CN101357192A - Separation preparation method of gastrodia elata crude polysaccharide and gastrodia elata alcohol extract using fresh gastrodia elata as raw material - Google Patents
Separation preparation method of gastrodia elata crude polysaccharide and gastrodia elata alcohol extract using fresh gastrodia elata as raw material Download PDFInfo
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- CN101357192A CN101357192A CNA2008100583211A CN200810058321A CN101357192A CN 101357192 A CN101357192 A CN 101357192A CN A2008100583211 A CNA2008100583211 A CN A2008100583211A CN 200810058321 A CN200810058321 A CN 200810058321A CN 101357192 A CN101357192 A CN 101357192A
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Abstract
The invention relates to a separation and preparation method of crude polysaccharide of gastrodia tuber which takes fresh gastrodia tuber as the raw material and a gastrodia tuber alcohol-soluble substance. The technical scheme of the invention is characterized in that the collected fresh gastrodia tuber is washed, deactivated to remove enzymes by a microwave method or a cooling process, crashed and pulped into pulp with water, and the pulp is roughly filtered by a three-column centrifuge and finely filtered by a tubular centrifuge to obtain a clear solution, which is added with 95% ethanol 3 times the volume of the clear solution while being stirred and kept standing to precipitate the crude polysaccharide of the gastrodia tuber; the crude polysaccharide is dried and crashed to obtain crude polysaccharide powder of the gastrodia tuber; diacolation extraction is carried out on the filter residue of the gastrodia tuber by the ethanol-water clear solution which is used for precipitating the polysaccharide so as to obtain an extracting solution, and the extracting solution is condensed, dried and crashed to obtain the gastrodia tuber alcohol-soluble powder. The effect and use of the crude polysaccharide of the gastrodia tuber can provide a base material for single and compound Chinese patent medicine products or healthcare foods which are made of the gastrodia tuber and have different treatment effects and different actions. The crude polysaccharide of the gastrodia tuber has the advantages of low amount requirement and easy absorption in the precondition that efficacies of the gastrodia tuber are guaranteed, thus ensuring effects of the gastrodia tuber and improving the physiological effects of the gastrodia tuber on human body.
Description
Technical field
The present invention relates to the Rhizoma Gastrodiae is the Rhizoma Gastrodiae crude polysaccharides of raw material and the method for separating and preparing of Rhizoma Gastrodiae alcohol soluble substance, belongs to the vegetable active composition and extracts manufacture field.
Technical background
Rhizoma Gastrodiae is as famous and precious Chinese medicine raw material, have sedation, anticonvulsant action, analgesic activity, antiinflammatory action, delaying cell aging effect and to the effect of higher nervous center and cardiovascular system, confirmed by the modern pharmacology institute, effect is remarkable, in modern medical service is clinical, obtained using more widely, as products such as gastrodia buccal tablet, gastrodia liquor, gastrodia royal jelly, gastrodia capsules.But, the raw material that these products utilized depends on traditional Rhizoma Gastrodiae raw material processing technique more, this technology comprise Rhizoma Gastrodiae gather after through dry 4-5 days of washing, steaming or the well-done heart, shaping, coal fire, what have also wants sulphuring, to guarantee the storage and the color and luster of raw material.
Along with the restriction of food and drug safety aspect to strict control use sulfide in the raw material processing procedure, traditional Rhizoma Gastrodiae processing technique is under suspicion.Especially the Rhizoma Gastrodiae crude polysaccharides that carries out based on different efficacies and the method for separating and preparing of Rhizoma Gastrodiae alcohol soluble substance, report not also at present, the method for separating and preparing that for this reason with the Rhizoma Gastrodiae is the Rhizoma Gastrodiae crude polysaccharides of raw material and Rhizoma Gastrodiae alcohol soluble substance are a kind of useful examinations of repaying.
Summary of the invention
But the objective of the invention is by the strong Technology of simple and feasible action row, the Rhizoma Gastrodiae crude polysaccharides and the two big active components separation preparations of Rhizoma Gastrodiae alcohol soluble substance of different physiologically actives in the Rhizoma Gastrodiae.Under the prerequisite that guarantees the Rhizoma Gastrodiae effect, have few, the easy absorption of consumption, convenient storage, reduce and pollute the advantage of (as sulfide etc.), guaranteed the performance of Rhizoma Gastrodiae function, improved the physiological function of Rhizoma Gastrodiae to human body.
