CN101586126A - Solid-state fermentation of straw resources ferment 2, the 3-butyleneglycol is produced the method for clean fuel - Google Patents
Solid-state fermentation of straw resources ferment 2, the 3-butyleneglycol is produced the method for clean fuel Download PDFInfo
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- CN101586126A CN101586126A CNA2009100121664A CN200910012166A CN101586126A CN 101586126 A CN101586126 A CN 101586126A CN A2009100121664 A CNA2009100121664 A CN A2009100121664A CN 200910012166 A CN200910012166 A CN 200910012166A CN 101586126 A CN101586126 A CN 101586126A
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- butyleneglycol
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- state fermentation
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- stalk
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- 238000000034 method Methods 0.000 title claims abstract description 31
- 238000010563 solid-state fermentation Methods 0.000 title claims abstract description 24
- 239000000446 fuel Substances 0.000 title claims abstract description 22
- 239000010902 straw Substances 0.000 title claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 239000001913 cellulose Substances 0.000 claims abstract description 19
- 229920002678 cellulose Polymers 0.000 claims abstract description 19
- 239000002994 raw material Substances 0.000 claims abstract description 18
- 239000000463 material Substances 0.000 claims abstract description 14
- 238000004821 distillation Methods 0.000 claims abstract description 13
- 108090000790 Enzymes Proteins 0.000 claims abstract description 8
- 102000004190 Enzymes Human genes 0.000 claims abstract description 8
- 238000009835 boiling Methods 0.000 claims abstract description 3
- 238000002360 preparation method Methods 0.000 claims abstract description 3
- 241000894006 Bacteria Species 0.000 claims description 25
- 239000002054 inoculum Substances 0.000 claims description 16
- 238000000855 fermentation Methods 0.000 claims description 12
- 230000004151 fermentation Effects 0.000 claims description 12
- 241000588748 Klebsiella Species 0.000 claims description 11
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 10
- 239000002253 acid Substances 0.000 claims description 10
- 239000008103 glucose Substances 0.000 claims description 10
- 239000001888 Peptone Substances 0.000 claims description 9
- 108010080698 Peptones Proteins 0.000 claims description 9
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 9
- 235000019319 peptone Nutrition 0.000 claims description 9
- 238000004321 preservation Methods 0.000 claims description 6
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 claims 2
- 229940058015 1,3-butylene glycol Drugs 0.000 claims 1
- 235000019437 butane-1,3-diol Nutrition 0.000 claims 1
- OWBTYPJTUOEWEK-UHFFFAOYSA-N butane-2,3-diol Chemical compound CC(O)C(C)O OWBTYPJTUOEWEK-UHFFFAOYSA-N 0.000 abstract description 15
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 239000002699 waste material Substances 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 3
- 238000000926 separation method Methods 0.000 abstract description 3
- 244000025254 Cannabis sativa Species 0.000 abstract description 2
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- 238000004064 recycling Methods 0.000 abstract description 2
- 108010059892 Cellulase Proteins 0.000 description 7
- 229940106157 cellulase Drugs 0.000 description 7
- 229940088598 enzyme Drugs 0.000 description 5
- 241000209140 Triticum Species 0.000 description 4
- 235000021307 Triticum Nutrition 0.000 description 4
- 240000008042 Zea mays Species 0.000 description 4
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
- 235000009973 maize Nutrition 0.000 description 4
- 229920002488 Hemicellulose Polymers 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 238000009413 insulation Methods 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- 238000010411 cooking Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 230000003113 alkalizing effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- ACGUYXCXAPNIKK-UHFFFAOYSA-N hexachlorophene Chemical compound OC1=C(Cl)C=C(Cl)C(Cl)=C1CC1=C(O)C(Cl)=CC(Cl)=C1Cl ACGUYXCXAPNIKK-UHFFFAOYSA-N 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 239000010808 liquid waste Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000010815 organic waste Substances 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Solid-state fermentation of straw resources ferment 2, the 3-butyleneglycol is produced the method for clean fuel, cellulosic material in the stalk that obtains with the cellulosic material in the stalk that alkalization obtains, liquefaction process in paper industry or the cellulosic material in the stalk that the alkalization boiling obtains, wherein Mierocrystalline cellulose 60～71%, pulverize standby; Get above-mentioned raw materials and add cellulose complex enzyme preparation (Cellulose Multi-enzyme) according to 2～5% enzyme concentration, water addition ratio is pressed 60～75% of raw material weight, and enzymolysis is 3～5 days under the condition of pH5.5～7.0,35～45 ℃; After solid state fermentation, distillation, obtain 2, the 3-butyleneglycol.The present invention uses grass to replace provisions, turns waste into wealth, and has reduced by 2,3-butyleneglycol production cost; Solid-liquid separation is easy in the waste liquid, and is also recycling, therefore reduced environmental pollution.Have the low characteristics of production cost, play the double effects of the protection environment of exploiting natural resources.
