CN101344526A - Fast and accurate prostatic cancer detection test paper strip and its preparation and application - Google Patents
Fast and accurate prostatic cancer detection test paper strip and its preparation and application Download PDFInfo
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Abstract
The invention discloses a test strip that is used for quickly and precisely detecting prostatic cancer, as well as the preparation and the application of the test strip, belonging to the technical field of biology. The invention uses a colorized nano microsphere as a marker for respectively marking PSA antibody and AMACR, combines an AMACR auto-antibody test method with an existing PSA immune detection method, sets up a quick two-valued detecting system of biological marker molecules, adopts an appropriative immune chromatography component, and consequently produces the two-valued test strip for the immune chromatography detection. Compared with existing prostatic cancer diagnosis methods in the current domestic market that detect PSA concentration in human blood, the test strip has the advantages that the utilized two-valued detecting system has greatly-raised sensitivity and specificity in the prostatic cancer detection, which are respectively 83.3 percent and 86.7 percent.
Description
Technical field
A kind of test strip of prostate cancer fast, accurately and preparation thereof and application the invention belongs to biological technical field.
Background technology
Statistics shows that the U.S. male sex has 179,000 people to be diagnosed as prostate cancer every year, and mortality ratio approximately is 25%.The case of the annual newly-increased prostate cancer of China is 70,000-80,000 people.The mortality ratio of advanced prostate cancer is very high, if but find early, prostate cancer patient can obtain very high five year survival rate.Thereby, very important to the early diagnosis of prostate cancer.The diagnosis of tradition prostate cancer relies on pathological examination, need get biological tissue, brings misery and inconvenience to patient.PSA (prostate specific antigen) is a kind of proteinase that only is found in male prostate.At present, in the blood PSA concentration as the important indicator of prostate cancer.But its sensitivity and specificity are all very low, can not be as the usefulness of extensive diagnosis generaI investigation.So the research of new tumor markers is the key areas of tumor research always.[list of references: Ma Le, Wu Xiaojing, Ran Jun; History, present situation and the development of the research of prostate cancer tumor marker, Chinese laboratory medicine magazine the 24th volume fourth phase of July calendar year 2001].
AMACR (Alpha-Methyl acyl-CoA racemase) is a kind of racemase that current research is found, expression is significantly higher than the normal person in prostate cancer tissue, can be used as the diagnosis index of early prostate cancer pathology.The advantage of AMACR is that it is a cancer specific, only is present in cancer tissue.AMACR also can be used as other cancers, for example the diagnosis marker of colorectal cancer, oophoroma, breast cancer, carcinoma of urinary bladder, lung cancer, lymthoma and melanoma or the like.Express the highest with colorectal cancer and prostate cancer.But the concentration of AMACR in blood is too low, is not suitable for doing separately prostate cancer marker.Up-to-date clinical research shows that AMACR causes the increase of anti-AMACR antibody in the blood, produces tangible anti-AMACR autoantibody in prostate cancer patient's blood sample.As detect anti-AMACR antibody in the blood, and the sensitivity that detects prostate cancer is 77%, specificity is 80% (p＜0.001), is better than PSA far away.[list of references: Journal of theNational Cancer Institute, Vol.96, No.11, June 2,2004]
Immunochromatography (Immunochromatography IC) is a kind of tachysynthesis analytical technology that early eighties grows up.Its principle is: by capillary action, sample swimming on the film that fibre strip is made, the part in certain zone combines on determinand wherein and the film, and by enzyme-catalytic chromogenic reaction or directly use colored marker, the short time, (in the 20min) just can obtain visualized result.It must not carry out separating of binding label and free label, has saved loaded down with trivial details application of sample, washing step, thereby simple to operate, and fast, personnel need not train, and does not need or only need simple instrument.Be fit to very much the usefulness of on-the-spot detection, also be applicable to the self-monitoring of chronic patient and the self health care of household person.