The present invention is achieved in that
(1) Rhizoma Gastrodiae is cleaned the back and complete, be as the criterion with the saturating heart of Rhizoma Gastrodiae with microwave method or cooking process;
(2) will complete back Rhizoma Gastrodiae fragmentation, add the centrifugal filtration of water mill slurry, twice different modes, be tripod pendulum type batch centrifugal coarse filtration and tube centrifuge fine straining, clear liquid under agitation adds three times of 95% ethanol of volume ratio, quiescent setting Rhizoma Gastrodiae crude polysaccharides, filter and take out the Rhizoma Gastrodiae crude polysaccharides, in normal pressure or vacuum, dry under the temperature 50-70 ℃ of condition, the broken Rhizoma Gastrodiae crude polysaccharide powder that obtains;
(3) the Rhizoma Gastrodiae filtering residue carries out percolation with the pure water clear liquid of precipitation behind the polysaccharide and extracts, Rhizoma Gastrodiae alcohol soluble substance extracting parameter is: pure its concentration of alcohol of water clear liquid is 70%-75%, alcohol water clear liquid and Rhizoma Gastrodiae filtering residue weight ratio are 15-20,2-3 hour percolation time, percolation number of times 2 times, and extracting solution is through concentrated, dry down, the broken Rhizoma Gastrodiae alcohol soluble substance powder that obtains of vacuum condition.
The invention has the beneficial effects as follows: but the Technology by a kind of simple use, technical feasibility industrializing implementation is separated into two kinds of components with the Rhizoma Gastrodiae extracts active ingredients.Its advantage is 1, avoid the pollution of Rhizoma Gastrodiae sulphur compound in drying process.2, two of separation and Extraction kinds of components can for the different curative effects of Rhizoma Gastrodiae, not same-action health food or Chinese patent medicine is single, the prescription goods provide basic material, under the prerequisite that guarantees the Rhizoma Gastrodiae effect, have that consumption is few, the advantage of easy absorption, guaranteed the performance of Rhizoma Gastrodiae function, improved the physiological function of Rhizoma Gastrodiae human body.3, complete to the extracts active ingredients of Rhizoma Gastrodiae raw material, reduce the wasting of resources.
Specific embodiment
Embodiment one
Rhizoma Gastrodiae 60kg is cleaned the back complete, be as the criterion with the saturating heart of Rhizoma Gastrodiae with microwave method;
With the back Rhizoma Gastrodiae fragmentation that completes, add the centrifugal filtration of water mill slurry, twice different modes, be tripod pendulum type batch centrifugal coarse filtration and tube centrifuge fine straining, clear liquid under agitation adds three times of 95% ethanol of volume ratio, quiescent setting Rhizoma Gastrodiae crude polysaccharides, filter and take out the Rhizoma Gastrodiae crude polysaccharides, dry under normal pressure, 50 ℃ of conditions of temperature, the broken Rhizoma Gastrodiae crude polysaccharide powder that obtains;
The Rhizoma Gastrodiae filtering residue carries out percolation with the pure water clear liquid of precipitation behind the polysaccharide and extracts, Rhizoma Gastrodiae alcohol soluble substance extracting parameter is: pure its concentration of alcohol of water clear liquid is 70%, alcohol water clear liquid and Rhizoma Gastrodiae filtering residue weight ratio are 15,2 hours percolation time, percolation number of times 2 times, extracting solution through concentrating, dry down, the broken acquisition Rhizoma Gastrodiae of vacuum condition alcohol soluble substance powder.
Embodiment two
Rhizoma Gastrodiae is cleaned the back complete, be as the criterion with the saturating heart of Rhizoma Gastrodiae with cooking process;
With the back Rhizoma Gastrodiae fragmentation that completes, add the centrifugal filtration of water mill slurry, twice different modes, be tripod pendulum type batch centrifugal coarse filtration and tube centrifuge fine straining, clear liquid under agitation adds three times of 95% ethanol of volume ratio, quiescent setting Rhizoma Gastrodiae crude polysaccharides, filter and take out the Rhizoma Gastrodiae crude polysaccharides, dry under vacuum, 65 ℃ of conditions of temperature, the broken Rhizoma Gastrodiae crude polysaccharide powder that obtains;
The Rhizoma Gastrodiae filtering residue carries out percolation with the pure water clear liquid of precipitation behind the polysaccharide and extracts, Rhizoma Gastrodiae alcohol soluble substance extracting parameter is: pure its concentration of alcohol of water clear liquid is 75%, alcohol water clear liquid and Rhizoma Gastrodiae filtering residue weight ratio 20,3 hours percolation time, percolation number of times 2 times, extracting solution is through concentrated, dry down, the broken Rhizoma Gastrodiae alcohol soluble substance powder that obtains of vacuum condition.
