CN101401958B - Chitosan-hydroxyapatite composite spheroidal particle, producing method and apparatus thereof - Google Patents
Chitosan-hydroxyapatite composite spheroidal particle, producing method and apparatus thereof Download PDFInfo
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- CN101401958B CN101401958B CN2008102350771A CN200810235077A CN101401958B CN 101401958 B CN101401958 B CN 101401958B CN 2008102350771 A CN2008102350771 A CN 2008102350771A CN 200810235077 A CN200810235077 A CN 200810235077A CN 101401958 B CN101401958 B CN 101401958B
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- spheroidal particle
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Abstract
The invention relates to a chitosan-hydroxyapatite composite spherical particle, and a method and a device for preparing the same. The method comprises the following steps: preparing a chitosan acetic acid solution at first, and then adding nanometer hydroxyapatite into the chitosan acetic acid solution to prepare a suspension liquid, wherein the mass ratio of the hydroxyapatite to the chitosan is (6-1) to (1-5). The prepared suspension liquid is added into a material storage tank from a feed opening, is forced to flow out of a liquid guiding tube evenly through controlling the pressure in the material storage tank, and is dripped into a condensate in a thermal insulation container to be condensed so as to form spherical particles. The condensed spherical particles are treated by freeze-drying at first, then are crosslinked by 2.5 percent of glutaraldehyde solution, and finally are washed by anhydrous ethanol to obtain the chitosan-hydroxyapatite composite spherical porous particle material. The spherical particle material has homogeneous particle diameter distribution, contains a large number of micropores communicated with one another therein, has higher specific surface area, and can be applied to tissue repair and medicament slow release.
Description
One, technical field
The invention belongs to a kind of technology of preparing that is applied to the spherical particle material of biomedical sector, be specifically related to a kind of chitosan-hydroxyapatite composite spheroidal particle and preparation method thereof and device.
Two, background technology
Prior art: chitin extensively is present in the cell wall of arthropodan shell such as shrimp, Eriocheir sinensis and insecticide and rudimentary plants such as bacterium, algae, is one of the abundantest biopolymer of occurring in nature, is a kind of very abundant natural regenerated resources.Chitosan be chitin take off the acetyl product, chitosan is nontoxic, good biocompatibility, biodegradable, in recent years, very noticeable in the research that can absorb aspects such as operation suture thread, hemostatic material, wound dressing material and artificial skin as natural medical polymer materials.Simultaneously, chitosan also is a kind of good hard tissue repairing material, can absorb in fracture also to be applied aspect immobilization material and the damaged packing material of bone.But because the mechanical property of chitosan is lower, its application in hard tissue repair is subjected to certain restriction.
Hydroxyapatite (Hydroxyapatite is called for short HA) is the main inorganic composition in vertebrates skeleton and the tooth, and in sclerotin, hydroxyapatite accounts for 60% greatly.The hydroxyapatite of synthetic has the inorganic constituents similar to body bone tissue, contain the necessary calcium of tissue and P elements, after implanting, under the effect of body fluid, calcium and the phosphorus material surface that can dissociate, absorbed by body tissue, and can be combined the tissue that growth makes new advances with skeleton tissue formation chemical bond.Therefore, hydroxylapatite ceramic is the excellent biological compatibility that has of generally acknowledging at present, and has bone and lure guided bone, i.e. biological activity.But the hydroxyapatite of synthetic exists with Powdered usually, because it lacks adhesive, operation is inconvenient in application, and molding is not good, and material is implanted normal generation the in back and is shifted and spills, and makes its application be subjected to certain limitation.If with chitosan and the compound preparation composite of hydroxyapatite, the good biocompatibility of existing natural macromolecular material and biodegradability have the good mechanical performance again, and the performance of material will obtain very big improvement.Chitosan-hydroxyapatite composite material also can be used for preparing the packing material of particle shape except being used for preparing the block body stephanoporate support.