At present commonly used in the immunochromatography is that the Fast Detection Technique of label exists significant limitation with the collaurum, can only be combined in the surface of bronze by absorption such as its antibody.Under many circumstances, this physisorption is illusive, and some antibody is difficult to be adsorbed on the bronze surface especially, and the antibody that perhaps is adsorbed on the bronze surface may come off and cause the failure that detects.Secondly, another limitation of golden target is its solid color, is difficult to multiple detection.And golden mark method relies on its distinctive brown testing result that shows, can't come reading to improve the sensitivity that detects and to carry out quantitative test with the instrument of similar luminoscope.The appearance of biological nano technology provides a good solution route for this reason.Visible light and the fluorescent nanometer microsphere series of products produced on the at present domestic and international macromolecule chemical industry market comprise multiple colors such as red, blue, green, yellow, purple, black, with liquid antibody chip (Luminex) technology type seemingly, these color nano microballoons tens kinds of different antibody that can be used for encoding, thus realize the fast detecting of multiple mark.In addition, adopt fluorescent nanometer microsphere to replace bronze can improve the sensitivity of detection greatly, can carry out quantitative test simultaneously.The more important thing is, these Nano microsphere surfaces contain-functional group of COOH or other kinds, and can carrying out covalence key with the reactive group on the various antibody, these functional groups combine, form stable antibody-microballoon conjugated body.In addition, these microspheres products also have stability, repeatability, monodispersity, be applicable to large-scale production, good characteristic such as operation facility.The detection that nanometer technology is used for the disease biomarker at home and abroad also just begins at present.Can predict, replace the colloid bronze with visible light and fluorescent nanometer microsphere, set up the multiple immunochromatographiassays assays platform of multivalence, be this development trend in several years.
Summary of the invention
First purpose of the present invention: chromatograph test strip that quick accurate detecting method adopted of prostate cancer and preparation method thereof is provided.Second purpose of the present invention: provide this chromatograph test strip to be applied in the quick accurate detecting method of prostate cancer.The present invention is the certification mark thing with PSA and AMACR autoantibody, with color nano microballoon difference mark PSA antibody and AMACR, sets up divalence immunochromatography detection system.The present invention compares with the detection method of present traditional prostate cancer, have consuming time less, high specificity, highly sensitive, do not need characteristics such as large-scale instrument and equipment, be particularly useful for the usefulness that generaI investigation was used or diagnosed on a large scale in vast rural area, County Hospital, clinic, community.
Technical scheme of the present invention:
1, a kind of test strip of prostate cancer, by PVC base plate (1), glass fibre membrane application of sample pad (2), pad (3), nitrocellulose filter (4) and adsorptive pads (5) are formed, put into wire or banded mouse-anti human IgG detection antibody detection line one (6) on nitrocellulose filter (4) successively, PSA detects antibody detection line two (7), and beta-actin detects antibody control line (8).
