CN101530099A - Isothiazolone-containing bactericidal composition - Google Patents
Isothiazolone-containing bactericidal composition Download PDFInfo
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- CN101530099A CN101530099A CN200910022208A CN200910022208A CN101530099A CN 101530099 A CN101530099 A CN 101530099A CN 200910022208 A CN200910022208 A CN 200910022208A CN 200910022208 A CN200910022208 A CN 200910022208A CN 101530099 A CN101530099 A CN 101530099A
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Abstract
The invention discloses an isothiazolone-containing bactericidal composition and application thereof, and in particular relates to a bactericidal composition containing isothiazolone as a first active component and amino-oligosaccharin as a second active component, and application thereof. The weight ratio of the isothiazolone to the amino-oligosaccharin in the composition is 0.01-140:1, preferably 0.05-5:1. The composition has the advantages that the composition is reasonable in components, good in bactericidal effects and low in drug-using cost; the activity and bactericidal effects of the composition have remarkable synergy instead of simply adding the activity of each component; and the composition is quick in response and long in lasting effects, slows down resistance generation, has good safety to crops, and meets the requirement of pesticide preparations on safety. The prevention and control effect of the composition on the crops diseases of fruit trees, grain, melons, fruits and vegetables reaches 80.28 to 91.65 percent.
Description
Technical field
The present invention relates to a kind of bactericidal composition of isothiazolone-containing, be specifically related to a kind of bactericidal composition that contains the first active component isothiazolinone and the second active component amino-oligosaccharide.
Background technology
Isothiazolinone is made up of 5-chloro-2-methyl-4-isothiazoline-3-ketone (CMI) and 2-methyl-4-isothiazoline-3-ketone (MI), trade name: CIT/MIT, triumphant pine, card pine, Kathon, isothiazolinone, CAS NO:26172-55-4/2682-20-4, molecular formula: C
4H
5NOS/C
4H
4ClNOS, molecular weight: 115.16/149.59.
Isothiazolinone is a kind of wide spectrum, efficient, low toxicity, non-oxidative bactericide, works to kill livestock acting on by the key that disconnects bacterium and algae protein matter.Isothiazolinone can promptly irreversibly suppress its growth with after microorganism contacts, thereby causes the death of microbial cell, and common bacteria, fungi, algae etc. are had very strong inhibition and killing action.The efficient of killing livestock height, degradability is good, has residual, handling safety, the characteristics that stability is strong of not producing.The isothiazolinone controlling plant diseases is not appeared in the newspapers, show that through Shanggezhilu Biosciences Co., Ltd., Shaanxi's field control effectiveness test isothiazolinone has the better prevention effect to diseases such as paddy rice bacterial leaf spot, apple valsa cankers, but the isothiazolinone lasting period is shorter, causes the medication number of times more.Isothiazolinone reasonably is mixed, can effectively brings into play its bactericidal action, prolong the lasting period, delay the generation of germ resistance, obtain better protection effect.
Amino-oligosaccharide english common name oligosaccharins, molecular formula (C
6H
11O
4N) n (n 〉=2), a kind of bactericide that the metabolism of amino-oligosaccharide microorganism belonging to genus is extracted with resistant effect, pathogen growth there is inhibitory action, can influence fungus spore germination, bringing out the mycelia form morphs, biochemical reaction changes etc. in the born of the same parents of mycelia, and the mechanism that inducing plant produces disease resistance mainly is to excite gene expression in plants, produces chitinase, dextranase, guarantor's element and PR albumen etc. with antibacterial action; Have the gene expression that suppresses germ simultaneously, the Physiology and biochemistry of mycelia is morphed, growth is suppressed, simultaneously can also the stimulating plant growth.Amino-oligosacchride exists in actual use because effect is slower, to the dissatisfactory defective of the diseases control that has infected.
At present, the isothiazolinone controlling plant diseases is not appeared in the newspapers, through Shanggezhilu Biosciences Co., Ltd., Shaanxi's experimental study, discovery has better prevention effect and the good characteristics of quick-acting to diseases such as paddy rice bacterial leaf spot, apple valsa cankers, but exist the lasting period shorter, the medication number of times is more, the defective that use cost is high; Amino-oligosaccharide has inducing plant and produces antibody, the stimulating plant growth, and the advantage that the lasting period is long, but exist quick-acting slow, to the dissatisfactory defective of the diseases control that has infected.The inventor is unexpected to be found the use that is mixed of isothiazolinone and amino-oligosaccharide can be improved its quick-acting, and the good lasting period is arranged again, slow down the generation of disease drug resistance, and use cost is low.
