CN101666747A - Array fibre evanescent wave biosensor system - Google Patents
Array fibre evanescent wave biosensor system Download PDFInfo
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- CN101666747A CN101666747A CN200810119650A CN200810119650A CN101666747A CN 101666747 A CN101666747 A CN 101666747A CN 200810119650 A CN200810119650 A CN 200810119650A CN 200810119650 A CN200810119650 A CN 200810119650A CN 101666747 A CN101666747 A CN 101666747A
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Abstract
The invention discloses an array fibre evanescent wave biosensor system, which comprises a laser transmitting device (1), laser transmission optical paths (2, 601 and 603), an optical fibre probe (9),fluorescence receiving optical paths (602 and 603), a photoelectric detector (4) and a processing unit (11), wherein the input ends of the laser transmission optical paths (2, 601 and 603) are coupled with the laser transmitting device (1), while the output ends are coupled to the optical fibre probe (9); the input ends of the fluorescence receiving optical paths (602, 603) are coupled with the optical fibre probe (9), while the output ends are coupled to the photoelectric detector (4); the processing unit (11) is connected with the photoelectric detector (4) and the laser transmitting device(1); and the laser transmission optical paths (2, 601 and 603) and the fluorescence receiving optical paths (602 and 603) are at least partially formed by optical fibres. The array fibre evanescent wave biosensor system has the advantages of simple structure, high optical transmission efficiency, compact instruments and miniaturization.
Description
Technical field
The present invention relates to a kind of array fibre evanescent wave biosensor system, relate in particular to a kind of array fibre evanescent wave biosensor system that is used for testing environment organic contamination substrate concentration.
Background technology
Fibre evanescent wave biosensor system produces evanescent wave when utilizing light wave to transmit in the total reflection mode in the residing medium of fibre-optical probe in optical fiber, this evanescent wave can excite by absorption or with biological compatible reaction and be connected the biological substance that is marked with fluorescence molecule on the detecting head surface, thereby realize detecting the purpose of target substance concentration to be measured.Usually the penetration depth of evanescent wave field has only tens nanometer to the hundreds of nanometer, be incorporated into the fluorescence that the fluorescence molecule in the scope of evanescent wave field sends so fibre evanescent wave biosensor system can only detect, and free fluorescence molecule is contributed to testing result in the solution very little.Therefore biologic sensor for fast travelling waves of optical fibre have highly sensitive, biologic specificity is strong; Simple to operate, measuring speed is fast; Can monitor the dynamic process of biological respinse; Complete machine can miniaturization etc. characteristics, thereby make it obtain in fields such as biomedical research, food inspection, environmental monitorings using widely.
Traditional fibre evanescent wave biosensor system mainly is to utilize optical fiber as probe and optical information hop, and utilizes the optical fractionation components and parts in a large number in the parts such as collection of signal.Transmission, collection and the detection of the fluorescence that goes out to exciting light with from fibre-optical probe one end-fire all is to adopt conventional optical system, generally includes optical elements such as catoptron, lens combination, chopper.
Therefore, the shortcoming of traditional fibre evanescent wave biosensor system is: system complex; Energy loss is big; Element positioning requirements height; Big, the difficult instrument miniaturization of realizing takes up room; The light path arrangement flexibility ratio is low, and the adjusting difficulty is big etc.
Summary of the invention
The present invention proposes a kind of array fibre evanescent wave biosensor system that is used for testing environment organic contamination substrate concentration, purpose is all to utilize optical fiber to finish with transmission the collection of the transmission of exciting light and fluorescence.
According to an aspect of the present invention, proposed a kind of array fibre evanescent wave biosensor system, having comprised: laser beam emitting device (1); Laser Transmission light path (2,601,603); Fibre-optical probe (9); Fluorescence receiving light path (602,603); Photodetector (4); And processing unit (11).
Wherein, the input end of Laser Transmission light path (2,601,603) and laser beam emitting device (1) coupling, output terminal is coupled to fibre-optical probe (9); The input end of fluorescence receiving light path (602,603) and fibre-optical probe (9) coupling, output terminal is coupled to photodetector (4); Processing unit (11) is connected with photodetector (4) and laser beam emitting device (1).
Wherein, Laser Transmission light path (2,601,603) and fluorescence receiving light path (602,603) are made of optical fiber at least in part.
