CN101474211A - Phellinus linteus extract and preparation method thereof - Google Patents
Phellinus linteus extract and preparation method thereof Download PDFInfo
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- CN101474211A CN101474211A CNA2008102017980A CN200810201798A CN101474211A CN 101474211 A CN101474211 A CN 101474211A CN A2008102017980 A CNA2008102017980 A CN A2008102017980A CN 200810201798 A CN200810201798 A CN 200810201798A CN 101474211 A CN101474211 A CN 101474211A
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Abstract
The invention discloses a phellinus igniarius extract. The extract comprises 20-30% of flavone and 5-10% of polysaccharose. The phellinus igniarius extract can obviously reduce blood sugar and be used for preparing drugs for reducing the blood sugar.
Description
Technical field:
The present invention relates to the medicinal fungi extract, relate in particular to a kind of phellinus linteus extract and preparation method thereof.
Background information:
Phellinus igniarius (L. ex Fr.) Quel. is a kind of rare medicinal fungi, and before 20th century, Phellinus igniarius (L. ex Fr.) Quel. is mainly derived from wild resource, because the wild resource rareness causes the price of sporophore very expensive, per kilogram is more than thousand yuan.In recent years, along with the development of edible and medical fungi cultivation technique, Phellinus igniarius (L. ex Fr.) Quel. has obtained domestication's success, and its later stage development cost is reduced.Because the drug efficacy study of artificial culture Phellinus igniarius (L. ex Fr.) Quel. is not as yet not deeply; relevant deep process technology and Products Development research are few, make the plantation of domestic Phellinus igniarius (L. ex Fr.) Quel. fail to further develop, and the research basis is few; and not deeply, especially domestic tame Phellinus igniarius (L. ex Fr.) Quel. is not lacked deep research comprehensively.Modern pharmacological research shows that Phellinus igniarius (L. ex Fr.) Quel. has effects such as antitumor, antioxidation, anti-hepatic fibrosis, enhance immunity, but is that the product of development of raw materials is still blank at home with the Phellinus igniarius (L. ex Fr.) Quel..
Phellinus igniarius (L. ex Fr.) Quel. to artificial implantation carries out bioactive research, develops corresponding product at its main functional activity composition, is it is carried out the good approach that the degree of depth is utilized, and also is the blank of filling up the rare medicinal fungi Phellinus igniarius (L. ex Fr.) Quel. development and use of China.
Diabetes are a kind of common incretion metabolism diseases, often follow multiple chronic complicating diseases, cause the extensive injuries of many organs, multisystem, have higher disability rate, are the healthy the third-largest diseases of harm humans after cardiovascular and cerebrovascular disease, cancer.About 4,000 ten thousand people of China's diabetics are one of maximum countries of diabetics, and sickness rate are ascendant trend year by year.Though orally-taken blood sugar reducing medicine and insulinize can be controlled hyperglycemia effectively, the prevention of chronic complicating diseases and treatment are still lacked effective measures, take the orally-taken blood sugar reducing medicine continuously and problems such as drug failure, hyperinsulinemia and insulin resistant can occur.
Summary of the invention:
The present invention is based on an amazing discovery, and the extract of Phellinus igniarius (L. ex Fr.) Quel. can reduce mouse blood sugar concentration.
The present invention at first provides a kind of phellinus linteus extract; this extract is that the Phellinus igniarius (L. ex Fr.) Quel. sporophore that adopts the Phellinus igniarius (L. ex Fr.) Quel. strain to carry out cultivation basswood extracts processing and obtains; contain flavone and polysaccharide; weight percent hundred content of flavone are 20-30% in its extract dry powder, weight percent hundred content 5-10% of polysaccharide.
The used Phellinus igniarius (L. ex Fr.) Quel. strain (Phellinus baummii Pilat) of the present invention is numbered: Phellinus baumii Pilat3249 from edible fungi branch center, Shanghai, Chinese microorganism strain preservation center.
Concrete extracting method is:
The selection of Phellinus igniarius (L. ex Fr.) Quel. raw material: select for use Phellinus baummii Pilat strain to carry out the sporophore of cultivation basswood.