Summary of the invention
The object of the present invention is to provide a kind of component reasonable, bactericidal effect is good, and drug cost is low, the bactericidal composition of a kind of isothiazolone-containing that is difficult for developing immunity to drugs.
Another object of the present invention is to provide the bactericidal composition preparation formulation of isothiazolone-containing.
A further object of the present invention is to provide the application of the bactericidal composition of isothiazolone-containing.
In order to overcome the defective of existing unitary agent, technical scheme of the present invention is to solve like this:
A kind of bactericidal composition of isothiazolone-containing is characterized in that comprising:
A) the first active component isothiazolinone;
B) the second active component amino-oligosaccharide;
The weight ratio of first active component and second active component is 0.01～140:1, and suboptimum is elected 0.02～60:1 as, is preferably 0.04～30:1, most preferably is 0.05～5:1.
The bactericidal composition of isothiazolone-containing of the present invention can be mixed with aqua.For the aqua formulation, those skilled in the art are familiar with the use corresponding adjuvants and finish the present invention very much.Emulsifier such as calcium dodecyl benzene sulfonate (agricultural newborn 500#), Nongru-700 # (common name: alkyl phenol formaldehyde resin polyoxyethylene ether), the newborn 2201# of farming, Span-60# (common name: sorbitan monostearate), Tween-60# (common name: the sorbitan monostearate APEO), TX-10 (common name: octyl phenol polyoxy ethene (10) ether), penetrating agent JFC (fatty alcohol-polyoxyethylene ether), the newborn 1601# of farming (common name: phenethyl phenol polyoxyethylene poly-oxygen propylene aether), the newborn 600# of farming, the newborn 400# of farming, in the dodecyl benzyl dimethyl ammonium chloride one or more, water is deionized water.
The present invention compared with prior art, adopt two kinds of active component combinations, this composition is reasonable, good antimicrobial effect, and its activity and fungistatic effect are not the simple superposition of each composition activity, but significant synergistic effect is arranged, and quick-acting is good, the lasting period is long, reduced drug cost, the present invention has 80.28%～91.65% control efficiency to fruit tree, cereal class, melon and fruit class, greengrocery crop pest.
Embodiment
Below in conjunction with embodiment content of the present invention is described further.
Example of formulations 1:
Take by weighing 12% isothiazolinone, 0.1% amino-oligosaccharide, 3%TX-10, deionized water and add to 100% weight portion.Above-mentioned raw materials makes 12.1% isothiazolinone amino-oligosaccharide aqua after mix, stirring and dissolving is complete.
The part by weight of isothiazolinone and amino-oligosaccharide can change between 0.01～140:1 in the present embodiment, and both total weight items still are 12.1%, form new embodiment.
Example of formulations 2:
Take by weighing 10% isothiazolinone, 0.2% amino-oligosaccharide, 0.5% agricultural newborn 500#, 3% Nongru-700 #, deionized water and add to 100% weight portion.Above-mentioned raw materials makes 10.2% isothiazolinone amino-oligosaccharide aqua after mix, stirring and dissolving is complete.
The part by weight of isothiazolinone and amino-oligosaccharide can change between 0.01～140:1 in the present embodiment, and both total weight items still are 10.2%, form new embodiment.
Example of formulations 3:
Take by weighing 8% isothiazolinone, 0.5% amino-oligosaccharide, 3% penetrating agent JFC, deionized water and add to 100% weight portion.Above-mentioned raw materials makes 10.2% isothiazolinone amino-oligosaccharide aqua after mix, stirring and dissolving is complete.
The part by weight of isothiazolinone and amino-oligosaccharide can change between 0.01～140:1 in the present embodiment, and both total weight items still are 8.5%, form new embodiment.
Example of formulations 4:
Take by weighing 0.25% isothiazolinone, 0.25% amino-oligosaccharide, 3% penetrating agent JFC, deionized water and add to 100% weight portion.Above-mentioned raw materials makes 0.5% isothiazolinone amino-oligosaccharide aqua after mix, stirring and dissolving is complete.
