CN101581724A - Special multichannel syphilis diagnosis device combined with quasi-one-dimensional specific antigen modified electrodes - Google Patents
Special multichannel syphilis diagnosis device combined with quasi-one-dimensional specific antigen modified electrodes Download PDFInfo
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Abstract
The invention relates to a special multichannel syphilis diagnosis device combined with quasi-one-dimensional specific antigen modified electrodes. The device is a micro-fluidic chip, belonging to the field of analysis test. One of the targets of the relevant diagnosis and treatment technologies is to quickly diagnose syphilis patients with low cost. The invention provides a diagnosis device aiming to achieve the target. The key points of the proposal are that the device, namely the micro-fluidic chip, comprises channels of parallel structure inside, the parallel structure comprises four branch channels mutually connected in parallel, four catenulate working electrodes in total are respectively arranged in the four branch channels, the catenulate working electrode is composed of a conductive electrode and a gold size sensing membrane that attaches onto the conductive electrode and is embedded with the syphilis specific antibody, and the respective gold size sensing membranes on the superficial coats of the four catenulate working electrodes are respectively embedded with four different syphilis specific antibody substances which are respectively syphilis specific antibodies TP0684, TP0453, TP0821 and TP0319.
Description
Technical field
The present invention relates to a kind of special multichannel syphilis diagnosis device of combined with quasi-one-dimensional specific antigen modified electrodes, this controller used in syphilis diagnosis device is based on the micro-fluidic chip special that the antigen/antibody specific reaction is diagnosed syphilis antigen, belongs to the analytical test field.
Background technology
Syphilis (Syphilis) be by microspironema pallidum (T.pallidum, a kind of sexually transmitted disease that TP) causes (Sexually transmitted disease, STD).Microspironema pallidum only infects the mankind under natural situation, and the people is the unique infection sources of syphilis, main trafficability characteristic contact transmission.Also can pass through the placenta vertical transmission in addition, cause fetal congenital syphilis.A few peoples also can infect through blood transfusion.Since the eighties, syphilis is revivable once more in China, according to national venereal disease control center statistics, China's syphilis incidence of disease annual growth reaches 52.7%, and the congenital syphilis and the neurolues incidence of disease increase obviously, the trend that rises is gradually also arranged in the whole world, syphilis not only become international serious society and public health problem, and be one of principal disease of harm population health.Therefore, select simple, fast, hypersensitivity and specificity diagnostic method and reagent, to the syphilis early diagnosis, find the infection sources, control syphilis is propagated significant.
Controller used in syphilis diagnosis method commonly used at present has etiological examination, non-syphilitic leptospira antigen serum test, TP quick detection test paper bar, microspironema pallidum hemagglutination test (TPHA) and polymerase chain reaction (PCR) etc.The principle of etiological examination is to get the exudate of patient's infringement or the tissue fluid that lymphnode puncture obtains, observe spirochetal characteristic morphologic and mode of motion, in theory early syphilis there is higher recall rate, but the restriction (comprising prescription on individual diagnosis medication, high-quality fluorescent microscope, reagent and technology etc.) that is subjected to condition is also more, and therefore actual recall rate is not high.Though TP quick detection test paper bar has overcome defectives such as length detection time, reagent costliness, domestic temporarily do not have its sensitivity of play-by-play and specificity effect, so be not useable for confirmed diagnosis test.The principle of non-syphilitic leptospira antigen serum test is the serology primary dcreening operation test commonly used of current diagnosis syphilis, though in a lot of documents, all mention non-syphilitic leptospira antigen serum test simple and easy, quick, be easy to observations, the screening serum that is suitable for a large amount of crowds, but susceptibility, specificity are relatively poor, biologic fale-positive and false negative reaction appear sometimes, cause omission and flase drop easily, so be not suitable for the diagnosis of primary syphilis, congenital syphilis, neurolues.Though its susceptibility of TPHA and specificity height mainly are used to the syphilis confirmed diagnosis test, and observation of curative effect is had little significance.The PCR method is newer diagnostic method, and it can carry out early stage antidiastole to genital ulcer, distinguishes syphilis, genital herpes and chancroid.But the PCR of current diagnosis syphilis still exist primer non-specific, to shortcomings such as susceptibility such as serum/whole blood are low.And when the skin damage begins healing, secretion minimizing, serological test positive reaction, adopt PCR method just improper.In addition, PCR method is subject to materials such as tissue in the sample and cell fragment and suppresses, and causes the PCR false negative result.So PCR detects should not be as declaring more.