With the soak with ethanol of Phellinus igniarius (L. ex Fr.) Quel. sporophore with 75%-90%, get supernatant, concentrate drying obtains extract 1;
After above-mentioned residual residue vapored away ethanol, the water logging bubble was got supernatant, and concentrate drying obtains extract 2;
Extract 1 and extract 2 are mixed, obtain phellinus linteus extract of the present invention.
Preparation method more more specifically is:
The Phellinus igniarius (L. ex Fr.) Quel. sporophore was soaked 24-48 hours under the 75%-90% room temperature, and solid-liquid ratio 1:10-20 (weight kilogram specific volume liter) filters the back and keeps supernatant, residue reuse 80% soak with ethanol 24-48 hours; Merge lixiviating solution twice, in 40 ℃ of following concentrating under reduced pressure, wait to boil off most of ethanol after, add water again and continue concentrating under reduced pressure, 50-55 ℃ of concentration tank temperature, pressure is less than 0.1MPa, be concentrated to proportion 1.050～1.080 (50 ℃ of heat are surveyed), the concentrated solution lyophilization gets phellinus linteus extract 1;
Phellinus igniarius (L. ex Fr.) Quel. sporophore residue after the soak with ethanol extraction is air-dry, fling to ethanol, add water and extracted solid-liquid ratio 1:5-15 (weight kilogram specific volume liter) at 2 o'clock in 90-100 ℃; Filter and collect extracting solution, residue extracting in water more once merges extracted twice liquid, and extracting solution pumps in the concentrating under reduced pressure jar, carry out concentrating under reduced pressure, 65 ℃ of concentration tank temperature, pressure is less than 0.1MPa, be concentrated to proportion 1.050～1.080 (50 ℃ of heat are surveyed), the concentrated solution spray drying gets extract 2;
With extract 1 and extract 2 mix, mixing, obtain phellinus linteus extract.
The present invention also provides a kind of pharmaceutical compositions, and this pharmaceutical compositions comprises above-mentioned phellinus linteus extract and pharmaceutically acceptable carrier.
This pharmaceutical compositions can be prepared into multiple dosage forms such as oral formulations, ejection preparation, rectal suppository, cream cream, can be oral, mode administration such as intramuscular injection, intravenous injection, subcutaneous injection, rectally.
Animal experiment proves that phellinus linteus extract of the present invention can obviously reduce the intravital blood sugar concentration of diabetic mice.Therefore, phellinus linteus extract of the present invention can be used for preparing hypoglycemic medicine, because the main foundation of treatment of diabetes is exactly blood sugar lowering, therefore, phellinus linteus extract of the present invention can be used for preparing the medicine for the treatment of diabetes.
Phellinus igniarius (L. ex Fr.) Quel. has the characteristics of Nantural non-toxic side effect as medicinal fungi, and the present invention has made full use of Phellinus igniarius (L. ex Fr.) Quel. sporophore herb by the method that first ethanol is carried, residue continuation water is carried, and the active ingredient of being rich in flavonoid and polysaccharide has the obvious functions of blood sugar effect.
Description of drawings
Fig. 1 phellinus linteus extract preparation method process route chart
Below by specific embodiment the present invention is done a detailed narration, but the present invention is not restricted to specific embodiment.Be interpreted as, all technical characterictics with the technology of the present invention feature similarity, equivalence are the scope that the present invention is contained.
The specific embodiment
The preparation of embodiment 1 phellinus linteus extract
1. the raw material decontamination cleans
The Phellinus igniarius (L. ex Fr.) Quel. sporophore is removed impurity, carry out coarse powder with the raw material Roughpulverizer and be broken into the granule shape.
2. the preparation of extract 1
Adopt under the 80% food grade ethanol room temperature and soaked 24-48 hours, solid-liquid ratio 1:15 (weight kilogram specific volume liter) filters the back and keeps supernatant, residue reuse 80% soak with ethanol 24-48 hours; Merge lixiviating solution twice, in 40 ℃ of following concentrating under reduced pressure, wait to boil off most of ethanol after; add water again and continue concentrating under reduced pressure, 50-55 ℃ of concentration tank temperature, pressure is less than 0.1MPa; be concentrated to proportion 1.050～1.080 (50 ℃ of heat are surveyed), the concentrated solution lyophilization gets phellinus linteus extract 1.
