CN101496834A - Preparation method of mulberry twig flavone and diphenyl ethylene extract - Google Patents
Preparation method of mulberry twig flavone and diphenyl ethylene extract Download PDFInfo
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- CN101496834A CN101496834A CNA2009100958382A CN200910095838A CN101496834A CN 101496834 A CN101496834 A CN 101496834A CN A2009100958382 A CNA2009100958382 A CN A2009100958382A CN 200910095838 A CN200910095838 A CN 200910095838A CN 101496834 A CN101496834 A CN 101496834A
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Abstract
The invention relates to a method for preparing a ramulus mori flavone and diphenyl ethylene extract. The ramulus mori flavone and diphenyl ethylene extract is obtained by a hydroalcoholic method and elution through a macroporous polymeric adsorbent column. The content of diphenyl ethylene compounds is between 30 and 70 weight percent and the content of flavone compounds is between 30 and 70 weight percent as measured by ultraviolet spectrophotometry and an HPLC method by adoption of mulberroside and Quercetin-3-O-beta-D-mannopyranoside as index compositions. The invention provides a novel method for preparing the ramulus mori flavone and diphenyl ethylene extract.
Description
Technical field:
The present invention relates to the preparation method of mulberry twig flavone and diphenyl ethylene extract.
The background technology summary of the invention::
Ramulus Mori (Ramulus mori) is the annual dry twig of moraceae plants Mulberry (Morus alba L.), as Chinese herbal medicine commonly used among the people, Chinese Pharmacopoeia record Ramulus Mori has the effect of wind-damp dispelling, sharp joint, row water, is applicable to the disease of anemofrigid-damp arthralgia, spasm of the limbs, beriberi edema.Its contained chemical constituent kind is more, mainly contains polysaccharide, flavone compound, coumarin kind compound, alkaloid, contains volatile oil, aminoacid, organic acid and various vitamin etc. in addition.Other has in the report Ramulus Mori contains the diphenylethylene compounds mulberry skin glucoside.Though Ramulus Mori has clinical preferably basis, never find the suitable preparation mulberry twig flavone wherein and the method for diphenyl ethylene extract.
Summary of the invention:
The preparation method that the objective of the invention is to open mulberry twig flavone and diphenyl ethylene extract.
Technical solution of the present invention is: Ramulus Mori passes through water alcohol method, mulberry twig flavone that the macroporous adsorptive resins eluting obtains and diphenyl ethylene extract, to be reference substance record flavone compound weight ratio content respectively through ultraviolet spectrophotometry and HPLC method with Quercetin-3-O-β-D-mannopyranoside is 30%-70%; To be reference substance with the mulberry skin glucoside record diphenylethylene compounds weight ratio content respectively through ultraviolet spectrophotometry and HPLC method is 30%-70%.
The preparation of mulberry twig flavone and diphenyl ethylene extract:
(1) get dry Ramulus Mori, hot reflux three times adds 7 times of 60% ethanol at every turn, and each 2 hours, merge extractive liquid, filtered, and filtrate is contained the 0.5g medical material through being evaporated to every ml medicinal liquid, puts coldly, filters, and discards medicinal residues.
(2) get filtrate, by flow velocity is the good macroporous adsorptive resins of pretreatment on 1-4BV/h, distilled water eluting with 2-6 times of amount bed volumes, flow velocity is 1--4BV/h, discards water elution liquid, presses the speed eluting of 1-4BV/h again with 60% ethanol of 2-6 times of amount bed volumes, collect eluent, reclaim ethanol and be concentrated into thick paste, drying under reduced pressure gets dry extract.
(3) with the mulberry skin glucoside be reference substance, measure diphenylethylene compounds weight ratio content 30%-70% in mulberry twig flavone and the diphenyl ethylene extract according to the pharmacopeia method.
(4) being reference substance with Quercetin-3-O-β-D-mannopyranoside, is 30%-70% according to flavone compound weight ratio content in pharmacopeia method mensuration mulberry twig flavone and the diphenyl ethylene extract.
