CN101358974A - Prostatic cancer early diagnosis kit based on ZnO nano-bar array - Google Patents
Prostatic cancer early diagnosis kit based on ZnO nano-bar array Download PDFInfo
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- CN101358974A CN101358974A CNA2007100496596A CN200710049659A CN101358974A CN 101358974 A CN101358974 A CN 101358974A CN A2007100496596 A CNA2007100496596 A CN A2007100496596A CN 200710049659 A CN200710049659 A CN 200710049659A CN 101358974 A CN101358974 A CN 101358974A
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Abstract
The present invention provides a ZnO nanorod array-based kit which is used for the early diagnosis of the prostate cancer. The kit adopts prostate specific antibodies of the prostate protein labeling material. The kit comprises an improved ZnO nanorod array film which is coated by the prostate specific antibodies, a bottom plate with absorbent paper, a micro field device (with an electrophoresis tank and a pair of electrodes) and buffer solution; the invention also relates to the utilizing and detecting method of the kit. The kit can be used for the diagnosis and mass screening of benign and malignant prostate tumors, in particular for the detection of the clinical cancer of the prostate and the detection of the incidental carcinoma of the prostate. The invention has the characteristics of high speed, sensitivity, accuracy, reliability, simple operation, and suitability for the primary health care units.
Description
Technical field
The present invention relates to biomedical detection range, particularly adopt tumor-marker antibody modification ZnO nano-stick array thin film to detect the early diagnosis and the generaI investigation of prostate cancer.
Background technology
Prostate cancer is the male reproductive system common malignancy.The incidence of disease of prostate cancer has very big difference at home and abroad.The European and American countries incidence of disease is higher, and the east incidence of disease is lower.But, in recent years, the trend of rising was arranged at the incidence of disease of China.According to estimates, in the middle of the male sex more than 50 years old, all suffers from the very cancer of small size in nearly 40% the human prostate; There is 8% the male sex to develop into noticeable prostate cancer disease clinically, has 3% the male sex will die from this disease.In most of cases, prostate cancer is slower development in the older male sex.Therefore all find in the diagnosis of the prostate cancer most of prostate cancers of American-European countries occurred frequently by the cancer generaI investigation.When patient itself found the clinical manifestation of prostate cancer is arranged, the state of an illness had arrived middle and advanced stage usually.Experiment showed, patient more than half, the transfer of prostate cancer will take place in 1 year after subjective symptoms occurs.The best means of finding prostate cancer early is the check by annual digital rectal examination and PSA (prostate specific antigen) index, and these two checks all are necessary inspection means in the prostate generaI investigation.Owing to do not carry out the generaI investigation of prostate cancer, so we must have the various symptoms of prostate cancer, and a sufficient understanding just can accomplish early to find, early treatment in most of Asian countries.
Prostate specific antigen (PSA) has been widely used in clinical, becomes one of important means of early diagnosis and screening prostate cancer.PSA is a kind of enzyme that is produced by the prostate epithelial cell endochylema, just often in the blood content seldom, in case suffer from prostate cancer, PSA just can enter blood in a large number.Measure the PSA content in the blood, just know and whether suffered from prostate cancer, and the state of an illness is more serious, the PSA value is high more.In addition, by mensuration, can determine sensitively also whether some tumor tissues derives from prostate, and its accuracy can reach 100% to PSA.
Because have rapidly and efficiently, in enormous quantities, characteristics such as high sensitivity, selectivity, the ZnO nano-stick array thin film of biomolecule modification is widely used in many analyses such as protein component and function, and new drug development and medical diagnosis on disease etc.Adopt the ZnO nano-stick array thin film, can firmly be connected with biomolecule such as albumen, luminous wave spectrum is narrow, luminous intensity is high, these characteristics make this film have more Practical significance widely, and are used for that biological information detects and medical diagnosis on disease is analyzed.It has become after fluorescein, enzyme labeling nanogold particle, silicon nanowires, and a kind of on-radiation comparatively advanced and commonly used detects the mark agent.It is compared simply, fast than detections tumour methods such as radiommunoassay, EIA enzyme immunoassay, fluorescence analysis and chemo-immunity analysis at present commonly used, only needs to finish in about ten minutes, and is less demanding to operating personnel, easy grasp.
Summary of the invention
The purpose of this invention is to provide a kind of prostatic cancer early diagnosis kit based on the ZnO nanometer stick array, it can fast and have characteristics such as highly sensitive, that specificity good, testing result is stable, thereby can promptly finish the detection method and the kit of prostate cancer marker from the fresh blood sample to the output result.
