CN101544949A - Method for improving fermentation yield of water soluble polymer - Google Patents
Method for improving fermentation yield of water soluble polymer Download PDFInfo
- Publication number
- CN101544949A CN101544949A CN200910068715A CN200910068715A CN101544949A CN 101544949 A CN101544949 A CN 101544949A CN 200910068715 A CN200910068715 A CN 200910068715A CN 200910068715 A CN200910068715 A CN 200910068715A CN 101544949 A CN101544949 A CN 101544949A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- thalline
- polymkeric substance
- original position
- water soluble
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for improving the fermentation yield of a water soluble polymer, and relates to an in-situ separation fermentation system formed by coupling a fermentation device and an ultra-filtration separation device. While the water soluble polymer is produced by fermentation, in-situ separation is realized, which is to ferment while separating the polymer. When fermentation is carried out to 1/10-1/5 of a fermentation period, the in-situ separation fermentation system is started to operate every 1/10-1/6 of the fermentation period for 2 to 3 hours each time. The technology can ensure mass transmission value of dissolved oxygen of fermentation liquid. Under the condition of the equivalent inventory rating, compared with the water soluble polymer produced by common fermentation, the method can improve the yield by 30 to 80 percent.
Description
Technical field
The present invention relates to a kind of method that improves fermentation yield of water soluble polymer.Belong to the industrial biotechnology field.
Technical background
Along with the continuous development of science and technology, the related field of microbial fermentation product is more and more wider.When producing the microbial fermentation product, one of problem that often runs into is that product suppresses growth, breeding or the metabolism of microorganism itself, and fermentation can't normally be carried out when the tunning accumulation reaches finite concentration.As: lactic fermentation.In the fermentative production of water soluble polymer, be accompanied by the carrying out of fermentation, water soluble polymer constantly accumulates, and makes the viscosity of fermented liquid strengthen, and has a strong impact on the dissolved oxygen mass transfer effect, also makes fermentation be difficult to normally carry out, and product yield is low.In order to address the above problem, people put forth effort on research and development original position product separate fermentation technology---the complex art that a kind of original position product separates and fermentation organically combines.(in situ product removal ISPR) is meant the method that the meta-bolites of producing cell is removed fast to the separation of original position product.Recently, people have researched and developed many kinds of original position separate fermentation technology, comprise: solvent extraction fermentation method (selecting the transitivity extraction agent according to the dissolution characteristics of product), (fermentation system comprises the resin absorption device to the absorption fermentation method, select sorbent materials such as specificity ion exchange resin according to product property), (fermentation system comprises electrodialysis unit to the electrodialysis fermentation method, rely on product electrically charged character in fermented liquid, product is moved to the concentrated solution hold tank from nutrient solution).As: the Li Tao of University Of Science and Technology Of Tianjin etc. are with Inorganic Ceramic Membranes and the coupling of Xie Shi fermentation using propionibacterium, made up a kind of novel original position separate fermentation system, by charcoal absorption, successfully the propionic acid in the fermentation system is separated, effectively removed the restraining effect of propionic acid, realized the high density fermentation of vitamin B12 (VB12) thalli growth; Xavier etc. influence lactic acid-producing to tubular ultra-filtration membrane cell cycle bio-reactor and study, and have obtained the ideal experimental result, can obtain not celliferous clarified broth, have higher lactic acid yield and product concentration; Danner etc. have designed ultrafiltration membrane bioreactor (MBR)-one pole electrodialysis case (ED), constitute MBR-ED unit operation system, continuously ferment and produce the research of lactic acid from compost, screening the stearothermophilus bud robe bacillus BS 119 that can utilize hexose and pentose.MBR-ED unit operation system makes lactic acid concn reach 115g/L, behind operation 1052h, to its mensuration, has not measured microbial contamination, and can also cut down the consumption of energy, and reduces the discharging of high biological oxygen demand (BOD) (BOD) waste water, and very high environmental benefit is arranged.The Xu Zhi of Zhejiang University south study group adopts rotation fibre bed immobilized cell technology in the fermentative production of water soluble polymer, has effectively improved the productive rate of polyglutamic acid.
