CN101665805A - Method for producing microbial flocculant by using aquaculture wastewater - Google Patents
Method for producing microbial flocculant by using aquaculture wastewater Download PDFInfo
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- CN101665805A CN101665805A CN200910190894A CN200910190894A CN101665805A CN 101665805 A CN101665805 A CN 101665805A CN 200910190894 A CN200910190894 A CN 200910190894A CN 200910190894 A CN200910190894 A CN 200910190894A CN 101665805 A CN101665805 A CN 101665805A
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Abstract
The invention discloses a method for producing microbial flocculant by using aquaculture wastewater, which comprises the following steps: firstly, separating and screening out a bacterial strain of flocculant- producing bacterium, then implanting the bacterial strain into a culture medium containing beef extract, peptone and table salt so as to culture bacterial strain seed solution by fermentation, then adding glucose (as supplementary carbon source) and/or (NH4)2SO4 (as supplementary nitrogen source) in the aquaculture wastewater with COD being 1000-10000mg/l so as to prepare a fermentationmedium of the aquaculture wastewater, adding a specified volume of bacterial strain seed solution, and aerating, fermenting and culturing for 36-48 hours at shaker rotating speed of 120-150r/min and temperature of 25-35 DEG C, thus obtaining the microbial flocculant product. The product is used for treating wastewater, not only can be used singly, but also can be combined with inorganic flocculant, calcium ion and iron ion for use, and can achieve ideal flocculation effect when being used singly for treating domestic sewage. The method can fully utilize the carbon source and the nitrogen source in the aquaculture wastewater and greatly save the dosage of the carbon source and the nitrogen source, has simpe technological process, low cost and good using effect, can use waste to treat waste,and is easy to realize industrialized mass production.
Description
One, technical field
The invention belongs to and use microorganism to prepare compound or method for compositions, particularly use microorganism culturing to produce the method for microbial flocculant.
Two, background technology
Flocculation agent is widely used in the industrial circles such as sewage disposal, foodstuff production and fermentation.Usually flocculation agent is divided into inorganic flocculating agent, organic synthesis polymeric flocculant and biopolymer flocculation agent three major types.Microbial flocculant belongs to the category of biopolymer flocculation agent.Microbial flocculant is that microorganism is under Incubation Condition, the meta-bolites that growth metabolism produces to certain phase with flocculation activity, it is a kind ofly can make in the water body that suspended particle, somatic cells and the colloidal particle etc. that are difficult for degraded condense, settled biopolymer polymkeric substance, it is easy to separate, the subsidence rate height, degraded easily, and its degraded product is nontoxic, can not produce secondary pollution to environment, having good purification and decolorization, is the flocculation agent of a kind of wide adaptability, highly effective and safe.
At present, the research and development to microbial flocculant both at home and abroad are in laboratory stage mostly, also fail to realize suitability for industrialized production, its major cause be output cross low or production cost too high.In the prior art, there is a correlation technique CN101225405A open on July 23rd, 2008, it is as carbon source with starch wastewater, utilize compound nitrogen source to produce microbial flocculant, technology is that COD is that the starch wastewater of 1.2-1.5 ten thousand mg/L is after the pre-fermentation of aeration, add the nitrogenous source of 200-500mg/L, the phosphorus of 300-800mg/L, the sal epsom of 10-25mg/L, transfer to pH6.0-8.0, insert the Klebsiella seed liquor behind the autoclaving, through biological shaking table aeration, results flocculation agent crude product behind the cultivation 24-44h; It also discloses the using method of this microbial flocculant, and with it and the compound use of calcium ion, aluminum ion or iron ion, wherein calcium ion adds concentration 72-180mg/L, and aluminum ion or iron ion are all in the 0.2-0.6mg/L scope.It is said that this microbial flocculant can obtain high flocculation efficiency and few dosage, can reduce the water treatment expense when carrying out water treatment.But there is following major defect in above-mentioned correlation technique CN101225405A: can only provide carbon source with starch wastewater, it is limited to originate; Nitrogenous source is the mixture of sulfuric acid amine and urea, and the amount of adding is bigger, also will add a certain amount of inorganic salt, and cost is increased; Described Klebsiella seed liquor, its technology of preparing is unclear, implements difficulty.
Three, summary of the invention
The objective of the invention is to: at the above-mentioned defective of prior art existence, plan with the breeding wastewater that is rich in N, P, K as carbon, nitrogenous source, a kind of method of producing microbial flocculant with breeding wastewater is provided, it is more cheap extensively to make it carbon, nitrogenous source source, it is lower to cultivate cost, and result of use is better, easier realization commercial scale production, energy comprehensive utilization of waste materials simultaneously, the treatment of wastes with processes of wastes against one another.
