CN101642189A - Microcapsule compound feed additive and preparation method thereof - Google Patents
Microcapsule compound feed additive and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a microcapsule compound feed additive and a preparation method thereof, and belongs to the field of feed additives. The microcapsule compound feed additive comprises a microcapsule lactobacillus preparation, microcapsule eucommia bark extract, and a carrier. The microcapsule compound feed additive has the effects of the lactobacillus preparation and the eucommia bark extract, makes the two exert synergia, can highly effectively promote the multiplication of endogenous and ectogenic beneficial bacteria in intestinal canal, balance a microbial eco-system in the ntestinalcanal, inhibit the growth and reproduction of harmful bacteria, improve immunity of organs of animals, ensure the health of the intestinal canal of the animals, reduce the disease rate of the intestinal canal of the animals, promote the growth of the animals and improve feed conversion rate, and is suitable for developing green and healthy breeding and producing non-polluted animal products.
Description
Technical field
The present invention relates to a kind of microcapsule compound feed additive and preparation method thereof, belong to feed additive field.
Background technology
Feed addictive especially trophic function forage additive is the core of complete feed, concentrate feed, premix.Microcapsules technology is with special method solid, liquid or gaseous matter embedding to be sealed up for safekeeping at a kind of microencapsulation third to become the solia particle product, when needed the technology that will be discharged by the content of embedding again.Lactic acid bacteria is carried out microencapsulation, the poor environment of thalline with the external world can be separated, avoid the infringement of trace element, the influence that slows down temperature and pressure in the pelletization; Form solid particle, be beneficial to the even distribution in premix, also help storing and transportation; After adopting enteric solubility wall material, can also prevent the destruction of gastric juice, thereby make thalline as much as possible arrive enteron aisle, really play the effect of health care and treatment.
Bark of eucommia Eucommia ulmoides Oliv. is an Eucommiaceae bark of eucommia platymiscium, is China's special product.The existing research report of the chemical composition of the bark of eucommia, from folium cortex eucommiae, mainly be separated to 6 flavone compounds, be respectively kaempferol (Kaempferol), Quercetin (Quercetin), astragalin (Astragalin), land brocade (Hirsu-tin), rutin (Rutin) and hyperin (Hypefin), it is directly used in functional health raises, have broad spectrum antibacterial performance and the effect that improves the immune immunocompetence of animal body, add in the animal feed and can improve food conversion ratio as feed addictive, play the effect of disease-resistant growth-promoting.But because Flavonoid substances mostly is pure soluble substance, the chance shipwreck is molten, and strong bitter taste is arranged, and directly adds the mouthfeel that influences feed in the feed to, and serious meeting causes the animal apocleisis.
At present, the research of feeding lactobacillus microcapsules technology in China's feed industry is used and still is in the starting stage, also exist such as: the viable lactic acid bacteria content of microencapsulation is low, the storage life is short, the wall material is difficult for shortcomings such as dissolving in enteron aisle, therefore develops the viable bacteria content height, stablizes, the lactobacillus micro-capsule product of enteric solubility is applied to have great practical significance and commercial value in the feed addictive.Eucommia ulmoides extracts is little as feed addictive green, toxic and side effect, its microencapsulation can be improved the local flavor and the mouthfeel of animal products, and lactobacillus feed additive and eucommia ulmoides extracts compound the two have the effect of Synergistic, can improve the animal body immunocompetence, stimulate the growth of animal intestinal endogenous and exogenous lactic bacteria, strengthen the metabolic activity of lactic acid bacteria, effect such as improve food conversion ratio.But lactobacillus micro-capsule and the compound feed addictive of making of eucommia ulmoides extracts microcapsules are not appeared in the newspapers.
Summary of the invention
The purpose of this invention is to provide and a kind ofly can improve animal body immunity, improve food conversion ratio, good stability, safety, the green feed additive of animal happiness food, this additive is with the microencapsulation lactobacillus preparation, the effect of eucommia ulmoides extracts, and the two Synergistic, can either efficiently promote the propagation of enteron aisle endogenous and exogenous beneficial bacterium, balance intestinal microecology environment suppresses the growth and breeding of harmful bacteria, guarantee the intestinal health of animal, reduce the incidence of disease of animal intestinal, can promote growth of animal again, and be suitable for developing green health and culture, produce the no public nuisance livestock product.
