CN101490556A - Indicator system for determining analyte concentration - Google Patents

Abstract

The invention provides a method for quantitatively sensing, using an indicator system based on diffusion in space and time of a reaction front, for determining and reporting the prevailing concentration or exposure history of an analyte in food, beverage, and pharmaceutical monitoring for the state of quality, for ripeness indication in fruit, for monitoring environments for concentrations of sanitisers, pollutants and nutrients, for monitoring the residual life of filters, and for monitoring stream flows.

Description

The indication mechanism that is used for the determination and analysis substrate concentration

Technical field

The present invention relates generally to that the sensing analyte concentration changes or the equipment and the method for analyte contact history (exposurehistory), described analyte participates in that food and drink quality, medicine are become sour (spoilage), influence the chemical reaction of quality control in personal protection and environment globality (integrity) field.

Background technology

Have the some gas detection technologies that have been integrated in the electronic installation, they utilize the colored indicator that combines with luminous, fluorescence, reflection technology usually.These matching requirements those of skill in the art's manual operation, calibration and interpretation.The patent example that comprises these devices comprises GB2102947, US5094955, WO0077242, W09627796, the US6908746 that can be used for detecting in food and the blood from the product that becomes sour of bacterium, and US2890177, the US3068073, US3111610, the US3754867 that can be described as detector.

The range estimation reading is used for by Draeger

Interpretation numerical value in the sample hose of making, the technician is used from itself and aspiration pump one to extract gaseous sample and make and places the colored indicator of sample hose to contact target molecule, to obtain visual observation by the coloured band that moves.US 5,653, disclose similar techniques in 941, wherein the gas that becomes sour that extracts in the food containers is manually taken a sample, and according to reporting whether reach predetermined threshold by/failure (PASS/FAIL) test.

If such technology can be integrated into the passive type indication mechanism, promptly do not need the people to get involved, operation can then can be a technical contribution of great use by the system of the numerical value of amateur reader (and being not only the technician) interpretation to measure contact and report under Specialty Design.Passive indicating equipment like this can have some commercial Application, for example is used for food quality (microbial spoilage), is used for the residual life of the contacting of fruit surface, package integrity properties (comprising damaged checking (tamperevidencing)), people and toxic gas, gas mask cartridge filter, the sample kit of expiration, the evaporation-indicator that condenses, blood and urine urea from patient's lung as the freshness indicator.

Other indicator are with similar system simulation true environment.The time-temperature indicator of report that wherein classical is and reactant thermo-contact, described reactant with had similar energy of activation and rate constant by hot system for modeling, and the correlativity that draws provides the inference to true system condition (Riva, M, 1997).

Other indicator are with similar system simulation true environment.The time-temperature indicator of report that wherein classical is and the thermo-contact of reactant, described reactant with had similar energy of activation and rate constant by hot system for modeling, and the correlativity that draws provides the inference to true system condition.Developed the indicator that upgrades, its metering contacts with analyte, and described contact is the immediate cause that causes environmental change.Yet this metering only limits to reach threshold value, thus information (communication) only limit to ON/OFF (ON/OF) or by/failure reading.Such indicator is used to monitor the quality of meat and fish by Food Quality International commercialization, and is used to monitor the freshness of fruit by the Ripesense commercialization.The limitation of these equipment is to rely on the change of observer's visible color spectrum, determines terminal point with reference to colorimetric scale.With regard to interpretation, can't obtain numerical measure with these equipment, to wait until the observer and judge that color spectrum decides, its resolution and accuracy are problematic.

Yet; also do not invent at present the claimed application that comprises following measuring equipment; described equipment passes through engineering structure; use the extraction effect to make the target molecule in the chemical reaction that causes the quality change or change relevant label with environmental integrity and initiatively spread, the direction of described engineering structure makes the quality in described mobile leading edge and the institute's research environment change synchronously.The present invention uses this mobile reaction leading edge to create sensor in device, and described measurement device is also reported the present level or the contact history of target molecule (analyte).

The reading that new equipment of the present invention provides produces point along continuous number scale (not having the upper limit), has therefore satisfied the requirement to hard data (hard data) in quality assurance of current hospitality industry.

Although the present level of analyte provides the information about analyte concentration acceptability in the environment, the contact of the accumulative total of report is intended to the addition accumulation from each time point and analyte reacted during the deployed with devices.

Such device disclosed herein can be used for containing target molecule true environment any sealing or part zone restriction or steady state conditions in, or be used for flowing into or flowing out the gas or the fluid sample stream of this environment, target molecule is by described sample flow.Typical purpose environment of the present invention comprises the biology in food or the biological products become sour reactant or product, environmental contaminants, or is used for air or water are carried out the processing product of sanitized (sanitisation) or the integrality of pesticide and packing air seal.

Summary of the invention

Therefore, an overall purpose of the present invention is contacting or discharging to provide and seal or the chemicals contact history of partially enclosed true environment by report and the target molecule of environmental correclation.

Therefore, in one aspect in, the present invention relates to by the method that contact or discharge the chemicals contact history of the true environment of monitoring sealing of report with the target molecule of environmental correclation, it may further comprise the steps:

Monitoring equipment is placed in the zone of sealing true environment, or place the inflow of target molecule process or flow out the sample flow of described environment, wherein said monitoring equipment has permeable matrix, and writes down and the contacting of these target molecules by the diffusion of this matrix by measuring target molecule; In the contact phase and/or contact phase when finishing, the periodic logging target molecule is by the molecular diffusion degree of these matrix then;

Thereby provide contact and/or the release relevant contact history of this environment with target molecule.

Target molecule can be the molecules of interest of quality management, and can comprise: biology becomes sour reactant or product, pollutant or processing air or processing water to improve the sanitized chemicals of quality.Described purpose target molecule can become sour with food, biological products become sour, microorganism and chemical degradation, personal protection facility, environmental protection are used relevant with other environmental monitorings.

In the time of suitably, described monitoring equipment can penetrating matrix have the chemical indicator that one or more are placed in one, the diffusion of described indicator indication target molecule in matrix.

In the time of suitably, target molecule is induced chemical conversion in matrix, thus the existence of target molecule in the indication matrix.Described chemical conversion can be a redox reaction, maybe can be for example to change the ionization reaction of inducing by pH.Therefore chemical indicator can be the pH indicator.

Can change can penetrating matrix chemico-physical properties (as density and factor of porosity, and/or the aperture of matrix inlet), to improve or to reduce the rate of diffusion of target molecule by matrix.

In the time of suitably, measure the degree that target molecule diffuses through matrix by the reaction of target molecule and chemical indicator.

In some embodiments, the concentration of diffusion report target molecule in linear coloured band that moves or the mobile continuous scale that colour circle is arranged.

In the time of suitably, described monitoring equipment comprises chamber, and its mesostroma places this chamber, and described chamber is arranged to guarantee the change rate of color with respect to continuous scale middle distance synchronously by the diffusion of target molecule in reaction time of guaranteeing to move the leading edge place and the matrix.

Described monitoring equipment can be reported the present level of target molecule or to the accumulation of target molecule contact, or during as integrated equipment its can report present level and contact history the two.

Described monitoring equipment can comprise the reaction leading edge, and the diffusion of target molecule matches in the matrix of described reaction leading edge and indicating equipment.

Described indicating equipment can be constrained to the indicator reaction leading edge along continuous scale by indicating medium to place the pipe of narrow and elongation and advance, carrying out along the plane along the paired observer of the diffusion restriction that indicator carries out.

Monitoring equipment can be set to have the thin layer dish on surface, impermeable upper and lower by the matrix of two dimensional form or have the dish of variable thickness and the indicator reaction leading edge is constrained to along continuous scale advance, so that the paired observer of diffusion restriction is carried out from the external margin to the inside center, perhaps from the center to the external margin, carry out.

In the time of suitably, matrix is set to two dimensional form, as triangle, perhaps is arranged to three dimensional form, as wedge, circular cone or cone form, or other forms of other tapered forms or variable thickness.

Described monitoring equipment can be fabricated in the following way along the non-linear scale that increases progressively and further spread: the length of property band (thermometer form for example of the present invention) along the line changes the stromal thickness that comprises indicator, thereby produces wedge; Perhaps improve thickness, thereby produce semisphere or semioval shape (hemiovular) shape along the radian that is present in ring in the plate-like form of the present invention (plate-like form for example of the present invention).By inlet end being made tapered end, the diffusion of advancing is along with the increase of migration distance becomes more non-linear.Perhaps, can make diffusion linear more to thin end diffusion by the thick end of slave unit.

Can the disperse concentration of report target molecule in the scale of described monitoring equipment, this is by coloured trace of sheltering being set in the position on the coloured band that moves, arriving this position thereby observe band by the color change of position; Perhaps the color of band itself has been sheltered manifesting of following trace, and the shelter band of the migration of advancing by before of coloured band loses color and manifest following information and remind the observer to reach new exposure level.

Described monitoring equipment can be reported with the accumulation of target molecule (as carbon dioxide) by the reactant that use to produce semi-stability reaction product (reversible when 50 to 80 ℃ of mild heat) or stopping reaction product (only reversible under oven temperature) in matrix and contact.

In the time of suitably, described monitoring equipment is reported (comprising buffering agent) present level of target molecule by employed reactant in the matrix, described reactant produces unsettled reaction product at ambient temperature, makes that reaction is instant reversible, thereby produces the report to the analyte present level.

Described monitoring equipment can be by range estimation color moving or separate in the space, report present level or accumulation contact with readable scale (may be to measure) or by means of device (measuring colour developing) with wavelength or frequency, reflection, luminous or fluorescence or other radiotechnologies (as the barcode scanner in supermarket) as catoptrical quantity in the instrument visual field or as chromatogram or colourity (colourintensity).

