CN101358977A - Applications of immune magnetic carbon microtubule for detecting hepatitis b surface antigen antibody - Google Patents
Applications of immune magnetic carbon microtubule for detecting hepatitis b surface antigen antibody Download PDFInfo
- Publication number
- CN101358977A CN101358977A CNA2008100717023A CN200810071702A CN101358977A CN 101358977 A CN101358977 A CN 101358977A CN A2008100717023 A CNA2008100717023 A CN A2008100717023A CN 200810071702 A CN200810071702 A CN 200810071702A CN 101358977 A CN101358977 A CN 101358977A
- Authority
- CN
- China
- Prior art keywords
- carbon
- hepatitis
- microtubule
- surface antigen
- immune
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Peptides Or Proteins (AREA)
Abstract
The present invention provides an application of the immunomagnetic carbon tubulin in the detection of the hepatitis B surface antigen antibody, and relates to an immunomagnetic carbon tubulin, in particular to an application of the immunomagnetic carbon tubulin in the detection of the hepatitis B surface antigen antibody. The invention provides the application of the immunomagnetic carbon tubulin in the detection of the hepatitis B surface antigen antibody. The immunomagnetic carbon tubulin consists of a ferroferric oxide core and an outer layer; the outer layer is coated outside the core and comprises a carbon shell layer; the carbon shell layer is connected with amino. The application of the immunomagnetic carbon tubulin in the detection of the hepatitis B surface antigen antibody comprises the activation of the immunomagnetic carbon tubulin, the chemical covalent coupling of the immune ligand, the closing, and the immune detection of the magnetic carbon tubulin of the coupled hepatitis B surface monoclonal antibodies in the sandwich method.
Description
Technical field
The present invention relates to a kind of immune magnetic carbon microtubule, especially relate to the application of a kind of immune magnetic carbon microtubule in detecting hepatitis b surface antigen antibody.
Background technology
The chemical stability of material with carbon element is very high, these characteristics make it have wide practical use in catalysis and biomedicine field, but carbon nano-tube has very high chemical inertness, makes it all have application prospect in fields such as physics, chemistry, material, machinery, aviation, military affairs and biological medicines.The immune magnetic particle is the new technology that development in recent years is got up, and is used for separating concentrating waiting in the test more, and magnetic particle can combine with covalent manner with protein and not influence its activity, so be commonly used to catch specific protein or protein isolate.Immunomagnetic isolation is based on the magnetic particle new developing technology, by antibody being fixed on the magnetic particle carrier, realizes the step separation and purification to target antigen, having magnetic response efficiently simultaneously, has the specificity of height again.General methods for immobilizing antibodies has physisorphtion and chemical coupling method, and the former joint efficiency is low and have an antibody leakage problem.The latter's chemical functional group's chemical coupling method exists sensitivity low and space bit is resistive.
(Xiaofeng Wang et al.Adv.Funct.Mater.2008 such as nearest Xiaofeng Wang, 18,1809-1823) reported magnetic carbon microtubule preparation methods, prepared material surface has the carbon micro-/ nano tubular construction of amido functional group, wherein carbon pipe inside has also been filled and has been had ferromagnetic ferriferrous oxide nano/micro wire, has formed nucleocapsid structure.
Summary of the invention
The object of the present invention is to provide the application of a kind of immune magnetic carbon microtubule in detecting hepatitis b surface antigen antibody.
Immune magnetic carbon microtubule of the present invention is by tri-iron tetroxide core and outer the composition, and skin wraps up core, and skin is the carbon shell, is connected with amino on the carbon shell.Described core preferably diameter is the tri-iron tetroxide core of 10nm～20 μ m, and the carbon in the described carbon shell is the potpourri of graphitic carbon and agraphitic carbon, presses mass ratio, and the ratio of graphitic carbon and agraphitic carbon is 1: 1.
