CN101457233A - 构建小球藻表达载体、转化小球藻和破壁小球藻的方法 - Google Patents
构建小球藻表达载体、转化小球藻和破壁小球藻的方法 Download PDFInfo
- Publication number
- CN101457233A CN101457233A CNA2007101793833A CN200710179383A CN101457233A CN 101457233 A CN101457233 A CN 101457233A CN A2007101793833 A CNA2007101793833 A CN A2007101793833A CN 200710179383 A CN200710179383 A CN 200710179383A CN 101457233 A CN101457233 A CN 101457233A
- Authority
- CN
- China
- Prior art keywords
- chlorella
- gene
- expression
- seq
- nitrate reductase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000195649 Chlorella <Chlorellales> Species 0.000 title claims abstract description 92
- 238000000034 method Methods 0.000 title claims abstract description 43
- 239000013604 expression vector Substances 0.000 title claims abstract description 26
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 52
- 108090000913 Nitrate Reductases Proteins 0.000 claims abstract description 40
- 230000014509 gene expression Effects 0.000 claims abstract description 36
- 241000195648 Pseudochlorella pringsheimii Species 0.000 claims abstract description 17
- 108090000848 Ubiquitin Proteins 0.000 claims abstract description 16
- 102000044159 Ubiquitin Human genes 0.000 claims abstract description 16
- 239000013598 vector Substances 0.000 claims abstract description 16
- 239000013612 plasmid Substances 0.000 claims description 45
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 32
- 101000830383 Oryctolagus cuniculus Corticostatin-3 Proteins 0.000 claims description 31
- 241000195493 Cryptophyta Species 0.000 claims description 25
- 230000009466 transformation Effects 0.000 claims description 14
- 101150099105 alien gene Proteins 0.000 claims description 11
- 238000012224 gene deletion Methods 0.000 claims description 11
- 108010059820 Polygalacturonase Proteins 0.000 claims description 10
- 239000011521 glass Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000002525 ultrasonication Methods 0.000 claims description 7
- MEIRRNXMZYDVDW-MQQKCMAXSA-N (2E,4E)-2,4-hexadien-1-ol Chemical compound C\C=C\C=C\CO MEIRRNXMZYDVDW-MQQKCMAXSA-N 0.000 claims description 5
- 108010059892 Cellulase Proteins 0.000 claims description 5
- 229940106157 cellulase Drugs 0.000 claims description 5
- 101150038264 NR gene Proteins 0.000 claims description 2
- 239000002773 nucleotide Substances 0.000 claims description 2
- 125000003729 nucleotide group Chemical group 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 abstract description 10
- 239000001963 growth medium Substances 0.000 abstract description 5
- 238000012217 deletion Methods 0.000 abstract description 4
- 230000037430 deletion Effects 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 4
- 238000010353 genetic engineering Methods 0.000 abstract description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract description 3
- 241000588724 Escherichia coli Species 0.000 abstract description 2
- 108700019146 Transgenes Proteins 0.000 abstract 1
- 239000003242 anti bacterial agent Substances 0.000 abstract 1
- 229940088710 antibiotic agent Drugs 0.000 abstract 1
- 230000004071 biological effect Effects 0.000 abstract 1
- 239000003623 enhancer Substances 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 27
- 102000004190 Enzymes Human genes 0.000 description 19
- 108090000790 Enzymes Proteins 0.000 description 19
- 229940088598 enzyme Drugs 0.000 description 19
- 241000894006 Bacteria Species 0.000 description 14
- 235000018102 proteins Nutrition 0.000 description 13
- 239000000243 solution Substances 0.000 description 13
- 239000000047 product Substances 0.000 description 12
- 239000007788 liquid Substances 0.000 description 11