Described monitoring equipment can be reported present level or accumulation contact by the change of electric signal, and described electric signal is presented on the digital indicator, or is forwarded to Consultation Center by radiotechnology, and may relay to the world by the Internet or satellite communication.

Described monitoring equipment comprises the colorant that contains indicator matrix, or it can use coloured masking layer or background layer to change matrix color or sharpness that the observer sees or that see with the reading that electronic scanning device obtains.

With regard to monitoring equipment, at different audients' AC mode only can change over can by at the coding AC system of acceptor crowd interpretation, with the information of passing on this equipment to contact with target molecule.

Can following calibration monitoring equipment: select to be used to the suitable chemical reagent of indicating concrete target molecule to exist, the concentration of reagent; Perhaps change the rate of diffusion that enters in the indication medium by the permeability that changes matrix.

The microballoon that can penetrating matrix can in pipe, be arranged in linear configuration of monitoring equipment, thus set up tortuosity to a certain degree and set up diffusion slowly by this, advance with certain rate of diffusion with the reaction time of guaranteeing leading edge, and the calibration migration rate.

Described monitoring equipment can contact by indicator is measured accumulation with the extraction reagent mix.

In some embodiments, described monitoring equipment can be equipped with adhesive label or mark, described label or tag and the packing or the container thermo-contact that contain food or biological products.

In the time of suitably, described monitoring equipment can be arranged for the autonomous device that inserts in the packing, be used to be arranged on adhesive label on the packing inwall or trace, with the laminate of anti-solvent material protection or be arranged on the permeable outer wall of packing.

The protective filtering layer can place on the monitoring equipment or with it and adjoin, thereby extracts non-target molecule from measured environment, and the selectivity to target molecule is provided in measurement, and makes that monitoring equipment is to prevent solvent.

Preferably, described monitoring equipment is used for food inspection and environmental quality is used, and the application of monitoring culture of microorganism growth.

Description of drawings

To be basic description the present invention with the non-limiting example shown in scheming now:

Fig. 1: showed indicator, wherein the indicator gel is linear places and is covered by barrier layer, to guarantee the one dimension diffusion;

Fig. 2: the section of having showed linear indicator equipment;

Fig. 3: the indicating equipment of having showed dipstick (dip-stick) device form that is used for immersing liquid;

Fig. 4: showed from the two dimensional surface diffusion of film edge towards the center;

Fig. 5: the vertical view of having showed the disc type indicator of using planar migration in operation;

Fig. 6 has showed the indicating equipment of the form that is tapered, and as wedge, cone, circular cone or other tapered shapes, thereby color change can advance to thick bottom from apicule portion along with the contact that increases gradually;

Fig. 7 has showed the mobile coloured band of migration from left to right;

Fig. 8 has showed the monitoring equipment that is applied to fruit;

Fig. 9 has showed the monitoring equipment that inserts soil; With

Figure 10 has showed the monitoring equipment in the motor vehicles tail gas of packing into.

Detailed Description Of The Invention

May carry out two types measurement in the present invention: present level contacts with accumulation.The former measures the analyte level of record when measuring, and the latter measures the contact element of accumulating with accumulate mode and reports contact history.Under two kinds of contact situations, all can be according to moving the continuous scale of band or discrete scale by the reaction leading edge and having graduated scale to measure and report.Reading can be observation or electronic type.Observation can be used as estimates reading for the non-technical personnel use, and perhaps conduct is used radiowave or passed through the technician use of the electronical reading of other electromagnetic means forwardings for skilled operative installations, and reports to remote control center.

Food contacts hot a period of time and when it become sour biological pollution forfeiture quality at it with biopreparate during distributing.The bacterium and the fungus metabolite that can be used to self destruction food are measured quality forfeiture and remaining quality.Exemplary analyte comprises carbon dioxide, sulfuretted hydrogen, sulphuric dioxide, hydrogen and ammonia, acetate and lactic acid, ketone and aldehyde.Can measure food such as meat and the fish chemical degradation under refrigerated storage by the amine that degrade proteins forms.Can measure the formation of bitter taste product limonin in the orange juice of degraded similarly.The forfeiture of quality also can go into and consume to measure by the oxygen flow in the food prepared therefrom in the packaged food, and reduces by the oxygen concentration that is caused by the caused anaerobic of the plant tissue of the breathing under the temperature that is kept at the design margin that is higher than packaging for foodstuff (anaerobis) in the packaging product and to measure.

The decomposition product of respiration, become sour activity and chemical degradation is acid, alkali or redox product normally, and reactant generally comprises oxygen.The utilization of monitoring formation of decomposition product or reaction product can the indicator organism chemistry and the process of chemical process.

Can use pH or oxidation/reduction indicator to monitor becoming sour or experience becoming sour of avtive spot downstream in the gas of dispersion of environment change or the flow of liquid or upstream in the range of packages.Such indicator can place in pack environment, other limited spaces or the sample flow near the production site, with the monitoring exposure level.

Indicator can react with the evolution product (evolution product) of acid or alkali, and the progress of using the pH indicator to measure drop reaction with dynamic contact by the formation of puting together acid/alkali when having or do not have the pH buffering agent.Similarly, use redox reaction, indicator can be used for measuring analyte (as oxygen) the contact progress in time to concentration change.Can use tolerance concentrated and that the conduct of evaporation indicator enters moisture in the packaging for foodstuff similarly.

Numerous food product (for example breast) is safe when hanging down bacterial population.What the consuming public was concerned about is quality, and acceptable limit may be different between the individual consumer.The thermo-contact meeting of accumulation allows the population development of becoming sour biological.Breast and similar products like a bit are still before vendible up to certain, only report the bacterium existence or do not exist almost not to be worth.In these cases, the carbon dioxide of accumulating develops or other products that become sour (as the acid of becoming sour) measure population and metabolism is valuable by measuring.The problem of prior art (indication membrane change color) is only to report to have reached certain threshold value, or depends on the device reading of colourity.Improvement of the present invention is the reading of report transverse dispersion (lateralspread), along with contact increases gradually and enlarges, as the colour band thermometer of routine.

Fresh product such as fowl ovum, fruit, vegetables and flower lead breathing with temperature with Arrhenius, and its respiration can be changed by the atmosphere of multiple oxygen and carbon dioxide.The oxygen and carbon dioxide present level of being measured at epidermal surface by the inside paster on fruit paster (fruit-sticker) or the package wall has reflected the current environment condition of temperature and atmosphere, no matter this condition to the results of product after the life-span be favourable or disadvantageous.Similarly, paster can be placed on the outside surface of fowl chorion, with the metering ovum breathe, bacterium one or both in the product that become sour in the shell.

For fresh product, can use the accumulation of a certain amount of cell to breathe and measure freshness, as the multiple plant organ that has separated " breathing the life-span (respiration-life) " (Brash etc. 1995, Bower, J.H.2001).

The current ethylene levels with accumulation can indicate beginning ripe in the respiratory climacteric phase fruit (climacteric fruit), or the ripe stage (this moment, fruit such as pears, junket pears and Kiwi berry were to be suitable for most eating) of indication, do not push check and damage fruit and do not need to use to point.

There is permeable overlayer in the epidermis of product organ.Oxygen in the plant, carbon dioxide, ethene and alcohol penetrate these surfaces, and the possibility of measuring equilibrium level with the present invention is provided.

From the cell of product, separate out and see through carbon dioxide, ethene and other gas (as ethanol) that cuticular cellulose enters the surface and can be extracted by indicating equipment of the present invention and into be contained in product in covering paster on one's body by diffusion, or pass be used for that the trade product uses can penetrating packing wall enter and be contained in the outer paster of packing.Perhaps, one deck that this equipment can be used as in the wrappage is integrated into, or the waterproof in being configured to pack and the autonomous device of leakproof, or is positioned at the opaque package outer that has connecting pipe.

For the situation of food, health product and other perishable farm products or medical sample in other not penetrating containers (as Clear glass bottles and jars), stick anti-solvent label at inwall and make it possible to achieve metering and function of reporting.When using opaque container, can in the pipe of for example tygon or other polymkeric substance, sting needle outlet, can labeling apparatus be used as the sealing paster with repairing the identical mode of cycle tyre middle punch then.

Perhaps, can use bayonet socket to settle metering outfit, described bayonet socket is installed by the pin hole that stings out in package wall, and links to each other with the pipe of the metering bag of packing into.These methods make it possible to monitor in opaque pipe and container.

The definition of " packing " can extend to several external packings than inner wrapping, and can comprise bulk container, comprises cask.Obtainable tolerance comprises current breathing and maturity state, or the breathing of product or maturity history.

In order during distributing, to carry out effective qualitative control, to follow the trail of in (audit trail) at the modern auditing of transparency of seeking to sell and responsibility, expectation report product is going bad up to final exhaustion point gradually from results or food processing.Therefore, the expectation metering system shows the expired degree (degree of expiry) when this life of product is in each side's hand of transportation and storage.

The quality of fresh product is along with delay of handling during distributing and inferior optimum temperature (sub-optimal temperature) management and go bad the forfeiture freshness.Freshness in the trade when fruit is plucked or beginning the highest when ripe.Although other food antiseptic methods by freezing, tinning or processed food and beverage provide preservation, become sour biological pollution and chemical degradation finally can limit storage life and shelf-life.Monitoring freshness state is a problem to be solved in the present invention.

The quality of food goes bad along with the thermo-contact during distributing, because contaminating microorganisms growth and breeding.The metabolism of microorganism is the principal element of food degraded, and is regulated by the factor that comprises temperature, oxygen and gas concentration lwevel, somatomedin, water activity (water activity), growth inhibitor and antiseptic.Therefore, temperature-time-giver does not reflect the total environment factor (especially for the preparation of mixed food) of regulating growth of microorganism, and therefore, the change in the monitoring real system is more more accurate for quality control than the prediction in the simulation system.

