CN101555288A - Extracting refining method of water soluble polysaccharide in berry of nitraria tangutorum bobr - Google Patents
Extracting refining method of water soluble polysaccharide in berry of nitraria tangutorum bobr Download PDFInfo
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- CN101555288A CN101555288A CNA2008100179622A CN200810017962A CN101555288A CN 101555288 A CN101555288 A CN 101555288A CN A2008100179622 A CNA2008100179622 A CN A2008100179622A CN 200810017962 A CN200810017962 A CN 200810017962A CN 101555288 A CN101555288 A CN 101555288A
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- tangutorum bobr
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Abstract
The invention relates to an extracting and refining method of water soluble polysaccharide in berry of nitraria tangutorum bobr, in particular to a method extracting the water soluble polysaccharide from the berry of nitraria tangutorum bobr, which is characterized by comprising the following steps: firstly, refluxing and extracting the berry of nitraria tangutorum bobr by using ethanol with concentration of 70-90 percent at 40-80 DEG C, and filtering the refluxed and extracted berry of nitraria tangutorum bobr to obtain filter residue; secondly, refluxing and extracting the filter residue by using distilled water at 60-90 DEG C, and filtering the refluxed and extracted filter residue to obtain extracting solution; thirdly, concentrating the extracting solution to be 1/10-1/5 of primary volume, adding 3-5 times of ethanol with concentration of 95 percent, settling for 12-24h at 4 DEG C, eccentrically eliminating supernatant fluid and getting precipitation, and cooling and drying the precipitation to obtain nitraria tangutorum bobr polysaccharide powder; fourthly, adding the distilled water to dissolve the nitraria tangutorum bobr polysaccharide powder, removing protein for 8-13 times by using a Sevage method; fifthly, leading the polysaccharide removed protein to be processed by macroporous resin column, eluting by using distilled water until eluent is air color and transparent; sixthly, collecting the eluent, concentrating, dialyzing, depositing in alcohol, getting precipitation, and cooling and drying the precipitation to obtain polysaccharide powder.
Description
Technical field
The present invention relates to a kind of method of from chokeberry (white thorn), extracting water-soluble polysaccharide.Particularly extract the method for Purified Water soluble polysaccharide.
Background technology
White thorn (Nitraria) is zygophyllaceae (Zygophyllceae) Nitraria (Nitraria) shrub, in the Caidamu Basin, Qinghai a large amount of distributions is arranged.Its strong stress resistance, salt tolerant alkali can adapt to harsh abominable habitat, be Desert Area important build the mass-planting thing.Except Ecology Action, also have the medical treatment and the health-care effect of wide spectrum, among the peoplely treat stomach trouble with berry of nitraria tangutorum bobr, maldigestion, symptoms such as Common Cold, headache and dizzy.A large amount of in recent years experiments also prove berry of nitraria tangutorum bobr have reducing blood-fat, hypoglycemic, improve immunizing power, multiple function such as anti-oxidant.Therefore Fructus Nitraria schoberi can be used as the exploitation raw material of green nourishing food, natural health care, medicine.Polysaccharide is the macromolecular substance in the natural organic-compound, is formed by connecting by glycosidic link by monosaccharide groups.Studies have shown that both at home and abroad that more and more polysaccharide is a kind of nonspecific immunity strengthening agent, but enhance immunity power strengthens resistance against diseases, thereby reaches effects such as anti-ageing, antitumor, antiviral germ, anti-hepatitis and anti-AIDS.The natural radioactivity polysaccharide has become the research focus that natural pharmacology and protective foods are learned because of its unique function.In the last few years, the various countries scholar competitively sought and extracted the polysaccharide material with physiologically active from various biomaterials.Extraction method of polysaccharides roughly has water extract-alcohol precipitation at present, supersound extraction, and microwave extraction, membrane separation techniques etc. have been widely used in the extraction of fungus polysaccharide, Aloe polysaccharide, tea polysaccharide, lycium barbarum polysaccharide etc., do not extract the purified report but see relevant white thorn polysaccharide as yet.Therefore, the polysaccharide material among the distinctive chokeberry-Bai Ci in area, research Qaidam has its significance.
The content of invention
The objective of the invention is for a kind of method of extracting refining polysaccharide from berry of nitraria tangutorum bobr is provided.
