CN101341901B - Application of bdellovibrio in removing usual pathogen in fowls egg and meat - Google Patents
Application of bdellovibrio in removing usual pathogen in fowls egg and meat Download PDFInfo
- Publication number
- CN101341901B CN101341901B CN2008100301588A CN200810030158A CN101341901B CN 101341901 B CN101341901 B CN 101341901B CN 2008100301588 A CN2008100301588 A CN 2008100301588A CN 200810030158 A CN200810030158 A CN 200810030158A CN 101341901 B CN101341901 B CN 101341901B
- Authority
- CN
- China
- Prior art keywords
- bdellovibrio
- eggs
- meat
- beasts
- birds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Fodder In General (AREA)
Abstract
The invention discloses the application of bdellovibrio in removing the commonly seen pathogen in poultry, eggs and meat; the poultry, eggs and meat with pathogen are treated by bdellovibrio liquid in a spraying, rubbing or soaking way; when the soaking method is adopted, the concentration of the bdellovibrio at least reaches 10<2>puf/ml; when the spraying or the rubbing way is adopted and the use of bdellovibrio liquid with concentration equal to or more than 10<2>pfu/ml, the consumption at least reaches 15ml/kg. The disclosed two units of bdellovibrio can be used independently, and the effect after the two units are mixed is better. The application of the invention is convenient in use, safe, has no side effect, obvious effect in removing commonly seen pathogen in poultry, eggs and meat, germ clearing rate of more than 90 percent, is applicable to the whole process from cultivation to dining including poultry, egg and meat processing, directing improves the product quality, and verysignificant improving food safety and ensuring the health of human beings.
Description
Technical field
The invention belongs to biological technical field, relate to bacterium and application thereof, specifically be meant a kind of Bdellovibrio in the application of eliminating the common pathogen that birds, beasts and eggs and meat carries, be specially adapted to the elimination of common pathogen in the preservation of livestock and poultry cultivation environment, birds, beasts and eggs meat and the process thereof.
Background technology
Food security has become the common problem of paying close attention in the whole world, and as a global challenge is regarded food security by The World Health Organization (WHO).In recent years, global food security malignant event takes place frequently, and food origin disease has become the major issue that influences publilc health.According to the WHO statistics, developed country has 1/3rd people to infect food origin disease every year approximately, and some developing countries, food origin disease causes the main cause of people's unusual death often.Many-sided research and investigation result show that except that contingency and human factor, food origin disease mainly is caused by microorganism and chemicals.The statistics nearly 10 years according to Sino-U.S. CDC all accounts for about 50% of sum at the food origin disease that China and the United States are caused by microorganism, and the food origin disease that chemical substance causes accounts for the ratio of sum about the U.S. is 1%, about China is 27%.Various data both domestic and external and statistics show that microorganism is the arch-criminal who causes food origin disease.Over nearly 20 years, the animal husbandry and aquaculture of China has obtained swift and violent development, and it is increasing that its product accounts for the proportion of whole food service industry, and meat, breast, egg and aquatic products are the main kinds in the animal food.According to the statistics of national feed work office, China's meat products output in 2003 is that 6,920 ten thousand tons, birds, beasts and eggs amount are 2606.8 ten thousand tons, and the annual production of meat and eggs is and beats the world.But the annual export volume of China's meat only is more than 60 ten thousand tons, and less than 1% of total amount, the export volume of Poultry and Eggs is very little especially, and one of its reason is food-safety problems such as microbial contamination.Cause the microorganism ubiquity of food origin disease, but be not at every moment all can cause food origin disease, the relation that these pathogenic microorganisms and the non-pathogenic bacteria around it and environment exist mutual restriction, be in the poised state of little ecology, and after this balance is broken, the microorganism of causing a disease grows without restraint, thereby causes food origin disease to break out.The deterioration of China's environment and ecological destruction become increasingly conspicuous this problem.