Distribution chain generally includes the delay between multi-party cooperative, thermo-contact and results or food processing and the household consumption.Freshness is the highest when the good food of manufacturing is packaged.The link that modern distribution system relates to from distribution chain is passed to next link, generally includes for the manufacturer's stock for the processed food and results-cooling for fresh product and the packing-storage in food processing factory (packing-house).Distribution generally includes highway, railway, sea-freight and air transport then, then is that wholesale stock-retail inventory-retail display-consumer purchase-consumer preserves.

In the distribution of whole market chain, all the quality of product and food to be paid close attention to very much in many ways, this should advantageously be reported on the surface of individual fruit or packaging for foodstuff by information equipment (communication device).This information can provide marketing information (marketing intelligence), and may wish that for the purpose of qualitative control acquisition is at the early warning of food quality before this information is delivered to consumer-client once side (for example retailer).This can make the retailer to get involved and product is withdrawn from sale, or to give a discount and handle in order to sell fast.

For protect they on market in the reputation aspect the high-quality product; the retailer prefer to the restriction client obtainable information about food quality; but guarantee the security of its food behind the scenes with the inner management system, do not cause the worry of client to the risk of the imagination or impression.Similarly, they can select to refuse wholesale dealer's delivery when being unsuitable for buying.For the signal that makes the quality state passes at multiple audient, encoded signals is used in expectation.

The present invention is associated with the distance of coloured band migration by the color change speed in the indicator and has satisfied this needs.Realizing higher reliability aspect the corrupt biotic population of report and activity in the product cell and the metabolism by this.The invention provides and make about the information pointer of quality state each side to distribution chain, the rotten and dissociate water of described information and the product that becomes sour is gentle/or the consumption of reactant be complementary.These are according to understanding the real compatible of report that needs carry out and marketing and distribution.

An example of such coded message transmission is at first to use the indicator with early stage quality forfeiture coupling changed to carry out detection of electrons, and this is by for example stock clerk or check operating personnel's bar code scan of (point-of-sale) when selling and finish.In the more advanced stage, the visual information transmission can combine with bar code, and if the further client who goes bad after extending to sale of quality during the client operating.Under the situation of family as the product client, food can go bad to higher level when storing from the hot on the way home car in shop neutralizes owing to refrigerator and kitchen in relatively poor temperature control., can exchange better with visual form (as warning symbol and literal) about the warning of food quality to must abandon food the time when rotten serious, can see by interpretation widely with by everyone like this this last side (terminal user) in the distribution.

There is indication mechanism in the prior art, the conditioned disjunction degree of thermo-contact in the incidents such as its deduction freeze thawing.Time-temperature device is placed in and food and biological products (as packed blood) thermo-contact, and has the identical thermal history that is subjected to when product is distributed.The different process of real system that enzyme process that biological chemistry is processed in these equipment or physical diffusion process relate to and simulate, and according to correlationship real system modeling and calibration.

Have such equipment in the prior art, described equipment is breathed microorganism with the growth medium load, thereby produces acid product in response to thermo-contact from breathe, and described microorganism is the yeast of growth when frozen food melts for example.Yet prior art is not used the specific culture kind of studying in real system.Under the situation that newborn and fish are become sour, know special bacterial species recent years---the cryophile that grows under chilling temperature has mainly caused the food in the modern food distribution system to become sour.

The present invention can the process of becoming sour more approaching and that is virtually reality like reality exactly.The available known culture inoculation separate equipment that causes the specific microorganism of becoming sour of becoming sour is as the viscosity silver on the food containers outer wall.Microorganism can mix with somatomedin in chamber (it has out the opening to sensor), described somatomedin comprises the preparation sample approaching with real food (for example with freeze-drying or vacuum-packed form), have the microbial contamination level that reflects true system, it may dewater, and food when beginning distribution by hydration, start working from vacuum-packed state ventilation or the room temperature when refrigerated storage temperature is transferred to distribution, so that microorganism can grow and breed.

According to this method, lactic acid loses can be by the colored band order report that moves, described colored band indication comes from the rehydrated psychrophilic bacteria culture sample in dry Ruzhong, it has represented the common level of pollution in the normal process, wherein sample is connected with the viscosity silver by pipeline, and equipment is assemblied in newborn outside of containers, and distribution and at home between the storage life with the thermo-contact of food breast content.

A kind of similar application of the present invention is the forfeiture of package encapsulation in the monitoring vacuum-packed food, oxygen can flow into because if sealing is lost then, and causing the growth of nonactive microorganism (known be aerobic and be classified as harmless), color can change in response to microbial growth and metabolism in the indicator tolerance.In this case, equipment can place packages sealed.

Have the oxygen indicator that is used to report food quality, it makes this indicator contact food packing ambient air when using by being opened in packing and reports ingress of air (21% oxygen) back elapsed time as the contact timer.Therefore, the time of packing after opening can be with to estimate that contact swims in airborne microorganism relevant, because the eliminating effect of package encapsulation is lost.In addition, when the consumer will be packaged in when opening in the air, can carry out rough association at the expection oxidation of food.

Yet, food is quality variation when distributing to the consumer, the expectation of food manufacturer and retail trader is measured and the contacting of varying number oxygen molecule, described oxygen molecule penetrates and is designed to the wrappage that vacuum level maybe can't be carried out gas exchange, infiltrates owing to package encapsulation destroys the hole that causes at storage, transportation and selling period.This can provide the more accurate tolerance to food autoxidation degree during the distribution.In addition, measurement is valuable for the oxygen concentration of the penetrating extra package inside of respiratory products (as the vegetables of Min. processing) for the development of reporting anaerobic (anaerobis), anaerobic not only causes the quick aging of plant tissue, and promotes to threaten the dangerous anaerobion growth of human health.

Adhesive label, transparent wrapper wall composition and the packing embolus of use on can penetrating package wall realizes that such measurement is an one object of the present invention, described embolus for example places the label in the packaging for foodstuff, and its measurement and report are passed barrier film (being fixed in the box in the plastics wine bag in the bag packing wine " bucket " as insertion) or pass the oxygen that bottle seals penetrating.

Package integrity properties is important for food quality and security, and bacterial cell and fungal spore can enter by the breach on the package wall.When being damaged, packaging for foodstuff loses its sealing.Manufacturing defect also may fail to produce effective seal.Many packings are designed to the sealing that prevents that bacterial cell enters in the air, but whether bubble-tight, for example some plastics breast containers.In these cases, the effectiveness of the indicator that becomes sour is limited, unless it can extract the gas of escaping or the liquid product that becomes sour when the product that becomes sour produces.These gases or liquid (no matter being acid or alkali or oxidation/reduction reaction product in reaction) should react in semistable reaction with indicator, otherwise reporting techniques does not have genuine and believable degree.Prior art is only reported the threshold value that has reached acid/alkali or oxidation/reduzate, yet this improvement is extracted and has also been measured the reaction product of emitting in the packing with slight leakage or design hole, described in other cases product can from packing, leak and detect less than.

A kind of similar application is the breakage of report packaging product.Preferably before selling, detect the breakage that food, drug products or the like are packed in the electronics mode, and only when scanning system does not detect recent breakage, just report to the consumer with scanning device.Disclose the some indicator that are used to report the forfeiture of pack environment integrality in the prior art, some of them relate to the oxygen and carbon dioxide indicator.Food supplier particularly retailer wishes to realize early intervention under the situation that package integrity properties goes wrong, if but their internal control system is failed this health risk of the then obligated warning public.

For improved commercial Application, the most handy early warning of early detection system (disappearing as bar code) reports to the retailer, then reports to the consumer with printed information or symbol from the senior detection with the higher reaction level of indicator.Early detection can be the realizing than low side of the discrete scale of setting up by measuring system of the present invention, and senior warning is set at higher exposure level; Although the signal code difference, they have reflected the varying level of discrete yardstick.

To the environmental monitoring of air and aquatic target molecule (comprising pollutant) is another kind of the application, and wherein the present invention can be used as passive monitoring equipment and is used to monitor and the contacting of target molecule.

Present level in the environment is significant (especially under the concentration that enough causes warning, when polluting the main cabin of motor vehicles as carbon monoxide emission), because this has the risk of acute poisoning; The accumulation contact can cause that the lower hidden level of slow poisoning also is significant, for example uses the situation of the household heater of no flue burning in school, perhaps the heavy metal ion in the waste water.

Situation for motor vehicle emission, the accumulation contact that is opposite to sampling equipment in the tail gas stream can be reported the automobile of pollution, perhaps measure discharging, thereby only allow suitable vehicle or be in permission contaminant capacity (license-to-pollute quota) to enter the interior zone that pollutes the city with interior vehicle for the permission purpose.

When the output of monitoring chemical process (as the pollution from ventilating opening or pipeline discharge), level can change in time, if concentration in time is variable and accidental, the confidence level that then depends on the sampling of discrete time point can cause inaccurate.The duplicate measurements present level is an effort and expensive to obtain contact history.Continuous Contact may be the more reliable tolerance of chemical product effect in the environment.The present invention of contact indicator is a novelty in these needs are provided.In sample flow (as chimney gas outlet, waste pipe or atmosphere, as using the ozonosphere of measuring the top, land that balloon obtains) sensor of the separation used of medium and long distance makes that a plurality of monitoring stations can monitoring around the clock in the system of robotization, and is similar to data recording.When the monitoring phase finished, the technician can obtain to accumulate the range estimation reading or the RI radio intelligence of contact, and carried out interpretation according to the scale that provides.The manufacturing cost that electronic data recorder is lower makes it possible to realize bigger sampling effect with more monitoring station, and if low cost equipment owing to some disasters are lost, then less to the influence of research budget.

