CN101530050A

CN101530050A - Thin-skin walnut indoor micro-branch engrafting method

Info

Publication number: CN101530050A
Application number: CN200910113253A
Authority: CN
Inventors: 李冠; 苗玉青
Original assignee: Xinjiang University
Current assignee: Xinjiang University
Priority date: 2009-03-12
Filing date: 2009-03-12
Publication date: 2009-09-16

Abstract

The invention provides a thin-skin walnut indoor micro-branch engrafting method, belonging to the walnut vegetative propagation technique. The micro-branch engrafting method uses the health tissue culture seedlings of good thin-skin walnut breed (strain) and the health tissue culture seedlings are cut into vaneless stem segments in length of 2.0cm-4.0cm as scions and the scions are engrafted on rootstocks in stem diameter of about 3mm-7mm, and the engrafting cuts are banded using plastic strips and the engrafted seedlings are planted in the nutrient bags and the nutrient bags are capped to keep humidity and the nutrient bags with engrafted seedlings are cultured in a greenhouse of 22-28 DEG C. When the branches to be engrafted grow into 3.0cm-5.0cm, the seedlings are trained and the engrafted seedlings are transplanted into big field after 30 days. The engrafting survival rate can be more than 70%. The engrafting method is free from the limitation of the season condition factor with features of high propagation coefficient, quick seedlings establishment speed, utilization of factory seedlings raising. The engrafting method is suitable for improving the breeds and enlarging the scale of the walnut industry and developing the forestry and fruit industry.

Description

A kind of thin-skin walnut indoor micro-branch engrafting method

Technical field

The present invention relates to fruit tree tissue culture technique and culture technique, be specially the indoor micro-branch graft technology in the vegetative technical field of thin skin walnut.

Background technology

Xinjiang is one of walnut original producton location, and cultivation history is long, and is widely distributed, aboundresources.Xinjiang thin skin walnut shell is thin, easily get benevolence, kernel percent height, benevolence are sweet full, has very high economy, ecology and social value, is good varieties of plant, but be subjected to the restriction of nursery stock quality and quantity, planting scale is less, and supply falls short of demand for product, can not satisfy market demand far away.

At present, the propagation method of thin skin walnut is based on breeding and the traditional grafting of growing directly from seeds.The nursery stock of breeding of growing directly from seeds easily morphs, so that influences the thin skin walnut quality, traditional propagation by grafiting is then more be subjected to improved variety scion quantity and season condition restriction, therefore, traditional propagation method is the obstacle that restriction thin skin walnut improved seeds are promoted always.

Summary of the invention

In order to solve the difficult problem of thin skin walnut tradition breeding, the invention provides a kind of thin-skin walnut indoor micro-branch engrafting method.

Particular content of the present invention, promptly the concrete grammar of indoor micro-branch grafting method is as follows:

(1) cultivation of scion and choose quality better, the excellent thin skin walnut stem section of proterties is carried out tissue culture as explant, minimal medium is DKW, sucrose concentration is 30g/L, agar concentration is 7g/L, pH value 5.8 before the medium sterilization, cultivation temperature is 22 ℃-28 ℃, illumination 14h/d, and choosing healthy and strong tissue cultivating seedling during grafting is scion.

(2) cultivation of stock and choose the excellent walnut seed of resistance, soak after 5-8 days in the flowing water and shone in the sun 1-2 days, after its suture is split, put into the seedbed plantation, water with the spray of 10% potassium permanganate before use in the seedbed, in the planting process, note checking walnut growing state in the seedbed, run into the seed that goes rotten and in time remove, when the long 2.0cm-4.0cm of walnut root, in time be completely cured, the root section is stained with and is continued to be embedded in the seedbed behind the ash and cultivate, and can be used as graft stock when treating the about 3mm-7mm of stock stem rugosity.

(3) the grafting scion and the stock that will obtain through step (1), step (2) carries out cleft grafting indoor.Before the grafting tissue cultivating seedling is cut into the long no base of leaf section of 2.0cm-4.0cm as scion, base portion is whittled into tangent plane length is the wedge shape about 1.1cm.Will be apart from the horizontal partial application in the high 5.0cm of rootstock seedling base portion place, it is standby vertically to cut 1.0cm in incision again, the scion insertion of handling well is ready in many stocks longitudinal incision, note making the cambium alignment of scion and stock, with from bottom to top colligation of plastic strip, notice preventing that interface from seeing light, again grafting is transplanted in the culture bag, and bagging to be to keep 70% humidity, is placed in 22 ℃-28 ℃ the greenhouse to cultivate.