Detection methods such as TP quick detection test paper bar, non-syphilitic leptospira antigen serum test, TPHA and PCR all belong to immunoassay.Above immunoassay itself has very strong selectivity, to the early diagnosis of infectious disease, judges that all there is important meaning aspects such as curative effect and prognosis.Yet the detection of serious infectious diseases is very complicated, and traditional immunoassay only detects at single antigen usually.Because a kind of diagnostic antigen can appear at different pathogenic bacteria, or different diagnostic antigens can occur simultaneously in same pathogenic bacteria, therefore, carry out multiple efficiently immune analysis method (SMIAs), the kind and the quantity of, quantitative response various pathogenic bacteria antigens qualitative truly from the angle of biological correlativity, in early days prevention and the multiple infectious disease of joint inspection, judge that aspects such as disease carrying germ development degree, O﹠A result of treatment all have important medical value.
Electrochemical immunosensor has unique advantages such as automaticity height, cheapness, easily preparation, rapid sensitive, cooperates immunoreactive efficiently single-mindedly, is fit to very much the analyser of the multiple immunoassay detection of structure serious infectious diseases.The micro-fluidic chip (lab-on-chip) that the title of " life science integrated circuit " is arranged is the desirable technique that makes up microminiaturized, integrated electrochemical immunosensor.The micro-fluidic chip technology be with sampling, pre-service, add reagent, reaction, separation, detection etc. be integrated in finish on the microchip one in front of the door along technology, have analysis speed fast, contain much information, reagent consumption less, advantage such as pollution is little, operation cost is low, instrument is easy to use.And be fit to very much suitability for industrialized production.On behalf of the 21 century analytical instrument, microflow control technique move towards microminiaturized, integrated developing direction.The micro-fluidic Amperometric Detection Coupled chip that the serious infectious diseases that are suitable for structure detect has following advantage: (1) is easy to integrated a plurality of sense channels and detecting electrode on chip by micro-processing technology, accomplishes that multiple target objects analyzes the technical feasibility height simultaneously.(2) by the micro-flowing injection technology, sample flows in pipeline and has guaranteed that electrode surface upgrades constantly, and it is easily contaminated better to have overcome electrode, causes false positive rate height, problem such as collimation difference as a result.(3) microfluidic sensing device upper pipeline diameter has only μ m level, and the reaction tank volume also has only μ L level, and required reagent dosage is few during analysis, and analyte arrives the diffusion length of electrode surface lacks, therefore can significantly reduce the incubation time, realizes the fast joint inspection target.(4) the microfluidic sensing device is many based on the optical detection system at present, and the expensive volume of instrument is big, fully integrated being difficult to, and also it is very high to detect cost, has limited its popularization.And adopting Electrochemical Detection in the sensor, volume is little, automaticity is high, cost is low.
At present, in the micro-fluidic chip technical field, utilize that the multiple specific antibody of syphilis detects simultaneously, the correlation technique and the method for the multiple syphilis antigen of quick diagnosis are not appeared in the newspapers as yet.
Summary of the invention
Technical matters to be solved by this invention is, in the so total technological frame of micro-fluidic chip technology, develop a kind of can utilize the multiple specific antibody of syphilis come to multiple syphilis antigen detect simultaneously, the micro-fluidic chip special of quick diagnosis.
