CN101513194B - Compound desinsection primary agent and pesticide consisting of verticillium lecanii and nimbin - Google Patents
Compound desinsection primary agent and pesticide consisting of verticillium lecanii and nimbin Download PDFInfo
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- CN101513194B CN101513194B CN2009100377505A CN200910037750A CN101513194B CN 101513194 B CN101513194 B CN 101513194B CN 2009100377505 A CN2009100377505 A CN 2009100377505A CN 200910037750 A CN200910037750 A CN 200910037750A CN 101513194 B CN101513194 B CN 101513194B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses a compound desinsection primary agent and pesticide consisting of verticillium lecanii and nimbin. The primary agent is a mixture of the verticillium lecanii and the nimbin, wherein, the mass ratio between the verticillium lecanii and the nimbin is 100:0.05-0.30; while the pesticide consists of a primary agent and a secondary agent; according to mass percent, the primary agent accounts for 20-60%, and the secondary agent 40-80%. The compound pesticide of the invention can be prepared into wettable powder, fungal spore suspending agent and medicinal oil; in addition, the compound pesticide of the invention has better effect in preventing and controlling the pests than the preventing effect when one medical preparation alone such as the nimbin is used and can effectively reduce the use of the nimbin in practical production. Through long-term infection biological study and interior biological assay, the verticillium lecanii has strong infection desinsection effect on a plurality of pests, the sucking pests in particular. The compound pesticide consisting of the verticillium lecanii and the nimbin of the invention has good effect in preventing and controlling pests such as white fly, plant louse, diamond back moth and the like.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to a kind of microorganism insecticide, the Verticillium lecanii and the composite desinsection host and the insecticide of nimbin of particularly a kind of environmental protection, pollution-free, noresidue.
Background technology
Verticillium lecanii (Verticillium lecanii (Zimmermann) Viegas) is a kind of insect pathogenic fungus, as a kind of living microorganism agricultural chemicals, feature with the brand-new mechanism of action that is different from existing chemical insecticide, environmentally safe, noresidue, can be used as the alternative control medicine of some insect that bacillus thuringiensis,Bt (Bt) is developed immunity to drugs of control, the development and use Verticillium lecanii has important economic implications.Verticillium lecanii distributes extensively, Bemisia tabaci, Trialeurodes vaporariorum Westwood, aphid, thrips etc. is had good control efficiency, has wide development, application prospect.
Nimbin (azadirachtin) is acknowledged as the effect that insect is had food refusal, stomach toxicity, tags and suppress to grow, its mechanism of action mainly is the internal secretion of upsetting insect, action target is cerebral nerve secretory cell, corpora cardiaca, prothoracic gland etc., influence promotes the synthetic of prothoracic gland hormone and discharges, lower prothoracic gland and the induction of prothoracic gland hormone is caused the 20-hydroxyecdysone is synthetic, the deficiency of secretion, cause the insectean metamorphosis impaired development.The nimbin preparation shows different physiologically actives to kind of insect surplus 400.Have advantages such as higher mammal low toxicity, low-residual, insect are difficult for developing immunity to drugs.
But the resource-constrained of nimbin, the supply of nimbin preparation is far below the demand in producing.Employing agriculture chemical compounding technology or mixing application technique can reduce the usage amount in the production reality, and it is to improve the quick-acting of microorganism insecticide and an important method of minimizing residue of pesticide that the agricultural chemicals of employing sub-doses mixes with biopesticide.Therefore, the Compositional type insecticide of exploitation nimbin is significant.Especially utilize the composite living body biological agricultural chemicals of insect pathogenic fungus, both can reduce the usage amount of nimbin in the production reality, can realize environmentally safe, noresidue again, have the brand-new mechanism of action that is different from existing chemical insecticide as nimbin.Still find no at present the correlation technique report of the compounded pesticides of relevant Verticillium lecanii and nimbin.
Summary of the invention
Purpose of the present invention mainly is in order to overcome the deficiencies in the prior art, and the Verticillium lecanii and the composite desinsection host of nimbin of a kind of environmental protection, pollution-free, noresidue is provided.
Another object of the present invention provides the insecticide that is prepared by described desinsection host.
Purpose of the present invention is achieved through the following technical solutions:
Provide a kind of Verticillium lecanii and nimbin composite desinsection host, described host is the mixture of Verticillium lecanii and nimbin, and the mass ratio of described Verticillium lecanii and nimbin is 100: 0.05～0.30.
