CN101229252A - Agrimony total flavone extract, preparing method and applications thereof - Google Patents
Agrimony total flavone extract, preparing method and applications thereof Download PDFInfo
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Abstract
The invention discloses an extract of agrimony total flavonoids and an extraction and preparation method thereof; the total amount of ketosteroid compounds accounts for 25 percent to 100 percent of the total extraction matter. The invention takes agrimony as raw material and organic solvent whether containing or not containing water as extraction solvent. The extraction procedure is as following: extracting, leaching with solvent and adsorbing resin column to make purification process or using inorganic acid to regulate the PH value of concentrated solution to be no higher than 3, and then obtaining the extraction matter of agrimony after processing. Experiments prove that the extraction matter of agrimony total flavonoids has functions of antioxidation, anti-myocardial ischemia and reducing blood lipid. The extraction matter of agrimony total flavonoids can be used as food preservative and health product additive; the extraction matter of agrimony total flavonoids, carriers acceptable in pharmacy or excipient are made into various pharmaceutical dosage forms for anti-myocardial ischemia and reducing blood lipid. The invention has the advantages of simple technics and good treatment effect and can be widely used for curing or preventing coronary heart disease, hyperlipoidemia, high blood pressure or preventing cardio-cerebrovascular diseases such as atherosclerosis, etc.
Description
Technical field:
The present invention relates to agrimony total flavone extract, and preparation method thereof and this extract as antioxidant, and the application on the preparation cardiovascular medicament.
Background technology:
Herba Agrimoniae is named Herba silenes fortunei, melon Rhizoma et radix valerianae, old tendon Bovis seu Bubali, wolf wheatgrass again, is the aerial parts of Rosaceae herbaceos perennial Agrimony (Agrimonia pilosa Ledeb.).Be born in the hillside sylvan life, roadside, limes marginis.Main product Zhejiang, Jiangsu, Hubei.Main pharmacodynamics is an astringing to arrest bleeding in Chinese medicine, preventing the attack (or recurrence) of malaria, dysentery relieving, detoxifcation.Be used for hemoptysis, haematemesis, malaria, take off power impairment caused by overstrain, carbuncle.
The Pharmacological action study report of Herba Agrimoniae water extract is more.Modern study shows, the water extract of Herba Agrimoniae has heart tonifying, blood pressure lowering, blood sugar lowering, antibiotic, parasite killing and antitumor action.Wherein to have effective ingredient antibiotic, insecticidal action be agrimophol to the Herba Agrimoniae water extract; The effective ingredient of its antitumor action is an agrimonine.But the effective ingredient and the mechanism of action of effect such as the heart tonifying of Herba Agrimoniae water extract, blood pressure lowering, blood sugar lowering are not clear at present.Document is not found the Herba Agrimoniae water extract as yet as antioxidant at present, and as the report for the treatment of cardiovascular and cerebrovascular diseases medicament.
The Pharmacological action study report of agrimonol extract is less relatively.Wang Decai etc. (Taishan Hospital's journal, 2004,25 (1): 7-8) described hairyvein agrimonia herb and bud ethanol extract and had obvious anti-inflammatory and analgesic effect, but its effective ingredient and mechanism of action are unclear by publication.Document does not find that as yet agrimony total flavone extract is used as antioxidant at present, and as the report for the treatment of cardiovascular and cerebrovascular diseases medicament.
Free radical (free radical) is meant from chemical constitution to contain not group, atom or the molecule of sharing electron.Free radical has the height chemism.For life entity, free radical is the intermediate product of multiple biochemical reaction in the vital movement.Under normal circumstances, the intravital free radical of people is to be among the dynamic equilibrium that produces and remove.Free radical is the effective system of defense of body, then can bring adverse effect to the body vital movement as the free radical that can not keep certain level.But free-radical generating too much or removed slowly, and it can cause the various damages of body molecular level, cellular level and histoorgan level by attacking life macromolecule material and various organelle, and the senescence process of acceleration body also brings out various diseases.Up-to-date studies show that, the accumulation of the product of radical reaction has caused the some diseases in many morbid state, and this has been considered to partly to bring out cell injury by free radical increment in the cell and has caused.These are including, but not limited to a cancer, cardiovascular disease, central nervous system disorder, bone disease, aging, presenile dementia, inflammation, rheumatoid arthritis, autoimmune disease, respiratory distress and emphysema etc.
