CN101497864B - Biological strain for improving tobacco quality - Google Patents
Biological strain for improving tobacco quality Download PDFInfo
- Publication number
- CN101497864B CN101497864B CN2009100941381A CN200910094138A CN101497864B CN 101497864 B CN101497864 B CN 101497864B CN 2009100941381 A CN2009100941381 A CN 2009100941381A CN 200910094138 A CN200910094138 A CN 200910094138A CN 101497864 B CN101497864 B CN 101497864B
- Authority
- CN
- China
- Prior art keywords
- percent
- tobacco
- test tube
- peptone
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a biological agent for improving the quality of tobacco, which belongs to the technical field of biology. The biological agent is prepared from the following procedures: a. strains of (Aspergillus foetidus) G01, CGMCCN0.2851 are produced; b. the strains of G01 are inoculated into an inclined culture medium of a test tube; the culture medium has a formulation of 1 percent of glucose, 0.5 percent of peptone, 1 percent of KH2PO4, 0.5 percent of MgSO4.7H2O, 2 percent of agaragar, 0.1 percent of chloromycetin and 0.03 percent of tetrachlorotetraiodofluorescein, and are cultured for two days under the environment of pH of 7.0 and the constant temperature of 30 DEG C so as to obtain test tube seeds; c. the test tube seeds are inoculated into a fluid nutrient medium of a 500ml triangular flask; each flask is filled with 200ml; the fluid nutrient medium has a formulation of 0.7 to1 percent of glucose, 0.3 to 0.5 percent of peptone, 0.5 to 1 percent of KH2PO4, 0.5 percent of MgSO4.7H2O, 0.1 percent of chloromycetin and 0.03 percent of tetrachlorotetraiodofluorescein, and are cultured through shaking under the environment of pH of 7.0 and temperature of 30 to 32 DEG C; the culture time is between 3 to 5 days; and the rotary speed is between 150 to 200r/min. When the cell concentration reaches that viable counts of per ml are more than or equal to 10 <7>, the fluid biological agent is obtained. The invention has the advantages that the biological agent can reduce sugar content of tobacco, improve potassium content, and improve the quality of tobacco. The invention can be widely applied to tobacco planting.
Description
Technical field:
The present invention relates to a kind of bacteria agent that improves quality of tobacco, belong to biological technical field.
Background technology:
Sugar content of tobacco is higher, potassium content is on the low side is the one of the main reasons that influences the Yunnan tobacco quality.Reducing sugar content of tobacco, increasing potassium content is one of field of greatest concern in the tobacco scientific research.Sugar content of tobacco is except that being subjected to inherited genetic factors, ecological factor influence, and uses special fertilising (execute ammonium fertilizer, execute competent phosphorus potash fertilizer), baking modulation (raising humidity) technology is to reduce the main path of sugar content of tobacco; Enriching potash fertilizer is the main path that improves potassium content in tobacco leaf.
Along with people to the extensive concern of smoking and health problem and reduce the rise of public hazards, protection environmental movement, how tobacco can satisfy the demands of consumers, and can reduce public hazards again, face serious challenge above the protection environment.At present, improve quality of tobacco, must suitably reduce sugar content of tobacco, increase potassium content.The microbial technique that research and development have an actual application value improves the tobacco leaf potassium content, suitably reduces sugared content, thereby improves quality of tobacco, has important practical significance and wide application prospect.
Summary of the invention:
Main purpose of the present invention is to provide a kind of can improve the quality of tobacco bacteria agent, provides technical support for producing sound tobacco.
The fungi that the present invention adopts is smelly aspergillus Aspergillus foetidus G01 bacterial strain, depositary institution: China Committee for Culture Collection of Microorganisms common micro-organisms center; Address: No. 1 institute in North Star West Road, Chaoyang District, BeiJing, China city; Preservation date: on January 8th, 2009; The numbering CGMCC NO.2851 that preservation is registered on the books.
