CN101294182A - Method for improving hyaluronic acid volume of production of fermentation production with two-stage oxygen dissolving control technology - Google Patents
Method for improving hyaluronic acid volume of production of fermentation production with two-stage oxygen dissolving control technology Download PDFInfo
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- CN101294182A CN101294182A CNA2008101233997A CN200810123399A CN101294182A CN 101294182 A CN101294182 A CN 101294182A CN A2008101233997 A CNA2008101233997 A CN A2008101233997A CN 200810123399 A CN200810123399 A CN 200810123399A CN 101294182 A CN101294182 A CN 101294182A
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- hyaluronic acid
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- dissolved oxygen
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Abstract
A method for increasing the fermentation production of hyaluronic acid with a two-phase dissolved oxygen control technology belongs to the technology field of applying the oxygen carrier to the optimization of the fermentation process. The dissolved oxygen is controlled above 10% during the first phase (0-8h) of fermentation, and 5-10% during the second phase (8-16h) thereof. By the process controlled by the two phases, the fermentation production of the hyaluronic acid is increased to 6-7g/L, whereas the production by contrast with the embodiment is 5.0g/L. The two-phase dissolved oxygen control technology provided by the method for the production of the hyaluronic acid is simple and highly efficient, and has important value for industrial application, as well as certain significance in guiding and inspiring the fermentation production of other amyloses.
Description
Technical field
A kind of method with two-stage oxygen dissolving control technology raising hyaluronic acid volume of production of fermentation production relates to the technical field of oxygen dissolving control technology in the optimizing fermentation application facet.
Background technology
Hyaluronic acid is a kind of high-molecular weight mucopolysaccharide, and its two kinds of synthetic precursor substances are glucuronic acid and N-acetylglucosamine.Hyaluronic acid has in fields such as medicine, beauty treatment, protective foodss widely to be used.Hyaluronic acid can extract from animal tissues such as cockscomb, also can pass through Production by Microorganism Fermentation.Because the fermentation method cost is low, product purity is high, microbe fermentation method just progressively replaces the tissue extraction method.Hyaluronic research mainly concentrates on Optimizing Conditions of Fermentation to Production by Microorganism Fermentation at present, as Optimum of culture medium, and the optimization of DESIGN OF REACTOR optimization and fermentation pattern.For hyaluronic acid fermentation, the dissolved oxygen important influence, so the control mode of dissolved oxygen is most important to the raising of hyaluronic acid volume of production.Utilize the two-stage oxygen dissolving control mode to improve hyaluronic output, do not see bibliographical information as yet.
Summary of the invention
The purpose of this invention is to provide a kind of method, utilize the present invention can significantly improve hyaluronic output with two-stage oxygen dissolving control strategy raising hyaluronic acid volume of production of fermentation production.
Technical scheme of the present invention: a kind of method that improves hyaluronic acid volume of production of fermentation production with two-stage oxygen dissolving control technology, control dissolved oxygen more than 10% at fermentation fs 0-8h, 8-16h controls dissolved oxygen at 5%-10% in the fermentation subordinate phase, with this two-stage oxygen dissolving CONTROL PROCESS, hyaluronic acid volume of production is significantly improved, its output is brought up to 6-7g/L, and the output of comparative examples is 5.0g/L.
Beneficial effect of the present invention: utilize the present invention can significantly improve hyaluronic acid volume of production.Two-stage oxygen dissolving control zymotechnique provided by the present invention is simple, efficient, and important industrial application value is arranged, and the fermentative Production to other polysaccharide has certain guidance and edifies meaning simultaneously.
Embodiment
Comparative examples: the used bacterial classification of the present invention is streptococcus zooepidemicus Streptococcus zooepidemicusWSH-24, (or being designated as H-24) (Wen Qi, Liu Dengru, Chen Jian, stifled state become " add tensio-active agent and promote streptococcus zooepidemicus high yield hyaluronic acid ", " chemical industry progress " 2006,25 (9), 1089～1094; Chinese patent 200610098195.3 is open), changeing the inclined-plane earlier cultivated 12 hours in 37 ℃ of incubators, carried out shake flask fermentation then 14 hours, 37 ℃ of temperature, rotating speed 200rpm fermented in the 7L fermentor tank 16 hours at last, and fermentation condition is: pH 7.0, mixing speed 200rpm, Ventilation Rate 1.0vvm.Fermention medium is (g/L): sucrose: 70; Yeast powder: 25; MgSO
47H
2O:2.0; Na
2HPO
4: 6.3; K
2SO
4: 7.0.Hyaluronic molecular weight is 1,300,000, and output is 5.0g/L.
