CN101357181B - Medicine containing bupleurum root for treating diabetes and preparation method thereof - Google Patents
Medicine containing bupleurum root for treating diabetes and preparation method thereof Download PDFInfo
- Publication number
- CN101357181B CN101357181B CN2007100585725A CN200710058572A CN101357181B CN 101357181 B CN101357181 B CN 101357181B CN 2007100585725 A CN2007100585725 A CN 2007100585725A CN 200710058572 A CN200710058572 A CN 200710058572A CN 101357181 B CN101357181 B CN 101357181B
- Authority
- CN
- China
- Prior art keywords
- radix
- portions
- rhizoma
- root
- fructus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000003814 drug Substances 0.000 title claims abstract description 29
- 206010012601 diabetes mellitus Diseases 0.000 title claims abstract description 17
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 229940079593 drug Drugs 0.000 title abstract description 8
- 241000202726 Bupleurum Species 0.000 title abstract 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 49
- 238000010992 reflux Methods 0.000 claims abstract description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000000463 material Substances 0.000 claims abstract description 6
- 239000000706 filtrate Substances 0.000 claims abstract description 5
- 239000010135 fructus aurantii immaturus Substances 0.000 claims description 26
- VYQNWZOUAUKGHI-UHFFFAOYSA-N monobenzone Chemical compound C1=CC(O)=CC=C1OCC1=CC=CC=C1 VYQNWZOUAUKGHI-UHFFFAOYSA-N 0.000 claims description 26
- 239000008517 radix Trichosanthis Substances 0.000 claims description 26
- 239000000284 extract Substances 0.000 claims description 17
- 239000012567 medical material Substances 0.000 claims description 13
- 235000008216 herbs Nutrition 0.000 claims description 5
- 241001522129 Pinellia Species 0.000 abstract description 4
- 238000000605 extraction Methods 0.000 abstract description 4
- 239000000203 mixture Substances 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 3
- 235000013399 edible fruits Nutrition 0.000 abstract 3
- 244000183685 Citrus aurantium Species 0.000 abstract 2
- 235000007716 Citrus aurantium Nutrition 0.000 abstract 2
- 241000037740 Coptis chinensis Species 0.000 abstract 2
- 244000236658 Paeonia lactiflora Species 0.000 abstract 2
- 235000008598 Paeonia lactiflora Nutrition 0.000 abstract 2
- 235000008081 Rheum officinale Nutrition 0.000 abstract 2
- 240000001745 Rheum palmatum Species 0.000 abstract 2
- 240000004534 Scutellaria baicalensis Species 0.000 abstract 2
- 235000017089 Scutellaria baicalensis Nutrition 0.000 abstract 2
- 235000009917 Crataegus X brevipes Nutrition 0.000 abstract 1
- 235000013204 Crataegus X haemacarpa Nutrition 0.000 abstract 1
- 235000009685 Crataegus X maligna Nutrition 0.000 abstract 1
- 235000009444 Crataegus X rubrocarnea Nutrition 0.000 abstract 1
- 235000009486 Crataegus bullatus Nutrition 0.000 abstract 1
- 235000017181 Crataegus chrysocarpa Nutrition 0.000 abstract 1
- 235000009682 Crataegus limnophila Nutrition 0.000 abstract 1
- 235000004423 Crataegus monogyna Nutrition 0.000 abstract 1
- 240000000171 Crataegus monogyna Species 0.000 abstract 1
- 235000002313 Crataegus paludosa Nutrition 0.000 abstract 1
- 235000009840 Crataegus x incaedua Nutrition 0.000 abstract 1
- 241000218989 Trichosanthes Species 0.000 abstract 1
- 230000006837 decompression Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 34
- 239000008280 blood Substances 0.000 description 10
- 210000004369 blood Anatomy 0.000 description 10
- 238000001914 filtration Methods 0.000 description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 7
- 229960004756 ethanol Drugs 0.000 description 7
- 239000008103 glucose Substances 0.000 description 7
- 235000009604 Chrysanthemum X morifolium Nutrition 0.000 description 4
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- ZSJLQEPLLKMAKR-GKHCUFPYSA-N streptozocin Chemical compound O=NN(C)C(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O ZSJLQEPLLKMAKR-GKHCUFPYSA-N 0.000 description 4
- 241000207929 Scutellaria Species 0.000 description 3
- 102000011759 adducin Human genes 0.000 description 3
- 108010076723 adducin Proteins 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- -1 Radix Bupleuri Substances 0.000 description 2
- ZSJLQEPLLKMAKR-UHFFFAOYSA-N Streptozotocin Natural products O=NN(C)C(=O)NC1C(O)OC(CO)C(O)C1O ZSJLQEPLLKMAKR-UHFFFAOYSA-N 0.000 description 2
- 206010067584 Type 1 diabetes mellitus Diseases 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 229960000935 dehydrated alcohol Drugs 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- XZWYZXLIPXDOLR-UHFFFAOYSA-N metformin Chemical compound CN(C)C(=N)NC(N)=N XZWYZXLIPXDOLR-UHFFFAOYSA-N 0.000 description 2
- 229960003105 metformin Drugs 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 229960001052 streptozocin Drugs 0.000 description 2