Summary of the invention
The technical problem that solves
The purpose of this invention is to provide a kind of method that improves fermentation yield of water soluble polymer.
Technical scheme
Technical scheme of the present invention is: with fermentation unit and ultra-filtration equipment mutually coupling become original position separate fermentation system.This original position separate fermentation system mainly comprises: (1) fermentation unit, (2) constant flow pump, (3) thalline filtration unit, (4) constant flow pump, (5) polymer filter, (6) polymkeric substance storage vat, (7) fluid infusion bucket, (8) fermented liquid flows out pipe, (9) thalline return line, (10) no thalline fermentation broth stream goes out pipe, and (11) polymkeric substance flows out pipe, and (12) no thalline does not have polymkeric substance fermented liquid return line.
Original position separate fermentation system operation mode involved in the present invention is: the original position that brings into operation when fermentation proceeds to 1/10-1/5 fermentation period separate fermentation system, each run 2-3 hour; Each 1/10-1/6 fermentation period at interval.
The concrete operations operation scheme of this original position separate fermentation system is: start constant flow pump (2), fermented liquid enters thalline filtration unit (3) (external-compression type ultra-filtration equipment, select suitable tubular fibre filtering membrane aperture according to the microorganism size, as: bacterium is selected 0.2 μ), implement ultrafiltration for the first time, thalline is back to fermentation unit (1) by return line (9), no thalline fermented liquid enters polymer filter (5) (selecting the strainer in suitable aperture according to the size of polymericular weight) by constant flow pump (4), polymkeric substance enters polymkeric substance storage vat (6) by outlet pipe (11), and no thalline does not have the polymkeric substance fermented liquid and enters fermentation unit (1) by return line (12); Mend sterilized water in the fluid infusion bucket (7) to fermentation unit (1), the control amount of mending equals the amount of liquid in the polymkeric substance storage vat (6).
Beneficial effect
Original position separate fermentation method of the present invention is produced water soluble polymer, in the fermentative production water soluble polymer, realizes that the original position of product is separated, and carries out the separation of polymkeric substance while fermenting that is:.Can guarantee the dissolved oxygen of fermentation liquid mass transfer effect.Under the situation of equal charging capacity, the productive rate that adopts this technology and common fermentation to produce is compared, and can improve the productive rate 30%-80% of water soluble polymer.
Description of drawings
Accompanying drawing is the original position separate fermentation system schematic that microbial fermentation is produced water soluble polymer
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
(Aureobasidium pullulan, IFO6353) fermentation method prepares water soluble polymer-----Beta-polymalic acid [Poly (β-malic acid), β-PMA] to adopt Aureobasidium pullulans.
Concrete operations: with Aureobasidium pullulan, the IFO6353 freeze-drying lactobacillus adopts the PSA substratum to activate; Carrying out seed liquor cultivates; Seed liquor is inoculated in the 10.0L fermention medium with the inoculum size of 1%-3% ferments; The common fermentation condition of all fermentation conditions and Beta-polymalic acid is consistent, as: temperature, rotating speed, air flow etc.It is the filter membrane of 0.2 μ that the thalline filter is selected the aperture, and it is 15,000 that the superpolymer filter is selected the molecular weight cutoff value.After fermentation is carried out 96 hours, the original position that brings into operation separate fermentation system, each run 3 hours, operation is spaced apart 20 hours, and fermentation period 200 hours moves four times altogether.After the fermentation ends, solid-liquid separation is carried out ethanol sedimentation, removes Propiram, refining Beta-polymalic acid.Productive rate is compared with common fermentation and has been improved 80%.
Adopt bacillus licheniformis (Bacillus licheniformis NK-01) fermentation method to prepare water soluble polymer-----gamma-polyglutamic acid-[Poly (γ-gulutamic acid), γ-PGA].
