CN101647369A - New process for producing three-level strain of edible fungus - Google Patents
New process for producing three-level strain of edible fungus Download PDFInfo
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- CN101647369A CN101647369A CN200910070469A CN200910070469A CN101647369A CN 101647369 A CN101647369 A CN 101647369A CN 200910070469 A CN200910070469 A CN 200910070469A CN 200910070469 A CN200910070469 A CN 200910070469A CN 101647369 A CN101647369 A CN 101647369A
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Abstract
The invention relates to a method for effectively preparing a three-level strain of an edible fungus, which comprises the following steps: mixing culture substrates according to a certain proportion,sterilizing, vaccinating and respectively packaging in culture containers; culturing; and obtaining finished products. The invention simplifies the process, saves a plurality of manpower, shortens theproduction period of the strain by mixing vaccination, reduces the probability of infecting mixed bacteria and realizes the aims of rapidly and efficiently manufacturing the strain of the edible fungus.
Description
Technical field
What the present invention relates to is a kind of method of cultivating the breeding edible mushroom mycelium by sterile working in a large number.
Background technology
In the edible mushroom actual production, source, reproductive order of generation and production purpose according to bacterial classification are divided into first class inoculum, second class inoculum and three-class strain to bacterial classification.Edible fungus species production is to finish by the order of first class inoculum, second class inoculum, three-class strain.First class inoculum generally is the pure culture that adopts spore separation method or tissue isolation to obtain, and moves to be connected on and cultivates form purebred on the test tube slant medium.First class inoculum expands numerous on slant medium once more, and resulting bacterial classification is the regeneration first class inoculum.Second class inoculum is moved in the seed bottle of receiving solid culture mediums such as wood chip or excrement grass are housed by first class inoculum or regeneration first class inoculum, cultivates through thermophilic to form.Three-class strain is directly used in the inoculation of Duan Mu, bacterium bed or cultivation bag, and through cultivating the production fruit body.This bacterial classification production process of three types comprises: obtain the mixed preparing of culture materials → culture matrix → be sub-packed in the culture vessel → sterilization → inoculation → cultivation → check → use.
Above current technology is owing to after the pack sterilization earlier, inoculate, so need great amount of manpower to go to finish, so at first being to have increased human cost, secondly is the probability that has increased bacteria infection, and the 3rd is to inoculate point very little, cause the bacterial classification production cycle to prolong, technology is also very complicated in addition.And the purpose of this invention is to provide a kind of effective ways of making edible fungus species fast, efficiently.
Summary of the invention
The purpose of this invention is to provide a kind of effective ways of making edible fungi three-class strain fast, efficiently.Be to sterilize after the culture matrix mixed preparing earlier, be sub-packed in the cultivating container again after the inoculation and cultivate.
Technical scheme of the present invention is: a kind of new process for producing three-level strain of edible fungus, it is characterized in that: with culture matrix by a certain percentage after the mixed preparing by sterilize → inoculate → be sub-packed in the culture vessel → production process of cultivation → finished product, obtain qualified edible fungus species.
Described edible fungus species production new technique, culture matrix wherein is mixed processing by a certain percentage, accurately takes by weighing according to the composts or fertilisers of cultivating prescription of different edible fungus culturing kinds, and different material is carried out preliminary treatment according to distinct methods, as use straw, then be cut into segment earlier and add water and prewet.For the slightly solubility material, should be even with the major ingredient dry mixing before adding water; For the ease of solubility material, should dissolve by first water, admix in the material with water in proportion then.The water content of composts or fertilisers of cultivating is decided according to the biological property of raw material type, physical behavior and mushroom class, generally between 55%-65%.After mixing thoroughly stockpile is amassed, vexed 30-60 minute, make it have thorough grasp moisture, regulating the pH value is 6.5-8.
Described edible fungus species production new technique; its sterilizing methods is: the composts or fertilisers of cultivating of mixing thoroughly after will mixing is packed in the closed container; the charging volume accounts for 2/3 of vessel volume; can not compacting; carry out the atmospheric steam sterilization, reach after 100 ℃, kept 8-12 hour in temperature; stewing again the steaming 2-3 hour still is transported to sterile workshop with composts or fertilisers of cultivating after the cooling after the truce under air-tight state.