Technical scheme of the present invention is, the present invention can realize by following measure: a kind of method of extracting refining polysaccharide from berry of nitraria tangutorum bobr, be characterized in may further comprise the steps: 1. with berry of nitraria tangutorum bobr with the ethanolic soln of 70%-90% 40-80 ℃ of following refluxing extraction, filter filter residue; 2. the gained filter residue is with distilled water 60-90 ℃ of refluxing extraction, filter extracting solution; 3. the gained extracting solution is concentrated into the 1/10-1/5 of original volume, adds 95% ethanol of 3-5 times of volume, leave standstill 12-24h under 4 ℃, centrifugal supernatant discarded is got precipitation, lyophilize De Baici Crude polysaccharides powder; 4. the Crude polysaccharides powder adds dissolved in distilled water, Sevage method deproteinated 8-13 time; 5. the proteic polysaccharide soln of Ex-all is through macroporous adsorptive resins, and distilled water is eluted to the effluent liquid water white transparency; 6. collect elutriant and concentrate, dialysis, alcohol precipitation is got precipitation, and lyophilize must be made with extra care polysaccharide powder.
Used raw material is white thorn fresh fruit or exsiccant berry of nitraria tangutorum bobr or the fruit pulp powder of removing seed.
In order to remove oil-soluble impuritieses such as monose in the berry of nitraria tangutorum bobr, oligosaccharide, flavones, partial pigment, described step extraction conditions 1. is: alcohol concn is 70%-90%, and extracting temperature is 40-80 ℃, and solid-liquid ratio is 1: 3-1: 8, extraction time is 1-3 time, each 1-2h.
Described step 2. in used extraction solvent be distilled water, extracting temperature is 60-90 ℃, solid-liquid ratio is 1: 3-1: 10, extraction time is 2-4 time, at every turn 1-3h.
Step detect in 4. protein whether the method for Ex-all be ninhydrin method.
The used macroporous adsorbent resin of the 5. middle decolouring of step is X-5 type or AB-8 type.
The used material of the 6. middle dialysis of step is the dialysis tubing of molecular weight cut-off 3500k.
The present invention has following advantage and effect: 1, the present invention has realized that having carried out twice decolouring in the industrial extraction method 2, this method of dialogue thorn handles (alcohol extracting that water shifts to an earlier date, macroporous adsorptive resins), makes the white thorn polysaccharide pigment impurity content that obtains few.3, technical process of the present invention is simple, and efficient height, solvent for use are ethanol, distilled water, environment is not produced and pollutes.4, product cost is low, and therefore raw material abundance, method practicality flexibly are beneficial to popularization, development and application.
Embodiment
Embodiment one: the white thorn fresh fruit that 1. will pluck with the ethanolic soln of 3 times of volumes 90% 40 ℃ of following refluxing extraction 2 times, each 1.5h, filter filter residue; 2. the gained filter residue is with 70 ℃ of refluxing extraction of 5 times of volume distilled water 3 times, and each 2h merges No. 3 times extracting solution; 3. the gained extracting solution is concentrated into 1/5 of original volume, adds 95% ethanol of 5 times of volumes, leave standstill 12h under 4 ℃, centrifugal supernatant discarded is got precipitation, lyophilize De Baici Crude polysaccharides powder; 4. the Crude polysaccharides powder adds dissolved in distilled water, Sevage method deproteinated 8 times, and triketohydrindene hydrate detects; 5. the proteic polysaccharide soln of Ex-all is through the X-5 macroporous adsorptive resins, and distilled water is eluted to the effluent liquid water white transparency; 6. collect elutriant and concentrate, dialysis, alcohol precipitation is got precipitation, and lyophilize must be made with extra care polysaccharide powder.