2, the preparation method of described test strip:
(1) select carrier: selected carrier is the color nano microballoon of particle diameter at 10～400nm, must contain on this carrier microballoons to supply the immune aglucon of coupling: carboxyl, hydroxyl, amino or aldehyde radical;
(2) preparation immune microsphere: with anhydrous morpholino b acid (MES) the color nano microballoon is carried out pre-service earlier, with a spot of carbodiimides (EDC), and N-hydroxy-succinamide (NHS), add in the microspheres solution concussion activation microballoon on oscillator; Use the anti-people PSA of black nano microballoon coupling antibody, blue Nano microsphere coupling AMACR antigen respectively, with the anti-people's beta-actin of orange Nano microsphere coupling antibody as confidential reference items; There is not the activated group of complete reaction to seal three kinds of immune nano microsphere surfaces then, with contingent non-specific adsorption in the test after being reduced in; Microballoon is resuspended in again and preserves in the liquid, make the microspheres solution that concentration is 2mg/mL, preserve, the prescription of preserving liquid is: pH 9.0, contain 0.05%Tween-20,0.1%NaN
3The 0.005M borate buffer system of (Sodium Azide) and 0.1%BSA; To deceive at last, blue and three kinds of quality such as colored immune nano microballoon of orange are mixed and made into a kind of black Blue Curacao three mixture of colours immune nano microballoons;
(3) structure of test strip:
The bag quilt of antibody: on nitrocellulose membrane, with the amount of 1 μ L/cm the mouse-anti human IgG monoclonal anti liquid solution point of 2mg/mL is become wire or banded detection line one with a film device, antibody is dissolved in 0.15M, the pH7.4 phosphate buffer in advance, and containing the sucrose amount in the solution is 1%; With same method again on the mark PSA detect antibody detection line two and beta-actin detects the antibody control line, be put in dry 37 ℃ of airtight baking ovens baking then 30 minutes, be put in the drying basin stand-by after the taking-up;
Constitute pad: quality such as black, the blue and orange colored immune nano microballoon that will prepare are mixed and made into a kind of black Blue Curacao three mixture of colours immune nano microballoons, is that the mixed three look immune nano microballoons of 2mg/mL drop on the glass fibre membrane with liquid-transfering gun with 10～40 μ L, concentration, and dry, constitute pad;
The establishment of test strips: each parts of chromatograph test strip are superimposed: superimposed successively glass fibre membrane application of sample pad (2) on PVC base plate (1), pad (3), nitrocellulose filter (4) and adsorptive pads (5) are cut into the test strip of 75mm * 3mm bar shaped then.
The color nano microballoon carries out pre-service: with pH 5.0, contain the anhydrous morpholino b acid of 0.01M-tween solution MEST of 0.05%Tween-20, as activation buffer solution, get the blue Nano microsphere of 2mg in the 2mL centrifuge tube, add the washing of 500 μ L activation damping fluid: on the vortex oscillation device, mix, left the heart 30 minutes with per minute 13000 again, supernatant is outwelled; After adding twice of the same again operated wash microballoon of 500 μ L activation damping fluid, add in microballoon that 485 μ l activation damping fluid adds carbodiimides again and concentration is each 2.5mg of N-hydroxy-succinamide of 0.25g/mL, on the vortex oscillation device, mix, behind the room temperature reaction 30 minutes, remove unreacted activator with the same operated wash of MEST activation damping fluid, change centrifuge tube again, and activate twice of the same operated wash microballoon of damping fluid with MEST.
Prepare colored immune nano microballoon:
A), with AMACR and blue Nano microsphere coupling
Coupling: with 500 μ L, pH 9.0, contain the borate tween damping fluid (BST) of the 0.005M of 0.05%Tween-20, solution is made twice of the same operated wash microballoon of coupling buffer; Add the microballoon after the resuspended washing of 475 μ L coupling buffers, add AMACR (market is purchased) room temperature reaction 3 hours of 150 μ g again, AMACR is coupled to microsphere surface;
B), with PSA antibody and the coupling of black nano microballoon
AMACR changed into use PSA, blue Nano microsphere is changed into use the black nano microballoon, all the other are operated with step A;
C), beta-actin is detected antibody and orange Nano microsphere coupling
AMACR changed into use beta-actin, blue Nano microsphere is changed into use orange Nano microsphere, all the other are operated with step A.
The sealing of immune nano microballoon: the 1% bovine serum albumin(BSA) BSA that adds 500 μ L, BSA is dissolved in the borate tween damping fluid in advance, there is not the activated group of complete reaction to seal to the immune nano microsphere surface, and physisorption by BSA, seal other site, space, with contingent non-specific adsorption in the test after being reduced in, capping is 30 minutes under the room temperature.
The preservation of immune nano microballoon: with the immune microsphere after the sealing of the same operated wash of BST four times, at last microballoon is resuspended in and preserves in the liquid, make the microspheres solution that concentration is 2mg/mL, preserve, the prescription of preserving liquid is: pH 9.0, contain 0.05%Tween-20,0.1%NaN
30.005M borate buffer system with 0.1%BSA.
