CN101241124A - 一种生物芯片基片及其制备方法 - Google Patents
一种生物芯片基片及其制备方法 Download PDFInfo
- Publication number
- CN101241124A CN101241124A CNA2008100577846A CN200810057784A CN101241124A CN 101241124 A CN101241124 A CN 101241124A CN A2008100577846 A CNA2008100577846 A CN A2008100577846A CN 200810057784 A CN200810057784 A CN 200810057784A CN 101241124 A CN101241124 A CN 101241124A
- Authority
- CN
- China
- Prior art keywords
- substrate
- glucosan
- aldehyde radical
- amination
- slide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 239000000758 substrate Substances 0.000 title claims abstract description 93
- 238000000034 method Methods 0.000 title claims description 40
- 229920001503 Glucan Polymers 0.000 claims abstract description 65
- 238000005576 amination reaction Methods 0.000 claims abstract description 33
- 238000000018 DNA microarray Methods 0.000 claims abstract description 25
- 125000003563 glycoside group Chemical group 0.000 claims abstract description 5
- TWNIBLMWSKIRAT-VFUOTHLCSA-N levoglucosan Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@H]2CO[C@@H]1O2 TWNIBLMWSKIRAT-VFUOTHLCSA-N 0.000 claims description 27
- FFUAGWLWBBFQJT-UHFFFAOYSA-N hexamethyldisilazane Chemical compound C[Si](C)(C)N[Si](C)(C)C FFUAGWLWBBFQJT-UHFFFAOYSA-N 0.000 claims description 20
- KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 claims description 14
- 229920002307 Dextran Polymers 0.000 claims description 13
- -1 polysiloxane Polymers 0.000 claims description 13
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 13
- 230000003647 oxidation Effects 0.000 claims description 8
- 238000007254 oxidation reaction Methods 0.000 claims description 8
- 229920001296 polysiloxane Polymers 0.000 claims description 7
- 230000008021 deposition Effects 0.000 claims description 6
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 3
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 claims 3
- 238000002360 preparation method Methods 0.000 abstract description 28
- 108020004707 nucleic acids Proteins 0.000 abstract description 7
- 102000039446 nucleic acids Human genes 0.000 abstract description 7
- 150000007523 nucleic acids Chemical class 0.000 abstract description 7
- 125000003172 aldehyde group Chemical group 0.000 abstract description 6
- 229920002521 macromolecule Polymers 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract description 2
- 230000036186 satiety Effects 0.000 abstract 1
- 235000019627 satiety Nutrition 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 35
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 21
- 239000011159 matrix material Substances 0.000 description 19
- 229920000642 polymer Polymers 0.000 description 13
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 12
- 150000001299 aldehydes Chemical class 0.000 description 12
- JQWHASGSAFIOCM-UHFFFAOYSA-M sodium periodate Chemical compound [Na+].[O-]I(=O)(=O)=O JQWHASGSAFIOCM-UHFFFAOYSA-M 0.000 description 12
- 238000006243 chemical reaction Methods 0.000 description 10
- 238000004375 physisorption Methods 0.000 description 10
- 239000011521 glass Substances 0.000 description 9
- 238000009396 hybridization Methods 0.000 description 7
- 239000007788 liquid Substances 0.000 description 6
- 239000006210 lotion Substances 0.000 description 6
- 229920003023 plastic Polymers 0.000 description 6
- 239000004033 plastic Substances 0.000 description 6
- 238000002444 silanisation Methods 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 5
- 239000004926 polymethyl methacrylate Substances 0.000 description 5
- FZHAPNGMFPVSLP-UHFFFAOYSA-N silanamine Chemical compound [SiH3]N FZHAPNGMFPVSLP-UHFFFAOYSA-N 0.000 description 5
- WYTZZXDRDKSJID-UHFFFAOYSA-N (3-aminopropyl)triethoxysilane Chemical compound CCO[Si](OCC)(OCC)CCCN WYTZZXDRDKSJID-UHFFFAOYSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 4
- 238000000151 deposition Methods 0.000 description 4
- 238000001035 drying Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 125000003368 amide group Chemical group 0.000 description 3
- 239000012141 concentrate Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 229920000661 poly[dimethylsiloxane-co-(3-aminopropyl)methylsiloxane] Polymers 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000252506 Characiformes Species 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 125000003277 amino group Chemical group 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000007405 data analysis Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 229910000077 silane Inorganic materials 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- OKHIGGWUISQLMG-UHFFFAOYSA-N 3-diethoxysilylpropan-1-amine Chemical compound CCO[SiH](OCC)CCCN OKHIGGWUISQLMG-UHFFFAOYSA-N 0.000 description 1
