CN101348802A - Technological process for producing red pigment by integrated membrane system - Google Patents
Technological process for producing red pigment by integrated membrane system Download PDFInfo
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- CN101348802A CN101348802A CNA2007100292404A CN200710029240A CN101348802A CN 101348802 A CN101348802 A CN 101348802A CN A2007100292404 A CNA2007100292404 A CN A2007100292404A CN 200710029240 A CN200710029240 A CN 200710029240A CN 101348802 A CN101348802 A CN 101348802A
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Abstract
The invention provides a technological method for producing monascus red pigment by adoption of the whole membrane technology. The method comprises the following steps: a monascus strain is inoculated into the prior fermentation medium for liquid submerged fermentation; an ultrafiltration membrane or a micro-filtration membrane is used for filtering a fermentation broth most of which is removed, and then ethanol or hot water or another solvent is added into concentrated solution for leaching; an ultrafiltration membrane or a micro-filtration membrane is used for filtering a leaching liquor, and clarifying solution is obtained; a nano-filtration membrane is used for concentrating and removing partial water and ethanol or water; as for alcohol extraction of the monascus red pigment, concentrated feed is distilled and a small amount of ethanol is removed, and then finished products are obtained after mixture and spray drying of the concentrated feed; and as for water extraction of the monascus red pigment, the finished products are obtained after mixture and spray drying of the concentrated feed. The method selects the ultrafiltraiton membranes or the micro-filtration membranes with different filtration precisions to process the fermentation broth and the leaching liquor and uses the nano-filtration membrane to concentrate the clarifying solution instead of using plates and frames for filtration, dose not require adjusting the isoelectric point, eliminates the influence of chemical medicines, improves the product yield and the quality and the color value of the finished products, and has low energy consumption, low cost, high benefit and high application value.
Description
Technical field
The present invention relates to a kind of producing and manufacturing technique of monascorubin, especially a kind of processing method that adopts full embrane method to produce monascorubin.
Background technology
Monascorubin is a kind of multiple constituents mixt that contains.General raw product has multiple composition, and its main application is as edible red pigments.
Traditional solution fermentation monascorubin production technique such as Fig. 1 and shown in Figure 2, its technological process comprises: with traditional fermention medium, the inoculation monascus specie ventilates and carries out liquid submerged fermentation; Fermented liquid is crossed filter press after transferring iso-electric point, after filter residue washes with water, add a certain amount of 70%～80% ethanol or with after hot water lixiviate for some time, remove filter residue with pressure filter again, reclaim cleaner liquid, through distill concentrate after, reclaim ethanol or remove moisture, feed liquid is carried out spraying drying and is obtained the monascorubin finished product after batching.
This traditional processing technology exists following technical disadvantages with not enough:
1, before the filtering fermentation liquor, owing to compare thickness, directly very difficult with Plate Filtration, so need to add acid or alkali,, change the character of material to regulate the iso-electric point of liquid, allow to smooth filtration; But after adding acid or alkali, introduced chemical composition in the material, influenced the quality of postorder product, reduced finished product look valency.
2, the clear liquid concentration after lixiviate is filtered has only 2～3%, directly handles with underpressure distillation, and facility investment is big, and energy consumption is very high during operation.
3, when dealing with improperly, also can occur can be carcinogenic notalin (molecular weight 200～300), traditional technology can't be removed, and the quality problems that China's monascorubin the time runs in outlet are mainly exceeded standard by notalin and cause.
Summary of the invention
In order to overcome the technological deficiency that monascorubin look valency is low, of poor quality in the traditional processing technology, energy consumption is high, cost is big, the present invention proposes the processing method that a kind of productivity effect height, look valency height, quality is good, energy consumption the is low full embrane method of employing are produced monascorubin.
For achieving the above object, the present invention is achieved by the following technical programs:
A kind of processing method that adopts full embrane method to produce monascorubin, key step is:
1, fermentation culture: with the traditional zymotic substratum, the inoculation monascus specie carries out liquid submerged fermentation;
2, ultra-filtration membrane or micro-filtrate membrane filtration fermented liquid: the ultra-filtration membrane or the micro-filtrate membrane filtration that with the fermented liquid filtering accuracy are 0.05～0.2 micron.Select for use organic or inorganic ultra-filtration membrane or microfiltration membrane directly fermented liquid to be carried out filtration treatment, remove most mother liquor, reclaim the raw material of concentrated solution as lixiviate.Preferentially selecting filtering accuracy during membrane filtration for use is 0.2 micron inorganic ceramic micro-filtrate membrane filtration, and also can select filtering accuracy for use is 0.05～0.2 micron organic hollow cellulosic ultrafiltration film or microfiltration membrane, perhaps selects organic rolling ultra-filtration membrane or micro-filtrate membrane filtration for use.
