CN101554485A - Air purifying material of microorganism absorption degradation, preparation method and applications thereof - Google Patents
Air purifying material of microorganism absorption degradation, preparation method and applications thereof Download PDFInfo
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Abstract
The invention discloses an air purifying material of microorganism absorption degradation, a preparation method and applications thereof. The air purifying material of microorganism absorption degradation is obtained by embedding biological grains with 2 to 4mm grain diameter of pseudomonades or acinetobacter which are cultured and acclimatized in a crosswise way in calcium alginate gel. The air purifying material of microorganism absorption degradation can be used in the long time purification of formaldehyde and benzene series pollution in rooms with obvious effect. The product has the advantages of high activity of immobilized cells, good stability, effectively reducing the content of chemical pollution of formaldehyde and benzene series pollution in rooms, being beneficial for realizing the recycling, regenerating and reusing of biocatalysts, and seriously avoiding the pollution to room environment by microorganisms. The raw material has low cost, the preparation process is simple, easy and convenient in operation, and product can be applied in different reactors.
Description
Technical field
The invention belongs to air purification field, relate to a kind of material for air purification and preparation method and purposes of microorganism absorption degradation.
Background technology
Along with the progress of science and technology, people are more and more higher to indoor air quality requirement, and chemical pollutant more and more is subjected to people's attention as the pith of indoor air quality.Along with the pursuit of people to interior decoration, a large amount of ornament materials enter into indoor environment, the adhesive in the ornament materials, and Homogenholz and paint etc. all can discharge a large amount of VOCs.Wherein, formaldehyde and benzene series thing are that interior decoration causes the most common and pollutant that harm is the most serious in the air pollution, also are the chemical pollutants that presses for removal most.
Formaldehyde (HCHO) has another name called " formaldehyde ", is a kind of colourless, gas that the intense stimulus abnormal smells from the patient is arranged at normal temperatures, soluble in water, pure and mild ether, and it has the effect of solidifying protein, has active chemical property and biological property.Formaldehyde is a kind of active molecule, and a pair of carbonyl is arranged, meeting and amine (also having the aminoacid in the protein), amino-compound, sulfide and organic purine generation vigorous reaction.It also can form the nucleophilic adduct with microorganism cultures, causes the proteinic interconnection key of DNA-DNA and DNA-, can the sudden change and the mammalian cell of DNA, microorganism be damaged, and carcinogenic.The formaldehyde boiling point is 19.5 ℃, thus when room temperature highly volatile, along with the rising evaporation rate of temperature is accelerated.Being defined as carcinogenic and teratogenesis shape material by World Health Organization (WHO) at present, is the allergen of generally acknowledging, also is one of potential strong mutagen.Benzene series thing (benzene series) is in environmental pollution monitoring and control, typically refer to benzene, toluene, o-Dimethylbenzene, ask 8 kinds of chemical compounds such as dimethylbenzene, xylol, ethylbenzene, styrene, isopropylbenzene, be colourless gas with special aromatic odor, cause poisoning through skin contact and absorption, can cause drowsiness, headache, vomiting etc.The benzene series thing is confirmed as poisonous carcinogen by international cancer research institution.Benzene and benzene compound mainly come from synthetic fibers, plastics, fuel, rubber etc., are hidden in the additive of paint, various coating and various adhesive, the waterproof material, also can be from the burning of fuel and Nicotiana tabacum L..In house decoration, adhesive tape, binding agent, wallpaper, paint and furniture surface all are the sources that benzene and substituted benzene exceed standard.Statistics shows that 12% finishing benzene occurred and exceeded standard, and the toluene exceeding standard rate is 14.4%, and dimethylbenzene has then reached 21.3%.Simultaneously, the pollutant volatilization phases such as formaldehyde, benzene were tens two ten years, and the medium-term and long-term low concentration pollutant that exists in room can not look down upon to the potential threat of health.
Formaldehyde mainly shows aspects such as heterosmia, stimulation, allergy, abnormal pulmonary function, abnormal liver function and immunologic dysfunction to health effects.The formaldehyde-caused cardinal symptom of long-term contact low concentration be shed tears, sneeze, cough, even eye conjunctivitis and bronchitis etc. appear, and, formaldehyde also has genetoxic, in the crowd of occupation contact formaldehyde, the DNA-protein-crosslinking material DPC of peripheral blood leucocyte (DNA-protein Cross-links, DPC) level will increase, and DPC is the molecular biosciences sign of chemical substance toxic action.Meanwhile, benzene just was defined as strong carcinogen by World Health Organization (WHO) in 1993, long-term contact benzene can cause bone marrow and genetic damage, causes leukocyte, thrombocytopenia, causes aplastic anemia, even generation leukemia, in the last few years, a lot of labor hygiene data showed, it is higher that the workman who contacts the benzene series mixture for a long time suffers from the ratio of aplastic anemia, in addition, benzene also can cause the birth defect of fetus.Pollute under the situation that is subjected to generally paying attention in formaldehyde in indoor air, the prevention that indoor benzene pollutes will be an another emphasis problem of improving indoor air quality with administering.
In the Study of Indoor Air Quality field, the method that can effectively remove indoor chemical pollutant is deficient relatively, and is all existing various impassable defectives in varying degrees at present:
Active carbon filtration: though this method can be eliminated some chemical gas and abnormal flavour, active carbon belongs to consumable products, needs often to change.And to some chemical contamination effect very limited (for example formaldehyde).
Plasma: the plasma air purification system utilizes the charged characteristics of oxygen molecule, and electrode such as utilization cathode tube etc. penetrates electronics, and with airborne oxygen molecule ionizing, it can be killed and neutralize and decompose airborne antibacterial and chemical pollutant.Shortcoming is to have oxynitride to pollute, and needs high resh air requirement to be desalinated.Its equipment volume is bigger, and power consumption and price are all higher.
Electrostatic precipitation: cardinal principle is to produce high-pressure electrostatic, in the time of will having air that dust etc. can suck particle by electrode, is attracted and collects.To biological not good enough with the chemical contamination effect.All there is high-voltage electrostatic dust separator in a lot of power plants of China.In recent years, this series products has added ultraviolet pipe and drainage screen both at home and abroad, is developed to the central air-conditioning air cleaning facility, but from volume, installation, price, and limitation is all arranged on the effect.