The control of the pathogenic bacteria of carrying for birds, beasts and eggs and meat, at present also without any document announcement, existing data just takes some measures from the livestock and poultry cultivation process, for example: use antibiotic, to eliminate or to reduce the pathogenic bacteria that its live body carries, though this source measure or certain preventive effect is arranged has side effect to livestock and poultry, has directly influenced the quality of birds, beasts and eggs and meat.For example antibiotic abuse has caused pathogenic bacteria that antibiotic is developed immunity to drugs; Though the use of vaccine is an approach, immune effect is not obvious, and particularly people are in order to strengthen the immune effect of vaccine at present, and the strain of selecting for use is more and more stronger, and potential pathogenic risk is arranged on the contrary.Yet, Bdellovibrio has the special bacterium of cracking pathogenic bacteria as a class, not only may be used on livestock and poultry from culturing to the overall process before eating, and can not cause bacterium resistance to occur, also do not have the too single-minded shortcoming of host in the phagotherapy, so Bdellovibrio has suitable advantage as the biological removers of class pathogenic bacteria.
If can be before livestock and poultry be transported, eat or process from breed, animal products, carry out the biology elimination work of pathogenic bacteria earlier, then can reduce or eliminate the probability of food poisoning, the processing that particularly can be birds, beasts and eggs and meat product provides the facility in high-quality raw material and the processing.Therefore, utilize Bdellovibrio to eliminate pathogenic bacteria that birds, beasts and eggs and meat carries improving and to improve the security of birds, beasts and eggs and meat, the development of human health and China's birds, beasts and eggs and meat industry is had crucial meaning.
Summary of the invention
The objective of the invention is to overcome the drawback of prior art, find out by research and development and to be applicable to the Bdellovibrio of eliminating the common pathogen that birds, beasts and eggs and meat carries, thereby the Bdellovibrio that a kind of efficient, no side effects is provided is in the application of eliminating the common pathogen that birds, beasts and eggs and meat carries.
In order to achieve the above object, main technical schemes of the present invention is as follows:
A kind of Bdellovibrio is in the application of eliminating the common pathogen that birds, beasts and eggs and meat carries, and it is characterized in that: application process comprises following concrete steps and condition thereof:
Step 1: the separation and purification of Bdellovibrio
Water body or the bed mud of gathering carried out preliminary treatment, the Aeromonas hydrophila of inoculation propagation Bdellovibrio, fall double-layer plate, plaque appears on the double-layer plate that contains the host bacterium, single spot that picking constantly enlarges, cultivate by micro-biological process liquid propagation again, the double-layer plate check, single spot of constantly enlarging of picking once more, and repeated several times, to single plaque formation shape that goes down to posterity, size, the plaque that transparency is all consistent, be the pure bacterial strain of a strain Bdellovibrio, by the big subtotal of single spot in the same time, isolate the pure bacterial strain of 2 strain Bdellovibrios, described Bdellovibrio is being positioned at the Chinese typical culture collection center preservation of Chinese Wuhan City Wuhan University on January 13rd, 2008, Bdellovibrio sp.BDF01, deposit number are CCTCC NO:M 208008 and Bdellovibrio sp.BDF02, and deposit number is CCTCC NO:M 208009;
Step 2: the preparation of Bdellovibrio liquid
2 strain Bdellovibrios are carried out fermented and cultured respectively, and making concentration respectively is 10
2～10
12The Bdellovibrio liquid of pfu/ml, stand-by;
Step 3: Bdellovibrio is in the application of eliminating the common pathogen that birds, beasts and eggs and meat carries
The Bdellovibrio mixed liquor or the individual plant Bdellovibrio liquid that adopt above-mentioned 2 strain Bdellovibrios to mix by arbitrary proportion, method with sprinkling or obliterating or dipping is handled birds, beasts and eggs and the meat products that carries pathogenic bacteria, and wherein adopt the method for dipping: Bdellovibrio concentration reaches 10 at least
2Pfu/ml, its preferable range is 10
4～10
6Pfu/ml; Adopt the method for sprinkling or obliterating: working concentration 〉=10
2During the Bdellovibrio liquid of pfu/ml, be directly used in birds, beasts and eggs and meat products, its use amount reaches 15ml/kg at least, and preferred use amount scope is 35-55ml/kg; Be applied to environment, its use amount reaches 10ml/m at least
2
The optimum mixture ratio example of described Bdellovibrio mixed liquor is: BDF01: BDF02 is 1: 1.
Described applied environment comprises livestock and poultry farm breeding environment, preservation environment and birds, beasts and eggs and meat products means of transport, process.