Macroporous adsorptive resins is preferably HPD-600 type macroporous adsorptive resins.
Mulberry twig flavone and diphenyl ethylene extract can be applied in the preparation medicament for inhibiting uric acid reabsorption transporter.This new purposes has comprised with mulberry twig flavone of the present invention and diphenyl ethylene extract, be equipped with pharmaceutic adjuvant commonly used (as: excipient, cosolvent, controlled release agent), make various dosage forms and comprise oral liquid, injection, capsule, tablet, granule, microcapsule, be used to prepare medicament for inhibiting uric acid reabsorption transporter.
Advantage of the present invention is that the preparation method of mulberry twig flavone and diphenyl ethylene extract is provided.
The specific embodiment:
Embodiment 1:
(1) get dry Ramulus Mori, hot reflux three times adds 7 times of 60% ethanol at every turn, and each 2 hours, merge extractive liquid, filtered, and filtrate is contained the 0.5g medical material through being evaporated to every ml medicinal liquid, puts coldly, filters, and discards medicinal residues.
(2) get filtrate, by flow velocity is the HPD-600 type macroporous adsorptive resins that pretreatment is good on the 1BV/h, and with the distilled water eluting of 2 times of amount bed volumes, flow velocity is 1BV/h, discard water elution liquid, press the speed eluting of 1BV/h again with 60% ethanol of 6 times of amount bed volumes, collect eluent, reclaim ethanol and be concentrated into thick paste, drying under reduced pressure, get dry extract, be ground into fine powder, promptly get mulberry twig flavone and diphenyl ethylene extract.
(3) with the mulberry skin glucoside be reference substance, measure diphenylethylene compounds weight ratio content 30%-70% in mulberry twig flavone and the diphenyl ethylene extract according to the pharmacopeia method.
(4) being reference substance with Quercetin-3-O-β-D-mannopyranoside, is 30%-70% according to flavone compound weight ratio content in pharmacopeia method mensuration mulberry twig flavone and the diphenyl ethylene extract.
Embodiment 2:
(1) get dry Ramulus Mori, hot reflux three times adds 7 times of 60% ethanol at every turn, and each 2 hours, merge extractive liquid, filtered, and filtrate is contained the 0.5g medical material through being evaporated to every ml medicinal liquid, puts coldly, filters, and discards medicinal residues.
(2) get filtrate, by flow velocity is the HPD-600 type macroporous adsorptive resins that pretreatment is good on the 2BV/h, and with the distilled water eluting of 2 times of amount bed volumes, flow velocity is 2BV/h, discard water elution liquid, press the speed eluting of 2BV/h again with 60% ethanol of 5 times of amount bed volumes, collect eluent, reclaim ethanol and be concentrated into thick paste, drying under reduced pressure, get dry extract, be ground into fine powder, promptly get mulberry twig flavone and diphenyl ethylene extract.
(3) with the mulberry skin glucoside be reference substance, measure diphenylethylene compounds weight ratio content 30%-70% in mulberry twig flavone and the diphenyl ethylene extract according to the pharmacopeia method.
(4) being reference substance with Quercetin-3-O-β-D-mannopyranoside, is 30%-70% according to flavone compound weight ratio content in pharmacopeia method mensuration mulberry twig flavone and the diphenyl ethylene extract.
Embodiment 3:
(1) get dry Ramulus Mori, hot reflux three times adds 7 times of 60% ethanol at every turn, and each 2 hours, merge extractive liquid, filtered, and filtrate is contained the 0.5g medical material through being evaporated to every ml medicinal liquid, puts coldly, filters, and discards medicinal residues.
(2) get filtrate, by flow velocity is the HPD-600 type macroporous adsorptive resins that pretreatment is good on the 3BV/h, and with the distilled water eluting of 2 times of amount bed volumes, flow velocity is 3BV/h, discard water elution liquid, press the speed eluting of 3BV/h again with 60% ethanol of 4 times of amount bed volumes, collect eluent, reclaim ethanol and be concentrated into thick paste, drying under reduced pressure, get dry extract, be ground into fine powder, promptly get mulberry twig flavone and diphenyl ethylene extract.