Another object of the present invention provides the preparation method of this microcapsule compound feed additive.
The objective of the invention is to be achieved through the following technical solutions:
The invention provides a kind of microcapsule compound feed additive, it comprises microencapsulation lactobacillus preparation, microencapsulation eucommia ulmoides extracts, carrier, and the weight proportion of mentioned component is: 1: 0.1～1: 1～10.
Wherein, described microencapsulation lactobacillus preparation can comprise other lactobacillus micro-capsule, but the present invention preferably comprises the mixture microcapsules of enterococcus faecalis microcapsules, lactobacillus acidophilus microcapsules, Lactobacillus plantarum, and the weight proportion of mentioned component is: 1: 0.5～3: 0.5～3.
Above-mentioned enterococcus faecalis microcapsules are enterococcus faecalis CGMCC No.2385 microcapsules and/or enterococcus faecalis CGMCCNo.2386 microcapsules, enterococcus faecalis CGMCC No.2385 separates from healthy animal enteron aisle or ight soil for the applicant with enterococcus faecalis CGMCC No.2386, seed selection obtains, identify through Chinese agriculture microorganism fungus kind preservation administrative center, and be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation (being called for short CGMCC) on March 3rd, 2008.Two strain bacterial classifications have anti-adversity such as good stomach juice-resistant, bile tolerance, high temperature resistant, tolerance common antibiotics and produce acid, produce enzyme, suppress benefit such as pathogen and give birth to functions, and contents such as the separation of bacterial classification, selection and characteristic thereof can be referring to the Chinese patent that among the applicant please number be 200810106520.5; Lactobacillus acidophilus and Lactobacillus plantarum can be bought by market and obtain.
In the microcapsule compound feed additive of the present invention used carrier be in glucose, rice bran, the stone flour 1,001 kinds, two or more.
The present invention also provides the preparation method of this microcapsule compound feed additive, and its step comprises:
1. prepare microencapsulation lactobacillus preparation and microencapsulation eucommia ulmoides extracts respectively;
2. in proportion microencapsulation lactobacillus preparation, microencapsulation eucommia ulmoides extracts, carrier are mixed, make microcapsule compound feed additive.
Wherein, preparation microencapsulation lactobacillus preparation comprises following steps:
1. the preparation of wall material:
A, wall material are formed: natural plant gum, and protein and starchy material, the weight proportion of mentioned component are 1: 0.5～2: 1～5;
B, the wall material is mixed with 5～15% solution, is heated to 50～70 ℃, stir, make its dissolving, be cooled to 35～45 ℃;
2. lactic acid bacteria bacterium mud is joined in the wall material solution, bacterium mud and wall material solution weight volume ratio are between 1: 5～1: 10, rotating speed 1000～4000rpm, even matter emulsification;
3. emulsion spray drying, 160～180 ℃ of inlet temperatures, 70～90 ℃ of outlet temperatures, atomizer rotating speed 20000rpm obtains the microencapsulation lactobacillus preparation.
Described natural plant gum is Arabic gum or agar; Described protein is any in zein, soybean protein, the skimmed milk power; Described starchy material is modified starch and/or maltodextrin.