3. the preparation of extract 2
Phellinus igniarius (L. ex Fr.) Quel. sporophore residue after the soak with ethanol extraction is air-dry, fling to ethanol, add water and extracted solid-liquid ratio 1:10 (weight kilogram specific volume liter) 2 hours in 90-100 ℃; Filter and collect extracting solution, residue extracting in water more once merges extracted twice liquid, and extracting solution pumps in the concentrating under reduced pressure jar, carry out concentrating under reduced pressure, 65 ℃ of concentration tank temperature, pressure is less than 0.1MPa, be concentrated to proportion 1.050～1.080 (50 ℃ of heat are surveyed), the concentrated solution spray drying gets extract 2.
4. two extracts mix
Extract 1 and extract 2 are mixed, and mixing in mixing and blending machine obtains phellinus linteus extract, and the content of flavone is 26.7% in this extract, and the content of polysaccharide is 7.2%.
5. sterilization packaging
Phellinus linteus extract capsule automatic filling machine fill, the bottling irradiation sterilization, standby.
The hypoglycemic activity of embodiment 2 phellinus linteus extracts
1. experiment material
1.1 animal: a cleaning level male ICR mouse, 18～22g is provided by Yangzhou University comparative medicine center, the quality certification number: SCXK (Soviet Union) 2007-0001.
1.2 medicine and reagent:
Alloxan, U.S. sigma company, Lot 37 H1381; The glucose assays test kit, Shanghai Rongsheng Bioisystech Co., Ltd, lot number: 20080413; Metformin hydrochloride tablet, Kunshan Shuanghe Pharmaceutical Co., Ltd., lot number: 0604193; Phellinus linteus extract 1, phellinus linteus extract 2, phellinus linteus extract are for what prepare according to embodiment 1 method.
1.3 instrument:
The BS110S electronic balance, Beijing Sai Duolisi balance company limited; The KUBOTA-5800 centrifuge, KUBOTA Corporation; Multiskan Spectrum MSS 1500-320, ThermoElectron Corporation.
2. experimental technique
Take out 10 of mices at random as normal group; behind all the other mice fasting 14-16h; tail vein injection alloxan 60mg/kg; inject back 72 hours eye socket rear vein beards and get blood; separation of serum; prediction blood glucose; select fasting glucose〉90 of the mices of 12mmol/L; be divided into 9 groups at random; wherein 5 groups give phellinus linteus extract 1 200,400mg/kg by 10ml/kg body weight per os respectively, phellinus linteus extract 2200,400mg/kg, phellinus linteus extract 200,400mg/kg; metformin 200mg/kg, the model group per os gives isopyknic water.Once a day, successive administration is 14 days.Wherein, respectively organize fasting 4h before the 7th administration, continue fasting 2h after the administration, the mouse orbit rear vein beard is got blood then, and separation of serum is measured blood glucose.Fasting 4h before the last administration continues fasting 2h after the administration, mice is plucked eyeball and gets blood, and separation of serum is measured fasting glucose with kit method.
3. add up: experimental data is represented with X ± SD, and is represented group difference with the t inspection statistics.
4. result of the test
Compare the fasting glucose of alloxan diabetes mice extremely obviously raise (P＜0.01) with normal group.Compare with model group, after administration the 7th day, phellinus linteus extract 1 and phellinus linteus extract all can the utmost point when 200mg/kg and 400mg/kg dosage reduce the fasting glucose (P＜0.01) of diabetic mice significantly; Phellinus linteus extract 2 can reduce the fasting glucose (P＜0.05) of diabetic mice significantly at 200mg/kg, all can the utmost point when 400mg/kg dosage reduces the fasting glucose (P＜0.01) of diabetic mice significantly.After the administration 14 days, phellinus linteus extract and extract 1 and 2 all can the utmost point when 200mg/kg and 400mg/kg dosage reduce the fasting glucose (P＜0.01) of diabetic mice significantly.
