CN101520428B - Electrochemical formula sensing method and test piece - Google Patents
Electrochemical formula sensing method and test piece Download PDFInfo
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- CN101520428B CN101520428B CN 200810081128 CN200810081128A CN101520428B CN 101520428 B CN101520428 B CN 101520428B CN 200810081128 CN200810081128 CN 200810081128 CN 200810081128 A CN200810081128 A CN 200810081128A CN 101520428 B CN101520428 B CN 101520428B
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Abstract
The invention provides an electrochemical formula sensing method and a test piece thereof; wherein the electrochemical formula sensing test piece includes an insulating substrate, a reaction groove, acovering part, an electrode assembly and an electrochemical reaction layer; wherein the reaction groove is arranged on the insulating substrate and the covering part is covered on the reaction groove ; the electrochemical reaction layer is connected with one end of the electrode assembly which is arranged in the reaction groove and includes a first electrode and a second electrode, wherein the distance between the first electrode and the second electrode is larger than 100 mu m.
Description
Technical field
The invention relates to an electrochemical formula sensing method and implement the invention of the electrochemical sensing chip of the method, espespecially relevant with a kind of deserted electrochemical formula sensing method and test piece invention.
Background technology
The present invention applies for a patent the continuity of No. the 200510066843.2nd, sequence number before being.The present invention continue the basic framework of this patent＜but changed test block structure, the electrochemical sensing chip (Electrochemical Sensor Strip) that has metal electrode (Metal Electrode) to produce another kind, it is applicable to test a content for the treatment of certain determinand in the fluid measured, such as: the concentration of the materials such as the heavy metal in blood sugar, uric acid, cholesterol concentration and the sewage in the test blood and agricultural chemicals; In other words, be applicable to the detecting device (such as blood-glucose detector) etc. of various electric chemical formula detecting device, biology sensor, fluid biochemical component sensor and various house medical use by the made deserted electrochemical sensing chip of the present invention.
Electrochemical sensing chip (Electrochemical Sensor Strip) has been applied in the detection of various fluids maturely, and its basic framework is to comprise: 1. one accommodating one container that takes up for the treatment of fluid measured; 2. a chemical reagent (Reagent) is used for producing an electrochemical action with this determinand for the treatment of fluid measured, and produces the output signal of an electrical parameter, and the output valve of this electrical parameter is relevant with this determinand for the treatment of fluid measured; 3. a plurality of test electrodes, it comprises electrode (Counter Electrode), working electrode (Working Electrode), reference electrode (Reference Electrode) and detecting electrode (Detecting Electrode).Wherein, when the blood for the treatment of that fluid measured is behaved, and determinand can utilize a glucose oxidase ferment and other compound to be used as chemical reagent when being the blood candy so.
United States Patent (USP) the 5th, 997, No. 817 is an invention of using metal electrode, it is with two same sizes and coating＜Coating all〉one deck precious metal palladium＜Palladium bonding jumper as the metal electrode on the insulated substrate (its be applied to working electrode with on the electrode), but, because of the consumptive material of precious metal palladium larger, and really need to use precious metal palladium material characteristic person only with the contacted part of electro-chemical reaction layer (as long as remaining part can conduct electricity), so whole surface of metal electrode all coating cymbals metal reality be a kind of meaningless waste.
In addition, United States Patent (USP) the 5th, 985, No. 116 then is a deserted example that prints electrode, it need use the electrode by pasty state conductive paste printing moulding, about the one-tenth branch of the pasty state conductive paste that the sensing effect that it has is then used; When with noble metal during as the pasty state conductive paste, it can have better susceptibility and lower chemical interference effect, but but unusual height of cost; Otherwise if the lower conductive carbon film of use cost is made electrode, though it can effectively reduce required cost, but the electrode that obtains causes the error of measurement signal than Gao Eryi because of impedance.
Yet, although various electrochemical sensing chip become now at home medical test strip (for example: the main force test strip of blood sugar, uric acid, cholesterol), detect degree of accuracy and cost of manufacture and but all have again improved space.
