CN101381703A - Method and equipment for improving dissolution of cell contents - Google Patents
Method and equipment for improving dissolution of cell contents Download PDFInfo
- Publication number
- CN101381703A CN101381703A CNA2008101371658A CN200810137165A CN101381703A CN 101381703 A CN101381703 A CN 101381703A CN A2008101371658 A CNA2008101371658 A CN A2008101371658A CN 200810137165 A CN200810137165 A CN 200810137165A CN 101381703 A CN101381703 A CN 101381703A
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- cavitation
- solvent
- mixture
- rotor
- entocyte
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- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000004090 dissolution Methods 0.000 title abstract 5
- 238000002156 mixing Methods 0.000 claims abstract description 7
- 239000000725 suspension Substances 0.000 claims abstract description 6
- 241001465754 Metazoa Species 0.000 claims abstract description 5
- 244000005700 microbiome Species 0.000 claims abstract description 5
- 238000010992 reflux Methods 0.000 claims abstract description 4
- 239000000203 mixture Substances 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 13
- 239000002904 solvent Substances 0.000 claims description 12
- 238000000605 extraction Methods 0.000 claims description 10
- 230000000694 effects Effects 0.000 claims description 7
- 239000003960 organic solvent Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 2
- 230000003647 oxidation Effects 0.000 claims description 2
- 238000007254 oxidation reaction Methods 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 230000000977 initiatory effect Effects 0.000 claims 1
- 230000000813 microbial effect Effects 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- 210000004027 cell Anatomy 0.000 abstract description 7
- 210000002421 cell wall Anatomy 0.000 abstract description 7
- 239000002245 particle Substances 0.000 abstract description 7
- 241000196324 Embryophyta Species 0.000 abstract description 3
- 210000000170 cell membrane Anatomy 0.000 abstract description 2
- 230000001939 inductive effect Effects 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 235000018927 edible plant Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 229930003944 flavone Natural products 0.000 description 2
- 150000002213 flavones Chemical class 0.000 description 2
- 235000011949 flavones Nutrition 0.000 description 2
- 150000008442 polyphenolic compounds Chemical class 0.000 description 2
- 235000013824 polyphenols Nutrition 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 235000011201 Ginkgo Nutrition 0.000 description 1
- 235000008100 Ginkgo biloba Nutrition 0.000 description 1
- 244000194101 Ginkgo biloba Species 0.000 description 1
- CWHJIJJSDGEHNS-MYLFLSLOSA-N Senegenin Chemical compound C1[C@H](O)[C@H](O)[C@@](C)(C(O)=O)[C@@H]2CC[C@@]3(C)C(CC[C@]4(CCC(C[C@H]44)(C)C)C(O)=O)=C4[C@@H](CCl)C[C@@H]3[C@]21C CWHJIJJSDGEHNS-MYLFLSLOSA-N 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 235000019994 cava Nutrition 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000010304 firing Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000012633 leachable Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000000633 nuclear envelope Anatomy 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 238000005381 potential energy Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000009871 tenuigenin Substances 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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- Extraction Or Liquid Replacement (AREA)
Abstract
The invention discloses a method for greatly increasing the dissolution of cell contents in animals, plants and microorganisms, which comprises the following steps: pre-crushing, soaking and mixing, cavitation crushing and reflux circulation. The cavitation crushing is to use a specially designed machine to carry out cavitation treatment on the suspension, and the special design generates a large amount of bubble clusters in the passing suspension in a mechanical rotation mode to form strong cavitation impact force, wherein the impact force can crush particles in the suspension, accelerate dissolution, be more homogeneous, reduce the viscosity of a system, and break cell walls or cell membranes so as to promote cell contents to flow out more easily, so that the dissolution amount and the dissolution efficiency are greatly improved. The invention also discloses equipment for inducing cavitation.
Description
Technical field
The present invention relates to biological substance and extract the field, is to produce cavitation with mechanical means, thereby and with this impel cell wall breaking, breaking makes effusive method of content and technology in the cell.