- 108091008146 restriction endonucleases Proteins 0.000 description 11
- 238000012216 screening Methods 0.000 description 11
- 230000009261 transgenic effect Effects 0.000 description 11
- 241000196324 Embryophyta Species 0.000 description 10
- 229910002651 NO3 Inorganic materials 0.000 description 10
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 10
- 229910052742 iron Inorganic materials 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 10
- 238000011534 incubation Methods 0.000 description 9
- 239000002609 medium Substances 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 101001091385 Homo sapiens Kallikrein-6 Proteins 0.000 description 8
- 102100034866 Kallikrein-6 Human genes 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 8
- 238000005520 cutting process Methods 0.000 description 8
- 238000000605 extraction Methods 0.000 description 8
- 241000620209 Escherichia coli DH5[alpha] Species 0.000 description 7
- 239000012634 fragment Substances 0.000 description 7
- 238000011144 upstream manufacturing Methods 0.000 description 7
- 241000283973 Oryctolagus cuniculus Species 0.000 description 6
- 230000003385 bacteriostatic effect Effects 0.000 description 6
- ZAIPMKNFIOOWCQ-UEKVPHQBSA-N cephalexin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@@H]3N(C2=O)C(=C(CS3)C)C(O)=O)=CC=CC=C1 ZAIPMKNFIOOWCQ-UEKVPHQBSA-N 0.000 description 6
- 230000008859 change Effects 0.000 description 6
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000011156 evaluation Methods 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 235000019750 Crude protein Nutrition 0.000 description 4
- 229910021529 ammonia Inorganic materials 0.000 description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 230000000968 intestinal effect Effects 0.000 description 4
- 241000195585 Chlamydomonas Species 0.000 description 3
- 240000009108 Chlorella vulgaris Species 0.000 description 3
- 102000001398 Granzyme Human genes 0.000 description 3
- 108060005986 Granzyme Proteins 0.000 description 3
- 108010051696 Growth Hormone Proteins 0.000 description 3
- 108090000854 Oxidoreductases Proteins 0.000 description 3
- 102000004316 Oxidoreductases Human genes 0.000 description 3
- 102100038803 Somatotropin Human genes 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 238000004520 electroporation Methods 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 239000000122 growth hormone Substances 0.000 description 3
- 230000010354 integration Effects 0.000 description 3
- 210000000440 neutrophil Anatomy 0.000 description 3
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 230000001131 transforming effect Effects 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- 241000972773 Aulopiformes Species 0.000 description 2
- 241000195654 Chlorella sorokiniana Species 0.000 description 2
- 241000195628 Chlorophyta Species 0.000 description 2
- 108010002069 Defensins Proteins 0.000 description 2
- 102000000541 Defensins Human genes 0.000 description 2
- 102100031780 Endonuclease Human genes 0.000 description 2
- 108010042407 Endonucleases Proteins 0.000 description 2
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 2
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000004087 circulation Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- 235000019688 fish Nutrition 0.000 description 2
- 230000001408 fungistatic effect Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 239000012160 loading buffer Substances 0.000 description 2