Micro-sphere material has the unexistent excellent properties of a lot of irregular particles, as being difficult for causing stress concentration etc. after high flowability, high bulk density, difficult reunion, the filling.At present, reparation, the alveolar ridge of frontal resorption increase due to the filling, periodontal disease of root canal and exodontia nest, the filling of jawbone bone blister cavities, atrophic rhinitis's filling, mastoid cavity filling, shaping (as the nasus incurvus beauty treatment) reach in the damaged filling of bone with other position of skeleton, and the microsphere particle packing material is used widely.The polymer-matrix microsphere particle has multiple preparation method, and commonly used have emulsifying-chemical crosslink technique, emulsifying-solvent evaporated method and a spray drying method.The microsphere diameter distribution of using these method preparations is bigger, and packed density is big, and when being applied to tissue filling, intergranular hole is very few, is unfavorable for growing into of cambium.
Three, summary of the invention
The present invention is directed to above-mentioned technological deficiency, a kind of chitosan-hydroxyapatite composite spheroidal particle and preparation method thereof and device are provided, can prepare the chitosan-hydroxyapatite composite spheroidal particle with homogeneous particle diameter.
Technical scheme of the present invention is a kind of preparation method of chitosan-hydroxyapatite composite spheroidal particle, the particle diameter of described chitosan-hydroxyapatite composite spheroidal particle is φ 0.8～4mm, the granule body interior is that the aperture is less than the micropore of 150 μ m, intercommunication between the micropore, the porosity of chitosan-hydroxyl lime stone composite spheroidal particle is 75～92%, and the mass ratio of hydroxyapatite and chitosan is (6～1) in the granule: (1～5).
A kind of preparation method for preparing described chitosan-hydroxyapatite composite spheroidal particle, preparation process is:
The first step, taking by weighing 5～25% the chitosan that accounts for the solution gross mass, to be dissolved in mass concentration be to make chitosan-acetic acid solution in 1～5% the acetum, the mass ratio of pressing hydroxyapatite and chitosan is (6～1): (1～5) takes by weighing nanometer hydroxyapatite;
Second step added nanometer hydroxyapatite in the chitosan-acetic acid solution, fully stirred and obtained chitosan-hydroxyapatite suspension;
The 3rd step was the pipe of φ 0.5～3mm with second chitosan-hydroxyapatite suspension of preparing of step from caliber, evenly flowed out with the speed of 10～60/min, splashed into temperature for condensation in the-condensed fluid of (10～20) ℃, formed spheroidal particle; Described condensed fluid is dimethicone or vegetable oil.
The 4th step is with the condensed spherical particles separation of the 3rd step, lyophilization; It is cross-linking agent solution crosslinking Treatment 3～24h of 0.25～2.5% that spheroidal particle after the lyophilization is put into mass concentration, cleans with dehydrated alcohol then, obtains chitosan-hydroxyapatite composite spheroidal particle.Described cross-linking agent is any one in formaldehyde, glutaraldehyde, the Biformyl.
Adopt drop-condensation method of the present invention to prepare microsphere particle, the preparation facilities of chitosan-hydroxyapatite composite spheroidal particle mainly is made up of charge door, Pressure gauge, pressure-control valve, storage tank, flow control valve, catheter, condensed fluid and cool-bag.
The slurry for preparing adds storage tank from charge door, by the pressure in Pressure gauge and the pressure-control valve control storage tank, to guarantee that slurry evenly flows out from catheter.The slurry that flows out from catheter drips behind certain volume greatly mouth of pipe director, in dropping process, because that capillary effect forms is spherical, then enters the condensed fluid condensation in the cool-bag, forms spheroidal particle.According to the surface tension formula:
mg＝2πrσ
In the formula: m is drop mass; R is the extracapillary radius; σ is surface tension; G is acceleration of gravity.When density and viscosity one timing of chitosan solution, under the condition of no external interference, this method can be prepared the spheroidal particle of good sphericity, uniform particle diameter.