- 208000035408 type 1 diabetes mellitus 1 Diseases 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 229940123208 Biguanide Drugs 0.000 description 1
- 208000002705 Glucose Intolerance Diseases 0.000 description 1
- 102000004366 Glucosidases Human genes 0.000 description 1
- 108010056771 Glucosidases Proteins 0.000 description 1
- 206010018473 Glycosuria Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000594182 Sarcophaga sigma Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 238000002266 amputation Methods 0.000 description 1
- 229960004543 anhydrous citric acid Drugs 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000004283 biguanides Chemical class 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000001610 euglycemic effect Effects 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 230000003345 hyperglycaemic effect Effects 0.000 description 1
- 201000001421 hyperglycemia Diseases 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- VIKNJXKGJWUCNN-XGXHKTLJSA-N norethisterone Chemical compound O=C1CC[C@@H]2[C@H]3CC[C@](C)([C@](CC4)(O)C#C)[C@@H]4[C@@H]3CCC2=C1 VIKNJXKGJWUCNN-XGXHKTLJSA-N 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 description 1
- 201000009104 prediabetes syndrome Diseases 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 238000012109 statistical procedure Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229940124530 sulfonamide Drugs 0.000 description 1
- 150000003456 sulfonamides Chemical class 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a medicine containing bupleurum root and used for treating diabetes and a preparation method thereof. The medicine is made by the following method: (1) medicinal materials are selected, and the medicinal materials comprise the following crude drugs by weight portions: 5-40 portions of trichosanthes root, 10-30 portions of bupleurum root, 3-15 portions of fruit of immature bitter orange, 1-6 portions of Chinese rhubarb, 1-12 portions of pinellia tuber, 3-15 portions of baikal skullcap root, 1-12 portions of Chinese goldthread, 3-15 portions of white peony root and 5-20 portions of dark plum; (2) refluxing extraction is carried out on the Bupleurum root, the white peony root, the fruit of immature bitter orange, the Chinese rhubarb, the baikal skullcap root and Chinese goldthread by ethanol with a certain concentration to obtain extracting solutions, and then the obtained extracting solutions are cooled, filtered and mixed to obtain a filtrate; (3) dregs obtained from the refluxing extraction are added with other medicinal materials and water for refluxing extraction to obtain an extracting solution, the extracting solution is condensed by decompression to obtain a concentrated solution, the concentrated solution is added with the ethanol until the ethanol content reaches 65-75% to obtain a mixture, and the mixture is filtered to obtain a filtrate; (4) the filtrates obtained from (2) and (3) are mixed and condensed into an extractum. The medicinal materials of the medicine can be further added with hawthorn fruit.
Description
Technical field
The present invention relates to a kind of medicine, be specifically related to a kind of medicine for the treatment of diabetes and preparation method thereof.
Background technology
Diabetes are the lifelong diseases of a kind of chronic progressive external that caused by sugar, protein and fat metabolic disturbance in the body, and its characteristic shows as hyperglycemia and glycosuria.This disease will cause heart and renal complication, blind and amputation.From observing clinically, the sickness rate of diabetes is being ascendant trend year by year in recent years.At present, the whole world has 1.5 hundred million diabeticss approximately, and the present diabetes number of China surpasses 4,000 ten thousand.In diabetes cases, minority is insulin dependent diabetes mellitus (IDDM) (an I type), and great majority are non-insulin-dependent diabetes mellitus (II type).Most diabetics need be taken medicine all the life.
Treating diabetes is still based on chemical synthetic drug, as sulfonamides, biguanides, glucosidase inhibitor, Lie Nai and euglycemic agent etc. at present.But it is all bigger that these medicines are taken side effect for a long time.
Chinese medicine has long history aspect the diabetes preventing and treating, and the practice through several thousand has accumulated a large amount of invaluable experiences, has formed the unique academic system.The Chinese medicine ancient books and records have been put down in writing the Chinese medicine that much has the blood sugar lowering curative effect, as Rhizoma Alismatis, the Rhizoma Anemarrhenae, Radix Panacis Quinquefolii, Radix Puerariae, Radix Rehmanniae, pollen etc.Traditional TCM treatment of diabetes is to carry out the three types of diabetes opinion according to clinical symptoms to control.But there is not good treatment way in early days at impaired glucose tolerance phase and onset diabetes.