Description
Technical field
The invention belongs to the biomass energy field, relate in particular to a kind of biological enzyme and produce 2 in stalk (rice straw, maize straw, wheat stalk) resource, the utilisation technology in the 3-butyleneglycol clean fuel.
Technical background
2,3-butyleneglycol fuel can be inexhausted as renewable energy source, and can not cause Greenhouse effect.Microbial fermentation sugar can produce 2,3-butyleneglycol fuel.In industrial production, be used for fermentation at present and produce 2, if the microbial host bacterium-klebsiella (Klebsiella) of 3-butyleneglycol or Bacillussubtilis genus (Bacillus) etc.The biomass that contain glucide that comprise stalk all may be as 2, the raw material of 3-butyleneglycol fermentation, and the utilization of macromolecular substance needs the degraded of process enzyme earlier.Biology 2,3-butyleneglycol are as the surrogate of oil, and its industrial chain is also continuing extension.There is development Mierocrystalline cellulose system 2 in China, the favourable condition of 3-butyleneglycol, every year, only rice straw just had more than 300,000,000 ton (dry weight), and China's grain resource is not abundant, therefore agriculture and forestry organic waste material is converted into 2,3-butyleneglycol fuel, the formation industrialization utilizes, be fit to very much the national conditions of China, also be very favourable from the energy security angle, and can eliminate the environmental problem that causes by crop straw burning.
Existing stalk (rice straw, maize straw, wheat stalk) resource produces 2, and no matter the cleaning technique of 3-butyleneglycol fuel as being raw material with full stalk, adopt acid hydrolyzation or enzymolysis process to produce 2,3-butyleneglycol clean fuel; Wherein a large amount of hemicelluloses, xylogen and ash content remain in the fermented liquid, bring the difficult problem of follow-up liquid waste disposal to be difficult to so far solve.
Summary of the invention
The objective of the invention is with paper industry after alkalizing or the Mierocrystalline cellulose that obtains of liquefaction process be main raw material, handle through biotechnology, produce biological cheaply 2,3-butyleneglycol fuel.Though and the present invention is characterized in that it selects stalk for use is raw material, but wherein a large amount of hemicellulose, xylogen and ash separation gone out by alkaline process or liquefaction process, and the Mierocrystalline cellulose (hexosan) that obtains is converted into glucose, insert bacterium-Klebsiella (Klebsiella) or Bacillussubtilis and belong to (Bacillus), the fermentation of saccharification limit, limit carries out 2, the 3-butyleneglycol is produced, and fermentation ends extracts 2 through underpressure distillation, 3-butyleneglycol and obtain novel clean fuel.Use grass to replace provisions, turn waste into wealth, reduced by 2,3-butyleneglycol production cost; Have only a spot of residual hemicellulose, lignin and ash content in the waste liquid, even the raw water hydrolysis and fermentation is incomplete, solid-liquid separation is easy in the waste liquid, and is also recycling, therefore reduced environmental pollution.This 2, the production of 3-butyleneglycol fuel has the low characteristics of production cost, playing exploit natural resources the protection environment double effects.The present invention adopts solid state fermentation, thereby has reached the purpose of water saving.
The objective of the invention is to realize by following technical proposal:
Solid-state fermentation of straw resources ferment 2, the 3-butyleneglycol is produced the method for clean fuel, it is characterized in that may further comprise the steps:
1) cellulosic material or the cellulosic material in the stalk that the alkalization boiling obtains in the raw material stalk preparing to obtain with the cellulosic material in the stalk that alkalization obtains, liquefaction process in paper industry, wherein Mierocrystalline cellulose 60～71%, pulverize standby;
2) enzymolysis is got above-mentioned raw materials and is added the cellulose complex enzyme preparation according to 2～5% enzyme concentration, (CelluloseMulti-enzyme), presses 60～75% of raw material weight at water addition ratio, and enzymolysis is 3～5 days under the condition of pH5.5～7.0,35～45 ℃;
3) solid state fermentation;
4) the distillation underpressure distillation obtains 2, the 3-butyleneglycol.