Background technology
Biological cell is made up of cytolemma, tenuigenin, nucleus by cellularity, and vegetable cell also has hard cell walls.At present, the mankind mainly are that material is applied in the cell membrane, and sending out alcohol etc. as the extract in Chinese medicinal materials effective ingredient, the natural goods, microorganism all is to utilize entocyte, but the complete structure of cell but produces greatly this and hinders.Often use the method for extracting entocyte that decocting method, heat reflow method, ultrasonic extraction, supercritical CO are arranged now
2, method such as Mierocrystalline cellulose enzyme process, mechanical crushing method, solvent stripping, these all are based on the destruction cell walls, make the effusive mode of entocyte, have all that extraction yield is low, extraction time is long, energy consumption is serious, environmental pollution is big, extraction efficiency is low, can't realize the shortcoming extracted continuously.For raw material, pulverize tinyly more, more homogeneous then contacts more abundant with solvent in solvent; The raw cell cell walls destroys the just many more of big more then entocyte stripping, and extraction yield and extraction efficiency are just high more.
When blade rotates in water, can produce cavitation phenomenons, this is because pressure change causes producing bubble and instant failure closure in the water, produces surging force thus blade is damaged, and this phenomenon is called " cavitation effect ".A series of dynamic processes such as micro-bubble when cavitation takes place in liquid nuclear vibrates under pressure, growth, contraction and collapse, in short period of time, around cavitation bubble, produce high temperature, high pressure, intense impact ripple and the microjet more than the speed per hour 400km/h at the utmost point that collapses simultaneously.The peeling off of solid surface, etchback and porphyrization have been created new active surface, and this interfacial effect makes that mass transfer surfaces is long-pending to be increased.The turbulence effect that produces during cavitation makes mass transfer frictional belt attenuation in the solid-liquid interface, causes the concentration gradient minimizing speed of solute in the interfacial layer to be much higher than additive method.The perturbation effect that cavitation produces is strengthened the micropore diffusion of solid-liquid mass transfer process, makes the eddy current diffusion strengthen, and accelerates sepn process.Produce cavitation effect in being mixed with the liquid of raw material, the moment of breaking of the feasible thing cell that is broken finishes, and this has greatly shortened the broken time, and produced simultaneously oscillating action has been strengthened release, diffusion and the dissolving of intracellular matter, can significantly improve extraction efficiency.The clarifixator that often uses in the production also can produce cavitation phenomenon when working, but quantity and intensity that its produces are all very little, and clarifixator mainly still utilizes the principle of mechanical shear stress to carry out work.
Summary of the invention
The object of the present invention is to provide a kind of method and apparatus that improves entocyte stripping in animal, plant, the microorganism,
Above-mentioned purpose of the present invention realizes by following technical solution, comprises the steps:
1, will be carried thing earlier such as animal and plant tentatively is crushed to 0.1mm~5mm,, directly be entered for second step if microorganism then needn't this step.
2, the material after will pulverizing enters in the blend tank, adds solvent in simultaneously jar, and solvent is water or organic solvent or its mixture.Material and solvent ratio are 1:0.5~1:10000.
3, the mixture of material and solvent is delivered in the cavitation producer, material is pulverized by the surging force of " cavitation effect " in the cavitation producer, make it particle thinner, more even, reduce system viscosity, smash cell walls simultaneously and impel the easier stripping of entocyte simultaneously, thereby increase substantially the discharge of entocyte and flow out efficient and improve the dissolving of entocyte in solvent.
4, the mixture that comes out from the cavitation producer has two whereabouts, one is back in the mixing tank again as circulation, the backflow ratio can be 0~100 adjusting, even all reflux, carry out periodical operation, so promptly guarantee the even discharging of leachable, realized switching between different varieties the handiness of producing again; Another whereabouts of mixture is to enter next step abstraction process.
The service temperature that the above-mentioned step gathers for organic solvent, in order to prevent some entocyte oxidation, can operated below 0 ℃ between-30 ℃~100 ℃.The general operation temperature is 15 ℃~33 ℃.