Adopt following technology side to realize purpose of the present invention.
A kind of method of producing microbial flocculant with breeding wastewater is made up of the separation screening of bacterium for producing flocculant, the preparation of bacterial strain seed liquor and the three step processes of obtaining of microbial flocculant.
One, the separation screening of bacterium for producing flocculant
Get the active sludge of sewage work, through dilution, enrichment culture, line separates, filter out the single bacterium colony of vigorous, translucent, the thickness of growth, smooth bumps, determine to obtain purer bacterial strain with microscopy again, with the flocculating effect experiment primary dcreening operation bacterial strain is carried out multiple sieve at last, obtain the bacterium for producing flocculant bacterial strain.
Two, the preparation of bacterial strain seed liquor
The first ratio of 4～6 parts of 1000 parts in water, 2～4 parts of extractum carniss, 7～12 parts of peptones and salt by weight, secure ph is the seed culture medium of 6.0-8.0, again the bacterium for producing flocculant bacterial strain is implanted wherein, cultivate 24h at the biological shaking speed bottom fermentation of 25-35 ℃, 120-150r/min, optical density(OD) is that 1.4～1.6 fermented liquid is the bacterial strain seed liquor.
Three, microbial flocculant obtains
The breeding wastewater that is 1000-10000mg/L by 1 liter of COD concentration adds 0-4g glucose carbon source, adding 0-1g (NH as a supplement earlier
4)
2SO
4The ratio of nitrogenous source as a supplement, making initial pH is the breeding wastewater fermention medium of 6.0-8.0, autoclaving, inoculum size by 100ml breeding wastewater fermention medium adding bacterial strain seed liquor 0.5-2ml is seeded to seed liquor in the cultured manure water fermention medium again, aerated fermentation was cultivated 36-48 hour under 25-35 ℃, 120-150r/min shaking speed at last, and the supernatant liquid of acquisition is the microbial flocculant product.
In microbial flocculant production method of the present invention, described breeding wastewater is the composite waste after farm animal excrement such as pig, ox, sheep, chicken, duck and the plant's flushing, preferably Wastewater from Pig Farm.
Be used for wastewater treatment according to the microbial flocculant product that production method of the present invention obtained, can use separately, also can with inorganic flocculating agent, calcium ion, the compound use of iron ion.Independent when handling sanitary sewage with just reaching the ideal flocculating effect.
Production method of the present invention, can make full use of the carbon source and the nitrogenous source that exist in the breeding wastewater, saved the carbon in the fermention medium greatly, the dosage of nitrogenous source, compared with prior art to add the carbon of 50-90% less, nitrogenous source, make the production cost of microbial flocculant reduce 60-80% at least, simultaneously, application experiment shows, the flocculating rate that the microbial flocculant that adopts production method of the present invention to make is handled kaolin suspension waste water can reach more than 95%, and the water treatment expense also can significantly reduce (can reduce about 75-85%), and its technological process is simple, cost is low, result of use is good, and the energy treatment of wastes with processes of wastes against one another realizes commercial scale production easily.
Four, embodiment
The embodiment that enumerates a plurality of cultured manure water fermention mediums below specifies the result of use of the microbial flocculant that production method of the present invention makes.
1, substratum: 1 liter of Wastewater from Pig Farm (COD:1000mg/L), glucose 4g; Ammonium sulfate 0.5g; Initial pH is 6.0, cultivates 48h.Institute's produce flocculant can reach about 98% kaolinic flocculating rate.
2, substratum: 1 liter of Wastewater from Pig Farm (COD:5000mg/L), glucose 2g; Ammonium sulfate 0.5g; Initial pH is 7.0, cultivates 48h.Institute's produce flocculant can reach about 95% kaolinic flocculating rate.
3, substratum: 1 liter of Wastewater from Pig Farm (COD:10000mg/L), ammonium sulfate 0.2g; Initial pH is 6.0, cultivates 48h.Institute's produce flocculant can reach about 93% kaolinic flocculating rate.
4, substratum: 1 liter of duckery's waste water (COD:3000mg/L), glucose 4g; Initial pH is 7.0, cultivates 36h.Institute's produce flocculant can reach about 95% kaolinic flocculating rate.