The part by weight of isothiazolinone and amino-oligosaccharide can change between 0.01～140:1 in the present embodiment, and both total weight items still are 0.5%, form new embodiment.
Example of formulations 5:
Take by weighing 0.5% isothiazolinone, 30% amino-oligosaccharide, 0.3% agricultural newborn 500#, 3%Tween-60#, deionized water and add to 100% weight portion.Above-mentioned raw materials makes 30.5% isothiazolinone amino-oligosaccharide aqua after mix, stirring and dissolving is complete.
The part by weight of isothiazolinone and amino-oligosaccharide can change between 0.01～140:1 in the present embodiment, and both total weight items still are 30.5%, form new embodiment.
Example of formulations 6:
Take by weighing 0.66% isothiazolinone, 0.34% amino-oligosaccharide, 3% penetrating agent JFC, 3% dodecyl benzyl dimethyl ammonium chloride, deionized water and add to 100% weight portion.Above-mentioned raw materials makes 1% isothiazolinone amino-oligosaccharide aqua after mix, stirring and dissolving is complete.
The part by weight of isothiazolinone and amino-oligosaccharide can change between 0.01～140:1 in the present embodiment, and both total weight items still are 1%, form new embodiment.
Example of formulations 7:
Take by weighing 0.5% isothiazolinone, 15% amino-oligosaccharide, 1% Span-60#, 3% dodecyl benzyl dimethyl ammonium chloride, deionized water and add to 100% weight portion.Above-mentioned raw materials makes 15.5% isothiazolinone amino-oligosaccharide aqua after mix, stirring and dissolving is complete.
The part by weight of isothiazolinone and amino-oligosaccharide can change between 0.01～140:1 in the present embodiment, and both total weight items still are 15.5%, form new embodiment.
Biological Examples 1 isothiazolinone and amino-oligosaccharide are mixed to cucumber downy mildew germ synergy Toxicity Determination
Adopt indoor pot experiment, be designated as standard (NY/T1154.4-2006) with People's Republic of China's agricultural every trade, carried out isothiazolinone and amino-oligosaccharide and be mixed to the synergy indoor virulence experiment of cucumber downy mildew, the result is as follows:
The single agent of table 1 isothiazolinone is to the control efficiency of cucumber downy mildew
The single agent of medicament isothiazolinone
Concentration (ug/ml) 0.78 1.56 3.125 6.25 12.5
Average control efficiency (%) 43.75 60.88 71.26 87.55 93.27
Virulence regression equation Y=4.98557+1.35336X
Correlation R=0.9900
The standard error SE (B) 0.30509 of slope
EC
50(ug/ml) 1.02486
EC
5095% confidence interval 0.80956～1.21854
EC
50(ug/ml) 9.06992
EC
9095% confidence interval 6.44996～14.28767
The agent of table 2 amino-oligosaccharide list is to the control efficiency of cucumber downy mildew
The agent of medicament amino-oligosaccharide list
Concentration (ug/ml) 6.25 12.5 25 40 80
Average control efficiency (%) 20.69 37.88 49.52 65.79 83.21
Virulence regression equation Y=2.94291+1.53785X
Correlation R=0.9938
The standard error SE (B) 0.34895 of slope
EC
50(ug/ml) 21.7588
EC
5095% confidence interval 12.11～53.30
EC
50(ug/ml) 148.2425
EC
9095% confidence interval 59.1809～614.18
Table 3 (1) isothiazolinone and amino-oligosaccharide are mixed to the control efficiency of cucumber downy mildew
Medicament isothiazolinone: amino-oligosaccharide (0.1:2)
Concentration (ug/ml) 0.78 1.56 3.125 6.25 12.5