When described application process is used for the elimination of livestock and poultry farm breeding environment pathogenic bacteria, adopt the method for spraying: when working concentration 〉=10
2During the Bdellovibrio liquid of pfu/ml, preferred use amount scope is 35～55ml/m
2
When described application process is used for the elimination of birds, beasts and eggs and meat curing or transportation environment pathogenic bacteria, adopt the method for spraying: when working concentration 〉=10
2During the Bdellovibrio liquid of pfu/ml, preferred use amount scope is 30～50ml/m
2
The present invention compared with prior art has following outstanding advantage and significant effect:
1, it is remarkable to adopt this law to eliminate the effects of the pathogenic bacteria that birds, beasts and eggs and meat carries
Should be with having overcome the side effect that the antibiotic abuse brings, also overcome the not obvious and too single-minded shortcoming of host in the phagotherapy of immune effect of vaccine, provide a kind of new method for eliminating birds, beasts and eggs and the entrained food-borne pathogens of meat, this method is applicable to the overall process that comprises the birds, beasts and eggs meat packing from " culturing dining table ".
When (1) being used for the elimination of livestock and poultry farm breeding environment pathogenic bacteria, can significantly reduce the quantity of the pathogenic bacteria in the breeding environment, can prevent or reduce the illness rate of livestock and poultry animal, thereby improve the quality of livestock and poultry cultivation environment and the quality of improving the livestock and poultry animal product, prevent that also the people from suffering from the possibility of Streptococcus suis simultaneously.Fundamentally reduce and even stop the food origin disease that pathogenic bacteria cause, guarantee that the people's is healthy and safe.
When (2) being used for the elimination of birds, beasts and eggs and meat curing, transportation environment pathogenic bacteria, with fresh egg, duck's egg, pork, pork is example, testing result as depicted in figs. 1 and 2, to salmonella (Salmonellasp), Streptococcus suis (Streptococcus suis), the clearance rate of clostruidium (Clostridium sp) and Escherichia coli (Escherichia coli) all can reach more than 99%.Can prolong the preservation time under normal temperature or low temperature effectively, prevent or delay birds, beasts and eggs and meat is putrid and deteriorated, avoid using chemical preservative simultaneously, convenient, safe and effective, directly improved product quality.
(3) should be specially adapted in birds, beasts and eggs and the meat packing process with method, be the facility that processing provides high-quality raw material and processes, guarantee the high-quality of Poultry and Eggs converted products, solve food-safety problem effectively.
2, adopting the present invention to eliminate birds, beasts and eggs and meat, to carry the pathogenic bacteria security good
Bdellovibrio is eliminated the salmonella that birds, beasts and eggs meat carries, Streptococcus suis, clostruidium and colibacillary method are biological methods, Bdellovibrio have can infect, the characteristic of cracking host bacteria, make it to be suitable as the biological cleaning factor of restraining or removing pathogenic bacteria in organism and the environment thereof, and it can be withered away automatically because of hungry behind the intact host bacteria of cracking, therefore humans and animals is had no side effect.Reduce or eliminate chemicals such as residues of antibiotics effectively, guarantee the high-quality of livestock product, fundamentally avoid the generation of bird product food poisoning.Improve the safety coefficient of edible birds, beasts and eggs of consumer and meat, protected consumer's health.
3, also extensible other animal product and the aquatic products of being applied to of the present invention, be particularly conducive to and reduce or eliminate chemicals such as antibiotic use and residual, guarantee the high-quality of aquatic products and other animal product, to improving edible safety, ensure that human health has crucial meaning; Escort for the export trade simultaneously.
Description of drawings
Fig. 1 is that Bdellovibrio and mixed liquor thereof carry the test effect comparison diagram of four kinds of pathogenic bacteria on elimination egg surface.
Fig. 2 is the test effect comparison diagram of Bdellovibrio and mixed liquor four kinds of pathogenic bacteria that carry on the surface in eliminating the pork process thereof.
The specific embodiment
Embodiment 1
Bdellovibrio is at the application test of eliminating the common pathogen that the new fresh hen egg in birds, beasts and eggs field carries
Test comprises following concrete steps and condition thereof:
Step 1: the separation and purification of Bdellovibrio
Water body or the bed mud of gathering carried out preliminary treatment, the Aeromonas hydrophila of inoculation propagation Bdellovibrio, fall double-layer plate, plaque appears on the double-layer plate that contains the host bacterium, single spot that picking constantly enlarges, cultivate by micro-biological process liquid propagation again, the double-layer plate check, single spot of constantly enlarging of picking once more, and repeated several times, to single plaque formation shape that goes down to posterity, size, the plaque that transparency is all consistent, be the pure bacterial strain of a strain Bdellovibrio, by the big subtotal of single spot in the same time, isolate the pure bacterial strain of 2 strain Bdellovibrios, described Bdellovibrio is being positioned at the Chinese typical culture collection center preservation of Chinese Wuhan City Wuhan University on January 13rd, 2008, Bdellovibrio sp.BDF01, deposit number are CCTCC NO:M 208008 and Bdellovibrio sp.BDF02, and deposit number is CCTCC NO:M 208009.