Embodiment three
Rhizoma Gastrodiae is cleaned the back complete, be as the criterion with the saturating heart of Rhizoma Gastrodiae with microwave method;
With the back Rhizoma Gastrodiae fragmentation that completes, add the centrifugal filtration of water mill slurry, twice different modes, be tripod pendulum type batch centrifugal coarse filtration and tube centrifuge fine straining, clear liquid under agitation adds three times of 95% ethanol of volume ratio, quiescent setting Rhizoma Gastrodiae crude polysaccharides, filter and take out the Rhizoma Gastrodiae crude polysaccharides, dry under vacuum, 70 ℃ of conditions of temperature, the broken Rhizoma Gastrodiae crude polysaccharide powder that obtains;
The Rhizoma Gastrodiae filtering residue carries out percolation with the pure water clear liquid of precipitation behind the polysaccharide and extracts, Rhizoma Gastrodiae alcohol soluble substance extracting parameter is: pure its concentration of alcohol of water clear liquid is 72%, alcohol water clear liquid and Rhizoma Gastrodiae filtering residue weight ratio 18,2.6 hours percolation time, percolation number of times 2 times, extracting solution is through concentrated, dry down, the broken Rhizoma Gastrodiae alcohol soluble substance powder that obtains of vacuum condition.
Embodiment four
Rhizoma Gastrodiae is cleaned the back complete, be as the criterion with the saturating heart of Rhizoma Gastrodiae with cooking process;
With the back Rhizoma Gastrodiae fragmentation that completes, add the centrifugal filtration of water mill slurry, twice different modes, be tripod pendulum type batch centrifugal coarse filtration and tube centrifuge fine straining, clear liquid under agitation adds three times of 95% ethanol of volume ratio, quiescent setting Rhizoma Gastrodiae crude polysaccharides, filter and take out the Rhizoma Gastrodiae crude polysaccharides, dry under normal pressure, 65 ℃ of conditions of temperature, the broken Rhizoma Gastrodiae crude polysaccharide powder that obtains;
The Rhizoma Gastrodiae filtering residue carries out percolation with the pure water clear liquid of precipitation behind the polysaccharide and extracts, Rhizoma Gastrodiae alcohol soluble substance extracting parameter is: pure its concentration of alcohol of water clear liquid is 74%, alcohol water clear liquid and Rhizoma Gastrodiae filtering residue weight ratio 16,2.8 hours percolation time, percolation number of times 2 times, extracting solution is through concentrated, dry down, the broken Rhizoma Gastrodiae alcohol soluble substance powder that obtains of vacuum condition.
Claims (1)
1, a kind of is the Rhizoma Gastrodiae crude polysaccharides of raw material and the method for separating and preparing of Rhizoma Gastrodiae alcohol soluble substance with the Rhizoma Gastrodiae, and its main technique is:
(1) Rhizoma Gastrodiae is cleaned the back and complete, be as the criterion with the saturating heart of Rhizoma Gastrodiae with microwave method or cooking process;
(2) will complete back Rhizoma Gastrodiae fragmentation, add the centrifugal filtration of water mill slurry, twice different modes, be tripod pendulum type batch centrifugal coarse filtration and tube centrifuge fine straining, clear liquid under agitation adds three times of 95% ethanol of volume ratio, quiescent setting Rhizoma Gastrodiae crude polysaccharides, filter and take out the Rhizoma Gastrodiae crude polysaccharides, in normal pressure or vacuum, dry under the temperature 50-70 ℃ of condition, the broken Rhizoma Gastrodiae crude polysaccharide powder that obtains;
(3) the Rhizoma Gastrodiae filtering residue carries out percolation with the pure water clear liquid of precipitation behind the polysaccharide and extracts, Rhizoma Gastrodiae alcohol soluble substance extracting parameter is: pure its concentration of alcohol of water clear liquid is 70%-75%, alcohol water clear liquid and Rhizoma Gastrodiae filtering residue weight ratio are 15-20,2-3 hour percolation time, percolation number of times 2 times, and extracting solution is through concentrated, dry down, the broken Rhizoma Gastrodiae alcohol soluble substance powder that obtains of vacuum condition.