(3) with the mulberry skin glucoside be reference substance, measure diphenylethylene compounds weight ratio content 30%-70% in mulberry twig flavone and the diphenyl ethylene extract according to the pharmacopeia method.
(4) being reference substance with Quercetin-3-O-β-D-mannopyranoside, is 30%-70% according to flavone compound weight ratio content in pharmacopeia method mensuration mulberry twig flavone and the diphenyl ethylene extract.
Embodiment 4:
(1) get dry Ramulus Mori, hot reflux three times adds 7 times of 60% ethanol at every turn, and each 2 hours, merge extractive liquid, filtered, and filtrate is contained the 0.5g medical material through being evaporated to every ml medicinal liquid, puts coldly, filters, and discards medicinal residues.
(2) get filtrate, by flow velocity is the HPD-600 type macroporous adsorptive resins that pretreatment is good on the 4BV/h, and with the distilled water eluting of 3 times of amount bed volumes, flow velocity is 4BV/h, discard water elution liquid, press the speed eluting of 4BV/h again with 60% ethanol of 3 times of amount bed volumes, collect eluent, reclaim ethanol and be concentrated into thick paste, drying under reduced pressure, get dry extract, be ground into fine powder, promptly get mulberry twig flavone and diphenyl ethylene extract.
(3) with the mulberry skin glucoside be reference substance, measure diphenylethylene compounds weight ratio content 30%-70% in mulberry twig flavone and the diphenyl ethylene extract according to the pharmacopeia method.
(4) being reference substance with Quercetin-3-O-β-D-mannopyranoside, is 30%-70% according to flavone compound weight ratio content in pharmacopeia method mensuration mulberry twig flavone and the diphenyl ethylene extract.
Embodiment 5:
(1) get dry Ramulus Mori, hot reflux three times adds 7 times of 60% ethanol at every turn, and each 2 hours, merge extractive liquid, filtered, and filtrate is contained the 0.5g medical material through being evaporated to every ml medicinal liquid, puts coldly, filters, and discards medicinal residues.
(2) get filtrate, by flow velocity is the HPD-600 type macroporous adsorptive resins that pretreatment is good on the 2BV/h, and with the distilled water eluting of 3 times of amount bed volumes, flow velocity is 3BV/h, discard water elution liquid, press the speed eluting of 4BV/h again with 60% ethanol of 2 times of amount bed volumes, collect eluent, reclaim ethanol and be concentrated into thick paste, drying under reduced pressure, get dry extract, be ground into fine powder, promptly get mulberry twig flavone and diphenyl ethylene extract.
(3) with the mulberry skin glucoside be reference substance, measure diphenylethylene compounds weight ratio content 30%-70% in mulberry twig flavone and the diphenyl ethylene extract according to the pharmacopeia method.
(4) being reference substance with Quercetin-3-O-β-D-mannopyranoside, is 30%-70% according to flavone compound weight ratio content in pharmacopeia method mensuration mulberry twig flavone and the diphenyl ethylene extract.
Embodiment 6:
(1) get dry Ramulus Mori, hot reflux three times adds 7 times of 60% ethanol at every turn, and each 2 hours, merge extractive liquid, filtered, and filtrate is contained the 0.5g medical material through being evaporated to every ml medicinal liquid, puts coldly, filters, and discards medicinal residues.
(2) get filtrate, by flow velocity is the HPD-600 type macroporous adsorptive resins that pretreatment is good on the 1BV/h, and with the distilled water eluting of 3 times of amount bed volumes, flow velocity is 4BV/h, discard water elution liquid, press the speed eluting of 3BV/h again with 60% ethanol of 6 times of amount bed volumes, collect eluent, reclaim ethanol and be concentrated into thick paste, drying under reduced pressure, get dry extract, be ground into fine powder, promptly get mulberry twig flavone and diphenyl ethylene extract.