Beneficial effect: the present invention preparation obtain chitosan-hydroxyapatite composite spheroidal particle:
(1) contain a large amount of intercommunication micropores in the granule body interior, form the 3 D stereo network structure, this granule has higher specific surface area, is conducive to the flowing of tissue fluid of the adhesion of cell;
(2) chitosan particle has narrower particle size distribution, does not need to sieve the granular materials that processing can obtain having same particle size; The present invention be the slurry that will configure by splashing in the freezing in advance liquid, the surface tension of using liquid is condensed into a kind of process of spheroidal particle.According to the surface tension formula:
mg＝2πrσ
In the formula: m is drop mass; R is the extracapillary radius; σ is surface tension; G is acceleration of gravity.When density and viscosity one timing of slurry, under the condition of no external interference, this method can be prepared the microsphere particle of good sphericity, uniform particle diameter.
(3) because the narrow diameter distribution of the spheroidal particle of the present invention preparation, and the spherical granular material of same particle size can obtain largest inter-particle porosity after the filling, so when being applied to tissue filling, be conducive to growing into of cambium.
(4) granular materials is made up of chitosan and nanometer hydroxyapatite, has both possessed good degradation capability, has possessed biological activity again.
Four, description of drawings:
Fig. 1 spheroidal particle forming device sketch map.
Device mainly is made up of charge door 1, Pressure gauge 2, pressure-control valve 3, storage tank 4, flow control valve 5, catheter 6, condensed fluid 7 and cool-bag 8.
Fig. 2 is the photo of chitosan-hydroxyapatite composite spherical honeycombed grain.
Fig. 3 is the internal void photo of chitosan-hydroxyapatite composite spherical honeycombed grain.
Five, the specific embodiment:
Embodiment 1:
(1) taking by weighing the chitosan that accounts for solution gross mass 5%, to be dissolved in mass concentration be in 1% the acetum, to be mixed with chitosan-acetic acid solution, and the mass ratio by hydroxyapatite and chitosan is that 2:1 takes by weighing nanometer hydroxyapatite then;
(2) nanometer hydroxyapatite is added in the chitosan-acetic acid solution, fully stir and obtain chitosan-hydroxyapatite suspension;
(3) shown in Figure 1, chitosan-hydroxyapatite the suspension for preparing is added storage tank 4 from charge door 1, the pressure of controlling in the storage tanks 4 by Pressure gauge 2 and pressure-control valve 3 is 0.08MPa, to guarantee that chitosan-hydroxyapatite suspension is that the speed with 10/min evenly flows out the catheter 6 of φ 0.5mm from caliber, flow velocity can be controlled by flow control valve 5, solution splashes into condensation in the condensed fluid 7 in the cool-bag 8, the temperature of condensed fluid is-10 ℃, condensed fluid is vegetable oil, forms spheroidal particle;
(4) condensed microsphere separates, and handle 8h-5 ℃ temperature lyophilization, it is 0.25% glutaraldehyde solution crosslinking Treatment 3h that spheroidal particle after the lyophilization is put into mass concentration earlier, the reuse dehydrated alcohol cleans, obtain chitosan-hydroxyapatite composite spheroidal particle, as shown in Figure 2, the good sphericity of granule, uniform particle diameter, particle diameter are φ 0.8mm, as shown in Figure 3, the granule body interior is that the aperture is less than the micropore of 150 μ m, intercommunication between the micropore forms the 3 D stereo network structure, and porosity is 75%.