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| CN2008100583211A CN101357192B (en) | 2008-04-23 | 2008-04-23 | Separation preparation method of gastrodia elata crude polysaccharide and gastrodia elata alcohol extract using fresh gastrodia elata as raw material |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102464725A (en) * | 2010-11-17 | 2012-05-23 | 中国科学院上海药物研究所 | Gastrodia elata BL. polysaccharide and degradation products thereof and preparation methods and applications of degradation products |
| CN103242461A (en) * | 2012-02-06 | 2013-08-14 | 陕西理工学院 | Novel method for simultaneously extracting gastrodia elata polysaccharide and gastrodine |
| CN103535733A (en) * | 2013-11-01 | 2014-01-29 | 王颖 | Method for extracting essence material of gastrodia elata |
| CN103613679A (en) * | 2013-11-19 | 2014-03-05 | 刘祥义 | Gastrodia elata polysaccharide preparation method taking fresh gastrodia elata as raw material |
| CN105779541A (en) * | 2016-04-07 | 2016-07-20 | 金寨金栗源生物技术有限公司 | Gastrodia elata peptide preparation method |
| CN106243237A (en) * | 2016-08-03 | 2016-12-21 | 安徽徽王农业有限公司 | The manufacture method of blue berry polysaccharide |
| CN110013032A (en) * | 2018-12-19 | 2019-07-16 | 遵义茶溶天下生物科技有限公司 | A kind of preparation method for the Rhizoma Gastrodiae instant powder that boiling water homogenate is fixed |
-
2008
- 2008-04-23 CN CN2008100583211A patent/CN101357192B/en active Active
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102464725A (en) * | 2010-11-17 | 2012-05-23 | 中国科学院上海药物研究所 | Gastrodia elata BL. polysaccharide and degradation products thereof and preparation methods and applications of degradation products |
| CN102464725B (en) * | 2010-11-17 | 2013-10-30 | 中国科学院上海药物研究所 | Gastrodia elata BL. polysaccharide and degradation products thereof and preparation methods and applications of degradation products |
| CN103242461A (en) * | 2012-02-06 | 2013-08-14 | 陕西理工学院 | Novel method for simultaneously extracting gastrodia elata polysaccharide and gastrodine |
| CN103535733A (en) * | 2013-11-01 | 2014-01-29 | 王颖 | Method for extracting essence material of gastrodia elata |
| CN103613679A (en) * | 2013-11-19 | 2014-03-05 | 刘祥义 | Gastrodia elata polysaccharide preparation method taking fresh gastrodia elata as raw material |
| CN103613679B (en) * | 2013-11-19 | 2016-03-16 | 刘祥义 | A kind of gastrodia elata polysaccharide preparation method that is raw material with fresh rhizoma Gastrodiae |
| CN105779541A (en) * | 2016-04-07 | 2016-07-20 | 金寨金栗源生物技术有限公司 | Gastrodia elata peptide preparation method |
| CN106243237A (en) * | 2016-08-03 | 2016-12-21 | 安徽徽王农业有限公司 | The manufacture method of blue berry polysaccharide |
| CN110013032A (en) * | 2018-12-19 | 2019-07-16 | 遵义茶溶天下生物科技有限公司 | A kind of preparation method for the Rhizoma Gastrodiae instant powder that boiling water homogenate is fixed |
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Effective date of registration: 20160726 Address after: 650223 Kunming municipal road, Yunnan, No. 14 Patentee after: Scientific Research Institute of Supply and Marketing Cooperative Society of Yunnan Province Patentee after: Kunming Edible Fungus Institute of China Federation of Supply and Marketing Cooperatives Address before: 650223 Kunming municipal road, Yunnan, No. 14 Patentee before: Kunming Fungus Food Co., Ltd. Patentee before: Scientific Research Institute of Supply and Marketing Cooperative Society of Yunnan Province Patentee before: Kunming Edible Fungus Institute of China Federation of Supply and Marketing Cooperatives |