosis of inducing is to H
2O
2The damage hippocampal neurons has protective effect.
Description of drawings
Fig. 1 is corn flower in the sample-3-O-galactoside thin-layer chromatogram.
Fig. 2 is corn flower in the sample-3-O-galactoside liquid chromatogram.
Fig. 3 is the influence of corn flower-3-O-galactoside to the aged mouse escape latency.(compare with control group,
*P＜0.05)
Fig. 4 is the influence of corn flower-3-O-galactoside to aged mouse platform quadrant swimming time.(compare with control group,
*P＜0.05)
Fig. 5 is the influence of corn flower-3-O-galactoside to aged mouse spanning platform number of times.(compare with control group,
*P＜0.05)
Fig. 6 is that corn flower-3-O-galactoside is to H
2O
2The active influence of damage hippocampal neurons LDH.(compare with control group,
*P＜0.05; With H
2O
2Group compares, #P＜0.05)
Fig. 7 is that corn flower-3-O-galactoside is to H
2O
2The influence of damage hippocampal neurons apoptosis.(compare with control group,
*P＜0.05; With H
2O
2Group compares, #P＜0.05)
The specific embodiment
Below in conjunction with specific embodiment the present invention is further described.Corn flower-3-O-galactoside prepares for this laboratory voluntarily, is accredited as corn flower-3-O-galactoside through mass spectrum.
Effect assessment: corn flower-3-O-galactoside improves the experimental study of aged mouse cognitive function
14 of 15 monthly age SPF kunming mices, male and female half and half.Adaptability is divided into control group and corn flower-3-O-galactoside group (set of monomers), 7 every group by body weight after raising 1w.Control group mice is irritated stomach distilled water; It is 50mg/kg bwd that set of monomers is irritated stomach dosage.Experimental period 6w.
Experimental result shows that (1) compares with control group, and all obviously prolong the dark incubation period of keeping away of set of monomers animal; (2) compare with control group, the escape latency of set of monomers mouse in the Morris water maze obviously shortens; Obviously prolong in the platform quadrant duration, the number of times of spanning platform obviously increases.The prompting aged mouse replenishes corn flower-3-O-galactoside can obviously improve the spatial memory ability.
Table 1 corn flower-3-O-galactoside is kept away dark preclinical influence
to aged mouse
Compare with control group,
*P＜0.05
Effect assessment: corn flower-3-O-galactoside alleviates the experimental study of hippocampal neurons peroxide injury
The hippocampal neurons of the newborn suckling mouse of separation and Culture adds 50 μ mol/L H
2O
2Set up oxidative stress damage model (H
2O
2Model group); In nutrient solution, add 1 μ g/ml corn flower-3-O-galactoside (set of monomers).Measure LDH activity, neuron survival rate and apoptosis rate in the cell conditioned medium liquid.
The result shows, with H
2O
2Model group compares, and set of monomers hippocampal neurons supernatant LDH is active significantly to be reduced; The nerve cell apoptosis rate significantly reduces.Prompting corn flower-3-O-galactoside can obviously suppress H
2O
2The hippocampal neurons apoptosis of inducing is to H
2O
2The damage hippocampal neurons has protective effect.
Corn flower-3-O-galactoside and pharmacy acceptable auxiliary are processed convenient effervescent tablet, chewable tablets, capsule or the parenteral solution that uses by routine techniques.
Corn flower-3-O-galactoside and the auxiliary material that is usually used in preparing functional food are processed convenient liquid beverage, solid beverage, effervescent tablet, chewable tablets or the capsule that uses by routine techniques.