Photocatalyst: commonly the photocatalyst agent is sprayed on indoor wall, utilizes 400nm light wave in the indoor light or the ultraviolet catalytic in the natural light outside window, purify to produce hydroxyl and anion.Its weakness that purifies intensity and degree of stability is self-evident, so although clean-up effect is arranged, sterilization, the purification standard that reach Ministry of Public Health then are difficult to.
The plant method is handled: many existing achievements in research show that green plants has certain purification to indoor air pollution.Green plants can be by being converted into airborne chemical substance the required nutriment of growth, thereby reduce room air pollution effectively, but this type of purification is very limited, its reason is as follows: what 1, plant carried out is photosynthesis, generally suck the part harmful substance by day, and do not possess this function at night.And people's rule of life generally all is that daytime is outdoor, and night, when the people needed most air cleaning, plant had no idea to play corresponding effect indoor; 2, the absorbable nuisance quality of plant is very limited.The release value of national standard PARA FORMALDEHYDE PRILLS(91,95) requires to be lower than 0.08mg/m
3, the house that general firm finishing is intact, formaldehyde is not less than 0.2mg/m
3, and as absorbing the higher Persian of the formaldehyde efficient Herba pteridii latiusculi that pauses, per hour the formaldehyde of Xi Shouing only is 20 μ g.With an area 30m
2, high 3m, formolite number are 0.2mg/m
3The room be example, need in the room, place the 540 basin Persian Herba pteridii latiusculi that pauses, could after 1 hour, harmful substance be reduced to 0.08mg/m
3, bright and clear in the need assurance room in purification process, and no longer include formaldehyde release.Therefore, plant also only can play auxiliary governance role to harmful substance.
Bioanalysis is removed the essence of volatile organic matter in the air promptly by the metabolic activity in the microorganism growth process, with Organic substance as the required nutrient of growth and the process of decomposition and inversion.The principle of handling waste water with bioanalysis is consistent in this, but because atmosphere and the ideal growing environment of non-microorganism, thus microorganism usually can not be in air ambient normal growth, this makes the application of bioanalysis in air cleaning be restricted.Although bioanalysis is in the application that all has aspect Industrial Wastewater Treatment and the industrial waste gas improvement in various degree at present, also rarely found at home and abroad about the related data and the research of the chemical contamination of microbial method degraded room air, still locate cutting edge technology.
But formaldehyde dehydrogenase and formaldehyde dismutase PARA FORMALDEHYDE PRILLS(91,95) that the present invention utilizes the Rhodopseudomonas bacterial spawn to contain effectively decompose (N.Adroer, " Mechanism of formaldehyde biodegradation by pseudomonas putida "), end product is water and carbon dioxide, can not have a negative impact to indoor environment, safe and reliable.In addition, Rhodopseudomonas and acinetobacter can have Degradation (MarceloHenrique Otenio efficiently equally to indoor benzene series thing as benzene,toluene,xylene etc., " Benzene; toluene and xylene biodegradation by Pseudomonas putida CCMI852 "), at home and abroad in the document to this existing elaborating.
Summary of the invention
The objective of the invention is to overcome deficiency of the prior art, a kind of material for air purification of microorganism absorption degradation is provided.
Second purpose of the present invention provides a kind of preparation method of material for air purification of microorganism absorption degradation.
The 3rd purpose of the present invention provides a kind of purposes of material for air purification of microorganism absorption degradation.
Technical scheme of the present invention is summarized as follows:
A kind of material for air purification of microorganism absorption degradation is that the biologic grain of the particle diameter of Rhodopseudomonas after intersect cultivating domestication or acinetobacter at 2～4mm in the calcium alginate gel embedding.
Described Rhodopseudomonas thalline is pseudomonas pseudoalcaligenes Pseudomonaspseudoalcaligenes (CGMCC1.31), Pseudomonas aeruginosa Pseudomonas.Aeruginosa (CGMCC1.1129), pseudomonas putida Pseudomonasputida (CGMCC1.1130), pseudomonas fluorescens Pseudomonas fluorescens (CICC10271) or pseudomonas testosteroni Pseudomonas testosteroni (CICC20542).
Described acinetobacter thalline is acinetobacter calcoaceticus Acinetobacter calcoaceticus (CGMCC 1.2004).
A kind of preparation method of material for air purification of microorganism absorption degradation comprises the steps:
(1) Rhodopseudomonas or acinetobacter activated with purification cultivate:
(2) preparation A culture medium, B culture medium and C culture medium:
Described A culture medium is full Nutrient medium, and pH is 7.0, consists of:
Described B culture medium is the sugar-free mineral salts medium, and concentration is g/L, consists of:
Solvent is a distilled water;
Described C culture medium is the sugaring mineral salts medium, is that the adding glucose is formulated in the B culture medium, and the concentration of described glucose is 0.37g/L;
(3) Rhodopseudomonas or acinetobacter intersected cultivate domestication:
A culture medium, B culture medium and the C culture medium of step (2) preparation are put into culture dish respectively, make the 15-30mL/ ware;
Be to join the C culture medium of making the B culture medium that contains low concentration formaldehyde in B culture dish and the C culture dish and containing low concentration formaldehyde respectively after 0.01%～0.03% the formalin degerming with mass concentration, every ware adds 1mL;
With mass concentration be join respectively after 0.02%～0.06% the formalin degerming make in B culture dish and the C culture dish contain in concentration formaldehyde the B culture medium and contain in the C culture medium of concentration formaldehyde, every ware adds 1mL;
Be to join the C culture medium of making the B culture medium that contains high-concentration formaldehyde in B culture dish and the C culture dish and containing high-concentration formaldehyde respectively after 0.04～0.12% the formalin degerming with mass concentration, every ware adds 1mL;
With the purification bacterial classification inoculation of described step (1) preparation in the described B culture medium that contains low concentration formaldehyde 25～35 ℃ cultivate 2～3 days after, be transferred in the described C culture medium that contains low concentration formaldehyde 25～35 ℃ and cultivated 2～3 days; Then be transferred in described containing in the B culture medium of concentration formaldehyde 25～35 ℃ and cultivate after 2～3 days, be transferred in the C culture medium of concentration formaldehyde in described containing 25～35 ℃ and cultivated 2～3 days; Being transferred in the described B culture medium that contains high-concentration formaldehyde 25～35 ℃ afterwards cultivated 2～3 days, being transferred in the described C culture medium that contains high-concentration formaldehyde 25～35 ℃ again cultivated 2～3 days, being transferred in the described A culture medium 25～35 ℃ at last cultivated 1～2 day, reach exponential phase or stable phase, get bacterium colony and make solid agar slant preservation strain, cultivate after 1～2 day for 25 ℃～35 ℃, the thalline of having been tamed is preserved stand-by in 4 ℃ of refrigerators;
(4) with calcium alginate embedded thalline of having tamed:
The thalline of having tamed of step (3) preparation is inoculated in the triangular flask that the sterilized nutrient broth fluid medium of 500mL is housed, under 25 ℃～35 ℃ on shaking table constant temperature culture 1～2 day, cell density reaches 10
9-10
10Individual/mL, get bacteria suspension, get the centrifuge tube that the 50mL bacteria suspension injects sterilization, separated 15-30 minute under 4000rpm, separating obtained somatic cells is through sterile saline washing 2～3 times, and adding distil water is to 50mL, somatic cells is suspended in the distilled water, must adds the water bacteria suspension;
Be 100: 0.5～1 ratio by volume with mass concentration be the sodium alginate aqueous solution of 2%-5% and describedly make mixing material after adding water bacteria suspension mixing, with the aciculiform pipe described mixing material is splashed in the CaCl2 aqueous solution that mass concentration is 5%-10%, left standstill 7～8 hours, obtain the granule of 2～5mm, clean with normal saline and distilled water after leaching granule, make a kind of biological absorbed air scavenging material.