Average control efficiency (%) 16.69 24.87 35.55 49.67 69.99
Virulence regression equation Y=4.06614+1.24767X
Correlation R=0.9922
The standard error SE (B) 0.32536 of slope
EC
50(ug/ml) 5.60337
EC
5095% confidence interval 3.7377～10.3349
EC
50(ug/ml) 59.6533
EC
9095% confidence interval 26.2909～221.2408
Coefficient of synergism (SR) 1.97
(2) isothiazolinone and amino-oligosaccharide are mixed to the control efficiency of cucumber downy mildew
Medicament isothiazolinone: amino-oligosaccharide (0.3:1.7)
Concentration (ug/ml) 0.78 1.56 3.125 6.25 12.5
Average control efficiency (%) 21.45 34.16 57.85 70.01 85.79
Virulence regression equation Y=4.35353+1.5467X
Correlation R=0.9964
The standard error SE (B) 0.30981 of slope
EC
50(ug/ml) 2.6180
EC
5095% confidence interval 2.11749～3.40889
EC
50(ug/ml) 17.64
EC
9095% confidence interval 11.45927～31.69735
Coefficient of synergism (SR) 2.06
(3) isothiazolinone and amino-oligosaccharide are mixed to the control efficiency of cucumber downy mildew
Medicament isothiazolinone: amino-oligosaccharide (0.6:1.5)
Concentration (ug/ml) 0.78 1.56 3.125 6.25 12.5
Average control efficiency (%) 30.29 53.83 70.69 78.65 89.75
Virulence regression equation Y=4.7363+1.4672X
Correlation R=0.9907
The standard error SE (B) 0.38205 of slope
EC
50(ug/ml) 1.5127
EC
5095% confidence interval 1.1611～2.0767
EC
90(ug/ml) 11.3043
EC
9095% confidence interval 6.6469～26.1242
Coefficient of synergism (SR) 2.12
(4) isothiazolinone and amino-oligosaccharide are mixed to the control efficiency of cucumber downy mildew
Medicament isothiazolinone: amino-oligosaccharide (0.5:1)
Concentration (ug/ml) 0.78 1.56 3.125 6.25 12.5
Average control efficiency (%) 31.06 49.21 68.78 75.18 88.19
Virulence regression equation Y=4.70369+1.36504X
Correlation R=0.9923
The standard error SE (B) 0.34564 of slope
EC
50(ug/ml) 1.6484
EC
5095% confidence interval 1.2786～2.2478
EC
90(ug/ml) 14.3188
EC
9095% confidence interval 8.2885～33.3440
Coefficient of synergism (SR) 1.77
(5) isothiazolinone and amino-oligosaccharide are mixed to the control efficiency of cucumber downy mildew
Medicament isothiazolinone: amino-oligosaccharide (1:0.5)
Concentration (ug/ml) 0.78 1.56 3.125 6.25 12.5
Average control efficiency (%) 50.43 69.75 76.71 85.32 95.69
Virulence regression equation Y=5.18148+1.22081X
The standard error SE (B) 0.36853 of correlation R=0.98816 slope
EC
50(ug/ml) 0.7101
EC
5095% confidence interval 0.52353～0.92274
EC
90(ug/ml) 7.9634
EC
9095% confidence interval 4.9796～16.5248
Coefficient of synergism (SR) 2.11
(6) isothiazolinone and amino-oligosaccharide are mixed to the control efficiency of cucumber downy mildew
Medicament isothiazolinone: amino-oligosaccharide (1:1)
Concentration (ug/ml) 0.78 1.56 3.125 6.25 12.5
Average control efficiency (%) 52.55 69.80 78.55 89.27 98.99
Virulence regression equation Y=5.21229+1.35529X
The standard error SE (B) 0.36956 of correlation R=0.9914 slope
EC
50(ug/ml) 0.6972
EC
5095% confidence interval 0.5480～0.8619
EC
90(ug/ml) 6.1511
EC
9095% confidence interval 4.31392～10.1338
Coefficient of synergism (SR) 2.81
Result of the test shows: after isothiazolinone and amino-oligosaccharide two medicaments are mixed cucumber downy mildew is had obvious synergistic effect, mixed ratio isothiazolinone and amino-oligosaccharide are the most remarkable with 1:0.5 and 1:1 synergistic effect, and coefficient of synergism is respectively 2.11 and 2.81.