Wherein, sensing system comprises a plurality of described fibre-optical probes (9); The Laser Transmission light path comprises: first laser optical path part (2) will be divided into from the single channel laser beam of laser beam emitting device (1) and the corresponding multi-path laser bundle of a plurality of fibre-optical probes (9); And the second laser optical path part (601,603), constituting by optical fiber, its two ends be coupled respectively first laser optical path part (2) and a plurality of fibre-optical probe (9) are to transmit the multi-path laser bundle.Wherein, fluorescence receiving light path (602,603) is made of optical fiber; Second laser optical path part (601,603) and fluorescence receiving light path (602, the 603) one of Laser Transmission light path constitute single multi-module optical fiber coupler (6).In addition, the single-mode optical-fibre coupler of first laser optical path of Laser Transmission light path part (2) for constituting by optical fiber.Wherein, be provided with fluorescent optical filter (5) between photodetector (4) and the fluorescence receiving light path (602,603).In addition, sensing system also comprises sample flow path, and fibre-optical probe (9) is arranged in the stream.Wherein, sample flow path comprises sample cell (10), the inlet portion (13) that is connected with sample cell (10) respectively and export department (8) and is arranged on pump (14) in the inlet portion (13).Wherein, processing unit (11) comprising: lock-in amplifier (111), be connected with laser beam emitting device (1) with photodetector (4), and lock-in amplifier (111) is used for signal to photodetector (4) output and filters and amplify; Calculation element (112) is connected with lock-in amplifier (111), gathers from the electric signal of lock-in amplifier (111) output and to electric signal and carries out data processing.Wherein, the calculation element (112) of processing unit (11) is connected to the operation of the pump (14) of sample flow path with control pump (14).In addition, fibre-optical probe (9) is the tapered multimode optical fiber probe of combination.Wherein, the fiber core surface of fibre-optical probe (9) is fixed with biological identification molecule.
Array fibre evanescent wave sensor-based system of the present invention is realized the transmission of exciting light and the collection and the transmission of fluorescence by optical fiber, so the optical fractionation element is few, simple in structure, light transmission efficiency height, has realized the compactedness and the miniaturization of instrument; Simultaneously, this instrument adopts array fibre probe form, can realize the detection of several samples simultaneously.
Description of drawings
Accompanying drawing described herein is used to provide further understanding of the present invention, constitutes the application's a part, and illustrative examples of the present invention and explanation thereof are used to explain the present invention, do not constitute improper qualification of the present invention.In the accompanying drawings:
Fig. 1 is the block diagram according to the array fibre evanescent wave biosensor system of the embodiment of the invention;
Fig. 2 is the synoptic diagram of fibre-optical probe 9 shown in Figure 1;
Fig. 3 is the synoptic diagram of single multi-module optical fiber coupler 6 shown in Figure 1; And
Fig. 4 is the synoptic diagram of single-mode optical-fibre coupler 2 shown in Figure 1.
Embodiment
Below with reference to accompanying drawing, describe the specific embodiment of the present invention in detail.
Fig. 1 is the block diagram according to the array fibre evanescent wave biosensor system of the embodiment of the invention.As shown in Figure 1, the array fibre evanescent wave biosensor system according to the embodiment of the invention comprises: laser beam emitting device 1; Laser Transmission light path 2,601,603; Fibre-optical probe 9; Fluorescence receiving light path 602,603; Photodetector 4; And processing unit 11.
Wherein, the input end of Laser Transmission light path 2,601,603 and laser beam emitting device 1 coupling, output terminal is coupled to fibre-optical probe 9; The input end of fluorescence receiving light path 602,603 and fibre-optical probe 9 couplings, output terminal is coupled to photodetector 4; Processing unit 11 is connected with photodetector 4 and laser beam emitting device 1.
Wherein, Laser Transmission light path 2,601,603 and fluorescence receiving light path 602,603 are made of optical fiber at least in part.