Described step (1) is preferably:
Get Rhodopseudomonas or acinetobacter thalline in the lyophilisation pipe, under gnotobasis, inject the nutrient broth fluid medium of 80-120mL, in 1～2 day activated spawn of 25～35 ℃ of following continuous culture of shaking table, utilize the plate streaking partition method to isolate single bacterium colony, continuous culture is 1～2 day in 25～35 ℃ of constant incubators, being transferred to the solid agar slant culture-medium cultivated 1～2 day, in 4 ℃ of refrigerators, preserve stand-by, consisting of of described nutrient broth fluid medium: Carnis Bovis seu Bubali cream 1.5g, peptone 1g, NaCl1.5g, distilled water 100mL; Consisting of of described solid agar slant culture-medium: nutrient broth fluid medium 100mL, agar 4-5g, adjusting pH is 7-7.5.
Described Rhodopseudomonas thalline is pseudomonas pseudoalcaligenes Pseudomonas pseudoalcaligenes (CGMCC1.31), Pseudomonas aeruginosa Pseudomonas.Aeruginosa (CGMCC1.1129), pseudomonas putida Pseudomonasputida (CGMCC1.1130), pseudomonas fluorescens Pseudomonas fluorescens (CICC10271) or pseudomonas testosteroni Pseudomonas testosteroni (CICC20542).
Described acinetobacter thalline is acinetobacter calcoaceticus Acinetobacter calcoaceticus (CGMCC 1.2004).
The application of the material for air purification of microorganism absorption degradation in absorption degradation aerochemistry pollutant.
Advantage of the present invention is:
The material for air purification of microorganism absorption degradation can be used for the long-term purification of indoor formaldehyde and the pollution of benzene series thing, and clean-up effect is remarkable.This product has fixation cell cytoactive height, good stability, can reduce the content of harmful chemical pollutants such as indoor formaldehyde and benzene series thing lastingly effectively, be beneficial to the recovery, regeneration and the reusable advantage that realize biocatalyzer, and the pollution of microorganism to indoor environment avoided in strictness.The prices of raw and semifnished materials are cheap, and processing technology is simple and easy to do, and manufactured goods can be used in multiple reactor, improving field of purification broad market prospect such as indoor air quality and removal chemical pollutant.
Description of drawings
Fig. 1 is the outward appearance stereoscan photograph of the material for air purification of a kind of microorganism absorption degradation of the present invention.
Fig. 2 is the in-house network pore structure stereoscan photograph of the material for air purification of a kind of microorganism absorption degradation of the present invention.
Fig. 3 is the outward appearance of the material for air purification of a kind of microorganism absorption degradation of the present invention.
Fig. 4 is the effect of the material for air purification degraded indoor formaldehyde of microorganism absorption degradation of the present invention.
The specific embodiment
The present invention is further illustrated below in conjunction with specific embodiment.
Embodiment 1
A kind of material for air purification of microorganism absorption degradation is that the biologic grain of the particle diameter of the pseudomonas putida Pseudomonas putida (CGMCC 1.1130) after intersection is cultivated domestication at 2～4mm in the calcium alginate gel embedding.
Utilize calcium alginate gel embedding pseudomonas putida to make the biologic grain of particle diameter at 2～4mm, with microorganism be embedded in aperture that calcium alginate macromolecular chain skeleton forms be about 100～500 μ m than in the big hole, effectively prevented the outside seepage of microbial cell and indoor environment polluted.The calcium alginate gel granule is a kind of three-dimensional ladder-shaper structure system, short texture, be beneficial to material Transfer, the microcellular structure (aperture is about 20-50 μ m) on surface helps micromolecule such as moisture and VOCs and enters, internal structure is loose, be very beneficial for material Transfer, moisture that enters and VOCs molecule are easy to be spread to its system inside by the calcium alginate gel surface, and then the degree of depth that helps the microbial degradation chemical pollutant of gel inside carries out, and therefore can efficiently remove indoor chemical pollutant.
Calcium alginate gel beads is the uneven system of a kind of Density Distribution, ecto-entad can be divided into compacted zone, than compacted zone and loose kernel, the calcium alginate gel surface micropore aperture that is used for the embedding pseudomonas putida among the present invention is about 20-50 μ m, inner mesh aperture is about 100-500 μ m, antibacterial shared volume in calcium alginate gel is about 0.88%, and the biologic grain particle diameter is about 2-4mm.See Fig. 1, Fig. 2 and Fig. 3.