Therefore, in order to remove the unnecessary free radical in the life entity, the free radical scavenger of widespread usage has non-enzyme free radical scavenger (mainly containing vitamin E, vitamin C, beta-carotene and reduced glutathion etc.) and enzyme free radical scavenger (mainly containing superoxide dismutase, catalase and glutathion peroxidase etc.) at present.Though wherein vitamin E has certain removing free radical activity but the precocious side effect of promotion is arranged; Vitamin C, beta-carotene active relatively poor; Enzyme free radical scavenger then range of application is too narrow.Therefore, people strive to find new and effective free radical scavenger constantly.At present, the synthetic antioxidant (BHT, butyl methoxybenzene etc.) and the Natural antioxidant (as tea polyphenols, sesamol, rice bran extract, Quercetin, germ oil extract, rutin, ginkgetin etc.) have been widely used in food, medicine and the cosmetics of everyday use etc.Yet somebody worker's synthetized oxidation preventive agent has certain toxic and side effects.So the Natural antioxidant has become present antioxidant research and development direction.The Herba Agrimoniae aboundresources, cheap and easy to get, and do not see the purposes of bibliographical information agrimony total flavone extract as yet as antioxidant.
The extractive of general flavone of several plant can be used for the treatment of cardiovascular and cerebrovascular disease and effect for reducing blood fat such as myocardial infarction, angina pectoris, myocardial ischemia, and description: patent ZL 02135573.8, ZL 02116910.1, ZL 99119344.X and CN 1403451 are arranged in following document.But do not see that as yet the bibliographical information agrimony total flavone extract is used for the treatment of cardiovascular and cerebrovascular disease and effect for reducing blood fat such as myocardial infarction, angina pectoris, myocardial ischemia.
Summary of the invention:
The present invention has overcome the deficiencies in the prior art, and providing with the flavone compound is the agrimony total flavone extract of main component.
Another object of the present invention provides the preparation method of agrimony total flavone extract.
The present invention also provides the application of above-mentioned agrimony total flavone extract in preparation free radical scavenger or antioxidant.
The present invention also provides the purposes of above-mentioned agrimony total flavone extract in medicine that treats and/or prevents coronary heart disease, atherosclerosis, hypertension, hyperlipemia and health product preparation.
Agrimony total flavone extract provided by the present invention is meant and adopts any method to extract acquisition from the plant Herba Agrimoniae, with the extract of flavone compound as main component (or main functional component), in this extract, the gross weight sum of flavone compound accounts for 25%～100% of extract gross weight.Described flavone compound is meant that chemical constitution belongs to flavonoid, flavonols, flavanone, flavanone alcohols, anthocyan, flavanol compound, two benzene and adjoins ketone, the sugar examination that the chemical compound of osajin, chalcones, dihydrochalcone-type, aurones class, homoisoflavone class and they and monosaccharide or oligosaccharide form or the derivant of other form.Content ratio between the various flavone compounds in the extract can be consistent with the content ratio in the natural plants, also can be not the same with the content ratio in the natural plants.
The extraction preparation method of agrimony total flavone extract of the present invention, make raw material with the Herba Agrimoniae plant, adopt water, moisture or water-free organic solvent makes to extract solvent, described extraction preparation process comprises: water, moisture or aqueous methanol not, ethanol, acetone, ethyl acetate, the mixture as solvent of one or more arbitrary proportions of n-butyl alcohol or its analog, Herba Agrimoniae plant or its are pulverized in the solvent of 5～30 times of material adding weight, through soaking, ooze rumble, decoct or reflux, extract,, leave standstill, filter, concentrate the recovery organic solvent and get extractum, carry out separation and purification then.
Isolation and purification method is: 1. adopt the mixture as solvent of one or more arbitrary proportions of acetone, ethyl acetate, n-butyl alcohol or its analog to extract, organic facies reclaims solvent, and vacuum drying, spray drying or lyophilization get extractive of general flavone.Perhaps 2. adopt adsorption resin column to carry out purification process, the optional flavonoid developer that adopts detects effluent to determine whether resin adsorbs saturated in the upper prop process, be that 5%～20% ethanol water washs (also can) successively with the volumetric concentration of the water of 1～5 times of resin column volume, 0.5～5 times of resin column volume, 3～10 times of resin column volumes of reuse, volumetric concentration are 20%～80% ethanol water eluting, concentrate, and concentrated solution carried out vacuum drying, spray drying or lyophilization, extractive of general flavone.Perhaps 3. with mineral acid with the concentrated solution pH value transfer to 3 or below, room temperature or stand at low temperature, precipitation separation and dry extractive of general flavone.
The method of above-mentioned separation and purification can use separately agrimony total flavone extract, but also combination in any use agrimony total flavone extract.
The weight sum of flavone compound is 25%～100% of an extract gross weight in the agrimony total flavone extract of said method gained.These extractive of general flavone can further adopt conventional plant method purification, various column chromatographies etc. for example, and these methods are self-explantory for those skilled in that art.Therefore, extractive of general flavone of the present invention has defined flavonoid content, no matter the content of each monomeric compound is how, so long as from the plant Herba Agrimoniae, extract and the flavone compound total content in the above-mentioned range of definition, then this extract is within the scope of the invention.