Biological Examples 2:
Adopt growth rate method, measure isothiazolinone and amino-oligosaccharide and be mixed the synergy indoor virulence experiment of tomato late blight germ
The single agent of table 4 isothiazolinone is to the inhibition effect of tomato late blight bacterium
The single agent of medicament isothiazolinone
Concentration (ug/ml) 0.39 0.78 1.56 3.125 6.25
Average inhibiting rate (%) 35.86 47.55 63.14 81.39 95.26
Virulence regression equation Y=5.02397+1.06393X
Correlation R=0.9755
The standard error SE (B) 0.41128 of slope
EC
50(ug/ml) 0.9494
EC
5095% put letter district 0.47701～1.845
Between
EC
50(ug/ml) 15.2448
EC
9095% put letter district 5.76609～141.1259
Between
The agent of table 5 amino-oligosaccharide list is to the inhibition effect of tomato late blight bacterium
The agent of medicament amino-oligosaccharide list
Concentration (ug/ml) 12.5 25 50 100 200
Average inhibiting rate (%) 23.55 39.86 53.89 75.69 87.88
Virulence regression equation Y=2.54837+1.55137X
Correlation R=0.9969
The standard error SE (B) 0.29910 of slope
EC
50(ug/ml) 38.0454
EC
5095% confidence interval 23.20～73.5565
EC
50(ug/ml) 254.9070
EC
9095% confidence interval 122.4618～681.9606
Table 6 (1) isothiazolinone and amino-oligosaccharide are mixed to the inhibition effect of tomato late blight bacterium
Medicament isothiazolinone: amino-oligosaccharide (0.1:2)
Concentration (ug/ml) 1.56 3.125 6.25 12.5 25
Average inhibiting rate (%) 18.69 35.32 46.81 58.45 70.66
Virulence regression equation Y=3.99796+1.12258X
Correlation R=0.9946
The standard error SE (B) 0.2778 of slope
EC
50(ug/ml) 7.8096
EC
5095% confidence interval 5.1658～14.2884
EC
50(ug/ml) 108.20
EC
9095% confidence interval 46.6886～387.3741
Coefficient of synergism (SR) 1.70
(2) isothiazolinone and amino-oligosaccharide are mixed to the inhibition effect of tomato late blight bacterium
Medicament isothiazolinone: amino-oligosaccharide (0.3:1.7)
Concentration (ug/ml) 1.56 3.125 6.25 12.5 25
Average inhibiting rate (%) 37.88 53.65 62.77 73.45 87.65
Virulence regression equation Y=4.4901+1.1039X
Correlation R=0.9928
The standard error SE (B) 0.2986 of slope
EC
50(ug/ml) 2.8971
EC
5095% confidence interval 2.1314～4.4524
EC
50(ug/ml) 41.9689
EC
9095% confidence interval 19.96～135.81
Coefficient of synergism (SR) 1.91
(3) isothiazolinone and amino-oligosaccharide are mixed to the inhibition effect of tomato late blight bacterium
Medicament isothiazolinone: amino-oligosaccharide (0.6:1.5)
Concentration (ug/ml) 1.56 3.125 6.25 12.5 25
Average inhibiting rate (%) 52.99 60.38 70.36 81.55 91.09
Virulence regression equation Y=4.8302+0.97202X
Correlation R=0.9884
The standard error SE (B) 0.31499 of slope
EC
50(ug/ml) 1.4953
EC
5095% confidence interval 1.11379～2.1418
EC
90(ug/ml) 31.1299
EC
9095% confidence interval 14.2447～118.2741
Coefficient of synergism (SR) 2.09
(4) isothiazolinone and amino-oligosaccharide are mixed to the inhibition effect of tomato late blight bacterium
Medicament isothiazolinone: amino-oligosaccharide (0.5:1)
Concentration (ug/ml) 1.56 3.125 6.25 12.5 25
Average inhibiting rate (%) 50.75 59.88 70.02 79.87 90.43
Virulence regression equation Y=4.7903+0.9819X
Correlation R=0.9929
The standard error SE (B) 0.2784 of slope
EC
50(ug/ml) 1.6351
EC
5095% confidence interval 1.2903～2.1726
EC
90(ug/ml) 33.0139
EC
9095% confidence interval 17.1967～88.8012
Coefficient of synergism (SR) 1.66
(5) isothiazolinone and amino-oligosaccharide are mixed to the inhibition effect of tomato late blight bacterium
Medicament isothiazolinone: amino-oligosaccharide (1:0.5)
Concentration (ug/ml) 1.56 3.125 6.25 12.5 25
Average inhibiting rate (%) 67.52 78.88 85.48 93.96 100
Virulence regression equation Y=5.20374+1.20286X
Correlation R=0.9888
The standard error SE (B) 0.3748 of slope
EC
50(ug/ml) 0.6744
EC
5095% confidence interval 0.54073～0.81972
EC
90(ug/ml) 7.8411
EC
9095% confidence interval 5.47565～12.8591
Coefficient of synergism (SR) 2.09
(6) isothiazolinone and amino-oligosaccharide are mixed to the inhibition effect of tomato late blight bacterium
Medicament isothiazolinone: amino-oligosaccharide (1:1)
Concentration (ug/ml) 1.56 3.125 6.25 12.5 25
Average inhibiting rate (%) 70.85 81.29 92.88 100 100
Virulence regression equation Y=5.21336+1.56227X
Correlation R=0.9878
The standard error SE (B) 0.46942 of slope
EC
50(ug/ml) 0.73081
EC
5095% confidence interval 0.61658～0.85334
EC
90(ug/ml) 4.82775
EC
9095% confidence interval 3.7775～6.5923
Coefficient of synergism (SR) 2.54
Result of the test shows: after isothiazolinone and amino-oligosaccharide two medicaments are mixed cucumber downy mildew is had obvious synergistic effect, the mixed ratio isothiazolinone: amino-oligosaccharide is the most remarkable with 1:0.5 and 1:1 synergistic effect, and coefficient of synergism is respectively 2.09 and 2.54.