3, lixiviate: after removing the mother liquor of most of fermentation, concentrated solution is with ethanol or use hot water, broken skin lixiviate for some time.For the alcohol extracting monascorubin, with the broken skin lixiviate of ethanol of 70%～80% concentration; And carry monascorubin for water, then with the broken skin lixiviate of 80～100 ℃ of hot water; Also available other solvents carry out lixiviate.
4, ultra-filtration membrane or micro-filtrate membrane filtration vat liquor: remove filter residue in the vat liquor with ultra-filtration membrane or microfiltration membrane, obtain cleaner liquid;
Select for use organic or inorganic ultra-filtration membrane or microfiltration membrane directly vat liquor to be carried out filtration treatment, obtain cleaner liquid, and a small amount of dope after concentrating reclaims cleaner liquid with conventional filtration again, the filter residue reject, the clear liquid of membrane filtration and the clear liquid of conventional filtration merge as the product clear liquid.Preferentially selecting filtering accuracy during membrane filtration for use is 0.05 micron inorganic ceramic ultrafiltration membrance filter, and also can select filtering accuracy for use is 0.05～0.2 micron organic hollow cellulosic ultrafiltration film or microfiltration membrane, perhaps organic rolling ultra-filtration membrane or micro-filtrate membrane filtration.
5, nanofiltration membrane concentrates: cleaner liquid concentrates with nanofiltration membrane, removes most water and ethanol (perhaps water).Preferentially select for use organic nanofiltration membrane of molecular weight cut-off 100～300 that clear liquid is concentrated, for seeing through in the liquid with the monascorubin of ethanol-extracted mainly is to remove most water and ethanol, mainly is to remove most water and see through in the liquid for the monascorubin of water extraction.Both all can most ofly remove the notalin that influences quality product, and both all can be back to use in the leaching process through liquid simultaneously.Concentrated solution after nanofiltration membrane concentrates enters the back operation and handles.
6, aftertreatment: (distillation concentrates) → batching → spraying drying → finished product
Concentrate feed for the monascorubin of using alcohol extracting will be removed a spot of ethanol again through distillation, carries out spraying drying after batching, promptly obtains the finished product of monascorubin.Directly carry out spraying drying after the concentrated solution of the monascorubin of carrying for water is then prepared burden, obtain the finished product of monascorubin.
The present invention compares with traditional technology, has following obvious improvement and outstanding characteristics:
1, adopt full embrane method to produce monascorubin, the monascorubin quality height of production.Selecting filtering accuracy in the technology for use is that 0.05～0.2 micron ultra-filtration membrane or micro-filtrate membrane filtration are handled fermented liquid, replace Plate Filtration, need not regulate iso-electric point, therefore eliminated the influence of pharmaceutical chemicals product, favourable to reclaiming pigment, improved final product quality and finished product look valency.
2, vat liquor selects for use ultra-filtration membrane or microfiltration membrane to filter, good filtration effect on the one hand, and the product yield height adopts film to handle vat liquor on the other hand, to concentrating the effect of getting good protective membrane with nanofiltration membrane, effectively prolongs nanofiltration membrane work-ing life.
3, production energy consumption is low, and cost is few, the efficient height.Select for use the nanofiltration membrane of molecular weight cut-off 100～300 to carry out concentrating of cleaner liquid, can be concentrated to 20～30% concentration, with after the underpressure distillation removal small amount of ethanol, can carry out spraying drying again from 2～3% concentration.Treatment capacity during underpressure distillation will reduce about 10 times than traditional technology, and facility investment significantly reduces; And nanofiltration membrane concentrates and to carry out at low temperatures, no phase transformation, and no chemical transformation can effectively be removed inorganic salt, the ash content in the minimizing finished product; Because the spissated energy consumption of underpressure distillation is about 0.8～0.6 ton of steam/ton water, cracking-off removes about 80～120 yuan of water cost per ton, and when concentrating with nanofiltration, only with the about 20～30kwh/ ton of electric energy water, cracking-off only needs about 20～30 yuan except that water cost per ton.Compare with traditional technology, greatly reduce energy consumption and cost.