In the extraction process of agrimony total flavone extract of the present invention, adopt the separation means of adsorption resin method, can remove a large amount of impurity, thereby make the effective ingredient enrichment, finished remove impurity and concentrated two procedures simultaneously as the Herba Agrimoniae flavone.The operation of this method is few, can shorten the production cycle, and can remove heavy metal pollution.
The present invention finds that agrimony total flavone extract has free radical scavenging effect and antioxidation.Therefore, agrimony total flavone extract provided by the invention is used for the treatment of or suppresses the medicine that free radical induces an illness.
The present invention finds first that agrimony total flavone extract has and resists myocardial ischemia and effect for reducing blood fat.Therefore, agrimony total flavone extract provided by the invention is used for the treatment of and/or prevents some cardiovascular disease, and as coronary heart disease, it comprises diseases such as angina pectoris, coronary atherosclerosis, myocardial ischemia; Hypercholesterolemia blood fat disease comprises former or concurrent in the hypercholesterolemia blood fat disease of other disease; Cerebrovascular is as cerebral ischemia and/or anoxia; And other associated conditions.
Agrimony total flavone extract of the present invention is used for food preservative, and is fresh-keeping as the animal and plant oils and fats, preservation of fishery, and really, vegetables are fresh-keeping, all kinds of instant food are fresh-keeping etc., also are used for products and health products additive agent.The effect of extract antioxidation is obvious, and extraction process is simple, cost is low, the extraction ratio height, and can make full use of cheap Herba Agrimoniae is raw material.
Agrimony total flavone extract provided by the invention can be itself and pharmaceutically available carrier and/or diluent and adjuvant, is tablet, sugar coated tablet, pill, capsule, injection, suppository, unguentum, the powder formulation that can be inhaled into use, suspension, cream and ointment.
Compared with prior art, the present invention has following tangible advantage: 1, the inventor finds that first flavone compound contained in the medicinal plants Herba Agrimoniae is the chemical compound monoid with important cardiovascular pharmacological activity and antioxidant activity, the inventor adopts suitable extracting method to obtain to have the agrimony total flavone extract of significant cardiovascular and cerebrovascular vessel protective effect, being expected on the one hand provides a kind of new treatment to select to cardiovascular and cerebrovascular disease patient and the free radical patient that induces an illness, for effectively utilizing the Herba Agrimoniae plant resources more, the application of widening the Herba Agrimoniae plant is also significant on the other hand; 2, extracting method operation provided by the invention is few, easy and simple to handle, with short production cycle, and can remove poisonous metal or the metalloid element that pollutes in the medical material; 3, agrimony total flavone extract provided by the invention can be used in and treats and/or prevents some cardiovascular disease, and as coronary heart disease, it comprises diseases such as angina pectoris, coronary atherosclerosis, myocardial ischemia; Hypercholesterolemia blood fat disease comprises former or concurrent in the hypercholesterolemia blood fat disease of other disease; Cerebrovascular is as cerebral ischemia and/or anoxia; Hypertension; And other associated conditions.4, the plant resources wide material sources of agrimony total flavone extract provided by the invention cheaply are easy to get.
The specific embodiment:
Following specific embodiment is that the present invention is carried out more detailed description, but scope of the present invention is not constituted any restriction.
The extraction and the purification of embodiment 1 agrimony total flavone extract
Add Herba Agrimoniae herb 10kg in extraction pot, water 80L boils and extracted 2 hours, emits extracting solution from the extraction pot bottom, adds 50L water again and extracts under the same conditions 1.5 hours.Filter and merge extracted twice liquid.With extracting solution concentrating under reduced pressure and the dry extractum 2kg that gets.
Add Herba Agrimoniae herb 10kg in extraction pot, 60% ethanol 70L boils and extracted 2 hours, emits extracting solution from the extraction pot bottom, adds 60% ethanol 50L again and extracts under the same conditions 1.5 hours.Filter and merge extracted twice liquid.With extracting solution concentrating under reduced pressure and the dry extractum 1.7kg that gets.
Extractum 50g is dispersed in the 0.5L water, uses the 0.5L n-butanol extraction, repeat 3 times, combining extraction liquid reclaim under reduced pressure n-butyl alcohol, vacuum drying get 5g agrimony total flavone extract A.
The above-mentioned agrimony total flavone extract A of 5g is dispersed in the 0.1L water, with AB-8 macroporous adsorptive resins on the speed of 2 times of column volumes per hour, after treating that resin fully adsorbs flavone, successively with 20% ethanol water eluting of the distilled water of 4 times of column volumes and 2 times of column volumes with flush away major part impurity, use 80% ethanol water eluting at last, concentrate, and concentrated solution is carried out lyophilization, get 3g agrimony total flavone extract B.