The clearing stomach of Therapeutic Method dissipating depression of QI, nourishing YIN to lower pathogenic fire, purging FU-organs rushes down turbid.
The inventor is a theoretical foundation with the traditional Chinese medical science " hot and suffocating " theory, discovers that the compositions of being made up of medical materials such as Radix Trichosanthis, Radix Bupleuri, Fructus Aurantii Immaturus, Radix Et Rhizoma Rhei, the Rhizoma Pinelliae, Radix Scutellariae, Rhizoma Coptidis, the Radix Paeoniae Alba, Fructus Mumes has good therapeutical effect to diabetes.But the extract by traditional pure extracting method obtains has lost a large amount of aqueous soluble active constituents; Not only yield is low, and content of effective is very little, so dosage is very big, corresponding side effect also can increase.
Summary of the invention
The object of the present invention is to provide a kind of medicine for the treatment of diabetes, adopt following method to be prepared from:
(1) material of getting it filled, described medical material contains the raw medicinal herbs of following weight portion: Radix Trichosanthis 5-40 part, Radix Bupleuri 10-30 part, Fructus Aurantii Immaturus 3-15 part, Radix Et Rhizoma Rhei 1-6 part, Rhizoma Pinelliae 1-12 part, Radix Scutellariae 3-15 part, Rhizoma Coptidis 1-12 part, Radix Paeoniae Alba 3-15 part, Fructus Mume 5-20 part;
(2) wherein Radix Bupleuri, the Radix Paeoniae Alba, Fructus Aurantii Immaturus, Radix Et Rhizoma Rhei, Radix Scutellariae, Rhizoma Coptidis are used certain density alcohol reflux, extracting solution cools, filters, merges;
(3) add other medical materials in the medicinal residues, add the water reflux, extract,, the extracting solution concentrating under reduced pressure, it is 65-75% to containing pure weight that concentrated solution adds ethanol, filters;
(4) (2) and (3) gained filtrate is merged, be condensed into extractum.
In the step (2), concentration of alcohol is preferably 75-90%, and more preferably 80%; Preferred reflux, extract, twice, each 10 times of medical material weight were extracted 1.5 hours at every turn.
In the step (3), preferred reflux, extract, twice, each 10 times of medical material weight were extracted 1.5 hours at every turn.The ratio that preferred extracting solution is concentrated into the volume of initial medical material weight and concentrated solution is 1:1.The concentration of alcohol that adds is preferably 90-100%, and more preferably 95%.Contain pure weight and be preferably 70%.
Described medicine, the weight ratio of each raw medicinal herbs is preferably: 9 parts of Radix Trichosanthis, 12 parts of Radix Bupleuri, 9 parts of Fructus Aurantii Immaturuss, 3 parts of Radix Et Rhizoma Rhei, 6 parts of the Rhizoma Pinelliaes, 9 parts of Radix Scutellariaes, 6 parts of Rhizoma Coptidis, 9 parts of the Radix Paeoniae Albas, 9 parts of Fructus Mumes.
Better, the present invention can also add Fructus Crataegi on the basis of described medicine, and this is because the Fructus Crataegi sour and sweet drugs can transforme into YIN, both can be in case the hot too impairment of YIN of loosing, but picric acid system is sweet again.The heat clearing away of compatibility dissipating depression of QI has better curative effect.The weight portion of each raw medicinal herbs is: Radix Trichosanthis 5-40 part, Radix Bupleuri 10-30 part, Fructus Aurantii Immaturus 3-15 part, Radix Et Rhizoma Rhei 1-6 part, Rhizoma Pinelliae 1-12 part, Radix Scutellariae 3-15 part, Rhizoma Coptidis 1-12 part, Radix Paeoniae Alba 3-15 part, Fructus Mume 5-20 part, Fructus Crataegi 3-15 part.
Be preferably: Radix Trichosanthis 10-30 part, Radix Bupleuri 10-30 part, Fructus Aurantii Immaturus 3-15 part, Radix Et Rhizoma Rhei 1-6 part, Rhizoma Pinelliae 1-12 part, Radix Scutellariae 3-15 part, Rhizoma Coptidis 1-12 part, Radix Paeoniae Alba 3-15 part, Fructus Mume 5-20 part, Fructus Crataegi 3-15 part.