The G01 bacterial strain belongs to soil fungi, be that the inventor screens from a large amount of soil bacteria bacterial strains, ability with the quality of tobacco of significantly improving, identify that through Chinese Academy of Agricultural Sciences Chinese agriculture microbial strains preservation administrative center (ACCC) the G01 bacterial strain is smelly aspergillus Aspergillusfoetidus.
The present invention is achieved in that the test tube seed culture of G01 bacterial strain, and the shaking table enlarged culturing is prepared as biotechnological formulation, and preparation is applied in the tobacco-growing soil of land for growing field crops, and potassium content in tobacco leaf is measured in the ripe modulation of tobacco leaf back.
The cultural method of bacterium G01 bacterial strain of the present invention (below be weight percentage):
1, test tube seed culture
The test tube slant culture medium prescription is: glucose 1%, peptone 0.5%, KH
2PO
41%, MgSO
4.7H
2O0.5%, agar 2%, paraxin 0.1%, brave red sodium salt 0.03%, pH 7.0.
To the test tube slant substratum, 30 ℃ of constant temperature culture 2 days obtain the test tube seed with the G01 inoculation.
2, liquid fermentation and culture
The liquid fermentation medium prescription is: glucose 0.7～1%, peptone 0.3～0.5%, KH
2PO
40.5 MgSO～1%,
4.7H
2O 0.5%, paraxin 0.1%, brave red sodium salt 0.03%, pH 7.0.
In 500m l triangular flask liquid nutrient medium, every bottled 200ml cultivates in 30～32 ℃ of shaking tables with test tube kind inoculation, incubation time 3～5 days, rotating speed is 150～200r/min, measures bacteria suspension concentration with the dilution plate counting process behind the shaking table under the nutrient solution, and bacteria concentration is every ml viable count 〉=10
7Promptly get and have the biotechnological formulation that improves the potassium content in tobacco leaf function.
Biotechnological formulation of the present invention improves the potassium content in tobacco leaf function test:
1, preparation biotechnological formulation: by the cultural method preparation test preparation of aforementioned G01 bacterial strain; Handle in contrast with the liquid nutrient medium of not inoculating the G01 bacterial strain.
2, biotechnological formulation is to method for treatment of tobacco
Flue-cured tobacco K326 kind (the 7 gram/strains of purity nitrogen amount of application, N: P
2O
5: K
2O=1: 1: 3), behind the cigarette seedling field-transplanting 15 days, will water for the every strain of examination microbial inoculum and execute 100 milliliters, all the other are by high-quality cigarette production technology Routine Management, and baking modulation is routinely plucked in the leaflet position after the tobacco leaf maturation.
3, tobacco leaf potassium content measuring method
Total reducing sugar and reducing sugar are measured by " the mensuration continuous flow method of tobacco and tobacco product water-soluble sugar " YC/T159～2002 methods.Total reducing sugar (reducing sugar) content reduces percentile method of calculation and is:
Total reducing sugar (reducing sugar) content reduces percentage %=(control treatment sugar content of tobacco-preparation is handled sugar content of tobacco)/control treatment sugar content of tobacco * 100
The tobacco leaf potassium content is measured by " the mensuration flame photometry of tobacco and tobacco product potassium " YC/T 173～2003 methods.The tobacco leaf potassium content improves percentile method of calculation:
Potassium content improves percentage %=(preparation is handled tobacco leaf potassium content-control treatment tobacco leaf potassium content)/control treatment tobacco leaf potassium content * 100
4, test-results
Table 1 biotechnological formulation is handled tobacco leaf sugar and potassium content measurement result
Leaf position: up count numbering from first pin leaf of cigarette strain.Annotate: data are analyzed by Institute of Analysis of Yunnan Provine Tobacco Science Inst..