See also Fig. 1, it is the making process flow diagram of commonly using electrochemical sensing chip, and its step comprises: 1. an insulated substrate is provided; 2. set up electrode on this insulated substrate; 3. the group structure goes out a reaction groove on this insulated substrate; 4. in this reaction groove, reach this electrode and apply a chemical reagent to form an electro-chemical reaction layer; 5. treat dry this reaction groove of rear enclosed of chemical reagent; 6. wash away an opening in the mode of washing away at the side of insulated substrate.
See also Fig. 2 (A)-(C), wherein electrochemical sensing chip the constitutional diagram before flush operation of Fig. 2 (A) for commonly using, electrochemical sensing chip the constitutional diagram flush operation after of Fig. 2 (B) for commonly using, Fig. 2 (C) is then for to treat that fluid measured enters the synoptic diagram of commonly using electrochemical sensing chip; Wherein this electrochemical sensing chip 1 comprises insulated substrate 11, electrode 12, electro-chemical reaction layer 13, reaction groove 14, overlay 15 and pore 17.Shown in Fig. 2 (A), this reaction groove 14 is a groove that seals all around before not washing away, when a liquid chemical reagent enters (not icon), this chemical reagent just can not flow out outside the reaction groove 14, after the chemical reagent drying, namely generate this electro-chemical reaction layer 13, process this electrochemical sensing chip 1 so that it has the side openings 16 shown in Fig. 2 (B) with a flush operation more at last, wherein determinand A enters reaction groove 14 and then with this electro-chemical reaction layer 13 electro-chemical reaction occurs via this opening 16 that is positioned at electrochemical sensing chip 1 side, and causes a signal.Wherein, when processing electrochemical sensing chip 1 in the mode of washing away, it also can wash out this electro-chemical reaction layer 13 of a part, and wash away in the process at this, this electro-chemical reaction layer 13 may chap 131 because being subject to washing away producing, 131 tops that are positioned at this electrode 12 if chap, then after making this determinand A enter reaction groove 14, it may with before the reaction of the chemical reagent of electro-chemical reaction layer 13 namely directly not contact with this electrode 12 and electro-chemical reaction outside having occured to expect, and then will reduce the degree of accuracy of electrochemical sensing chip 1.
Simultaneously, the electrochemical sensing chip of commonly using may reduce the susceptibility of sensing test piece when interelectrode distance is excessive.But when interelectrode distance is too tight, because electrode distance is excessively near, cause substrate breakage and slight crack between two electrodes, then can cause test piece bad.These have all increased commonly uses the electrochemical sensing chip difficulty of using and the cost of making.
For the cost of manufacture of the metal electrode that reduces deserted electrochemical transducer[sensor test piece with and Effective Raise measure degree of accuracy, increase the facility on using.This case inventor obtains a kind of deserted electrochemical formula sensing method and test piece finally through after repeatedly testing, test and studying.The present invention also can effectively avoid sensing test piece to be subjected in use unnecessary destruction and to improve the detection degree of accuracy except can effectively reducing the test piece cost and simplifying the sensing flow process.
Summary of the invention
The invention provides a kind of electrochemical sensing chip, this electrochemical sensing chip comprises an insulated substrate, a reaction groove, a covering section, an electrode assembly and an electro-chemical reaction layer.Wherein, this reaction groove is positioned on this insulated substrate, and this covering section then covers on this reaction groove.This electro-chemical reaction layer links to each other with this electrode assembly one end.This electrode assembly is positioned at this reaction groove, and comprises one first electrode and one second electrode, and wherein this first electrode and this second electrode are apart greater than 1000 μ m.
According to above-mentioned conception, wherein this insulated substrate system one of them is formed by Polyvinylchloride, polypropylene, polycarbonate, poly terephthalic acid butenylidene ester, polyethylene terephthalate, the inferior benzene of polyoxygenated, acrylonitrilebutadienestryrene resin.
According to above-mentioned conception, wherein this insulated substrate and this reaction groove are one-body molded.
According to above-mentioned conception, wherein this reaction groove comprises a first area and a second area, and this second area is between an edge of this first area and this insulated baseboard.
According to above-mentioned conception, wherein this reaction groove has an opening that communicates with the external world at this edge of this insulated baseboard.
According to above-mentioned conception, wherein the roughness of this second area is lower than the roughness of this first area.
According to above-mentioned conception, wherein the width of this second area is 0.05mm to 4.0mm.