Stifling and sanitized is used the monitoring technology that also can benefit from extensive report analysis thing level.The stifling of the sterilization of water treatment (for example chlorination of potable water, swimming pool or oxidation processes), baby' diaper and room, the packing of product, soil also need be about the information of contact.Dosage is usually by calculating the analyte concentration time of multiply by.Should use the present invention, use the sensing indicating equipment to report current exposure level and contact history valuably by representative sampling point in environment.

Set up the test tube problem of environment above in pack environment, in the building in the scope in room, sample flow, seeing through can be penetrating or the wall of sponge plastics food bag or polluting situation about using in air hole or the pipe and discussing.Having the annex that enters the pipeline in the flora conduit can avoid needs to set up the sampling chamber; for example pipeline can be connected with the equipment that can enter analyte generator (as gas outlet), for example can in protectiveness but permeable pressurized capsule (capsule), place and be used to make liquid mobile through pipeline.This equipment can use with woven hose and other device, and described other devices use in scientific instrument usually, to obtain and the contacting and obtain to take a sample and flow of target molecule.

Passive monitoring equipment of the present invention can be used for monitoring microbial spoilage and the chemical degradation in the perishable farm products (as packaged food).

This equipment can manufacture in the following way contacting of optionally metering and microorganism (described microorganism growing and threatens human health) on packaged food: indicator is directly contacted with food or biological products, or in independent cavity, contact the true environment thermo-contact of described chamber and food or biologics with food or biologics sample; And the known antibodies at target disease biology is combined with indicator, or the indicator that uses known selective acknowledgment to have the composition of antigen susceptibility molecule in some indicator or the manufacturing of the certain enzyme of the bacterium of becoming sour, or use pin selectivity microbiotic, fungicide or other growth inhibitors, its to be not the microbial species of monitoring objective have specific function but to being that the species of monitoring objective are harmless.

This equipment can be used for reporting the oxygen and the moisture of migration feed product packing, and they cause the rotten of food quality.This equipment can be used as the laminate in the package wall, with acting on anti-solvent and the nonwettable equipment that inserts package contents, or as adhesive label that can penetrating wall at this class packing.

This equipment can be used for monitoring the freshness of product (fruit, vegetables, flower arrangement and leaf).This equipment can be reported carbon dioxide, oxygen, ethene and the present level of other purpose gas homeostasiss and the aging of plant tissue, and contact history.Use this information deducibility to go out the current state of homeostasis, aging, freshness or the residual life of maturity state and storage, transportation and product sold.Also can monitor the environmental baseline of oxygen and carbon dioxide in the atmosphere.This equipment can be used as the laminate in the packing of product wall, as be used to insert the anti-solvent of package contents and do not soak into and eat after be safe equipment (because the material of composition is selected), or as adhesive label that can penetrating wall at this class packing.

By combining with the injection device that enters relevant water, nutrient or vegetable food product and enzyme conduit; Or by described equipment is placed on the monitored plant tissue epidermis to extract emergent gas as adhesive patches, this equipment is used in monitoring plant health and homeostasis in the complete plant.

This equipment can be used to monitor sweat in the compost of food processing and manufacturing, wine brewing and organic waste materials and potted plant soil (pottingmixes).Similarly, it can be used for monitoring the biologically active in the soil.

This equipment can be used for monitoring in the atmosphere of packaged food (as grape) or in the fumatorium or place the present level and the contact history of fumigant under the stifling blanket on soil or the timber.

This equipment can be as the monitoring equipment of guaranteeing effective dose when handling water with sanitizer, for example to the water in the swimming pool and the situation of carrying out chlorination and oxidation from the potable water in suspicious source.

This equipment can be used for monitoring the present level and the contact history of pollutant in the air such as carbon dioxide, and carbon dioxide is usually as the indication gas from the dusty gas scope of timber in the buildings (as family and school room) and mineral fuel.Can report the accumulation of undesired gas in relatively limited space (as motor vehicle cabin), described undesired gas is as causing sleepy carbon dioxide.The information that this equipment produces can be supported the decision that need ventilate to compartment that the people is arranged and buildings.

This equipment can be used for monitoring the present level and the contact history of water pollutant (for example from the waste pipe draw-off that enters the water route by pipeline), and rope and floating thing or weight can be housed, thereby is placed on required sampling depth.

For the people who in toxic gas, works, as emergency ward personnel, pesticide user, workman and spray oils lacquerer mine for coal, this equipment can be used for monitoring present level and the contact history in the finite space, and can place the more big room of workplace, or place the cartridge filter of the respirator that the workman wears as personal safety equipment.

This equipment can be used for monitoring air-flow or the current that contain the concentration known molecule by reasoning, and purpose is to produce the indication of contact history, as airborne ambient oxygen (21%) or carbon dioxide (0.04%).Contact model with variable concentration, flow and time can be suitable for calibrating sensors, thereby in time measures the gas or the liquid volume of process sampling spot as flowmeter.

A kind of application of this method is to use above disclosed presumption model to monitor and replace filter plant in air or the current, for example the internal combustion engine of working in the dust environment (as farm tractor) or the Hoover that uses in the cleaned industry at home and the air filter of air-conditioning.Present industrial practice is to change after many hours operating period or the cleaning filter, and this hypothesis rotation speed of the fan is constant.This gage probe can be used at the monitoring of the engine in the work because variable fan speed is taken in contacting of being caused with the air relevant with accidental engine rotation.A kind of relevant application is monitoring and metering and predicts when the water process current sampling spot of certain volume and need clean the swimming pool filter.The improvement of engine life simulation be can be used as the improvement that is better than present engine working time (engine-hours) or Vehicle-Miles of Travel meter reading tolerance measures and uses.The oxygen of accumulation take in or the exhaust (as carbon dioxide) of accumulation thus can represent the life-span more accurately and represent the residual life of engine, and be used to cause the needs of maintenance requirements and engine replacing.

This equipment can be used for monitoring that specific ion (comprises hydrogen (H in water, air, medicine, animal doctor's sample and the water ⁺)) present level and contact history.

Can be by forming indicator by the known moisture absorber and the indicator that condenses, this equipment entered as water translocation pack and the indicator in other spaces that desired conditions keeps dry.

Monitoring equipment comprises the inert carrier medium usually, and described inert carrier medium can contain carbon polymer such as polyvinyl alcohol (PVA) is formed by inertia is water-soluble.In order to ensure the aqueous chemical reaction, carbon polymer can be polyvinyl alcohol (PVA), polyvinyl pyrrolidone or some other water-soluble polymerss, or other transparent or semitransparent wrappage that use in food and the biological products distribution.

Foundation can comprise propylene glycol, tetramethylene glycol, pentamethylene glycol or any glycol or poly-hydroxyl material by the plastifier of the required penetrating rate of mounting medium.

Be used for reporting that by color change acid vapors exists or non-existent exemplary pH indicator can be phenolphthalein, universal indicator or near other indicator that change color the pH8.0-10.0 scope, or any other pH indicator, or the different indicator combinations of widening the color possibility, or the different indicator combinations of widening the color possibility; And can at first be dissolved in alcohol or the suitable polymer solution.

It can be sal tartari, sodium carbonate, lime carbonate or strong cationic other carbonate of organic or inorganic that alkalescence is extracted material, or cationic water soluble hydroxide of other strong organic or inorganics or oxide; Or any basic matterial.Example comprises carbonate, oxyhydroxide, perhaps alkali metal oxide or organic base by force, and it neutralizes in acid vapors.

The acidic extraction material can be acetate, tartrate, citric acid and other weak organic acids.

The pH buffering agent can be based on carbonate or phosphatic buffering agent, and the amino acid of carboxyl-amino reaction takes place, or any buffering agent of anti-pH change.

The reagent that indication ethene exists comprises potassium permanganate (color becomes colourless or brown from purple) and tetrazine derivatives (color becomes colourless from purple).

The reagent that indication oxygen exists comprises the leukomethylene (leucomethylene blue) of the Classical examples that is considered to extract and indicates, and many other leuco dyes.To the colourless thionine dyestuff of leucoMB[] the most similar dyestuff generally is colourless, and is oxidized to blueness, green or purple when having oxygen.Another kind of indicator dye is cherry rubrene (rubrene), and it becomes colorless when having light and oxygen.

Hinder gas transfer enter following indicator barrier film can by thin can penetrating plastic foil such as polyolefin or Polyvinylchloride form.

Waterproof material and prevent reagent from indicating equipment migrate to food allow simultaneously gas (as carbon dioxide) fast penetrating examples of materials comprise silane (as silicone).

The selective permeation of target molecule (as carbon dioxide) can be realized by the mounting medium that applies indicator with coating material (as silicone or tygon).

The example of suitable indicator, polymkeric substance and other suitable reactive chemicals is open in WO9209870, and its disclosure is taken passages as follows.

" big quantitative response is relevant with color change.In every type color change reaction, all have several compounds, and each class all have some compounds that color changes.Be some reaction types and some classes of compounds that can in equipment of the present invention, be used as indicator and activator below.

Color change reaction and indicator are used to detect and monitor organic and inorganic and organometallics.This class color change reaction and compound are enumerated in a large amount of books, summary and publication, comprise below with reference to reaction listed in the document and compound: Justus G.Kirchner, ＂ Detection ofcolourless compounds ＂, Thin Layer Chromatography, John Wiley ﹠amp; Sons, New York, 1976; E, Jungreis and L.Ben.Dor., ＂ Organic Spot Test Analysis ＂, Comprehensive Analytical Chemistry, the 10th volume, 1980; B.S.Furniss, A.J, Hannaford, V, Rogers, P.W.Smith and A.R, Tatchell, Vogel ' s Textbook ofPractical Organic Chemistry, Longman London and New York, 1063-1087 page or leaf, 1986; Nicholas D.Cheronis, Techniques of Organic Chemistry, Microand Semimicrn Methods, Interscience Publishers, Inc.New York, 1954, the 6 volumes, 447-478 page or leaf; Henry Freiser, Treatise on Analytical Chemistry, JohnWiley and Sons, New York-Chinchester-Brisbane-Toronto-Singapore, 1983, the 3 volumes, 397-568 page or leaf; Indicators, E.Bishop (Ed.), Pergamon Press, Oxford, U.K., 1972.These reactions and compound can be used to write down contact history in monitoring equipment.

