CN101284148A - Preparation method of artificial blood vessel and its application - Google Patents
Preparation method of artificial blood vessel and its application Download PDFInfo
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- CN101284148A CN101284148A CNA2008100382354A CN200810038235A CN101284148A CN 101284148 A CN101284148 A CN 101284148A CN A2008100382354 A CNA2008100382354 A CN A2008100382354A CN 200810038235 A CN200810038235 A CN 200810038235A CN 101284148 A CN101284148 A CN 101284148A
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Abstract
The invention relates to a method for preparing an artificial vessel and the application thereof. The preparation method comprises the following steps: (1) in the preparation of membrane liquid, polymer that is 7 to 30 percent by weight proportion is added into organic solvent the weight proportion of which is 70 to 93 percent, so that casting solution is obtained through swelling and solution under the temperature between 40 DEG C and 80 DEG C; (2) in the preparation of the artificial vessel, a hollow fibre membrane is formed through the filtration and deaeration of the membrane liquid, serosity is squeezed out from a spinneret composed of two concentric tubes, the serosity which enters into precipitating bath through dry spinning then is processed through a post treatment process to obtain the artificial vessel. The method includes the application that the artificial vessel is transplanted to a physical organ or any organs which need quantities of vascular tissues to transport aliment, gas and waste. The artificial vessel obtained by using the preparation method has good adaptability, penetrability and biocompatibility, matches the self vessels, and has controllable outside diameter and wall thickness of the materials.
Description
Technical field
The invention belongs to the preparation and the application of artificial organ, particularly relate to a kind of artificial blood vessel's preparation method and application.
Background technology
The artificial blood vessel who is made by synthetic high polymer who uses clinically mainly contains polyethylene terephthalate (PET), expanded PTFE (ePTFE) and polyurethane (PU) etc. at present.Polyethylene terephthalate mainly is woven into tubulose with the form of polyster fibre, has hole between fiber and can make endothelial cell growth; The artificial blood tube wall that expanded PTFE is made has porous network structure, helps the growth of endotheliocyte.But terylene and expanded PTFE do not have biological activity and anticoagulating active, transplant the back thrombosis easily takes place.Polyurethane has excellent biological compatibility and blood compatibility, and effectively antithrombotic forms.
Patent ZL 03113297.9 (utilizing the artificial blood vessel and the manufacture method thereof of natural torsion synthetic fibers and protein fiber blending) adopts the method for blending to make the artificial blood vessel, polyethylene terephthalate and natural protein fibre combination, given the artificial blood vessel good blood compatibility.Patent 200410018251.9 (a kind of artificial endovascular stent and preparation method thereof) adopts expanded PTFE and nick-eltitanium alloy stent to make artificial endovascular stent with the mould integrated approach, divides preparation that a plurality of steps finish blood vessel and integrated.This patent adopts the method for dry-jet wet-spinning, utilize the high polymer of blood compatibility excellence produce respectively different in the artificial blood vessel of external diameters, different-thickness, random length.
Summary of the invention
Technical problem to be solved by this invention provides a kind of artificial blood vessel's preparation method and application, and method is simple for this, and cost is low, and the products obtained therefrom bore is little, has excellent biological compatibility.
A kind of artificial blood vessel's of the present invention preparation method comprises:
(1) joins preparation liquid: the high polymer of 7～30% weight ratios is joined in the organic solvent of 70～93% weight ratios, under 40～80 ℃ of temperature, make casting solution through swelling and dissolving;
(2) preparation artificial blood vessel: above-mentioned film liquid after filtration, after the deaeration, make hollow-fibre membrane, dosing pump rotating speed 3～25r/min, spinning pressure 0.2～0.7Mpa, the spinning head that serosity is formed from two concentric tubees is extruded, and the dry-spinning through 20～200mm enters coagulating bath, pass through postprocessing working procedures again, obtain the artificial blood vessel.
High polymer is Kynoar PVDF, 0～3% the polyether sulfone PES of weight ratio 15～20% or among 3～5% the polysulfones PS one or more in the described step (1).