A kind of material for air purification of microorganism absorption degradation is that the biologic grain of the particle diameter of the pseudomonas pseudoalcaligenes Pseudomonas pseudoalcaligenes (CGMCC 1.31) after intersection is cultivated domestication at 2～4mm in the calcium alginate gel embedding.
Embodiment 3
A kind of material for air purification of microorganism absorption degradation is that the biologic grain of the particle diameter of the Pseudomonas aeruginosa Pseudomonas.Aeruginosa (CGMCC1.1129) after intersection is cultivated domestication at 2～4mm in the calcium alginate gel embedding.
A kind of material for air purification of microorganism absorption degradation is that the biologic grain of the particle diameter of the pseudomonas fluorescens Pseudomonas fluorescens (CICC10271) after intersection is cultivated domestication at 2～4mm in the calcium alginate gel embedding.
Embodiment 5
A kind of material for air purification of microorganism absorption degradation is that the biologic grain of the particle diameter of the pseudomonas testosteroni Pseudomonas testosteroni (CICC20542) after intersection is cultivated domestication at 2～4mm in the calcium alginate gel embedding.
A kind of material for air purification of microorganism absorption degradation is that the acinetobacter calcoaceticus Acinetobacter calcoaceticus:(CGMCC 1.2004 after intersect cultivating domestication in the calcium alginate gel embedding) particle diameter at the biologic grain of 2～4mm.
Embodiment 7
A kind of preparation method of material for air purification of microorganism absorption degradation comprises the steps:
(1) pseudomonas putida activated with purification cultivate:
Get pseudomonas putida Pseudomonasputida (CGMCC1.1130) thalline in the lyophilisation pipe, under gnotobasis, inject the nutrient broth fluid medium of 100mL, in 1.5 days activated spawn of 30 ℃ of following continuous culture of shaking table, utilize the plate streaking partition method to isolate single bacterium colony, continuous culture is 1.5 days in 30 ℃ of constant incubators, being transferred to the solid agar slant culture-medium cultivated 1.5 days, in 4 ℃ of refrigerators, preserve stand-by, consisting of of described nutrient broth fluid medium: Carnis Bovis seu Bubali cream 1.5g, peptone 1g, NaCl1.5g, distilled water 100mL; Consisting of of described solid agar slant culture-medium: nutrient broth fluid medium 100mL, agar 5g, regulating pH is 7;
(2) preparation A culture medium, B culture medium and C culture medium:
Described A culture medium is full Nutrient medium, and pH is 7.0, consists of:
Described B culture medium is the sugar-free mineral salts medium, and concentration is g/L, consists of:
Solvent is a distilled water;
Described C culture medium is the sugaring mineral salts medium, is that the adding glucose is formulated in the B culture medium, and the concentration of described glucose is 0.37g/L;
(3) pseudomonas putida intersected cultivate domestication:
A culture medium, B culture medium and the C culture medium of step (2) preparation are put into culture dish respectively, make the 20mL/ ware;
Be to join the C culture medium of making the B culture medium that contains low concentration formaldehyde in B culture dish and the C culture dish and containing low concentration formaldehyde respectively after 0.02% the formalin degerming with mass concentration, every ware adds 1mL;
With mass concentration be join respectively after 0.04% the formalin degerming make in B culture dish and the C culture dish contain in concentration formaldehyde the B culture medium and contain in the C culture medium of concentration formaldehyde, every ware adds 1mL;
Be to join the C culture medium of making the B culture medium that contains high-concentration formaldehyde in B culture dish and the C culture dish and containing high-concentration formaldehyde respectively after 0.08% the formalin degerming with mass concentration, every ware adds 1mL;
With the purification bacterial classification inoculation of described step (1) preparation in the described B culture medium that contains low concentration formaldehyde 30 ℃ cultivated 3 days, being transferred in the described C culture medium that contains low concentration formaldehyde 30 ℃ cultivated 3 days, being transferred in described containing in the B culture medium of concentration formaldehyde 30 ℃ again cultivated 3 days, being transferred in described containing in the C culture medium of concentration formaldehyde 30 ℃ again cultivated 3 days, being transferred in the described B culture medium that contains high-concentration formaldehyde 30 ℃ again cultivated 3 days, being transferred in the described C culture medium that contains high-concentration formaldehyde 30 ℃ again cultivated 3 days, being transferred in the described A culture medium 30 ℃ again cultivated 2 days, reach exponential phase, get bacterium colony and make solid agar slant preservation strain, cultivate after 2 days for 30 ℃, the thalline of having been tamed is preserved stand-by in 4 ℃ of refrigerators;
(4) with calcium alginate embedded thalline of having tamed:
The thalline of having tamed of step (3) preparation is inoculated in the triangular flask that the sterilized nutrient broth fluid medium of 500mL is housed, under 30 ℃ on shaking table constant temperature culture 2 days, cell density reaches 10
10Individual/mL, get bacteria suspension, get the centrifuge tube that the 50mL bacteria suspension injects sterilization, separated 20 minutes under 4000rpm, separating obtained somatic cells is through sterile saline washing 3 times, and adding distil water is to 50mL, somatic cells is suspended in the distilled water, must adds the water bacteria suspension;
The ratio that is 100: 1 by volume is 2% sodium alginate aqueous solution with mass concentration and describedly makes mixing material after adding water bacteria suspension mixing, and with the aciculiform pipe described mixing material being splashed into mass concentration is 8% CaCl
2In the aqueous solution, left standstill 8 hours, obtain the granule of 2～5mm, leach behind the granule and clean, make a kind of biological absorbed air scavenging material with normal saline and distilled water.
