CN101380103A - Dendrobium officinale extracturm and preparation method and use thereof - Google Patents
Dendrobium officinale extracturm and preparation method and use thereof Download PDFInfo
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- CN101380103A CN101380103A CNA200810121452XA CN200810121452A CN101380103A CN 101380103 A CN101380103 A CN 101380103A CN A200810121452X A CNA200810121452X A CN A200810121452XA CN 200810121452 A CN200810121452 A CN 200810121452A CN 101380103 A CN101380103 A CN 101380103A
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Abstract
The invention provides a dendrobium candidum extract, comprising the materials with the weight percentage: 10-70 % of dendrobium candidum, 10-60% of reishi shell-broken spore powder and 10-60 % of American ginseng; reishi shell-broken spore powder is prepared by breaking the cell walls of reishi spores. The beneficial effect of the invention lies in that the dendrobium candidum extract has high content of active ingredients as well as durable, stable and mild function, can improve the immunity of the human body and is convenient to carry and eat.
Description
(1) technical field
The present invention relates to a kind of dendrobium officinale extracturm, particularly is dendrobium officinale extracturm of primary raw material and its production and application with Tiepi Fengdou, glossy ganoderma spore powder with crushed sporoderm and American Ginseng.
(2) background technology
Along with the progress and development of society, increasing people begins to pursue nature, recovers one's original simplicity.But fast pace, high efficiency modern society, usually make people be in dog-eat-dog for a long time, at heart in the tense situation of big, the overload operation of pressure, this state is easy to produce the disease of physiology and psychology as can not in time dissolving.Many people that are in sub-health state begin to pay attention to self health care.The dendrobium candidum that is described as " first of the nine immortal grass " has the effect that strengthens immunity, alleviating physical fatigue, but because the restriction of formulation and production technology, in the market dendrobium candidum health products prescription and technology are reasonable inadequately, cause the Tiepi Fengdou Related product to add too much auxiliary material, active constituent content is few, can not satisfy the demand that current consumer's light, nourishing and build are given birth to.
(3) summary of the invention
The object of the present invention is to provide a kind of active constituent content height, effect stable lasting, be easy to carry, dendrobium officinale extracturm (clear cream) that taking convenience, effect relax, can strengthen immunity of organisms.
The technical solution used in the present invention is:
A kind of dendrobium officinale extracturm, the raw material quality for preparing described dendrobium officinale extracturm is composed as follows:
Tiepi Fengdou 10～70%
Glossy ganoderma spore powder with crushed sporoderm 10～60%
American Ginseng 10～60%;
Described glossy ganoderma spore powder with crushed sporoderm is that Reishi sporule obtains through breaking-wall cell.
Tiepi Fengdou: for after the stem of orchid dendrobium candidum Dendrobium candidum Xall.ex Lindl. cuts off the part fibrous root, be twisted into spirality or spring-like while frying, oven dry is practised and is claimed " Tiepi Fengdou ".Nature and flavor: sweet, be slightly cold; Function: should stomach promote the production of body fluid, nourishing Yin and clearing heat.Experimental study proof dendrobium candidum contains abundant water-soluble polysaccharide.This polysaccharide has the effect that promotes immune system lymphocyte generation migration inhibition factor, promotes SPL increment transformation.Dendrobium officinale polysaccharide not only can improve the immunocompetence of body, but also has anti-tumor bioactivity.Its anti-swollen direct killing effect that mechanism is different from common cell class medicine of heating up in a steamer, but, reach the purpose that suppresses tumor growth by improving body's immunological function.
Glossy ganoderma spore powder with crushed sporoderm: for the sporinite of the red sesame Ganoderma of Polyporaceae fungi Lucidum (Leyss.exFr) Karst processes through broken wall.Lucidum spore powder is after ganoderma lucidum fruitbody is reached maturity, and collects by the tube spore that penetrates of being hit by a bullet to form.Contain several amino acids, inorganic nutritive element, triterpene compound, alkaloid, phytosterin compound, macromolecule polysaccharide and other many physiological activators in the lucidum spore powder.Lucidum spore powder has the function of the immunity regulated, and the phagocytic function of energy activating macrophage has antagonism to glucocorticoid.Because Reishi sporule has double-deck tough and tensile outer wall, its health care of competence exertion behind the palpus broken wall.Adopt the effective method broken wall can give full play to the effect of conidia powder.The glossy ganoderma spore powder with crushed sporoderm that this product is selected for use identifies that through Institute of Microorganism, Academia Sinica sporoderm-broken rate reaches more than 98%.