Oxygenant can with the reduction the dyestuff oxidation and introduce color change.Similarly, reductive agent can and be introduced color change with the reducing dyes of oxidation.For example, ammonium persulfate can be with the empurpled crystal violet of colourless leuco crystal violet (leucocrystal violet) oxidation.Reductive agent (as sodium sulphite) can be reduced into leuco crystal violet with crystal violet.Therefore Oxidizing and Reducing Agents can be used as indicator.Representational common oxygenant (oxidisability reagent) comprising: ammonium persulfate, potassium permanganate, potassium dichromate, potash chlorate, potassium bromate, Potassiumiodate, sodium hypochlorite, nitric acid, chlorine, bromine, iodine, cerous sulfate (IV), iron chloride (III), hydrogen peroxide, manganese dioxide, sodium bismuthate, sodium peroxide and oxygen.Representational common reductive agent comprises: sodium sulphite, natrium arsenicum, sodium thiosulfate, sulphurous acid, sodium thiosulfate, sulfuretted hydrogen, hydrogen iodide, stannous chloride, some metal (for example zinc), hydrogen, ferrous sulphate (II) or any ferrous (II) salt, titanium sulfate (II), tin chloride (II) and oxalic acid.

The Acid-Base reaction is colourless, but can monitor with pH susceptibility dyestuff.For example, bromophenol blue becomes blueness with alkali (as NaOH) when contacting.When the bromophenol blue of blueness contacted as acetate with acid, the series of color change can take place in it, arrived yellow green to green as blueness to green.Therefore, bronsted lowry acids and bases bronsted lowry can combine with pH dependence dyestuff as indication mechanism.It below is the representative example that can be used for detecting the dyestuff of alkali: acid blue 92; Azogeramine, acid red 88, acid red 151, alizarin yellow R, sodium alizarinsulfonate %, acid violet 7, azure A, bright orange, bright green, light blue G, bromcresol purple, bromthymol blue, cresol red, m-cresol purple, o-cresolphthalein ammonia carboxylic complex compound, o-cresolphthalein, turmeric, crystal violet, 1,5-hexichol kappa trap, ethyl red, ethyl violet, fast black K salt (Fast Black K-salt), indicarminum (Indigocarmine), cationic green melachite, peacock blue hydrochloride, the peacock green oxalates, methyl green, methyl violet (alkali), methylthymol blue, murexide, naphtholphthalein, dimethyl diaminophenazine chloride, Nile blue, two alpha-Naphthol base-phenmethylols (alpha-Naphthol-benzein), pyrocatechol violet, 4-phenylazo phenol (4-Phenylazophenol), 1 (2 pyridine radicals-azo)-beta naphthal, 4 (2-pyridylazo) resorcinol sodium salt, quinizarin (auinizarin), that pyridine of quinoline red (Quinalidine Red), thymol blue, Tetrabromophenol Blue, thionin and xylenol orange.

It below is the representative example that can be used for detecting sour dyestuff: acridine orange, the bromcresol green sodium salt, the bromcresol purple sodium salt, the bromophenol blue sodium salt, Congo red, cresol red, directly freeze Huang (Chrysophenine), chlorophenol red, 2,6-dichloroindophenol sodium salt, eosin, Erythrosin B, peacock green alkali, the peacock green hydrochloride, methylviolet base, murexide, between aniline yellow, methyl orange, methylviolet base, murexide, between aniline yellow, methyl orange, the methyl red sodium salt, naphthalene chrome green (Naphtho-chrome green), naphthol green alkali, phenol red, 4-phenylazo-aniline, rose-red, resazurin (Resazurin) and 2,2 ' 4,4 ', 4 ＂-pentamethoxyl triphenylcarbinol.

Organic chemicals can detect by the existence of its functional group.It is known that organo-functional group detects, and has developed and be used to detect most of organo-functional group, and can be used as the basis of indicator-activator combination.For example, cerous nitrate with have fatty alcohol and (take place during OH) as the organic compound reaction of functional group yellow to red color change.Organic compound with one or more following representative functional groups can be used as activator in equipment: alcohol, aldehyde, allyl compound, acid amides, amidoamino acid (aminesl amino acids), acid anhydrides, azo-compound, carbonyls, carboxylic acid, ester, ethoxy, hydrazine, hydroxamic acid (hydroxamic acid) diimide, ketone, nitrate, nitro compound, oxime, phenol, phenolic ester, sulfinic acid, sulfanilamide (SN), sulfone, sulfonic acid and mercaptan.Above there are thousands of kinds of compounds under listed every kind of functional group's kind.For example, below be the amino acid whose exemplary lists that can in equipment, be used as activator: alanine, arginine, aspartic acid, halfcystine, glutamic acid, glycocoll, histidine, oxylysine, lysine, methionine, phenylalanine, serine, tryptophane, tyrosine, alpha-Aminoadipic acid, alpha, gamma-DAB, ornithine and methyl amimoacetic acid.The all colourless purple-bluish violet that becomes when all a-amino acids and ninhydrin reaction.In addition, below be some specific amino acid tests: 1) the aromatic ring coupling of diazo salt and tyrosine and histidine residues produces coloured compound.2) condensation under acid condition of the indole ring of dimethylamino benzaldehyde and tryptophane forms colored compound.3) reaction of alpha-Naphthol and hypochlorite and guanidine functional group (arginine) obtains red product.Below be the exemplary lists that can be used as the a-amino acid of solid amine: lysine, oxylysine, alpha, gamma-DAB and ornithine.Below be some other the selected example that the organic compound of change color takes place in the presence of functional group's test agent: the primary, the second month in a season and uncle's fat and fragrant amido can be with 2, the detection of 4-dinitrofluorobenzene.Observed color change is to yellowish-brown from colourless.Furan Aldehydes reaction in fatty amine, aromatic primary amine, aromatic secondary amine and amino acid and the glacial acetic acid obtains the schiff bases (Schiffbase) of purple.Multiple triphenhlmethane dye and sulphurous acid reaction produce colourless leucosulfonic acid derivant.When making the reaction of this derivant and fatty aldehyde or aromatic aldehyde, obtain coloured product.Decoloured by sulphite when magenta contacts with aromatic aldehyde with fatty aldehyde, form bluish violet (violet blue).Decolouring when peacock green contacts with aromatic aldehyde with fatty aldehyde forms green.

Big quantitative response is accompanied by fluorescence and changes, rather than the color change in the visible region.Some fluorescence indicators are known (Vogel ' s Textbook of Quantitative Inorganic Analysis, the 4th edition, Longman, 776 pages).

This equipment and change thereof are not limited to the combination of chemical indicator, and described combination is relevant with the chemical reaction that produces color change.Also comprise following any two or more compounds, remarkable or measurable physics can take place and change in described compound, and described change can be monitored by suitable analytical instrument.Such change comprises grain size, transparency, conductivity, magnetic and solubleness.For example, can monitor the change of conductivity by electrometer.”(WO9209870)

Might use that the present invention is passive to experience tolerance that indicator obtains and the scope of information combination is explained in table 1.

Table 1-range or metering possibility

Using the appearance or the disappearance (for example can obtain by the phenolphthalein composition in the indicator) of color is a kind of preferable methods, because reaction does not have wavelength shift when carrying out, change but absorbance takes place, and absorbance changes in the range estimation detection and provide higher accuracy in the interpretation of metering progress.

In table 1, as can be seen, can use robotization of the present invention and passive device to monitor and contact with the present level of report analysis thing or with the accumulation of analyte.Also two kinds of application combination might be advanced in a kind of equipment, thereby be reported present level and accumulation level simultaneously.

In the present invention, the current concentration of measuring soda acid or redox reaction thing or product in six kinds of modes contacts with accumulation.

In first kind of mode, the concentration of the reaction product that forms in colourity and the sensing-indicator is relevant, use the saturation degree of colourity to come stoichiometric level according to Beer law.This can utilize naked eyes to distinguish along with analyte diffuses into the ability that colourity is developed and reacted in sensing-indicator gradually as the migration leading edge thereupon.The colourity that obtains is directly proportional with the concentration of current molecule, or is directly proportional with the reaction product amount under the situation of accumulation contact, therefore is directly proportional with contact history.

This form of the present invention is preferably in reacting leading edge and diffuses on the same plane of deep layer reagent more and watch, and can comprise and can come the device of measure signal intensity or wavelength or frequency by colorimetric, reflection, luminous or fluorescence.

In the second way, with the level of analyte with relevant along the colored rate travel and/or the colored displacement of reaction leading edge, use reaction rate to come stoichiometric level according to Fick law, described reaction leading edge is by in sensing-indicating equipment the ad hoc structure of diffusion restriction in circuit or plane being determined.This form of the present invention is preferably in the plane vertical with reacting the leading edge migration and watches.

For second kind of form is described, sealed by barrier film if detect the surface, upper and lower of membrane matrix, expose its edge, then active agent enters the edge that can be limited in laminate.Color fringe begins to move from edge or the zone that exposes, according to Fick law, and square being directly proportional of the distance of color migration and time.Therefore when the target molecule of contact constant density,, then in two days, can show 1.4mm if in one day, show the colour migration of 1mm.Identical indication film only need be calibrated at concrete application and get final product.

Perhaps all edges of the thin dish of above-mentioned sensing-indicator can be sealed, but sealing the margin heart perforation therein then makes color change therefrom mind-set edge migration, thereby obtain the sensing-indicator of second kind of form.Can and make one section contact analysis thing create the similar effect of linear color migration by the linear silver sealing that will extend.This second kind of form of the present invention can be estimated reading along continuous scale metering by the people, and described people for example monitors the operator of perishable items without the complexity training of meticulous instrument during storage, transportation, distribution, sale and consumption.