A kind of preparation method of material for air purification of microorganism absorption degradation comprises the steps:
(1) pseudomonas pseudoalcaligenes activated with purification cultivate:
Get pseudomonas pseudoalcaligenes Pseudomonas pseudoalcaligenes (CGMCC 1.31) thalline in the lyophilisation pipe, under gnotobasis, inject the nutrient broth fluid medium of 80mL, in 2 days activated spawn of 25 ℃ of following continuous culture of shaking table, utilize the plate streaking partition method to isolate single bacterium colony, continuous culture is 2 days in 25 ℃ of constant incubators, being transferred to the solid agar slant culture-medium cultivated 1 day, preserve stand-byly in 4 ℃ of refrigerators, the composition of nutrient broth fluid medium is identical with embodiment 7; Consisting of of solid agar slant culture-medium: nutrient broth fluid medium 100mL, agar 4g, regulating pH is 7.5;
(2) preparation A culture medium, B culture medium and C culture medium: with embodiment 7;
(3) pseudomonas pseudoalcaligenes intersected cultivate domestication:
A culture medium, B culture medium and the C culture medium of step (2) preparation are put into culture dish respectively, make the 25mL/ ware;
Be to join the C culture medium of making the B culture medium that contains low concentration formaldehyde in B culture dish and the C culture dish and containing low concentration formaldehyde respectively after 0.01% the formalin degerming with mass concentration, every ware adds 1mL;
With mass concentration be join respectively after 0.02% the formalin degerming make in B culture dish and the C culture dish contain in concentration formaldehyde the B culture medium and contain in the C culture medium of concentration formaldehyde, every ware adds 1mL;
Be to join the C culture medium of making the B culture medium that contains high-concentration formaldehyde in B culture dish and the C culture dish and containing high-concentration formaldehyde respectively after 0.04 the formalin degerming with mass concentration, every ware adds 1mL;
With the purification bacterial classification inoculation of described step (1) preparation in the described B culture medium that contains low concentration formaldehyde 35 ℃ cultivated 2 days, being transferred in the described C culture medium that contains low concentration formaldehyde 35 ℃ cultivated 2 days, being transferred in described containing in the B culture medium of concentration formaldehyde 35 ℃ again cultivated 2 days, being transferred in described containing in the C culture medium of concentration formaldehyde 35 ℃ again cultivated 2 days, being transferred in the described B culture medium that contains high-concentration formaldehyde 35 ℃ again cultivated 2 days, being transferred in the described C culture medium that contains high-concentration formaldehyde 35 ℃ again cultivated 2 days, being transferred in the described A culture medium 35 ℃ again cultivated 1 day, reach exponential phase, get bacterium colony and make solid agar slant preservation strain, cultivate after 1 day for 35 ℃, the thalline of having been tamed is preserved stand-by in 4 ℃ of refrigerators;
(4) with calcium alginate embedded thalline of having tamed:
The thalline of having tamed of step (3) preparation is inoculated in the triangular flask that the sterilized nutrient broth fluid medium of 500mL is housed, under 35 ℃ on shaking table constant temperature culture 1 day, cell density reaches 10
9Individual/mL, get bacteria suspension, get the centrifuge tube that the 50mL bacteria suspension injects sterilization, separated 30 minutes under 4000rpm, separating obtained somatic cells is through sterile saline washing 2 times, and adding distil water is to 50mL, somatic cells is suspended in the distilled water, must adds the water bacteria suspension;
The ratio that is 100: 1 by volume is 3% sodium alginate aqueous solution with mass concentration and describedly makes mixing material after adding water bacteria suspension mixing, and with the aciculiform pipe described mixing material being splashed into mass concentration is 7% CaCl
2In the aqueous solution, left standstill 7 hours, obtain the granule of 2～5mm, leach behind the granule and clean, make a kind of biological absorbed air scavenging material with normal saline and distilled water.
Embodiment 9
A kind of preparation method of material for air purification of microorganism absorption degradation comprises the steps:
(1) Pseudomonas aeruginosa activated with purification cultivate:
Get Pseudomonas aeruginosa Pseudomonas.Aeruginosa (CGMCC 1.1129) thalline in the lyophilisation pipe, under gnotobasis, inject the nutrient broth fluid medium of 90mL, in 1 day activated spawn of 35 ℃ of following continuous culture of shaking table, utilize the plate streaking partition method to isolate single bacterium colony, continuous culture is 1 day in 35 ℃ of constant incubators, be transferred to the solid agar slant culture-medium and cultivated 2 days, preserve stand-byly in 4 ℃ of refrigerators, the composition of nutrient broth fluid medium is with embodiment 7; The composition of solid agar slant culture-medium is with embodiment 7;
(2) preparation A culture medium, B culture medium and C culture medium: with embodiment 7;
(3) Pseudomonas aeruginosa intersected cultivate domestication:
A culture medium, B culture medium and the C culture medium of step (2) preparation are put into culture dish respectively, make the 30mL/ ware;
Be to join the C culture medium of making the B culture medium that contains low concentration formaldehyde in B culture dish and the C culture dish and containing low concentration formaldehyde respectively after 0.03% the formalin degerming with mass concentration, every ware adds 1mL;
With mass concentration be join respectively after 0.06% the formalin degerming make in B culture dish and the C culture dish contain in concentration formaldehyde the B culture medium and contain in the C culture medium of concentration formaldehyde, every ware adds 1mL;
Be to join the C culture medium of making the B culture medium that contains high-concentration formaldehyde in B culture dish and the C culture dish and containing high-concentration formaldehyde respectively after 0.12% the formalin degerming with mass concentration, every ware adds 1mL;
With the purification bacterial classification inoculation of described step (1) preparation in the described B culture medium that contains low concentration formaldehyde 25 ℃ cultivated 3 days, being transferred in the described C culture medium that contains low concentration formaldehyde 25 ℃ cultivated 3 days, being transferred in described containing in the B culture medium of concentration formaldehyde 25 ℃ again cultivated 3 days, being transferred in described containing in the C culture medium of concentration formaldehyde 25 ℃ again cultivated 3 days, being transferred in the described B culture medium that contains high-concentration formaldehyde 25 ℃ again cultivated 3 days, being transferred in the described C culture medium that contains high-concentration formaldehyde 25 ℃ again cultivated 3 days, being transferred in the described A culture medium 25 ℃ again cultivated 1 day, reach stable phase, get bacterium colony and make solid agar slant preservation strain, cultivate after 2 days for 25 ℃, the thalline of having been tamed is preserved stand-by in 4 ℃ of refrigerators;
(4) with calcium alginate embedded thalline of having tamed:
The thalline of having tamed of step (3) preparation is inoculated in the triangular flask that the sterilized nutrient broth fluid medium of 500mL is housed, under 25 ℃ on shaking table constant temperature culture 2 days, cell density reaches 10
9Individual/mL, get bacteria suspension, get the centrifuge tube that the 50mL bacteria suspension injects sterilization, separated 15 minutes under 4000rpm, separating obtained somatic cells is through sterile saline washing 3 times, and adding distil water is to 50mL, somatic cells is suspended in the distilled water, must adds the water bacteria suspension;
The ratio that is 100: 0.5 by volume is 2% sodium alginate aqueous solution with mass concentration and describedly makes mixing material after adding water bacteria suspension mixing, and with the aciculiform pipe described mixing material being splashed into mass concentration is 5% CaCl
2In the aqueous solution, left standstill 7 hours, obtain the granule of 2～5mm, leach behind the granule and clean, make a kind of biological absorbed air scavenging material with normal saline and distilled water.