Embodiment 1: ferment tank adopts two stage culture methods, the same comparative examples of all the other conditions.More than 10%, control dissolved oxygen 5% at fermentation fs (0-8h) control dissolved oxygen in fermentation subordinate phase (8-16h).With this two-stage oxygen dissolving control strategy fermentative production hyaluronic acid, its output is brought up to 6.0g/L.
Embodiment 2: ferment tank adopts two stage culture methods, the same comparative examples of all the other conditions.More than 10%, control dissolved oxygen 7% at fermentation fs (0-8h) control dissolved oxygen in fermentation subordinate phase (8-16h).With this two-stage oxygen dissolving control strategy fermentative production hyaluronic acid, its output is brought up to 6.3g/L.
Embodiment 3: ferment tank adopts two stage culture methods, the same comparative examples of all the other conditions.More than 10%, control dissolved oxygen 10% at fermentation fs (0-8h) control dissolved oxygen in fermentation subordinate phase (8-16h).With this two-stage oxygen dissolving control strategy fermentative production hyaluronic acid, its output is brought up to 7.0g/L.
Claims (1)
1. method that improves hyaluronic acid volume of production of fermentation production with two-stage oxygen dissolving control technology, it is characterized in that at fermentation fs 0-8h control dissolved oxygen more than 10%, 8-16h controls dissolved oxygen at 5%-10% in the fermentation subordinate phase, with this two-stage oxygen dissolving CONTROL PROCESS, hyaluronic acid volume of production is significantly improved.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101233997A CN101294182A (en) | 2008-05-26 | 2008-05-26 | Method for improving hyaluronic acid volume of production of fermentation production with two-stage oxygen dissolving control technology |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101233997A CN101294182A (en) | 2008-05-26 | 2008-05-26 | Method for improving hyaluronic acid volume of production of fermentation production with two-stage oxygen dissolving control technology |
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| CN101294182A true CN101294182A (en) | 2008-10-29 |
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| CNA2008101233997A Pending CN101294182A (en) | 2008-05-26 | 2008-05-26 | Method for improving hyaluronic acid volume of production of fermentation production with two-stage oxygen dissolving control technology |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102517269A (en) * | 2011-12-15 | 2012-06-27 | 江南大学 | Two-stage dissolved oxygen control strategy-based method for producing chymosin |
| CN112553272A (en) * | 2020-12-24 | 2021-03-26 | 华熙生物科技股份有限公司 | Method for improving hyaluronic acid yield |
| CN114672531A (en) * | 2020-12-24 | 2022-06-28 | 江苏万邦医药科技有限公司 | Method for improving escherichia coli protein expression quantity through stage dissolved oxygen control |
-
2008
- 2008-05-26 CN CNA2008101233997A patent/CN101294182A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102517269A (en) * | 2011-12-15 | 2012-06-27 | 江南大学 | Two-stage dissolved oxygen control strategy-based method for producing chymosin |
| CN102517269B (en) * | 2011-12-15 | 2014-02-19 | 江南大学 | Two-stage dissolved oxygen control strategy-based method for producing chymosin |
| CN112553272A (en) * | 2020-12-24 | 2021-03-26 | 华熙生物科技股份有限公司 | Method for improving hyaluronic acid yield |
| CN114672531A (en) * | 2020-12-24 | 2022-06-28 | 江苏万邦医药科技有限公司 | Method for improving escherichia coli protein expression quantity through stage dissolved oxygen control |
| CN112553272B (en) * | 2020-12-24 | 2022-08-09 | 华熙生物科技股份有限公司 | Method for improving hyaluronic acid yield |
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Open date: 20081029 |