In order to realize the present invention better, the mass ratio of described Verticillium lecanii and nimbin is preferably 100: 0.08～and 0.2.
Described Verticillium lecanii Verticillium lecanii (Zimmermann) Viegas bacterial strain VL-N6, belong to hyphomycetales, Verticillium, be located away from the Bemisia tabaci polypide of being infected in the area, Guangzhou, bacterial strain preserving number: CCTCC No:M-208086, on June 10th, 2008 is in China's typical culture collection center preservation, is to the existing concrete description of Verticillium lecanii in 200810029163.7 the patent application at application number.Described Verticillium lecanii bacteria colony white is to cream-colored, and is thin cotton-shaped, and the back side is colourless to deep mixed yellow.Conidiophore is undeveloped, like nutrient hypha, Dan Sheng is arranged on it, to the bottle stalk of giving birth to or 3～4 verticillate, and the bottle stalk is very thin, and size is because of bacterial strain and cell age is different differs greatly 8.5-40 * 0.8-2.2 μ m; Conidium is oval to cylindrical, two terminal circle, and Dan Sheng is gathered into head at bottle stalk top, and size makes a variation bigger because of bacterial strain reaches, 2.3-10 * 1-2.6 μ m.
As a kind of preferred, described Verticillium lecanii is Verticillium lecanii bacterium powder or Verticillium lecanii spore suspension.
What insecticide of the present invention played insecticidal action is described host, being equipped with the effect that assistant agent obtains conveniently to use according to the existing routine techniques in this area gets final product, as preferred scheme, the present invention provides a kind of insecticide that is prepared by described desinsection host simultaneously, described insecticide is made up of host and assistant agent, host is 20～60% by mass percentage, and assistant agent is 40～80%.
Stabilizing agent, auxiliary agent and filler are respectively by mass percentage that stabilizer comprises compounded pesticides 23～30%, auxiliary agent account for compounded pesticides 7～22%, filler accounts for compounded pesticides 10～35%.
Described stabilizing agent is preferably phosphate buffer or the paraffin oil of glycerine, 0.21M; Described auxiliary agent is preferably one or more mixtures among Tween-80, methyl green, sodium lignin sulfonate, the wetting agent LX; Described filler is kaolin, diatomite or potter's clay.
The compounded pesticides of described Verticillium lecanii and nimbin can be processed into formulations such as wetting powder, suspending agent or finish.
The present invention compared with prior art has following advantage and beneficial effect:
(1) the bacterial strain uses therefor of the present invention Bemisia tabaci polypide of being infected from the area, Guangzhou, Guangdong Province, China separates and obtains, and is China domestic bacterial strain, is not from external introduction, can adapt to the natural environment of this locality.
(2) adopt the agricultural chemicals and the biopesticide of sub-doses to be mixed, determine to obtain the mixed ratio of good insecticidal effect, reduced the consumption and the persticide residue of nimbin, adapted to the requirement that organic food is produced, environmentally safe, noresidue help delaying pest resistance to insecticide and produce.
(3) control efficiency of compounded pesticides of the present invention is better than only using a kind of control efficiency of medicament.Through long-term biological study and the indoor bioassay of infecting, Verticillium lecanii has the very strong insecticidal effect that infects to various pests especially sucking pest.Compounded pesticides of the present invention has good control efficiency to insects such as aleyrodid, aphid, diamond-back moths.
Embodiment
What insecticide of the present invention played insecticidal action is described host, being equipped with the effect that assistant agent obtains conveniently to use according to the existing routine techniques in this area gets final product, the present invention is described in further detail below in conjunction with preferred embodiment, but therefore do not limit scope of the present invention.Stabilizing agent, auxiliary agent and filler can use with reference to this area is conventional, give unnecessary details no longer one by one among the embodiment, but therefore the present invention are not defined among the following embodiment.
Described Verticillium lecanii Verticillium lecanii (Zimmermann) Viegas belongs to hyphomycetales, Verticillium is located away from the Bemisia tabaci polypide of being infected in the area, Guangzhou the bacterial strain preserving number: specifically addressed state Verticillium lecanii.