More preferably: 30 parts of Radix Trichosanthis, 12 parts of Radix Bupleuri, 9 parts of Fructus Aurantii Immaturuss, 3 parts of Radix Et Rhizoma Rhei, 6 parts of the Rhizoma Pinelliaes, 9 parts of Radix Scutellariaes, 6 parts of Rhizoma Coptidis, 9 parts of the Radix Paeoniae Albas, 15 parts of Fructus Mumes, 9 parts of Fructus Crataegis; Perhaps 15 parts of Radix Trichosanthis, 12 parts of Radix Bupleuri, 9 parts of Fructus Aurantii Immaturuss, 3 parts of Radix Et Rhizoma Rhei, 6 parts of the Rhizoma Pinelliaes, 9 parts of Radix Scutellariaes, 6 parts of Rhizoma Coptidis, 9 parts of the Radix Paeoniae Albas, 15 parts of Fructus Mumes, 9 parts of Fructus Crataegis.
Preparation method of the present invention, the alcohol extraction of raw medicinal herbs such as Radix Bupleuri, the Radix Paeoniae Alba, Fructus Aurantii Immaturus, Radix Et Rhizoma Rhei, Radix Scutellariae, Rhizoma Coptidis elder generation, residue merges water with other medical materials again to be carried, and has guaranteed that this Six-element medical material effective ingredient is fully extracted; The effective ingredient of other medical materials during only water is carried, this is because effective ingredient wherein all is a water solublity.Content of effective obviously improves in the Chinese medicine composition of the present invention, and the content of impurity significantly reduces, thereby has reduced the dosage of administration.
Medicine of the present invention can adopt the conventional method of Chinese medicine preparation to be prepared into the preparation of any routine.For example the above-mentioned raw materials medicine is ground into powder and takes after mixing it with water, or make tablet or capsule oral, perhaps make injection etc.But these can not be used to limit protection scope of the present invention.
The specific embodiment
Further specify medicine of the present invention and beneficial effect thereof below by embodiment and test example, but not as limitation of the present invention.
Embodiment 1
Radix Trichosanthis 750g, Radix Bupleuri 1500g, Fructus Aurantii Immaturus 450g, Radix Et Rhizoma Rhei 150g, Rhizoma Pinelliae 150g, Radix Scutellariae 450g, Rhizoma Coptidis 150g, Radix Paeoniae Alba 450g, Fructus Mume 750g
Radix Bupleuri, the Radix Paeoniae Alba, Fructus Aurantii Immaturus, Radix Et Rhizoma Rhei, Radix Scutellariae, Rhizoma Coptidis were measured 75% alcohol reflux 2 times each 1.5 hours with 10 times, and extracting solution filters, and is standby; Medicinal residues add Radix Trichosanthis, Fructus Mume, the Rhizoma Pinelliae, and with 10 times of water gaging reflux, extract, 2 times each 1.5 hours, extracting solution was evaporated to 1:1, add 90% ethanol to containing alcohol amount 65%, and filtration is standby; Two extracting solution are mixed, and concentrating under reduced pressure becomes thick paste.
Embodiment 2
Radix Trichosanthis 1200g, Radix Bupleuri 900g, Fructus Aurantii Immaturus 450g, Radix Et Rhizoma Rhei 180g, Rhizoma Pinelliae 360g, Radix Scutellariae 450g, Rhizoma Coptidis 360g, Radix Paeoniae Alba 450g, Fructus Mume 600g
Radix Bupleuri, the Radix Paeoniae Alba, Fructus Aurantii Immaturus, Radix Et Rhizoma Rhei, Radix Scutellariae, Rhizoma Coptidis were measured 90% alcohol reflux 2 times each 1.5 hours with 10 times, and extracting solution filters, and is standby; Medicinal residues add Radix Trichosanthis, Fructus Mume, the Rhizoma Pinelliae, and with 10 times of water gaging reflux, extract, 2 times each 1.5 hours, extracting solution was evaporated to 1:1, add dehydrated alcohol to containing alcohol amount 75%, and filtration is standby; Two extracting solution are mixed, and concentrating under reduced pressure becomes thick paste.
Embodiment 3
Radix Trichosanthis 630g Radix Bupleuri 840g Fructus Aurantii Immaturus 630g Radix Et Rhizoma Rhei 210g Rhizoma Pinelliae 420g Radix Scutellariae 630g Rhizoma Coptidis 420g Radix Paeoniae Alba 630g Fructus Mume 630g
Radix Bupleuri, the Radix Paeoniae Alba, Fructus Aurantii Immaturus, Radix Et Rhizoma Rhei, Radix Scutellariae, Rhizoma Coptidis were measured 80% alcohol reflux 2 times each 1.5 hours with 10 times, and extracting solution filters, and is standby; Medicinal residues add Radix Trichosanthis, Fructus Mume, the Rhizoma Pinelliae, and with 10 times of water gaging reflux, extract, 2 times each 1.5 hours, extracting solution was evaporated to 1:1, add 95% ethanol to containing alcohol amount 70%, and filtration is standby; Two extracting solution are mixed, and concentrating under reduced pressure becomes thick paste.
