CN101456773B - Edible fungus antipollution material and preparation and use method thereof - Google Patents

Edible fungus antipollution material and preparation and use method thereof Download PDF

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Publication number
CN101456773B
CN101456773B CN200810246988A CN200810246988A CN101456773B CN 101456773 B CN101456773 B CN 101456773B CN 200810246988 A CN200810246988 A CN 200810246988A CN 200810246988 A CN200810246988 A CN 200810246988A CN 101456773 B CN101456773 B CN 101456773B
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antipollution
edible fungus
materials
weight
antipollution material
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CN101456773A (en
Inventor
周建树
于虎元
鞠志珍
李莉
韩冰
赵洁
池景良
于海悦
陈超
孟庆国
庄英军
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LIAONING SCIENTIFIC ACADEMY OF MICROBIOLOGY
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LIAONING SCIENTIFIC ACADEMY OF MICROBIOLOGY
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Abstract

The invention relates to an edible fungus antipollution material and a preparation and use method thereof. The material comprises 60 to 75 weight portions of antibacterial carrier, 15 to 25 weight portions of wheat bran and 0.5 to 2 weight portions of gypsum, wherein the mixture ratio of the total weight of the three compositions to 2 to 3 percent of lime water is 1:1.2-1.3. The method comprises: stacking mixed materials, performing trepanning ventilation on stockpiles, performing stacking fermentation for 48 to 98 hours at natural temperature, and turning the piles at intervals of 20 to 24 hours during the period; laying antibacterial materials at both ends of an edible fungus cultivated material bag, and performing compression and normal-temperature or high-pressure sterilization on the antibacterial materials for standby, wherein the thickness of the antipollution materials at each end is between 5 and 10 centimeters, and the middle of the edible fungus cultivated material bag is cultivated materials; and performing open inoculation on the antipollution materials, cultivating the antipollution materials for 25 to 30 days at a temperature of between 20 and 25 DEG C, and performing sporocarp formation management after hypha penetrates through the cultivated materials. The material has antagonism on mixed bacteria; the pollution rate of the edible fungus cultivated material bag cultivated by the method is less than 1 percent and reduced by 10 percent compared with the art that no antipollution material is added; and the edible fungus antipollution material particularly has strong function of inhibiting Aspergillus niger, blue mould, Aspergillus flavus and hair mould.