Described edible fungus species production new technique, its inoculation method is: second class inoculum is blended into particle about 0.5 centimetre, pour in the cooled composts or fertilisers of cultivating mixing under air-tight state according to the ratio of weight ratio 1: 10-20 fast into.
Described edible fungus species production new technique, its method for filling is: bacterium material environment should be gnotobasis.Before the dress bacterium material, culture vessel also will carry out sterilization treatment.Plastic sack generally adopts polyethylene or polypropylene material, uses formaldehyde 8-10mL/m in confined space in advance
3+ KMn0
44-5g/m
3Stifling 0.5-1 hour, use ultra violet lamp again.When filling with substance, the bacterium bag is enclosed within on the pipeline of carrying the bacterium material, by auger the bacterium material is sent in the bacterium bag, pack then installs to suitable tying and is advisable, preferred bacterium material is filled to from sack 8-10cm, cover neck ring, plug tampon, usually, wood chip, cotton seed hulls, corn cob granule etc. want degree of tightness suitable, the compacting of will exerting oneself of rice (wheat) grass meal.Installing the back seals with tampon.
Described edible fungus species production new technique, its cultural method is: the bacterium that branch installs is expected that putting into culturing room sends out bacterium.Culturing room answers disinfection before use, and door and window can be airtight, dark, and unlatching can guarantee that room ventilation is good.When putting bacterial classification, should leave a fixed gap, and running check.Temperature is controlled at 22-23 ℃, and relative moisture is controlled at 60%-70%, and often ventilation was generally covered with at 15-25 days, finished cultivation, and it covered with in advance than the routine inoculation in 5-10 days.
Characteristics of the present invention are a kind of edible fungus species production new techniques.By current technology is adjusted improvement, make it become first sterilization, inoculate, technology has been simplified in pack at last, has saved great amount of manpower, because it adopts combined inoculation, the bacterial classification production cycle is shortened, reduced the probability of bacteria infection, realized making fast, efficiently the purpose of edible fungus species.
Specific embodiment one:
The flat mushroom material formula is: 83 kilograms of cotton seed hulls, 15 kilograms of wheat brans, 2 kilograms of lime, 120 kg water.Siccative is added the spice machine, and premix 2 minutes adds entry 120kg then; stirred 5 minutes, and behind the spice mixing, piled up 50 minutes; be sent in the sterilizer again; charge accounts for 2/3 of container volume, by introducing vapours heating composts or fertilisers of cultivating temperature to 100 ℃, keep pressure 12h after; stewing again the steaming 3 hours after the truce; the introducing cooling water enters sleeve and carries out outer cooling, when being cooled to the composts or fertilisers of cultivating central temperature below 25 ℃, prepares inoculation.
Bacterial classification is handled: 15 kilograms of flat mushroom original seeds are placed in the desinfection chamber, draw bottle, pulverize original seed to the 0.5cm size particles, place in the inoculation container.
Inoculation: the bacterial classification that will handle inserts composts or fertilisers of cultivating by the bacterial classification container, behind the stirring and evenly mixing, to desinfection chamber, carries out direct can by screw rod transmission.
Can: the plastic sack of the bacterium of going out that will be more bigger than discharging opening bore, be enclosed within discharging opening, control the elasticity of can by the squeezing action of screw rod generation.Size control can amount according to plastic sack.Overlap neck ring, plug tampon then, send into thermostatic chamber and send out bacterium.Temperature is controlled at 22-23 ℃, and relative moisture is controlled at about 65%, often ventilation.
After can finished, hot water injection's sterilizer and pipeline did not flow out to there being obvious impurity, and wet sterilization 15 minutes utilizes waste heat oven dry or wet sterilization 30 minutes, and charging is recycling once more.
Attention: in bacterial classification processing, inoculation and pouring process, all need to operate by the sterile working requirement.
Implement that the bacterial classification of gained has following index after the above-mentioned technological process:
1. bacterium material color transition is a dark brown;
2. bacterium material water content is about 65%;
3.pH about 7.0;
4. culture matrix does not have assorted bacterium;
5. bacterial classification and composts or fertilisers of cultivating mix;
6. to send out bacterium fast for bacterium rod, can send out in about 15～20 days bacterium full;
7. whole bacterium rod cell age is more consistent, and regularity is good.