A kind of preparation method of material for air purification of microorganism absorption degradation comprises the steps:
(1) pseudomonas fluorescens activated with purification cultivate:
Get lyophilisation inner fluorescent tube pseudomonas Pseudomonas fluorescens (CICC 10271) thalline, under gnotobasis, inject the nutrient broth fluid medium of 120mL, in 1 day activated spawn of 25 ℃ of following continuous culture of shaking table, utilize the plate streaking partition method to isolate single bacterium colony, continuous culture is 1 day in 25 ℃ of constant incubators, be transferred to the solid agar slant culture-medium and cultivated 2 days, preserve stand-byly in 4 ℃ of refrigerators, the composition of nutrient broth fluid medium is with embodiment 7; The composition of solid agar slant culture-medium is with embodiment 8;
(2) preparation A culture medium, B culture medium and C culture medium: with embodiment 7;
(3) pseudomonas fluorescens intersected cultivate domestication:
A culture medium, B culture medium and the C culture medium of step (2) preparation are put into culture dish respectively, make the 15mL/ ware;
Be to join the C culture medium of making the B culture medium that contains low concentration formaldehyde in B culture dish and the C culture dish and containing low concentration formaldehyde respectively after 0.02% the formalin degerming with mass concentration, every ware adds 1mL;
With mass concentration be join respectively after 0.04% the formalin degerming make in B culture dish and the C culture dish contain in concentration formaldehyde the B culture medium and contain in the C culture medium of concentration formaldehyde, every ware adds 1mL;
Be to join the C culture medium of making the B culture medium that contains high-concentration formaldehyde in B culture dish and the C culture dish and containing high-concentration formaldehyde respectively after 0.08% the formalin degerming with mass concentration, every ware adds 1mL;
With the purification bacterial classification inoculation of described step (1) preparation in the described B culture medium that contains low concentration formaldehyde 28 ℃ cultivated 3 days, being transferred in the described C culture medium that contains low concentration formaldehyde 28 ℃ cultivated 3 days, being transferred in described containing in the B culture medium of concentration formaldehyde 28 ℃ again cultivated 3 days, being transferred in described containing in the C culture medium of concentration formaldehyde 28 ℃ again cultivated 3 days, being transferred in the described B culture medium that contains high-concentration formaldehyde 28 ℃ again cultivated 3 days, being transferred in the described C culture medium that contains high-concentration formaldehyde 28 ℃ again cultivated 3 days, being transferred in the described A culture medium 28 ℃ again cultivated 2 days, reach exponential phase, get bacterium colony and make solid agar slant preservation strain, cultivate after 2 days for 28 ℃, the thalline of having been tamed is preserved stand-by in 4 ℃ of refrigerators;
(4) with calcium alginate embedded thalline of having tamed:
The thalline of having tamed of step (3) preparation is inoculated in the triangular flask that the sterilized nutrient broth fluid medium of 500mL is housed, under 28 ℃ on shaking table constant temperature culture 2 days, cell density reaches 10
10Individual/mL, get bacteria suspension, get the centrifuge tube that the 50mL bacteria suspension injects sterilization, separated 30 minutes under 4000rpm, separating obtained somatic cells is through sterile saline washing 3 times, and adding distil water is to 50mL, somatic cells is suspended in the distilled water, must adds the water bacteria suspension;
The ratio that is 100: 0.8 by volume is 5% sodium alginate aqueous solution with mass concentration and describedly makes mixing material after adding water bacteria suspension mixing, and with the aciculiform pipe described mixing material being splashed into mass concentration is 10% CaCl
2In the aqueous solution, left standstill 8 hours, obtain the granule of 2～5mm, leach behind the granule and clean, make a kind of biological absorbed air scavenging material with normal saline and distilled water.
Embodiment 11
A kind of preparation method of material for air purification of microorganism absorption degradation comprises the steps:
(1) pseudomonas testosteroni activated with purification cultivate:
Get pseudomonas testosteroni Pseudomonas testosteroni (CICC20542) thalline in the lyophilisation pipe, under gnotobasis, inject the nutrient broth fluid medium of 110mL, in 2 days activated spawn of 35 ℃ of following continuous culture of shaking table, utilize the plate streaking partition method to isolate single bacterium colony, continuous culture is 2 days in 35 ℃ of constant incubators, be transferred to the solid agar slant culture-medium and cultivated 1 day, preserve stand-byly in 4 ℃ of refrigerators, the composition of nutrient broth fluid medium is with embodiment 7; The composition of solid agar slant culture-medium is with embodiment 7;
(2) preparation A culture medium, B culture medium and C culture medium: with embodiment 7;
(3) pseudomonas testosteroni intersected cultivate domestication:
A culture medium, B culture medium and the C culture medium of step (2) preparation are put into culture dish respectively, make the 20mL/ ware;
Be to join the C culture medium of making the B culture medium that contains low concentration formaldehyde in B culture dish and the C culture dish and containing low concentration formaldehyde respectively after 0.02% the formalin degerming with mass concentration, every ware adds 1mL;
With mass concentration be join respectively after 0.05 the formalin degerming make in B culture dish and the C culture dish contain in concentration formaldehyde the B culture medium and contain in the C culture medium of concentration formaldehyde, every ware adds 1mL;
Be to join the C culture medium of making the B culture medium that contains high-concentration formaldehyde in B culture dish and the C culture dish and containing high-concentration formaldehyde respectively after 0.10% the formalin degerming with mass concentration, every ware adds 1mL;
With the purification bacterial classification inoculation of described step (1) preparation in the described B culture medium that contains low concentration formaldehyde 28 ℃ cultivated 3 days, being transferred in the described C culture medium that contains low concentration formaldehyde 28 ℃ cultivated 3 days, being transferred in described containing in the B culture medium of concentration formaldehyde 28 ℃ again cultivated 3 days, being transferred in described containing in the C culture medium of concentration formaldehyde 28 ℃ again cultivated 3 days, being transferred in the described B culture medium that contains high-concentration formaldehyde 28 ℃ again cultivated 3 days, being transferred in the described C culture medium that contains high-concentration formaldehyde 28 ℃ again cultivated 3 days, being transferred in the described A culture medium 28 ℃ again cultivated 2 days, reach exponential phase, get bacterium colony and make solid agar slant preservation strain, cultivate after 2 days for 28 ℃, the thalline of having been tamed is preserved stand-by in 4 ℃ of refrigerators;
(4) with calcium alginate embedded thalline of having tamed: with embodiment 7.