Wherein, the preparation method of bacterium mud comprises the steps:
The preparation method of A, enterococcus faecalis bacterium mud
1. dull and stereotyped cultivation rejuvenation: the enterococcus faecalis bacterial classification inoculation on the MRS plating medium, in 32～37 ℃ of cultivation 12～24h, is made the enterococcus faecalis rejuvenation, and forms single bacterium colony; Picking list colony inoculation is cultivated 24～36h in 32～37 ℃ on slant medium;
2. the preparation of first order seed: the enterococcus faecalis slant strains that 1. step is cultivated is transferred in the 500mL triangular flask that 250ml～300ml MRS culture medium is housed, cultivate 12～16h in 32～37 ℃, rotating speed 100～150rpm makes it be in the logarithm middle and later periods, is first order seed;
3. the preparation of secondary seed: the Bacillus acidi lactici first order seed that 2. step is cultivated is transferred in the 2.0L triangular flask that 1.2～1.6L MRS culture medium is housed, and in 32～37 ℃ of static cultivation 12～16h, is secondary seed solution;
4. preparation of fermentation liquid: the secondary seed solution that 3. step is prepared is inoculated into according to 3～6% inoculum concentration 12～16M is housed
3In the fermentation tank of fermentation medium, 35～37 ℃ of temperature, rotating speed 120～160rpm, tank pressure 0.05MPa cultivates 16～20h, to viable count be 2～5 * 10
9Cfu/ml, stuck fermentation;
Described fermentation medium is: glucose 1～2%, soy peptone 1～2%, ammonium sulfate 0.5～1.0%, sodium chloride 0.2～0.8%, magnesium sulfate 0.01～0.05%;
5. centrifugal concentrate: zymotic fluid is centrifugal, and rotating speed is 16000～20000rpm, and charging rate is 0.5～1kg/min, to water content be 75～95% o'clock, stop centrifugal, bacterium mud.
The preparation method of B, lactobacillus acidophilus, Lactobacillus plantarum bacterium mud
1. one-level is cultivated: adopt conventional MRS culture medium as slant medium, preparation one-level kind the inclined-plane, 37 ℃, cultivated 24～30 hours;
2. secondary Liquid Culture: use the MRS culture medium, under 37 ℃, pH4.5～6.0,150～180rpm shaken cultivation 16～18 hours;
3. preparation of fermentation liquid: adopt the used fermentation medium of secondary Liquid Culture, in fermentation tank, cultivate, inoculum concentration is 0.5～3%, liquid amount 60～80%, rotating speed 80～120rpm, ventilation ratio 1: 0.2～1: 0.5,37 ℃ of pH4.5～5.0 of temperature, cultivated 18～24 hours, and got zymotic fluid;
Described fermentation medium is: glucose 1～5%, yeast extract 0.05～3%, soy peptone
Ammonium sulfate 0.05～4%, calcium carbonate 0.2～5%.
5. centrifugal concentrate: zymotic fluid is undertaken centrifugal by tube centrifuge, rotating speed is 16000～2000rpm, and charging rate is 0.5～1kg/min, to water content be 75～95% o'clock, stop centrifugal, bacterium mud.
The microencapsulation eucommia ulmoides extracts comprises following steps among the preparation method of microcapsule compound feed additive of the present invention:
1. the preparation of wall material:
A, wall material are formed: natural plant gum and starchy material, the weight proportion of mentioned component are 1: 0.5～2: 1～5;
B, the wall material is mixed with 10～20% solution, is heated to 50～70 ℃, stir, make its dissolving, be cooled to 35～45 ℃;
2. eucommia ulmoides extracts is joined in the wall material solution, the w/v of eucommia ulmoides extracts and wall material solution is 1: 5～1: 10, rotating speed 1000～4000rpm, even matter emulsification;
3. emulsion spray drying, 160～180 ℃ of inlet temperatures, 70～90 ℃ of outlet temperatures, atomizer rotating speed 20000rpm obtains the microencapsulation eucommia ulmoides extracts.
Used natural plant gum is Arabic gum or agar in the microencapsulation eucommia ulmoides extracts process; Used starchy material is modified starch and/or maltodextrin.
Microcapsule compound feed additive of the present invention is with the effect of lactobacillus preparation, eucommia ulmoides extracts, and the two Synergistic, can either efficiently promote the propagation of enteron aisle endogenous and exogenous beneficial bacterium, balance intestinal microecology environment suppresses the growth and breeding of harmful bacteria, can improve animal body immunity again, guarantee the intestinal health of animal, reduce the incidence of disease of animal intestinal; Simultaneously, this feed additive animal happiness food can promote growth of animal, improves food conversion ratio, and is suitable for developing green health and cultures, and produces the no public nuisance livestock product.