Specific embodiment two:
The mushroom materials prescription is: 78 kilograms of cotton seed hulls, 20 kilograms of wheat brans, 1 kilogram of gypsum, 1 kilogram of calcium carbonate, 115 kg water.Solid material is packed in the spice machine, and valve-off carried out the raw material premix 5 minutes; add entry then, stirred 10 minutes, behind the mixing; piled up 40 minutes; be sent in the sterilizer, vapours heating composts or fertilisers of cultivating temperature to 100 ℃ is kept pressure 12h again; stewing again the steaming 3 hours after the truce; introduce cooling water and carry out tube-cooled, when being cooled to the composts or fertilisers of cultivating central temperature below 25 ℃, inoculate.
Bacterial classification is handled: 20 kilograms of mushroom original seeds are placed in the desinfection chamber, draw bottle, the bacterium rod is crushed to the 0.5cm size particles, places in the inoculation container.
Inoculation: the bacterial classification that will handle inserts cultivate material by the bacterial classification container, stirs 10 minutes mixings, arrives desinfection chamber by screw rod transmission behind the mixing, carries out direct can.
Can: plastic sack that will be more bigger than discharging opening bore, be enclosed within after the sterilization on the discharging opening, control the elasticity of can by the squeezing action of screw rod generation.Size control can amount according to plastic sack.General bacterium material is filled to from sack less than 10cm, and cover neck ring, plug tampon are sent into thermostatic chamber and sent out bacterium.Temperature is controlled at 22-23 ℃, and relative moisture is controlled at about 65%, often ventilation.
After can finished, hot water injection's sterilizer, inoculator and conveyance conduit did not flow out to there being obvious impurity, and wet sterilization 15 minutes utilizes waste heat oven dry or wet sterilization 30 minutes, and charging is recycling once more.
Implement that the cultivation strain of gained has following index after the above-mentioned technological process:
1. bacterium material color transition is a dark brown;
2. bacterium material water content is about 60%;
3.pH about 6.0;
4. culture matrix does not have assorted bacterium;
5. bacterial classification and composts or fertilisers of cultivating mix;
6. to send out bacterium fast for bacterium rod, can send out in about 20～25 days bacterium full;
7. whole bacterium rod cell age is consistent, the regularity height.
Advantage of the present invention is:
The present invention makes it become earlier sterilization by current technology is adjusted improvement, inoculates, Technology has been simplified in rear pack, has saved a large amount of manpowers, because it adopts combined inoculation, makes bacterial classification Production cycle shortens, and has reduced the probability of bacteria infection, has realized making fast, efficiently edible mushroom The purpose of bacterial classification. Institute's adopting process meets current environmental protection, efficient, energy-conservation principle, and its benefit is expected Be doubled and redoubled, can obtain good economic results in society.
Claims (6)
1. a new process for producing three-level strain of edible fungus is characterized in that: culture matrix is passed through sterilization after the mixed preparing by a certain percentage, inoculate, be sub-packed in the culture vessel, cultivate, obtain qualified edible fungus species.
2. a kind of new process for producing three-level strain of edible fungus according to claim 1, it is characterized in that: with culture matrix mixed processing by a certain percentage, composts or fertilisers of cultivating prescription according to different edible fungus culturing kinds accurately takes by weighing, and different material carried out preliminary treatment according to distinct methods, the water content of composts or fertilisers of cultivating after mixing thoroughly is amassed stockpile between 55%-65%, vexed 30-60 minute, make it have thorough grasp moisture, regulating the pH value is 6.5-8.
3. a kind of new process for producing three-level strain of edible fungus according to claim 1; it is characterized in that: the composts or fertilisers of cultivating of mixing thoroughly after will mixing is packed in the closed container; the charging volume accounts for 2/3 of vessel volume; can not compacting; carry out the atmospheric steam sterilization, reach after 100 ℃, kept 8-12 hour in temperature; stewing again the steaming 2-3 hour still is transported to sterile workshop with composts or fertilisers of cultivating after the cooling after the truce under air-tight state.
4. a kind of new process for producing three-level strain of edible fungus according to claim 1, it is characterized in that: second class inoculum is blended into particle about 0.5 centimetre, pour in the cooled composts or fertilisers of cultivating mixing under air-tight state fast into according to the ratio of weight ratio 1: 10-20.