Embodiment 4
Radix Trichosanthis 750g, Radix Bupleuri 1500g, Fructus Aurantii Immaturus 450g, Radix Et Rhizoma Rhei 150g, Rhizoma Pinelliae 150g, Radix Scutellariae 450g, Rhizoma Coptidis 150g, Radix Paeoniae Alba 450g, Fructus Mume 750g, Fructus Crataegi 450g
Radix Bupleuri, the Radix Paeoniae Alba, Fructus Aurantii Immaturus, Radix Et Rhizoma Rhei, Radix Scutellariae, Rhizoma Coptidis were measured 75% alcohol reflux 2 times each 1.5 hours with 10 times, and extracting solution filters, and is standby; Medicinal residues add Radix Trichosanthis, Fructus Mume, the Rhizoma Pinelliae, Fructus Crataegi, and with 10 times of water gaging reflux, extract, 2 times each 1.5 hours, extracting solution was evaporated to 1:1, add 90% alcohol to containing alcohol amount 65%, and filtration is standby; Two extracting solution are mixed, and concentrating under reduced pressure becomes thick paste.
Embodiment 5
Radix Trichosanthis 1200g, Radix Bupleuri 900g, Fructus Aurantii Immaturus 450g, Radix Et Rhizoma Rhei 180g, Rhizoma Pinelliae 360g, Radix Scutellariae 450g, Rhizoma Coptidis 360g, Radix Paeoniae Alba 450g, Fructus Mume 600g, Fructus Crataegi 450g
Radix Bupleuri, the Radix Paeoniae Alba, Fructus Aurantii Immaturus, Radix Et Rhizoma Rhei, Radix Scutellariae, Rhizoma Coptidis were measured 90% alcohol reflux 2 times each 1.5 hours with 10 times, and extracting solution filters, and is standby; Medicinal residues add Radix Trichosanthis, Fructus Mume, the Rhizoma Pinelliae, Fructus Crataegi, and with 10 times of water gaging reflux, extract, 2 times each 1.5 hours, extracting solution was evaporated to 1:1, add dehydrated alcohol to containing alcohol amount 75%, and filtration is standby; Two extracting solution are mixed, and concentrating under reduced pressure becomes thick paste.
Embodiment 6
Radix Trichosanthis 833g Radix Bupleuri 667g Fructus Aurantii Immaturus 500g Radix Et Rhizoma Rhei 167g Rhizoma Pinelliae 333g Radix Scutellariae 500g Rhizoma Coptidis 333g Radix Paeoniae Alba 500g Fructus Mume 833g Fructus Crataegi 500g
Radix Bupleuri, the Radix Paeoniae Alba, Fructus Aurantii Immaturus, Radix Et Rhizoma Rhei, Radix Scutellariae, Rhizoma Coptidis were measured 80% alcohol reflux 2 times each 1.5 hours with 10 times, and extracting solution filters, and is standby; Medicinal residues add Radix Trichosanthis, Fructus Mume, the Rhizoma Pinelliae, Fructus Crataegi, and with 10 times of water gaging reflux, extract, 2 times each 1.5 hours, extracting solution was evaporated to 1:1, add 95% ethanol to containing alcohol amount 70%, and filtration is standby; Two extracting solution are mixed, and concentrating under reduced pressure becomes thick paste.
Embodiment 7
Radix Trichosanthis 500g, Radix Bupleuri 750g, Fructus Aurantii Immaturus 500g, Radix Et Rhizoma Rhei 250g, Rhizoma Pinelliae 500g part, Radix Scutellariae 500g, Rhizoma Coptidis 500g, Radix Paeoniae Alba 500g, Fructus Mume 500g, Fructus Crataegi 500g
Radix Bupleuri, the Radix Paeoniae Alba, Fructus Aurantii Immaturus, Radix Et Rhizoma Rhei, Radix Scutellariae, Rhizoma Coptidis were measured 80% alcohol reflux 2 times each 1.5 hours with 10 times, and extracting solution filters, and is standby; Medicinal residues add Radix Trichosanthis, Fructus Mume, the Rhizoma Pinelliae, Fructus Crataegi, and with 10 times of water gaging reflux, extract, 2 times each 1.5 hours, extracting solution was evaporated to 1:1, add 95% ethanol to containing alcohol amount 70%, and filtration is standby; Two extracting solution are mixed, and concentrating under reduced pressure becomes thick paste.