, it should be understood that described embodiment only is for the present invention is described, rather than limit the scope of the invention by any way more specific description the present invention by the following example.
The specific embodiment
The preparation of embodiment 1 lactobacillus micro-capsule
The preparation of A enterococcus faecalis microcapsules:
1. the wall material is formed: weight ratio: Arabic gum: zein: modified starch=1: 0.5: 1 mixes;
With the wall material form be mixed with 15% solution, be heated to 70 ℃, stir, make its dissolving, be cooled to 35～45 ℃;
2. enterococcus faecalis bacterium mud joins in the above-mentioned wall material solution, and the w/v of bacterium mud and wall material solution is 1: 5, and promptly 1kg bacterium mud joins in the 5L wall material solution, even matter emulsification, and rotating speed 2000rpm obtains emulsion;
3. emulsion spray drying, 160 ℃ of inlet temperatures, 70 ℃ of outlet temperatures, atomizer rotating speed 20000rpm obtains the enterococcus faecalis microcapsule formulation, and the microcapsules viable count is 2 * 10
10CFU/g, micro-capsule coating rate 81%.
Wherein, the preparation method of enterococcus faecalis bacterium mud is as follows:
1. dull and stereotyped cultivation rejuvenation: the enterococcus faecalis bacterial classification inoculation on the MRS plating medium, in 37 ℃ of cultivation 12h, is made the rejuvenation of enterococcus faecalis bacterium, and forms single bacterium colony; Picking list colony inoculation is cultivated 36h in 37 ℃ on slant medium;
2. the preparation of first order seed: the enterococcus faecalis slant strains that 1. step is cultivated is transferred in the 500mL triangular flask that 300ml MRS culture medium is housed, and in 37 ℃ of cultivation 16h, rotating speed 150rpm makes it be in the logarithm middle and later periods, is first order seed;
3. the preparation of secondary seed: the Bacillus acidi lactici first order seed that 2. step is cultivated is transferred in the 20L triangular flask that 1.6L MRS culture medium is housed, and in 37 ℃ of static cultivation 16h, is secondary seed solution;
4. preparation of fermentation liquid: the secondary seed solution that 3. step is prepared is inoculated into according to 6% inoculum concentration 16M is housed
3In the fermentation tank of fermentation medium, 35～37 ℃ of temperature, rotating speed 120rpm, tank pressure 0.05MPa cultivates 18h, to viable count be 5 * 10
9Cfu/ml, stuck fermentation;
Fermentation medium is: glucose 2%, soy peptone 2%, ammonium sulfate 1.0%, sodium chloride 0.8%, magnesium sulfate～0.05%;
5. centrifugal concentrate: zymotic fluid is undertaken centrifugal by tube centrifuge, rotating speed is 16000rpm, and charging rate is 0.5kg/min, to water content be 75% o'clock, stop centrifugal, bacterium mud, water content 75%;
The preparation of B lactobacillus acidophilus microcapsules:
1. the wall material is formed: weight ratio: Arabic gum: soybean protein: maltodextrin=1: 1: 2.5 mixes;
With the wall material form be mixed with 10% solution, be heated to 50 ℃, stir, make its dissolving, be cooled to 35 ℃;
2. lactobacillus acidophilus bacterium mud joins in the above-mentioned wall material solution, and the w/v of bacterium mud and wall material solution is 1: 7.5, and promptly 1kg bacterium mud joins in the 7.5L wall material solution, even matter emulsification, and rotating speed 1000rpm obtains emulsion;
3. emulsion spray drying, 170 ℃ of inlet temperatures, 80 ℃ of outlet temperatures, atomizer rotating speed 20000rpm obtains the lactobacillus acidophilus microcapsule formulation, and the microcapsules viable count is 2.5 * 10
10CFU/g, micro-capsule coating rate 85%;
Wherein, being prepared as follows of lactobacillus acidophilus bacterium mud:
1. one-level is cultivated: adopt conventional MRS culture medium as slant medium, preparation first order seed inclined-plane, was cultivated 24 hours by 37 ℃;
2. secondary Liquid Culture: use the MRS culture medium, under 37 ℃, pH4.5～6.0,150rpm shaken cultivation 18 hours;
3. preparation of fermentation liquid: adopt the used fermentation medium of secondary Liquid Culture, cultivate in fermentation tank, inoculum concentration is 3%, liquid amount 80%, rotating speed 120rpm, ventilation ratio 1: 0.5,37 ℃ of temperature, pH4.5～5.0, cultivated 18 hours, and got zymotic fluid, viable count 4.0 * 10
9Cfu/ml;
Fermentation medium is: glucose 5%, yeast extract 1.5%, soy peptone 3%, ammonium sulfate 0.05%, calcium carbonate 0.2%;
4. centrifugal concentrate: zymotic fluid is undertaken centrifugal by tube centrifuge, rotating speed is 20000rpm, and charging rate is 1kg/min, obtains bacterium mud, water content 80%.