3, the application of described test strip, the inspection system method that this test strip is used for prostate cancer is: 1～100 μ L blood sample to be measured is dropped in carry out chromatography on the sample pad, room temperature specific band can occur after 3～5 minutes, the result judges: control line shows orange, the positive detection of promptly anti-people's beta-actin is effective, otherwise invalid for detecting; Detection line one and detection line two develop the color simultaneously, and it is positive that promptly specific band all appears in AMACR antibody and PSA, otherwise negative.
Beneficial effect of the present invention:
1, quick: all testing process only needs 3-20 minute;
2, easy: as not need other any instrument and equipment, operate also extremely simple;
3, easily store: the used color nano polymeric microspheres stabilize of the present invention is good, and the immune microsphere proterties of making is stable;
4, sensitivity and specificity height: the divalence detection system of utilization of the present invention all is greatly improved on sensitivity and specificity with respect to the method that only detects PSA or AMACR autoantibody.
The present invention combines the detection method of AMACR autoantibody with the immunologic detection method of existing P SA, set up one two valency biomarker molecule speed examining system, check in the blood PSA in the AMACR antibody and blood simultaneously, thereby detect AMACR or PSA improves a lot on sensitivity and specificity than individual event.In the generaI investigation of prostate cancer, we wish the specificity height, and false positive rate is low, utilize the present invention can guarantee low-down false positive rate.When large-scale prostate cancer is generally investigated, unnecessary psychology and burden on society be can not cause like this, or unnecessary subsequent examination and medical insurance burden caused.
Description of drawings
The test strip synoptic diagram of Fig. 1 prostate cancer.1, PVC base plate, 2, the application of sample pad, 3, pad, 4, nitrocellulose filter, 5, adsorptive pads, 6, the mouse-anti human IgG detects antibody (detection line one), 7, PSA detects antibody (detection line two), 8, beta-actin detection antibody (control line).
Embodiment
1. activation: with anhydrous morpholino b acid-tween solution (MEST of pH 5.0,0.01M, 0.05%Tween-20) as activation buffer solution, get the blue Nano microsphere of 2mg in the 2mL centrifuge tube, add the washing of 500 μ L activation damping fluid: on the vortex oscillation device, mix, left the heart 30 minutes with per minute 13000 again, supernatant is outwelled; After adding twice of the same again operated wash microballoon of 500 μ L activation damping fluid, add in microballoon that 485 μ l activation damping fluid adds carbodiimides again and concentration is each 2.5mg of N-hydroxy-succinamide of 0.25g/mL, on the vortex oscillation device, mix, behind the room temperature reaction 30 minutes, remove unreacted activator with the same operated wash of MEST activation damping fluid, change centrifuge tube again, and activate twice of the same operated wash microballoon of damping fluid with MEST.
2. coupling: with the borate tween damping fluid of 500 μ L, pH 9.0,0.005M (BST, 0.05%Tween-20) solution is made twice of the same operated wash microballoon of coupling buffer; Add the microballoon after the resuspended washing of 475 μ L coupling buffers, add AMACR (market is purchased) room temperature reaction 3 hours of 150 μ g again, AMACR is coupled to microsphere surface.
3. sealing: 1% bovine serum albumin(BSA) (BSA) (BSA is dissolved in the borate tween damping fluid in advance) that adds 500 μ L does not have the activated group of complete reaction to seal to the immune microsphere surface, and physisorption by BSA, seal other site, space, with contingent non-specific adsorption in the test after being reduced in, capping is 30 minutes under the room temperature.
4. preserve: with the immune microsphere after the same operated wash sealing of BST four times, microballoon is resuspended in preserves in the liquid at last, make the microspheres solution that concentration is 2mg/mL, the prescription of preserving liquid is: pH 9.0, contain 0.05%Tween-20,0.1%NaN
30.005M borate buffer system with 0.1%BSA.
AMACR changed into use PSA, blue Nano microsphere is changed into use the black nano microballoon, all the other are operated with embodiment 1.