Embodiment two: 1. with the exsiccant berry of nitraria tangutorum bobr with the ethanolic soln of 5 times of volumes 80% 60 ℃ of following refluxing extraction 1 time, each 2h, filter filter residue; 2. the gained filter residue is with 60 ℃ of refluxing extraction of 10 times of volume distilled water 2 times, and each 3h merges No. 2 times extracting solution; 3. the gained extracting solution is concentrated into 1/7 of original volume, adds 95% ethanol of 4 times of volumes, leave standstill 24h under 4 ℃, centrifugal supernatant discarded is got precipitation, lyophilize De Baici Crude polysaccharides powder; 4. the Crude polysaccharides powder adds dissolved in distilled water, Sevage method deproteinated 11 times, and triketohydrindene hydrate detects; 5. the proteic polysaccharide soln of Ex-all is through AB-8 type macroporous adsorptive resins, and distilled water is eluted to the effluent liquid water white transparency; 6. collect elutriant and concentrate, dialysis, alcohol precipitation is got precipitation, and lyophilize must be made with extra care polysaccharide powder.
Embodiment three: 1. the exsiccant berry of nitraria tangutorum bobr is removed seed and be used to extract seed oil, remaining fruit pulp powder is as the raw material that extracts polysaccharide.The fruit pulp powder adds 80 ℃ of following refluxing extraction of ethanolic soln 3 times of 8 times of volumes 70%, each 1h, filter filter residue; 2. the gained filter residue is with 90 ℃ of following refluxing extraction of 3 times of volume distilled water 4 times, and each 1h merges No. 4 times extracting solution; 3. the gained extracting solution is concentrated into 1/10 of original volume, adds 95% ethanol of 3 times of volumes, leave standstill 18h under 4 ℃, centrifugal supernatant discarded is got precipitation, lyophilize De Baici Crude polysaccharides powder; 4. the Crude polysaccharides powder adds dissolved in distilled water, Sevage method deproteinated 13 times, and triketohydrindene hydrate detects; 5. the proteic polysaccharide soln of Ex-all is through the X-5 macroporous adsorptive resins, and distilled water is eluted to the effluent liquid water white transparency; 6. collect elutriant and concentrate, dialysis, alcohol precipitation is got precipitation, and lyophilize must be made with extra care polysaccharide powder.
Embodiment four: 1. with the exsiccant berry of nitraria tangutorum bobr with the ethanolic soln of 5 times of volumes 80% 60 ℃ of following refluxing extraction 1 time, each 2h, filter filter residue; 2. the gained filter residue is with 60 ℃ of refluxing extraction of 10 times of volume distilled water 2 times, and each 3h merges No. 2 times extracting solution; 3. the gained extracting solution is concentrated into 1/7 of original volume, adds 95% ethanol of 4 times of volumes, leave standstill 24h under 4 ℃, centrifugal supernatant discarded is got precipitation, lyophilize De Baici Crude polysaccharides powder; 4. the Crude polysaccharides powder adds dissolved in distilled water, Sevage method deproteinated 11 times, and triketohydrindene hydrate detects; 5. the proteic polysaccharide soln of Ex-all is through AB-8 type macroporous adsorptive resins, and distilled water is eluted to the effluent liquid water white transparency; 6. collect elutriant and concentrate, dialysis (the used material of dialysing is the dialysis tubing of molecular weight cut-off 3500k), alcohol precipitation is got precipitation, and lyophilize must be made with extra care polysaccharide powder.
Claims (7)
1, the extraction process for purification of water-soluble polysaccharide in the berry of nitraria tangutorum bobr is characterized in that may further comprise the steps: 1. with berry of nitraria tangutorum bobr with the ethanolic soln of 70%-90% 40-80 ℃ of following refluxing extraction, filter filter residue; 2. the gained filter residue is with distilled water 60-90 ℃ of refluxing extraction, filter extracting solution; 3. the gained extracting solution is concentrated into the 1/10-1/5 of original volume, adds 95% ethanol of 3-5 times of volume, leave standstill 12-24h under 4 ℃, centrifugal abandoning supernatant is got precipitation, lyophilize De Baici Crude polysaccharides powder; 4. the Crude polysaccharides powder adds dissolved in distilled water, Sevage method deproteinated 8-13 time; 5. the proteic polysaccharide soln of Ex-all is through macroporous adsorptive resins, and distilled water is eluted to the effluent liquid water white transparency; 6. collect elutriant and concentrate, dialysis, alcohol precipitation is got precipitation, and lyophilize must be made with extra care polysaccharide powder.
2, the extraction process for purification of water-soluble polysaccharide in the berry of nitraria tangutorum bobr as claimed in claim 1 is characterized in that the fruit pulp powder of used raw material for white thorn fresh fruit or exsiccant berry of nitraria tangutorum bobr or removal seed.