In the third form of the present invention, the indication that the electricity that can detect sensor of the present invention is led, potential difference (PD) or resistance change.When with separate power source such as battery or solar cell for supplying power, can transmit electronical reading by the radio frequency evaluation apparatus, described equipment can be used as the printed circuit in the packaging for foodstuff at present.This signal can transfer to remote centre by the radio signal transponder.There is the technology that can be used for this class transmission in the industry.Identify (RFID) label comprising the radio frequency on the packing during being used to distribute, and based on the GPRS (GPRS) of-GSM; Morris etc. (2003) provide the description of container sensor, and described sensor reads temperature and it is reported to base station unit on the ship, connect via satellite to propagate and are watching on the Internet for interested group.Although report is by the temperature of thermistor (temperature) sensor measurement usually for these, the reaction leading edge sensor of migration of the present invention can combine with this class circuit similarly.

The fumigant of space such as packaging for foodstuff, airflow flowing or current, room air, the water volume that is used for handling or product carton is limited in to a certain degree and establishes some concentration of target molecules in these environment.The present invention is used to report that the application of current state relates generally to report the concentration of target molecules that rises or descend in this class confined spaces.

In patent EP0627363 with a large amount of individual sensor the carbon dioxide level in the report fresh products is packed on discrete scale.On the contrary, the objective of the invention is to make a sensor to produce a plurality of readings.

Can be by the instrument of using reversible reaction (for example with the calibration reagent mix in buffering agent and indicator and the indication medium) to produce target molecule present level in the report environment.

In the manufacturing of mobile response leading edge of the present invention, obtain quick response to environment change by guaranteeing the height permeability that target molecule in the equipment spreads forward or backward along post or plane and experiencing the chemical reaction of mobile equilibrium in the nutrient culture media, described post or plane are post or the planes that reactant is transfused to or product therefrom discharges.Like this, the quick adjustment of concentration of target molecules subtle change has reached new level in the response external environment, and notifies at once.Can be by using the capillary environment and obtaining this effect to produce tortuosity with the limited filling pipe of material.

Can realize indicating the high-permeability in the medium like this: by selecting the indication permeable material of composition and the porous microsphere by in the scope of extension tubing, using high volume mass ratio as the indication medium; Or use the permeability of known production enhancing in crystallization, plasticization, perforation, polymkeric substance expansion or the polymkeric substance manufacturing industry or other means manufacturings of factor of porosity to indicate media.

Strengthen Equipment Inspection and when small pH changes, in the first method to analyte sensitivity, can use the pH buffering agent.Buffering agent can have the pK value approaching with measured typical environment pK scope ideally, and produces remarkable color change in response to the atomic little change of analyte.To show in order measuring, can to realize the sensitivity that strengthens as buffering agent by using amino acid or borate with carbon dioxide.Carbon amino (carboamino) reaction is regulated in the combination of available amino end acid reaction such as lysine or glycocoll (using or do not use borate).It is desirable to, the pH buffering agent should have the pK value approaching with measured typical environment pK scope, and produces significant color change in response to the minimum change of hydrogen concentration.Available for example oxygen meter or other purpose gas or liquid use similar method to measure the minor alteration of the state of oxidation.

Second method uses the extraction of indicator to be used for strengthening the sensitivity of metering outfit.When measuring the purpose chemical ion of low present level, based on the sensor of reversible reaction reply may be a little less than because beyond this low-level range of sensitivity that is in device.By low-level target molecule being extracted in the sensor, can in the trend of color change, show detectable reading, described extraction is accumulated molecule in the mode that adds up.

The form of the present invention of report accumulation contact can be used under the normal operating temperature semi-stability relatively or stable reagent is produced.Can form the still reagent of meeting reverse in about 60-80 ℃ temperature range of semi-stability reaction product if the operating temperature range that is chosen in about 0-60 ℃ is interior, the equipment ability of (recharge) that can obtain to load again then, described reagent can place on the equipment, by mild heat reaction is reversed, with its null value of loading back again.A kind of such reagent that satisfies this requirement is sal tartari, and it is to can be used for measuring the reagent that contacts with acid vapor.

A kind of relevant application can be tackled and be used for extracting the problem that reagent place brings in the alkalescence of making and the storage life measurements contact with acid analyte, can also can be triggered and too early work with the carbon dioxide reaction that exists in the atmosphere because described alkalescence is extracted reagent.During the manufacturing of polymer packaging film, the carbon dioxide that absorbs is discharged in expectation during storage and mild heat (for example film being passed through the baking box environment) processing.Reporting facility can be before packaging product be started working to about 60-80 ℃ by mild heat, thereby the measurement result of report is returned to zero or near zero.

According to principle of the present invention, the reversibility in the time of can realizing that measuring alkali contacts by heating acidic extraction reagent (as acetate and tartrate) is although realize that the temperature range that reverses can be different.

In application, in the manufacturing of load device again, can utilize again load-bearing capacity to measure and the contacting of target molecule.Can it be loaded again by installing heating, heating-up temperature be higher than room temperature, but is lower than the temperature fusing point of institute's materials used in the manufacturing of this device maybe that can produce deleterious effect to the composition of reagent chemistry.

In qualitative control, the consumer wishes to obtain the freshest stock supply, and retail trader wishes to sell the rotten stock that has consumer's acceptable degree on the quality.Therefore, there are some conflicts consumer and supplier between the interests about rotten food or other biological goods freshness.

In the present invention, metering can realize different audients' configuration by making information pointer, during wherein alarm in early days (this moment, exposure level was low) warned in some purpose aspects, and other each side among the another kind of audient receive information when reaction proceeds to late period, this moment, exposure level was higher.

The multiple quantitative model of disclosed color possibility in these following chapters and sections capable of being combined.Information encoded can use specific device (as bar-code scanner) to obtain by food service personnel in the trade or quality control personnel, and described information use to occur or the indicator that disappears and take to lack or add the form of bar code.Also can measure, as colourity or the colo(u)r specification (quantum of colour) that on given space, scans by device.

Electronic information form at concrete audient's classification (as the stock clerk) coding can comprise the bar code reading that obtains by reflection.

Indicator can be mixed so that the color change spectrum of the expansion that can select to be provided, for example from acidity to neutral and to change wide coverage the chemical technology that uses universal indicator of alkaline environment.The color change that obtains can be relevant with the change of exposure level, to obtain scale.

With single color indicator be converted into another kind (for example in following application from pink to black, in the distribution of retail shop, need electronic bar code scanning perishable, the packaged vegetables of mincing and shredding in the described application) a kind of the inventive method be that it is compared at the green transparent layer that places the top or the green background material of below.After the contact, if the color change in the indicator is from pink to colourless, then the effect of green contrast layer is that this change color is become black to green change.

Perhaps, indicator can with do not participate in catalytic colorant and mix, this can change into variation better with regard to the information purpose with change color.

Color change takes place in the existence that the many chemical reactions that cause indicator to change color depend on water; This dependence can relate to the process that the target molecule migration enters indication medium, solubilization and ionization.Therefore, for such application, at selecting effectively to indicate material with the affinity of water, and can with Humectant with experience indicator and mix.Under the operating conditions of humidity, there is a problem, dissipates, lose tolerance because moisture is taken in the leading edge that can induce reaction.Can control this effect by the following method: regulate the concentration of Humectant or set up target molecule by surrounding material (as silicone or tygon, it can limit and enter the water translocation of experiencing indicator) selective permeation, or select to prevent the plastifier (plasticiser) of excessive moisture picked-up at director composition, or use the known multiple salt of in particular range, regulating humidity, or the combination of these methods with indicator.

The present invention might be used for measuring acid or alkaline analyte, or oxidation or regression analysis thing.

Packaged food is to the very sensitive material of ion imbalances, should avoid ion to leak and migrate to via package wall experiencing in the material, otherwise quality and security can be impaired.The selectivity transmission of nonionic molecule can be favourable, and this can realize that described separating layer for example can be made up of the silane that only transmits uncharged molecule (as carbon dioxide) (as silicone) by the separating layer of selectivity transmission.

Another kind method be the polymeric layer selecting to contain microballoon as the stored prod of sensitivity and the film between the sensor, described microballoon has enough narrow diameter, thereby allows less target molecule diffusion, and the bigger non-target molecule of eliminating simultaneously.

Another kind method is to use the removal from produce the sample flow between source and the indicating equipment of filtering layer or scrubber (scrubber) to obscure molecule.Example chemical kind that to be existence obscure, conflict with the bigness scale of the pH or the state of oxidation.An example is: when existing in the volatilization alkali that exists in the degraded fish in the fish packing, measure the carbon dioxide of being discharged by putrefactivebacteria by alkali is mixed with indicator.What the use of filtering layer or scrubber should be removed the protein of degraded and amine from packaging for foodstuff obscures molecule.Perhaps can wash the carbon dioxide (a kind of acid vapor) of discharging, thereby can measure the amine that alkali forms in the reaction more accurately by bacterial metabolism.

Connect for reading is contacted with current concentration or accumulation, the calibration indicator is important to the response of contact.In some commercial Application, short time low concentration contact can need high sensitivity, for example when using indicator to pack the forfeiture of middle integrality by oxygen in the ingress of air or carbon dioxide report.On the contrary, for the long term monitoring of vehicular discharge, higher relatively contact history can be more meaningful.

A kind of method that detects low present level is that small difference is set between indicator and target level, and uses the known buffering agent of only resisting the pH subtle change of scientific circles, so that the little change meeting initiating response in sensor in the chemical equilibrium.