Kynoar PVDF, polyether sulfone PES, polysulfones PS Properties of Polymer specification are in the described step (1): the molecular weight of Kynoar is 50,000Da～200,000Da; The molecular weight of polyether sulfone PES is 50,000Da～80,000Da; The molecular weight of polysulfones PS is 50,000Da～100,000Da.
The hollow-fibre membrane of described step (1) is to adopt the dry-jet wet spinning preparation.
Organic solvent is selected from one or more in dimethyl sulfoxide, dimethyl formamide, dimethyl acetylamide, the N-methyl-2-pyridine alkane ketone in the described step (1).
Film-forming process is in the described step (2): dosing pump specification 0.35ml/r, and air section length 150mm, spinning speed 26m/min, spinning head inner chamber filling liquid pressure is 1.0～5.0 * 10
-3MPa.
Coagulating bath is that water or organic solvent content 0.1～20wt%, temperature are 25～60 ℃ solution in the described step (2).
Post processing in the described step (2) is reeled with the speed of 25～55m/min for nascent hollow-fibre membrane stretches through four roads, the washing of three roads, and protects the hole and handle; Protecting the hole processing is to adopt the aqueous solution of the monohydric alcohol of 20wt%～60wt% or polyhydric alcohol to protect the hole to handle 24～48h, and monohydric alcohol or trihydroxylic alcohol use separately or use simultaneously.
Described monohydric alcohol or polyhydric alcohol are methanol, ethanol, propanol, ethylene glycol, 1,2-propylene glycol, glycerol etc.
Artificial blood vessel in the described step (2) has unsymmetric structure, and external diameter is 0.2mm-4mm, and wall thickness is 0.04mm-1.4mm, and its shrinkage factor is 15～30%, and pure water flux is 40～600L/m
2.h (0.1MPa), the bovine serum albumin rejection is 60～90%, proof pressure 〉=0.1MPa.
Described artificial blood vessel's application is that the organ or a large amount of vascular tissue of any needs that are transplanted to health carry in the organ of nutriment, gas and refuse.
The present invention adopts the good high polymer-Kynoar of biocompatibility, polyether sulfone, polysulfones, by the dry-jet wet spinning process one-shot forming, make the artificial blood vessel, by adjusting moulding process, can effectively control artificial blood vessel's interior external diameter, wall thickness, density, aperture, porosity equidimension parameter and microstructure, prepared artificial blood vessel has good compliance, permeability, axial tensile strength, burst strength, can be complementary with native blood vessel.
Beneficial effect
(1) but preparation method one-shot forming artificial blood vessel of the present invention and can effectively control its microstructure by adjusting moulding process;
(2) the gained artificial blood vessel is continuously shaped, length without limits, physicochemical property is stable, various processing, sterilization do not influence its performance;
(3) the gained artificial blood vessel possesses certain intensity and compliance, has excellent biocompatibility and biological stability, and especially You Yi blood compatibility can be complementary with biological blood vessel.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
Prepare burden by following component and composition: PVDF 15%; PES 3%; DMAc 82%.Above-mentioned raw materials is added in the dissolvers, under the control of 40～80 ℃ of temperature, makes casting solution through swelling and dissolving, casting solution after filtration, stand-by after the deaeration.Casting solution is spun into hollow-fibre membrane through concentric circular tubular type spinning head, and film-forming process is: dosing pump specification 0.35ml/r, and revolution speed 4.5r/min, air section length 150mm, inside and outside coagulating bath is a water, spinning speed 26m/min.Can obtain the artificial blood vessel through washing, guarantor hole, cold drying and coiling.Vertical shrinkage factor of this artificial blood vessel is 25%, and water flux is 500L/m
2.h, the rejection of bovine serum albumin is 65%.
Embodiment 2
Prepare burden by following component and composition: PVDF 18%; PS 5%; DMAc 77%.With the method dissolving back spinning that above-mentioned raw materials is pressed embodiment 1, vertical shrinkage factor of this artificial blood vessel is 21%, and water flux is 210L/m
2.h, the rejection of bovine serum albumin is 70%.