Table 1 phellinus linteus extract is to the influence of alloxan diabetes mouse blood sugar
##Compare with normal group P＜0.01;
*P＜0.05,
*Compare with model group P＜0.01
Evidence, phellinus linteus extract 1, phellinus linteus extract 2 and phellinus linteus extract all have the obvious functions of blood sugar effect, and the positive control drug of metformin is a kind of medicine for the treatment of diabetes, can effectively reduce fasting blood glucose level.This result of the test shows that phellinus linteus extract and metformin all can reduce the fasting glucose of diabetic mice, statistically there was no significant difference on year-on-year basis.From the result, the blood sugar decreasing effect of metformin is more remarkable, but this is a kind of chemicals, and taking for a long time can be influential to liver, renal function, and especially the patient of liver, renal insufficiency just should not take.And phellinus linteus extract is a kind of natural product, has no side effect, and can not produce harmful effect to the patient.
Claims (6)
1. a phellinus linteus extract contains flavone and polysaccharide, and wherein weight percent hundred content of flavone are 20-30%, and weight percent hundred content of polysaccharide are 5-10%.
2. method for preparing the described extract of claim 1, this method comprises the steps:
With the soak with ethanol of Phellinus igniarius (L. ex Fr.) Quel. sporophore with 75%-90%, get supernatant, concentrate drying obtains extract 1;
After above-mentioned residual residue vapored away ethanol, the water logging bubble was got supernatant, and concentrate drying obtains extract 2;
Extract 1 and extract 2 are mixed, obtain phellinus linteus extract.
3. method as claimed in claim 2 is characterized in that this method comprises the steps:
The Phellinus igniarius (L. ex Fr.) Quel. sporophore was soaked 24-48 hours under the 75%-90% room temperature, and solid-liquid ratio 1:10-20 filters the back and keeps supernatant, residue reuse 80% soak with ethanol 24-48 hours; Merge lixiviating solution twice, in 40 ℃ of following concentrating under reduced pressure, wait to boil off most of ethanol after, add water again and continue concentrating under reduced pressure, 50-55 ℃ of concentration tank temperature, pressure is concentrated to proportion 1.050～1.080 less than 0.1MPa, the concentrated solution lyophilization gets phellinus linteus extract 1;
Phellinus igniarius (L. ex Fr.) Quel. sporophore residue after the soak with ethanol extraction is air-dry, fling to ethanol, add water and extracted solid-liquid ratio 1:5-15 at 2 o'clock in 90-100 ℃; Filter and collect extracting solution, residue extracting in water more once merges extracted twice liquid, and extracting solution pumps in the concentrating under reduced pressure jar, carry out concentrating under reduced pressure, 65 ℃ of concentration tank temperature, pressure is less than 0.1MPa, be concentrated to proportion 1.050～1.080, the concentrated solution spray drying gets extract 2;
With extract 1 and extract 2 mix, mixing, obtain phellinus linteus extract.
4. phellinus linteus extract is prepared by following method:
With the soak with ethanol of Phellinus igniarius (L. ex Fr.) Quel. sporophore with 75%-90%, get supernatant, concentrate drying obtains extract 1;
After above-mentioned residual residue vapored away ethanol, the water logging bubble was got supernatant, and concentrate drying obtains extract 2;
Extract 1 and extract 2 are mixed, obtain phellinus linteus extract.
5. a pharmaceutical compositions is characterized in that comprising claim 1 or 3 each described phellinus linteus extract and pharmaceutically acceptable carriers.
6. claim 1 or 3 each described phellinus linteus extracts are used to prepare the purposes for the treatment of diabetes medicament.
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| CNA2008102017980A CN101474211A (en) | 2008-10-27 | 2008-10-27 | Phellinus linteus extract and preparation method thereof |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102415973A (en) * | 2011-12-12 | 2012-04-18 | 李志诚 | Natural plant hair dye or dye |
| CN104163874A (en) * | 2014-07-21 | 2014-11-26 | 杭州众芝康菇生物技术有限公司 | Phellinus linteus polysaccharide ZDT and preparation method thereof |
| CN106070892A (en) * | 2016-06-30 | 2016-11-09 | 高广军 | A kind of processing method of Phellinus igniarius (L. ex Fr.) Quel. health-protecting tea extract |
| CN106491662A (en) * | 2016-11-03 | 2017-03-15 | 淳安千岛湖桑都食用菌专业合作社 | A kind of method for cultivating extraction polysaccharide and flavones simultaneously in waste material from Phellinus |
| CN106943438A (en) * | 2017-02-24 | 2017-07-14 | 浙江大学 | Phellinus active anticancer flavone compound PBF 1 and preparation method and application |
| CN107441078A (en) * | 2017-07-14 | 2017-12-08 | 中南民族大学 | A kind of pharmaceutical composition for treating diabetes and its production and use |
| CN107496466A (en) * | 2016-06-14 | 2017-12-22 | 伽蓝（集团）股份有限公司 | Phellinus active site and its preparation method and application, the skin preparations for extenal use containing it |
| CN111096983A (en) * | 2020-01-17 | 2020-05-05 | 浙江工业大学 | Phellinus igniarius phenolic extract with hypoglycemic activity and preparation and application thereof |
| CN112546077A (en) * | 2020-12-17 | 2021-03-26 | 浙江省农业科学院 | Combined extraction method and application of hypoglycemic substances in phellinus igniarius |
| CN114984066A (en) * | 2021-03-01 | 2022-09-02 | 葡萄王生技股份有限公司 | Application of phellinus igniarius in preparation of composition for improving sarcopenia |
| CN115025055A (en) * | 2022-06-14 | 2022-09-09 | 安徽利民生物科技股份有限公司 | A dispersible tablet containing Phellinus Linteus extract and its preparation method |
| US11944659B2 (en) | 2020-10-19 | 2024-04-02 | Grape King Bio Ltd | Method for improving sarcopenia by using Phellinus linteus |
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2008
- 2008-10-27 CN CNA2008102017980A patent/CN101474211A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102415973A (en) * | 2011-12-12 | 2012-04-18 | 李志诚 | Natural plant hair dye or dye |
| CN104163874A (en) * | 2014-07-21 | 2014-11-26 | 杭州众芝康菇生物技术有限公司 | Phellinus linteus polysaccharide ZDT and preparation method thereof |
| CN107496466A (en) * | 2016-06-14 | 2017-12-22 | 伽蓝（集团）股份有限公司 | Phellinus active site and its preparation method and application, the skin preparations for extenal use containing it |
| CN106070892A (en) * | 2016-06-30 | 2016-11-09 | 高广军 | A kind of processing method of Phellinus igniarius (L. ex Fr.) Quel. health-protecting tea extract |
| CN106491662A (en) * | 2016-11-03 | 2017-03-15 | 淳安千岛湖桑都食用菌专业合作社 | A kind of method for cultivating extraction polysaccharide and flavones simultaneously in waste material from Phellinus |
| CN106943438A (en) * | 2017-02-24 | 2017-07-14 | 浙江大学 | Phellinus active anticancer flavone compound PBF 1 and preparation method and application |
| CN107441078A (en) * | 2017-07-14 | 2017-12-08 | 中南民族大学 | A kind of pharmaceutical composition for treating diabetes and its production and use |
| CN107441078B (en) * | 2017-07-14 | 2018-10-26 | 中南民族大学 | A kind of pharmaceutical composition and its preparation method and application for treating diabetes |
| CN111096983A (en) * | 2020-01-17 | 2020-05-05 | 浙江工业大学 | Phellinus igniarius phenolic extract with hypoglycemic activity and preparation and application thereof |
| US11944659B2 (en) | 2020-10-19 | 2024-04-02 | Grape King Bio Ltd | Method for improving sarcopenia by using Phellinus linteus |
| CN112546077A (en) * | 2020-12-17 | 2021-03-26 | 浙江省农业科学院 | Combined extraction method and application of hypoglycemic substances in phellinus igniarius |
| CN114984066A (en) * | 2021-03-01 | 2022-09-02 | 葡萄王生技股份有限公司 | Application of phellinus igniarius in preparation of composition for improving sarcopenia |
| CN115025055A (en) * | 2022-06-14 | 2022-09-09 | 安徽利民生物科技股份有限公司 | A dispersible tablet containing Phellinus Linteus extract and its preparation method |
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Application publication date: 20090708 |