The preparation method of immune magnetic carbon microtubule of the present invention may further comprise the steps:
1) with iron salt dissolved in solvent, iron salt solutions;
2) halogeno-benzene is dissolved in benzene kind solvent, gets halogeno-benzene solution;
3) the pH value with alkali adjusting iron salt solutions is 8～11, mixes with halogeno-benzene solution again, gets mixed solution, and the mol ratio of molysite and halogeno-benzene is 1: (0.3～3);
4) mixed solution is poured in the reactor reacted, after the cooling solution in the reactor is poured out, centrifugal collection insolubles, washing, drying obtains the amidized magnetic carbon tube magnetic carbon tube carbon micro-/ nano pipe of amino functional (or be called).
The concentration of iron salt solutions is 0.2%～4% by mass percentage; Described molysite is preferably the derivant of ferrocene and ferrocene; The derivant of ferrocene is selected from tert-butyl group ferrocene, N, N-methyl aminomethyl ferrocene, methylol ferrocene, formic acid ferrocene, formaldehyde ferrocene, acetonitrile ferrocene, 1,1 '-ferrocene dicarboxylic acid, 1,1 '-ferrocene dicarbaldehyde, 1,1 '-dibromo ferrocene, diamido ferrocene, (+/-)-dimethyl-1-ferrocene ethamine or+/-) 1-ferrocenyl ethanol etc.; Described solvent is D, and D-dimethyl formamide (DMF) or lower alcohol, described lower alcohol are at least a in methyl alcohol, ethanol, propyl alcohol, the low molecular poly etc.The concentration of halogeno-benzene solution is 0.13%～4% halogeno-benzene solution by mass percentage; Benzene kind solvent is at least a in benzene, toluene, ethylbenzene, the dimethylbenzene etc.; Halogeno-benzene is selected from phenyl-hexabromide, tribromo-benzene, hexachlorobenzene, trichlorophenol, trichlorobenzoic acid or trichloro-benzoyl chloride etc.; Alkali is ammoniacal liquor, ethamine, ethylenediamine or polyethylene polyamine etc.
Pour mixed solution into react in the reactor temperature and be preferably 180～250 ℃, the time of reaction is preferably 1～72h.
Product characterizes through transmission electron microscope, scanning electron microscope, and the diameter of the amidized magnetic carbon tube of the gained magnetic carbon tube carbon micro-/ nano pipe of amino functional (or be called) is 10nm～20 μ m, and length is 100～1mm.Characterize and contrast through X-ray diffraction, confirm that product is mainly the tri-iron tetroxide that carbon coats with standard database; Confirm mutually through X-ray diffraction photoelectron spectroscopy sign and Raman spectrum data, show that the peripheral carbon shell of product mainly is graphitic carbon and part agraphitic carbon; Characterizing inner core through high-resolution-ration transmission electric-lens is tri-iron tetroxide, and skin has the nucleocapsid structure of carbon coated iron oxide; X-ray diffraction photoelectron spectral data and ir data confirm that carbon shell surface has amino.
Utilize fluorescein isothiocynate (FITC) and amino reaction to obtain stable product, observe fluorescently-labeled carbon micron tube at laser copolymerization microscopically.Aspect electrochemical properties, at first add 0.1～1mL red fuming nitric acid (RFNA) among the alcohol dispersion liquid 10mL of micrometer/nanometer tubulose carbon structure magnetic material with the 2mg amino functional of preparation, in 20～80 ℃ of water-baths, heat 5min～72h, the tri-iron tetroxide of removing parcel in the micron tube with the carbon microtubule material that obtains hollow amino functional (hollowcarbon microtubes, H-CMTs).Experimental selection dopamine (DA) and ascorbic acid (AA) as the research thing, study their oxidation-reduction behaviors on solid carbon microtubule and hollow carbon microtubule.