Embodiment 3
Prepare burden by following component and composition: PVDF 20%; PES 3%; PS 3%; DMAc 74%.With the method dissolving back spinning that above-mentioned raw materials is pressed embodiment 1, vertical shrinkage factor of this artificial blood vessel is 18%, and water flux is 180L/m
2.h, the rejection of bovine serum albumin is 78%.
Artificial blood vessel to the foregoing description 1,2,3 has carried out Performance Detection, result such as following table.
| The artificial blood vessel | Average pore size (μ m) | Porosity (%) | Burst pressure (Mpa) |
| (embodiment 1) | 1.5 | 85 | 0.9 |
| (embodiment 2) | 1.3 | 78 | 1.0 |
| (embodiment 3) | 1.0 | 70 | 1.2 |
Claims (10)
1. an artificial blood vessel preparation method comprises:
(1) joins preparation liquid: the high polymer of 7～30% weight ratios is joined in the organic solvent of 70～93% weight ratios, under 40～80 ℃ of temperature, make casting solution through swelling and dissolving;
(2) preparation artificial blood vessel: above-mentioned film liquid after filtration, after the deaeration, dosing pump rotating speed 3～25r/min, spinning pressure 0.2～0.7Mpa, the spinning head that serosity is formed from two concentric tubees is extruded, and the dry-spinning through 20～200mm enters coagulating bath, pass through postprocessing working procedures again, obtain the artificial blood vessel.
2. artificial blood vessel's according to claim 1 preparation method is characterized in that: high polymer is Kynoar PVDF, 0～3% the polyether sulfone PES of weight ratio 7～25% or among 3～5% the polysulfones PS one or more in the described step (1).
3. artificial blood vessel's according to claim 1 preparation method is characterized in that: the hollow-fibre membrane of described step (1) is to adopt the dry-jet wet spinning preparation.
4. artificial blood vessel's according to claim 1 preparation method is characterized in that: organic solvent is selected from one or more in dimethyl sulfoxide, dimethyl formamide, dimethyl acetylamide, the N-methyl-2-pyridine alkane ketone in the described step (1).
5. artificial blood vessel's according to claim 1 preparation method, it is characterized in that: film-forming process is in the described step (2): dosing pump specification 0.35ml/r, air section length 150mm, spinning speed 26m/min, spinning head inner chamber filling liquid pressure is 1.0～5.0 * 10
-3MPa.
6. artificial blood vessel's according to claim 1 preparation method is characterized in that: coagulating bath is that water or organic solvent content 0.1～20wt%, temperature are 25～60 ℃ solution in the described step (2).
7. artificial blood vessel's according to claim 1 preparation method is characterized in that: the post processing in the described step (2) is reeled with the speed of 25～55m/min for nascent hollow-fibre membrane stretches through four roads, the washing of three roads, and protects the hole processing; Protecting the hole processing is to adopt the aqueous solution of the monohydric alcohol of 20wt%～60wt% or polyhydric alcohol to protect the hole to handle 24～48h, and monohydric alcohol or trihydroxylic alcohol use individually or simultaneously.
8. artificial blood vessel's according to claim 7 preparation method, it is characterized in that: described monohydric alcohol or polyhydric alcohol are methanol, ethanol, propanol, ethylene glycol, 1,2-propylene glycol or glycerol.
9. artificial blood vessel's according to claim 1 preparation method, it is characterized in that: the artificial blood vessel in the described step (2) has unsymmetric structure, and external diameter is 0.2mm-4mm, and wall thickness is 0.04mm-1.4mm, its shrinkage factor is 15～30%, and pure water flux is 40～600L/m
2.h (0.1MPa), the bovine serum albumin rejection is 60～90%, proof pressure 〉=0.1MPa.