Another object of the present invention provides the equipment of realizing above-mentioned steps 3
This purpose of the present invention is achieved by the following technical solution:
The cavitation producer utilizes custom-designed machine to produce the cavitation bubble cluster and realizes.It is formed by having specially designed stator and rotor and cavity, and rotor is by power wheel drive, rotor have toothed structure and and stator between the space very little.The suspension that contains material axially enters machine and flows through and radially flow out machine behind the space between rotor and stator.That part of fluid internal pressure in this process during the rotor of suspension contact high speed rotating can rapid drawdown, the gas that causes liquid to reach boiling point and dissolve wherein can produce a large amount of micro-bubble clusters because of pressure reduces, when liquid flow was crossed rotor, pressure recovery was normal, and bubble can collapse closure.The cavity quantity that produces in the liquid is many, having millions of cavity in the cavity group in p.s. produces and caves in, so the surging force that the cavity group is caused is huge, in the solid particulate boundary collapse of bubbles near bubble is heterogeneous, this causes producing the jet that penetrates bubble, it is converted into bubble potential energy the kinetic energy of spouting of liquid, firing rate reaches 400km/h, it is very big to the destruction on material particles surface that this has very high-octane surging force, it can will be crushed to below 5 microns, and be evenly distributed in very much in the liquid, the viscosity of mixture system declines to a great extent and makes cell walls in the raw material simultaneously, cytolemma, nuclear membrane breaks in a large number, and makes entocyte matter be easier to stripping.
Description of drawings
Fig. 1 is cavitation generator architecture figure
Fig. 2 is entocyte dissolving-out process figure
Embodiment
Example 1: earlier ginkgo leaf powder is broken to the particle below 5 millimeters, mixing its part by weight with 50% ethanol is 1:20, get 500 kilograms of this mixtures, put into mixing tank, service temperature is 26 ℃, per hour delivers to the treatment solution scale of construction and is in 10 cubic metres the cavitation producer, and the backflow ratio is 50%, from the effluent sampling analysis, its total flavones yield is 10.39%.Similarity condition common process reflux extraction secondary, each 3 hours, its total flavones yield only was 5.8%, the viscosity ratio common process of mixture system descends 16% simultaneously, is 3~5 microns with microscopic examination particle median size, and is evenly distributed.
Example 2: earlier fresh tea leaf in its is crushed to the particle below 5 millimeters, mixing its part by weight with water is 1:10, get 500 kilograms of this mixtures, put into mixing tank, service temperature is 25 ℃, per hour delivers to the treatment solution scale of construction and is in 10 cubic metres the cavitation producer, and the backflow ratio is 10%, from the effluent sampling analysis, its bitter edible plant polyphenol extraction yield is 28.5%.Similarity condition common process poach 30 minutes, its bitter edible plant polyphenol extraction yield is 15.8%, is 3~5 microns with microscopic examination particle median size, and is evenly distributed.
Claims (5)
1, a kind of method that improves the entocyte stripping comprises the steps:
(1) will be carried thing earlier such as animal and plant tentatively is crushed to 0.1mm~5mm,, directly be entered for second step if microorganism then can this step.
(2) material after will pulverizing enters in the blend tank, adds solvent in simultaneously jar, and material and solvent ratio are 1:0.5~1:10000.
(3) mixture of material and solvent is delivered in the cavitation producer, material is pulverized by the surging force of " cavitation effect " in the cavitation producer.
(4) mixture that comes out from the cavitation producer has two whereabouts, gets back to again in the mixing tank as refluxing for one, and the backflow ratio is 0~100 adjusting; Another whereabouts of mixture is to enter next step separation and Extraction operation.
The service temperature that the above-mentioned step gathers for organic solvent, in order to prevent some entocyte oxidation, can operated below 0 ℃ between-30 ℃~100 ℃.The general operation temperature is 15 ℃~33 ℃.
2, a kind of method that improves the entocyte stripping according to claim 1 is characterized in that: the described cavitation producer of step (3) can be a large amount of micro-bubble and the hole groups of flowing through wherein of the inner initiation of fluid.
3, cell according to claim 1 is for constituting animal, plant, microbial cell.
4, solvent according to claim 1 is the mixture of water, organic solvent, water and organic solvent or the mixture of different organic solvents.