Wherein, sensor-based system comprises a plurality of described fibre-optical probes 9; The Laser Transmission light path comprises: the first laser optical path part 2 will be divided into from the single channel laser beam of laser beam emitting device 1 and a plurality of fibre-optical probe 9 corresponding multi-path laser bundles; And the second laser optical path part 601,603, constitute by optical fiber, wherein, 601 and 602 be coupled the respectively first laser optical path part 2 and a plurality of fibre-optical probes 9 are with transmission multi-path laser bundle.Wherein, fluorescence receiving light path 602,603 is made of optical fiber; The second laser optical path part 601,603 of Laser Transmission light path constitutes single multi-module optical fiber coupler 6 with fluorescence receiving light path 602,603 one.In addition, the single-mode optical-fibre coupler of the first laser optical path part 2 for constituting of Laser Transmission light path by optical fiber.Wherein, be provided with fluorescence filter sheet 5 between photodetector 4 and the fluorescence receiving light path 602,603.In addition, sensor-based system also comprises sample flow path, and fibre-optical probe 9 is arranged in the stream.Wherein, sample flow path comprises sample cell 10, the inlet portion 13 that is connected with sample cell 10 respectively and export department 8 and is arranged on pump 14 in the inlet portion 13.Wherein, processing unit 11 comprises: lock-in amplifier 111, be connected with laser beam emitting device 1 with photodetector 4, and lock-in amplifier 111 is used for signal to photodetector 4 output and filters and amplify; Calculation element 112 is connected with lock-in amplifier 111, gathers from the electric signal of lock-in amplifier 111 outputs and to electric signal and carries out data processing.Wherein, the calculation element 112 of processing unit 11 is connected to the operation of the pump 14 of sample flow path with control pump 14.In addition, fibre-optical probe 9 is the tapered multimode optical fiber probe of combination.Wherein, the fiber core surface of fibre-optical probe 9 is fixed with biological identification molecule.
As shown in Figure 1, array fibre evanescent wave biosensor system comprises that laser beam emitting device, Laser Transmission and fluorescence receiving light path, sample flow path, fluorescence harvester and data processing equipment constitute according to another embodiment of the present invention.Wherein laser beam emitting device is a pulsed laser 1; Laser Transmission and fluorescence receiving light path 1 * 3 single-mode optical-fibre coupler 2, single multi-module optical fiber coupler 6 and fibre-optical probe 9; Sample flow path contains sample cell 10, peristaltic pump 14, and sample cell inlet 8 and sample cell outlet 13 are arranged on the sample cell; Fluorescence collection and data processing equipment contain fluorescent optical filter 5, photodetector 4, lock-in amplifier 111 and calculation element 112.
As seen from Figure 1, on excitation light path, the laser that pulsed laser 1 sends enters 3 single-mode fibers 601 in single multi-module optical fiber coupler 6 respectively by 1 * 3 single-mode optical-fibre coupler 2, multimode optical fiber 603 through single multi-module optical fiber coupler transmits then, enter fibre-optical probe 9 by the connector 7 that connects single multi-module optical fiber coupler 6 and fibre-optical probe 9, produce evanescent wave in probe 9 near surfaces zone, be connected the probe 9 lip-deep biological substances that are marked with fluorescence molecule to excite.The part fluorescence probe 9 that is coupled back enters the multimode optical fiber 603 of single multi-module optical fiber coupler 6 by connector 7, enters multimode optical fiber 602 then, penetrates at the other end of multimode optical fiber 602.Fluorescent optical filter 5 filterings exciting light reflection or that lose, and most of fluorescence is seen through, the fluorescence that sees through is surveyed by photodetector 4 (photodiode) and is converted light signal to electric signal, this electric signal amplifies through lock-in amplifier 111 then, gathers the line data analysis of going forward side by side by calculation element 112.Pulsed laser 1 provides the same frequency signal as the reference signal for lock-in amplifier 111 simultaneously.
As shown in Figure 2, fibre-optical probe 9 adopts the tapered form of combination, and the part covering 901 of optical fiber is removed, and fibre core 902 usefulness hydrofluorite corrode into band tapered structure 903, and making up tapered probe 904 finishinges has biological identification molecule.Like this, can improve the incident angle of incident light on the one hand, make incident light increase, can increase the number of times that excites, thereby can inspire more fluorescent energy fluorescence molecule at fibre-optical probe 9 internal reflection number of times; On the other hand, the fluorescence that is coupled into probe with the high-order mode form can be changed into low-order mode, reduce the fluorescence losses that does not match and cause because of pattern, with collection and the transfer efficiency of further raising system fluorescence.
As shown in Figure 3, single multi-module optical fiber coupler 6 is to adopt welding commonly used at present to draw the awl method to make, wherein, the 601st, single-mode fiber, 602 and 603 is multimode optical fibers.Single-mode fiber 601 is that exciting light is introduced multimode optical fiber, and single-mode fiber only transmits a kind of light of pattern, can stop the loss of light effectively.And the fluorescence receiving light path adopts multimode optical fiber, not only can increase the fluorescence quality that detecting head surface can be excited, and can improve the coupling efficiency of fluorescence, thus the sensitivity and the signal to noise ratio (S/N ratio) of raising system.
Fibre-optical probe 9 adopts dismountable connector 7 to be connected with single multi-module optical fiber coupler 6.