- AHYFYQKMYMKPKD-UHFFFAOYSA-N 3-ethoxysilylpropan-1-amine Chemical compound CCO[SiH2]CCCN AHYFYQKMYMKPKD-UHFFFAOYSA-N 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- KRVSOGSZCMJSLX-UHFFFAOYSA-L chromic acid Substances O[Cr](O)(=O)=O KRVSOGSZCMJSLX-UHFFFAOYSA-L 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 125000003700 epoxy group Chemical group 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- AWJWCTOOIBYHON-UHFFFAOYSA-N furo[3,4-b]pyrazine-5,7-dione Chemical compound C1=CN=C2C(=O)OC(=O)C2=N1 AWJWCTOOIBYHON-UHFFFAOYSA-N 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 239000000017 hydrogel Substances 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000009832 plasma treatment Methods 0.000 description 1
- 229920002627 poly(phosphazenes) Polymers 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000006884 silylation reaction Methods 0.000 description 1
- 230000026676 system process Effects 0.000 description 1
Images
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
样品名称 | 序列 |
PBH | NH2-5’-(T)15-TCGGATTCGACAACACCCGT |
QC | NH2-5’-(T)15-GTG CAA CTC ACT CGA CTG-3’-HEX |
Arab-HEX | HEX-5’-ACTGGACTTGACGGGTGTTGTCGAATCCGA |
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNA2008100577846A CN101241124A (zh) | 2008-02-18 | 2008-02-18 | 一种生物芯片基片及其制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNA2008100577846A CN101241124A (zh) | 2008-02-18 | 2008-02-18 | 一种生物芯片基片及其制备方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN101241124A true CN101241124A (zh) | 2008-08-13 |
Family
ID=39932815
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNA2008100577846A Pending CN101241124A (zh) | 2008-02-18 | 2008-02-18 | 一种生物芯片基片及其制备方法 |
Country Status (1)
Country | Link |
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CN (1) | CN101241124A (zh) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009135388A1 (en) * | 2008-05-09 | 2009-11-12 | Zhiping Liu | A molecule detecting system |
CN101857900A (zh) * | 2010-05-06 | 2010-10-13 | 东华大学 | 一种双环氧基修饰的生物芯片基片的制备方法 |
CN101633742B (zh) * | 2009-09-01 | 2012-05-23 | 博奥生物有限公司 | 氨基塑料基片及其制备方法与应用 |
CN102520151A (zh) * | 2011-12-09 | 2012-06-27 | 东南大学 | 一种准三维生物芯片的制备方法 |
CN102276863B (zh) * | 2009-09-01 | 2013-03-20 | 博奥生物有限公司 | 一种氨基塑料基片及其制备方法与应用 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1381590A (zh) * | 2001-04-13 | 2002-11-27 | 缪金明 | 单核苷酸多态性(snp)的简便快速的高温杂交芯片检测技术 |
-
2008
- 2008-02-18 CN CNA2008100577846A patent/CN101241124A/zh active Pending
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1381590A (zh) * | 2001-04-13 | 2002-11-27 | 缪金明 | 单核苷酸多态性(snp)的简便快速的高温杂交芯片检测技术 |
Non-Patent Citations (1)
Title |
---|
程京: "《生物芯片:生命信息CPU》", 31 January 2006 * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009135388A1 (en) * | 2008-05-09 | 2009-11-12 | Zhiping Liu | A molecule detecting system |
CN101633742B (zh) * | 2009-09-01 | 2012-05-23 | 博奥生物有限公司 | 氨基塑料基片及其制备方法与应用 |
CN102276863B (zh) * | 2009-09-01 | 2013-03-20 | 博奥生物有限公司 | 一种氨基塑料基片及其制备方法与应用 |
CN101857900A (zh) * | 2010-05-06 | 2010-10-13 | 东华大学 | 一种双环氧基修饰的生物芯片基片的制备方法 |
CN101857900B (zh) * | 2010-05-06 | 2013-01-02 | 东华大学 | 一种双环氧基修饰的生物芯片基片的制备方法 |
CN102520151A (zh) * | 2011-12-09 | 2012-06-27 | 东南大学 | 一种准三维生物芯片的制备方法 |
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C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C53 | Correction of patent of invention or patent application | ||
CB02 | Change of applicant information |
Address after: 102206 Beijing City, Changping District Life Science Park Road No. 18 Applicant after: CAPITALBIO CORPORATION Applicant after: Tsinghua University Address before: 102206 Beijing City, Changping District Life Science Park Road No. 18 Applicant before: Capitalbio Corporation Applicant before: Tsinghua University |
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COR | Change of bibliographic data |
Free format text: CORRECT: APPLICANT; FROM: CAPITALBIO CORPORATION TO: CAPITALBIO CORPORATION CO., LTD. |
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Application publication date: 20080813 |