(4) transplant when the grafting sprout sprouts, remove bagging it is fully grown, in time erase, treat to carry out the hardening exercise behind the grafted branch growth 3.0cm-5.0cm if any sprout tillers, the processing of shading when high noon, intensity of illumination was high during the hardening can be carried out field-transplanting with grafting after 30 days.

Patent of the present invention by to the cultivation of scion and selection, stock grow seedlings and choose, the condition of culture of grafting and binding method, grafting etc. studies, set up the comparatively feasible thin skin walnut grafting technical system of a cover, this technology is not limited by the season condition element, have the reproduction coefficient height, become seedling fast, be beneficial to advantage such as factorial seedling growth.Help the development of improved variety, scale and the industry of planting forest or fruit tress of walnut industry.

Description of drawings

Fig. 1 is scion and stock grafting state.

Fig. 2 is the upgrowth situation of grafting after 15 days.

Fig. 3 is a grafting upgrowth situation during the hardening.

Embodiment

Walnut with the local plantation in Xinjiang is the stock seed, with the leatheroid walnut, temperature 185, healthy and strong tissue cultivating seedling such as new 2 walnuts such as thin skin such as grade is that the indoor micro-branch grafting is carried out in scion: the tissue cultivating seedling of getting good stalwartness, be cut into the long no base of leaf section of 2.0cm-4.0cm as scion, after the scion base portion being whittled into the wedge shape of 1.1cm length, adopt the cleft graft grafting to be about on the 3mm-7mm stock to the stem rugosity, and with plastic strip colligation grafting otch from bottom to top, with the grafting seed in nutritious bag, and bagging is to keep 70% humidity, prevent that interface from seeing light, place 22 ℃-28 ℃ greenhouse to cultivate grafting, sprout with scion and remove sprouting for surviving standard.Treat to carry out hardening behind the grafted branch growth 3.0cm-5.0cm, grafting can be carried out field-transplanting after 30 days.Utilize this grafting, graft survival rate can reach 70%.

Claims

1, the little branch grafting method of a kind of indoor thin skin walnut is characterized in that, the cultivation by high-quality thin skin walnut tissue cultivating seedling and the good stock of resistance and choose, grafting, transplanting, obtain micrografting thin skin walnut seedling.

2, according to the described method of claim 1, it is characterized in that choosing quality better, the excellent thin skin walnut stem section of proterties is carried out tissue culture as explant, minimal medium is DKW, sucrose concentration is 30g/L, agar concentration is 7g/L, pH value 5.8 before the medium sterilization, the tissue cultivating seedling cultivation temperature is 22 ℃-28 ℃, chooses healthy and strong tissue cultivating seedling during grafting as scion.

3, method according to claim 1, it is characterized in that choosing the excellent walnut of resistance, soak after 5-8 days in the flowing water and shone in the sun 1-2 days, its suture is split, put into the seedbed plantation, water with 10% potassium permanganate spray before use in the seedbed, in time is completely cured during the long 2.0cm-4.0cm of root, can be used as graft stock when treating stock stem rugosity 3mm-7mm.

4, method according to claim 1, it is characterized in that high-quality thin skin walnut tissue cultivating seedling and the good stock of rugosity 3mm-7mm resistance, carry out cleft grafting indoor, before the grafting tissue cultivating seedling is cut into the long no base of leaf section of 2.0cm-4.0cm as scion, the scion base portion is whittled into tangent plane length is the wedge shape of 1.1cm, then will be apart from the horizontal partial application in the high 5cm of rootstock seedling base portion place, it is standby vertically to cut 1.0cm in incision again, the scion of handling well is inserted in the ready stock longitudinal incision, note making the cambium alignment of scion and stock, with plastic strip colligation interface from bottom to top, prevent that interface from seeing light, grafting is transplanted in the culture bag, and bagging places 22 ℃-28 ℃ greenhouse to cultivate grafting to keep 70% humidity.

5, method according to claim 1 is characterized in that when the grafting sprout sprouts, and removes bagging it is fully grown, and treats to carry out the hardening exercise behind the grafted branch growth 3.0cm-5.0cm, after 30 days grafting is transplanted the land for growing field crops.