5, equipment according to claim 2, it is characterized in that: the cavitation producer is formed by having specially designed stator and rotor and cavity, rotor have toothed structure and and stator between the space very little, rotor is by power wheel drive, and the suspension that contains material axially enters machine and flows through and radially flow out machine behind the space between rotor and stator.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CNA2008101371658A CN101381703A (en) | 2008-09-23 | 2008-09-23 | Method and equipment for improving dissolution of cell contents |
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CNA2008101371658A CN101381703A (en) | 2008-09-23 | 2008-09-23 | Method and equipment for improving dissolution of cell contents |
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CNA2008101371658A Pending CN101381703A (en) | 2008-09-23 | 2008-09-23 | Method and equipment for improving dissolution of cell contents |
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Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102350267A (en) * | 2011-08-31 | 2012-02-15 | 浙江工业大学 | Trailing-vortex cavitation rotating generator |
CN103045467A (en) * | 2011-10-13 | 2013-04-17 | 徐仰德 | Biomedicine homogenizing apparatus |
CN103058979A (en) * | 2013-01-17 | 2013-04-24 | 南京林业大学 | Method for extracting procyanidine in ginkgo leaves |
CN105420092A (en) * | 2015-12-14 | 2016-03-23 | 江苏大学 | Cavitation impinging stream microalga cell wall-breaking device |
CN105481053A (en) * | 2015-12-04 | 2016-04-13 | 哈尔滨工程大学 | Screwed open hole type cavitator |
CN106902930A (en) * | 2017-02-16 | 2017-06-30 | 中南大学 | A kind of sludge organism body cell means for breaking walls and its method for sludge organism body cell broken wall |
CN110769688A (en) * | 2017-04-07 | 2020-02-07 | 阿西姆普托特有限公司 | Cryopreservation apparatus and method |
CN111821718A (en) * | 2020-04-29 | 2020-10-27 | 漳州职业技术学院 | Magnolia liliiflora polysaccharide strontium antiallergic agent production line and preparation method thereof |
CN116042302A (en) * | 2022-05-04 | 2023-05-02 | 新纪元食品科技(佛山)有限公司 | Cavitation treatment process for complete material |
-
2008
- 2008-09-23 CN CNA2008101371658A patent/CN101381703A/en active Pending
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102350267A (en) * | 2011-08-31 | 2012-02-15 | 浙江工业大学 | Trailing-vortex cavitation rotating generator |
CN103045467A (en) * | 2011-10-13 | 2013-04-17 | 徐仰德 | Biomedicine homogenizing apparatus |
CN103045467B (en) * | 2011-10-13 | 2014-05-14 | 徐仰德 | Biomedicine homogenizing apparatus |
CN103058979A (en) * | 2013-01-17 | 2013-04-24 | 南京林业大学 | Method for extracting procyanidine in ginkgo leaves |
CN103058979B (en) * | 2013-01-17 | 2014-09-24 | 南京林业大学 | Method for extracting procyanidine in ginkgo leaves |
CN105481053A (en) * | 2015-12-04 | 2016-04-13 | 哈尔滨工程大学 | Screwed open hole type cavitator |
CN105420092A (en) * | 2015-12-14 | 2016-03-23 | 江苏大学 | Cavitation impinging stream microalga cell wall-breaking device |
CN106902930A (en) * | 2017-02-16 | 2017-06-30 | 中南大学 | A kind of sludge organism body cell means for breaking walls and its method for sludge organism body cell broken wall |
CN110769688A (en) * | 2017-04-07 | 2020-02-07 | 阿西姆普托特有限公司 | Cryopreservation apparatus and method |
US11576373B2 (en) | 2017-04-07 | 2023-02-14 | Asymptote Ltd. | Cryopreservation apparatus and methods |
CN111821718A (en) * | 2020-04-29 | 2020-10-27 | 漳州职业技术学院 | Magnolia liliiflora polysaccharide strontium antiallergic agent production line and preparation method thereof |
CN116042302A (en) * | 2022-05-04 | 2023-05-02 | 新纪元食品科技(佛山)有限公司 | Cavitation treatment process for complete material |
CN116042302B (en) * | 2022-05-04 | 2024-03-26 | 新纪元食品科技(佛山)有限公司 | Cavitation treatment process for complete material |
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Application publication date: 20090311 |