Embodiment 3 detects antibody and orange Nano microsphere coupling with beta-actin
AMACR changed into use beta-actin, blue Nano microsphere is changed into use orange Nano microsphere, all the other are operated with embodiment 1.
The structure of embodiment 4 test strip
1. the bag quilt of antibody: on nitrocellulose membrane, with the amount of 1 μ L/cm the mouse-anti human IgG monoclonal anti liquid solution point of 2mg/mL is become wire or band shape (detection line one with a film device, see 6 among Fig. 1), antibody is dissolved in phosphate buffer (PBS in advance, 0.15M, pH7.4) in, cane sugar content is 1% in the solution; With same method again on the mark PSA detect antibody (detection line two is seen 7 among Fig. 1) and beta-actin detects antibody (control line is seen 8 among Fig. 1), be put in then in dry 37 ℃ of airtight baking ovens to toast 30 minutes, be put in the drying basin stand-by after the taking-up;
2. will deceive, quality such as blue and orange colored immune nano microballoon is mixed and made into a kind of black Blue Curacao three mixture of colours immune nano microballoons, is that the mixed three look immune nano microballoons of 2mg/mL drop on the glass fibre membrane with liquid-transfering gun with 10～40 μ L, concentration, and dry, constitute pad;
3. the establishment of test strips: each parts of chromatograph test strip are superimposed together by Fig. 1, and are cut into the test strip of 75mm * 3mm bar shaped.
The detection of embodiment 5 samples
1. 1～100 μ L blood sample to be measured is dropped in and carry out chromatography on the sample pad, room temperature specific band can occur after 3～5 minutes.
2. the result judges: the positive detection of anti-people's beta-actin just effectively (shows orange), otherwise invalid for detecting; If specific band positive (colour developing simultaneously) all appears in AMACR antibody and PSA, otherwise negative.
Carry out clinical detection with the test strip that said method is made, the result shows that the present invention compares with the detection method of traditional PSA, has higher sensitivity and specificity.
Get prostate cancer patient blood sample 30 examples, normal male blood sample 45 examples.
Utilize the method for the invention to detect, draw: true positives 25 examples, true negative 39 examples, false positive 6 examples, false negative 5 examples.
Utilize the detection method of traditional PSA to detect, draw: true positives 13 examples, true negative 23 examples, false positive 22 examples, false negative 17 examples.
Through sensitivity formula: true positives number/(true positives number+false negative number) * 100% and specificity formula: true negative number/(false positive number+true negative number) * 100% can draw:
Utilizing the present invention to detect prostate cancer sensitivity is 83.3%, and specificity is 86.7%;
Utilizing PSA to detect prostate cancer sensitivity is 43.3%, and specificity is 51.1%.
Claims (7)
1, a kind of test strip of prostate cancer, it is characterized in that by PVC base plate (1), glass fibre membrane application of sample pad (2), pad (3), nitrocellulose filter (4) and adsorptive pads (5) are formed, put into wire or banded mouse-anti human IgG detection antibody detection line one (6) on nitrocellulose filter (4) successively, PSA detects antibody detection line two (7), and beta-actin detects antibody control line (8).
