CN101280279B - Phomopsis capable of producing gallic acid - Google Patents
Phomopsis capable of producing gallic acid Download PDFInfo
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- CN101280279B CN101280279B CN2008100642614A CN200810064261A CN101280279B CN 101280279 B CN101280279 B CN 101280279B CN 2008100642614 A CN2008100642614 A CN 2008100642614A CN 200810064261 A CN200810064261 A CN 200810064261A CN 101280279 B CN101280279 B CN 101280279B
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- gallic acid
- phomopsis
- bacterial strain
- amur maple
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Abstract
The invention relates to gene stock of phomopsis bacteria which can produce gallic acid, which belongs to the technical field of the bio-engineering. The bacterial strain CCTCC No:M208015 provided bythe invention is the bacterial strain which is firstly discovered in the phomopsis and can produce gallic acid through fermentation method, the product of the phomopsis bacteria is gallic acid which is the raw material of various medicinal substances. The gallic acid can be produced through the microorganism method, therefore to protect the environment and the amur maple plant resource, and to provide the possibility of the industrialization production of the gallic acid.
Description
Technical field
One strain can be produced the Phomopsis bacterium (Phomopsis sp.) of gallic acid, the present invention relates to a strain microorganism strains, belongs to technical field of bioengineering.
Background technology
(Gallic Acid GA) cries 3,4 again, 5-trihydroxybenzoic acid, Turkey-galls to gallic acid.Being to separate the natural product that obtains from Amur maple (Acer ginnala Maxim) leaf, is a kind of raw material that medicine, chemical industry etc. are produced that can be used for.In medicine industry, gallic acid and derivative thereof to coronary heart disease, cerebral thrombosis, stomach ulcer, antibiotic, schistosomicide, antiviral hepatitis, senile dementia, antitumor etc. demonstrate many-sided effect; At aspects such as chemical industry, light industry, food, gallic acid all is widely used as raw material.
Gallic acid mainly extracts from the Amur maple leaf, and Amur maple mainly is distributed in mountain region, northeast, the Inner Mongol and mountain region, North China in China, and also there is growth the Yangtze valley, mainly is distributed in mountain areas such as the Xiaoxinanlin Mountains, Wanda Mountain, Zhang Guangcai mountain range in the Heilongjiang Province.Though it is very wide to distribute, quantity is few, be scattered about like the stars, seldom grow into forest and poor growth.Because long-term predation formula picking leaves mode has made the Amur maple genetic resources suffer very big waste and destruction, the quality of raw material and quantity are difficult to be guaranteed.
Commerce is produced by Amur maple blade extraction method with gallic acid at present, this method shortcoming is that leaf is subject to seasonal restrictions, scarcity of resources, every production 1g gallic acid, need the 800-1000kg leaf, pluck several thousand big trees, and threat plant life, market is increasing to the demand of gallic acid now, only Heilongjiang Province every year is with regard to 600 tons of planned production, need about 7000 tons of Amur maple cured leafs, conventional breeding technique is still used in present research, so the raw material of gallic acid ensures the most key factor whether it can successfully move towards market that just becomes.Thereby extensively ward off the production approach, solve gallic acid natural resources shortage problem and become very urgent.
Now main research is concentrated on: synthetic, semi-synthetic, tissue culture and aspects such as fungal studies, cultivation.
Up to now, chemists come synthesizing gallic acid to gallic acid is synthetic by means such as tannin hydrolysis, enzymatic conversion method, but the synthetic cost is too high, there is not practical significance from the angle of producing, gallic acid must be obtained from natural goods as the raw material of multiple product, so natural resource exploitation and biotechnology utilization are in the core status that gallic acid is studied.
Produce gallic acid with microbial process, control easy and simple to handle, easy, cost are low.Be that one of approach of application prospect is arranged most.
Summary of the invention
The object of the present invention is to provide a strain can produce the Phomopsis bacterium of gallic acid, thereby for the microbe fermentation method industrial production of gallic acid creates conditions, nurse tree kind of plant resource in the time of the protection environment.
In order to achieve the above object, the present invention has carried out separation, evaluation and meta-bolites analysis to the Amur maple endogenetic fungus on the basis of analyzing domestic and international medicinal plant endogenetic fungus result of study, filter out the endogenetic fungus----Phomopsis of high yield gallic acid.
The technical solution adopted in the present invention is: getting Amur maple stem section is explant, obtains single culture through cultivation after the surface sterilization, separation, purifying.By classical classification and molecular biology method classification, determine that it belongs to Phomopsis (Phomopsis sp.) then; Fermentation culture was collected mycelia in 7 days, detected metabolite with the HPLC method at last, proved that this bacterial classification contains gallic acid.