The application of immune magnetic carbon microtubule of the present invention in detecting hepatitis b surface antigen antibody may further comprise the steps:
1) activation of immune magnetic carbon microtubule: the immune magnetic carbon microtubule that primary amino radical group is contained on the surface is immersed in the glutaraldehyde solution and reacts, and through magnetic resolution and washing, obtains the immune magnetic microtubule that aldehyde radical is contained on the surface;
2) chemical covalent coupling immunity aglucon: the immune magnetic microtubule of aldehyde radical is contained on the surface that step 1) is obtained and the solution of immune aglucon mixes, and reacts in shaking table, through magnetic resolution and washing, obtains the immune magnetic carbon microtubule of surperficial coupling immunity aglucon;
3) sealing: add the excessive aldehyde radical of lysine sealing, add bovine serum albumin(BSA) and prevent the antigen-antibody non-specific adsorption of dissociating, reaction, magnetic separates also washing, is resuspended in the 10mM phosphate buffer of 10%BSA;
4) the sandwich method immune detection detects the magnetic carbon microtubule of coupling hepatitis B surface monoclonal antibody: each of 96 hole flat boards adds variable concentrations same volume hepatitis B virus surface antigen solution and negative controls respectively as in the hole of enzyme immunoassay (EIA), the magnetic carbon microtubule reaction that adds the coupling hepatitis B surface monoclonal antibody that obtains in the step 3), the back adds the hepatitis B virus surface antigen reaction of horseradish peroxidase mark, through magnetic resolution, with the phosphate buffer solution washing that contains polysorbas20, supernatant discarded, the substrate solution that in each hole, adds 100 μ l then, after 37 ℃ dark place colour developing, in each hole, add reaction terminating liquid, measure absorbance, detect hepatitis b surface antigen antibody thus.
In step 1), number percent by volume, the concentration of glutaraldehyde solution is 0.2%～50%, and the temperature of reaction is preferably 25～35 ℃, and the time of reaction is preferably 6～18h, and washing can be adopted the carbonic acid buffer washing.
In step 2) in, described immune aglucon is the hepatitis B surface monoclonal antibody.
In step 3), number percent by volume, the aldehyde radical that the lysine sealing is excessive and the ultimate density of bovine serum albumin(BSA) are 1%, and the reaction time is 1h.
In step 4), the time that adds the magnetic carbon microtubule reaction of the coupling hepatitis B surface monoclonal antibody that obtains in the step 3) is preferably 30min, and the time that the back adds the hepatitis B virus surface antigen reaction of horseradish peroxidase mark is preferably 30min; Through magnetic resolution, wash best 3～5 times with the phosphate buffer solution that contains polysorbas20; It is the tetramethyl benzidine of 100 μ l/ml that substrate solution can adopt volume ratio, and percent by volume concentration is 0.006% hydrogen peroxide, and pH is 4.5 citric acid phosphoric acid salt buffer; Time 37 ℃ dark place colour developings is preferably 15min; It is the sulfuric acid of 1M that reaction terminating liquid can adopt concentration; Measuring absorbance can adopt microplate reader to measure absorbance under 450nm.
Compare with the immune magnetic particulate of prior art, immune magnetic carbon microtubule of the present invention has the following advantages:
1) diameter of microtubule is 10～2000nm, and length is 0.01～0.1mm, so this microtubule has big specific surface area, steric hindrance is little, is convenient to the more function aglucon of coupling; 2) amino on microtubule surface is connected on the carbon shell of height chemical stability and helps immune detection and background signal is low, has higher sensitivity; 3) microtubule contains quantity of magnetism height, and very strong magnetic responsiveness is arranged, and can realize the quick separation in conventional magnetic field.4) this microtubule is the micron/sub-micron tubulose carbon structure magnetic material of amino functional, and material is even, purity is high, monodispersed core-shell structural, traditional relatively method, and equipment is relatively inexpensive, and temperature is low, can keep the functional group on reactive material surface.
It is resistive that the present invention adopts the chemical coupling be shaped as tubulose largely to overcome the space bit of traditional microballoon; The employing core is the advantage of the ferromagnetism surface chemistry inertia of tri-iron tetroxide, outer carbon shell, compares traditional polymer microsphere and greatly reduces non-specific adsorption, thereby reduced the body signal, realizes high sensitivity.Immune magnetic carbon microtubule of the present invention is applied to can distinguish 0.1ng/ml and negative sample in the hepatitis b surface antigen antibody detection.
Description of drawings
Fig. 1 is the sem photograph of the product of the embodiment of the invention 1 preparation.
Embodiment
The invention will be further described in conjunction with the accompanying drawings below by embodiment.