5. a kind of new process for producing three-level strain of edible fungus according to claim 1, it is characterized in that: the charging environment is aseptic, and before the dress bacterium material, culture vessel carries out sterilization treatment, plastic sack generally adopts polyethylene or polypropylene material, uses formaldehyde 8-10mL/m in confined space in advance
3+ K
2MnO
44-5g/m
3Stifling 0.5-1 hour, use ultra violet lamp again, when filling with substance, the bacterium material is filled to from sack 8-10cm, cover neck ring, plug tampon, wood chip, cotton seed hulls, corn cob granule etc. want degree of tightness to suit, the compacting of will exerting oneself of rice or barley seeding food, install the back and seal, the bacterium that branch installs is expected that putting into culturing room sends out bacterium with tampon.
6. a kind of new process for producing three-level strain of edible fungus according to claim 1, it is characterized in that: culturing room answers disinfection before use, and door and window can be airtight, dark, and unlatching can guarantee that room ventilation is good, when putting bacterial classification, should leave a fixed gap, and running check, temperature is controlled at 22-23 ℃, relative moisture is controlled at 60%-70%, often ventilation was generally covered with at 15-25 days, finished cultivation.
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| CN200910070469A CN101647369A (en) | 2009-09-17 | 2009-09-17 | New process for producing three-level strain of edible fungus |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102047813A (en) * | 2010-10-20 | 2011-05-11 | 天津渔阳酒业有限责任公司 | Sterilization and inoculation process for edible fungus culture material |
| CN103609332A (en) * | 2013-11-19 | 2014-03-05 | 福建容益菌业科技研发有限公司 | Loading method for industrial cultivation of Sparassis crispa |
| CN105325177A (en) * | 2015-11-27 | 2016-02-17 | 李军 | Efficient edible mushroom planting technology |
| CN105557311A (en) * | 2016-01-11 | 2016-05-11 | 丽水市农业科学研究院 | Method for making edible fungus stick |
| CN106973697A (en) * | 2017-04-11 | 2017-07-25 | 宁德师范学院 | A kind of corn pellet edible mushroom immobilized bacteria production and cultural method |
| WO2017219394A1 (en) * | 2016-06-24 | 2017-12-28 | 四川省大真科技有限责任公司 | Seed production method of cultivated species of edible fungus and cultivation method of cultivated species and edible fungus prepared thereby |
| CN111670755A (en) * | 2020-07-29 | 2020-09-18 | 平泉市瀑河源食品有限公司 | Edible fungus inoculation process for solid fungus |
| CN111670754A (en) * | 2020-07-29 | 2020-09-18 | 平泉市瀑河源食品有限公司 | Edible fungus inoculation process for liquid fungus |
-
2009
- 2009-09-17 CN CN200910070469A patent/CN101647369A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102047813A (en) * | 2010-10-20 | 2011-05-11 | 天津渔阳酒业有限责任公司 | Sterilization and inoculation process for edible fungus culture material |
| CN103609332A (en) * | 2013-11-19 | 2014-03-05 | 福建容益菌业科技研发有限公司 | Loading method for industrial cultivation of Sparassis crispa |
| CN105325177A (en) * | 2015-11-27 | 2016-02-17 | 李军 | Efficient edible mushroom planting technology |
| CN105557311A (en) * | 2016-01-11 | 2016-05-11 | 丽水市农业科学研究院 | Method for making edible fungus stick |
| WO2017219394A1 (en) * | 2016-06-24 | 2017-12-28 | 四川省大真科技有限责任公司 | Seed production method of cultivated species of edible fungus and cultivation method of cultivated species and edible fungus prepared thereby |
| CN106973697A (en) * | 2017-04-11 | 2017-07-25 | 宁德师范学院 | A kind of corn pellet edible mushroom immobilized bacteria production and cultural method |
| CN111670755A (en) * | 2020-07-29 | 2020-09-18 | 平泉市瀑河源食品有限公司 | Edible fungus inoculation process for solid fungus |
| CN111670754A (en) * | 2020-07-29 | 2020-09-18 | 平泉市瀑河源食品有限公司 | Edible fungus inoculation process for liquid fungus |
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Open date: 20100217 |