Biological Examples 3 control cucumber downy mildew field trials
Carried out 0.5% isothiazolinone amino-oligosaccharide aqua control cucumber downy mildew field control effectiveness test in Yangling District Dong Wei shop, Shaanxi Province, verified that this medicament is to the control efficiency of cucumber downy mildew, effective dose and to the safety of cucumber.Trial crops is a cucumber, controlling object cucumber downy mildew ([pseudoperonospra cubersis (Berk.et curt) Rostov]).Test medicine and dosage are 1000 times, 1500 times, 2000 times in 0.5% isothiazolinone amino-oligosaccharide aqua, 1000 times in 0.5% isothiazolinone aqua, and 500 times in 0.5% amino-oligosaccharide aqua adopts conventional spray-on process.
Investigation state of an illness radix before the dispenser is respectively at carrying out the preventive effect investigation behind 7 days and the 3rd medicine behind 7 days, the 2nd time medicine behind the 1st medicine in 7 days.Every sub-district is got 5 points at random, whole blades of every some investigation 2 strains.
The blade grade scale:
0 grade: no scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6%～10% of whole leaf area;
5 grades: lesion area accounts for 11%～25% of whole leaf area;
7 grades: lesion area accounts for 26%～50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area;
The drug effect computational methods:
In the formula: CK
0, CK
1Be respectively the forward and backward disease index of blank district dispenser;
PT
0, PT
1Be respectively the forward and backward disease index of chemicals treatment district dispenser.
Table 7 0.5% isothiazolinone amino-oligosaccharide aqua control cucumber downy mildew result of the test
Table 7 for behind the medicine the 7th day the time each sub-district preventive effect carry out the biostatistics analysis, analysis result shows: handle differences and reach extremely outstanding level, district's group difference is not remarkable.Significance of difference comparative result shows: consistent with the significance of difference on 0.01 level on 0.05 level, 1000 times in 0.5% isothiazolinone amino-oligosaccharide aqua, 1500 times, the difference of comparing in twos between 2000 times of 3 processing all reaches utmost point significance level, its 1000 times, respectively handle differences with other for 1500 times and all reach utmost point significance level, 2000 times in 0.5% isothiazolinone amino-oligosaccharide aqua is all remarkable with 1000 times 2 processing of contrast medicament 0.5% isothiazolinone aqua differences, and both all reach utmost point significance level with 500 times of processing of 0.5% amino-oligosaccharide aqua differences.
Field trial shows: 0.5% isothiazolinone amino-oligosaccharide aqua has remarkable prevention effect to cucumber downy mildew, its 1000 times, 1500 times control efficiency obviously is better than contrasting the control efficiency of 1000 times in medicament 0.5% isothiazolinone aqua, 500 times of processing of 0.5% amino-oligosaccharide aqua, after result of the test shows that isothiazolinone and amino-oligosaccharide are mixed, control efficiency and drug cost have obviously been improved, to cucumber growth safety to cucumber downy mildew.