2, the preparation method of the described test strip of claim 1 is characterized in that:
(1) select carrier: selected carrier is the color nano microballoon of particle diameter at 10～400nm, must contain on this carrier microballoons to supply the immune aglucon of coupling: carboxyl, hydroxyl, amino or aldehyde radical;
(2) preparation immune microsphere: with anhydrous morpholino b acid the color nano microballoon is carried out pre-service earlier, a spot of carbodiimides and N-hydroxy-succinamide are joined in the microspheres solution, concussion activation microballoon on oscillator; Use the anti-people PSA of black nano microballoon coupling antibody, blue Nano microsphere coupling AMACR antigen respectively, with the anti-people's beta-actin of orange Nano microsphere coupling antibody as confidential reference items; There is not the activated group of complete reaction to seal three kinds of immune nano microsphere surfaces then, with contingent non-specific adsorption in the test after being reduced in; Microballoon is resuspended in again and preserves in the liquid, make the microspheres solution that concentration is 2mg/mL, preserve, the prescription of preserving liquid is: pH 9.0, contain 0.05%Tween-20,0.1%NaN
30.005M borate buffer system with 0.1%BSA; To deceive at last, blue and three kinds of quality such as colored immune nano microballoon of orange are mixed and made into a kind of black Blue Curacao three mixture of colours immune nano microballoons;
(3) structure of test strip:
The bag quilt of antibody: on nitrocellulose membrane, with the amount of 1 μ L/cm the mouse-anti human IgG monoclonal anti liquid solution point of 2mg/mL is become wire or banded detection line one with a film device, antibody is dissolved in 0.15M, pH 7.4 phosphate buffers in advance, and containing the sucrose amount in the solution is 1%; With same method again on the mark PSA detect antibody detection line two and beta-actin detects the antibody control line, be put in dry 37 ℃ of airtight baking ovens baking then 30 minutes, be put in the drying basin stand-by after the taking-up;
Constitute pad: the quality such as black, blue and orange colored immune nano microballoon of step (2) preparation are mixed and made into a kind of black Blue Curacao three mixture of colours immune nano microballoons, is that the mixed three look immune nano microballoons of 2mg/mL drop on the glass fibre membrane with liquid-transfering gun with 10～40 μ L, concentration, and dry, constitute pad;
The establishment of test strips: each parts of chromatograph test strip are superimposed: superimposed successively glass fibre membrane application of sample pad (2) on PVC base plate (1), pad (3), nitrocellulose filter (4) and adsorptive pads (5) are cut into the test strip of 75mm * 3mm bar shaped then.
3, preparation method according to claim 2, it is characterized in that the color nano microballoon carries out pre-service, with pH 5.0, contain the anhydrous morpholino b acid of 0.01M-tween solution MEST of 0.05%Tween-20, as activation buffer solution, get the blue Nano microsphere of 2mg in the 2mL centrifuge tube, add the washing of 500 μ L activation damping fluid: on the vortex oscillation device, mix, left the heart 30 minutes with per minute 13000 again, supernatant is outwelled; After adding twice of the same again operated wash microballoon of 500 μ L activation damping fluid, add in microballoon that 485 μ l activation damping fluid adds carbodiimides again and concentration is each 2.5mg of N-hydroxy-succinamide of 0.25g/mL, on the vortex oscillation device, mix, behind the room temperature reaction 30 minutes, remove unreacted activator with the same operated wash of MEST activation damping fluid, change centrifuge tube again, and activate twice of the same operated wash microballoon of damping fluid with MEST.
4, preparation method according to claim 2 is characterized in that preparing colored immune nano microballoon:
A), with AMACR and blue Nano microsphere coupling
Coupling: make twice of the same operated wash microballoon of coupling buffer with 500 μ L, pH 9.0, the borate tween buffer B ST solution that contains the 0.005M of 0.05%Tween-20; Add the microballoon after the resuspended washing of 475 μ L coupling buffers, add the AMACR room temperature reaction 3 hours of 1501 μ g again, AMACR is coupled to microsphere surface;
B), with PSA antibody and the coupling of black nano microballoon
AMACR changed into use PSA, blue Nano microsphere is changed into use the black nano microballoon, all the other are operated with step A;
C), beta-actin is detected antibody and orange Nano microsphere coupling
AMACR changed into use beta-actin, blue Nano microsphere is changed into use orange Nano microsphere, all the other are operated with step A.
5, preparation method according to claim 2, it is characterized in that the sealing of immune nano microballoon: the 1% bovine serum albumin(BSA) BSA that adds 500 μ L, BSA is dissolved in the borate tween damping fluid in advance, there is not the activated group of complete reaction to seal to the immune nano microsphere surface, and physisorption by BSA, seal other site, space, with contingent non-specific adsorption in the test after being reduced in, capping is 30 minutes under the room temperature.