Embodiment 8
Radix Trichosanthis 1200g, Radix Bupleuri 800g, Fructus Aurantii Immaturus 600g, Radix Et Rhizoma Rhei 200g, Rhizoma Pinelliae 400g, Radix Scutellariae 400g, Rhizoma Coptidis 200g, Radix Paeoniae Alba 400g, Fructus Mume 400g, Fructus Crataegi 400g
Radix Bupleuri, the Radix Paeoniae Alba, Fructus Aurantii Immaturus, Radix Et Rhizoma Rhei, Radix Scutellariae, Rhizoma Coptidis were measured 85% alcohol reflux 2 times each 1.5 hours with 10 times, and extracting solution filters, and is standby; Medicinal residues add Radix Trichosanthis, Fructus Mume, the Rhizoma Pinelliae, Fructus Crataegi, and with 10 times of water gaging reflux, extract, 2 times each 1.5 hours, extracting solution was evaporated to 1:1, add 90% ethanol to containing alcohol amount 68%, and filtration is standby; Two extracting solution are mixed, and concentrating under reduced pressure becomes thick paste.
Embodiment 9
Radix Trichosanthis 1000g, Radix Bupleuri 600g, Fructus Aurantii Immaturus 600g, Radix Et Rhizoma Rhei 250g, Rhizoma Pinelliae 300g, Radix Scutellariae 500g, Rhizoma Coptidis 300g, Radix Paeoniae Alba 500g, Fructus Mume 500g, Fructus Crataegi 300g
Radix Bupleuri, the Radix Paeoniae Alba, Fructus Aurantii Immaturus, Radix Et Rhizoma Rhei, Radix Scutellariae, Rhizoma Coptidis were measured 90% alcohol reflux 2 times each 1.5 hours with 10 times, and extracting solution filters, and is standby; Medicinal residues add Radix Trichosanthis, Fructus Mume, the Rhizoma Pinelliae, Fructus Crataegi, and with 10 times of water gaging reflux, extract, 2 times each 1.5 hours, extracting solution was evaporated to 1:1, add 99% ethanol to containing alcohol amount 74%, and filtration is standby; Two extracting solution are mixed, and concentrating under reduced pressure becomes thick paste.
Test example 1
1) laboratory animal:
SPF level male SD rat, body weight 200-220g.
2) main agents and medicine:
Streptozotocin (Streptozotocin, STZ): U.S. sigma company, lot number 024K1211;
Steady milli type blood glucose meter (One-Touch Ultra): U.S. Lifescan company, number of registration 20032400735;
Anhydrous citric acid (C
6H
8O
7.H
2O): Tianjin chemical reagent one factory, lot number 011121;
Sodium citrate (Na
3C
6H
5O
7.2H
2O): Tianjin chemical reagent one factory, lot number 011219;
Metformin: the 250mg/ sheet, Tianjin Pacific Pharmaceutical Co., Ltd. produces, lot number 040121;
The difference prescription group medicine of test example is respectively the extract powder according to embodiment 3, embodiment 8 and embodiment 9 preparations, and numbering is respectively TL-1, TL-2 and TL-3.
3) model preparation:
The SD rat adapts to the back fasting overnight of 1 week, and through lumbar injection STZ65mg/Kg (0.1mmol/l, PH4.4 citrate buffer solution are made the solution of 2% (W/V)), the tail vein is got blood to survey blood glucose 〉=16.7mmol/L be the modeling success after 3 days.
4) medication and observation index:
The diabetes rat of Cheng Mo is divided into model group, metformin 125mg/Kg group and different prescription groups (crude drug 15.5g/Kg) at random.Adapt to a week, detect gastric infusion behind the blood glucose, once a day, medicine all is mixed with the suspension administration with normal saline, the experimental session drinking-water of all freely ingesting, 15 days experimental periods.Administration the 7th, 14 days, fasting was 2 hours after the administration, and the tail vein is got blood one-touchultra blood glucose meter and measured blood glucose, detected the body weight of rat simultaneously.
5) statistical procedures:
(X ± SD) expression, two group difference significances adopt the t check to experimental data with mean ± standard deviation.
6) result
By table 1 as seen, different prescription groups are when 15.5g/Kg dosage, and administration all made the blood glucose of STZ hyperglycemic rat obviously descend (P＜0.01) after 7,14 days.