Step 2: the preparation of Bdellovibrio liquid
The bacterial strain that step 1 obtains number is respectively the Bdellovibrio of BDF01, BDF02, and joins in the erlenmeyer flask that contains the DNB culture medium after Aeromonas hydrophila liquid mixes, and constant temperature shaking table 250rpm, 28 ℃ cultivate 24h.Culture is got supernatant through 6000rpm, 4 ℃ of centrifugal 20min, and centrifugal 20min under 16000rpm, the 4 ℃ of conditions abandons supernatant again, adds an amount of DNB fluid nutrient medium Bdellovibrio sediment that suspends again, and making concentration is 10
2～10
12The Bdellovibrio liquid of pfu/ml all is called the Bdellovibrio mixed liquor with above-mentioned 2 strain bacterium with the arbitrary proportion mixing.
Step 3: eliminate salmonella, Streptococcus suis, clostruidium and colibacillary application experiment
(1) salmonella, Streptococcus suis, clostruidium and colibacillary preparation
Cultivate with common nutrient broth (NB) culture medium shaking table, 160rpm cultivates 12-16h for 28 ℃ and breeds salmonella respectively, Streptococcus suis, clostruidium and Escherichia coli, nutrient solution is through 5000rpm, 4 ℃ of centrifugal 20min, abandon supernatant, the precipitation thalline suspends again with sterilized water, and making its concentration is 10
9Cfu/ml prepares the mixed liquor of above-mentioned four kinds of bacterium at last, and the final concentration of every kind of bacterium is 10
5Cfu/ml.
(2) use BDF01 or BDF02 Bdellovibrio liquid and respectively respectively in BDF01: BDF02 be the Bdellovibrio mixed liquor that mixes of 1: 1,1: 2,2: 1 ratio to salmonella, Streptococcus suis, clostruidium and colibacillary elimination test
The test material therefor is an egg, and contrast and experimental group are all established in test.Three parallel samples are established in contrast, and each parallel sample has 10 in egg; In the experimental group, egg be impregnated in 4 kinds of pathogenic bacteria mixed liquors of step 3 (1), take out after 30 seconds and drain.Then per 10 eggs are divided into a group, impregnated in concentration is 10
2Pfu/ml, 10
4Pfu/ml, 10
6Pfu/ml, 10
8Pfu/ml, 10
10Pfu/ml or 10
12In the Bdellovibrio liquid of pfu ml.It is BDF01 or BDF02 and Bdellovibrio mixed liquor that the Bdellovibrio liquid of variable concentrations is all established individual plant.Egg takes out after 30 seconds and drains behind different Bdellovibrio liquid dipping, at room temperature places 4 hours, detects the content of above-mentioned four kinds of pathogenic bacteria afterwards respectively.Salmonella, Streptococcus suis, clostruidium and colibacillary concrete detection method are to learn from else's experience the moistening sterilization swab (swab) of SPSS after smearing 4 times uniformly on the egg surface, put vibration, mixing in the aseptic centrifuge tube that contains SPSS, adopt MPN (most probable number) method that bacterium is counted (unit is the cfu/ml/ egg) then.Wherein transparent indication selective medium is adopted in the detection of Streptococcus suis, XLD (xylose-lysine-deoxycholate) selective medium is adopted in the detection of salmonella, clostruidium detects adopts the egg yolk agar culture medium, and TBX (Tryptone BileX-glucuronide) selectivity agar medium is adopted in colibacillary detection.