The present invention solve the technical problem by following scheme, the device that this scheme provides is a kind of special multichannel syphilis diagnosis device of combined with quasi-one-dimensional specific antigen modified electrodes, this device is a micro-fluidic chip, the structure of this micro-fluidic chip comprises that applying is installed in two plate objects together, described two plate objects are respectively the cover plate of micro-fluidic chip and the substrate of micro-fluidic chip, on the position of the mutual applying between described two plate objects, be equiped with pipeline, and, three pond shape things, one end of pipeline via the manifold shape fluid passage respectively with wherein two pond shape thing UNICOMs, the other end of pipeline and a remaining pond shape thing UNICOM, and, be installed in the described pipeline working electrode on the diverse location in regular turn respectively and to electrode and contrast electrode, described working electrode by conductive electrode and be attached to embedding on the described conductive electrode gold size sensitive membrane of syphilis specific antibody constitute, this case special feature is, the structure of described pipeline is structure in parallel, the described pipeline that is structure in parallel is made of four lateral parallel connections, and, the quantity of described working electrode is four, the installation position of these four working electrodes lays respectively in described four laterals, and, specific antibody in its top layer gold size sensitive membrane structure of these four working electrodes is respectively four kinds of syphilis antibody materials to the combination of syphilis antigen energy specificity, these four kinds of antibody materials are respectively syphilis specific antibody TP0684, TP0453, TP0821 and TP0319, and its pattern of each working electrode all is beading.
Described gold size sensitive membrane is that shitosan gold size solution and syphilis specific antibody-solutions are fully mixed, and its drying and forming-film is formed.Syphilis specific antibody in the described gold size sensitive membrane is the syphilis antibody of horseradish peroxidase or glucose carbohydrate oxidase mark, described gold size sensitive membrane has been included as fixing above-mentioned each syphilis specific antibody and has introduced wherein complementary medium, and described complementary medium is wherein a kind of of shitosan, cellulose acetate, gelatin or their potpourri for example.
Described pipeline in the described microfluidic chip structure and described lateral and described manifold shape fluid passage, its internal diameter size all can be selected arbitrarily size, but, for as far as possible less with the consideration of aspects such as liquid sample to be measured and reduction reagent loss, described pipeline and described lateral and described manifold shape fluid passage are preferably all selected the passage of capillary level for use, the passage meaning of described capillary level is a capillary channel, and the internal diameter capillaceous on its internal diameter and the ordinary meaning is suitable.The shape of cross section of its inner passage of described kapillary can be a shape arbitrarily, described shape of cross section is for example circular, oval, square, rectangle, bar shaped, can certainly be have bending arbitrarily linear, and, described interior shape capillaceous is along with the extension of pipeline, and it is different shapes that the shape of cross section of different parts also can allow.Only with regard to kapillary one speech, its art-recognized meanings is known.
What relate in the structure is minute sized electrode to electrode and contrast electrode, and its electrode shape all can be selected arbitrarily shape, and described selected arbitrarily shape is square shape sheet shape, rectangle sheet, strip or round sheet or the like for example.
Working electrode in this case chip is beading, its beading electrode profile helps to expand significantly its effective working interface, and, further, the beading pattern also makes this working electrode have good flow-disturbing, and this helps the forced working electrode and the contacting between its peripheral measured object liquid stream of flowing through.
Relate to several pond shape things in this case microfluidic chip structure, described pond shape thing is pond shape or the scrotiform structure that is used for transitional liquid storage, its shape of inner chamber of each pond shape thing wherein all can be selected arbitrarily shape, and described cavity shape is cylindrical cavity shape, square column type cavity-like, oblong cavity shape or spherical hollow space shape or the like for example.The size of described pond shape thing can be arbitrarily selected size, still, for can be as far as possible less with liquid sample to be measured and reduce reagent loss, the hypomegetic pond shape thing that described pond shape thing preferably can mate with kapillary.