Embodiment 1: the magnetic carbon microtubule of preparation primary amino radical group
At ambient temperature, ferrocene 20mg is dissolved in 10ml D, D-dimethyl formamide (DMF), the formation mass percent is 0.2% solution; Phenyl-hexabromide 28mg is dissolved in 10ml toluene, and the formation mass percent is 0.28% solution.Above-mentioned two kinds of solution are mixed, and adding ammoniacal liquor 1ml pH value is 8～11, mixes.Pour mixed solution into the teflon reactor, be enclosed within the stainless steel cauldron.With reactor at 250 ℃ and insulation reaction 24h.After the cooling solution in the reactor is poured out, centrifugal collection insolubles, and with ethanol washing at least 3 times, drying obtains the micron/sub-micron tubulose carbon structure magnetic material (Me-CMTs) (referring to Fig. 1) of the amino functional of 0.1mm length.
Embodiment 2: use the magnetic carbon microtubule of primary amino radical group to prepare immune magnetic carbon microtubule of the present invention
The activation of immune magnetic carbon microtubule: the immune magnetic carbon microtubule immersion 0.05mol/L of primary amino radical group is contained on the surface that 1.0mg embodiment 1 obtains, pH=9.6 carbonic acid buffer 1ml, adding 20 μ l contains in 25v% (volume by volume concentration) glutaraldehyde, room temperature reaction 12h, through magnetic resolution and with the carbonic acid buffer washing, obtain the immune magnetic microtubule that aldehyde radical is contained on the surface.
Chemistry covalent coupling immunity aglucon: this immune magnetic microtubule that contains aldehyde radical in the centrifuge tube of 1.5ml, is added 2 μ l and contains 9.7mg/ml hepatitis B surface monoclonal antibody, and concussion reaction 4h is scattered in the 10mM phosphate buffer of 10%BSA in 30 ℃ of shaking tables.
Embodiment 3: the sandwich method immune detection detects the magnetic carbon microtubule of coupling hepatitis B surface monoclonal antibody
Add 40 μ l hepatitis B surface monoclonal anti original solutions in each of 96 hole flat boards (Ying Kexin wound Xiamen company limited produce) respectively as in the hole of enzyme immunoassay (EIA), concentration is respectively standard quality-control product 4ng/ml, 3ng/ml, 2ng/ml, 1ng/ml, 0.5ng/ml, 0.2ng/ml, 0.1ng/ml (back all with obtain behind the enzyme diluted 5ng/ml); And add a negative control sample.It is 1,2,3,4,5,6,7,8 that numbering is respectively, and adds the magnetic carbon microtubule reaction 30min of the coupling hepatitis B surface monoclonal antibody that obtains among the embodiment 2, and the back adds hepatitis B virus surface antigen (second antigen) the reaction 30min of horseradish peroxidase mark.Through magnetic resolution, with the phosphate buffer solution washing that contains polysorbas20 3～5 times, supernatant discarded.Substrate solution (the tetramethyl benzidine of 100 μ l/ml that in each hole, adds 100 μ l then, 0.006% hydrogen peroxide, the citric acid phosphoric acid salt buffer of pH4.5), behind 37 ℃ dark place colour developing 15min, in each hole, add the reaction terminating liquid (sulfuric acid of 1M) of 100 μ l.Dual wavelength microplate reader (Denley dragon MK2) is measured absorbance under 450/630nm, the result is referring to table 1.The method of this invention of data presentation can be distinguished 0.1ng/ml in the table, and the general clinical 5ng/ml that only requires, so it has very high sensitivity, background signal is low.
Table 1
| The standard quality-control product | 5ng/ml | 4ng/ml | 3ng/ml | 2ng/ml | 1ng/ml | 0.5ng/ml | 0.1ng/ml | Negative control |
| Absorbance | 1.792 | 0.901 | 0.701 | 0.628 | 0.448 | 0.312 | 0.203 | 0.03 |
Claims (10)
1. the application of immune magnetic carbon microtubule in detecting hepatitis b surface antigen antibody, described immune magnetic carbon microtubule is by tri-iron tetroxide core and outer the composition, and skin wraps up core, and skin is the carbon shell, is connected with amino on the carbon shell.
2. the application of immune magnetic carbon microtubule as claimed in claim 1 in detecting hepatitis b surface antigen antibody, it is characterized in that described core is that diameter is the tri-iron tetroxide core of 10nm～20 μ m, the carbon in the described carbon shell is the potpourri of graphitic carbon and agraphitic carbon.