American Ginseng: be the dry root of the plant American Ginseng Panax quinquefolium L. of Araliaceae section.Nature and flavor: sweet, little hardship, cold; Function: boosting qi and nourishing yin, clearing heat and promoting fluid.The chemical composition of American Ginseng is mainly monose, polysaccharide, saponin(e, amino acid, protein, enzyme, organic acid, trace element etc.The major function of American Ginseng is " a beneficial lung yin, the clear fire of deficiency type is given birth to and soaked cough-relieving ".American ginseng extract is to the research emphasis of the immunity of organism regulating action immune pharmacological research at Radix Panacis Quinquefolii polysaccharide, American ginseng saponin and American Ginseng compound mixture.Radix Panacis Quinquefolii polysaccharide has the effect of increasing leukocyte, by promoting the spleen interstitial cell hyperplasia, performance compensatory extramedullar hematopoiesis function.American ginseng saponin can be anti-ageing, antiviral, antitumor and correct aspect such as acquired immunodeficiency and bring into play certain effect, can also be used for bone marrow suppression that chemotherapy causes and to the synergism and attenuation of chemotherapeutics.
Tiepi Fengdou, glossy ganoderma, American Ginseng are the rare traditional Chinese medicine that there is use history in several thousand in China, and continue to use always and do not wane, and " continue in the Chinese pharmacopoeia to list in wherein version in 2005.The treatment and the health-care effect of these medicinal materials have also been proved conclusively through modern pharmacology, medicine research and clinical practice.
The foundation of dendrobium officinale extracturm formulation selection of the present invention, be according to Chinese medical discrimination legislation and select the principle of medicine with method, at the generation development of hypoimmunity and the characteristics of performance, establishment is to help body healthy tendency, build up health, the anti-heresy of raising body, resistance against diseases are main principle, simultaneously in conjunction with modern bioscience achievement in research and the effect of Chinese medicine modern pharmacology, draft on the basis of Tiepi Fengdou.Compatibility glossy ganoderma spore powder with crushed sporoderm, American Ginseng play synergy to strengthen immunity.Wherein Tiepi Fengdou can reinforcing stomach reg fluid, nourishing Yin and clearing heat; American Ginseng benefit lung yin, the clear fire of deficiency type, the cough-relieving of promoting the production of body fluid; Glossy ganoderma spore powder with crushed sporoderm contains several physiological active substances.
Preferably, the raw material for preparing described dendrobium officinale extracturm is the mixture of Tiepi Fengdou, glossy ganoderma spore powder with crushed sporoderm and American Ginseng mass ratio 3:1:1.
Be concentrated into paste by Tiepi Fengdou, glossy ganoderma spore powder with crushed sporoderm and American Ginseng aqueous extract, can obtain described dendrobium officinale extracturm.Described aqueous extract can extract according to this area conventional method and obtain, and after can extracting respectively extract is mixed, and also can mix the back co-extracted, is concentrated into paste, promptly obtains described Tiepi Fengdou glossy ganoderma extract.
Among the present invention, described aqueous extract extracting method is as follows: Tiepi Fengdou, glossy ganoderma spore powder with crushed sporoderm and American Ginseng are independent separately or mix the back to add quality be the water of Tiepi Fengdou, glossy ganoderma spore powder with crushed sporoderm, American Ginseng or 8～12 times of quality of its mixture, being warming up to 90～98 ℃ extracted 1～3 time, each 1～3 hour, merge extract, obtain described Tiepi Fengdou, glossy ganoderma spore powder with crushed sporoderm and American Ginseng aqueous extract.Raw material of the present invention is Tiepi Fengdou, glossy ganoderma spore powder with crushed sporoderm and American Ginseng, all is rich in polysaccharose substance, and polysaccharide solubility in water is big, suitable water extracts, but the American Ginseng effective ingredient is a saponin(e, and saponin(e is water-soluble low, and conventional extracting method is to be that solvent carries out alcohol extracting with alcohol.Carry out water and carry and among the present invention American Ginseng mixed with Tiepi Fengdou, glossy ganoderma spore powder with crushed sporoderm, may have the hydrotropy effect, improved the extraction ratio of effective constituents in the American Ginseng to greatest extent, show through experimental study: saponin(e residual in the American Ginseng dregs of a decoction after mixing water is carried extracts more fully less than 5%.