This case device can further include some annexes certainly, and described annex is multiple tracks electrochemical workstation and microfluidic pump or the like for example, and the art-recognized meanings of the art-recognized meanings of described multiple tracks electrochemical workstation and microfluidic pump is known.Each beading working electrode that relates in this case microfluidic chip structure and to electrode and contrast electrode etc., can get lines crossed via corresponding special use is respectively connecting with the corresponding interface of described multiple tracks electrochemical workstation.It is to be used for the private cable that each the corresponding interface with each described electrode and described multiple tracks electrochemical workstation is coupled to each other that described special use is being got lines crossed.Described microfluidic pump is exclusively used in and drives micro liquid and flow, described microfluidic pump can with the described pond of selected any one shape thing UNICOM as required.
Described pattern is catenate working electrode, both can be the beading conductive electrode of integrated compression moulding, also can be the beading conductive electrode of moulding by casting; It is the magnetic bead string structure of electric conductivity that its structure of described working electrode can certainly allow, under this situation, it is the tri-iron tetroxide material with conductive capability that described pattern is its material of catenate working electrode, and it is to add the accurate one-dimensional electric electrode that is spliced mutually under the magnetic field condition guiding by the magnetic bead of many tri-iron tetroxide materials that described pattern is catenate working electrode.
Described pattern is catenate working electrode, it is the working electrode of described magnetic bead string structure, magnetic bead diameter in its structure is not limit, the diameter of described magnetic bead can be the size of setting as required arbitrarily, but, the preferred diameter range of described magnetic bead is between 20 nanometer to 2000 nanometers, and, it is the number of setting as required arbitrarily that the number of the magnetic bead that is stitched together mutually that is contained in the structure of each described working electrode can allow, described number is not limit, but the preferred value of the number of the magnetic bead of a described working electrode of formation is between 100 to 100000.
In view of each described beading working electrode occupation space width less, this working electrode can allow directly to be placed in the capillary channel, narrow relatively fluid passage more helps representing the disturbance ability to peripheral working fluid of beading electrode, and, also help the potentiality of its effective working interface given full play of.
Be installed in the described gold size sensitive membrane of described working electrode surface layer by spraying or the coating of point sample instrument point sample or other appropriate process, it is the thickness of any sample measuring liquid treated generation electrical signals response of setting that its thicknesses of layers can allow, but the in other words conj.or perhaps preferred thickness of the thickness of recommendation is between 10 nanometers and 200 nanometers.
It is any electrical insulating property material that described cover plate and substrate in the chip structure, its material can allow, for example: and polypropylene, glass, or the like, preferred material is a polymethylmethacrylate.
It is the width value that can reach any setting of dependence test purpose that described cover plate in the structure and its width of substrate can allow, and still, preferred described width value is all between 2 centimetres and 4 centimetres; It is the length value of setting arbitrarily that can reach the dependence test purpose that the length value of described cover plate and substrate can allow too, and still, preferred length value or the length value of saying recommendation are between 3 centimetres and 6 centimetres.
It is the thickness of setting arbitrarily of being convenient to the device assembling that its thickness of described cover plate and substrate can allow, the thickness of recommendation or to say preferred thickness be between 0.5 millimeter and 5 millimeters.Less thickness helps saving material.
The using method of this case micro-fluidic chip:
Employing adds Micropump and drives the steady flow in the capillary channel of four-way micro-fluidic chip of liquid stream, utilizes the four-way electrochemical analytical instrument respectively four kinds of controller used in syphilis diagnosis antigens to be detected.
The concrete detection of this case micro-fluidic chip uses step as follows:
1, add blood serum sample liquid in the microtubule road, adding under the Micropump driving, various syphilis antigen molecules are by the syphilis specific antibody capture of the corresponding horseradish peroxidase-labeled of gold size sensitive membrane embedding on the electrode surface in each passage.
2, the syphilis specific antibody of horseradish peroxidase-labeled and the syphilis antigen in the blood serum sample form immune complex.