The preparation of C Lactobacillus plantarum microcapsules
1. the wall material is formed: weight ratio: agar powder: skimmed milk power: maltodextrin=1: 2: 5 mixes;
The wall material formed be mixed with 20% solution, be heated to 60 ℃, stir, make its dissolving, be cooled to 40 ℃;
2. Lactobacillus plantarum bacterium mud joins in the above-mentioned wall material solution, and the w/v of bacterium mud and wall material solution is 1: 10, and promptly 1kg bacterium mud joins in the 10L wall material solution, even matter emulsification, and rotating speed 4000rpm obtains emulsion;
3. emulsion spray drying, 170 ℃ of inlet temperatures, 80 ℃ of outlet temperatures, atomizer rotating speed 20000rpm obtains the Lactobacillus plantarum microcapsule formulation, and the microcapsules viable count is 2.2 * 10
10CFU/g, micro-capsule coating rate 80%;
Wherein, Lactobacillus plantarum bacterium mud is prepared as follows
1. one-level is cultivated: adopt conventional MRS culture medium as slant medium, preparation first order seed inclined-plane, was cultivated 24 hours by 37 ℃;
2. secondary Liquid Culture: use the MRS culture medium, under 37 ℃, pH4.5～6.0,180rpm shaken cultivation 18 hours;
3. preparation of fermentation liquid: fermentation medium is: glucose 5%, and yeast extract 3%, soy peptone 3% is dredged sour ammonium 4%, calcium carbonate 5%;
Adopt the used fermentation medium of secondary Liquid Culture, cultivate in fermentation tank, inoculum concentration is 3%, liquid amount 60%, and rotating speed 120rpm, ventilation ratio 1: 0.5,37 ℃ of temperature, cultivated 18 hours pH4.5～5.0, gets zymotic fluid, viable count 4 * 10
9Cfu/ml.
4. centrifugal concentrate: zymotic fluid is undertaken centrifugal by tube centrifuge, rotating speed is 20000rpm, and charging rate is 1kg/min, bacterium mud, water content is 85%.
The preparation of embodiment 2 eucommia ulmoides extracts microcapsules
1. the wall material is formed: Arabic gum, modified starch weight ratio: 1: 1
The wall material is mixed with 10% solution, is heated to 70 ℃, stirs, and makes its dissolving, is cooled to 45 ℃;
2. eucommia ulmoides extracts is joined in the wall material solution, eucommia ulmoides extracts and wall material solution weight volume ratio are 1: 5, even matter emulsification, rotating speed 1000rpm;
3. emulsion spray drying, 180 ℃ of inlet temperatures, 90 ℃ of outlet temperatures, atomizer rotating speed 20000rpm obtains bark of eucommia microcapsule formulation.