3, the extraction process for purification of water-soluble polysaccharide in the berry of nitraria tangutorum bobr as claimed in claim 1 or 2, it is characterized in that: described step 1. in used alcohol concn be 70%-90%, extracting temperature is 40-80 ℃, solid-liquid ratio is 1: 3-1: 8, extraction time is 1-3 time, each 1-2h.
4, the extraction process for purification of water-soluble polysaccharide in the berry of nitraria tangutorum bobr as claimed in claim 1 or 2, it is characterized in that: described step 2. in used extraction solvent be distilled water, extracting temperature is 60-90 ℃, solid-liquid ratio is 1: 3-1: 10, extraction time is 2-4 time, each 1-3h.
5, as the extraction process for purification of water-soluble polysaccharide in claim 1 or the described berry of nitraria tangutorum bobr, it is characterized in that: described step detect in 4. protein whether the method for Ex-all be ninhydrin method.
6, as the extraction process for purification of water-soluble polysaccharide in claim 1 or the 2 or 5 described berry of nitraria tangutorum bobr, it is characterized in that: the used macroporous adsorbent resin of the 5. middle decolouring of described step is X-5 type or AB-8 type.
7, as the extraction process for purification of water-soluble polysaccharide in claim 1 or the 2 or 5 described berry of nitraria tangutorum bobr, it is characterized in that: the used material of the 6. middle dialysis of described step is the dialysis tubing of molecular weight cut-off 3500k.
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101991641A (en) * | 2010-11-09 | 2011-03-30 | 中国科学院西北高原生物研究所 | Preparation method of antioxidation nitraria tangutorum bobr extractive |
| CN101991156A (en) * | 2010-11-09 | 2011-03-30 | 中国科学院西北高原生物研究所 | Method for processing clarified antioxidative nitraria tangutorum bobr juice |
| CN104530251A (en) * | 2014-12-22 | 2015-04-22 | 中国科学院西北高原生物研究所 | Antioxidant compound polysaccharide and preparation method thereof |
| CN104987428A (en) * | 2015-07-23 | 2015-10-21 | 中国科学院西北高原生物研究所 | Compound enzyme-microwave extraction method for optimizing Nitraria polysaccharide by response surface methodology |
| CN105077215A (en) * | 2015-08-20 | 2015-11-25 | 中国科学院西北高原生物研究所 | Cordyceps sinensis freshness-retaining product taking Nitraria tangutorun polysaccharide as natural bacteriostatic agent |
| CN106519056A (en) * | 2016-11-17 | 2017-03-22 | 青海泰柏特生物科技有限公司 | Method for extracting and separating water-soluble polysaccharide from quinoa bran |
| CN106632718A (en) * | 2016-12-28 | 2017-05-10 | 西安医学院 | Preparation method for rhizoma bletillae polysaccharide |
| CN106721709A (en) * | 2016-12-07 | 2017-05-31 | 青海览进生物科技开发有限公司 | A kind of preparation method of black fruit fructus lycii thickened pulp and black fruit fructus lycii juice |
| CN109251253A (en) * | 2018-08-07 | 2019-01-22 | 浙江大学 | A kind of Rubus chingii polysaccharide and preparation method thereof and preparing the application in liver cell Fatty toxicity damage inhibitors |
| CN110746517A (en) * | 2019-12-06 | 2020-02-04 | 中国科学院新疆理化技术研究所 | Method for optimizing ultrasonic-assisted extraction of Siberian nitraria polysaccharide based on response surface method and application |
| CN111759871A (en) * | 2020-08-22 | 2020-10-13 | 南京益唯森生物科技有限公司 | Method for continuously extracting grease, flavone and polysaccharide from nitraria tangutorum bobr seeds |
| CN111574639B (en) * | 2020-07-08 | 2021-06-08 | 江苏汉邦科技有限公司 | Method for separating and purifying nitraria tangutorum bobr polysaccharide |