3, adopt multi-channel electrochemical analyzer, add electron mediators such as catechol, adopt the ampere method to detect the electric current variation that above-mentioned reaction causes, obtain the kind and the content of various analytes thus.
4, the result is carried out analysis-by-synthesis, syphilis antigen is carried out comprehensive diagnos.
Advantage of the present invention is, at a physical device is integrated four beading working electrodes that are coated with four kinds of syphilis specific antibody materials respectively on the micro-fluidic chip, these four beading working electrodes are respectively at four kinds of feature diagnostic antigens that detect syphilis, this case micro-fluidic chip is a kind of controller used in syphilis diagnosis micro-fluidic chip special that can utilize the multiple specific antibody of syphilis to detect simultaneously, and the design feature of its integrated structure and special beading working electrode pattern help to improve syphilis medical diagnosis on disease efficient, reduce the controller used in syphilis diagnosis expense.
Description of drawings
Fig. 1 is this case micro-fluidic chip embodiment organigram, what showed is the form of the perspective under the depression angle of this example structure, do not depict described annex among the figure, and, the detailed pattern of described beading working electrode is not amplified among the figure yet and describe, show.
Among the figure, 1,2,10 is respectively three pond shape things that installation position is different, the 3rd, the manifold shape fluid passage, 4,7,11,14 is respectively different but four laterals of formation parallel with one another UNICOM in parallel structure of installation position, the 5th, the specific antibody material that is installed in its top layer gold size sensitive membrane structure in the lateral 4 is the beading working electrode of syphilis specific antibody TP0684, the 6th, the specific antibody material that is installed in its top layer gold size sensitive membrane structure in the lateral 7 is the beading working electrode of syphilis specific antibody TP0453, the 12nd, the specific antibody material that is installed in its top layer gold size sensitive membrane structure in the lateral 11 is the beading working electrode of syphilis specific antibody TP0821, the 13rd, the specific antibody material that is installed in its top layer gold size sensitive membrane structure in the lateral 14 is the beading working electrode of syphilis specific antibody TP0319, the 8th, to electrode, the 9th, contrast electrode.
Embodiment
In this case embodiment that Fig. 1 showed, this device also is that the structure of micro-fluidic chip comprises that applying is installed in two plate objects together, two plate objects are respectively the cover plate of micro-fluidic chip and the substrate of micro-fluidic chip, on the position of the mutual applying between two plate objects, be equiped with pipeline, and, three pond shape things, these three pond shape things are respectively pond shape things 1, pond shape thing 2 and pond shape thing 10, one end of described pipeline via manifold shape fluid passage 3 respectively with pond shape thing 1 and pond shape thing 2 UNICOMs, the other end of described pipeline and remaining pond shape thing 10 UNICOMs, and, be installed in the described pipeline beading working electrode on the diverse location in regular turn respectively and to electrode 8 and contrast electrode 9, the beading working electrode by conductive electrode and be attached to embedding on the described conductive electrode gold size sensitive membrane of syphilis specific antibody constitute, the structure of described pipeline is structure in parallel, this pipeline that is structure in parallel is made of four lateral parallel connections, these four laterals are respectively lateral 4 and lateral 7 and lateral 11 and lateral 14, and, the quantity of beading working electrode is four, these four beading working electrodes are respectively beading working electrode 5 and beading working electrode 6 and beading working electrode 12 and beading working electrode 13, wherein, beading working electrode 5 is that the specific antibody material in its top layer gold size sensitive membrane structure that is installed in the lateral 4 is the beading working electrode of syphilis specific antibody TP0684, beading working electrode 6 is that the specific antibody material in its top layer gold size sensitive membrane structure that is installed in the lateral 7 is the beading working electrode of syphilis specific antibody TP0453, beading working electrode 12 is that the specific antibody material in its top layer gold size sensitive membrane structure that is installed in the lateral 11 is the beading working electrode of syphilis specific antibody TP0821, and beading working electrode 13 is that the specific antibody material in its top layer gold size sensitive membrane structure that is installed in the lateral 14 is the beading working electrode of syphilis specific antibody TP0319.Do not draw among Fig. 1 as associate members such as the microfluidic pump of annex and multiple tracks electrochemical workstations.Each pond shape thing in this routine structure can be as required with as the microfluidic pump of annex UNICOM by any means.Each beading working electrode in this routine structure and to electrode and contrast electrode can be respectively via special-purpose separately cable or say get lines crossed respectively with as the corresponding cable interface of the multiple tracks electrochemical workstation of annex or say the interface of getting lines crossed and connect.As if be necessary to do a bit extra supplementary notes here: antibody and antigen be relative to each other but different materials, the mutual relationship between them is likened more popularly, like being one to overlap the lock in the lockset and the relation of key.