3. the application of immune magnetic carbon microtubule as claimed in claim 2 in detecting hepatitis b surface antigen antibody is characterized in that by mass ratio the ratio of graphitic carbon and agraphitic carbon is 1: 1.
4. the application of immune magnetic carbon microtubule as claimed in claim 1 in detecting hepatitis b surface antigen antibody is characterized in that may further comprise the steps:
1) activation of immune magnetic carbon microtubule: the immune magnetic carbon microtubule that primary amino radical group is contained on the surface is immersed in the glutaraldehyde solution and reacts, and through magnetic resolution and washing, obtains the immune magnetic microtubule that aldehyde radical is contained on the surface;
2) chemical covalent coupling immunity aglucon: the immune magnetic microtubule of aldehyde radical is contained on the surface that obtains in the step 1) and the solution of immune aglucon mixes, in shaking table, react,, obtain the immune magnetic carbon microtubule of surperficial coupling immunity aglucon through magnetic resolution and washing;
3) sealing: add the excessive aldehyde radical of lysine sealing, add bovine serum albumin(BSA) and prevent the antigen-antibody non-specific adsorption of dissociating, reaction, magnetic separates also washing, is resuspended in the 10mM phosphate buffer of 10%BSA;
4) the sandwich method immune detection detects the magnetic carbon microtubule of coupling hepatitis B surface monoclonal antibody: each of 96 hole flat boards adds variable concentrations same volume hepatitis B virus surface antigen solution and negative controls respectively as in the hole of enzyme immunoassay (EIA), the magnetic carbon microtubule reaction that adds the coupling hepatitis B surface monoclonal antibody that obtains in the step 3), the back adds the hepatitis B virus surface antigen reaction of horseradish peroxidase mark, through magnetic resolution, with the phosphate buffer solution washing that contains polysorbas20, supernatant discarded, the substrate solution that in each hole, adds 100 μ l then, after 37 ℃ dark place colour developing, in each hole, add reaction terminating liquid, measure absorbance, detect hepatitis b surface antigen antibody thus.
5. the application of immune magnetic carbon microtubule as claimed in claim 4 in detecting hepatitis b surface antigen antibody, it is characterized in that in step 1), number percent by volume, the concentration of glutaraldehyde solution is 0.2%～50%, the temperature of reaction is 25～35 ℃, the time of reaction is 6～18h, and the carbonic acid buffer washing is adopted in washing.
6. the application of immune magnetic carbon microtubule as claimed in claim 4 in detecting hepatitis b surface antigen antibody is characterized in that in step 2) in, described immune aglucon is the hepatitis B surface monoclonal antibody.
7. the application of immune magnetic carbon microtubule as claimed in claim 4 in detecting hepatitis b surface antigen antibody, it is characterized in that in step 3), number percent by volume, the aldehyde radical that the lysine sealing is excessive and the ultimate density of bovine serum albumin(BSA) are 1%, and the reaction time is 1h.
8. the application of immune magnetic carbon microtubule as claimed in claim 4 in detecting hepatitis b surface antigen antibody, it is characterized in that in step 4), the time that adds the magnetic carbon microtubule reaction of the coupling hepatitis B surface monoclonal antibody that obtains in the step 3) is 30min, and the time that the back adds the hepatitis B virus surface antigen reaction of horseradish peroxidase mark is 30min; Through magnetic resolution, with the phosphate buffer solution washing that contains polysorbas20 3～5 times.
9. the application of immune magnetic carbon microtubule as claimed in claim 4 in detecting hepatitis b surface antigen antibody, it is characterized in that in step 4), it is the tetramethyl benzidine of 100 μ l/ml that substrate solution adopts volume ratio, percent by volume concentration is 0.006% hydrogen peroxide, and pH is 4.5 citric acid phosphoric acid salt buffer.