(3) with the mulberry skin glucoside be reference substance, measure diphenylethylene compounds weight ratio content 30%-70% in mulberry twig flavone and the diphenyl ethylene extract according to the pharmacopeia method.
(4) being reference substance with Quercetin-3-O-β-D-mannopyranoside, is 30%-70% according to flavone compound weight ratio content in pharmacopeia method mensuration mulberry twig flavone and the diphenyl ethylene extract.
Embodiment 7:
(1) get dry Ramulus Mori, hot reflux three times adds 7 times of 60% ethanol at every turn, and each 2 hours, merge extractive liquid, filtered, and filtrate is contained the 0.5g medical material through being evaporated to every ml medicinal liquid, puts coldly, filters, and discards medicinal residues.
(2) get filtrate, by flow velocity is the HPD-600 type macroporous adsorptive resins that pretreatment is good on the 4BV/h, and with the distilled water eluting of 4 times of amount bed volumes, flow velocity is 1BV/h, discard water elution liquid, press the speed eluting of 2BV/h again with 60% ethanol of 5 times of amount bed volumes, collect eluent, reclaim ethanol and be concentrated into thick paste, drying under reduced pressure, get dry extract, be ground into fine powder, promptly get mulberry twig flavone and diphenyl ethylene extract.
(3) with the mulberry skin glucoside be reference substance, measure diphenylethylene compounds weight ratio content 30%-70% in mulberry twig flavone and the diphenyl ethylene extract according to the pharmacopeia method.
(4) being reference substance with Quercetin-3-O-β-D-mannopyranoside, is 30%-70% according to flavone compound weight ratio content in pharmacopeia method mensuration mulberry twig flavone and the diphenyl ethylene extract.
Embodiment 8:
(1) get dry Ramulus Mori, hot reflux three times adds 7 times of 60% ethanol at every turn, and each 2 hours, merge extractive liquid, filtered, and filtrate is contained the 0.5g medical material through being evaporated to every ml medicinal liquid, puts coldly, filters, and discards medicinal residues.
(2) get filtrate, by flow velocity is the HPD-600 type macroporous adsorptive resins that pretreatment is good on the 3BV/h, and with the distilled water eluting of 4 times of amount bed volumes, flow velocity is 2BV/h, discard water elution liquid, press the speed eluting of 1BV/h again with 60% ethanol of 4 times of amount bed volumes, collect eluent, reclaim ethanol and be concentrated into thick paste, drying under reduced pressure, get dry extract, be ground into fine powder, promptly get mulberry twig flavone and diphenyl ethylene extract.
(3) with the mulberry skin glucoside be reference substance, measure diphenylethylene compounds weight ratio content 30%-70% in mulberry twig flavone and the diphenyl ethylene extract according to the pharmacopeia method.
(4) being reference substance with Quercetin-3-O-β-D-mannopyranoside, is 30%-70% according to flavone compound weight ratio content in pharmacopeia method mensuration mulberry twig flavone and the diphenyl ethylene extract.
Embodiment 9:
(1) get dry Ramulus Mori, hot reflux three times adds 7 times of 60% ethanol at every turn, and each 2 hours, merge extractive liquid, filtered, and filtrate is contained the 0.5g medical material through being evaporated to every ml medicinal liquid, puts coldly, filters, and discards medicinal residues.
(2) get filtrate, by flow velocity is the HPD-600 type macroporous adsorptive resins that pretreatment is good on the 4BV/h, and with the distilled water eluting of 4 times of amount bed volumes, flow velocity is 1BV/h, discard water elution liquid, press the speed eluting of 3BV/h again with 60% ethanol of 3 times of amount bed volumes, collect eluent, reclaim ethanol and be concentrated into thick paste, drying under reduced pressure, get dry extract, be ground into fine powder, promptly get mulberry twig flavone and diphenyl ethylene extract.