Identical with the using method of traditional biosensor of full fiber optic evanescent wave, array fibre evanescent wave sensor-based system of the present invention is before detection, need to modify biological identification molecule at probe 9, then three different probes 9 are placed sample cell 10, and will link to each other with single multi-module optical fiber coupler 6 respectively with connector 7.Open peristaltic pump 14, testing sample is sucked sample cell 10, behind the reaction certain hour, open laser beam emitting device 1, exciting light will enter probe 9 in the total reflection mode, the evanescent wave that laser beam produces during multihop propagation in probe 9 excites the fluorescence molecule of detecting head surface, fluorescence molecule sends fluorescence, part is coupled back and pops one's head in 9, after multimode optical fiber 603 and 602 transmission, penetrate from multimode optical fiber 602 other ends, through the fluorescent optical filter 5 laggard photodetector 4 of going into that filters, and be converted into the electric signal that is directly proportional with fluorescence signal intensity, gathered by calculation element 112 again.The proportional relation of the concentration of electric signal that calculation element 112 collects and detecting head surface measured matter is by the size of analyzing electric signal can obtain the popping one's head in concentration of 9 surperficial measured matters.
As shown in Figure 1, array fibre evanescent wave sensor-based system according to still another embodiment of the invention, LASER Light Source is that centre wavelength is the semiconductor laser 1 of 635nm, output power is 15mW, laser instrument is 1: 1: 1 to single-mode optical-fibre coupler 201 coupling efficiencies 〉=beam split rate of 202: 203: 204 of 80%, three single-mode fiber.The optical fiber of single multi-module optical fiber coupler 6 is silica fibre, and wherein multimode optical fiber 602 and 603 diameter are 600 microns, and numerical aperture is 0.22.Transmitance≤10 of 4 pairs of exciting lights of fluorescent optical filter
-7, to transmitance 〉=80% of fluorescence.Photodetector 4 is a photodiode.
During measurement, the biological sample solution of Cy5.5 fluorescence molecule that in sample cell 10, injected mark, every fibre-optical probe all reaches 10 to the detection sensitivity of Cy5.5 fluorescence molecule solution among the embodiment
-9Mol/L.The detection sensitivity difference of each fibre-optical probe is less than 5% simultaneously.Finish first order fluorescence input required time and be less than 15min.
The above is the preferred embodiments of the present invention only, is not limited to the present invention, and for a person skilled in the art, the present invention can have various changes and variation.Within the spirit and principles in the present invention all, any modification of being done, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (11)
1. array fibre evanescent wave biosensor system that is used for testing environment organic contamination substrate concentration, comprise: laser beam emitting device (1), Laser Transmission light path (2,601,603), fibre-optical probe (9), fluorescence receiving light path (602,603), photodetector (4) and processing unit (11), wherein:
The input end of described Laser Transmission light path (2,601,603) and described laser beam emitting device (1) coupling, output terminal is coupled to described fibre-optical probe (9);
The input end of described fluorescence receiving light path (602,603) and described fibre-optical probe (9) coupling, output terminal is coupled to described photodetector (4);
Described processing unit (11) is connected with described photodetector (4) and described laser beam emitting device (1);
It is characterized in that described Laser Transmission light path (2,601,603) and described fluorescence receiving light path (602,603) are made of optical fiber at least in part.
2. sensing system according to claim 1 is characterized in that:
Described sensor-based system comprises a plurality of described fibre-optical probes (9);
Described Laser Transmission light path comprises:
First laser optical path part (2) will be divided into from the single channel laser beam of described laser beam emitting device (1) and the corresponding multi-path laser bundle of a plurality of described fibre-optical probes (9); And
Second laser optical path part (601,603) is made of optical fiber, and its two ends be coupled respectively described first laser optical path part (2) and a plurality of described fibre-optical probe (9) are to transmit described multi-path laser bundle.
3. sensing system according to claim 2 is characterized in that:
Described fluorescence receiving light path (602,603) is made of optical fiber;
Described second laser optical path part (601,603) and described fluorescence receiving light path (602, the 603) one of described Laser Transmission light path constitute single multi-module optical fiber coupler (6).
4. sensing system according to claim 3 is characterized in that:
The single-mode optical-fibre coupler of described first laser optical path part (2) for constituting of described Laser Transmission light path by optical fiber.
5. according to described sensing system one of in the claim 1 to 4, it is characterized in that:
Be provided with fluorescent optical filter (5) between described photodetector (4) and the described fluorescence receiving light path (602,603).