A kind of preparation method of material for air purification of microorganism absorption degradation comprises the steps:
(1) acinetobacter calcoaceticus activated with purification cultivate:
Get acinetobacter calcoaceticus Acinetobacter calcoaceticus:(CGMCC 1.2004 in the lyophilisation pipe) thalline, under gnotobasis, inject the nutrient broth fluid medium of 100mL, in 1 day activated spawn of 30 ℃ of following continuous culture of shaking table, utilize the plate streaking partition method to isolate single bacterium colony, continuous culture is 1 day in 30 ℃ of constant incubators, being transferred to the solid agar slant culture-medium cultivated 1 day, preserve stand-byly in 4 ℃ of refrigerators, the composition of nutrient broth fluid medium is with embodiment 7; The composition of solid agar slant culture-medium is with embodiment 8
(2) preparation A culture medium, B culture medium and C culture medium: (with embodiment 7)
(3) acinetobacter calcoaceticus intersected cultivate domestication:
A culture medium, B culture medium and the C culture medium of step (2) preparation are put into culture dish respectively, make the 25mL/ ware;
Be to join the C culture medium of making the B culture medium that contains low concentration formaldehyde in B culture dish and the C culture dish and containing low concentration formaldehyde respectively after 0.03% the formalin degerming with mass concentration, every ware adds 1mL;
With mass concentration be join respectively after 0.06% the formalin degerming make in B culture dish and the C culture dish contain in concentration formaldehyde the B culture medium and contain in the C culture medium of concentration formaldehyde, every ware adds 1mL;
Be to join the C culture medium of making the B culture medium that contains high-concentration formaldehyde in B culture dish and the C culture dish and containing high-concentration formaldehyde respectively after 0.12% the formalin degerming with mass concentration, every ware adds 1mL;
With the purification bacterial classification inoculation of described step (1) preparation in the described B culture medium that contains low concentration formaldehyde 28 ℃ cultivated 3 days, being transferred in the described C culture medium that contains low concentration formaldehyde 28 ℃ cultivated 3 days, being transferred in described containing in the B culture medium of concentration formaldehyde 28 ℃ again cultivated 3 days, being transferred in described containing in the C culture medium of concentration formaldehyde 28 ℃ again cultivated 3 days, being transferred in the described B culture medium that contains high-concentration formaldehyde 28 ℃ again cultivated 3 days, being transferred in the described C culture medium that contains high-concentration formaldehyde 28 ℃ again cultivated 3 days, being transferred in the described A culture medium 28 ℃ again cultivated 2 days, reach exponential phase, get bacterium colony and make solid agar slant preservation strain, cultivate after 2 days for 28 ℃, the thalline of having been tamed is preserved stand-by in 4 ℃ of refrigerators;
(4) with calcium alginate embedded thalline of having tamed: with embodiment 7.
Embodiment 13
Clean-up effect: the material for air purification of getting the microorganism absorption degradation of embodiment 7-12 preparation is loaded in the container of bottom band mesh, and can place the haydite that particle diameter is 18-25mm at container bottom, after container placed the air conditioning system humidifier section, air-flow behind the humidification is forced by container, thereby made gas forced flow container.When the air behind the humidification when the bottom mesh evenly flows through the gas forced flow container of the material for air purification that microorganism absorption degradation is housed, pollutant will be adsorbed by biologic grain and degrade, its clean-up effect is as described below:
Be provided with in the Laboratory Module of circulation supply and exhaust device at a volume 0.8 * 0.8 * 1, the initial concentration of formaldehyde and benzene series thing is respectively 0.7mg/m in the cabin
3And 0.8g/m
3When not adding the material for air purification of microorganism absorption degradation, the decay of the concentration of Laboratory Module internal contamination thing was not slowly reduced to 0.1mg/m yet in 12 hours
3And 0.11mg/m
3(0.1mg/m
3And 0.11mg/m
3Be respectively national standard to indoor formaldehyde and benzene series thing release value set upper limit concentration).And after the gas forced flow container of material for air purification that will be filled with the microorganism absorption degradation of embodiment 7-12 preparation placed in the Laboratory Module, the concentration decay of cabin internal contamination thing was very fast, and concentration of formaldehyde both can be reduced to 0.1mg/m in the cabin in 9 hours
3Below, the benzene series substrate concentration can be reduced to 0.11mg/m behind the 10h simultaneously
3Below.For exceed standard 100% room of formaldehyde in the reality building and benzene series substrate concentration (is that formaldehyde and benzene series substrate concentration are respectively 0.2mg/m in the room
3With 0.22～0.25mg/m
3), utilize the material for air purification of microorganism absorption degradation remove in the room formaldehyde and benzene series thing extremely healthy concentration range only need 4-5h.Fig. 4 has characterized the situation of concentration of formaldehyde decay in the Laboratory Module.
Claims (8)
1. the material for air purification of a microorganism absorption degradation is characterized in that the calcium alginate gel embedding through intersecting the particle diameter of Rhodopseudomonas after cultivating domestication or acinetobacter at the biologic grain of 2～4mm.