Table 1. is respectively organized changes of blood glucose and comparison (X ± SDn=10)
Annotate: compare with matched group
*P＜0.01; Compare with model group
▲P＜0.01
Claims (4)
1. a preparation method that is used for the treatment of the medicine of diabetes is characterized in that, may further comprise the steps:
(1) material of getting it filled, described medical material contains the raw medicinal herbs of following weight portion: Radix Trichosanthis 5-40 part, Radix Bupleuri 10-30 part, Fructus Aurantii Immaturus 3-15 part, Radix Et Rhizoma Rhei 1-6 part, Rhizoma Pinelliae 1-12 part, Radix Scutellariae 3-15 part, Rhizoma Coptidis 1-12 part, Radix Paeoniae Alba 3-15 part, Fructus Mume 5-20 part;
(2) wherein Radix Bupleuri, the Radix Paeoniae Alba, Fructus Aurantii Immaturus, Radix Et Rhizoma Rhei, Radix Scutellariae, Rhizoma Coptidis are used the 75-90% alcohol reflux, reflux, extract, twice, each 10 times of medical material weight were extracted 1.5 hours at every turn, and extracting solution cools, filters, merges;
(3) add other medical materials in the medicinal residues, add the water reflux, extract,, the extracting solution concentrating under reduced pressure, it is 65-75% to containing pure weight that concentrated solution adds ethanol, filters;
(4) (2) and (3) gained filtrate is merged, be condensed into extractum.
2. the described preparation method of claim 1 is characterized in that in the step (2), concentration of alcohol is 80%.
3. the described preparation method of claim 1 is characterized in that in the step (3), reflux, extract, twice, and each 10 times of medical material weight were extracted 1.5 hours at every turn.
4. the described preparation method of claim 1 is characterized in that in the step (3), and containing pure weight is 70%.
Priority Applications (15)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100585725A CN101357181B (en) | 2007-08-02 | 2007-08-02 | Medicine containing bupleurum root for treating diabetes and preparation method thereof |
| CA2687492A CA2687492C (en) | 2007-08-02 | 2008-07-28 | A herbal composition, method for preparing the same and use thereof for treating diabetes mellitus |
| DK08783574.0T DK2172207T3 (en) | 2007-08-02 | 2008-07-28 | Pharmaceutical composition for the treatment of diabetes and its method of preparation |
| US12/601,236 US8637092B2 (en) | 2007-08-02 | 2008-07-28 | Pharmaceutical composition for treating diabetes and its process for preparation |
| MYPI20094878A MY154567A (en) | 2007-08-02 | 2008-07-28 | A pharmaceutical composition for treating diabetes and its process for preparation |
| EP08783574A EP2172207B1 (en) | 2007-08-02 | 2008-07-28 | A pharmaceutical composition for treating diabetes and its process for preparation |
| NZ581525A NZ581525A (en) | 2007-08-02 | 2008-07-28 | A pharmaceutical composition for treating diabetes and its process for preparation |
| AU2008281227A AU2008281227B2 (en) | 2007-08-02 | 2008-07-28 | A pharmaceutical composition for treating diabetes and its process for preparation |
| PCT/CN2008/001383 WO2009015557A1 (en) | 2007-08-02 | 2008-07-28 | A pharmaceutical composition for treating diabetes and its process for preparation |
| KR1020107003490A KR101462984B1 (en) | 2007-08-02 | 2008-07-28 | A pharmaceutical composition for treating diabetes and its process for preparation |
| JP2010518477A JP5508264B2 (en) | 2007-08-02 | 2008-07-28 | PHARMACEUTICAL COMPOSITION FOR DIABETES TREATMENT AND METHOD FOR PRODUCING THE SAME |
| BRPI0812792A BRPI0812792B8 (en) | 2007-08-02 | 2008-07-28 | method for preparing a pharmaceutical composition for the treatment of diabetes mellitus and said pharmaceutical composition |
| RU2009144878/15A RU2485968C2 (en) | 2007-08-02 | 2008-07-28 | Drug preparation for treating diabetes and method for preparing it |
| ARP080103309A AR070306A1 (en) | 2007-08-02 | 2008-07-30 | A PHARMACEUTICAL COMPOSITION TO TREAT MELLITUS DIABETES AND THE METHOD TO PREPARE THE SAME |
| HK10104520.9A HK1138206A1 (en) | 2007-08-02 | 2010-05-10 | A pharmaceutical composition for treating diabetes and its process for preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100585725A CN101357181B (en) | 2007-08-02 | 2007-08-02 | Medicine containing bupleurum root for treating diabetes and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101357181A CN101357181A (en) | 2009-02-04 |
| CN101357181B true CN101357181B (en) | 2011-09-21 |
Family
ID=40329867
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007100585725A Active CN101357181B (en) | 2007-08-02 | 2007-08-02 | Medicine containing bupleurum root for treating diabetes and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101357181B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105943860A (en) * | 2016-06-21 | 2016-09-21 | 青岛辰达生物科技有限公司 | Medicine preparation for treating diabetes mellitus and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1376495A (en) * | 2002-01-31 | 2002-10-30 | 刘惠生 | Chinese medicine for treating diabetes and its complication |