Description

Edible fungus antipollution material and preparation thereof and method of use
Technical field
The present invention relates to the culture medium of edible fungus field, particularly be used for a kind of edible fungus antipollution material and the preparation and the method for use of cultivating materials.
Background technology
The edible fungus culturing mode mainly contains grog cultivation, semi-mature material cultivation, cultivation in raw material, the cultivation of fermentation material at present.
The grog cultivation: normally culturing raw material is prepared, pack then, high pressure or normal temperature sterilization, after the cooling, aseptic condition inoculation down.This method advantage is that output is high, and technology is convenient to grasp, and shortcoming is that inoculation condition is strict, if inoculation environment or culture bag sterilization are not thorough, serious contamination phenomenon can occur, causes bigger loss.Because grog cultivation output is high, so most area and mushroom farming all adopt the mode of grog cultivation to carry out edible fungus culturing.But because the grog cultivation has its fatal shortcoming, inoculation must be carried out under aseptic condition, and pollution rate is very high, has greatly limited the development of edible mushrooms, and causing to production greatly has loss.Therefore usually use some antiseptic-germicides, mould clean etc. such as derosal, gram, but therefore these antimicrobial products be not suitable for the grog cultivating method to use in case just lost anti-microbial effect through pyroprocessing,
Cultivation in raw material: this method is similar with the grog method, and different is not need high pressure or normal-pressure sterilization, and advantage is to reduce the sterilization cost; Can openly inoculate, shortcoming is that bacterial classification must be cultivated being lower than under 15 ℃ the condition, and incubation time is long; In case temperature raises; Assorted bacterium produces in a large number, causes damage, and does not generally adopt this mode to cultivate now.
The semi-mature material cultivation: cultivating materials was sterilized 2 hours under 100 ℃ of conditions, and advantage is to save the energy, and shortcoming is under coldcondition, to inoculate, otherwise is prone to pollution microbes.
The cultivation of fermentation material: with stockpile system, turning regularly utilizes biological heat energy to reach the purpose of sterilization.This method can be saved mass energy, reduces labor intensity, and can openly inoculate.Shortcoming is that fermentation technique acquires a certain degree of difficulty, and some edible fungus variety is not suitable for this method and cultivates.
Summary of the invention
The present invention be directed to the common issue with that above-mentioned cultivating method exists; Provide a kind of suitable mushroom farming operation, pollution rate low, assorted bacterium is had antagonistic action; Especially black mold, mould, flavus, Mucor there are strong restraining effect, the output height edible fungus antipollution material that cost is low again and preparation and method of use.
The edible fungus antipollution material that the present invention is prepared, the parts by weight of contained component and each component are:
Antibiotic carrier 60-75
Wheat bran 15-25
Gypsum 0.5-2
The weight proportion of above-mentioned component gross weight and 2-3% liming is 1: 1.2-1.3.
Above-mentioned antibiotic carrier comprises that wherein any one or more mixes for cotton seed hulls, wood chip, corn cob, and wheat bran can replace with Semen Maydis powder, the various rice bran that nothing is gone mouldy.
The preparation method of edible fungus antipollution material according to the invention may further comprise the steps:
1) anti-biotic material preparation: antibiotic carrier 60-75 part, wheat bran 15-25 part, gypsum 0.5-2 part;
2) liming that adds concentration expressed in percentage by weight 2-3% after the weighing is mixed thoroughly, and the weight proportion of component gross weight and liming is 1: 1.2-1.3;
3) build heap fermentation: spice is built heap, perforating ventilation on stockpile then, under natural temperature fermentation reactor system 48-96 hour, during whenever promptly process edible fungus antipollution material at a distance from turning in 20-24 hour.
The antipollution material that the present invention processes can not use if any residue at that time; Can be drying by above-mentioned anti-biotic material prescription; The antipollution material that is about to process dries, and under shady and cool drying conditions, stores, and admixes the lime boiling water of concentration expressed in percentage by weight as 2-3% when using again; The weight proportion of antipollution material and lime boiling water is 1: 1.2-1.3, and manipulate by following method.
The method of use of edible fungus antipollution material according to the invention is following:
1) this antipollution material is placed edible fungus culturing pocket two ends, the thickness of every end antipollution material is 5-10cm, and the centre is a cultivating materials, and normal temperature or autoclaving are subsequent use after the compacting;
2) adopt open inoculation edible fungus species at this antipollution material position, under 20-25 ℃ of condition, cultivated 25-30 days, after mycelia is had thorough grasp culture material, carry out management of producing mushroom.
Antipollution material of the present invention has antagonistic action to assorted bacterium; The edible mushroom cultivating bag pollution rate that uses this method to cultivate is below 1%; Especially black mold, mould, flavus, Mucor there is stronger restraining effect; In whole mycelium culture process, pollution rate reduces by 10% than the culture bag that does not add the anti-soil dyestuff; Compare easy to operate, characteristics such as pollution is little, output is high, cost is low, remarkable benefit that the present invention has with grog cultivation or cultivating edible fungus with raw material.
Embodiment
Embodiment 1: the preparation of edible fungus antipollution material
1) anti-biotic material preparation: each the component dosage by antibiotic carrier, gypsum and wheat bran compounding edible fungus antipollution material is seen table 1.
Each composition weight umber of table 1 anti-biotic material
The prescription sequence number Antibiotic carrier Gypsum Wheat bran or surrogate
1 Cotton seed hulls 60 0.8 Semen Maydis powder 20
2 Wood chip 30 corn cobs 30 1.0 Wheat bran 25
3 Cotton seed hulls 30 corn cobs 20 wood chips 20 1.5 Rice bran 20
4 Cotton seed hulls 30 corn cobs 40 1.0 Wheat bran 15
5 Corn cob 70 1.2 Wheat bran 20
2) according to the material weighing of 1-5 number any prescription in the embodiment table 1, the liming that adds weight 2-3% is mixed thoroughly, and the weight proportion of component gross weight and liming is 1: 1.2-1.3;
3) build heap fermentation: with spice build pile trapezoidal; Perforating ventilation on stockpile then, under natural temperature fermentation reactor system 48-96 hour, winter, fermentation time was 96 hours; 48-72 hour all the other seasons, during every promptly process edible fungus antipollution material at a distance from turning in 20-24 hour.
Embodiment 2: edible fungus antipollution material drying
After the edible fungus antipollution material that embodiment 1 is processed dries; Under shady and cool drying conditions, store; Admix the lime boiling water of concentration expressed in percentage by weight as 2-3% when using, the weight proportion of antipollution material and lime boiling water is 1: 1.2-1.3, and manipulate by embodiment 3 methods.
Embodiment 3: the use of edible fungus antipollution material
The method of use of edible fungus antipollution material is following:
1) this antipollution material is placed edible fungus culturing pocket two ends, the thickness of every end antipollution material is 5-10cm, and the centre is a cultivating materials, and normal temperature or autoclaving are subsequent use after the compacting;
2) bacterial classifications such as flat mushroom, Coprinus comatus, apricot spore mushroom, sliding mushroom are adopted be seeded in this anti-biotic material position under the open condition, under 20-25 ℃ of condition, cultivated 25-30 days then, after mycelia is had thorough grasp culture material, carry out management of producing mushroom.Whole mycelium culture process, pollution rate reduces by 10% than the culture bag that does not add the anti-soil dyestuff.
All kinds of edible mushroomss of table 2 are used antipollution material front and back pollution condition synopsis
Numbering Formal name used at school Chinese name Use the preceding pollution rate (%) of antipollution material Use antipollution material after stain rate (%)
CCEF89 Pleurotus ostreatus Flat mushroom 10 0.1
J002 Coprinus?comatus Coprinus comatus 10 0.3
XS-42 Pleurotus eryngii Pleurotus eryngii 15 0.5
C3-1 Pholiota?nameko Sliding mushroom 20 1.0