4, solved the bottleneck problem that notalin exceeds standard.Because the molecular weight of monascorubin is about 350, and the molecular weight of notalin is 200～300, so carrying out the spissated while with nanofiltration membrane, can select the membrane element of molecular weight cut-off 100～300 for use, make notalin see through film, the purifying pigment impels the notalin contents level in the finished product to reach standard level, thoroughly solves the quality problems of China's monascorubin outlet.
5, finished product look valency height.It is high about 10～40% that the finished product look valency that the monascorubin look price ratio traditional technology of producing with full embrane method production technique is produced is wanted, and improved final product quality.
Description of drawings
The present invention is further illustrated below in conjunction with accompanying drawing and embodiment.
Fig. 1 is the technological process of production figure of traditional monascorubin ethanol lixiviate.
Fig. 2 is the technological process of production figure of traditional monascorubin hot water lixiviate.
Fig. 3 is the technology realization flow figure of the embodiment of the invention one.
Fig. 4 is the technology realization flow figure of the embodiment of the invention two.
Embodiment
Embodiment one:
Fig. 3 shows the technological process of production figure of the embodiment of the invention one alcohol extracting monascorubin.As shown in Figure 3, with the traditional zymotic substratum, the inoculation monascus specie carries out liquid submerged fermentation; It with the fermented liquid filtering accuracy 0.2 micron inorganic ceramic micro-filtrate membrane filtration; After removing the mother liquor of most of fermentation, add broken skin lixiviate for some time of ethanol of a certain amount of 70%～80% concentration in the concentrated solution; Be that 0.05 micron inorganic ceramic ultrafiltration membrance filter obtains seeing through clear liquid with filtering accuracy again, and a small amount of dope reclaims cleaner liquid with conventional filtration again, filter residue reject, the clear liquid of membrane filtration and the clear liquid of conventional filtration merge as the product clear liquid; The cleaner liquid molecular weight cut-off is that organic nanofiltration membrane of 200 concentrates, and removes most water and ethanol; See through liquid and can be back to use in the leaching process, the concentrated solution after nanofiltration membrane concentrates distills, and removes a spot of ethanol, carries out spraying drying again after batching, promptly obtains the finished product of monascorubin.
Embodiment two:
Fig. 4 shows the technological process of production figure that the embodiment of the invention two water are carried monascorubin.As shown in Figure 4, with the traditional zymotic substratum, the inoculation monascus specie carries out liquid submerged fermentation; It with the fermented liquid filtering accuracy 0.2 micron inorganic ceramic micro-filtrate membrane filtration; After removing the mother liquor of most of fermentation, add broken skin lixiviate for some time of a certain amount of 80～100 ℃ hot water in the concentrated solution; Be that 0.05 micron inorganic ceramic ultrafiltration membrance filter obtains clear liquid with filtering accuracy again, and a small amount of concentrated solution reclaims cleaner liquid with conventional filtration again, filter residue reject, the clear liquid of membrane filtration and the clear liquid of conventional filtration merge as the product clear liquid; Cleaner liquid concentrates with organic nanofiltration membrane of molecular weight cut-off 200 again, removes most water; See through liquid and can be back to use in the leaching process, carry out spraying drying after the concentrated solution after nanofiltration membrane concentrates enters batching, promptly obtain the finished product of monascorubin.
According to above embodiment, just can realize the object of the invention preferably.
The foregoing description is only enumerated for explanation the present invention, is not to be used to limit the present invention, and the method for any effects equivalent based on the conversion of the technical program institute all belongs to protection scope of the present invention.