With BDF01 or BDF02 least concentration (10
2The result of the test group of 1: 1 Bdellovibrio mixed liquor of Bdellovibrio liquid pfu/ml) and mixing ratio is an example, describes eliminating effect.Testing result as shown in Figure 1.Fig. 1 shows, in the experimental group of using the Bdellovibrio mixed liquor, when 4 hours off-test, pathogenic bacteria almost detect less than, promptly eliminate fully; And the experimental group of individual plant Bdellovibrio (BDF01 or BDF02) of using same concentration is slow slightly to the elimination of four kinds of pathogenic bacteria, and the quantity that still can detect pathogenic bacteria this moment is the 2-3cfu/ml egg, and it is slightly poorer than the effect of 2 strain Bdellovibrio mixed liquors to eliminate effect.
Relatively initial concentration and the final concentration of four kinds of pathogenic bacteria, individual plant or 2 strain Bdellovibrio mixed liquors are to salmonella as can be known, Streptococcus suis, clostruidium and Escherichia coli elimination factor all can reach more than 99%.Because the effect of other Testing Team is identical or better, so do not repeat them here.
Embodiment 2
Bdellovibrio is at the application test of eliminating the common pathogen that neat of animal carries
Test comprises following concrete steps and condition thereof:
Step 1, two and step 3 (1) all with embodiment 1
Step 3 (2) 2 strain Bdellovibrio Bdellovibrios are to eliminating salmonella, Streptococcus suis, clostruidium and the coli test that carry on the surface in the pork process
Be partitioned into the laboratory sample pig piece of every similar weight, every heavily about 0.25kg, control group and experimental group are established in test, and wherein control group is three, is three parallel samples, and remaining Pork-pieces is an experimental group, and three of every groups are three parallel samples.They be impregnated in the pathogenic bacteria mixed liquor of step 3 (1), and the pathogenic bacteria mixed liquor is meant that salmonella, Streptococcus suis, clostruidium and colibacillary final concentration reach 1 * 10 respectively
5Cfu/ml, taking-up drains after 30 seconds.Then the pork of each group of experimental group is directly evenly sprayed again the Bdellovibrio liquid of the variable concentrations of different amounts.The sprinkling amount of the Bdellovibrio liquid of every group is 15ml/kg, 35ml/kg, 55ml/kg, 75ml/kg or 100ml/kg; The concentration of Bdellovibrio liquid is 10
2Pfu/ml, 10
4Pfu/ml, 10
6Pfu/ml, 10
8Pfu/ml, 10
10Pfu/ml or 10
12Pfu ml.The Bdellovibrio liquid of variable concentrations all mixed by 1: 1,3: 2,2: 3, preparation Bdellovibrio mixed liquor.
Pork-pieces after above-mentioned Bdellovibrio liquid sprays places under the room temperature, and test period is 4 hours, detects the content of pathogenic bacteria afterwards respectively, and the concentration detection method of four kinds of pathogenic bacteria adopts MPN detection method (unit is cfu/kg).Detect used specificity culture medium with embodiment 1.
With BDF01, BDF02 least concentration (10
2The test group of the Bdellovibrio mixed liquor of Bdellovibrio liquid pfu/ml) and mixing ratio 1: 1, sprinkling amount minimum (15ml/kg) is an example, describes eliminating effect.Testing result as shown in Figure 2.As can be seen from Figure 2, in the experimental group of using the Bdellovibrio mixed liquor, when 4 hours off-test, pathogenic bacteria detect less than, promptly eliminate fully; And the experimental group of using the individual plant Bdellovibrio (BDF01 or BDF02) of same concentration is omited slowly the elimination of four kinds of pathogenic bacteria, and the quantity that still can detect pathogenic bacteria this moment is 10cfu/kg, and it is poorer slightly than the effect of 2 strain Bdellovibrio mixed liquors to eliminate effect.
Relatively initial concentration and the final concentration of four kinds of pathogenic bacteria, 2 strain Bdellovibrio mixed liquors are to salmonella as can be known, Streptococcus suis, clostruidium and Escherichia coli elimination factor can reach more than 99%.Because the removing effect of other Testing Team is identical or better, so do not repeat them here.
About evaluation to meat matter, make the detection except above-mentioned safe and sanitary aspect from meat, also comprise from retention ability, pH, tender degree, yellowish pink and hardness and the several aspects of humidity and examining or check.
Retention ability is according to present 48 hours general methods of dripping, meat products is suspended on 0 ℃-4 ℃ suspension storages calculates weight-loss ratio after 48 hours, the pork of the experimental group of use Bdellovibrio is all higher at suspension 48h specific retention, and weight-loss ratio average out to 2.2%, the weight-loss ratio of control group then are 11%.Obviously find out the decline of control group meat quality thus.