According to above-mentioned conception, wherein this reaction groove has at least two perforations.
According to above-mentioned conception, wherein this electrode assembly is positioned within this perforation.
According to above-mentioned conception, wherein should covering section more comprise a pore.
According to above-mentioned conception, wherein this electrode assembly more comprises a conductive matrices and a metallic film.
According to above-mentioned conception, wherein the material of this conductive matrices system be selected from a metal and contain this metal composition one of them.
According to above-mentioned conception, wherein this metal system is selected from copper, titanium, nickel, gold, platinum, palladium, rhodium, ruthenium, iridium, silver, chromium, iron, aluminium.
According to above-mentioned conception, wherein this first electrode and this second electrode are 1100 μ m at a distance of the best.
According to above-mentioned conception, wherein this first electrode is a working electrode (workingelectrode).
According to above-mentioned conception, wherein this second electrode be a reference electrode (Referenceelectrode) and pair of electrodes (counter electrode) one of them.
According to above-mentioned conception, wherein this electro-chemical reaction layer more comprises a chemical reagent, is in order to a determinand one electro-chemical reaction to occur.
Moreover, this case is to have proposed a kind of electrochemical formula sensing method, it comprises the following step: a reaction groove is provided, one electrode assembly is set in this reaction groove, wherein this electrode assembly comprises at least one the first electrode and one second electrode, and the distance of keeping this first electrode and the second electrode is more than 1000 μ m; Form an electro-chemical reaction layer at this electrode assembly, and cover this reaction groove with an overlay; Provide one to treat that fluid measured reacts groove in this, make this treat that fluid measured one of comprises determinand and one electro-chemical reaction occurs this electro-chemical reaction layer, to cause a signal; And utilize this electrode assembly detection and transmit this signal.
According to above-mentioned conception, this electrochemical formula sensing method more is included in and forms a first area and a second area in this reaction groove, wherein this second area is between an edge of this first area and this reaction groove, and the roughness of this second area is the roughness that is lower than this first area.
According to above-mentioned conception, wherein this electrode is to form in a conductive matrices via coating one metallic film.
According to above-mentioned conception, wherein this metallic film is to utilize spray paint one of them mode of plating, immersion plating, precipitated metal, printing, metal to coat this conductive matrices.
According to above-mentioned conception, wherein this electro-chemical reaction series of strata mat coated and drip a chemical reagent in this reaction groove one of them mode and realize.
According to above-mentioned conception, this electrochemical formula sensing method more is included in this reaction groove and forms and an extraneous opening that communicates in an edge of this insulated substrate.
According to above-mentioned conception, this electrochemical sensing chip more is included in this covering section and forms a pore, in order to regulate the pressure of this reaction groove.
Description of drawings
Fig. 1 is the making process flow diagram of the electrochemical sensing test piece commonly used;
Fig. 2 (A) is the constitutional diagram of electrochemical sensing test piece before carrying out flush operation of commonly using;
Fig. 2 (B) is the constitutional diagram of electrochemical sensing test piece after carrying out flush operation of commonly using;
Fig. 2 (C) enters the synoptic diagram of commonly using electrochemical sensing test piece for determinand;
Fig. 3 is the process flow diagram of electrochemical sensing method for testing of the present invention;
Fig. 4 (A) is the electrochemical sensing test piece explosive view of the preferred embodiment of this case;
Fig. 4 (B) is the constitutional diagram of the electrochemical sensing test piece of the preferred embodiment of this case;
Fig. 4 (C) is the back view of the electrochemical sensing test piece of the preferred embodiment of this case;
And Fig. 5 (A)-(B), detect the synoptic diagram of a determinand for the electrochemical sensing test piece of a preferred embodiment of using this case.
The primary clustering symbol description
A: determinand S1,1: electrochemical sensing chip
11: insulated substrate 12: electrode
13: electro-chemical reaction layer 131: defective
14: reaction groove 15: overlay
16: opening 17: pore
2: insulated substrate 21: the reaction groove
211: perforation electrode 212: to electrode
2121,2122: metallic film 2123: conductive matrices
213: working electrode
2131,2132: metallic film 2133: conductive matrices
214: first area 215: second area
22: electro-chemical reaction layer 23: covering section
231: pore 232: view window
24: reaction groove 25: edge
Embodiment
The structure of the electrochemical sensing chip of this case and method for sensing can and be fully understood by following embodiment explanation, and so that have the knack of that the personage of this skill can finish according to this, the enforcement kenel of right this case is not restricted in the following example.