Embodiment 4 control tomato late blight field trials
In the green house of Yangling District Dong Wei shop, Shaanxi Province, carried out 1% isothiazolinone amino-oligosaccharide aqua control tomato late blight field control effectiveness test, verified that this medicament is to the control efficiency of tomato late blight, effective dose and to the safety of tomato.Trial crops is a tomato, controlling object tomato late blight (Phytophthora infestansde Bary).Test medicine and dosage are 1500 times in 1% isothiazolinone amino-oligosaccharide aqua, 2000 times 2500 times, 750 times in 0.5% isothiazolinone aqua, and 400 times in 0.5% amino-oligosaccharide aqua adopts the complete stool spray-on process.
Investigation state of an illness radix before the dispenser is respectively at carrying out the preventive effect investigation behind 7 days and the 3rd medicine behind 7 days, the 2nd time medicine behind the 1st medicine in 7 days.Every sub-district is got 5 points at random, and every some investigation 2 strains, every strain divide the upper, middle and lower investigation 10 leaves, and the percentage that accounts for the whole blade area with lesion area on each blade comes classification.
The blade grade scale:
0 grade: no scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6%～10% of whole leaf area;
5 grades: lesion area accounts for 11%～20% of whole leaf area;
7 grades: lesion area accounts for 21%～50% of whole leaf area;
9 grades: lesion area accounts for more than 165% of whole leaf area;
The drug effect computational methods:
In the formula: CK
0, CK
1Be respectively the forward and backward disease index of blank district dispenser;
PT
0, PT
1Be respectively the forward and backward disease index of chemicals treatment district dispenser.
Table 8 1% isothiazolinone amino-oligosaccharide aqua control tomato late blight result of the test
Table 8 for behind the medicine the 7th day the time each sub-district preventive effect carry out the biostatistics analysis, analysis result shows: handle differences and reach extremely outstanding level, district's group difference is not remarkable.Significance of difference comparative result shows: consistent with the significance of difference on 0.01 level on 0.05 level, 1% isothiazolinone amino-oligosaccharide aqua is respectively handled differences with other for 1500 times, 2000 times and is all reached utmost point significance level, its 2500 times are not remarkable with 750 times 2 processing of contrast medicament 0.5% isothiazolinone aqua differences, and both all reach utmost point significance level with 400 times of processing of 0.5% amino-oligosaccharide aqua differences.
Field trial shows: 1% isothiazolinone amino-oligosaccharide aqua has remarkable prevention effect to tomato late blight, its 1500 times, 2000 times control efficiency obviously is better than contrasting the control efficiency of 750 times in medicament 0.5% isothiazolinone aqua, 400 times of processing of 0.5% amino-oligosaccharide aqua, after result of the test shows that isothiazolinone and amino-oligosaccharide are mixed, obviously improved the control efficiency of tomato late blight and reduced drug cost, to tomato growth safety.
In sum, composition of the present invention is to adopt two kinds of active component compound schemes, bactericidal effect is good, drug cost is low, and its active and bactericidal effect is not the simple superposition of each composition activity, but significant synergistic effect is arranged, quick-acting height, lasting effect are long, slow down the generation of resistance, good to crop safety, meet the security requirement of pesticidal preparations.
Claims (6)
1, a kind of bactericidal composition of isothiazolone-containing is characterized in that comprising:
A) the first active component isothiazolinone;
B) the second active component amino-oligosaccharide;
The weight ratio of first active component and second active component is 0.01～140:1.
2, the bactericidal composition of a kind of isothiazolone-containing according to claim 1 is characterized in that: the weight ratio of first active component and second active component is 0.02～60:1.
3, the bactericidal composition of a kind of isothiazolone-containing according to claim 2 is characterized in that: the weight ratio of first active component and second active component is 0.04～30:1.
4, the bactericidal composition of a kind of isothiazolinone according to claim 3 is characterized in that: the weight ratio of first active component and second active component is 0.05～5:1.
5, according to the bactericidal composition of one of any described isothiazolone-containing of claim 1 to 4, it is characterized in that: described composition is the aqua formulation.
6, the application of one of any described isothiazolone-containing bactericidal composition of claim 1 to 4 in control fruit tree, cereal class, melon and fruit class, greengrocery crop pest.
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| CN102771488A (en) * | 2012-07-31 | 2012-11-14 | 陕西上格之路生物科学有限公司 | Bactericidal composition containing isothiazolone |
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| CN100579970C (en) * | 2007-05-17 | 2010-01-13 | 湖北达雅化工技术发展有限公司 | Method for preparing bactericide of iso-thiazolinone |
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