| CN113603805A (en) * | 2021-09-03 | 2021-11-05 | 中国科学院西北高原生物研究所 | Preparation method of nitraria tangutorum bobr polysaccharide |
| CN113698505A (en) * | 2021-08-31 | 2021-11-26 | 淮阴工学院 | Nitraria sibirica pall polysaccharide drying method |
| CN116023519B (en) * | 2023-01-16 | 2024-06-07 | 江苏神曲医药有限公司 | Application of nitraria polysaccharide in preparing antitumor drugs and extraction method |
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2008
- 2008-04-08 CN CNA2008100179622A patent/CN101555288A/en active Pending
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101991156A (en) * | 2010-11-09 | 2011-03-30 | 中国科学院西北高原生物研究所 | Method for processing clarified antioxidative nitraria tangutorum bobr juice |
| CN101991156B (en) * | 2010-11-09 | 2012-04-25 | 中国科学院西北高原生物研究所 | Method for processing clarified antioxidative nitraria tangutorum bobr juice |
| CN101991641A (en) * | 2010-11-09 | 2011-03-30 | 中国科学院西北高原生物研究所 | Preparation method of antioxidation nitraria tangutorum bobr extractive |
| CN104530251A (en) * | 2014-12-22 | 2015-04-22 | 中国科学院西北高原生物研究所 | Antioxidant compound polysaccharide and preparation method thereof |
| CN104987428A (en) * | 2015-07-23 | 2015-10-21 | 中国科学院西北高原生物研究所 | Compound enzyme-microwave extraction method for optimizing Nitraria polysaccharide by response surface methodology |
| CN105077215A (en) * | 2015-08-20 | 2015-11-25 | 中国科学院西北高原生物研究所 | Cordyceps sinensis freshness-retaining product taking Nitraria tangutorun polysaccharide as natural bacteriostatic agent |
| CN105077215B (en) * | 2015-08-20 | 2017-05-10 | 中国科学院西北高原生物研究所 | Cordyceps sinensis freshness-retaining product taking Nitraria tangutorun polysaccharide as natural bacteriostatic agent |
| CN106519056A (en) * | 2016-11-17 | 2017-03-22 | 青海泰柏特生物科技有限公司 | Method for extracting and separating water-soluble polysaccharide from quinoa bran |
| CN106721709A (en) * | 2016-12-07 | 2017-05-31 | 青海览进生物科技开发有限公司 | A kind of preparation method of black fruit fructus lycii thickened pulp and black fruit fructus lycii juice |
| CN106632718A (en) * | 2016-12-28 | 2017-05-10 | 西安医学院 | Preparation method for rhizoma bletillae polysaccharide |
| CN106632718B (en) * | 2016-12-28 | 2019-04-19 | 西安医学院 | The preparation method of bletilla polysaccharide |
| CN109251253A (en) * | 2018-08-07 | 2019-01-22 | 浙江大学 | A kind of Rubus chingii polysaccharide and preparation method thereof and preparing the application in liver cell Fatty toxicity damage inhibitors |
| CN109251253B (en) * | 2018-08-07 | 2019-11-19 | 浙江大学 | A kind of Rubus chingii polysaccharide and preparation method thereof and preparing the application in liver cell Fatty toxicity damage inhibitors |
| CN110746517A (en) * | 2019-12-06 | 2020-02-04 | 中国科学院新疆理化技术研究所 | Method for optimizing ultrasonic-assisted extraction of Siberian nitraria polysaccharide based on response surface method and application |
| CN111574639B (en) * | 2020-07-08 | 2021-06-08 | 江苏汉邦科技有限公司 | Method for separating and purifying nitraria tangutorum bobr polysaccharide |
| CN111759871A (en) * | 2020-08-22 | 2020-10-13 | 南京益唯森生物科技有限公司 | Method for continuously extracting grease, flavone and polysaccharide from nitraria tangutorum bobr seeds |
| CN113698505A (en) * | 2021-08-31 | 2021-11-26 | 淮阴工学院 | Nitraria sibirica pall polysaccharide drying method |
| CN113603805A (en) * | 2021-09-03 | 2021-11-05 | 中国科学院西北高原生物研究所 | Preparation method of nitraria tangutorum bobr polysaccharide |
| CN116023519B (en) * | 2023-01-16 | 2024-06-07 | 江苏神曲医药有限公司 | Application of nitraria polysaccharide in preparing antitumor drugs and extraction method |
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