See also Fig. 3, be the process flow diagram of electrochemical formula sensing method of the present invention; Its step comprises: 1. a reaction groove is provided; 2. an electrode assembly is set in this reaction groove; 3. coating one liquid or gluey chemical reagent in the reaction groove form one and the contacted electro-chemical reaction layer of this electrode assembly after drying; 4. cover this reaction groove with an overlay; 5. provide one to treat that side liquid reacts groove in this, make this treat that fluid measured one of comprises determinand and one electro-chemical reaction occurs this electro-chemical reaction layer, to cause a signal; And 6. utilize this electrode assembly to detect and transmit this signal.Wherein, this reaction groove have one with the external world between the opening that communicates.This reaction groove position and utilizes plastic injection with this insulated substrate and one-body molded on an insulated substrate.Simultaneously, in this reaction groove, further form a first area and a second area, and this second area is between an edge of this first area and this reaction groove, and the roughness of this second area is the roughness that is lower than this first area.This electrode assembly then comprises at least one the first electrode and one second electrode, and the distance of keeping this first electrode and this second electrode is more than 1000 μ m.And this electrode is to form in a conductive matrices via coating one metallic film.Common conductive matrices comprises: metal or other the alloys such as copper, titanium, nickel, gold, platinum, palladium, rhodium, ruthenium, iridium, silver, chromium, iron or aluminium, also or the material of other tool electric conductivity such as carbon form.The material of metallic film then is to be selected from copper, titanium, nickel, gold, platinum, palladium, rhodium, ruthenium, iridium etc.This metallic film then is to utilize the modes such as plating, immersion plating, precipitated metal, printing, metal spray paint to coat on this conductive matrices.In addition, the normal insulated substrate that uses is by Polyvinylchloride, polypropylene, polycarbonate, poly terephthalic acid butenylidene ester, polyethylene terephthalate, the inferior benzene of polyoxygenated or the acrylonitrilebutadienestryrene resin is formed.Enter this reaction groove and get and the mode such as this electro-chemical reaction layer mat is coated, distribution or drippage applies a chemical reagent.Should then form at least one pore in the covering section simultaneously, this pore is the convection holes of a capillary flow path, so that the pressure in the conditioned reaction groove, and and then adjust this and treat the distribution of fluid measured in this reaction groove.Because the present invention sees through the assembly that will have particular geometries to be made up, so successfully exempted the step of washing away of commonly using, also therefore can avoid the chemical reaction layer to suffer the destruction of the stress of non-essential fully.Simultaneously, repeatedly test through the inventor, optimum distance is 1100 μ m between electrode.This distance makes the sensing effect of electro-chemical reaction that better susceptibility be arranged, and also effectively improves when interelectrode distance and crosses when near, causes that insulated substrate breaks and slight crack between two electrodes, the bad problem of the test piece that produces.
See also Fig. 4 (A)-(C), Fig. 4 (A) is the electrochemical sensing chip explosive view of a preferred embodiment of this case; Fig. 4 (B) is the constitutional diagram of this electrochemical sensing chip; Fig. 4 (C) then is the back view of this electrochemical sensing chip.Shown in Fig. 4 (A)-(C), the electric chemical formula induction test piece S1 of this case comprises: one has insulated substrate 2, the electro-chemical reaction layer 22 in this reaction groove 21, a covering section 23, an opening 24 and a uneven edge 25 of a reaction groove 21, wherein should covering section 23 have a pore 231 and a view window 232.And after reaction groove 21 was covered in covering section 23, this reaction groove 21 was to have opening 24 at the edge of this insulated substrate 2; In addition, be to have a first area 214 and a second area 215 at reaction groove 21.Generally speaking, the width of second area 215 is 0.05mm to 4.0mm, and wherein with 0.1mm to 2.0mm for better.In addition, the roughness of this first area 214 is the roughness greater than second area 215, therefore, after aqueous chemical reagent (not icon) is coated the first area 214 and second area 215 of reaction groove 21, the adhesion that produces between this aqueous chemical reagent and this first area 214 will be greater than the adhesion that produces between this aqueous chemical reagent and this second area 215, this will be so that difficult the spilling in this first area 214 of this aqueous chemical reagent flows to opening 24 places, utilize two different zones of surfaceness to make chemical liquid produce different surface tension and unlikely outflow has outside the groove of opening, this is one of characteristic of this case, be different from the conventional practice, must make first the groove of a sealing opening, splash into liquid chemical reagent and recycle after drying the feed opening that stamped one is treated fluid measured, the punching course that causes easily causes dry chemical reaction layer be full of cracks and the problem of impact measurement degree of accuracy.In addition, meriting attention is that 215 of this first area 214 and this second areas can have a difference in height.Moreover, because there is uneven edge 25, thus the opening 24 whole chances that sealed fully by determinand will reduce and be beneficial to determinand enter the reaction groove 21.