The preparation of embodiment 3 eucommia ulmoides extracts microcapsules
1. the wall material is formed: agar, maltodextrin weight ratio: 1: 2.5
The wall material is mixed with 20% solution, is heated to 60 ℃, stirs, and makes its dissolving, is cooled to 40 ℃;
2. eucommia ulmoides extracts is joined in the wall material solution, eucommia ulmoides extracts and wall material solution weight volume ratio are 1: 7.5, even matter emulsification, rotating speed 2000rpm;
3. emulsion spray drying, 170 ℃ of inlet temperatures, 70 ℃ of outlet temperatures, atomizer rotating speed 2000rpm obtains bark of eucommia microcapsule formulation.
The preparation of embodiment 4 eucommia ulmoides extracts microcapsules
1. the wall material is formed: Arabic gum, maltodextrin weight ratio: 1: 5;
The wall material is mixed with 15% solution, is heated to 50 ℃, stirs, and makes its dissolving, is cooled to 35 ℃;
2. eucommia ulmoides extracts is joined in the wall material solution, eucommia ulmoides extracts and wall material solution weight volume ratio are 1: 10, even matter emulsification, rotating speed 4000rpm;
3. emulsion spray drying, 160 ℃ of inlet temperatures, 80 ℃ of outlet temperatures, atomizer rotating speed 20000rpm obtains bark of eucommia microcapsule formulation.
The preparation of embodiment 5 microcapsule compound feed additives
1) microencapsulation enterococcus faecalis, microencapsulation lactobacillus acidophilus, the microencapsulation Lactobacillus plantarum with embodiment 1 preparation mixes with 1: 0.5: 0.5 ratio of weight ratio, gets the microencapsulation lactobacillus preparation;
2) with the microencapsulation eucommia ulmoides extracts of microencapsulation lactobacillus preparation, embodiment 2 preparations, glucose with weight proportion: 1: 1: 5 ratio mixes, and promptly obtains microcapsule compound feed additive, called after W1.
The preparation of embodiment 6 microcapsule compound feed additives
1) microencapsulation enterococcus faecalis, microencapsulation lactobacillus acidophilus, the microencapsulation Lactobacillus plantarum with embodiment 1 preparation mixes with 1: 1.5: 3 ratio of weight ratio, gets the microencapsulation lactobacillus preparation;
2) with the microencapsulation eucommia ulmoides extracts of microencapsulation lactobacillus preparation, embodiment 3 preparations, carrier with weight proportion: 1: 0.1: 5 ratio mixes, and wherein carrier is 1: 1 rice bran and a stone flour, promptly obtains microcapsule compound feed additive, called after W2.
The preparation of embodiment 7 microcapsule compound feed additives
1) microencapsulation enterococcus faecalis, microencapsulation lactobacillus acidophilus, the microencapsulation Lactobacillus plantarum with embodiment 1 preparation mixes with 1: 3: 3 ratio of weight ratio, gets the microencapsulation lactobacillus preparation;
2) with the microencapsulation eucommia ulmoides extracts of microencapsulation lactobacillus preparation, embodiment 4 preparations, carrier with weight proportion: 1: 1: 10 ratio is mixed, wherein carrier is the mixture of rice bran, glucose, stone flour, promptly obtain microcapsule compound feed additive, called after W3.
The efficacy test of embodiment 8 microcapsule compound feed additives
Select for use 1 age in days commodity to mix strong young 360, adopt the design of completely random single-factor to divide 3 groups and test for the Chinese mugwort denapon.Establish 4 repetitions for every group, 30 of every repetitions (beginning body weight group difference is not remarkable, P＞0.05).49 days experimental periods.
The W1 group is for adding the 0.%W1 microcapsule compound feed additive, and the antibiotic control group is for adding 5mg/kg Wei Jiniya mycin, and the blank group is the feeding basal diet only.