Embodiment 2:
(1) taking by weighing the chitosan that accounts for solution gross mass 15%, to be dissolved in mass concentration be to be mixed with chitosan-acetic acid solution in 3% the acetum, and the mass ratio by hydroxyapatite and chitosan is that 6:1 takes by weighing nanometer hydroxyapatite then;
(2) nanometer hydroxyapatite is added chitosan-acetic acid solution, fully stir and obtain chitosan-hydroxyapatite suspension;
(3) prepared suspension liquid adds storage tank 4 from charge door 1, the pressure of controlling in the storage tanks 4 by Pressure gauge 2 and pressure-control valve 3 is 0.5MPa, to guarantee that chitosan-hydroxyapatite suspension is that the speed with 60/min evenly flows out the catheter 6 of φ 3mm from caliber, flow velocity can be controlled by flow control valve 5, solution splashes into condensation in the condensed fluid 7 in the cool-bag 8, the temperature of condensed fluid is-20 ℃, and condensed fluid is dimethicone, forms spheroidal particle;
(4) condensed microsphere separates, and handle 14h-7 ℃ temperature lyophilization, it is 1.5% glyoxal solution crosslinking Treatment 14h that spheroidal particle after the lyophilization is put into mass concentration earlier, the reuse dehydrated alcohol cleans, obtain chitosan-hydroxyapatite composite spheroidal particle, particle diameter is φ 4mm, and the granule body interior is that the aperture is less than the micropore of 150 μ m, intercommunication between the micropore, porosity are 92%.
Embodiment 3:
(1) taking by weighing the chitosan that accounts for solution gross mass 25%, to be dissolved in mass concentration be that 5% acetum is made chitosan-acetic acid solution, is that 2:5 takes by weighing nanometer hydroxyapatite by the mass ratio of hydroxyapatite and chitosan;
(2) nanometer hydroxyapatite is added chitosan-acetic acid solution, fully stir and obtain suspension;
(3) prepared suspension liquid adds storage tank 4 from charge door 1, the pressure of controlling in the storage tanks 4 by Pressure gauge 2 and pressure-control valve 3 is 1.08MPa, to guarantee that chitosan-hydroxyapatite suspension is that the speed with 40/min evenly flows out the catheter 6 of φ 1.5mm from caliber, flow velocity can be controlled by flow control valve 5, solution splashes into condensation in the condensed fluid 7 in the cool-bag 8, the temperature of condensed fluid is-15 ℃, and condensed fluid is vegetable oil, forms spheroidal particle;
(4) condensed microsphere separates, and handle 24h-10 ℃ temperature lyophilization, it is 2.5% formalin crosslinking Treatment 24h that spheroidal particle after the lyophilization is put into mass concentration earlier, the reuse dehydrated alcohol cleans, obtain chitosan-hydroxyapatite composite spheroidal particle, particle diameter is φ 2mm, and the granule body interior is that the aperture is less than the micropore of 150 μ m, intercommunication between the micropore, porosity are 82%.
Claims (4)
1. a chitosan-hydroxyapatite composite spheroidal particle is characterized in that the particle diameter of described chitosan-hydroxyapatite composite spheroidal particle is
0.8～4mm, the granule body interior be the aperture less than the micropore of 150 μ m, intercommunication between the micropore, the porosity of chitosan-hydroxyapatite composite spheroidal particle is 75～92%, the mass ratio of hydroxyapatite and chitosan is (6～1) in the granule: (1～5).
2. preparation method for preparing the described chitosan-hydroxyapatite composite spheroidal particle of claim 1 is characterized in that preparation process is:
The first step, taking by weighing 5～25% the chitosan that accounts for the solution gross mass, to be dissolved in mass concentration be to make chitosan-acetic acid solution in 1～5% the acetum, be (6～1) by the mass ratio of hydroxyapatite and chitosan: (1～5) takes by weighing nanometer hydroxyapatite;
Second step added nanometer hydroxyapatite in the chitosan-acetic acid solution, fully stirred and obtained chitosan-hydroxyapatite suspension;
In the 3rd step, chitosan-hydroxyapatite suspension that second step was prepared from caliber is
0.5 the pipe of～3mm evenly flows out with the speed of 10～60/min, splashes into temperature for condensation in the-condensed fluid of (10～20) ℃, forms spheroidal particle;
The 4th step is with the condensed spherical particles separation of the 3rd step, lyophilization; It is cross-linking agent solution crosslinking Treatment 3～24h of 0.25%～2.5% that spheroidal particle after the lyophilization is put into mass concentration, cleans with dehydrated alcohol then, obtains chitosan-hydroxyapatite composite spheroidal particle.