In addition, be to have two perforations 211 in reaction groove 21 bottoms, and can run through these perforations 211 to electrode (Counter Electrode) 212 (the first electrode) and working electrode (WorkingElectrode) 213 (the second electrode), wherein the end facet of electrode 212 and working electrode 213 respectively had metallic film 2121,2122,2131 and 2132, and comprise conductive matrices 2123 and 2133 (seeing also Fig. 4 (A) and the Fig. 4 (C)); Wherein metallic film 2121 contacts with this electro-chemical reaction layer 22 with 2131, in order to detect the electro-chemical reaction that comes from the 22 interior generation of electro-chemical reaction layer.Because the invention provides the electrochemical sensing chip S1 with uneven edge 25 and opening 24, so, in manufacturing process, need not commonly use wash away step (in order to produce one can for test substance enter the reaction groove entrance), therefore use the integrality that the present invention can guarantee electro-chemical reaction layer 22, and and then the degree of accuracy of promoting electro-chemical reaction.In addition, the position of the reaction groove 21 of this case, covering section 23 and opening 24 and shape should be not limited to the enforcement kenel of present embodiment.
In addition, mention such as prior art, between the sensing electrode of known electrochemical sensing chip (such as present embodiment to electrode 212 and working electrode 213), distance can not be too far away, in order to avoid reduce the susceptibility of sensing test piece.If but distance is too near, then can cause insulated substrate to break and the generation slight crack.In the present embodiment, to electrode 212 and working electrode 213 distances 1100 μ m, can effectively reduce the impact of electrode pair test block structure, therefore the fraction defective that produces because breaking also can reduce, thereby solves for a long time not high enough problem of yield of the art.
Further please refer to Fig. 5 (A)-(B), it is to utilize the electrochemical sensing chip of the preferred embodiment of this case to detect the synoptic diagram of an a certain determinand for the treatment of fluid measured (for example detect blood sugar, uric acid level in the blood, or detect mercury content in the sewage etc.).When a test substance A enters reaction groove 21 by opening 24, this electro-chemical reaction occurs with electro-chemical reaction layer 22 interior contained chemical substance in this test substance A immediately, and pore 231 within can getting rid of reaction groove 21 portion gas and make treat that fluid measured A can distribute smoothly, it also can be used to lead off the gas that produces in this electro-chemical reaction, advance to guarantee to react under the state that maintains air pressure balance inside and outside the groove 21 and make these electro-chemical reactions in this reaction groove 21 not be subject to non-essential impact.Observation window 232 comprises a hyaline membrane, whether be covered with reaction groove 21 fully in order to when test substance A enters reaction groove 22, observe test substance A, if can in time replenish when test substance fails to be covered with, in order to avoid produce abnormal electro-chemical reaction because test substance A is not enough.Design a uneven edge 25 in opening 24 places, this opening 24 can be sealed fully and make test substance A can't flow in the groove when adding test substance A to avoid, when for example doing at home blood glucose value test, prick a pin at finger, extrude a droplet of blood on finger, when tightly leaning on into opening 24, finger opening 24 may be sealed fully, blood (being test substance A) can't be flowed in recessed 21 grooves of reaction smoothly, the user should be noted and can only lean on gently into opening 24 that uneven edge 25 designs of opening 24 can be avoided opening 24 to be sealed fully and cause the shortcoming that test substance A can't flow into reaction groove 21; In addition, because pore 231 has been regulated in the reaction groove 21 air pressure, but therefore also so that object under test A mat capillarity and enter reaction groove 21.In addition, where necessary, can replace this to electrode 212 by a reference electrode, and other electrode can be set with the usefulness as detecting electrode more.At present often be used to make electrode or the material of metallic film comprises: metal and the materials such as alloy or carbon thereof such as copper, titanium, nickel, gold, platinum, palladium, rhodium, ruthenium, silver, iron or aluminium; And wherein take brass, oxygen-free copper, bronze, phosphor-copper, foreign copper-nickel alloy, beryllium copper as present more common material.Again, the main benefit of doing to design with metallic film collocation electrode is effectively Cost reduction; Because the part that really need to have high response and stability only is the end points of electrode assembly, so, only utilize the noble metal material in this part, and use other metal or carbon-point can effectively reduce the cost of electrode assembly at other position.In addition, the plastics shooting method is one-body molded to be got the insulated substrate of present embodiment in order to utilize with the reaction groove, and this can exempt the cost of commonly using test piece required cost when coinciding with layer structure.