The result shows, the 5th, 10, the 14 leaf position tobacco leaf total sugar contents that biotechnological formulation is handled are compared respectively according to reducing by 27.47%, 22.07%, 9.49%, reducing sugar content is compared respectively according to reducing by 25.91%, 20.34%, 7.64%, potassium content is compared respectively according to improving 7.01%, 20.22%, 23.72%, show that biotechnological formulation of the present invention has the sugar content of tobacco of reduction, improves the tobacco leaf potassium content, thereby improve quality of tobacco.
Biotechnological formulation of the present invention has the sugar content of tobacco of reduction, improves the tobacco leaf potassium content, thereby improves the advantage of quality of tobacco, can be widely used in the plantation of tobacco leaf.
Embodiment:
Be described in further detail the present invention with embodiment below, but content of the present invention is not limited thereto.
Embodiment 1:
To the test tube agar slant, culture medium prescription is with Aspergillus foetidus G01 inoculation: glucose 1%, peptone 0.5%, KH
2PO
41%, MgSO
4.7H
2O 0.5%, agar 2%, paraxin 0.1%, brave red sodium salt 0.03%, pH cultivated constant temperature culture 2 days for 7.0,30 ℃, obtained the test tube kind.
The test tube kind is inoculated in 500ml triangular flask (the every bottled 200ml) liquid nutrient medium, and culture medium prescription is: glucose 1%, peptone 0.5%, KH
2PO
41%, MgSO
4.7H
2O 0.5%, paraxin 0.1%, brave red sodium salt 0.03%, pH 7.0.Cultivate in 30 ℃ of following shaking tables (rotating speed is 150r/min) connecting the triangular flask of planting, incubation time 3 days treats that bacteria concentration is for reaching every ml viable count 〉=10
7The time, promptly get biotechnological formulation with raising quality of tobacco function.This bacteria agent watered for 100 milliliters by every strain execute tobacco leaf, can reduce sugar content of tobacco, improve the tobacco leaf potassium content, thereby improve quality of tobacco.
Embodiment 2:
Substantially with embodiment one, difference is the liquid culture based formulas, and its prescription is: glucose 0.7%, peptone 0.3%, KH
2PO
40.5%.Cultivate incubation time 5 days in 32 ℃ of following shaking tables (rotating speed is 200r/min) with connecting the triangular flask of planting.
Embodiment 3:
Substantially with embodiment one, difference is the liquid culture based formulas, and its prescription is: glucose 0.8%, peptone 0.4%, KH
2PO
40.8%.Cultivate incubation time 4 days in 31 ℃ of following shaking tables (rotating speed is 180r/min) with connecting the triangular flask of planting.
Claims (1)
1. bacteria agent that improves quality of tobacco is characterized in that this bacteria agent by producing bacterial strain through test tube seed culture, the preparation of shaking table enlarged culturing operation, is characterized in that:
A. producing bacterial strain is smelly aspergillus (Aspergillus foetidus) G01, CGMCC NO.2851;
B. test tube seed culture: to the test tube slant substratum, culture medium prescription is glucose 1%, peptone 0.5%, KH with the G01 inoculation
2PO
41%, MgSO
4.7H
2O 0.5%, agar 2%, paraxin 0.1%, brave red sodium salt 0.03%, pH 7.0, and the ratio of prescription is weight percentage, and 30 ℃ of constant temperature culture 2 days obtain the test tube seed;
C. shaking table enlarged culturing: the test tube seed is inoculated in the 500ml triangular flask liquid nutrient medium, and every bottled 200ml, liquid fermentation medium prescription are glucose 0.7～1%, peptone 0.3～0.5%, KH
2PO
40.5 MgSO～1%,
4.7H
2O 0.5%, paraxin 0.1%, brave red sodium salt 0.03%, pH 7.0, the ratio of prescription is weight percentage, and cultivates in 30～32 ℃ of shaking tables, and incubation time 3～5 days, rotating speed are 150～200rpm, when bacteria concentration is every ml viable count 〉=10