- 238000011169 microbiological contamination Methods 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000013600 plasmid vector Substances 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 235000019515 salmon Nutrition 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- OBMZMSLWNNWEJA-XNCRXQDQSA-N C1=CC=2C(C[C@@H]3NC(=O)[C@@H](NC(=O)[C@H](NC(=O)N(CC#CCN(CCCC[C@H](NC(=O)[C@@H](CC4=CC=CC=C4)NC3=O)C(=O)N)CC=C)NC(=O)[C@@H](N)C)CC3=CNC4=C3C=CC=C4)C)=CNC=2C=C1 Chemical compound C1=CC=2C(C[C@@H]3NC(=O)[C@@H](NC(=O)[C@H](NC(=O)N(CC#CCN(CCCC[C@H](NC(=O)[C@@H](CC4=CC=CC=C4)NC3=O)C(=O)N)CC=C)NC(=O)[C@@H](N)C)CC3=CNC4=C3C=CC=C4)C)=CNC=2C=C1 OBMZMSLWNNWEJA-XNCRXQDQSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000195597 Chlamydomonas reinhardtii Species 0.000 description 1
- 235000007089 Chlorella vulgaris Nutrition 0.000 description 1
- 241000251730 Chondrichthyes Species 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 108010076804 DNA Restriction Enzymes Proteins 0.000 description 1
- BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 101710178122 Endoglucanase 4 Proteins 0.000 description 1
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 101710176384 Peptide 1 Proteins 0.000 description 1
- 241000269908 Platichthys flesus Species 0.000 description 1
- 241000269978 Pleuronectiformes Species 0.000 description 1
- 101710089297 Polygalacturonase 1 Proteins 0.000 description 1
- 101710089302 Polygalacturonase-2 Proteins 0.000 description 1
- 240000000037 Prosopis spicigera Species 0.000 description 1
- 235000006629 Prosopis spicigera Nutrition 0.000 description 1
- 108700005078 Synthetic Genes Proteins 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 230000010307 cell transformation Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000030279 gene silencing Effects 0.000 description 1
- 101150054900 gus gene Proteins 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 229960001438 immunostimulant agent Drugs 0.000 description 1
- 239000003022 immunostimulating agent Substances 0.000 description 1
- 230000003308 immunostimulating effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000007500 overflow downdraw method Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000003910 polypeptide antibiotic agent Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000012207 quantitative assay Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 239000012898 sample dilution Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 230000003584 silencer Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (12)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2007101793833A CN101457233B (zh) | 2007-12-12 | 2007-12-12 | 构建小球藻表达载体、转化小球藻和破壁小球藻的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2007101793833A CN101457233B (zh) | 2007-12-12 | 2007-12-12 | 构建小球藻表达载体、转化小球藻和破壁小球藻的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101457233A true CN101457233A (zh) | 2009-06-17 |
CN101457233B CN101457233B (zh) | 2011-06-15 |
Family
ID=40768353
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2007101793833A Active CN101457233B (zh) | 2007-12-12 | 2007-12-12 | 构建小球藻表达载体、转化小球藻和破壁小球藻的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN101457233B (zh) |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102219847A (zh) * | 2011-04-21 | 2011-10-19 | 中国科学院遗传与发育生物学研究所 | 防御素mNP-1及其在制备抗流感病毒药物中的应用 |
WO2011130894A1 (zh) * | 2010-04-23 | 2011-10-27 | 深圳华大基因科技有限公司 | 一种启动子sbubi1、其制备方法及用途 |
WO2011130895A1 (zh) * | 2010-04-23 | 2011-10-27 | 深圳华大基因科技有限公司 | 一种启动子siubi1、其制备方法及用途 |
WO2011130893A1 (zh) * | 2010-04-23 | 2011-10-27 | 深圳华大基因科技有限公司 | 一种启动子sbubi2、其制备方法及用途 |
CN102277379A (zh) * | 2011-08-18 | 2011-12-14 | 中国科学院遗传与发育生物学研究所 | 表达凝血因子viii的表达载体及其应用 |
CN102337217A (zh) * | 2011-09-22 | 2012-02-01 | 天津师范大学 | 小球藻生物反应器的构建方法 |
CN103397007A (zh) * | 2013-07-25 | 2013-11-20 | 中国科学院遗传与发育生物学研究所 | CeDGAT1基因及其应用 |
CN103555582A (zh) * | 2013-10-22 | 2014-02-05 | 渤海大学 | 一种用复合酶破壁小球藻的方法 |