In sum, the present invention sees through special geometry designs has been exempted the step of washing away of commonly using, and it can effectively be avoided the sensing test piece inner structure to be subjected to unnecessary destruction in manufacturing process and cause and detect the shortcoming that degree of accuracy reduces; Therefore what is more, because the present invention washes away step because exempting, no matter be in the making complexity of sensing test piece or on cost, all will significantly reduce.Further because interelectrode distance suitable configuration, more reduce again the fraction defective that sensing test piece produces because of substrate breakage between electrode.So, can be executed that the craftsman thinks and be to modify right neither taking off such as Protector that attached claim is wanted as all by knowing usually that at this field tool the knowledgeable appoints even if this case has been described in detail by the above embodiments.
Claims (15)
1. an electrochemical sensing chip is characterized in that, comprises:
One insulated substrate;
One reaction groove is positioned on this insulated substrate;
One covering section covers on this reaction groove;
One electrode assembly is positioned at this reaction groove, comprises one first electrode and one second electrode;
Two perforations, be formed in this reaction groove, this two perforation state that is set in distance, has a spacing distance between this two perforation and consist of, and this first electrode of this electrode assembly and this second electrode are arranged at respectively in this two perforation, and wherein this first electrode and this second electrode are apart greater than 1000 μ m;
One electro-chemical reaction layer links to each other with this electrode assembly one end.
2. electrochemical sensing chip as claimed in claim 1, it is characterized in that, wherein this insulated substrate system one of them is formed by Polyvinylchloride, polypropylene, polycarbonate, poly terephthalic acid butenylidene ester, polyethylene terephthalate, the inferior benzene of polyoxygenated, acrylonitrilebutadienestryrene resin.
3. electrochemical sensing chip as claimed in claim 1 is characterized in that, wherein this insulated substrate and this reaction groove are one-body molded.
4. electrochemical sensing chip as claimed in claim 1 is characterized in that, wherein:
This reaction groove comprises a first area and a second area, and this second area is between an edge of this first area and this insulated substrate;
This reaction groove has an opening that communicates with the external world at this edge of this insulated substrate;
The roughness of this second area is lower than the roughness of this first area;
And the width of this second area is 0.05mm to 4.0mm.
5. electrochemical sensing chip as claimed in claim 1 is characterized in that, wherein this reaction groove has two perforations, and this electrode assembly is positioned within this perforation.
6. electrochemical sensing chip as claimed in claim 1 is characterized in that, wherein should covering section more comprise a pore.
7. electrochemical sensing chip as claimed in claim 1 is characterized in that, wherein this electrode assembly more comprises a conductive matrices and a metallic film, wherein:
The material of this conductive matrices system be selected from a metal and contain this metal composition one of them;
And this metal system is selected from copper, titanium, nickel, gold, platinum, palladium, rhodium, ruthenium, iridium, silver, chromium, iron, aluminium.
8. electrochemical sensing chip as claimed in claim 1 is characterized in that, wherein this first electrode and this second electrode are 1100 μ m at a distance of the best.