Table 1 basal diet is formed (%) and trophic level
| The daily ration constituent | 0～21 age in days | 22～42 ages in days | Behind 43 ages in days |
| Corn | ??57.9 | ??62.3 | ??63.5 |
| Dregs of beans | ??32.0 | ??28.8 | ??28.0 |
| Fish meal | ??3.0 | ??2.0 | ??2.0 |
| Stone flour | ??0.9 | ??0.9 | ??6.9 |
| Calcium monohydrogen phosphate | ??1.4 | ??1.4 | ??1.3 |
| Salt | ??0.3 | ??0.3 | ??0.3 |
| Oil | ??3.5 | ??3.3 | ??3.0 |
| Premix | ??1.0 | ??1.0 | ??1.0 |
| ??ME(MJ/kg) | ??12.47 | ??12.59 | ??12.55 |
| ??CP(％) | ??20.62 | ??19.0 | ??18.78 |
| ??Ca(％) | ??0.97 | ??0.89 | ??0.87 |
| Effective P (%) | ??0.50 | ??0.46 | ??0.44 |
| ??Lys(％) | ??1.05 | ??0.94 | ??0.92 |
| ??Met(％) | ??0.36 | ??0.33 | ??0.33 |
The weight gain of W1 group and the antibiotic control group all utmost point is significantly higher than blank group (P＜0.01), but the W1 group is compared with the antibiotic control group, difference is significantly (P＞0.05) not, illustrate interpolation W1 microcapsules composite feed additive gaining effect and antibiotic suitable, all be better than blank.Each stage gaining effect sees following table for details.
Table 2 is for examination chicken body weight statistical form unit: g
Table 3 material anharmonic ratio, survival rate analytical table
| Group | Full phase weightening finish (g/ only) | Full phase feed consumption (g/ only) | The material anharmonic ratio | Survival rate (%) |
| The blank group | ??1880.88±33.05 | ??4519.75±40.76 | ??2.40±0.04 | ????90.35 |
| The W1 group | ??2073.68±89.83 | ??4595.90±149.60 | ??2.22±0.04 | ????97.65 |
| Antibiosis rope control group | ??2061.92±68.84 | ??4600.56±32.96 | ??2.23±0.06 | ????97.20 |
W1 group and antibiosis rope control group material anharmonic ratio all are starkly lower than blank group (P＜0.01), and the W1 group reduces by 7.50% than blank group; W1 forms motility rate and is significantly higher than blank group (P＜0.01), improves 7.30% than blank group, and is not remarkable with antibiotic control group difference.
The efficacy test of embodiment 9 microcapsule compound feed additives
Selecting body weight for use is 120 of 28 days healthy young milk piglets of (long * big) two-way cross about 7kg, by the principle that body weight is close, hereditary basis is similar, adopts the design of completely random single-factor to divide 4 groups and tests.Establish 3 repetitions (hurdle) for every group, 10 of every repetitions (beginning body weight group difference is not remarkable, p＞0.05), male and female half and half.
W2, W3 are respectively and add 0.1% W2, W3 microcapsule compound feed additive, and the antibiotic control group is for adding golden mould rope (50mg/kg), a blank group feeding basal diet.
Table 4 basal diet (%) and trophic level
| Daily ration is formed | Content (%) | Nutritive index | Trophic level |
| Corn | ????67 | DE(MJ/kg) | ????14.28 |
| The decortication dregs of beans | ????13 | CP(％) | ????16.5 |
| Expanded soybean | ????12 | Ca(％) | ????0.9 |
| Fish meal (60%) | ????4 | P(％) | ????0.7 |
| Premix | ????4 | Lys(％) | ????0.98 |
| Met+Cys(％) | ????0.65 |
The production performance of test piglet sees table 5 for details.Average daily ingestion amount difference is not remarkable between test group; W2, W3 compare with control group: daily gain comparison you can well imagine high 5.5% and 6.6% according to component, and the material anharmonic ratio has descended 5.1% and 3.5%, and diarrhea rate descends 31.1% and 30.3%.The contrast of W2, W3 and antibiotic is compared, and each production performance index differences is not significantly (p＞0.05) all.