Preferably, the raw material for preparing described dendrobium officinale extracturm is Tiepi Fengdou, glossy ganoderma spore powder with crushed sporoderm and the American Ginseng of mass ratio 3:1:1, and prepare described dendrobium officinale extracturm: with American Ginseng, Tiepi Fengdou and the glossy ganoderma spore powder with crushed sporoderm extractor of packing into by following method, adding quality is the water of 10～12 times of mixture qualities, slowly be warming up to little back insulation (90～98 ℃) insulation 1～3 hour of boiling, emit extract 1, filter residue 1 is stayed in the extractor; Adding quality in the extractor is the water of 8～10 times of filter residue 1 quality, is warming up to 90～98 ℃ of insulations 1～3 hour, emits extract 2, merges extract 1, extract 2, filters, and filtrate is concentrated into paste, obtains described dendrobium officinale extracturm.
It is raw material that the present invention adopts three kinds of valuable ingredients of traditional Chinese medicine, through extracting, concentrate the refining Tiepi Fengdou glossy ganoderma extract that forms, makes health-care efficacy more remarkable, and simultaneously, this product taking convenience is easy to preserve, and also is presenting friends and the elderly's desirable gift.
The production technology of Tiepi Fengdou glossy ganoderma extract of the present invention (clear cream), take the following step:
(1) raw material pre-treatment: carry out the preparatory processing of crude drug respectively.
(2) extract preceding the preparation: check instrument and equipment, check material rate.
(3) composition extracts: extract; Concentrate; Receive cream.
(4) packaging sterilizing: wash bottle; Bottling; Sterilization.
(5) warehouse-in: put into the warehouse after the inspection.
Tiepi Fengdou glossy ganoderma extract of the present invention through Nanjing public preventive medicine research institute test by " toxicology of food safety assessment process " regulation and turn out to be nontoxic level, it is safe taking for a long time.Through the health care evidence mouse humoral immune of enhancing power function is arranged, high dose can strengthen mouse macrophage phagocyte phagocytic function, can strengthen the effect of NK cytoactive, and effect is confirmed by scientific experiment.
According to " health food function assessment assessment process and the method for inspection standard " of Ministry of Public Health issue to enhancing immunity function method of inspection decision principle as a result, dendrobium officinale extracturm of the present invention has the effect of the immunity function of enhancing, can be used for preparing the health products that strengthen immunity.
Beneficial effect of the present invention is mainly reflected in: the dendrobium officinale extracturm that a kind of active constituent content height, effect are stable lasting, can strengthen immunity of organisms is provided, has been easy to carry, taking convenience, effect relax, and has the effect of better enhancing immunity of organisms.
(4) description of drawings
Fig. 1 is a Tiepi Fengdou glossy ganoderma extract process chart of the present invention.
(5) specific embodiment
The present invention is described further below in conjunction with specific embodiment, but protection scope of the present invention is not limited in this:
Embodiment 1:
The concrete factory formula of dendrobium officinale extracturm of the present invention (clear cream) is as shown in table 1:
Table 1
Above raw material is made cream 58.42kg clearly, and yield of extract is 29.31%.Make 437 bottles of the finished products of 130g/ bottle, yield rate is 97.33%.
According to shown in Figure 1, preparation technology of the present invention is as follows:
(1): the raw material pre-treatment:
Tiepi Fengdou: after cleaning, be crushed to 10 orders and feed intake.
Lucidum spore powder: after cleaning, 60 ℃ of vacuum drying are to containing moisture≤5%, put coldly, and put in the micronizer and pulverized about 1 hour, to conidia powder wall rate 〉=98%, should be airtight behind the broken wall, go into operation in 48 hours, or sealing,＜4 ℃ of preservations.