A kind of method of calibration (as the method more extensive than fine setting) is a part of molecule that metering is produced by chemical process between height contact and low contact, rather than all molecules.This can realize experiencing entering of indicator by restriction, and described restricted passage is in the source that produces target molecule and experience and dwindle the access approach that inserts hole or create distortion in the hole between the indicating equipment and realize.

Experience the variable permeability of indicator material and/or surround the variable permeability of material (as barrier film) or absorption equipment on the variable permeability in hole can be used to similarly to calibrate to the replying of contact, other possibility methods that change permeability are to select material, change plastification composite or crystallization degree in the mill.Also can use perforation to improve the surface area that contacts with target molecule with respect to the indicator volume, with the color change in some zone of reinforcement indicator, thus the meticulous interpretation of reaching exposure level.The size in single hole also can be used to calibrate rate of diffusion on the absorption equipment.

In the form of accumulation contact, can be prepared as follows the film of widespread use: make the indicator that thickness is enough to extract extensive quantity (from a small quantity to a large amount of) molecule, thereby each interpretation chart of using can provide the interpretation that is fit to given application.This realizes by the independence of rate of diffusion to concentration gradient.

Another kind of calibration steps is to change reaction rate with damping fluid, and another kind of alternatives is to use the amount of reagent different and the target molecule reaction and indicator and changes reagent/indicator ratio simultaneously, until the balance that reaches expectation and color change takes place.

Also having another kind of calibration steps is thereby that the thickness that changes indicator changes the influence that is reflected in the indicator, this as visible color by visual inspection, or the color of measuring as electronic equipment.Along with the increase of indicator material (no matter being to place pipe or film) thickness, target molecule all causes reacting leading edge towards unreacted coloured migration of agents by the carrying out property migration of successive layers.When observing perpendicular to the film indicator, the thickness of increase can strengthen the sensitivity of contact index gauge as the useful tool of higher contact, because along with contact increases gradually, colourity can be with lower speed forfeiture.When with migration leading edge same plane on when observing, the interpretation as bar tape reading sample is provided in the tubulose position of equipment, provide as conventional thermometer, pipe or band or film are long more, the yardstick that the metering that is provided contacts is big more.

The speed (speed) of reaction leading edge migration can be used as calibration steps and is used for the interpretation purpose that time scale is used.Colourity moves (moving into the darker indicator layer from the observation place of 90 ° of angles) along with leading edge and manifests or the progression rates lost can be used as calibration steps.Perhaps, calibration can derive from the speed of following linear transport: the linear transport of coloured band in the plane identical with the observation place of linear coloured band equipment, or the radial migration under coloured ring apparatus situation.

The migration circle (tolerance of distance) of reaction leading edge also can be used to measure contact and obtain calibration at exposure level.

Extract situation about changing in the receptor for electronic surveying, because the migration of reaction leading edge causes the timely acquisition of electrical characteristics (as electric current or resistance) or the change of losing in the available surrounding environment to calibrate.

These calibration stepss can be used alone or in combination, with metering and the contacting of target molecule.

As mentioned above, existence can be measured two kinds of scale types of accumulation contact indicator: discrete type and continuous type.

A kind of form is target molecule carrying out property contact and form product with reagent reacting in the continuous scale, and to indicate rotten degree, same, the calibration of equipment also is important.

Can come with continuous scale reflection metering in the one dimension diffusion by limited reactions, and can come according to contact described measurement and calibration by the speed of conditioned reaction leading edge according to method disclosed by the invention.A kind of such method with the one dimension diffusion restriction in the pipe (a section is permeable porous) of elongation, as shown in fig. 1.With reference to figure 1 as can be seen the indicator or the indicator film of a printing or the cylinder that contains the full of liquid of indicator gel placed (1) linearly and covered with barrier layer (2), in one dimension, spread guaranteeing.One dimension advances and reflects the contact of being measured by vision, reflection, luminous, fluorescence or by other radiotechnologies.(for example use scissors by removing, or divest from barrier film, or with puncture action, or release bubbling, or by removing any any means known of sealing in the packaging industrial) sealing layer (3) starts working this equipment, and reading is provided and is convenient to interpretation along the advance linearity or the non-linear scale of printing of color (4) neutral line.This figure shows, contact causes coloured change linear travel the 2nd level in 4 levels to the scale.

Fig. 2 shows sectional view, and it shows how spatially to limit diffusion linearly with having the narrow film that surrounds material (being two laminates) under this form, this can use the pipe of having filled the gel indicator to realize similarly.

This equipment can be made into to be used to immerse the dipstick device form of liquid, may be with floating ring with its vertical orientation, to measure the contact from analyte concentration in the solution, as shown in Figure 3.With reference to figure 3 as can be seen, the anti-solvent protection most advanced and sophisticated (1) that is selected for the selective permeation analyte allows analyte to diffuse in the measuring tube, then with the reagent of diffusion and the carrying out property reaction of indicator make coloured leading edge in response to contacting along pipe transfer, use reading scale (2) interpretation of printing, the other end of pipe keeps impermeable sealing (3) simultaneously.

Second method is used in two dimension from the planar diffusion of film edge towards the center, as shown in Figure 4.With reference to figure 4 as can be seen, the dish of indicator trace or film (1) is covered as sandwich by barrier layer (2), thereby diffusion restriction is moved towards the center from the edge in the plane, and advances and reflect the contact of being measured by vision, luminous or fluorescence.

The disk-form of in Fig. 5, having showed using planar migration in operation.With reference to figure 5 as can be seen, linear or nonlinear scale is printed to concentric circles, and color advances on the top seal layer along radius.Color is moved towards the center from the edge in this form, because the edge sealing is destroyed, contact is reacted to centre-driven.According to interpretation scale at concrete commercial Application calibration, the exposure level that the color change representative at each concentric circles place increases progressively.As can be seen, towards the center, color is along with the contact that increases progressively changes to colourless from colour from the edge in Fig. 6.As can be seen, on the scale of trace, the contact of target molecule has been moved a level with color change from external margin towards the center.Perhaps this equipment can be sealed also hole of heart thorn therein, the migration of institute's color change is from the center radiation.

Fig. 6 shows the third form, and it is shaped as tapered wedge, cone, circular cone or other 3D shapes with indicator, thereby color change can advance to thick bottom from thin end along with the contact that increases progressively.With reference to figure 6 as can be seen, contact moves to level 2 on the interpretation scale with the leading edge of color change from the thin end of wedge towards thick bottom.

The progress of coloured band migration can be made into vision, luminous or contact that the reflection of fluorescence ground is measured in the above-mentioned embodiment.

In its path, realize when the position terminad moves that from taking in acceleration or a kind of method of slowing down are at coloured band, take in the hole, provide another input port to analyte in position along the line except providing.In the position along the color transfer circuit, this can realize by the permeability (comprise and pass perforation or the otch that barrier film is made) of the barrier film thickness that reduces circuit section place or layer that reduces barrier film or reduction barrier film.Other method is that the indicator circuit with a plurality of separation is combined into a continuous indicator circuit, and the composition of each section can change about the selection of permeability, reagent metering, buffering agent or buffer level.

In some commercial Application, may need the combination of reading in the continuous and discrete scale.Use is to be used in the food distribution chain indicating food quality because the rotten exposure level that is caused that increases gradually at an example of the coded message of different groups.This can realize so that continuous scale is changed over graduated scale by the coloured band equipment that moves is carried out special change.

Can the coloured band that move be changed the graduated scale of generation by shelter or under indicator strips, print alphanumeric literal or symbol in use on the section of mobile band circuit.Purpose is little by little to shelter or the Show Color change along the route of color diffusion.

For example, the continuous scale of mobile coloured band is manufactured to and produces graduated scale, and fresh level is reflected in the each side in the food distribution.Shown how to realize this purpose in Fig. 7, in this diagram, the coloured band that moves moves from left to right.Use the purple masking layer in the section on this equipment purple band below.If do analogy with subway train, then along with coloured band when circuit moves, it is as being visible along the locational train of subway.

In another kind of situation; if the purple band covers on the following purple trace and the color change linear transport as the top layer color; then Xia Mian purple trace can be disclosed by the reaction leading edge that becomes colorless of process, and following trace becomes visible for the observer.

The continuous scale that this application changes mobile coloured band to be producing graduated scale, and is encoded as each side's report food in food distribution level of becoming sour.As can be seen, the coloured band that moves moves from left to right in Fig. 7.This equipment uses masking layer, in some applications, has layer on the mobile color vitta band, on the coloured trace below indicator strips covers in other are used.Shown that the stage A in the color-bar tape travel arrives E.

Zone 1 is that the observer is sheltered advancing of color change leading edge, takes place under these panels of color change indicator below covering.

In stage A-when producing stock, the migration of reaction leading edge does not cause that distinguishable product is rotten.

In stage B-with product when manufacturer is transported to the wholesale dealer, the migration of reaction leading edge has consumed tolerable indicator and has changed, and causes the color change of zone 2 from pink to transparent.

In stage C-in product wholesale, the migration of reaction leading edge has consumed tolerable indicator and has changed, and causes the color change of zone 3 from pink to transparent.

In stage D-in the retail of product, the migration of reaction leading edge has consumed tolerable indicator and has changed, cause the color change of one of 4 bar codes (zone 4) from pink to transparent, having reflected only can be by the coded message of retail employee interpretation, and the consumer does not know this situation simultaneously.

In stage E-and zone 5 is the indicator masking layers that cover on the information of printing with ink, described ink is identical with the indicator color.When the migration of reaction leading edge, the color of indicator becomes colorless from pink, masking layer disappears, and shows with pink and prints and before be coated over general information colored band (this band before had been pink and had been transparent now) under, is unsuitable for purchase with literal or this product of symbol suggestion consumer.