Concrete operations: adopt the LB substratum to activate Bacillus licheniformis NK-01 freeze-drying lactobacillus; Carrying out seed liquor cultivates; Seed liquor is inoculated in the 10.0L fermention medium with the inoculum size of 1%-3% ferments; The common fermentation condition of all fermentation conditions and gamma-polyglutamic acid-is consistent, as: temperature, rotating speed, air flow etc.It is the filter membrane of 0.2 μ that the thalline filter is selected the aperture, and the polymkeric substance filter is selected molecular weight cutoff value 50,000.After fermentation is carried out 24 hours, the original position that brings into operation separate fermentation system, each run 2 hours, operation is spaced apart 12 hours, and fermentation period 96 hours moves six times altogether.After the fermentation ends, solid-liquid separation is carried out ethanol sedimentation, refining gamma-polyglutamic acid-.Productive rate is compared with common fermentation and has been improved 50%.
(Aureobasidium pullulan, IFO6353) fermentation method prepares water soluble polymer-----Propiram (pullulan) to adopt Aureobasidium pullulans.
Concrete operations: with Aureobasidium pullulan, the IFO6353 freeze-drying lactobacillus adopts the PSA substratum to activate; Carrying out seed liquor cultivates; Seed liquor is inoculated in the 10.0L fermention medium with the inoculum size of 1%-3% ferments; The common fermentation condition of all fermentation conditions and Propiram is consistent, as: temperature, rotating speed, air flow etc.It is the filter membrane of 0.2 μ that the thalline filter is selected the aperture, and the polymkeric substance filter is selected molecular weight cutoff value 20,000.After fermentation is carried out 96 hours, the original position that brings into operation separate fermentation system, each run 2 hours, operation is spaced apart 24 hours, and fermentation period 168 hours moves twice altogether.After the fermentation ends, solid-liquid separation is carried out ethanol sedimentation, refining Propiram.Productive rate is compared with common fermentation and has been improved 30%.
Claims (4)
1. method that improves fermentation yield of water soluble polymer, it is characterized in that with fermentation unit and ultra-filtration equipment mutually coupling become original position separate fermentation system.
2. method according to claim 1 is characterized in that, this original position separate fermentation system mainly comprises: (1) fermentation unit, (2) constant flow pump, (3) thalline filtration unit, (4) constant flow pump, (5) polymer filter, (6) polymkeric substance storage vat, (7) fluid infusion bucket, (8) fermented liquid flows out pipe, (9) thalline return line, (10) no thalline fermentation broth stream goes out pipe, and (11) polymkeric substance flows out pipe, and (12) no thalline does not have polymkeric substance fermented liquid return line.
3. method according to claim 1 is characterized in that, the original position separate fermentation system operation mode that present method relates to is: the original position that brings into operation when fermentation proceeds to 1/10-1/5 fermentation period separate fermentation system, each run 2-3 hour; Each 1/10-1/6 fermentation period at interval.