6. according to described sensing system one of in the claim 1 to 4, it is characterized in that:
Described sensor-based system also comprises sample flow path, and described fibre-optical probe (9) is arranged in the described stream.
7. sensing system according to claim 6 is characterized in that:
Described sample flow path comprises sample cell (10), the inlet portion (13) that is connected with described sample cell (10) respectively and export department (8) and is arranged on pump (14) in the described inlet portion (13).
8. according to described sensor-based system one of in the claim 1 to 4, it is characterized in that:
Described processing unit (11) comprising:
Lock-in amplifier (111) is connected with described laser beam emitting device (1) with described photodetector (4), and described lock-in amplifier (111) is used for signal to described photodetector (4) output and filters and amplify;
Calculation element (112) is connected with described lock-in amplifier (111), gathers from the electric signal of described lock-in amplifier (111) output and to described electric signal and carries out data processing.
9. sensing system according to claim 8 is characterized in that:
Described sensing system also comprises sample flow path, and described sample flow path comprises sample cell (10), the inlet portion (13) that is connected with described sample cell (10) respectively and export department (8) and is arranged on pump (14) in the described inlet portion (13);
The described calculation element (112) of described processing unit (11) is connected to the described pump (14) of described sample flow path to control the operation of described pump (14).
10. according to described sensing system one of in the claim 1 to 4, it is characterized in that described fibre-optical probe (9) is the tapered multimode optical fiber probe of combination.
11., it is characterized in that the fiber core surface of described fibre-optical probe (9) is fixed with biological identification molecule according to described sensing system one of in the claim 1 to 4.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101893562A (en) * | 2010-06-22 | 2010-11-24 | 浙江大学 | High-sensitivity integrated optical waveguide sensor based on digital droplet sample introduction channel |
| CN101949849A (en) * | 2010-09-08 | 2011-01-19 | 华中科技大学 | Light-activated positioning microscopic imaging system based on optical fiber evanescent field illuminator |
| CN104614350A (en) * | 2014-12-25 | 2015-05-13 | 北京农业信息技术研究中心 | Detector for aflatoxin |
| CN105606583A (en) * | 2016-03-25 | 2016-05-25 | 北京首量科技有限公司 | Optical fiber detection system for biological detection |
| CN106198464A (en) * | 2015-05-08 | 2016-12-07 | 罗强 | A kind of laser-induced fluorescence (LIF) food detector |
| CN106990086A (en) * | 2017-06-06 | 2017-07-28 | 中国人民大学 | A kind of multichannel Evanescent Wave All-fiber biology sensor |
| CN110082334A (en) * | 2019-05-30 | 2019-08-02 | 华侨大学 | A kind of multichannel optical fiber fluorescent optical sensor |
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2008
- 2008-09-04 CN CN200810119650A patent/CN101666747A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101893562A (en) * | 2010-06-22 | 2010-11-24 | 浙江大学 | High-sensitivity integrated optical waveguide sensor based on digital droplet sample introduction channel |
| CN101893562B (en) * | 2010-06-22 | 2011-12-21 | 浙江大学 | High-sensitivity integrated optical waveguide sensor based on digital droplet sample introduction channel |
| CN101949849A (en) * | 2010-09-08 | 2011-01-19 | 华中科技大学 | Light-activated positioning microscopic imaging system based on optical fiber evanescent field illuminator |
| CN101949849B (en) * | 2010-09-08 | 2011-09-21 | 华中科技大学 | Light-activated positioning microscopic imaging system based on optical fiber evanescent field illuminator |
| CN104614350A (en) * | 2014-12-25 | 2015-05-13 | 北京农业信息技术研究中心 | Detector for aflatoxin |
| CN106198464A (en) * | 2015-05-08 | 2016-12-07 | 罗强 | A kind of laser-induced fluorescence (LIF) food detector |
| CN105606583A (en) * | 2016-03-25 | 2016-05-25 | 北京首量科技有限公司 | Optical fiber detection system for biological detection |
| CN106990086A (en) * | 2017-06-06 | 2017-07-28 | 中国人民大学 | A kind of multichannel Evanescent Wave All-fiber biology sensor |
| CN106990086B (en) * | 2017-06-06 | 2020-02-28 | 中国人民大学 | Multi-channel evanescent wave all-fiber biosensor |
| CN110082334A (en) * | 2019-05-30 | 2019-08-02 | 华侨大学 | A kind of multichannel optical fiber fluorescent optical sensor |
| CN110082334B (en) * | 2019-05-30 | 2024-02-23 | 华侨大学 | Multichannel optical fiber fluorescence sensor |
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