2. the material for air purification of a kind of microorganism absorption degradation according to claim 1 is characterized in that described Rhodopseudomonas thalline is pseudomonas pseudoalcaligenes Pseudomonas pseudoalcaligenes (CGMCC1.31), Pseudomonas aeruginosa Pseudomonas.A eruginosa (CGMCC 1.1129), pseudomonas putida Pseudomonas putida (CGMCC1.1130), pseudomonas fluorescens Pseudomonas fluorescens (CICC10271) or pseudomonas testosteroni Pseudomonas testosteroni (CICC20542).
3. the material for air purification of a kind of microorganism absorption degradation according to claim 1 is characterized in that described acinetobacter thalline is acinetobacter calcoaceticus Acinetobacter calcoaceticus:(CGMCC 1.2004).
4. the preparation method of the material for air purification of a microorganism absorption degradation is characterized in that comprising the steps:
(1) Rhodopseudomonas or acinetobacter activated with purification cultivate:
(2) preparation A culture medium, B culture medium and C culture medium:
Described A culture medium is full Nutrient medium, and pH is 7.0, consists of:
Described B culture medium is the sugar-free mineral salts medium, and concentration is g/L, consists of:
Solvent is a distilled water;
Described C culture medium is the sugaring mineral salts medium, is that the adding glucose is formulated in the B culture medium, and the concentration of described glucose is 0.37g/L;
(3) Rhodopseudomonas or acinetobacter intersected cultivate domestication:
A culture medium, B culture medium and the C culture medium of step (2) preparation are put into culture dish respectively, make the 15-30mL/ ware;
Be to join the C culture medium of making the B culture medium that contains low concentration formaldehyde in B culture dish and the C culture dish and containing low concentration formaldehyde respectively after 0.01%～0.03% the formalin degerming with mass concentration, every ware adds 1mL;
With mass concentration be join respectively after 0.02%～0.06% the formalin degerming make in B culture dish and the C culture dish contain in concentration formaldehyde the B culture medium and contain in the C culture medium of concentration formaldehyde, every ware adds 1mL;
Be to join the C culture medium of making the B culture medium that contains high-concentration formaldehyde in B culture dish and the C culture dish and containing high-concentration formaldehyde respectively after 0.04～0.12% the formalin degerming with mass concentration, every ware adds 1mL;
With the purification bacterial classification inoculation of described step (1) preparation in the described B culture medium that contains low concentration formaldehyde 25～35 ℃ cultivate 2～3 days after, be transferred in the described C culture medium that contains low concentration formaldehyde 25～35 ℃ and cultivated 2～3 days; Then be transferred in described containing in the B culture medium of concentration formaldehyde 25～35 ℃ and cultivate after 2～3 days, be transferred in the C culture medium of concentration formaldehyde in described containing 25～35 ℃ and cultivated 2～3 days; Being transferred in the described B culture medium that contains high-concentration formaldehyde 25～35 ℃ afterwards cultivated 2～3 days, being transferred in the described C culture medium that contains high-concentration formaldehyde 25～35 ℃ again cultivated 2～3 days, being transferred in the described A culture medium 25～35 ℃ at last cultivated 1～2 day, reach exponential phase or stable phase, get bacterium colony and make solid agar slant preservation strain, cultivate after 1～2 day for 25 ℃～35 ℃, the thalline of having been tamed is preserved stand-by in 4 ℃ of refrigerators;
(4) with calcium alginate embedded thalline of having tamed:
The thalline of having tamed of step (3) preparation is inoculated in the triangular flask that the sterilized nutrient broth fluid medium of 500mL is housed, under 25 ℃～35 ℃ on shaking table constant temperature culture 1～2 day, cell density reaches 10
9-10
10Individual/mL, get bacteria suspension, get the centrifuge tube that the 50mL bacteria suspension injects sterilization, separated 15-30 minute under 4000rpm, separating obtained somatic cells is through sterile saline washing 2～3 times, and adding distil water is to 50mL, somatic cells is suspended in the distilled water, must adds the water bacteria suspension;
Be 100: 0.5～1 ratio by volume with mass concentration be the sodium alginate aqueous solution of 2%-5% and describedly make mixing material after adding water bacteria suspension mixing, described mixing material is splashed into the CaCl that mass concentration is 5%-10% with the aciculiform pipe
2In the aqueous solution, left standstill 7～8 hours, obtain the granule of 2～5mm, leach behind the granule and clean, make a kind of biological absorbed air scavenging material with normal saline and distilled water.
5. the preparation method of the material for air purification of microorganism absorption degradation according to claim 4 is characterized in that described step (1) is:
Get Rhodopseudomonas or acinetobacter thalline in the lyophilisation pipe, under gnotobasis, inject the nutrient broth fluid medium of 80-120mL, in 1～2 day activated spawn of 25～35 ℃ of following continuous culture of shaking table, utilize the plate streaking partition method to isolate single bacterium colony, continuous culture is 1～2 day in 25～35 ℃ of constant incubators, being transferred to the solid agar slant culture-medium cultivated 1～2 day, in 4 ℃ of refrigerators, preserve stand-by, consisting of of described nutrient broth fluid medium: Carnis Bovis seu Bubali cream 1.5g, peptone 1g, NaCl1.5g, distilled water 100mL; Consisting of of described solid agar slant culture-medium: nutrient broth fluid medium 100mL, agar 4-5g, adjusting pH is 7-7.5.
6. according to the preparation method of the material for air purification of right 4 or 5 described a kind of microorganism absorption degradations, it is characterized in that described Rhodopseudomonas thalline is pseudomonas pseudoalcaligenes Pseudomonas pseudoalcaligenes (CGMCC1.31), Pseudomonas aeruginosa Pseudomonas.Aeruginosa (CGMCC1.1129), pseudomonas putida Pseudomonas putida (CGMCC1.1130), pseudomonas fluorescens Pseudomonas fluorescens (CICC10271) or pseudomonas testosteroni Pseudomonas testosteroni (CICC20542).
7. according to the preparation method of the material for air purification of right 4 or 5 described a kind of microorganism absorption degradations, it is characterized in that described acinetobacter thalline is acinetobacter calcoaceticus Acinetobacter calcoaceticus (CGMCC 1.2004).
8. the application of the material for air purification of the microorganism absorption degradation of one of claim 1 to 3 in absorption degradation aerochemistry pollutant.
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