4. according to claim 1 or 2 or 3 described methods, it is characterized in that, the concrete operations operation scheme of this original position separate fermentation system is: start constant flow pump (2), fermented liquid enters thalline filtration unit (3), implement ultrafiltration for the first time, thalline is back to fermentation unit (1) by return line (9), no thalline fermented liquid enters polymer filter (5) by constant flow pump (4), polymkeric substance enters polymkeric substance storage vat (6) by outlet pipe (11), and no thalline does not have the polymkeric substance fermented liquid and enters fermentation unit (1) by return line (12); Mend sterilized water in the fluid infusion bucket (7) to fermentation unit (1), the control amount of mending equals the amount of liquid in the polymkeric substance storage vat (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910068715A CN101544949A (en) | 2009-04-30 | 2009-04-30 | Method for improving fermentation yield of water soluble polymer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910068715A CN101544949A (en) | 2009-04-30 | 2009-04-30 | Method for improving fermentation yield of water soluble polymer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101544949A true CN101544949A (en) | 2009-09-30 |
Family
ID=41192327
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910068715A Pending CN101544949A (en) | 2009-04-30 | 2009-04-30 | Method for improving fermentation yield of water soluble polymer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101544949A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154408A (en) * | 2011-01-13 | 2011-08-17 | 天津市工业微生物研究所 | Sclerotium rolfssii scleroglucan online fermentation extraction method and system |
| CN103045662A (en) * | 2012-12-31 | 2013-04-17 | 天津北洋百川生物技术有限公司 | Fermentation medium for improving output and purity of beta-polymalic acid produced by zymotechnics |
| CN104004803A (en) * | 2014-05-22 | 2014-08-27 | 河北科技大学 | Method for producing antimicrobial peptide through fermentation of brevibacillus laterosporu |
-
2009
- 2009-04-30 CN CN200910068715A patent/CN101544949A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154408A (en) * | 2011-01-13 | 2011-08-17 | 天津市工业微生物研究所 | Sclerotium rolfssii scleroglucan online fermentation extraction method and system |
| CN103045662A (en) * | 2012-12-31 | 2013-04-17 | 天津北洋百川生物技术有限公司 | Fermentation medium for improving output and purity of beta-polymalic acid produced by zymotechnics |
| CN104004803A (en) * | 2014-05-22 | 2014-08-27 | 河北科技大学 | Method for producing antimicrobial peptide through fermentation of brevibacillus laterosporu |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Liao et al. | A review of membrane fouling and its control in algal-related membrane processes | |
| Wang et al. | Development of a novel anaerobic membrane bioreactor simultaneously integrating microfiltration and forward osmosis membranes for low-strength wastewater treatment | |
| Oshiki et al. | Cultivation of planktonic anaerobic ammonium oxidation (anammox) bacteria using membrane bioreactor | |
| CN103865792B (en) | A kind of circulating fermentable reaction and feed liquid isolation integral equipment | |
| CN101838342B (en) | Membrane separation method for microalgae extracellular polysaccharide | |
| Wang et al. | Pollutant removal and membrane fouling in an anaerobic submerged membrane bioreactor for real sewage treatment | |
| CN101254969B (en) | Method for preparing microbial flocculant by using byproduct bacterial of fermentation industry | |
| Gao et al. | Dynamic variation of microbial metabolites and community involved in membrane fouling in A/O-MBR | |
| CN103073652A (en) | Method for extracting polysaccharide of spirulina platensis | |
| CN104649519A (en) | Sewage treatment device and method by combining bio-ferric process and anaerobic MBR method | |
| Matsumoto et al. | Influence of extracellular polysaccharides (EPS) produced by two different green unicellular algae on membrane filtration in an algae-based biofuel production process | |
| CN101544949A (en) | Method for improving fermentation yield of water soluble polymer | |
| CN101586074B (en) | Algae cultivation system with sewage | |
| CN102838624A (en) | Method for purifying clavulanic acid from fermentation liquor | |
| CN103740775B (en) | The method of recycle schizochytrium limacinum fermentation clear liquid production DHA grease | |
| Sivagurunathan et al. | High-rate hydrogen production from galactose in an upflow anaerobic sludge blanket reactor (UASBr) | |
| CN103103125B (en) | A kind of microalgae recovery method that microbial flocculation and air supporting are coupled | |
| Du et al. | Characteristics of aerobic granular sludge cultivated by promoting and inhibiting filamentous bacteria | |
| CN102372624B (en) | Method for extracting sodium gulonate from sodium gulonate fermented liquid | |
| CN115367977A (en) | Method for realizing sludge reduction and carbon emission reduction by optimizing and redistributing carbon source in sewage plant | |
| CN105039139B (en) | A kind of microculture film and microculture recovery system | |
| CN104478181B (en) | Sludge reduction device and method combining composite enzyme treatment with microfauna predation | |
| CN211394245U (en) | Anaerobic fermentation liquor treatment equipment for livestock manure | |
| CN1861781A (en) | Picking method of single cell algae | |
| CN102863433A (en) | Mupirocin purification method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20090930 |