Technical scheme of the present invention is as follows:
Comprise base plate, electric microfield device (attached electrophoresis tank, to electrode) and damping fluid based on the prostatic cancer early diagnosis kit of ZnO nanometer stick array.Wherein the base plate lower end is coated with the ZnO nano-stick array thin film, and the upper end of base plate is coated with adsorptive pads.Described ZnO nano-stick array thin film is connected with the antibody protein that can catch one or both prostate specific antigen.When connecting two kinds of antibody proteins, be divided into two zones up and down: go up the district and be that the ZnO nano-stick array thin film of total prostate specific antibody (anti-T-PSA) modification, inferior segment are the ZnO nano-stick array thin film of free prostate gland specificity antibody (anti-F-PSA) modification.The electric microfield device that this kit also comprises (with electrophoresis tank, to electrode) provides in the fast enriching sample specific antigen to the device on ZnO nanometer stick array surface.Wherein electrophoresis tank is 2 * 1 * 2cm
3The organic glass liquid holding groove, power supply is provided by the micro spectral detecting device.
The step of the method for employing ZnO nano-stick array thin film detection prostate cancer is as follows:
(1) fresh serum with 1-2ml tester joins in the electrophoresis tank, and is diluted to 3-4ml with damping fluid
(2) the careful insertion detected base plate in sample solution, and liquid level rolls off the production line with base plate thieving paper and flushes, and installs electric microfield then, regulates electrode direction and energized simultaneously, and electric microfield voltage is 0.1-50V;
(3) powered-down after 1-20 minute carefully takes out detected base plate, and hot blast drying is used at last to remove the non-tumor antigen protein of unnecessary absorption in the ZnO array detection zone of laterally washing base plate with damping fluid then.
(4) put in the spectral investigator detecting base plate, carry out spectral detection.
(5) the baseplate irradiated intensity of measuring is compared with the photoluminescence intensity of standard, thus in definite blood sample to be measured prostate specific antigen whether exist and/quantity.
The invention has the beneficial effects as follows, protein biology molecule in bio-modification ZnO nano-stick array thin film surface can enriched sample solution under by the effect of electric microfield, and being combined in formation biologic array on the ZnO nanometer rods, this biologic array can strengthen the ZnO photoluminescence intensity; The percentage that increases according to the luminous intensity of array after the electric field enrichment is judged the concentration effect of albumen.
The enrichment associated methods of this albumen lays the foundation in the application of biological information detection and biology sensor for realizing the ZnO nano-stick array thin film.
Description of drawings
Fig. 1: pass through protein molecular synoptic diagram in the electric microfield enrichment lean solution with the ZnO nanometer stick array.
Fig. 2: kit base arrangement synoptic diagram.
Embodiment
Below in conjunction with embodiment the present invention is elaborated, but therefore do not limit the present invention within the described scope of embodiments.
(1) 2.0ml fresh serum sample is joined in the electrophoresis tank, and be diluted to 3.5ml with damping fluid;
(2) the careful insertion detected base plate in sample solution, and liquid level rolls off the production line with base plate thieving paper and flushes, and installs electric microfield then; Regulating electrode direction, make and detect base plate connection positive source, is power cathode to electrode, and energized;
(3) powered-down after 7-10 minute carefully takes out detected base plate, and hot blast drying is used at last to remove the non-tumor antigen protein of unnecessary absorption in the ZnO array detection zone three times of laterally washing base plate with damping fluid then.
(4) put into the micro spectral tester detecting base plate, carry out spectral detection.Wherein excitation wavelength is 260nm, detects the emission light wave of wavelength coverage at 290-380nm.
Specific embodiment of the invention result sees table 1 for details.
The content of detected target antigen among table 1 embodiment 1,2
The present invention compares with existing detection technique of fluorescence, and the main distinction is that the ZnO nano-rod array film is as detecting substrate Have the excitation wave length and width, the characteristics such as emission spectrum is narrow, luminous intensity is high, no photobleaching, thereby in detection, have and be swift in response The characteristics good with sensitivity.
Claims (9)
1. the prostatic cancer early diagnosis kit based on the ZnO nanometer stick array is characterized in that it comprises base plate, is provided with thieving paper in the base plate upper end, is fixed with prostate specific antibody modification ZnO nano-stick array thin film in the base plate lower end.Wherein array film is divided into two zones up and down: go up the district and be that the ZnO nano-stick array thin film of total prostate specific antibody (anti-T-PSA) modification, inferior segment are the modification ZnO nano-stick array thin film of free prostate gland specificity antibody (anti-F-PSA).
