CN101338293B - 蛭弧菌微生态制剂的生产方法 - Google Patents
蛭弧菌微生态制剂的生产方法 Download PDFInfo
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- CN101338293B CN101338293B CN2008101457095A CN200810145709A CN101338293B CN 101338293 B CN101338293 B CN 101338293B CN 2008101457095 A CN2008101457095 A CN 2008101457095A CN 200810145709 A CN200810145709 A CN 200810145709A CN 101338293 B CN101338293 B CN 101338293B
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- bdellovibrio
- host bacterium
- ecological preparation
- preparation
- bacterium
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- 241000604933 Bdellovibrio Species 0.000 title claims abstract description 57
- 238000002360 preparation method Methods 0.000 title claims abstract description 38
- 238000004519 manufacturing process Methods 0.000 title abstract description 8
- 241000894006 Bacteria Species 0.000 claims abstract description 67
- 238000000034 method Methods 0.000 claims abstract description 19
- 239000000843 powder Substances 0.000 claims abstract description 16
- 238000000855 fermentation Methods 0.000 claims abstract description 7
- 230000004151 fermentation Effects 0.000 claims abstract description 7
- 239000001963 growth medium Substances 0.000 claims abstract description 4
- 241000604931 Bdellovibrio bacteriovorus Species 0.000 claims description 22
- 230000001954 sterilising effect Effects 0.000 claims description 13
- 238000004659 sterilization and disinfection Methods 0.000 claims description 13
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 12
- 238000004108 freeze drying Methods 0.000 claims description 11
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 10
- 230000000968 intestinal effect Effects 0.000 claims description 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 8
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 5
- 239000001110 calcium chloride Substances 0.000 claims description 5
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 5
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 4
- 108010080698 Peptones Proteins 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 4
- 239000002054 inoculum Substances 0.000 claims description 4
- 229910052760 oxygen Inorganic materials 0.000 claims description 4
- 239000001301 oxygen Substances 0.000 claims description 4
- 235000019319 peptone Nutrition 0.000 claims description 4
- 239000008399 tap water Substances 0.000 claims description 4
- 235000020679 tap water Nutrition 0.000 claims description 4
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 3
- 239000000872 buffer Substances 0.000 claims description 3
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 claims description 2
- 229940066779 peptones Drugs 0.000 claims description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 238000004321 preservation Methods 0.000 abstract description 2
- 238000001035 drying Methods 0.000 abstract 2
- 230000035755 proliferation Effects 0.000 abstract 2
- 238000012258 culturing Methods 0.000 abstract 1
- 241000607528 Aeromonas hydrophila Species 0.000 description 8
- 201000010099 disease Diseases 0.000 description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 238000009360 aquaculture Methods 0.000 description 4
- 244000144974 aquaculture Species 0.000 description 4
- 239000007607 bdellovibrio medium Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000000306 component Substances 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000287828 Gallus gallus Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 241001442649 Pinnotheres pisum Species 0.000 description 2
- 241000607142 Salmonella Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000005336 cracking Methods 0.000 description 2
- 230000009849 deactivation Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000003071 parasitic effect Effects 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 208000031295 Animal disease Diseases 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 241000545744 Hirudinea Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000607272 Vibrio parahaemolyticus Species 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229940062173 oxygen 70 % Drugs 0.000 description 1
- 229940063666 oxygen 90 % Drugs 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
检测时间(/d) | 实验组蛭弧菌菌数(cfu/ML) | 实验组嗜水气单胞菌数(cfu/ML) | 对照组嗜水气单胞菌数(cfu/ML) |
0 | 2.3×103 | 6.7×109 | 6.7×109 |
1 | 4.9×105 | 5.2×108 | 7.0×109 |
2 | 5.2×106 | 4.9×107 | 8.2×109 |
3 | 3.2×107 | 7.9×106 | 7.9×109 |
4 | 4.8×107 | 5.2×105 | 7.8×109 |
5 | 8.5×107 | 4.3×104 | 8.3×109 |
Claims (5)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN2008101457095A CN101338293B (zh) | 2008-08-11 | 2008-08-11 | 蛭弧菌微生态制剂的生产方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2008101457095A CN101338293B (zh) | 2008-08-11 | 2008-08-11 | 蛭弧菌微生态制剂的生产方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101338293A CN101338293A (zh) | 2009-01-07 |
CN101338293B true CN101338293B (zh) | 2012-03-21 |
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CN2008101457095A Active CN101338293B (zh) | 2008-08-11 | 2008-08-11 | 蛭弧菌微生态制剂的生产方法 |
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CN (1) | CN101338293B (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101637186B (zh) * | 2009-08-28 | 2013-02-20 | 华南理工大学 | 一种防治农作物细菌性病害的菌剂及其制备方法与应用 |
CN102674563B (zh) * | 2012-06-04 | 2014-04-16 | 广东金海润生物科技有限公司 | 新型环保微生物水质净化混合剂 |
CN111549003B (zh) * | 2020-04-30 | 2022-08-02 | 中国科学院青岛生物能源与过程研究所 | 副溶血弧菌噬菌体、蛭弧菌及其应用 |
-
2008
- 2008-08-11 CN CN2008101457095A patent/CN101338293B/zh active Active
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Publication number | Publication date |
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CN101338293A (zh) | 2009-01-07 |
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Owner name: SHANDONG SINDER TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: CUI ZENGXUE Effective date: 20120724 |
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Free format text: CORRECT: ADDRESS; FROM: 266071 QINGDAO, SHANDONG PROVINCE TO: 262200 WEIFANG, SHANDONG PROVINCE |
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Effective date of registration: 20120724 Address after: 262200 Industrial Park, Shun Road, Zhucheng Development Zone, Shandong Patentee after: Shandong Sinder Technology Co., Ltd. Address before: 266071 Academy of life sciences, room 268, Hongkong East Road, Qingdao, Shandong Patentee before: Cui Zengxue |
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Application publication date: 20090107 Assignee: Beijing Xinde Weite Technology Co.,Ltd. Assignor: Shandong Sinder Technology Co., Ltd. Contract record no.: 2014990000225 Denomination of invention: Method for producing microecological preparation of bdellovibrio bacteriourus Granted publication date: 20120321 License type: Exclusive License Record date: 20140421 |
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