District between the rRNA of this microorganism strains transcribes (internal transcribedspacer, ITS), sequence fragment 506bp, sequence is:
ACGCGCCCCA?GGCGCACCCA?GAAACCCTTT?GTGAACTTAT?ACCTTACTGT 50
TGCCTCGGCG?CTAGCTGGTC?CTCCGGGGCC?CCTCCTTCTG?GAGGAGCAGG 100
CACGCCGGCG?GCCAACCCAA?CTCTTGTTTT?TACACTGAAA?CTCTGAGCAC 150
AAAACATAAA?TGAATCAAAA?CTTTCAACAA?CGGATCTCTT?GGTTCTGGCA 200
TCGATGAAGA?ACGCAGCGAA?ATGCGATAAG?TAATGTGAAT?TGCAGAATTC 250
AGTGAATCAT?CGAATCTTTG?AACGCACATT?GCGCCCTCTG?GTATTCCGGA 300
GGGCATGCCT?GTTCGAGCGT?CATTTCAACC?CTCAAGCCTG?GCTTGGTGAT 350
GGGGCACTGC?TTCTTACCCG?AGAAGCAGGC?CCTGAAATTC?AGTGGCGAGC 400
TCGCCAGGAC?CCCGAGCGCA?GTAGTTAAAC?CCTCGCTCTG?GAAGGCCCTG 450
GCGGTGCCCT?GCCGTTAAAC?CCCCAACTTC?TGAAAATTGA?CCTCGGATCA 500
GTAGGA?506
Beneficial effect of the present invention: be the suitability for industrialized production of gallic acid,, provide a new way for protection environment, protective plant resource.
This bacterial strain be from Amur maple, separate for the first time endogenetic fungus, output is considerable, cultivates in the 7 the Heavenly Stems bacterium to reach 22.57mg.g
-1, it can provide possibility for the suitability for industrialized production of gallic acid.
The biological material specimens preservation:
Phomopsis CP0701 (Phomopsis.sp.CP0701), preservation date on January 23rd, 2008, depositary institution's title and abbreviation: CCTCC in the Chinese typical culture collection, the numbering of registering on the books CCTCC No:M208015.
Description of drawings
Fig. 1 gallic acid standard specimen liquid chromatogram
Fig. 2 Phomopsis bacterium CP0701 liquid chromatogram.
Claims (1)
1. the Phomopsis bacterium of a strain isolating product gallic acid from the Amur maple plant, its called after Phomopsis bacterium CP0701 (Phomopsis sp.CP0701) that classifies, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, the numbering of registering on the books CCTCC No:M208015.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100642614A CN101280279B (en) | 2008-04-09 | 2008-04-09 | Phomopsis capable of producing gallic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100642614A CN101280279B (en) | 2008-04-09 | 2008-04-09 | Phomopsis capable of producing gallic acid |
Publications (2)
| Publication Number | Publication Date |
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| CN101280279A CN101280279A (en) | 2008-10-08 |
| CN101280279B true CN101280279B (en) | 2010-06-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2008100642614A Expired - Fee Related CN101280279B (en) | 2008-04-09 | 2008-04-09 | Phomopsis capable of producing gallic acid |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154116B (en) * | 2010-12-22 | 2013-06-12 | 浙江大化生物科技股份有限公司 | Endophytic fungus Phomopsis sp. and use thereof |
| CN102154125B (en) * | 2011-03-31 | 2013-06-12 | 浙江大化生物科技股份有限公司 | Phoma strain and application thereof |
| CN102660466B (en) * | 2012-04-26 | 2013-06-12 | 黑龙江大学 | Endophytic fungi for improving content of main active ingredients of schisandra chinensis through fermentation method |
| CN102952757B (en) * | 2012-10-26 | 2014-05-14 | 云南大学 | Paeonia delavayi endophytic phomopsis mold |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1032936A (en) * | 1988-10-08 | 1989-05-17 | 黑龙江省医药工业研究所 | Prepare gallic acid with acer ginnala leaf |
| CN1115335A (en) * | 1994-07-16 | 1996-01-24 | 孙东江 | Method for prodn. of gallic acid by fermentation process |
| CN1903858A (en) * | 2006-08-03 | 2007-01-31 | 厦门大学 | Antitumour compound desacetyl fungus epoxyethyl ester and its preparation method |
-
2008
- 2008-04-09 CN CN2008100642614A patent/CN101280279B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1032936A (en) * | 1988-10-08 | 1989-05-17 | 黑龙江省医药工业研究所 | Prepare gallic acid with acer ginnala leaf |
| CN1115335A (en) * | 1994-07-16 | 1996-01-24 | 孙东江 | Method for prodn. of gallic acid by fermentation process |
| CN1903858A (en) * | 2006-08-03 | 2007-01-31 | 厦门大学 | Antitumour compound desacetyl fungus epoxyethyl ester and its preparation method |
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| CN101280279A (en) | 2008-10-08 |
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Granted publication date: 20100602 Termination date: 20120409 |