Claims (2)
1, a kind of method of producing microbial flocculant with breeding wastewater is made up of the separation screening of bacterium for producing flocculant, the preparation of bacterial strain seed liquor and the three step processes of obtaining of microbial flocculant, it is characterized in that:
1) separation screening of bacterium for producing flocculant is an active sludge of getting sewage work, through dilution, enrichment culture, line separates, filter out the single bacterium colony of vigorous, translucent, the thickness of growth, smooth bumps, determine to obtain purer bacterial strain with microscopy again, with the flocculating effect experiment primary dcreening operation bacterial strain is carried out multiple sieve at last, obtain the bacterium for producing flocculant bacterial strain;
2) preparation of bacterial strain seed liquor is the first ratio of 4～6 parts of 1000 parts in water, 2～4 parts of extractum carniss, 7～12 parts of peptones and salt by weight, secure ph is the seed culture medium of 6.0-8.0, again the bacterium for producing flocculant bacterial strain is implanted wherein, cultivate 24h at the biological shaking speed bottom fermentation of 25-35 ℃, 120-150r/min, optical density(OD) is that 1.4～1.6 fermented liquid is the bacterial strain seed liquor;
3) obtaining of microbial flocculant is that the breeding wastewater that elder generation is 1000-10000mg/L by 1 liter of COD adds 0-4g glucose carbon source, adding 0-1g (NH as a supplement
4)
2SO
4The ratio of nitrogenous source as a supplement, making initial pH is the breeding wastewater fermention medium of 6.0-8.0, autoclaving, inoculum size by 100ml breeding wastewater fermention medium adding bacterial strain seed liquor 0.5-2ml is seeded to seed liquor in the cultured manure water fermention medium again, aerated fermentation was cultivated 36-48 hour under 25-35 ℃, 120-150r/min shaking speed at last, and the supernatant liquid of acquisition is the microbial flocculant product.
2,, it is characterized in that said breeding wastewater is a Wastewater from Pig Farm according to the described production method of claim 1.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101870958A (en) * | 2010-05-10 | 2010-10-27 | 新疆农业科学院微生物应用研究所 | Paenibacillus and application thereof to environmental engineering |
| CN102719482A (en) * | 2012-02-28 | 2012-10-10 | 湖南大学 | Method for producing microbial flocculant by utilizing culture wastewater and residual activated sludge |
| CN102925489A (en) * | 2012-10-10 | 2013-02-13 | 临安天川环保科技有限公司 | Preparation method of microbial flocculant |
| CN102992457A (en) * | 2012-11-25 | 2013-03-27 | 溧阳市天目湖保健品有限公司 | Method for producing coagulant aid through utilizing cow dung |
| CN103184239A (en) * | 2013-03-11 | 2013-07-03 | 宁波飞日水产实业有限公司 | Method for producing microbial flocculant by using surimi processing waste water |
| CN103451232A (en) * | 2012-05-28 | 2013-12-18 | 浙江树人大学 | Preparation method and use method of self-circulation microbial flocculant |
| CN105296400A (en) * | 2015-11-26 | 2016-02-03 | 南京贝克特环保科技有限公司 | Microbial nutrient for biological coagulation treatment of feces in pig breeding farm and production process of microbial nutrient |
| CN105296401A (en) * | 2015-11-26 | 2016-02-03 | 南京贝克特环保科技有限公司 | Biological coagulation treatment microorganism nutritional agent for feces in dairy farm and production process of nutritional agent |
-
2009
- 2009-09-18 CN CN200910190894A patent/CN101665805A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101870958A (en) * | 2010-05-10 | 2010-10-27 | 新疆农业科学院微生物应用研究所 | Paenibacillus and application thereof to environmental engineering |
| CN101870958B (en) * | 2010-05-10 | 2012-05-23 | 新疆农业科学院微生物应用研究所 | Paenibacillus and application thereof to environmental engineering |
| CN102719482A (en) * | 2012-02-28 | 2012-10-10 | 湖南大学 | Method for producing microbial flocculant by utilizing culture wastewater and residual activated sludge |
| CN103451232A (en) * | 2012-05-28 | 2013-12-18 | 浙江树人大学 | Preparation method and use method of self-circulation microbial flocculant |
| CN102925489A (en) * | 2012-10-10 | 2013-02-13 | 临安天川环保科技有限公司 | Preparation method of microbial flocculant |
| CN102992457A (en) * | 2012-11-25 | 2013-03-27 | 溧阳市天目湖保健品有限公司 | Method for producing coagulant aid through utilizing cow dung |
| CN103184239A (en) * | 2013-03-11 | 2013-07-03 | 宁波飞日水产实业有限公司 | Method for producing microbial flocculant by using surimi processing waste water |
| CN105296400A (en) * | 2015-11-26 | 2016-02-03 | 南京贝克特环保科技有限公司 | Microbial nutrient for biological coagulation treatment of feces in pig breeding farm and production process of microbial nutrient |
| CN105296401A (en) * | 2015-11-26 | 2016-02-03 | 南京贝克特环保科技有限公司 | Biological coagulation treatment microorganism nutritional agent for feces in dairy farm and production process of nutritional agent |
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Open date: 20100310 |