-
2007
- 2007-12-12 CN CN2007101793833A patent/CN101457233B/zh active Active
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011130894A1 (zh) * | 2010-04-23 | 2011-10-27 | 深圳华大基因科技有限公司 | 一种启动子sbubi1、其制备方法及用途 |
WO2011130895A1 (zh) * | 2010-04-23 | 2011-10-27 | 深圳华大基因科技有限公司 | 一种启动子siubi1、其制备方法及用途 |
WO2011130893A1 (zh) * | 2010-04-23 | 2011-10-27 | 深圳华大基因科技有限公司 | 一种启动子sbubi2、其制备方法及用途 |
CN102219847A (zh) * | 2011-04-21 | 2011-10-19 | 中国科学院遗传与发育生物学研究所 | 防御素mNP-1及其在制备抗流感病毒药物中的应用 |
CN102277379A (zh) * | 2011-08-18 | 2011-12-14 | 中国科学院遗传与发育生物学研究所 | 表达凝血因子viii的表达载体及其应用 |
CN102277379B (zh) * | 2011-08-18 | 2013-07-24 | 中国科学院遗传与发育生物学研究所 | 表达凝血因子viii的表达载体及其应用 |
CN102337217A (zh) * | 2011-09-22 | 2012-02-01 | 天津师范大学 | 小球藻生物反应器的构建方法 |
CN102337217B (zh) * | 2011-09-22 | 2014-03-05 | 天津师范大学 | 小球藻生物反应器的构建方法 |
CN103397007A (zh) * | 2013-07-25 | 2013-11-20 | 中国科学院遗传与发育生物学研究所 | CeDGAT1基因及其应用 |
CN103555582A (zh) * | 2013-10-22 | 2014-02-05 | 渤海大学 | 一种用复合酶破壁小球藻的方法 |
CN103555582B (zh) * | 2013-10-22 | 2015-06-10 | 渤海大学 | 一种用复合酶破壁小球藻的方法 |
Also Published As
Publication number | Publication date |
---|---|
CN101457233B (zh) | 2011-06-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101457233B (zh) | 构建小球藻表达载体、转化小球藻和破壁小球藻的方法 | |
CN103343120B (zh) | 一种小麦基因组定点改造方法 | |
CN109694872A (zh) | 调控基因表达的方法 | |
CN108610427A (zh) | 飞蝗滞育激素基因及其在调控昆虫滞育中的应用 | |
CN103088046A (zh) | ZFNs介导的牛MSTN基因敲除和定点整合外源基因的方法 | |
CN104725496A (zh) | 一种调控植物开花的棉花WRKY转录因子GarWRKY9及应用 | |
CN109825501A (zh) | 一种来源于拟南芥的长链非编码rna t5120及其用途 | |
CN103183731B (zh) | 石斛DnMYB类转录因子、编码基因、载体、工程菌及应用 | |
CN107177603A (zh) | 烟草生长素转运蛋白NtPIN4及其应用 | |
US20210309982A1 (en) | Materials and methods for creating strains of saccharomyces cerevisiae that exhibit an increased ability to ferment oligosaccharides into ethanol | |
CN114410658B (zh) | 一种降低水稻糙米镉含量的基因OsWNK9及其编码蛋白和应用 | |
CN116284286A (zh) | 小麦条锈菌糖转运蛋白、基因及其应用 | |
CN110156883A (zh) | 烟草SLs信号转导蛋白NtDAD2及其编码基因,重组表达载体、基因编辑载体及应用 | |
CN102229940B (zh) | 一种莱茵衣藻目标基因敲除方法 | |
CN110791503B (zh) | 一种低磷诱导型启动子及其应用 | |
CN110106175B (zh) | 一种dsRNA及其在害虫防治中的应用 | |
CN102260686B (zh) | 提高小球藻中总油脂、亚油酸或油酸的含量的方法 | |
CN101955522B (zh) | 一种花生二价铁转运蛋白及其编码基因与应用 | |
CN109913480A (zh) | 一种蝗虫尿苷二磷酸葡萄糖醛酸转移酶基因及其应用 | |
CN104059938A (zh) | 桔小实蝇钠离子通道基因的rna干扰载体及其构建方法和应用 | |
CN104059931A (zh) | 桔小实蝇细胞色素p450基因的rna干扰载体及其构建方法和应用 | |
CN101280282B (zh) | 转牙鲆生长激素基因酵母及其制备方法和应用 | |
CN108148122A (zh) | 一种利用微萍作为生物反应器表达鱼类抗病原菌蛋白疫苗的方法 | |
EP2441828B1 (en) | Algal bio-flocculation by inactivation of photoreceptors | |
CN103320333B (zh) | 携带染色体分散整合木糖基因的工业酿酒酵母重组菌株 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Owner name: POLO BIOLOGY SCIENCE PARK CO., LTD. Free format text: FORMER OWNER: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCES Effective date: 20120806 |
|
C41 | Transfer of patent application or patent right or utility model | ||
COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 100101 CHAOYANG, BEIJING TO: 101113 TONGZHOU, BEIJING |
|
TR01 | Transfer of patent right |
Effective date of registration: 20120806 Address after: 101113 Beijing city Tongzhou District Zhang Wanli Si two Industrial Zone No. 1 Patentee after: Polo Biology Science Park Co., Ltd. Address before: 100101 Beijing city Chaoyang District Datun Road, building 917 Patentee before: Inst. of Genetics & Development Biology, Chinese Academy of Sciences |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20200102 Address after: 101149 No.1, Liersi village, Zhangjiawan Town, Tongzhou District, Beijing Patentee after: Beijing Sino Paul Biological Technology Co., Ltd. Address before: 101113 Beijing city Tongzhou District Zhang Wanli Si two Industrial Zone No. 1 Patentee before: Polo Biology Science Park Co., Ltd. |