Described in paper industry the Mierocrystalline cellulose of the cellulosic material in the stalk that alkalization obtains be 60～71%.
Described cellulosic material in the stalk that liquefaction process obtains is 60～68%.
The inoculum size adding acid-producing Klebsiella bacterium that solid state fermentation is preferably by 1～5% belongs to bacterium bacterium liquid, bacterium liquid cultural method; Extractum carnis 1%, peptone 1%, glucose 1%, sodium-chlor 0.5% added water to 100ml (W/V), 30～40 ℃ of heat-preservation fermentations 3～5 days.
Embodiment
Embodiment 1
1 kilogram of the straw cellulose that paper industry obtains after alkalization.Ratio in 2% adds cellulase, and pH value 6.5, water addition ratio are pressed 65% of raw material weight, press 2% inoculum size adding bacterium bacterium liquid then, at 36 ℃ of enzymolysis, insulation solid state fermentation 3 days, solid state fermentation belongs to bacterium bacterium liquid, bacterium liquid cultural method for adding acid-producing Klebsiella bacterium by 2% inoculum size; Extractum carnis 1%, peptone 1%, glucose 1%, sodium-chlor 0.5% adds water to 100ml (W/V), obtains 2,3-butyleneglycol fuel 0.24kg through distillation.
Embodiment 2
1 kilogram of the straw cellulose that liquefaction process obtains.Ratio in 3% adds cellulase, at water addition ratio 70%, and 6.5,40 ℃ of enzymolysis of pH value 3 days.Add bacterium bacterium liquid by 2% inoculum size then, 36 ℃ of heat-preservation fermentations 3 days, solid state fermentation belongs to bacterium bacterium liquid, bacterium liquid cultural method for adding acid-producing Klebsiella bacterium by 2% inoculum size; Extractum carnis 1%, peptone 1%, glucose 1%, sodium-chlor 0.5% adds water to 100ml (W/V), obtains 2,3-butyleneglycol fuel 0.27kg through underpressure distillation.
Embodiment 3
1 kilogram of the Wheat Straw Mierocrystalline cellulose that obtains through alkaline cooking.Ratio in 3% adds cellulase, at pH value 6.0, water addition ratio is pressed 68% of raw material weight, press 3% inoculum size adding bacterium bacterium liquid then, at 38 ℃ of enzymolysis, 36 ℃ of insulation solid state fermentations 3 days, solid state fermentation belongs to bacterium bacterium liquid, bacterium liquid cultural method for the inoculum size adding acid-producing Klebsiella bacterium by 3%; Extractum carnis 1%, peptone 1%, glucose 1%, sodium-chlor 0.5% adds water to 100ml (W/V), obtains 2,3-butyleneglycol fuel 0.26kg through underpressure distillation.
Embodiment 4
1 kilogram of the Wheat Straw Mierocrystalline cellulose that liquefaction process obtains.At water addition ratio 65%, in the ratio adding cellulase of raw material weight 4%, pH value 6.5, press 3% inoculum size adding bacterium bacterium liquid then, 38 ℃ of heat-preservation fermentations 3 days, solid state fermentation belongs to bacterium bacterium liquid, bacterium liquid cultural method for adding acid-producing Klebsiella bacterium by 3% inoculum size; Extractum carnis 1%, peptone 1%, glucose 1%, sodium-chlor 0.5% adds water to 100ml (W/V), obtains 2,3-butyleneglycol fuel 0.28kg through underpressure distillation.
Embodiment 5
1 kilogram of the maize straw Mierocrystalline cellulose that obtains through alkaline cooking.At 68% of water addition ratio raw material weight, the ratio in 3% adds cellulase, pH value 6.0, press 3% inoculum size adding bacterium bacterium liquid then, 36 ℃ of heat-preservation fermentations 3 days, solid state fermentation belongs to bacterium bacterium liquid, bacterium liquid cultural method for adding acid-producing Klebsiella bacterium by 3% inoculum size; Extractum carnis 1%, peptone 1%, glucose 1%, sodium-chlor 0.5% adds water to 100ml (W/V), obtains 2,3-butyleneglycol 0.22kg through underpressure distillation.
Embodiment 6
1 kilogram of the maize straw Mierocrystalline cellulose that liquefaction process obtains.Ratio in 4% adds cellulase, presses 70% of raw material weight at water addition ratio, pH value 6.0, press 2% inoculum size adding bacterium bacterium liquid then, 40 ℃ of heat-preservation fermentations 3 days, solid state fermentation belongs to bacterium bacterium liquid, bacterium liquid cultural method for adding acid-producing Klebsiella bacterium by 2% inoculum size; Extractum carnis 1%, peptone 1%, glucose 1%, sodium-chlor 0.5% adds water to 100ml (W/V), obtains 2,3-butyleneglycol 0.26kg through underpressure distillation.