3. the preparation method of chitosan-hydroxyapatite composite spheroidal particle as claimed in claim 2 is characterized in that, described condensed fluid is dimethicone or vegetable oil.
4. the preparation method of chitosan-hydroxyapatite composite spheroidal particle as claimed in claim 2 is characterized in that, described cross-linking agent is any one in formaldehyde, glutaraldehyde, the Biformyl.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2018119320A1 (en) * | 2016-12-21 | 2018-06-28 | Cresilon, Inc. | Hemostatic compositions with antifibrinolytic agents |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112012000472A2 (en) * | 2009-07-09 | 2016-02-23 | Polymers Crc Ltd | hybrid biopolymer gel-deposit release system |
| ITVR20110069A1 (en) | 2011-04-06 | 2012-10-07 | Eurocoating S P A | METHOD FOR THE REALIZATION OF A BIOMATERIAL BASED ON CALCIUM PHOSPHATE UNDER THE FORM OF GRANULES AND / OR THEIR AGGREGATES AND BIOMATERIAL OBTAINED BY THE SAME |
| GB201417020D0 (en) * | 2014-09-26 | 2014-11-12 | Univ Leeds And Univ St Andrews The | Formulation |
| CN105148320A (en) * | 2015-09-28 | 2015-12-16 | 河南工程学院 | Preparing method and application of medicine carrying hydroxyapatite porous microspheres |
| CN110564040A (en) * | 2019-09-23 | 2019-12-13 | 泉州宁遇新材料有限公司 | Natural anti-corrosion and anti-oxidation HDPE plastic and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5810988A (en) * | 1994-09-19 | 1998-09-22 | Board Of Regents, University Of Texas System | Apparatus and method for generation of microspheres of metals and other materials |
| CN1272383A (en) * | 2000-05-19 | 2000-11-08 | 清华大学 | Preparation method of nanometer phase calcium-phosphorus salt/collagen/polylactic acid bone composite material |
| CN1546181A (en) * | 2003-12-12 | 2004-11-17 | 清华大学 | Degradable material capable of guiding the regeneration and renovation process of hard tissue and its preparation |
| CN1607033A (en) * | 2003-10-15 | 2005-04-20 | 中国科学院过程工程研究所 | Chitose microsphere and microcapsule with uniform size and its preparation method |
| CN1931378A (en) * | 2006-09-29 | 2007-03-21 | 清华大学 | Micro rack for bone tissue engineering and its prepn process and application |
-
2008
- 2008-11-07 CN CN2008102350771A patent/CN101401958B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5810988A (en) * | 1994-09-19 | 1998-09-22 | Board Of Regents, University Of Texas System | Apparatus and method for generation of microspheres of metals and other materials |
| CN1272383A (en) * | 2000-05-19 | 2000-11-08 | 清华大学 | Preparation method of nanometer phase calcium-phosphorus salt/collagen/polylactic acid bone composite material |
| CN1607033A (en) * | 2003-10-15 | 2005-04-20 | 中国科学院过程工程研究所 | Chitose microsphere and microcapsule with uniform size and its preparation method |
| CN1546181A (en) * | 2003-12-12 | 2004-11-17 | 清华大学 | Degradable material capable of guiding the regeneration and renovation process of hard tissue and its preparation |
| CN1931378A (en) * | 2006-09-29 | 2007-03-21 | 清华大学 | Micro rack for bone tissue engineering and its prepn process and application |
Non-Patent Citations (1)
| Title |
|---|
| 卢勰炜等.新型壳聚糖-海藻酸钠胃漂浮小丸的制备.《中国现代应用医学杂志》.2001,第21卷(第6期), * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018119320A1 (en) * | 2016-12-21 | 2018-06-28 | Cresilon, Inc. | Hemostatic compositions with antifibrinolytic agents |
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