American Ginseng: after cleaning, remove dust with clear water flushing, again with the clear water spray to abundant suction deliquescing, slicer feeds intake after being cut into 3～5mm sheet, or≤put in storage after 65 ℃ of oven dry and deposit.
(2): prepare before extracting:
The raw material confirmation, quantity is consistent with prescription.
Water, electricity, the regular supply of vapour energy, record of production table etc. on deck.
Extraction, water for cleaning should be up to specification through check.
Extract, concentrate, equipment cleanings such as batching, filtration, can run well, various tool on deck.
(3): extraction process:
Extract: with Tiepi Fengdou, glossy ganoderma spore powder with crushed sporoderm and American Ginseng, the water that adds 2400kg after the mixing slowly is warming up to little back insulation (95～98 ℃) 3 hours of boiling, and emits extract, the water that adds 1600kg in the extractor is again emitted extract with the extraction of method heat after 3 hours.After extract merges, while hot through plate-frame filtering.
Concentrate: the extract after will filtering stops to concentrate after external circulation evaporator is concentrated into relative density about 1.15 (60～70 ℃).
Receive cream: will behind about 1/3rd capacity of concentrate inspiration scraper plate inspissator after external circulation evaporator concentrates, press the operation of scraper plate inspissator operational procedure, and regulate 60～65 ℃ of temperature, vacuum pact-0.08MPa (attention prevents bumping).Concentrate is constantly slowly added in the scraper plate concentration pan, makes evaporation capacity and addition keep the optimum state of balance.Being concentrated into relative density is about 1.3 (60～65 ℃).After stopping to receive cream, medicinal extract pipeline while hot is input to purifying area.
(4) packaging sterilizing:
Wash bottle: bottle and bottle cap are scrubbed three times to inside and outside cleaning with clear water, and the purifying pond is washed one time, puts upside down in dish; put in the baking oven together with bottle cap; 100 ℃ of baking 30min anhydrous mark or steam to the bottle take out and to put coldly, put in the closed container or add a cover and deposit after screwing.
Bottle sterilization: bottle and bottle cap were immersed in 75% ethanolic solution 10 minutes fully.After pass-through box entered clean area, taking-up was dried standby.
Bottling: will the Tiepi Fengdou glossy ganoderma extract through being up to the standards in purifying area quantitatively pack in the bottle, aluminium foil sealing is with bottle cap screwing.
Sterilization: the semi-finished product after will bottling are put in the sterilization tank, press the operation of sterilizer operational procedure, be warming up to pressure 0.1MPa gradually, 30 minutes (bottle) or 40 minutes (big bottle) is after the time, the steam off valve, pressure inside Yin Wendu descends and descends gradually, reduce to zero to pressure after, slowly open sterilization cover (attention prevents bottle explosion), take out, the bottle wall temperature should be slightly higher than room temperature.
External packing: label, adorn box, put specification; Seal mouthful label, vanning, joint sealing.
(5) warehouse-in:
Through control laboratory sampling (comprising the product that keep sample) after the assay was approved, fill out single warehouse-in.
The end product quality standard sees Table 2:
Table 2
Embodiment 2:
The concrete factory formula of dendrobium officinale extracturm of the present invention (clear cream) is as shown in table 3:
Table 3
Above raw material is made cream 38.95kg clearly, and yield of extract is 19.48%.Make 297 bottles of the finished products of 130g/ bottle, yield rate is 99.33%.
Preparation technology is with embodiment 1.The end product quality standard sees Table 4:
Table 4
Embodiment 3:
The concrete factory formula of dendrobium officinale extracturm of the present invention (clear cream) is as shown in table 5:
Table 5
Above raw material is made cream 48.68kg clearly, and yield of extract is 24.34%.Make 369 bottles of the finished products of 130g/ bottle, yield rate is 98.66%.
Preparation technology and quality standard are with embodiment 1.The end product quality standard is as shown in table 6:
Table 6
Embodiment 4:
The stability test of extract
1. batch number: the lot number of producing with embodiment 1 described method is that three batches of products of 20060915,20060918,20060922 are sample.