Embodiment 7
1 kilogram of the straw cellulose that paper industry obtains after alkalization.Ratio in 5% adds cellulase, and pH value 7, water addition ratio are pressed 60% of raw material weight, press 5% inoculum size adding bacterium bacterium liquid then, at 36 ℃ of enzymolysis, insulation solid state fermentation 3 days, solid state fermentation belongs to bacterium bacterium liquid, bacterium liquid cultural method for adding acid-producing Klebsiella bacterium by 5% inoculum size; Extractum carnis 1%, peptone 1%, glucose 1%, sodium-chlor 0.5% adds water to 100ml (W/V), obtains 2,3-butyleneglycol fuel 0.26kg through distillation.
Claims (4)
1, the solid-state fermentation of straw resources ferment 2, and the 3-butyleneglycol is produced the method for clean fuel, it is characterized in that may further comprise the steps:
1) cellulosic material or the cellulosic material in the stalk that the alkalization boiling obtains in the raw material stalk preparing to obtain with the cellulosic material in the stalk that alkalization obtains, liquefaction process in paper industry, wherein Mierocrystalline cellulose 60～71%, pulverize standby;
2) enzymolysis is got above-mentioned raw materials and is added cellulose complex enzyme preparation (CelluloseMulti-enzyme) according to 2～5% enzyme concentration, presses 60～75% of raw material weight at water addition ratio, and enzymolysis is 3～5 days under the condition of pH5.5～7.0,35～45 ℃;
3) solid state fermentation;
4) the distillation underpressure distillation obtains 2, the 3-butyleneglycol.
2, solid-state fermentation of straw resources ferment 2 as claimed in claim 1,3-butyleneglycol are produced the method for clean fuel, it is characterized in that described in paper industry the Mierocrystalline cellulose of the cellulosic material in the stalk that alkalization obtains be 60～71%.
3, solid-state fermentation of straw resources ferment 2 as claimed in claim 1, the 3-butyleneglycol is produced the method for clean fuel, it is characterized in that described cellulosic material in the stalk that liquefaction process obtains is 60～68%.
4, as any described solid-state fermentation of straw resources ferment 2 in the claim 1～3, the 3-butyleneglycol is produced the method for clean fuel, it is characterized in that the inoculum size adding acid-producing Klebsiella bacterium that solid state fermentation is specially by 1～5% belongs to bacterium bacterium liquid, bacterium liquid cultural method; Extractum carnis 1%, peptone 1%, glucose 1%, sodium-chlor 0.5% added water to 100ml (W/V), 30-40 ℃ of heat-preservation fermentation 3～5 days.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100121664A CN101586126A (en) | 2009-06-22 | 2009-06-22 | Solid-state fermentation of straw resources ferment 2, the 3-butyleneglycol is produced the method for clean fuel |
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2009100121664A CN101586126A (en) | 2009-06-22 | 2009-06-22 | Solid-state fermentation of straw resources ferment 2, the 3-butyleneglycol is produced the method for clean fuel |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102776244A (en) * | 2012-06-21 | 2012-11-14 | 北京化工大学 | Process for producing polyatomic sugar alcohol and lignin by comprehensively using agricultural and forestry wasters of corncobs |
| WO2013054874A1 (en) * | 2011-10-14 | 2013-04-18 | 東レ株式会社 | Process for producing 2,3-butanediol |
-
2009
- 2009-06-22 CN CNA2009100121664A patent/CN101586126A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013054874A1 (en) * | 2011-10-14 | 2013-04-18 | 東レ株式会社 | Process for producing 2,3-butanediol |
| JPWO2013054874A1 (en) * | 2011-10-14 | 2015-03-30 | 東レ株式会社 | Method for producing 2,3-butanediol |
| EP2767589A4 (en) * | 2011-10-14 | 2015-07-01 | Toray Industries | Process for producing 2,3-butanediol |
| US10584084B2 (en) | 2011-10-14 | 2020-03-10 | Toray Industries, Inc. | Process for producing 2,3-butanediol |
| CN102776244A (en) * | 2012-06-21 | 2012-11-14 | 北京化工大学 | Process for producing polyatomic sugar alcohol and lignin by comprehensively using agricultural and forestry wasters of corncobs |
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Application publication date: 20091125 |