2. the prostatic cancer early diagnosis kit based on the ZnO nanometer stick array according to claim 1 is characterized in that, the composition of this kit also comprises drying agent, electric microfield device (with electrophoresis tank, to electrode) and damping fluid.
3. the prostatic cancer early diagnosis kit based on the ZnO nanometer stick array according to claim 2, it is characterized in that the included electric microfield device of this kit (with electrophoresis tank, to electrode) provides in the fast enriching sample solution specific antigen to the device on ZnO nanometer stick array surface.Wherein electrophoresis tank is 2 * 1 * 2cm
3The organic glass liquid holding groove, electric microfield voltage is 0.1-100V, power supply is provided by the micro spectral detecting device.
4. the prostatic cancer early diagnosis kit based on the ZnO nanometer stick array is characterized in that, the detection method of kit may further comprise the steps:
(1) sample solution with 1-2ml tester joins in the electrophoresis tank, and is diluted to 3-4ml with damping fluid;
(2) the careful insertion detected base plate in sample solution, and liquid level rolls off the production line with base plate thieving paper and flushes, and installs electric microfield then, regulates electrode direction and energized simultaneously;
(3) powered-down after 1-20 minute carefully takes out detected base plate, laterally washes the ZnO array detection zone of base plate then with damping fluid, to remove the non-tumor antigen protein of unnecessary absorption, uses hot blast drying at last.
(4) put in the spectral investigator detecting base plate, carry out spectral detection.
5. according to claim 3, claim 4 described prostatic cancer early diagnosis kits, it is characterized in that fresh blood sample, fresh serum, the new blood of described sample solution for the detected person based on the ZnO nanometer stick array.
6. the prostatic cancer early diagnosis kit based on the ZnO nanometer stick array according to claim 2 is characterized in that, the included damping fluid of this kit is made up of following ingredients: Na
2HPO
40.194g; KH
2PO
40.0224g; Redistilled water 100mL regulates pH value to 7.3.
7. the prostatic cancer early diagnosis kit based on the ZnO nanometer stick array according to claim 1, it is characterized in that, the preparation of the modification ZnO nano-stick array thin film of prostate specific antibody (anti-PSA) is to adopt sol-gel process to generate the ZnO nano-stick array thin film on glass film plates, adopt the polypeptide condensation method that prostate specific antibody (anti-PSA) is connected on the ZnO nanorod surfaces by chemical bond-linking then, form the ZnO nano-stick array thin film of biomolecule modification.
8. the prostatic cancer early diagnosis kit based on the ZnO nanometer stick array according to claim 4, it is characterized in that, detection method also comprises step: (5) compare the spectral signal of mensuration and the spectral signal of on-gauge plate, thereby determine in the blood sample to be measured whether prostate specific antigen exists and/quantity.
9. the prostatic cancer early diagnosis kit based on the ZnO nanometer stick array according to claim 4 is characterized in that, described detectable signal is the photoluminescence signal of ZnO nanometer rods.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CNA2007100496596A CN101358974A (en) | 2007-08-02 | 2007-08-02 | Prostatic cancer early diagnosis kit based on ZnO nano-bar array |
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| CNA2007100496596A CN101358974A (en) | 2007-08-02 | 2007-08-02 | Prostatic cancer early diagnosis kit based on ZnO nano-bar array |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102866153A (en) * | 2012-09-29 | 2013-01-09 | 郑州安图绿科生物工程有限公司 | Prostatitis joint-detection kit |
| CN103616427A (en) * | 2013-12-02 | 2014-03-05 | 中国科学院上海应用物理研究所 | Micro-fluid control electrochemical biological sensing system for simultaneous detection on different serum markers of prostate cancer |
-
2007
- 2007-08-02 CN CNA2007100496596A patent/CN101358974A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102866153A (en) * | 2012-09-29 | 2013-01-09 | 郑州安图绿科生物工程有限公司 | Prostatitis joint-detection kit |
| CN103616427A (en) * | 2013-12-02 | 2014-03-05 | 中国科学院上海应用物理研究所 | Micro-fluid control electrochemical biological sensing system for simultaneous detection on different serum markers of prostate cancer |
| CN103616427B (en) * | 2013-12-02 | 2016-05-04 | 中国科学院上海应用物理研究所 | A kind of micro-fluidic electrochemica biological sensor-based system simultaneously detecting for the dissimilar blood serum designated object of prostate cancer |
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Application publication date: 20090204 |