Estimate from yellowish pink, use the pork of Bdellovibrio to present cerise, glossy slightly, and do not use the pork yellowish pink of Bdellovibrio greyish white, the gloss dimness, and constantly have water to exosmose.The subjective assessment pork of infection pathogen (control group) yellowish pink as can be seen significantly is inferior to experimental group.
From the pH pH-value determination pH, the pH of the pork of infection pathogen (control group) is near 5, this is the sign of the retention ability decline of muscle, the pH value of this moment produces considerable influence to the local flavor of pork, and use the experimental group pH value of Bdellovibrio to maintain about 6.5, good than control group of the quality maintenance of this explanation meat.
Tender degree is mainly assessed by tender degree device, and the result of assessment does not have difference clearly between the two, the tender degree of experimental group slightly be better than control group.
Subjective assessment hardness mainly whether determine by tangent plane by easy deformation, and estimate humidity mainly is by how much determining that tangent plane liquid oozes out, the pork tangent plane of control group is easy to distortion during 4h, and also has water to ooze out, can rate for oozing out.The pork tangent plane of experimental group is not easy distortion, does not also have water to ooze out, and can rate be moist.
From the comprehensive evaluation of above meat matter, the meat quality of experimental group significantly is better than control group.This explanation Bdellovibrio can be applied to the preservation and the processing industry of birds, beasts and eggs meat in that to eliminate aspect the common pathogen that birds, beasts and eggs meat carries effect remarkable.
Embodiment 3
The application test of the common pathogen of Bdellovibrio in eliminating the pig farm environment
Test comprises following concrete steps and condition thereof:
Step 1 and two is all with embodiment 1
The elimination test of step 3 (1) pathogenic bacteria
With a certain pig farm is example, and one of before not using Bdellovibrio year, because that pig is cultured sanitary condition is bad, the Streptococcus suis in the breeding environment, Escherichia coli etc. are more, often reach 10
4Cfu/cm
2More than.Therefore be their disease of control, plant is everlasting and adds antibiotic in the feed, so causes pork quality to descend, and the export trade is restricted.Simultaneously, the breed sanitary condition is bad, and pork pig is prone to diseases such as enteritis, swine plague.
In order to explore health, green method for culturing pigs, application process of the present invention try out on this pig farm, is divided into a plurality of different tests groups, is 10 to the even on one's body spray concentration of hurdle circle, apparatus, feed, pig of pig
2Any mixed liquor of 2 strain Bdellovibrios of pfu/ml (present embodiment adopted 1: 1) sprays use amount and then is respectively 15ml/m by the difference of test group
2, 35ml/m
2, 55ml/m
2, 75ml/m
2, or 100ml/m
2, sprayed once every 2 days.During environment germ subsequently detects, be 15ml/m only in the sprinkling amount
2Detect a spot of Escherichia coli in the test group, the test group pathogenic bacteria of other sprinkling amounts almost detect less than.So use, over a year, though antibiotic has stopped use, the disease of pig does not have to take place substantially.Every detection when selling as pork pig is all qualified, has reached export standard, and its meat is antibiotic better than having added in feed.
Result of this research on probation shows that Bdellovibrio pathogenic bacteria even effect of preventing and treating aspect its disease in eliminating the livestock and poultry cultivation environment are remarkable, can improve the physique of pig, and medicine or antibiotic use are eliminated in the generation of the disease of control pig, promote meat quality.This shows that the Bdellovibrio microbial inoculum can be applied to the livestock and poultry cultivation base widely.
Embodiment 4
Bdellovibrio is at the application test of the preservation environment of eliminating birds, beasts and eggs and meat, common pathogen that means of transport carries
Test comprises following concrete steps and condition thereof:
Step 1 and two is all with embodiment 1
The elimination test of step 3 (1) pathogenic bacteria
Adopt application process of the present invention, be divided into a plurality of different tests groups, to freezer ground and body of wall, transportation birds, beasts and eggs and the meat container inner surface of preservation birds, beasts and eggs and meat, evenly spray concentration is 10
2The 2 strain Bdellovibrios of pfu/ml are sprayed use amount and then are respectively 10ml/m by the difference of test group by the mixed liquor of mixing in 1: 1
2, 30ml/m
2, 50ml/m
2, 70ml/m
2, or 100ml/m
2, spray once every day, sprayed continuously two days, during the environment germ subsequently detects, is 10ml/m in the sprinkling amount only
2Detect a spot of Streptococcus suis in the test group, the test group of other sprinkling amounts does not detect common pathogens such as salmonella, Streptococcus suis, clostruidium and Escherichia coli.The result of this research on probation shows that Bdellovibrio effect aspect the pathogenic bacteria in eliminating preservation, transportation is remarkable, can guarantee birds, beasts and eggs and meat products quality.This shows that Bdellovibrio can be applied to the preservation and the transportation of birds, beasts and eggs and meat products widely.