10. artificial blood vessel's according to claim 1 preparation method, it is characterized in that: described artificial blood vessel's application is that the organ or a large amount of vascular tissue of any needs that are transplanted to health carry in the organ of nutriment, gas and refuse.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100382354A CN101284148A (en) | 2008-05-29 | 2008-05-29 | Preparation method of artificial blood vessel and its application |
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|---|---|---|---|
| CNA2008100382354A CN101284148A (en) | 2008-05-29 | 2008-05-29 | Preparation method of artificial blood vessel and its application |
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| CN101284148A true CN101284148A (en) | 2008-10-15 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101884810A (en) * | 2010-07-02 | 2010-11-17 | 西南大学 | Method for preparing small-caliber artificial blood vessel from fish intestines |
| CN102871772A (en) * | 2011-07-13 | 2013-01-16 | 冯淑芹 | Porous degradable blood vessel and preparation method thereof |
| CN104096265A (en) * | 2014-07-01 | 2014-10-15 | 温州医科大学 | Preparation method of three-dimensional contraction model for constructing artificial blood vessel model |
| CN105268331A (en) * | 2014-07-09 | 2016-01-27 | 天津工业大学 | PVDF separating membrane with good blood compatibility and preparation method thereof |
| CN103933609B (en) * | 2014-02-28 | 2016-06-08 | 武汉杨森生物技术有限公司 | One-time formed polyurethane artificial blood vessel and preparation method thereof |
| CN105709323A (en) * | 2008-07-09 | 2016-06-29 | 科拉弗洛有限公司 | Methods, Apparatuses And Systems For Caval Stenting For Venous Drainage |
| CN109790654A (en) * | 2016-10-07 | 2019-05-21 | 东丽株式会社 | Tubular fabric |
| CN114832162A (en) * | 2022-05-19 | 2022-08-02 | 浙江大学 | Preparation method of double-layer small-caliber artificial blood vessel based on compliance matching |
-
2008
- 2008-05-29 CN CNA2008100382354A patent/CN101284148A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105709323A (en) * | 2008-07-09 | 2016-06-29 | 科拉弗洛有限公司 | Methods, Apparatuses And Systems For Caval Stenting For Venous Drainage |
| CN101884810A (en) * | 2010-07-02 | 2010-11-17 | 西南大学 | Method for preparing small-caliber artificial blood vessel from fish intestines |
| CN101884810B (en) * | 2010-07-02 | 2012-12-12 | 西南大学 | Method for preparing small-caliber artificial blood vessel from fish intestines |
| CN102871772A (en) * | 2011-07-13 | 2013-01-16 | 冯淑芹 | Porous degradable blood vessel and preparation method thereof |
| CN103933609B (en) * | 2014-02-28 | 2016-06-08 | 武汉杨森生物技术有限公司 | One-time formed polyurethane artificial blood vessel and preparation method thereof |
| CN104096265A (en) * | 2014-07-01 | 2014-10-15 | 温州医科大学 | Preparation method of three-dimensional contraction model for constructing artificial blood vessel model |
| CN104096265B (en) * | 2014-07-01 | 2016-03-23 | 温州医科大学 | A kind of preparation method of the three-dimensional shrinkage model for building artificial blood vessel's model |
| CN105268331A (en) * | 2014-07-09 | 2016-01-27 | 天津工业大学 | PVDF separating membrane with good blood compatibility and preparation method thereof |
| CN105268331B (en) * | 2014-07-09 | 2018-11-09 | 天津工业大学 | A kind of preferable PVDF seperation films of blood compatibility and preparation method thereof |
| CN109790654A (en) * | 2016-10-07 | 2019-05-21 | 东丽株式会社 | Tubular fabric |
| CN114832162A (en) * | 2022-05-19 | 2022-08-02 | 浙江大学 | Preparation method of double-layer small-caliber artificial blood vessel based on compliance matching |
| CN114832162B (en) * | 2022-05-19 | 2022-12-13 | 浙江大学 | Preparation method of double-layer small-caliber artificial blood vessel based on compliance matching |
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