7Obtain biotechnological formulation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100941381A CN101497864B (en) | 2009-02-27 | 2009-02-27 | Biological strain for improving tobacco quality |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100941381A CN101497864B (en) | 2009-02-27 | 2009-02-27 | Biological strain for improving tobacco quality |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101497864A CN101497864A (en) | 2009-08-05 |
| CN101497864B true CN101497864B (en) | 2010-09-29 |
Family
ID=40945136
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009100941381A Expired - Fee Related CN101497864B (en) | 2009-02-27 | 2009-02-27 | Biological strain for improving tobacco quality |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101497864B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613695B (en) * | 2012-03-28 | 2014-02-26 | 华宝食用香精香料（上海）有限公司 | Method utilizing functional liquid to reduce content of carbohydrate materials in tobaccos |
| CN102618449B (en) * | 2012-04-01 | 2014-05-28 | 云南省微生物发酵工程研究中心有限公司 | Phosphate solubilizing bacterium, as well as preparation method and application thereof |
-
2009
- 2009-02-27 CN CN2009100941381A patent/CN101497864B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN101497864A (en) | 2009-08-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105255769B (en) | One Enterobacter cloacae and its application | |
| CN103275895B (en) | Saline-alkali-tolerant heteroauxin-producing Bacillus subtilis and application thereof | |
| CN102391960B (en) | Arthrobacter chlorophenolicus L4 and application thereof | |
| CN106591205B (en) | One plant of acinetobacter bacterium NJAU-3 for having phosphorus decomposing growth-promoting ability and its application | |
| CN106434471B (en) | A kind of microbial composite and purposes promoting tobacco growing | |
| CN105316255B (en) | A kind of method of soil beneficial microbe mixed fermentation | |
| CN103374528B (en) | Aspergillus niger strain and application thereof | |
| CN103243055B (en) | Salt/alkali-tolerant heteroauxin-producing bacterium strain with fluoranthene degradation capacity and application thereof | |
| WO2023115830A1 (en) | Complex microbial agent for promoting growth of soybeans and improving population abundance of probiotic microorganisms | |
| CN104630087B (en) | A kind of Rhizosphere of Crops Promoting bacteria YM4 and its application | |
| WO2021057441A1 (en) | Rhizobium, microbacterium sp. x-18 and application thereof | |
| CN102154194A (en) | Preparation method for high-yield chlamydospore liquid by virtue of fermentation of trichoderma on pilot plant test scale | |
| CN109456915A (en) | One seed sand good fortune bacillus strain X3 and its application | |
| CN108795794B (en) | Bacillus pumilus, screening method thereof, preparation method of microbial inoculum and application of microbial inoculum | |
| CN104630090B (en) | A kind of Rhizosphere of Crops Promoting bacteria YM3 and its application | |
| CN103243059B (en) | Heteroauxin-producing Arthrobacter pascens strain with fluoranthene degradation capacity and application thereof | |
| CN103952332B (en) | A kind of bacillus amyloliquefaciens ZH1 and application thereof | |
| CN104630094A (en) | Peanut growth-promoting rhizobacterium and application thereof | |
| CN109516869A (en) | Complex function microorganism formulation and its preparation method and application | |
| CN101497864B (en) | Biological strain for improving tobacco quality | |
| CN102747006B (en) | Potassium decomposition bacteria, preparation method and application thereof | |
| CN102584453B (en) | Composite microorganism bacterial manure and preparation method thereof | |
| CN102226161A (en) | Preparation method for burkholderia cepacia, and application in high-performance phosphate-dissolving microbial fertilizer by using the same | |
| CN109486709B (en) | One plant of Fan Shi Halomonas bacterial strain JPT10-1 for improving corn salt resistance ability and its microbial inoculum and application | |
| CN101485334B (en) | Biological microbial formulation for increasing potassium content in tobacco leaf |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100929 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |