CN101464460A - Miniature biochemical detection instrument with internal calibartion function and its matched reagent - Google Patents
Miniature biochemical detection instrument with internal calibartion function and its matched reagent Download PDFInfo
- Publication number
- CN101464460A CN101464460A CNA2007101725709A CN200710172570A CN101464460A CN 101464460 A CN101464460 A CN 101464460A CN A2007101725709 A CNA2007101725709 A CN A2007101725709A CN 200710172570 A CN200710172570 A CN 200710172570A CN 101464460 A CN101464460 A CN 101464460A
- Authority
- CN
- China
- Prior art keywords
- power supply
- reagent
- analyser
- cpu processor
- operating key
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention provides a miniature biochemical detector with internal calibration, which comprises the following parts: a power supply (1), an LED (2), a CPU (3), a data printer (4), a spectrophotometer-cell (5) and an operational key (6). The detector carries out self-detection on a light source system after starting up each time, and regulates working current automatically according to a detected value. The invention further provides a matching reagent used on the miniature biochemical detector with internal calibration.
Description
Technical field:
The present invention relates to bio-detector, be specifically related to a kind of miniature biochemical detector and matched reagent thereof that has internal calibration.
Background technology:
At present, Biochemical Analyzer development both at home and abroad also reaches its maturity rapidly, as Hitachi 7600, and BECKMANLX20, BAY1650, Olympus AU1000 etc.Though these instruments all have this my measuring ability, can't realize that the oneself revises.Simultaneously, reagent producer is in process of production owing to be subjected to the influence of various factors to occur difference between batch inevitably.As the change of production environment, the change of raw material activity, the difference that operating personnel feed intake, links such as the differences between batches of raw material all can cause the difference between batch that reagent occurs.As not calibrating, the accuracy of testing result will be badly influenced for these differences.Thereby medical institutions often in use, must calibrate instrument and reagent.Medical institutions need consume a large amount of calibration object/standard items so on the one hand, quality-control product, and reagent etc. have also increased the breadboard working strength of medical institutions on the other hand, have influenced breadboard work efficiency.
Summary of the invention:
The technical problem to be solved in the present invention is to overcome above-mentioned weak point, and research and design can self-alignment bio-chemical detector.
The invention provides a kind of miniature biochemical detector that has internal calibration.
Detector of the present invention is by forming with lower member: power supply, LED luminotron, CPU, printer, colorimetric pool and operating key.
The described power supply of detector of the present invention is positioned at the left back of analyser, itself and LED luminotron, and CPU, printer, colorimetric pool, operating key links to each other.
The LED luminotron is positioned at the left front of analyser, itself and CPU, and colorimetric pool, power supply links to each other.
Printer is positioned at the right back of analyser, itself and CPU, and power supply links to each other.
CPU is positioned at the central authorities of analyser, and with power supply, LED luminotron, printer, colorimetric pool, operating key links to each other.
Operating key is positioned at the right front of analyser, and with power supply, colorimetric pool, CPU links to each other.
Colorimetric pool is positioned at the right side of analyser, and in the dead astern of operating key, it links to each other with CPU, power supply, LED luminotron, operating key.
The present invention has the using method of the miniature biochemical detector of internal calibration: detector was opened in 30 minutes, and detector will focus the detection that luminous flux is carried out in the source automatically, carried out corresponding electric current adjustment or alarm simultaneously.The operator just can detect clinical sample after according to the product operation in the product inner packaging box prompting response parameter of this project manually being revised.
Another object of the present invention has provided and has been used for the above-mentioned matched reagent with self-calibrating function that has the miniature biochemical detector of internal calibration.
Matched reagent can be alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, γ-glutamyl transferase, total bilirubin, bilirubin direct, glucose, urea nitrogen, fructose ammonia, uric acid, T-CHOL, triglyceride, creatine kinase, aPoA 1, B, lipoprotein a, lactic dehydrogenase, AMS, Phos, serum calcium, HDL-C, LDL-C, serum magnesium, AHB, albumin, total protein, creatine kinase mb type isodynamic enzyme, serum chloride or creatinine.These reagent have following feature:
One, the reaction wavelength is at 340～700nm place.
Two, the absorbance of Jian Ceing is between 0～2.5A.
Three, calibration curve is a linear model.
The use that can both match with detector of the present invention of all detectable that can satisfy these two features.The present invention has following characteristics:
1, after the each start of detector, detector will carry out the oneself to light-source system and detect, according to detected value, automatically adjust working current, adjust luminous flux F thereby play, decaying to instrument as diode (LED) luminous intensity can't adjust automatically, and instrument will be pointed out the replacing light source automatically.
2, to reagent, all test before dispatching from the factory, formulate the reaction rate factor and the reagent blank of this batch according to assay, and in the inner packaging box that the reaction rate factor of formulating and reagent blank are attached to reagent.When the client uses, only need manually to revise just passable according to the external packing prompting.
The present invention can calibrate automatically, and is easy to use, and the result is accurate.
Description of drawings:
Fig. 1 the present invention has miniature biochemical detector planar structure synoptic diagram power supply 1, LED luminotron 2, CPU processor 3, printer 4, colorimetric pool, the operating key 6 of internal calibration.
Fig. 2 example 2 testing results of the present invention, X: of the present invention batch of A azoviolet testing result, Y: of the present invention batch of B azoviolet testing result.
Embodiment:
Example 1
The present invention shown in Figure 1 has the miniature biochemical detector planar structure synoptic diagram of internal calibration.It is made of power supply 1, LED luminotron 2, CPU processor 3, printer 4, colorimetric pool 5 and operating key 6.Described power supply 1 is positioned at the left back of analyser, itself and LED luminotron 2, and CPU processor 3, printer 4, colorimetric pool 5 links to each other with operating key 6.LED luminotron 2 is positioned at the left front of analyser, itself and CPU processor 3, and colorimetric pool 5 links to each other with power supply 1.Printer 4 is positioned at the right back of analyser, and it links to each other with power supply 1 with CPU processor 3.CPU processor 3 is positioned at the central authorities of analyser, and with power supply 1, LED luminotron 2, printer 4, colorimetric pool 5 links to each other with operating key 6.Operating key is positioned at the right front of analyser, and with power supply 1, colorimetric pool 5 links to each other with CPU processor 3.Colorimetric pool 5 is positioned at the right side of analyser, and in the dead astern of operating key 6, it links to each other with CPU processor 3, power supply 1, LED luminotron 2 and operating key 6.
In use after the each start of detector, detector will carry out the oneself to light-source system and detect, according to detected value, automatically adjust working current, adjust luminous flux F thereby play, decaying to instrument as diode (LED) luminous intensity can't adjust automatically, and instrument will be pointed out the replacing light source automatically, reaches the effect of automatic calibration.
Example 2 glucose detection reagent
Reagent 1:
Contain in 1 liter of reagent
Phosphate buffer 34 grams
Peroxidase 375U/L
4-hydroxybenzoic acid, 0.6 gram
4-aminopyrine, 0.4 gram
Sodium azide 1 gram
Reagent 2:
Contain in 1 liter of reagent
Phosphate buffer 1 3.5 grams
Glucose oxidase 30KU/L
Open kit, carry out the response parameter modification, reagent is inserted carry out sample mensuration in the reagent chamber then according to the reaction rate factor K and the reagent blank of sign.Reaction wavelength 505nm, temperature of reaction 37 degree, sample and reagent 1, reagent 2 ratios are 3:200:100, the reaction time is 10 minutes.Read the absorbance changing value behind sample and the reagent reacting in 10 minutes.
Glucose detection reagent on batch reagent blank is 0.3257, and the reaction rate factor K is 1211.This batch of reagent blank is 0.3178, and the reaction rate factor K is 1268.Use batch reagent and this batch reagent and detect same sample, record absorbance and be respectively 0.6437,0. to go up batch reagent measured value be (0.6437-0.3257) * 1211, must 3851 little rubbing/liter, this batch of reagent measured value is (0.6181-0.3178) * 1268,3808 little rubbing/liter.Do not calibrate as this batch reagent, then measured value be 3541 little rubbing/liter, differ 7.01% with actual value.So, the accuracy that can effectively improve the reagent measured value by self calibration.
Example 3 bile acid detectable
Reagent 1:
Contain in 1 liter of reagent
Glycocoll 2.5 grams
Sulfo-oxidized form nicotinoyl urine purine 1.0 grams
Bovine albumin 0.1 gram
Sodium azide 1 gram
pH 4.3
Reagent 2:
Contain in 1 liter of reagent
Phosphate buffer 1 3.5 grams
Reducibility coenzyme 6 grams
The black alcohol dehydrogenase 12KU of 3 α-hydroxyl
Bovine albumin 0.1 gram
Sodium azide 1 gram
pH 9.3
The detection wavelength of TBA reagent is 405nm, and the batch reaction rate factor is 6557 on the TBA reagent, and reagent blank is 0.2987, and this batch reaction rate factor is 6513, and reagent blank is 0.2864.
Go up batch reagent and survey a sample and record the per minute absorbance and be changed to 0.0532A, then measured value is 0.0532 * 6557, promptly 34.9 little rubbing/liter
This batch of reagent is surveyed same sample and is recorded the per minute absorbance and be changed to 0.0544A, and then measured value is 0.0544 * 6513, promptly 35.4 little rubbing/liter
Two batches of reagent measured values are relatively more consistent.
Example 4 magnesium ion detectable
Reagent 1:
Contain in 1 liter of reagent
Sodium sulphite 2.0 grams
Glycocoll 0.75 gram
Ethylene glycol bis (β-amino ether)-N, N, N ', N ' tetraacethyl 0.09 gram
Sodium azide 1 gram
Reagent 2:
Contain in 1 liter of reagent
Methyl thymol blue 0.5 gram
Polyvinylpyrrolidone 0.6 gram
Sodium azide 1 gram
The detection wavelength of magnesium ion reagent is 600nm, and absorbance is between 0～1.8A.
40 serum samples are detected with azoviolet of the present invention two batches, and testing result is as follows:
Dependent equation: Y=0.9604X+0.0179
Related coefficient: R=0.998
Claims (4)
1, a kind of miniature biochemical detector that has internal calibration is characterized in that this detector is by forming with lower member: power supply (1), LED luminotron (2), CPU processor (3), printer (4), colorimetric pool (5) and operating key (6); Described power supply (1) is positioned at the left back of analyser, itself and LED luminotron (2), and CPU processor (3), printer (4), colorimetric pool (5) and operating key (6) link to each other; LED luminotron (2) is positioned at the left front of analyser, and it links to each other with CPU processor (3), colorimetric pool (5) and power supply (1); Printer (4) is positioned at the right back of analyser, and it links to each other with power supply (1) with CPU processor (3); CPU processor (3) is positioned at the central authorities of analyser, and with power supply (1), LED luminotron (2), printer (4), colorimetric pool (5) links to each other with operating key (6); Operating key (6) is positioned at the right front of analyser, links to each other with power supply (1), colorimetric pool (5) and CPU processor (3); Colorimetric pool (5) is positioned at the right side of analyser, the dead astern in operating key (6), and it links to each other with CPU processor (3), power supply (1), LED luminotron (2) and operating key (6).
2, a kind of described matched reagent that has the miniature biochemical detector of internal calibration of claim 1 that is used for is characterized in that this matched reagent by reaction substrate, toolenzyme, and protective agent, excipient, antiseptic is formed; Described reaction substrate is 4-aminopyrine, 3-hydroxyl-2,4,6-tribromo-benzene sulfonic acid, 4-hydroxybenzoic acid, reducibility coenzyme, oxidisability coenzyme, p-nitrophenol (4-NPP), 2-amino-2-methyl-1-1-propyl alcohol, glucose, methyl thymol blue, polyvinylpyrrolidone or ethylene glycol bis (β-amino ether)-N, N, N ', N ' tetraacethyl, concentration is: 0.001%～3%; Described toolenzyme is peroxidase, glutamte dehydrogenase, urease, urokinase, glucose dehydrogenase, glucose oxidase, inosine oxidase, inosine hydrolytic enzyme, cholesterol oxidase, cholesterol esterase, vitamin oxidase or the black alcohol dehydrogenase of 3 α-hydroxyl, and concentration is: 100U-50KU/L; Described protective agent is bovine serum albumin, sugarcane candy, does and reveal alcohol or half dew sugar that concentration is: 0.01%～15%; Described excipient is sugarcane candy, starch, cellulose or dried starch, and concentration is: 0.01%～20%; Described antiseptic be Sodium azide, thimerosal, general in crin, chloromycetin or erythromycin concentration be: 0.01%～2%.
3, matched reagent according to claim 2 is characterized in that this matched reagent has following feature:
(1) the reaction wavelength is at 340～700nm place;
(2) absorbance of Jian Ceing is between 0～2.5A;
(3) calibration curve is a linear model.
4, a kind of use according to claim 2 has the matched reagent of the miniature biochemical detector of internal calibration, it is characterized in that described matched reagent is an alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, γ-glutamyl transferase, total bilirubin, bilirubin direct, glucose, urea nitrogen, fructose ammonia, uric acid, T-CHOL, triglyceride, creatine kinase, aPoA 1, B, lipoprotein a, lactic dehydrogenase, AMS, Phos, serum calcium, HDL-C, LDL-C, serum magnesium, AHB, albumin, total protein, creatine kinase mb type isodynamic enzyme, serum chloride or creatinine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101725709A CN101464460A (en) | 2007-12-19 | 2007-12-19 | Miniature biochemical detection instrument with internal calibartion function and its matched reagent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101725709A CN101464460A (en) | 2007-12-19 | 2007-12-19 | Miniature biochemical detection instrument with internal calibartion function and its matched reagent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101464460A true CN101464460A (en) | 2009-06-24 |
Family
ID=40805137
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007101725709A Pending CN101464460A (en) | 2007-12-19 | 2007-12-19 | Miniature biochemical detection instrument with internal calibartion function and its matched reagent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101464460A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102918393A (en) * | 2010-06-02 | 2013-02-06 | 爱科来株式会社 | Urine analysis method, device thereof, program used in urine analysis method, and storage medium thereof |
| CN103399006A (en) * | 2013-07-29 | 2013-11-20 | 重庆医科大学 | Color RGB (red, green and blue)-component-based urine analysis device and processing method thereof |
-
2007
- 2007-12-19 CN CNA2007101725709A patent/CN101464460A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102918393A (en) * | 2010-06-02 | 2013-02-06 | 爱科来株式会社 | Urine analysis method, device thereof, program used in urine analysis method, and storage medium thereof |
| CN102918393B (en) * | 2010-06-02 | 2014-11-05 | 爱科来株式会社 | Urine analysis method, and device thereof |
| CN103399006A (en) * | 2013-07-29 | 2013-11-20 | 重庆医科大学 | Color RGB (red, green and blue)-component-based urine analysis device and processing method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Eisenthal et al. | Enzyme assays: a practical approach | |
| Zhou et al. | Raw material enzymatic activity determination: a specific case for validation and comparison of analytical methods—the example of superoxide dismutase (SOD) | |
| Tanganelli et al. | Enzymic assay of creatinine in serum and urine with creatinine iminohydrolase and glutamate dehydrogenase. | |
| Horton et al. | A substrate for deubiquitinating enzymes based on time-resolved fluorescence resonance energy transfer between terbium and yellow fluorescent protein | |
| Darrouzet-Nardi et al. | Fluorescent microplate analysis of amino acids and other primary amines in soils | |
| CN101503732B (en) | Glucose oxidase single liquid detection reagent | |
| CN109239059A (en) | A kind of glycated serum protein assay kit and its preparation method and application | |
| Calvet et al. | Rapid quantification of tryptophan and tyrosine in chemically defined cell culture media using fluorescence spectroscopy | |
| Rej et al. | Quality control in clinical chemistry: characterization of reference materials | |
| Schumann et al. | IFCC reference procedures for measurement of the catalytic concentrations of enzymes: corrigendum, notes and useful advice: International Federation of Clinical Chemistry and Laboratory Medicine (IFCC)–IFCC Scientific Division | |
| US4001089A (en) | Method for determination of triglycerides and glycerol | |
| CN101464460A (en) | Miniature biochemical detection instrument with internal calibartion function and its matched reagent | |
| Athanasiou-Malaki et al. | Kinetic-potentiometric determination of amino acids based on monitoring their reaction with dinitrofluorobenzene using a fluoride-selective electrode | |
| Fossati | Phosphate determination by enzymatic colorimetric assay | |
| Lehnus et al. | Automated procedure for kinetic measurement of total triglycerides (as glycerol) in serum with the Gilford System 3500. | |
| CN105334203A (en) | Kit for detecting enzymatic activity of biotinidase | |
| CA2361077A1 (en) | Homogeneous enzymatic assay for vitamin b6 and improvements in h2s detection | |
| CN105628636B (en) | A kind of chymotrypsin vigor testing methods | |
| Ogawa et al. | A new enzymatic method for the measurement of creatinine involving a novel ATP-dependent enzyme, N-methylhydantoin amidohydrolase | |
| US4142938A (en) | Determination of triglycerides and glycerol | |
| CN100580426C (en) | Testing apparatus and methods for quantitatively analyzing enzyme inhibitors | |
| Hollands et al. | An examination of commercial kits for the determination of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) in serum | |
| CN110484598A (en) | Application of the casein plate method in the quantitative analysis of proteinase activity | |
| Eyzaguirre | An overview on chemical modification of enzymes. The use of group-specific reagents | |
| CN110398586A (en) | Fructosamine assay kit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20090624 |