Claims (4)

1. edible fungus antipollution material is characterized in that the parts by weight of the contained component of this material and each component are:
Antibiotic carrier 60-75
Wheat bran 15-25
Gypsum 0.5-2
The weight proportion of above-mentioned component gross weight and 2-3% liming is 1: 1.2-1.3;
Preparing method according to this edible fungus antipollution material of above-mentioned raw materials may further comprise the steps:
1) antipollution material is prepared by weight, and the liming that adds concentration expressed in percentage by weight 2-3% after the weighing is mixed thoroughly, and the weight proportion of component gross weight and liming is 1: 1.2-1.3;
2) build heap fermentation: spice is built heap, perforating ventilation on stockpile then, under natural temperature fermentation reactor system 48-96 hour, during whenever promptly process edible fungus antipollution material at a distance from turning in 20-24 hour.
2. according to the said edible fungus antipollution material of claim 1, it is characterized in that said antibiotic carrier comprises that wherein any one or more mixes for cotton seed hulls, wood chip, corn cob, wheat bran can replace with Semen Maydis powder, the various rice bran that nothing is gone mouldy.
3. according to the preparation method of the said edible fungus antipollution material of claim 1, it is characterized in that this method may further comprise the steps:
1) antipollution material is prepared by weight: antibiotic carrier 60-75 part, wheat bran 15-25 part, gypsum 0.5-2 part;
2) liming that adds concentration expressed in percentage by weight 2-3% after the weighing is mixed thoroughly, and the weight proportion of component gross weight and liming is 1: 1.2-1.3;
3) build heap fermentation: spice is built heap, perforating ventilation on stockpile then, under natural temperature fermentation reactor system 48-96 hour, during whenever promptly process edible fungus antipollution material at a distance from turning in 20-24 hour.
4. according to the method for use of the said edible fungus antipollution material of claim 1, it is characterized in that this method of use is following:
1) this antipollution material is placed edible fungus culturing pocket two ends, the thickness of every end antipollution material is 5-10cm, and the centre is a cultivating materials, and normal temperature or autoclaving are subsequent use after the compacting;
2) adopt open inoculation edible fungus species at the antipollution material position, under 20-25 ℃ of condition, cultivated 25-30 days, after mycelia is had thorough grasp culture material, carry out management of producing mushroom.
CN200810246988A 2008-12-28 2008-12-28 Edible fungus antipollution material and preparation and use method thereof Expired - Fee Related CN101456773B (en)

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CN101456773B true CN101456773B (en) 2012-09-05

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* Cited by examiner, † Cited by third party
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CN103724081A (en) * 2013-12-08 2014-04-16 朱汉学 Oyster mushroom culture medium prepared from corn flour and wheat bran
CN111264303B (en) * 2020-04-09 2022-05-06 中华全国供销合作总社昆明食用菌研究所 Lyophyllum fumosoroseum cultivar and preparation method thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1205834A (en) * 1998-08-14 1999-01-27 潘鑫 Formulation and preparation process of champignon cultivating material

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1205834A (en) * 1998-08-14 1999-01-27 潘鑫 Formulation and preparation process of champignon cultivating material

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