Fig. 8 shows the paster form of the present invention that places on the fruit outside surface that maturation/aging takes place.In this case, equipment is in central hole and have the breathing of accumulation and the carbon dioxide of emitting with breathing or contact from the accumulation of the ethene of maturation, and the colour circle of expansion shows the tolerance of advancing from level 1 to 3 to this metering outfit from enlarging along with the reaction leading edge.This equipment can place similarly can the inside surface of penetrating packaging for foodstuff on, or on the inside surface of not penetrating packaging for foodstuff (for example packaged food such as meat and fish), or as the packing ring in the newborn container screw top.

Form of the present invention shown in Fig. 9 displayed map 3, it is built into as the equipment of gas level in the monitoring soil, and described gas is for example from the carbon dioxide of geobiont metabolism.In the stage A of Fig. 9, use this equipment, and in stage B, the accumulation carbon dioxide that extracts moves to the level of mating with soil microbial activities groupy phase with colored band along soil surface in the given time from soil.In Fig. 9, gland bonnet 1 be waterproof but penetrating to carbon dioxide, becoming 2 the tube of being labeled as of an angle of 90 degrees with probe portion is graduated, setting up scale, soil profile 3 is represented with tangent plane.

Figure 10 shows the form of the present invention that places motor vehicles tail gas that is built into.In Figure 10, offgas duct 1 is observed from automobile rear, observes from the vehicle that travels behind the pollution vehicle as governability person.Show the new contact arrangement that uses in stage A, color-bar band 2 is half scale in stage B.If the pollution limit of permission is the length of color-bar band among Figure 10, then car owner and the executor of government can reach a conclusion: discharged 50% permitted emissions amount, and inferred present permission residue 50%.

List of references

Bower，J.H.(2001).The?relationship?between?respiration?rate?andstorage?life?of?fresh?produce.PhD?thesis，Centre?of?Horticulture?andPlant?Sciences，University?of?Western?Sydney，Hawkesbury?Campus.

Brash，D.W.，Charles，CM.，Wright，S，and?Byrcroft，B.L.，1995，Shelf?life?of?stored?asparagus?is?strongly?related?to?postharvestrespiratory?activity.Postharvest?Biology?and?Technology?577-81

Morris，S.C.，Jobling，J?J.，Tanner，D.J.and?M，FoTbes-Smith(2003)..Predication?of?Shelf-life?for?Fresh?Produce?TransportedbyRefrigerated?Containers.Acta?Horticulture，604(1)，pp.305-311.

Riva，M.(1997)Time-temperature?indicators，a?review?by?MarcoRiva，Universita?degli?Studi?di?Milano，Italy?1997

Claims (21)

1. the method that is used for quantitative sensing, this method uses the indication mechanism that spreads on room and time based on the reaction leading edge to measure and report the current concentration or the contact history of analyte in food, beverage and the medicine, with the concentration of sanitizer, pollutant and nutrient in monitoring quality state, the maturation of indicating fruit, the monitoring of environmental, the residual life and the monitoring flow of monitoring filter; This method and apparatus comprises:

A. inert carrier medium, its carrying chemical reaction also provides controlled analyte diffusion,

B. have geometrical construction and impermeable barrier material, with the diffusion restriction that analyte entered indication mechanism and be directed to along can be penetrating or the continuum measured of the mounting medium of porous carry out, described continuum is by changing density, factor of porosity, permeability, crystallization or placing the microballoon post and set up

C. load on the reagent in the mounting medium, its with analyte extract into this equipment and and analyte response, thereby in chemically stable, semi-stability or unsettled reaction, provide mensuration,

D. indication mechanism, it reports diffusion analysis thing and the mensuration that the interactional reaction leading edge of reagent place develops terminal point realizing,

E. be used to weigh the quantitative yardstick of contact, this yardstick is used for the vision reading as the scale that shows along the tolerance continuum, or as the galvanochemistry that changes or the signal of electromagnetic property intensity,

F. be used for the window that the reaction leading edge of visual monitoring migration is advanced, the reaction leading edge of described migration is produced along the diffusion of measurable continuum by analyte,

G. analyte is taken in and is absorbed the hole of this monitoring equipment,

H. attachment means is used for equipment is placed with respect to sample flow, or equipment is placed in half boundary of producing the source upper chamber, and described sample flow flows out from the source that produces analyte,

I. be used for the reference yardstick that interpretation reaction leading edge moves, this yardstick is digital calibration (quantitative) in the scale or the ranking (qualitatively) made by scientist or professional quality judging person,

Wherein, analyte spreads with the change at measured analyte in mathematical way and the surrounding environment along measured active of tolerance continuum on room and time and is associated, this is by realizing the described degree that maybe should move coloured leading edge the detection time of moving that moves coloured leading edge and comprising the concentration of the analyte that produces or the contingency table of molecular number compares, thereby establish the seriousness yardstick that analyte quality in the measured environment changes, report that thus the corresponding state of medical material or device is realized.

2. the process of claim 1 wherein that described contingency table relates to oxygen or carbon dioxide enters by the cut in the package encapsulation in the medical science packing of aseptic packaging, to report the forfeiture of package integrity properties by the coloured leading edge that moves.

3. the process of claim 1 wherein that described contingency table relates to the wound dressing carbon dioxide that the sticks progress along with wound healing, with coloured leading edge report wound state by moving.

4. the process of claim 1 wherein that contingency table relates to the concentration of measuring chemical residue in medicine dermal patch or the skin implant by the coloured leading edge that moves.

5. the method for claim 1, wherein said mounting medium by water-soluble contain carbon polymer or any have for any polymkeric substance of the chemico-physical properties of the migration calibration of reaction leading edge forms, described characteristic for example density and factor of porosity, crystallization, plasticization, perforation and polymkeric substance is expanded.

6. the method for claim 1, wherein said mounting medium and barrier material are on every side calibrated the migration of reaction leading edge with multiple geometric configuration, it is as a row microballoon, or have the film band or a dish of potential variable thickness, or enter with diffusion path in tortuosity, or the single aperture of inflow point, or import volume, or the combination of these methods.

7. the process of claim 1 wherein and load in the mounting medium, analyte extracted access arrangement and provide the reagent of measurement result to comprise titration reagent and the redox reagent that is generally used for realizing chemical assay with analyte response; Perhaps as the immune response indicator time, indicator is made up of the required reagent of reaction, comprises thinning agent, conjugate and matrix, and described indicating equipment is by antigen or antibody parcel.

8. the process of claim 1 wherein that described indication mechanism is indicated by the observed mobile coloured band of observation point reports and has realized the advance mensuration of terminal point to reaction leading edge place.

9. the process of claim 1 wherein that indication mechanism is by having been realized the advance mensuration of terminal point to reaction leading edge place by the electrical feature report that this equipment is integrated into the change that circuit produces.

10. the method for claim 1, wherein be used to measure the quantitative yardstick of contact by following means realization: be used to estimate reading by the scale that is used to estimate reading of edge metering continuum distribution, the coloured leading edge placement alphanumeric listing that moves by the edge, or by the galvanochemistry of receiving terminal in the generation circuit or the strength signal of electromagnetic property change.

11. the process of claim 1 wherein that the window that the reaction leading edge that is used to estimate the monitoring migration is advanced realizes by use transparent or semitransparent material on the coloured leading edge that moves.

12. the method for claim 1, wherein be used to make analyte to enter that but the hole of described monitoring device provides by covering the inlet point that contact with the selectivity permeable material with absorbing into, described material by for example remove, excise, tear, bubble explosion or other means can be exposed to this indicating device after removing and sealing to the extraction of analyte molecule on; Or by monitoring equipment being placed the design environment that will test its sealing integrity provide, wherein this equipment brings into operation when the external packing of medical science inclusions is packed and sealed.

13. the method for claim 1, wherein be used for the attachment means that equipment is placed or is placed in half boundary of producing the source upper chamber with respect to the analyte molecule sample flow that is flowed out by the source that produces analyte is comprised: cover described monitoring equipment, make it can be used as autonomous device and be used for inserting in the packing; Be sidelong one and put bonding agent, make it can be used as label or trace fixes, be used to the inwall of packing; With its as viscosity dermal patch or wound dressing and/or place can penetrating packing or the outer wall of viscosity dermal patch or wound dressing on; Or with after the protection of anti-solvent material with this monitoring equipment as the one deck in wrappage, dermal patch or the viscosity wound dressing.

14. the process of claim 1 wherein be used on this device of interpretation or device near the reference scale of reading be alphanumeric or the symbol that is used for carrying out quantitative reading along continuous scale, thereby can be measurable distance to the mensuration of space displacement.

15. the method for claim 1, wherein be used on this device of interpretation or device near the reference scale of reading be made into graduated scale, some position some section on coloured leading edge online or tangent line uses covert, thereby in some sections in path, hide, and appear described mobile coloured leading edge, thereby can be measurable distance to the mensuration of space displacement at other sections.

16. the method for claim 12, wherein use the background colour of sheltering look or mobile colour band below be present in the transparent covering layer when the mobile color with described indicator produces contrast in this position/the scale place produces the traffic lights color change.

17. the method for claim 1, wherein be used on the interpretation device or device near the reference scale of reading arrange in the following manner, the indication dielectric surface that described mode makes coloured leading edge be absorbed first from analyte obtains first reading when beginning spatially to be moved beyond 100 microns.

18. the method for claim 1, wherein can draw a plurality of vision readings from a sensor, the molecular number of analyte concentration or analyte generation is associated with the space displacement of reaction leading edge, and produces regression relation, with the change of evaluation sensor environment from this x-y curve.

19. the method for claim 13 is wherein carried out reading by electronically, it can include but are not limited to light that the described indicator of sensing sends, forward it to massaging device and the data that produce are sent to Consultation Center at a distance.

20. the process of claim 1 wherein and settle one or more equipment to measure contact simultaneously, as the means of reaching coarse adjustment and fine tuning.

21. the invention still further relates to any alternative combinations of the feature of pointing out in this instructions, all equivalents of these features are all thought and are included.

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