Claims (8)
1. the special multichannel syphilis diagnosis device of combined with quasi-one-dimensional specific antigen modified electrodes, this device is a micro-fluidic chip, the structure of this micro-fluidic chip comprises that applying is installed in two plate objects together, described two plate objects are respectively the cover plate of micro-fluidic chip and the substrate of micro-fluidic chip, on the position of the mutual applying between described two plate objects, be equiped with pipeline, and, three pond shape things, one end of pipeline via the manifold shape fluid passage respectively with wherein two pond shape thing UNICOMs, the other end of pipeline and a remaining pond shape thing UNICOM, and, be installed in the described pipeline working electrode on the diverse location in regular turn respectively and to electrode and contrast electrode, described working electrode by conductive electrode and be attached to embedding on the described conductive electrode gold size sensitive membrane of syphilis specific antibody constitute, it is characterized in that, the structure of described pipeline is structure in parallel, the described pipeline that is structure in parallel is made of four lateral parallel connections, and, the quantity of described working electrode is four, the installation position of these four working electrodes lays respectively in described four laterals, and, specific antibody in its top layer gold size sensitive membrane structure of these four working electrodes is respectively four kinds of syphilis antibody materials to the combination of syphilis antigen energy specificity, these four kinds of antibody materials are respectively syphilis specific antibody TP0684, TP0453, TP0821 and TP0319, and its pattern of each working electrode all is beading.
2. the special multichannel syphilis diagnosis device of combined with quasi-one-dimensional specific antigen modified electrodes according to claim 1 is characterized in that, described pipeline and described lateral and described manifold shape fluid passage are capillary channel.
3. the special multichannel syphilis diagnosis device of combined with quasi-one-dimensional specific antigen modified electrodes according to claim 1, it is characterized in that, it is the tri-iron tetroxide material with conductive capability that described pattern is its material of catenate working electrode, and it is to add the accurate one-dimensional electric electrode that is spliced mutually under the magnetic field condition guiding by the magnetic bead of many tri-iron tetroxide materials that described pattern is catenate working electrode.
4. the special multichannel syphilis diagnosis device of combined with quasi-one-dimensional specific antigen modified electrodes according to claim 3, it is characterized in that, the diameter of described magnetic bead is between 20 nanometer to 2000 nanometers, and the number of the magnetic bead that is stitched together mutually that is contained in the structure of each described working electrode is between 100 to 100000.
5. the special multichannel syphilis diagnosis device of combined with quasi-one-dimensional specific antigen modified electrodes according to claim 1 is characterized in that, the thickness of described gold size sensitive membrane is between 10 nanometers and 200 nanometers.
6. the special multichannel syphilis diagnosis device of combined with quasi-one-dimensional specific antigen modified electrodes according to claim 1 is characterized in that, the described cover plate in the structure and its material of substrate are polymethylmethacrylate.
7. the special multichannel syphilis diagnosis device of combined with quasi-one-dimensional specific antigen modified electrodes according to claim 1, it is characterized in that, all between 2 centimetres and 4 centimetres, its length is all between 3 centimetres and 6 centimetres for described cover plate in the structure and its width of substrate.