(3) with the mulberry skin glucoside be reference substance, measure diphenylethylene compounds weight ratio content 30%-70% in mulberry twig flavone and the diphenyl ethylene extract according to the pharmacopeia method.
(4) being reference substance with Quercetin-3-O-β-D-mannopyranoside, is 30%-70% according to flavone compound weight ratio content in pharmacopeia method mensuration mulberry twig flavone and the diphenyl ethylene extract.
Embodiment 10:
(1) get dry Ramulus Mori, hot reflux three times adds 7 times of 60% ethanol at every turn, and each 2 hours, merge extractive liquid, filtered, and filtrate is contained the 0.5g medical material through being evaporated to every ml medicinal liquid, puts coldly, filters, and discards medicinal residues.
(2) get filtrate, by flow velocity is the HPD-600 type macroporous adsorptive resins that pretreatment is good on the 3BV/h, and with the distilled water eluting of 5 times of amount bed volumes, flow velocity is 2BV/h, discard water elution liquid, press the speed eluting of 4BV/h again with 60% ethanol of 2 times of amount bed volumes, collect eluent, reclaim ethanol and be concentrated into thick paste, drying under reduced pressure, get dry extract, be ground into fine powder, promptly get mulberry twig flavone and diphenyl ethylene extract.
(3) with the mulberry skin glucoside be reference substance, measure diphenylethylene compounds weight ratio content 30%-70% in mulberry twig flavone and the diphenyl ethylene extract according to the pharmacopeia method.
(4) being reference substance with Quercetin-3-O-β-D-mannopyranoside, is 30%-70% according to flavone compound weight ratio content in pharmacopeia method mensuration mulberry twig flavone and the diphenyl ethylene extract.
Embodiment 11:
(1) get dry Ramulus Mori, hot reflux three times adds 7 times of 60% ethanol at every turn, and each 2 hours, merge extractive liquid, filtered, and filtrate is contained the 0.5g medical material through being evaporated to every ml medicinal liquid, puts coldly, filters, and discards medicinal residues.
(2) get filtrate, by flow velocity is the HPD-600 type macroporous adsorptive resins that pretreatment is good on the 2BV/h, and with the distilled water eluting of 5 times of amount bed volumes, flow velocity is 3BV/h, discard water elution liquid, press the speed eluting of 1BV/h again with 60% ethanol of 6 times of amount bed volumes, collect eluent, reclaim ethanol and be concentrated into thick paste, drying under reduced pressure, get dry extract, be ground into fine powder, promptly get mulberry twig flavone and diphenyl ethylene extract.
(3) with the mulberry skin glucoside be reference substance, measure diphenylethylene compounds weight ratio content 30%-70% in mulberry twig flavone and the diphenyl ethylene extract according to the pharmacopeia method.
(4) being reference substance with Quercetin-3-O-β-D-mannopyranoside, is 30%-70% according to flavone compound weight ratio content in pharmacopeia method mensuration mulberry twig flavone and the diphenyl ethylene extract.
Embodiment 12:
(1) get dry Ramulus Mori, hot reflux three times adds 7 times of 60% ethanol at every turn, and each 2 hours, merge extractive liquid, filtered, and filtrate is contained the 0.5g medical material through being evaporated to every ml medicinal liquid, puts coldly, filters, and discards medicinal residues.
(2) get filtrate, by flow velocity is the HPD-600 type macroporous adsorptive resins that pretreatment is good on the 1BV/h, and with the distilled water eluting of 5 times of amount bed volumes, flow velocity is 4BV/h, discard water elution liquid, press the speed eluting of 2BV/h again with 60% ethanol of 5 times of amount bed volumes, collect eluent, reclaim ethanol and be concentrated into thick paste, drying under reduced pressure, get dry extract, be ground into fine powder, promptly get mulberry twig flavone and diphenyl ethylene extract.
(3) with the mulberry skin glucoside be reference substance, measure diphenylethylene compounds weight ratio content 30%-70% in mulberry twig flavone and the diphenyl ethylene extract according to the pharmacopeia method.