The preparation and the evaluation of corn flower-3-O-galactoside
1, preparation method:
(1) the blueberry extract extracts: with the blueberry is raw material; The concentration of volume percent that adds 20 times of said raw materials quality is that 75% ethanol water is for extracting solvent; 60 ℃ are extracted 5 times, and each 5 hours, decompression concentrated; Obtaining extract, is that 1: 2 ratio is dispersed in the water with said extract with mass ratio;
(2) macroporous resin column chromatography purifying: the product that said step (1) is obtained is through macroporous resin adsorption; The use concentration of volume percent is 90% ethanol water wash-out, and eluent is through the thin-layer chromatography method and combine detection, and the component of corn flower 3-O-galactoside is rich in collection; With its merging; In temperature≤60 ℃, vacuum is that 0.06～0.08MPa decompression concentrates, corn flower 3-O-galactoside bullion;
(3) polyamide column chromatography purification: is that 1: 5 ratio is dispersed to and carries out the polyamide adsorpting column chromatography in the polyamide with said concentrate in mass ratio; The use concentration of volume percent is 85% ethanol water wash-out; Through thin-layer chromatography, collect the component be rich in corn flower-3-O-galactoside, with eluent in temperature≤60 ℃; Vacuum is 0.06～0.08MPa decompression concentrate drying, obtains corn flower-3-O-galactoside;
(4) corn flower-3-O-galactoside is refining: is that 1: 5 ratio is dispersed to and carries out the polyamide adsorpting column chromatography in the polyamide with gained corn flower-3-O-galactoside in mass ratio; The use concentration of volume percent is 75% ethanol water wash-out; Detect through thin-layer chromatography binding analysis type HPLC; The component of corn flower-3-O-galactoside is rich in collection, and 50 ℃ of temperature, vacuum is 0.06～0.08MPa decompression concentrate drying with eluent; Obtain corn flower-3-O-galactoside, its content is 90%.
2, identify
The anthocyanin monomer of this prepared in laboratory is the aubergine crystal, and is water-soluble, is soluble in methyl alcohol; M.p.181～183 ℃.Measure its molecular ion peak [M] through electrospray ionization mass spectrometry (ESI-MS)
+=449.08, be C in conjunction with its molecular formula of NMR inferred from input data
11H
21O
11With anthocyanin data contrast in its carbon spectrum data and the document, can find out that this compound parent carbon skeleton has the characteristic of cyanidin; This compound
1H,
13The C-NMR data are consistent with the data contrast of corn flower-3-O-galactoside in the document, so we confirm that this monomer is corn flower-3-O-galactoside.
The hydrogen spectrum of table 1 anthocyanin corn flower-3-O-galactoside, carbon spectrum data
Raw material in this instance can also be selected blackberry, blueberry, the red certain kind of berries, mulberry fruit fruit etc. for use.
The HPLC testing conditions of corn flower-3-O-galactoside:
Chromatographic column: Waters AtlantisTMdC18 (150mm * 4.6mm i.d., 5 μ m)
Detect wavelength: 520nm
Flow velocity: 1ml/min
Sample size: 20 μ l
Column temperature: 25 ℃
Flowing phase: acetonitrile (contains 0.2% trifluoroacetic acid, v/v): H
2O=3: 1
Claims (2)
1. corn flower-3-O-galactoside improves old cognitive function Application in Food as unique active component in preparation.
2. corn flower-3-O-galactoside improves the application in the old cognitive function medicine as unique active component in preparation.
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|---|---|---|---|
| CN2009100704992A CN101669637B (en) | 2009-09-18 | 2009-09-18 | Application of cornflower-3-O-galactoside in preparing foods or medicaments for improving cognitive functions of old people |
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|---|---|---|---|
| CN2009100704992A CN101669637B (en) | 2009-09-18 | 2009-09-18 | Application of cornflower-3-O-galactoside in preparing foods or medicaments for improving cognitive functions of old people |
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| Publication Number | Publication Date |
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| CN101669637A CN101669637A (en) | 2010-03-17 |
| CN101669637B true CN101669637B (en) | 2012-05-23 |
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| CN109549946B (en) * | 2019-01-08 | 2020-12-18 | 牡丹江医学院 | Medicine for treating pharyngitis and preparation method thereof |
| CN112076202A (en) * | 2020-09-22 | 2020-12-15 | 中国农业大学 | Pharmaceutical composition and application thereof in preparation of medicines for preventing and/or treating Alzheimer's disease |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1985867A (en) * | 2006-12-25 | 2007-06-27 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Medicine composition for improving cognitive function of senile organism |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1985867A (en) * | 2006-12-25 | 2007-06-27 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Medicine composition for improving cognitive function of senile organism |
Non-Patent Citations (2)
| Title |
|---|
| Wei Zhang, et al.oxygen radical absorbing capacity of phenolics in blueberries, cranberries, chokeberries and lingonberries.《Journal of Agriculture and food chemistry》.2002,第51卷(第2期),502-509. * |
| 房恒通,等.复合营养素对老龄大鼠认知与运动功能的干预作用及其机制研究.《营养学报》.2007,第29卷(第5期),422-425. * |
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