Percentage is mass percent described in following examples, described verticillium lecanii strain is the VL-N6 bacterial strain, bacterial strain preserving number: CCTCC No:M-208086, on June 10th, 2008 is in China's typical culture collection center preservation, and specifically describing and being found in application number is in 200810029163.7 the patent application.
Embodiment 1:20% Verticillium lecanii nimbin suspending agent (dilution is 2000 times during use)
A partly is 40% Verticillium lecanii conidial suspension: 40% Verticillium lecanii conidial powder is dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M, transfers pH7.0, in 4～8 ℃ of preservations.
B partly is a wetting powder: nimbin (commercial) 0.12%, and Tween-80 20%, sodium lignin sulfonate 6%, wetting agent LX 4% adds potter's clay and mends to 100%.
A part and B part respectively account for 50%, and the direct mixing of two parts is promptly obtained 20% Verticillium lecanii nimbin suspending agent, Verticillium lecanii: the nimbin mass ratio is 100: 0.30.The virulence test result is as shown in table 1.
Embodiment 2:50% Verticillium lecanii nimbin finish (dilution is 2000 times during use)
Verticillium lecanii bacterium powder (10
10Individual/g) 50%, nimbin 0.1% (commercial), Tween-80 5%, paraffin oil 23%, methyl green 2% adds diatomite and mends to 100%, makes 50% Verticillium lecanii nimbin finish.Wherein, Verticillium lecanii bacterium powder: the nimbin mass ratio is 100: 0.2.The virulence test result is as shown in table 1.
Embodiment 3:60% Verticillium lecanii nimbin finish (dilution is 2000 times during use)
Verticillium lecanii bacterium powder (10
10Individual/g) 60%, nimbin 0.05% (commercial), methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin is mended to 100%, makes 60% Verticillium lecanii nimbin finish.Wherein, Verticillium lecanii bacterium powder: the mass ratio of nimbin is 100: 0.08.The virulence test result is as shown in table 1.
Embodiment 4:60% Verticillium lecanii nimbin wetting powder (dilution is 2000 times during use)
Verticillium lecanii bacterium powder (10
10Individual/g) 60%, nimbin 0.09% (commercial), methyl green 2%, Tween-80 10%, sodium lignin sulfonate 6%, wetting agent LX 4%, diatomite is mended to 100%, makes 50% Verticillium lecanii nimbin wetting powder.Wherein, Verticillium lecanii bacterium powder: the mass ratio of nimbin is 100: 0.15.The virulence test result is as shown in table 1.
Embodiment 5:50% Verticillium lecanii nimbin finish (dilution is 2000 times during use)
Verticillium lecanii bacterium powder (10
10Individual/g) 50%, nimbin 0.025% (commercial), methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin is mended to 100%, makes 60% Verticillium lecanii nimbin finish.Wherein, Verticillium lecanii bacterium powder: the mass ratio of nimbin is 100: 0.05.The virulence test result is as shown in table 1.
Embodiment 6: mixture is to the Toxicity Determination of Bemisia tabaci (Bemisia tabaci)
During Toxicity Determination, (Cucumis sativus L.) makes test plant with cucumber, potted plant seedling is put into dependent insect cage, and access Bemisia tabaci adults, treat after it was laid eggs one day it all to be driven out of, keep 26 ± 1 ℃ temperature and 14: 10 illumination in the cage, treat that Bemisia tabaci on the cucumber leaves grows to 2 ages during nymph, adopt infusion process to carry out the virulence test, 4 repetitions, 10 days effect behind the investigation medicine, the calculation correction lethality, ask the regression equation of drug concentration logarithm-lethality probability value, and carry out the F check, obtain LC then
50, calculate relative virus force index (TI) with reference to the abundant method of Sun Yun, its result of the test is as shown in table 1.