(4) being reference substance with Quercetin-3-O-β-D-mannopyranoside, is 30%-70% according to flavone compound weight ratio content in pharmacopeia method mensuration mulberry twig flavone and the diphenyl ethylene extract.
Embodiment 13:
(1) get dry Ramulus Mori, hot reflux three times adds 7 times of 60% ethanol at every turn, and each 2 hours, merge extractive liquid, filtered, and filtrate is contained the 0.5g medical material through being evaporated to every ml medicinal liquid, puts coldly, filters, and discards medicinal residues.
(2) get filtrate, by flow velocity is the HPD-600 type macroporous adsorptive resins that pretreatment is good on the 3BV/h, and with the distilled water eluting of 6 times of amount bed volumes, flow velocity is 3BV/h, discard water elution liquid, press the speed eluting of 2BV/h again with 60% ethanol of 4 times of amount bed volumes, collect eluent, reclaim ethanol and be concentrated into thick paste, drying under reduced pressure, get dry extract, be ground into fine powder, promptly get mulberry twig flavone and diphenyl ethylene extract.
(3) with the mulberry skin glucoside be reference substance, measure diphenylethylene compounds weight ratio content 30%-70% in mulberry twig flavone and the diphenyl ethylene extract according to the pharmacopeia method.
(4) being reference substance with Quercetin-3-O-β-D-mannopyranoside, is 30%-70% according to flavone compound weight ratio content in pharmacopeia method mensuration mulberry twig flavone and the diphenyl ethylene extract.
Embodiment 14:
(1) get dry Ramulus Mori, hot reflux three times adds 7 times of 60% ethanol at every turn, and each 2 hours, merge extractive liquid, filtered, and filtrate is contained the 0.5g medical material through being evaporated to every ml medicinal liquid, puts coldly, filters, and discards medicinal residues.
(2) get filtrate, by flow velocity is the HPD-600 type macroporous adsorptive resins that pretreatment is good on the 4BV/h, and with the distilled water eluting of 6 times of amount bed volumes, flow velocity is 4BV/h, discard water elution liquid, press the speed eluting of 1BV/h again with 60% ethanol of 3 times of amount bed volumes, collect eluent, reclaim ethanol and be concentrated into thick paste, drying under reduced pressure, get dry extract, be ground into fine powder, promptly get mulberry twig flavone and diphenyl ethylene extract.
(3) with the mulberry skin glucoside be reference substance, measure diphenylethylene compounds weight ratio content 30%-70% in mulberry twig flavone and the diphenyl ethylene extract according to the pharmacopeia method.
(4) being reference substance with Quercetin-3-O-β-D-mannopyranoside, is 30%-70% according to flavone compound weight ratio content in pharmacopeia method mensuration mulberry twig flavone and the diphenyl ethylene extract.
Embodiment 15:
(1) get dry Ramulus Mori, hot reflux three times adds 7 times of 60% ethanol at every turn, and each 2 hours, merge extractive liquid, filtered, and filtrate is contained the 0.5g medical material through being evaporated to every ml medicinal liquid, puts coldly, filters, and discards medicinal residues.
(2) get filtrate, by flow velocity is the HPD-600 type macroporous adsorptive resins that pretreatment is good on the 1BV/h, and with the distilled water eluting of 6 times of amount bed volumes, flow velocity is 1BV/h, discard water elution liquid, press the speed eluting of 4BV/h again with 60% ethanol of 2 times of amount bed volumes, collect eluent, reclaim ethanol and be concentrated into thick paste, drying under reduced pressure, get dry extract, be ground into fine powder, promptly get mulberry twig flavone and diphenyl ethylene extract.
(3) with the mulberry skin glucoside be reference substance, measure diphenylethylene compounds weight ratio content 30%-70% in mulberry twig flavone and the diphenyl ethylene extract according to the pharmacopeia method.
(4) being reference substance with Quercetin-3-O-β-D-mannopyranoside, is 30%-70% according to flavone compound weight ratio content in pharmacopeia method mensuration mulberry twig flavone and the diphenyl ethylene extract.