8. the special multichannel syphilis diagnosis device of combined with quasi-one-dimensional specific antigen modified electrodes according to claim 1 is characterized in that, the described cover plate in the structure and its thickness of substrate are all between 0.5 millimeter and 5 millimeters.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102478582A (en) * | 2011-10-08 | 2012-05-30 | 宁波大学 | Multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses simultaneously |
| CN104897654A (en) * | 2015-06-02 | 2015-09-09 | 北京纳迅科技有限公司 | Detection device for micro-fluidic biologic chip and preparation method of detection device |
| CN106290869A (en) * | 2015-05-11 | 2017-01-04 | 宁波大学 | The controller used in syphilis diagnosis micro fluidic device of the Dual Drive CGCM of easily-disassembled framework |
| CN107042125A (en) * | 2016-02-05 | 2017-08-15 | 李榕生 | The controller used in syphilis diagnosis device that sample driving can be removed fast with function element |
| CN107042124A (en) * | 2016-02-05 | 2017-08-15 | 李榕生 | The readily removable controller used in syphilis diagnosis micro fluidic device removed of liquid stream driving building blocks of function |
| CN107121477A (en) * | 2016-02-25 | 2017-09-01 | 葛宇杰 | The controller used in syphilis diagnosis micro flow control chip device of operation principles is coupled based on dual drive |
| CN107138185A (en) * | 2016-03-01 | 2017-09-08 | 李榕生 | The controller used in syphilis diagnosis micro fluidic device of the dual drive CGCM of easily-disassembled framework |
| CN107335482A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | Cheap controller used in syphilis diagnosis micro fluidic device based on rigid interface modification theory |
| CN108059683A (en) * | 2017-12-21 | 2018-05-22 | 江汉大学 | Syphilis restructuring multivalent fusion antigen, syphilis recombinant antigen plasmid and syphilis recombinant antigen bacterial strain and preparation method thereof and kit |
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2009
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102478582A (en) * | 2011-10-08 | 2012-05-30 | 宁波大学 | Multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses simultaneously |
| CN106290869A (en) * | 2015-05-11 | 2017-01-04 | 宁波大学 | The controller used in syphilis diagnosis micro fluidic device of the Dual Drive CGCM of easily-disassembled framework |
| CN104897654A (en) * | 2015-06-02 | 2015-09-09 | 北京纳迅科技有限公司 | Detection device for micro-fluidic biologic chip and preparation method of detection device |
| CN104897654B (en) * | 2015-06-02 | 2018-06-01 | 北京纳迅科技股份有限公司 | A kind of microflow controlled biochip detection device and preparation method |
| CN107042125A (en) * | 2016-02-05 | 2017-08-15 | 李榕生 | The controller used in syphilis diagnosis device that sample driving can be removed fast with function element |
| CN107042124A (en) * | 2016-02-05 | 2017-08-15 | 李榕生 | The readily removable controller used in syphilis diagnosis micro fluidic device removed of liquid stream driving building blocks of function |
| CN107121477A (en) * | 2016-02-25 | 2017-09-01 | 葛宇杰 | The controller used in syphilis diagnosis micro flow control chip device of operation principles is coupled based on dual drive |
| CN107138185A (en) * | 2016-03-01 | 2017-09-08 | 李榕生 | The controller used in syphilis diagnosis micro fluidic device of the dual drive CGCM of easily-disassembled framework |
| CN107335482A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | Cheap controller used in syphilis diagnosis micro fluidic device based on rigid interface modification theory |
| CN108059683A (en) * | 2017-12-21 | 2018-05-22 | 江汉大学 | Syphilis restructuring multivalent fusion antigen, syphilis recombinant antigen plasmid and syphilis recombinant antigen bacterial strain and preparation method thereof and kit |
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Application publication date: 20091118 |