6, preparation method according to claim 2, it is characterized in that the preservation of immune nano microballoon: the immune microsphere after sealing with the same operated wash of BST four times, at last microballoon is resuspended in and preserves in the liquid, make the microspheres solution that concentration is 2mg/mL, the prescription of preserving liquid is: pH 9.0, contain 0.05%Tween-20,0.1%NaN
30.005M borate buffer system with 0.1%BSA.
7, the application of the described test strip of claim 1, it is characterized in that the inspection system method that this test strip is used for prostate cancer is: 1～100 μ L blood sample to be measured is dropped in carry out chromatography on the sample pad, room temperature specific band can occur after 3～5 minutes, the result judges: control line shows orange, the positive detection of promptly anti-people's beta-actin is effective, otherwise invalid for detecting; Detection line one and detection line two develop the color simultaneously, and it is positive that promptly specific band all appears in AMACR antibody and PSA, otherwise negative.
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| CN102072956A (en) * | 2010-12-20 | 2011-05-25 | 重庆市科学技术研究院 | Nano influenza virus testing paper |
| CN102326080A (en) * | 2009-02-20 | 2012-01-18 | 加利福尼亚大学董事会 | A+ biomarker assays |
| CN102539751A (en) * | 2010-12-09 | 2012-07-04 | 苏州生物医学工程技术研究所 | Immunofluorescence test paper strip and quantitative detection method thereof |
| CN103048467A (en) * | 2012-12-06 | 2013-04-17 | 苏州海吉亚生物科技有限公司 | Method for detecting tissue cell protein by using housekeeping protein |
| CN103364562A (en) * | 2013-08-01 | 2013-10-23 | 青岛宝依特生物制药有限公司 | Test strip for testing sulfanilamide drug residues and application method |
| CN103499691A (en) * | 2013-07-11 | 2014-01-08 | 苏州默锐克生物科技有限责任公司 | Detection test paper of HER2 protein expression level |
| CN107667290A (en) * | 2015-04-06 | 2018-02-06 | Blu诊断有限公司 | For detecting the detection means and application method of the analyte in saliva sample |
| CN114689849A (en) * | 2022-05-31 | 2022-07-01 | 上海尚济生物医学工程有限责任公司 | Antigen detection product and preparation method and application thereof |
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- 2008-08-22 CN CNA200810124502XA patent/CN101344526A/en active Pending
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| CN102326080A (en) * | 2009-02-20 | 2012-01-18 | 加利福尼亚大学董事会 | A+ biomarker assays |
| CN102326080B (en) * | 2009-02-20 | 2015-08-05 | 加利福尼亚大学董事会 | The test of A+ biomarker |
| US9354233B2 (en) | 2009-02-20 | 2016-05-31 | The Regents Of The University Of California | A+ biomarker assays |
| CN102539751A (en) * | 2010-12-09 | 2012-07-04 | 苏州生物医学工程技术研究所 | Immunofluorescence test paper strip and quantitative detection method thereof |
| CN102072956A (en) * | 2010-12-20 | 2011-05-25 | 重庆市科学技术研究院 | Nano influenza virus testing paper |
| CN103048467A (en) * | 2012-12-06 | 2013-04-17 | 苏州海吉亚生物科技有限公司 | Method for detecting tissue cell protein by using housekeeping protein |
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| CN103499691A (en) * | 2013-07-11 | 2014-01-08 | 苏州默锐克生物科技有限责任公司 | Detection test paper of HER2 protein expression level |
| CN103364562A (en) * | 2013-08-01 | 2013-10-23 | 青岛宝依特生物制药有限公司 | Test strip for testing sulfanilamide drug residues and application method |
| CN107667290A (en) * | 2015-04-06 | 2018-02-06 | Blu诊断有限公司 | For detecting the detection means and application method of the analyte in saliva sample |
| CN114689849A (en) * | 2022-05-31 | 2022-07-01 | 上海尚济生物医学工程有限责任公司 | Antigen detection product and preparation method and application thereof |
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