2. test unit: Zhejiang Academy of Medical Sciences
3. stability conditions and detect frequency: three batch samples are put in the stability test case, and 37 ℃～40 ℃ of temperature under relative humidity 70%～80% condition, were deposited three months, detect once totally four times in every month.
4. test basis: JJF1017, GB/T5009.2, GB/T5009.4, GB/T5009.11, GB/T5009.12, GB/T5009.17, GB/T4789.2, GB/T4789.3, GB/T4789.4, GB/T4789.5, GB/T4789.10, GB/T4789.11, GB/T4789.15, " health food check and assessment technique standard " 2003 editions, " health food functional component detection method ".
5. test item: net content minus deviation, relative density, ash content, lead, arsenic, mercury, total saponin(e, thick polysaccharide, total plate count, coliform, mould and saccharomycete, salmonella, Shigella, staphylococcus aureus, hemolytic streptococcus.
6. stability test the results are shown in Table 7:
7. stability test conclusion:
Through legal detection unit the tracking that three lot numbers carry out stable accelerated test process is detected, each month measured once, and three totally months, constant product quality can be preserved 24 months.
According to preceding method a plurality of batch products of embodiment 2 and embodiment 3 described methods productions are carried out stability test, consistent with above-mentioned conclusion.
Embodiment 5: the extract toxicological evaluation
1. batch number: the product with the lot number 20060915 of embodiment 1 described method preparation is a sample.
2. analytical unit: Nanjing Gong Wei Prevention Research institute.
3. detect and use animal: mouse, rat, male and female half and half.
4. detection foundation: Ministry of Public Health's " health food security toxicological evaluation program and method of inspection standard " 2003.
5. mensuration project:
5.1 acute toxicity test
5.2 genetic toxicity test
5.2.1 salmonella typhimurium/mammal microsomal enzyme test (Salmonella reversion test)
5.2.2 bone marrow cell micronucleus test
5.2.3 mouse sperm deformity test
5.3 30 days feeding trials
6. testing result and judgement
6.1 dendrobium officinale extracturm of the present invention concentrates female, the male its mouse oral LD of sample
50All greater than 21.5g/kgBW (being equivalent to former state 35.9g/kg BW), according to acute toxicity (LD50) dosage grade scale, this sample belongs to nontoxic level.
7.2 the negative experimental result of genetoxic experiment (Ames experiment, bone marrow cell micronucleus test, mouse sperm deformity test) shows, does not see that dendrobium officinale extracturm of the present invention concentrates sample the genetoxic effect is arranged.
6.3 rat 30d feeding trial is the result show, compare with control group, the ordinary circumstance of concentrated three dosage groups of the sample rat of dendrobium officinale extracturm of the present invention, body weight, food utilization, hematology, blood biochemical, organ weights, dirty body when high dose group histopathological examination there is no unusually.The concentrated sample high dose group dosage female, buck of dendrobium officinale extracturm wherein of the present invention is 120 times of human body recommended amounts (former state 100mg/kg BW/d).
Embodiment 6: the extract function assessment is estimated
1. batch number: the product with the lot number 20060915 of embodiment 1 described method preparation is a sample.
2. analytical unit: Nanjing Gong Wei Prevention Research institute.
3. detect and use animal: mouse, rat, male and female half and half.
4. detection foundation: Ministry of Public Health's " the health food security functions is learned assessment process and method of inspection standard " 2003.
5. mensuration project:
5.1 body weight, thymus gland/body weight ratio, spleen/body weight ratio
5.2 the mouse spleen lymphocyte conversion test (mtt assay) that ConA induces
5.3 dinitrofluorobenzene inducing mouse delayed allergy (ear swelling method)
5.4 antibody-producting cell detects (Jerne improves slide method)
5.5 serum hemolysin is measured (blood clotting method)
5.6 mouse carbon clearance test
5.7 Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell test (half intracorporal method)
5.8 the NK cytoactive is measured (determination of lactate dehydrogenase method)
6. testing result and judgement
6.1 the testing result of the body weight of animal used as test, thymus gland/body weight ratio and spleen/body weight ratio shows, under this experiment condition, the medicinal extract that tries when 500mg/kg BW/d, 1000mg/kg BW/d, 2000mg/kg BW/d (be equivalent to human body recommended intake 5 times, 10 times, 20 times) dosage, body weight, thymus gland/body weight ratio and the spleen/body weight ratio of animal are not seen obvious influence.