9. electrochemical sensing chip as claimed in claim 1 is characterized in that, wherein this first electrode be pair of electrodes (counter electrode) and a reference electrode (Reference electrode) one of them;
Wherein this second electrode is a working electrode (working electrode).
10. electrochemical sensing chip as claimed in claim 1 is characterized in that, wherein this electro-chemical reaction layer more comprises a chemical reagent.
11. an electrochemical formula sensing method is characterized in that, comprises the following step:
One reaction groove is provided;
One electrode assembly is set in this reaction groove, this electrode assembly comprises at least one the first electrode and one second electrode;
Two perforations are set in this reaction groove, this two perforation state that is set in distance, has a spacing distance between this two perforation and consist of, and this first electrode and this second electrode of this electrode assembly are arranged at respectively in this two perforation, and the distance of keeping this first electrode and the second electrode is more than 1000 μ m;
Form an electro-chemical reaction layer at this electrode assembly;
Cover this reaction groove with an overlay;
Provide one to treat that fluid measured reacts groove in this, make this treat that fluid measured one of comprises determinand and one electro-chemical reaction occurs this electro-chemical reaction layer, to cause a signal;
And utilize this electrode assembly detection and transmit this signal.
12. electrochemical formula sensing method as claimed in claim 11, it is characterized in that, more be included in and form a first area and a second area in this reaction groove, wherein this second area is between an edge of this first area and this reaction groove, and the roughness of this second area is the roughness that is lower than this first area.
13. electrochemical formula sensing method as claimed in claim 11, it is characterized in that, wherein this electrode is to form in a conductive matrices via coating one metallic film, and this metallic film is to utilize spray paint one of them mode of plating, immersion plating, precipitated metal, printing, metal to coat this conductive matrices.
14. electrochemical formula sensing method as claimed in claim 11 is characterized in that, wherein this electro-chemical reaction series of strata mat coated and drip a chemical reagent in this reaction groove one of them mode and realize.
15. electrochemical formula sensing method as claimed in claim 11 is characterized in that, more comprises:
Form and an extraneous opening that communicates in an edge of this insulated substrate at this reaction groove;
And/or form a pore in this covering section, in order to regulate the pressure of this reaction groove.
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| TWI439689B (en) | 2010-09-23 | 2014-06-01 | Bionime Corp | Electrochemical test specimen |
| CN102445471B (en) * | 2010-10-09 | 2014-04-09 | 华广生技股份有限公司 | Electrochemical sensing specimen |
| JP5661424B2 (en) * | 2010-10-29 | 2015-01-28 | アークレイ株式会社 | Electrochemical sensor |
| KR20120049723A (en) | 2010-11-09 | 2012-05-17 | 쓰리엠 이노베이티브 프로퍼티즈 캄파니 | Electrochemical biosensor electrode strip and preparing method thereof |
| CN105092662B (en) * | 2014-05-20 | 2019-01-22 | 光宏精密股份有限公司 | Electrochemical sensing test piece and its manufacturing method |
| CN105277597B (en) * | 2014-07-17 | 2017-09-15 | 光宏精密股份有限公司 | Electrochemical sensing test piece electric-conductor method to set up |
| CN107991354B (en) * | 2016-10-26 | 2020-02-14 | 英属维京群岛商艾格生科技股份有限公司 | Substrate structure of biological sensing test piece and manufacturing method of biological sensing test piece |
| US11506627B2 (en) * | 2019-08-02 | 2022-11-22 | Bionime Corporation | Micro biosensor and measuring method thereof |
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| CN1614403A (en) * | 2003-11-07 | 2005-05-11 | 华广生技股份有限公司 | Structure and producing process for electrochemcial sensoring testing block |
| CN1886652A (en) * | 2003-12-04 | 2006-12-27 | 松下电器产业株式会社 | Hematocrit(hct) measuring method, sensor used for same, and measuring instrument |
| TW200636233A (en) * | 2005-04-07 | 2006-10-16 | Bionime Corp | Structure and manufacturing process of electrochemical sensor strip |
| CN1854728A (en) * | 2005-04-20 | 2006-11-01 | 华广生技股份有限公司 | Electrochemical sensing chip and its production |
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