Claims (6)
1. a Bdellovibrio is characterized in that in the application of eliminating the common pathogen that birds, beasts and eggs and meat carries:
2 strain Bdellovibrios are carried out fermented and cultured respectively, and making concentration respectively is 10
2～10
12The Bdellovibrio liquid of pfu/ml, stand-by; The Bdellovibrio mixed liquor or the individual plant Bdellovibrio liquid that adopt above-mentioned 2 strain Bdellovibrios to mix by arbitrary proportion, method with sprinkling or obliterating or dipping is handled birds, beasts and eggs and the meat products that carries pathogenic bacteria, and wherein adopt the method for dipping: Bdellovibrio concentration reaches 10 at least
2Pfu/ml; Adopt the method for sprinkling or obliterating: working concentration 〉=10
2During the Bdellovibrio liquid of pfu/ml, directly apply to birds, beasts and eggs and meat products, its use amount reaches 15ml/kg at least; Be applied to environment, its use amount reaches 10ml/m at least
2
Described 2 strain Bdellovibrios are meant the typical culture collection center preservation in China, and Bdellovibrio sp.BDF01, deposit number are CCTCC NO:M 208008 and Bdellovibrio sp.BDF02, and deposit number is CCTCC NO:M 208009.
2. a kind of Bdellovibrio according to claim 1 is in the application of eliminating the common pathogen that birds, beasts and eggs and meat carries, and it is characterized in that: the mixed proportion of described Bdellovibrio mixed liquor is: BDF01: BDF02 is 1: 1.
3. a kind of Bdellovibrio according to claim 1 is characterized in that in the application of eliminating the common pathogen that birds, beasts and eggs and meat carries: described applied environment comprises livestock and poultry farm breeding environment, preservation environment and birds, beasts and eggs and meat products means of transport, process.
According to claim 1 or 3 described a kind of Bdellovibrios in the application of eliminating the common pathogen that birds, beasts and eggs and meat carries, it is characterized in that: when described application process is used for the elimination of livestock and poultry farm breeding environment pathogenic bacteria, adopt the method for spraying: when working concentration 〉=10
2During the Bdellovibrio liquid of pfu/ml, the use amount scope is 35～55ml/m
2
According to claim 1 or 3 described a kind of Bdellovibrios in the application of eliminating the common pathogen that birds, beasts and eggs and meat carries, it is characterized in that: when described application process is used for the elimination of birds, beasts and eggs and meat curing or transportation environment pathogenic bacteria, adopt the method for spraying: when working concentration 〉=10
2During the Bdellovibrio liquid of pfu/ml, the use amount scope is 30～50ml/m
2
6. a kind of Bdellovibrio according to claim 1 is in the application of eliminating the common pathogen that birds, beasts and eggs and meat carries, it is characterized in that: when described application process was used for eliminating the entrained pathogenic bacteria of birds, beasts and eggs and meat packing process, adopt the method for dipping: the Bdellovibrio concentration range was 10
4～10
6Pfu/ml, the method for employing sprinkling or obliterating: working concentration 〉=10
2During the Bdellovibrio liquid of pfu/ml, the use amount scope is 35-55ml/kg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100301588A CN101341901B (en) | 2008-08-13 | 2008-08-13 | Application of bdellovibrio in removing usual pathogen in fowls egg and meat |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100301588A CN101341901B (en) | 2008-08-13 | 2008-08-13 | Application of bdellovibrio in removing usual pathogen in fowls egg and meat |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101341901A CN101341901A (en) | 2009-01-14 |
| CN101341901B true CN101341901B (en) | 2010-09-08 |
Family
ID=40244107
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008100301588A Expired - Fee Related CN101341901B (en) | 2008-08-13 | 2008-08-13 | Application of bdellovibrio in removing usual pathogen in fowls egg and meat |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101341901B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101991029B (en) * | 2010-08-31 | 2012-10-31 | 华南理工大学 | Application of Bdellovibrio mixture to preventing and controlling bacteria in sashimi production process |