6.2 the mouse spleen lymphocyte conversion test result that ConA induces shows, under this experiment condition, the medicinal extract that tries when 2000mg/kgBW/d (be equivalent to human body recommended intake 20 times) dosage, have the effect that strengthens the mouse cell immunologic function.
6.3 dinitrofluorobenzene inducing mouse delayed allergy result of the test shows, under this experiment condition, the medicinal extract that tries when 2000mg/kg BW/d (be equivalent to human body recommended intake 20 times) dosage, have the effect that strengthens the mouse cell immunologic function.
Show 6.4 antibody-producting cell detects result of the test, under this experiment condition, institute's medicinal extract that tries when 2000mg/kg BW/d (be equivalent to human body recommended intake 20 times) dosage, the effect with enhancing mouse cell immunologic function.
6.5 the serum hemolysin test determination is the result show, under this experiment condition, the medicinal extract that tries when 2000mg/kg BW/d (be equivalent to human body recommended intake 20 times) dosage, have the effect that strengthens the mouse cell immunologic function.
6.6 mouse carbon clearance test result of the test shows, under this experiment condition, the medicinal extract that tries when 500mg/kg BW/d, 1000mg/kgBW/d, 2000mg/kg BW/d (be equivalent to human body recommended intake 5 times, 10 times, 20 times) dosage, mouse monokaryon phagocyte phagocytic function is not seen obvious influence.
Show 6.7 Turnover of Mouse Peritoneal Macrophages is engulfed the chicken red blood cell result of the test, under this experiment condition, the medicinal extract that tries when 2000mg/kgBW/d (be equivalent to human body recommended intake 20 times) dosage, have the effect that strengthens macrophage phagocytosis of mice.
6.8NK the cytoactive determination test shows, under this experiment condition, the medicinal extract that tries when 1000mg/kgBW/d, 2000mg/kgBW/d (be equivalent to human body recommended intake 10 times, 20 times) dosage, have the effect that strengthens the NK cytoactive.
According to strengthening the principle that immunity function method of inspection result judges in " the health food function assessment assessment process and the method for inspection standard " of Ministry of Public Health's issue, dendrobium officinale extracturm of the present invention has the effect of the immunity function of enhancing.
Claims (8)
1. dendrobium officinale extracturm, the raw material quality that it is characterized in that preparing described dendrobium officinale extracturm is composed as follows:
Tiepi Fengdou 10～70%
Glossy ganoderma spore powder with crushed sporoderm 10～60%
American Ginseng 10～60%;
Described glossy ganoderma spore powder with crushed sporoderm is that Reishi sporule obtains through breaking-wall cell.
2. dendrobium officinale extracturm as claimed in claim 1, the raw material that it is characterized in that preparing described dendrobium officinale extracturm is the mixture of Tiepi Fengdou, glossy ganoderma spore powder with crushed sporoderm and American Ginseng mass ratio 3:1:1.
3. the described dendrobium officinale extracturm of claim 1 is characterized in that: by Tiepi Fengdou, glossy ganoderma spore powder with crushed sporoderm and American Ginseng water extract the water extract, described water extract is concentrated into paste, obtain described dendrobium officinale extracturm.
4. dendrobium officinale extracturm as claimed in claim 3, it is characterized in that: Tiepi Fengdou, glossy ganoderma spore powder with crushed sporoderm and American Ginseng be independence or the back water that adds 8～12 times of quality of mixing separately, being warming up to 90～98 ℃ extracted 1～3 time, each 1～3 hour, merge extract, obtain described Tiepi Fengdou, glossy ganoderma spore powder with crushed sporoderm and American Ginseng aqueous extract.