Embodiment 16:
(1) get dry Ramulus Mori, hot reflux three times adds 7 times of 60% ethanol at every turn, and each 2 hours, merge extractive liquid, filtered, and filtrate is contained the 0.5g medical material through being evaporated to every ml medicinal liquid, puts coldly, filters, and discards medicinal residues.
(2) get filtrate, by flow velocity is the HPD-600 type macroporous adsorptive resins that pretreatment is good on the 2BV/h, and with the distilled water eluting of 2 times of amount bed volumes, flow velocity is 2BV/h, discard water elution liquid, press the speed eluting of 3BV/h again with 60% ethanol of 6 times of amount bed volumes, collect eluent, reclaim ethanol and be concentrated into thick paste, drying under reduced pressure, get dry extract, be ground into fine powder, promptly get mulberry twig flavone and diphenyl ethylene extract.
(3) with the mulberry skin glucoside be reference substance, measure diphenylethylene compounds weight ratio content 30%-70% in mulberry twig flavone and the diphenyl ethylene extract according to the pharmacopeia method.
(4) being reference substance with Quercetin-3-O-β-D-mannopyranoside, is 30%-70% according to flavone compound weight ratio content in pharmacopeia method mensuration mulberry twig flavone and the diphenyl ethylene extract.
Claims (2)
1. the preparation method of mulberry twig flavone and diphenyl ethylene extract is characterized in that described preparation method may further comprise the steps:
A. get dry Ramulus Mori, hot reflux three times adds 7 times of 60% ethanol at every turn, and each 2 hours, merge extractive liquid, filtered, and filtrate is contained the 0.5g medical material through being evaporated to every m1 medicinal liquid, puts coldly, filters, and discards medicinal residues;
B. get filtrate, by flow velocity is the good macroporous adsorptive resins of pretreatment on 1-4BV/h, distilled water eluting with 2-6 times of amount bed volumes, flow velocity is 1--4BV/h, discards water elution liquid, presses the speed eluting of 1-4BV/h again with 60% ethanol of 2-6 times of amount bed volumes, collect eluent, reclaim ethanol and be concentrated into thick paste, drying under reduced pressure gets dry extract.
2. the preparation method of mulberry twig flavone according to claim 1 and diphenyl ethylene extract is characterized in that, macroporous adsorptive resins is a HPD-600 type macroporous adsorptive resins.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100958382A CN101496834A (en) | 2009-02-12 | 2009-02-12 | Preparation method of mulberry twig flavone and diphenyl ethylene extract |
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2009100958382A CN101496834A (en) | 2009-02-12 | 2009-02-12 | Preparation method of mulberry twig flavone and diphenyl ethylene extract |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103159807A (en) * | 2013-03-27 | 2013-06-19 | 苏州大学 | Ramulus mori extract and preparation method thereof |
| CN108395461A (en) * | 2017-02-07 | 2018-08-14 | 浙江康恩贝制药股份有限公司 | 7,4 '-O- β-D-Glucose morin glycosides and its preparation and its application in preparing anti-inflammatory drug |
-
2009
- 2009-02-12 CN CNA2009100958382A patent/CN101496834A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103159807A (en) * | 2013-03-27 | 2013-06-19 | 苏州大学 | Ramulus mori extract and preparation method thereof |
| CN103159807B (en) * | 2013-03-27 | 2015-08-05 | 苏州大学 | A kind of Ramulus Mori extract and preparation method thereof |
| CN108395461A (en) * | 2017-02-07 | 2018-08-14 | 浙江康恩贝制药股份有限公司 | 7,4 '-O- β-D-Glucose morin glycosides and its preparation and its application in preparing anti-inflammatory drug |
| CN108395461B (en) * | 2017-02-07 | 2022-01-07 | 浙江康恩贝制药股份有限公司 | 7, 4' -O-beta-D-glucose morin glycoside, preparation thereof and application thereof in preparing anti-inflammatory drugs |
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