| CN1726929A (en) * | 2004-07-30 | 2006-02-01 | 天津天士力制药股份有限公司 | Compsn. of medication for treating diabetes |
-
2007
- 2007-08-02 CN CN2007100585725A patent/CN101357181B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1376495A (en) * | 2002-01-31 | 2002-10-30 | 刘惠生 | Chinese medicine for treating diabetes and its complication |
| CN1726929A (en) * | 2004-07-30 | 2006-02-01 | 天津天士力制药股份有限公司 | Compsn. of medication for treating diabetes |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101357181A (en) | 2009-02-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102274447B (en) | Medicinal composition for treating diabetes | |
| CN101947284B (en) | Traditional Chinese medicinal composition for treating diabetes and preparation method thereof | |
| CN103223111B (en) | Traditional Chinese medicine composition for treating diabetic nephropathy and preparation method thereof | |
| CN102885975B (en) | Traditional Chinese medicine composition for treating diabetes mellitus | |
| CN101732491B (en) | Medicament for treating hypertension, hyperlipoidemia, high blood viscosity and anti-aging | |
| CN104083640B (en) | Traditional Chinese medicinal composition for treating diabetes mellitus and preparation method thereof | |
| CN101041010A (en) | Medicine compound for treating diabetes and its preparation | |
| CN101607031B (en) | Medicated wine for curing and preventing diabetes mellitus, preparation method and application method thereof | |
| CN106474083A (en) | Peaceful no sugar chawing tablet and its preparation technology and the application of Fructus Lycii sugar | |
| CN101357183B (en) | Medicine containing citrus aurantium for treating diabetes and preparation method thereof | |
| CN101112440A (en) | Medicinal composition for treating intestinal obstruction and method for preparing the same | |
| CN101357181B (en) | Medicine containing bupleurum root for treating diabetes and preparation method thereof | |
| CN100548363C (en) | Medicine of treatment benign prostate hyperplasia and preparation method thereof | |
| CN102552806A (en) | Fat-burning dredging particles and preparation method thereof | |
| CN104547530A (en) | Chinese medicinal composition for treating diabetes | |
| CN101357182B (en) | Medicine containing dark plum for treating diabetes and preparation method thereof | |
| CN108283687A (en) | It is a kind of prevention hypertension and heart damage integration of drinking and medicinal herbs Chinese medicine composition and its application | |
| CN101234165B (en) | Pharmaceutical combination for treating asthma or rhinitis and preparation thereof | |
| CN100593411C (en) | Blood-nourishing soft Chinese angelica capsule and its preparation process | |
| CN1792372A (en) | Traditional Chinese medicine prepn. for treating diabetes | |
| CN101357180A (en) | Medicine containing trichosanthes root for treating diabetes and preparation method thereof | |
| CN1943755B (en) | A Chinese traditional medicinal composition for treatment of hypertension and its preparation method | |
| CN103520684B (en) | Traditional Chinese medicine compound for reducing blood sugar | |
| CN103989940A (en) | Traditional Chinese medicine composition for treating diabetes mellitus | |
| CN105727089A (en) | Application of medicine composition containing folium artemisiae argyi to preparing medicine for treating irritable bowel syndrome |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: TASLY PHARMACEUTICAL GROUP CO., LTD. Free format text: FORMER NAME: TIANJIN TIANSHILI PHARMACEUTICAL CO., LTD. |
|
| CP01 | Change in the name or title of a patent holder |
Address after: 300410 Tianjin City Hedong District of Beichen Puji Road No. 2 city of the modern Chinese Medicine Patentee after: Tasly Pharmaceutical Group Co., Ltd. Address before: 300410 Tianjin City Hedong District of Beichen Puji Road No. 2 city of the modern Chinese Medicine Patentee before: Tianjin Tianshili Pharmaceutical Co., Ltd. |
|
| CP03 | Change of name, title or address |
Address after: 300410 Tianjin city Beichen District Huaihe road and road intersection Dingjiang tianzhijiao Park forensic Center for Intellectual Property Department Patentee after: Tasly Pharmaceutical Group Limited by Share Ltd Address before: 300410 Tianjin City Hedong District of Beichen Puji Road No. 2 city of the modern Chinese Medicine Patentee before: Tasly Pharmaceutical Group Co., Ltd. |
|
| CP03 | Change of name, title or address | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220607 Address after: 101-1032, F1, building 3, No. 103, Beiqing Road, Haidian District, Beijing 100081 Patentee after: Beijing zhongyitang Technology Co.,Ltd. Address before: 300410 Tianjin Beichen District Huaihe road and Ting Jiang West Road intersection heaven proud of the park Legal Center Intellectual Property Department Patentee before: TASLY PHARMACEUTICAL GROUP Co.,Ltd. |
|
| TR01 | Transfer of patent right |