Table 5 production performance statistical form
| Group | First counterpoise (kg) | End counterpoise (kg) | Equal daily gain (g/ head) | Average day feed consumption (g/ head) | The material anharmonic ratio | Diarrhea rate (%) |
| ??W2 | ??7.1±0.39 | ??16.6±1.37 | ??316.6 | ??512.9 | ??1.62 | ??9.1±0.7 |
| ??W3 | ??6.9±0.40 | ??16.5±1.42 | ??320.0 | ??527.8 | ??1.65 | ??9.2±0.6 |
| The antibiotic control group | ??7.1±0.42 | ??17.2±1.32 | ??336.5 | ??538.4 | ??1.66 | ??8.8±0.9 |
| The blank group | ??7.0±0.38 | ??16.0±1.51 | ??300.2 | ??513.3 | ??1.71 | ??13.2±1.0 |
Claims (9)
1, a kind of microcapsule compound feed additive is characterized in that comprising microencapsulation lactobacillus preparation, microencapsulation eucommia ulmoides extracts, carrier, and the weight proportion of mentioned component is: 1: 0.1～1: 1～10.
2, microcapsule compound feed additive according to claim 1, it is characterized in that described microencapsulation lactobacillus preparation comprises the mixture microcapsules of enterococcus faecalis microcapsules, lactobacillus acidophilus microcapsules, Lactobacillus plantarum, the weight proportion of mentioned component is: 1: 0.5～3: 0.5～3.
3, microcapsule compound feed additive according to claim 2 is characterized in that described enterococcus faecalis microcapsules are enterococcus faecalis CGMCC No.2385 microcapsules and/or enterococcus faecalis CGMCC No.2386 microcapsules.
4, microcapsule compound feed additive according to claim 1, it is characterized in that described carrier be in glucose, rice bran, the stone flour any, two or more.
5, as the preparation method of each described microcapsule compound feed additive of claim 1-4, its step comprises:
1. prepare microencapsulation lactobacillus preparation and microencapsulation eucommia ulmoides extracts respectively;
2. in proportion microencapsulation lactobacillus preparation, microencapsulation eucommia ulmoides extracts, carrier are mixed, make microcapsule compound feed additive.
6, the preparation method of microcapsule compound feed additive according to claim 5 is characterized in that preparing the microencapsulation lactobacillus preparation and comprises following steps:
1. the preparation of wall material:
A, wall material are formed: natural plant gum, and protein and starchy material, the weight proportion of mentioned component are 1: 0.5～2: 1～5;
B, the wall material is mixed with 5～15% solution, is heated to 50～70 ℃, stir, make its dissolving, be cooled to 35～45 ℃;
2. lactic acid bacteria bacterium mud is joined in the wall material solution, bacterium mud and wall material solution weight volume ratio are between 1: 5～1: 10, rotating speed 1000～4000rpm, even matter emulsification;
3. emulsion spray drying, 160～180 ℃ of inlet temperatures, 70～90 ℃ of outlet temperatures, atomizer rotating speed 20000rpm obtains the microencapsulation lactobacillus preparation.
7, the preparation method of microcapsule compound feed additive according to claim 6 is characterized in that described natural plant gum is Arabic gum or agar; Described protein is any in zein, soybean protein, the skimmed milk power; Described starchy material is modified starch and/or maltodextrin.
8, the preparation method of microcapsule compound feed additive according to claim 5 is characterized in that preparing the microencapsulation eucommia ulmoides extracts and comprises following steps:
1. the preparation of wall material:
A, wall material are formed: natural plant gum and starchy material, the weight proportion of mentioned component are 1: 0.5～2: 1～5;
B, the wall material is mixed with 10～20% solution, is heated to 50～70 ℃, stir, make its dissolving, be cooled to 35～45 ℃;
2. eucommia ulmoides extracts is joined in the wall material solution, the w/v of eucommia ulmoides extracts and wall material solution is 1: 5～1: 10, rotating speed 1000～4000rpm, even matter emulsification;
3. emulsion spray drying, 160～180 ℃ of inlet temperatures, 70～90 ℃ of outlet temperatures, atomizer rotating speed 20000rpm obtains the microencapsulation eucommia ulmoides extracts.
9, the preparation method of microcapsule compound feed additive according to claim 8 is characterized in that described natural plant gum is Arabic gum or agar; Described starchy material is modified starch and/or maltodextrin.
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