Table 1 Verticillium lecanii nimbin is to the Toxicity Determination of Bemisia tabaci
(10
4Spore/ml, μ g/ml) (behind the medicine 10 days)
| Handle | Virulence regression equation | R 2 | LC 50And 95% confidence interval | TI |
| Verticillium lecanii | y＝0.3744x+3.3240 | 0.9814 | 2.99，0.98-9.16 | 100.00 |
| Nimbin (behind the medicine 2 days) | y＝1.5099x+5.1873 | 0.9849 | 0.75，0.46-1.22 | |
| Example 1: Verticillium lecanii: nimbin (100: 0.30) | y＝0.2904x+4.0621 | 0.9995 | 1.69，0.18-15.64 | 176.92 |
| Example 2: Verticillium lecanii: nimbin (100: 0.20) | y＝0.2702x+4.1991 | 0.9791 | 0.92，0.07-11.75 | 325.00 |
| Example 3: Verticillium lecanii: nimbin (100: 0.08) | y＝0.2697x+4.1776 | 0.9892 | 1.12，0.09-13.40 | 266.96 |
| Example 4: Verticillium lecanii: nimbin (100: 0.15) | y＝0.2704x+4.2259 | 0.9956 | 0.73，0.05-10.42 | 409.59 |
| Example 5: Verticillium lecanii: nimbin (100: 0.05) | y＝0.3042x+4.0147 | 0.9964 | 1.74，0.20-14.75 | 171.84 |
R
2: determination coefficient; TI: relative virus force index.
Claims (5)
1. a Verticillium lecanii (Verticillium lecanii) and the composite desinsection host of nimbin is characterized in that described host is the mixture of Verticillium lecanii and nimbin, and the mass ratio of described Verticillium lecanii and nimbin is 100: 0.05～0.30; Described verticillium lecanii strain VL-N6, bacterial strain preserving number: CCTCC No:M-208086.
2. according to described Verticillium lecanii of claim 1 and the composite desinsection host of nimbin, it is characterized in that described Verticillium lecanii and nimbin mass ratio are 100: 0.08～0.20.
3. an insecticide that is prepared by claim 1 or 2 described desinsection hosts is characterized in that being made up of host and assistant agent, and host is 20%～60% by mass percentage, and assistant agent is 40%～80%.
4. according to the described insecticide of claim 3, it is characterized in that described assistant agent is stabilizing agent, auxiliary agent and filler; Described stabilizing agent is phosphate buffer or the paraffin oil of glycerine, 0.21M; Described auxiliary agent is one or more mixtures in Tween-80, methyl green, the sodium lignin sulfonate; Described filler is kaolin, diatomite or potter's clay.
5. according to the described insecticide of claim 3, it is characterized in that described insecticide is wetting powder, suspending agent or finish.
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| CN101513194B true CN101513194B (en) | 2011-08-17 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102038001B (en) * | 2010-12-03 | 2014-12-31 | 成都绿金高新技术股份有限公司 | Azadirachtin and bacillus subtilis mixed water dispersible granule |
| CN106577776A (en) * | 2016-11-25 | 2017-04-26 | 河池市农业科学研究所 | Microbial pesticide for treating corn diseases |
| CN106719849B (en) * | 2017-01-20 | 2019-03-26 | 华南农业大学 | Insecticide associated with a kind of matrine and Verticillium lecanii |
| CN106912502B (en) * | 2017-05-02 | 2018-03-20 | 中国农业科学院植物保护研究所 | The compounded pesticides of Lecanicillium lecanii and jervine |
| CN106942281B (en) * | 2017-05-02 | 2018-03-20 | 中国农业科学院植物保护研究所 | The compounded pesticides of Lecanicillium lecanii and Celastrus angulatus |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101319193A (en) * | 2008-07-01 | 2008-12-10 | 华南农业大学 | Verticillium lecanii strain and uses thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101319193A (en) * | 2008-07-01 | 2008-12-10 | 华南农业大学 | Verticillium lecanii strain and uses thereof |
Non-Patent Citations (4)
| Title |
|---|
| J.Brodeur.Dose-response susceptibility of pest aphids (Homoptera Aphididae) and their control on hydroponically grown lettuce with the entomopathogenic fungus Verticillium lecanii, azadirachtin, and insecticidal soap.《Environmental Entomology》.2000,第29卷(第3期),568-578. |
| V.Fournier |
| V.Fournier;J.Brodeur.Dose-response susceptibility of pest aphids (Homoptera Aphididae) and their control on hydroponically grown lettuce with the entomopathogenic fungus Verticillium lecanii, azadirachtin, and insecticidal soap.《Environmental Entomology》.2000,第29卷(第3期),568-578. * |
| 陈霞,等.印楝素和蜡蚧轮枝菌对胡瓜钝绥螨的毒力测定.《福建农业学报》.2004,第19卷(第1期),202-205. * |
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