5. dendrobium officinale extracturm as claimed in claim 3, the raw material that it is characterized in that preparing described dendrobium officinale extracturm is Tiepi Fengdou, glossy ganoderma spore powder with crushed sporoderm and the American Ginseng of mass ratio 3:1:1, and prepare described dendrobium officinale extracturm: with American Ginseng, Tiepi Fengdou and the glossy ganoderma spore powder with crushed sporoderm extractor of packing into by following method, adding quality is the water of 10～12 times of mixture qualities, slowly be warming up to little 90～98 ℃ of insulations in back 1～3 hour of boiling, emit extract 1, filter residue 1 is stayed in the extractor; Adding quality in the extractor is the water of 8～10 times of filter residue 1 quality, is warming up to 90～98 ℃ of insulations 1～3 hour, emits extract 2, merges extract 1, extract 2, filters, and filtrate is concentrated into paste, obtains described dendrobium officinale extracturm.
6. the preparation method of a dendrobium officinale extracturm, the raw material quality for preparing described dendrobium officinale extracturm is composed as follows: Tiepi Fengdou 10～70%, glossy ganoderma spore powder with crushed sporoderm 10～60%, American Ginseng 10～60%; Described glossy ganoderma spore powder with crushed sporoderm is that Reishi sporule obtains through breaking-wall cell; Described method is as follows: Tiepi Fengdou, glossy ganoderma spore powder with crushed sporoderm and American Ginseng be independence or the back water that adds 8～12 times of quality of mixing separately, being warming up to 90～98 ℃ extracted 1～3 time, each 1～3 hour, merge extract, obtain described Tiepi Fengdou, glossy ganoderma spore powder with crushed sporoderm and American Ginseng aqueous extract, described water extract is concentrated into paste, obtains described dendrobium officinale extracturm.
7. method as claimed in claim 6, it is characterized in that: the raw material for preparing described dendrobium officinale extracturm is Tiepi Fengdou, glossy ganoderma spore powder with crushed sporoderm and the American Ginseng of mass ratio 3:1:1, and prepare described dendrobium officinale extracturm: with American Ginseng, Tiepi Fengdou and the glossy ganoderma spore powder with crushed sporoderm extractor of packing into by following method, adding quality is the water of 10～12 times of mixture qualities, slowly be warming up to little 90～98 ℃ of insulations in back 1～3 hour of boiling, emit extract 1, filter residue 1 is stayed in the extractor; Adding quality in the extractor is the water of 8～10 times of filter residue 1 quality, is warming up to 90～98 ℃ of insulations 1～3 hour, emits extract 2, merges extract 1, extract 2, filters, and filtrate is concentrated into paste, obtains described dendrobium officinale extracturm.
8. as the application of the described dendrobium officinale extracturm of one of claim 1～5 in the health products of preparation raising immunity.
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| CN102670990A (en) * | 2012-05-14 | 2012-09-19 | 贵州省赤水市金钗石斛产业开发有限公司 | Method for preparing dendrobium nobile crystal |
| CN104277953A (en) * | 2013-07-04 | 2015-01-14 | 张征 | Preparation technology of Dendrobium officinale wine |
| CN104758611A (en) * | 2015-03-10 | 2015-07-08 | 济南骄泰信息技术有限公司 | Health-caring product enhancing immunity |
| CN108578583A (en) * | 2018-07-31 | 2018-09-28 | 杭州雪域生物技术有限公司 | A kind of China's hair spore bacteria composition and the preparation method and application thereof |
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| CN102670990A (en) * | 2012-05-14 | 2012-09-19 | 贵州省赤水市金钗石斛产业开发有限公司 | Method for preparing dendrobium nobile crystal |
| CN104277953A (en) * | 2013-07-04 | 2015-01-14 | 张征 | Preparation technology of Dendrobium officinale wine |
| CN104758611A (en) * | 2015-03-10 | 2015-07-08 | 济南骄泰信息技术有限公司 | Health-caring product enhancing immunity |
| CN108578583A (en) * | 2018-07-31 | 2018-09-28 | 杭州雪域生物技术有限公司 | A kind of China's hair spore bacteria composition and the preparation method and application thereof |
| CN108578583B (en) * | 2018-07-31 | 2021-07-27 | 杭州雪域生物技术有限公司 | Hirsutella sinensis composition and preparation method and application thereof |
| CN115607628A (en) * | 2022-10-20 | 2023-01-17 | 浙江长典药物技术开发有限公司 | Dendrobium candicum fluid extract, preparation process and equipment thereof |
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| CN101380103B (en) | 2010-12-22 |
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