Claims (8)
1, a kind of processing method that adopts full embrane method to produce monascorubin is characterized in that: may further comprise the steps
A, with the traditional zymotic substratum, the inoculation monascus specie, carry out liquid submerged fermentation;
B, fermented liquid filter with ultra-filtration membrane or microfiltration membrane;
C, remove the mother liquor of most of fermentation after, add the ethanol of a certain amount of 70%～80% concentration in the concentrated solution, or, break skin lixiviate for some time with 80～100 ℃ hot water;
D, the filter residue of removing in the vat liquor with ultra-filtration membrane or micro-filtrate membrane filtration obtain cleaner liquid;
E, cleaner liquid concentrate with nanofiltration membrane, removing most water and ethanol, or remove most water;
F, will concentrate feed liquid earlier after distillation is removed small amount of ethanol and prepared burden, carry out spraying drying again, promptly obtain the monascorubin finished product with the monascorubin of ethanol lixiviate; To concentrate with the monascorubin of hot water lixiviate and carry out spraying drying again after feed liquid is prepared burden, promptly obtain the monascorubin finished product.
2, a kind of processing method that adopts full embrane method to produce monascorubin according to claim 1, it is characterized in that: ultra-filtration membrane that the described filtering fermentation liquor of step b is used or microfiltration membrane are organic hollow cellulosic ultrafiltration film or microfiltration membrane, or organic rolling ultra-filtration membrane or microfiltration membrane, or inorganic ultra-filtration membrane or microfiltration membrane.
3, a kind of processing method that adopts full embrane method to produce monascorubin according to claim 1 and 2, it is characterized in that: the ultra-filtration membrane that described filtering fermentation liquor is used or the filtering accuracy of microfiltration membrane are 0.05～0.2 micron.
4, a kind of processing method that adopts full embrane method to produce monascorubin according to claim 3, it is characterized in that: the microfiltration membrane that described filtering fermentation liquor is used is that filtering accuracy is 0.2 micron an inorganic ceramic microfiltration membrane.
5, a kind of processing method that adopts full embrane method to produce monascorubin according to claim 1, it is characterized in that: ultra-filtration membrane or microfiltration membrane that the described vat liquor of steps d filters usefulness are organic hollow cellulosic ultrafiltration film or microfiltration membrane, or organic rolling ultra-filtration membrane or microfiltration membrane, or inorganic ultra-filtration membrane or microfiltration membrane.
6, a kind of according to claim 1 or 5 processing method that adopts full embrane method to produce monascorubin, it is characterized in that: ultra-filtration membrane or micro-filtrate membrane filtration precision that described vat liquor filters usefulness are 0.05～0.2 micron.
7, a kind of processing method that adopts full embrane method to produce monascorubin according to claim 6, it is characterized in that: the ultra-filtration membrane that described vat liquor filters usefulness is that filtering accuracy is 0.05 micron an inorganic ceramic ultra-filtration membrane.
8, a kind of processing method that adopts full embrane method to produce monascorubin according to claim 1, it is characterized in that: the described nanofiltration membrane of step e is that molecular weight cut-off is organic nanofiltration membrane of 100～300.
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| CNA2007100292404A CN101348802A (en) | 2007-07-19 | 2007-07-19 | Technological process for producing red pigment by integrated membrane system |
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| CNA2007100292404A CN101348802A (en) | 2007-07-19 | 2007-07-19 | Technological process for producing red pigment by integrated membrane system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107189490A (en) * | 2017-07-07 | 2017-09-22 | 福建正味生物科技有限公司 | A kind of preparation method of water-soluble High color values monascorubin |
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- 2007-07-19 CN CNA2007100292404A patent/CN101348802A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107189490A (en) * | 2017-07-07 | 2017-09-22 | 福建正味生物科技有限公司 | A kind of preparation method of water-soluble High color values monascorubin |
| CN107189490B (en) * | 2017-07-07 | 2019-06-14 | 福建正味生物科技有限公司 | A kind of preparation method of high water solubility color value monascorubin |
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Owner name: GUANGZHOU ZHONGKE JIANYU WATER TREATMENT TECHNOLOG Free format text: FORMER OWNER: GUANGZHOU YING NA MO TECHNOLOGY CO., LTD. Effective date: 20090918 |
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Effective date of registration: 20090918 Address after: No. 18, research road, Science City, Guangdong, Guangzhou Province, China: 510000 Applicant after: Guangzhou Great Water Water Treatment Technology Co., Ltd. Address before: Guangdong city of Guangzhou province Tianhe District five mountain Wushan Technology Square C532 real post encoding: 510630 Applicant before: Guangzhou Innovation Mernbrane Technology Co., Ltd. |
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Open date: 20090121 |