10. the application of immune magnetic carbon microtubule as claimed in claim 4 in detecting hepatitis b surface antigen antibody is characterized in that in step 4), is 15min in the time of 37 ℃ dark place colour developings; It is the sulfuric acid of 1M that reaction terminating liquid adopts concentration; Measuring absorbance adopts microplate reader to measure absorbance under 450nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100717023A CN101358977A (en) | 2008-09-02 | 2008-09-02 | Applications of immune magnetic carbon microtubule for detecting hepatitis b surface antigen antibody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100717023A CN101358977A (en) | 2008-09-02 | 2008-09-02 | Applications of immune magnetic carbon microtubule for detecting hepatitis b surface antigen antibody |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101358977A true CN101358977A (en) | 2009-02-04 |
Family
ID=40331480
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008100717023A Pending CN101358977A (en) | 2008-09-02 | 2008-09-02 | Applications of immune magnetic carbon microtubule for detecting hepatitis b surface antigen antibody |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101358977A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102967706A (en) * | 2012-11-21 | 2013-03-13 | 济南大学 | Preparation method and application of flow injection chemiluminiscence immuno sensor for detecting tumor marker |
-
2008
- 2008-09-02 CN CNA2008100717023A patent/CN101358977A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102967706A (en) * | 2012-11-21 | 2013-03-13 | 济南大学 | Preparation method and application of flow injection chemiluminiscence immuno sensor for detecting tumor marker |
| CN102967706B (en) * | 2012-11-21 | 2014-09-03 | 济南大学 | Preparation method and application of flow injection chemiluminiscence immuno sensor for detecting tumor marker |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Tang et al. | A review on advances in methods for modification of paper supports for use in point-of-care testing | |
| Zhang et al. | Recent advances in the construction of functionalized covalent organic frameworks and their applications to sensing | |
| CN105403697B (en) | It is a kind of microsphere supported and preparation method thereof | |
| CN107478837B (en) | Micro-fluidic chemiluminescence detection system and its application based on magnetic particle | |
| JPS61292059A (en) | Solid phase system for ligand-receptor-assay | |
| CN101692023B (en) | Chemical preparing method of molecular recognition of ultratrace explosive TNT based on fluorescence resonance energy transfer | |
| Yan et al. | Interfacial assembly of signal amplified multienzymes and biorecognized antibody into proteinosome for an ultrasensitive immunoassay | |
| WO2010061992A1 (en) | Method for amplification of signal in immunochromatographic assay and immunochromatographic kit using the method | |
| Zhang et al. | Application trends of nanofibers in analytical chemistry | |
| CN103278637B (en) | A kind of carbon nano-tube Test paper detecting helicobacter pylori and preparation method thereof | |
| Xiao et al. | Fluorescent nanomaterials combined with molecular imprinting polymer: synthesis, analytical applications, and challenges | |
| CN101225306B (en) | Method for preparing fluorescent rare earth complex compound silicon nano particles | |
| CN104634973B (en) | A kind of preparation method and application of nm of gold composite immunosensor | |
| CN102608189B (en) | Method for manufacturing nanometer magnetic ferroferric oxide modified immunosensor | |
| Mahmoudifard et al. | Electrospun polyethersolfone nanofibrous membrane as novel platform for protein immobilization in microfluidic systems | |
| Liang et al. | Conductometric immunoassay of alpha-fetoprotein in sera of liver cancer patients using bienzyme-functionalized nanometer-sized silica beads | |
| CN102621321A (en) | Construction method of sensitive interface of immunosensor for detecting pesticide residues | |
| KR20190070726A (en) | Magnetic nanoparticles and method for amplification of signal in lateral flow assay by using the same | |
| US20160341723A1 (en) | Au nanoparticles encapsulated in nanocompoites and applications thereof in rapid detection of an analyte | |
| Chen et al. | Recent progress in the application of microfluidic systems and gold nanoparticles in immunoassays | |
| CN101358977A (en) | Applications of immune magnetic carbon microtubule for detecting hepatitis b surface antigen antibody | |
| CN112730338A (en) | Ag @ Au-based porous structure dual-signal nano amplification probe and SPR immunodetection method thereof | |
| Ghafary et al. | Ultrasensitive fluorescence immunosensor based on mesoporous silica and magnetic nanoparticles: Capture and release strategy | |
| WO2018194151A1 (en) | Method for detecting specimen substance using multiphase polymer fine particles | |
| CN201917575U (en) | Nano influenza virus detection test paper |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20090204 |