| CN101978862B (en) * | 2010-08-31 | 2012-09-26 | 华南理工大学 | Application of bdellovibrio leech plastid in preventing and controlling bacteria in production process of raw steaks |
| CN101978863B (en) * | 2010-08-31 | 2012-12-05 | 华南理工大学 | Application of bdellovibrio sp. telotroch to prevention and control of bacteria in sashimi making process |
-
2008
- 2008-08-13 CN CN2008100301588A patent/CN101341901B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN101341901A (en) | 2009-01-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ferens et al. | Escherichia coli O157: H7: animal reservoir and sources of human infection | |
| CN101356927B (en) | Use of Bdellovibrio in eliminating pathogenicity vibrio in marine products and breeding water body thereof | |
| CN101730478A (en) | Pet food | |
| CN105176874B (en) | Bacillus coagulans FM603 and its application | |
| CN103320365B (en) | Fish-sourced aeromonas hydrophila disease antagonistic strain and application thereof | |
| CN101649299B (en) | Bdellovibrio bacteriovorus bacterial strain for eliminating Listeria monocytogenes and application thereof | |
| CN101341901B (en) | Application of bdellovibrio in removing usual pathogen in fowls egg and meat | |
| Umeh et al. | Antimicrobial resistance profile of Salmonella isolates from livestock | |
| CN114097927A (en) | Preparation method of biological feed additive based on bacteriophage | |
| CN109874920A (en) | A kind of composite microbial feed additive and preparation method thereof | |
| CN106418003B (en) | A kind of aquatic livestock probiotics having both water purification effect | |
| CN108795829B (en) | Bacillus subtilis and application thereof in microbial inoculum and pork pig feed | |
| KR101420836B1 (en) | Livestock feed additive with the culture medium of Latobacillus plantarum | |
| Musa et al. | Prevalence and antibiotic sensitivity pattern of Salmonella isolates from milk products and water reservoirs in maiduguri, north-eastern Nigeria | |
| CN101294142A (en) | Tunica mucosa zymocyte agent and prepraring method thereof | |
| Owoseni et al. | Antimicrobial resistance and virulence genes profiling of proteus species from poultry farms in Lafia, Nigeria | |
| CN108660100B (en) | Bacillus subtilis and application thereof in microbial inoculum and broiler feed | |
| CN111990453A (en) | Lactic acid bacteria-reducing liquid and preparation method and application thereof | |
| Pelic et al. | Health status and microbial quality of common carp reared in a pond fed with treated wastewater from a slaughterhouse | |
| English | Effect of biosecurity and management practices on the prevalence of Salmonella, Campylobacter, and Clostridium perfringens in a poultry production system | |
| Hasan et al. | Prevalence and antibiotic resistance pattern of Escherichia coli isolated from raw dairy milk | |
| CN114292784B (en) | Microbial agent for degrading ammonia and application thereof | |
| Haque et al. | Prevalence of Salmonella in dressed and cooked broiler meat of different grocery shops, hotels and restaurants in Gazipur and Dhaka city of Bangladesh | |
| CN109161501A (en) | A kind of feeding bacillus licheniformis and its application | |
| CN101693880B (en) | Microbial preparation for controlling and eliminating mastitis pathogenic bacterium and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: WANG ZHENGJUN Free format text: FORMER OWNER: SOUTHERN CHINA UNIVERSITY OF TECHNOLOGY Effective date: 20110120 Owner name: WANG HONG |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 510640 NO.381, WUSHAN ROAD, TIANHE DISTRICT, GUANGZHOU CITY, GUANGDONG PROVINCE TO: 100045 NO.1131, NEW BUILDING 11, DONGLI, ER QI JUCHANG ROAD, XICHENG DISTRICT, BEIJING |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20110120 Address after: 100045 Beijing City, Xicheng District 27 theater Ludonglixin 11 floor No. 1131 Co-patentee after: Wang Hong Patentee after: Wang Zhengjun Address before: 510640 Tianhe District, Guangdong, No. five road, No. 381, Patentee before: South China University of Technology |
|
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100908 Termination date: 20130813 |