The content detection of embodiment 2 agrimony total flavone extracts
The making of standard curve: accurately take by weighing rutin standard substance 10.0000mg and be dissolved in 50% ethanol water, be settled to 25mL, get the rutin standard solution of 400 μ g/mL.Accurately measure above-mentioned rutin standard solution 0.0,0.5,1.0,1.5,2.0,2.5mL inserts in the 10mL volumetric flask, adds 50% ethanol water and supplies 5mL, successively adds 5% NaNO
2With 10% Al (NO
3)
3Each 0.3 mL, mixing adds 4% NaOH solution 4mL after placing 5min, uses 50% ethanol water standardize solution at last, and mixing is measured absorbance A in 510nm after placing 10min.Get standard curve regression equation: y=0.9756x+0.0018, R
2=0.9995
Measure: accurately take by weighing each 6.0000mg of agrimony total flavone extract A and B and be dissolved in 50% ethanol water, be settled to 10mL, get the liquid to be measured of 600 μ g/mL.
Accurately measure above-mentioned liquid 1mL to be measured and insert in the 10mL volumetric flask, by the step in the standard curve making measure absorbance A.The flavones content that gets agrimony total flavone extract A and B according to above-mentioned standard curve regression equation calculation is respectively: 60.5% and 85.1%.
The antioxidant activity experiment of embodiment 3 agrimony total flavone extracts
(1) effect of external removing DPPH free radical
DPPH is mixed with the solution of 0.65mM with dehydrated alcohol, faces the time spent dilution.The concentration of DPPH solution in 0.01mM～0.08mM scope with its absorbance linear correlation at the 517nm place, its equation of linear regression is: A=0.1337c (DPPH)-0.0091, R
2=0.9981.
Get the sample solution of 0.1mL variable concentrations, add the DPPH solution mixing of 1.9mL 0.065mM.Under room temperature, react certain hour, under 517nm, measure absorbance A
iReplace sample as blank with coordinative solvent, absorbance is A
Max, the respective sample solution absorbency is A
0Each sample is made parallel sample 3 times, gets its meansigma methods.The removing activity of DPPH free radical is calculated as follows:
Half is removed concentration (EC
50) calculate according to experimental result, the results are shown in Table 1.
(2) the removing activity of superoxide anion
Adopt riboflavin-light-nitroblue tetrazolium system to measure.
Blank light absorption value (A
Blank): get the phosphate buffer of 0.5mL pH 7.5, add 0.3mL 5 * 10
-5Mol/L riboflavin, 0.25mL 0.02mol/L methionine, 5.1 * 10
-4Mol/L nitroblue tetrazolium and 1.0mL sample solvent add deionized water again and are settled to 5.0mL, and 40W sight light irradiation 20min is in 560nm place colorimetric.
Sample light absorption value (A
Sample): get the phosphate buffer of 0.5mL pH 7.5, add 0.3mL 5 * 10
-5Mol/L riboflavin, 0.25mL 0.02mol/L methionine, 5.1 * 10
-4Mol/L nitroblue tetrazolium and 1.0mL sample solution add deionized water again and are settled to 5.0mL, and 40W daylight lamp irradiation 20min is in 560nm place colorimetric.
Ultra-oxygen anion free radical clearance rate=(A
Blank-A
Sample)/A
Blank* 100%
Half is removed concentration (EC
50) calculate according to experimental result, the results are shown in Table 1.
(3) hydroxy radical is removed ability
The generation of hydroxy radical and detection: in a series of 50 mL color-comparison tubes, add 1.5 mL crystal violet (0.4mmolL respectively
-1), 2.0mL FeSO
4Solution (1mmolL
-1), 1.0mL H
2O
2Solution (2.0mmolL
-1).Adjust pH to 4.0 is diluted to 50mL and shakes up, place 30min after, survey the absorbance A at 580nm place
b, mensuration does not add H simultaneously
2O
2The time 580nm place absorbance A
0Then the generation of hydroxy radical can be used Δ A=A
0-A
bCharacterize.
The mensuration of apparent anti-hydroxy radical clearance rate: in last system, add H
2O
2Add a certain amount of antioxidation reagent before, measure its absorbance A
s
Hydroxy radical clearance rate=(A
s-A
b)/(A
0-A
b) * 100%
Half is removed concentration (EC
50) calculate according to experimental result, the results are shown in Table 1.
(4) Total antioxidant capacity
Adopt beta-carotene-linoleic acid system
The reactant liquor preparation: add beta-carotene chloroformic solution, 20 μ L linoleic acids, the 200 μ L polysorbas20s of 2mL 0.2mg/mL in the round-bottomed flask of 100mL, add the distilled water of 100mL immediately after 40 ℃ of following reduction vaporizations are removed chloroform, mixing gets reactant liquor.Need matching while using.
Add 4.8mL reactant liquor and 200 μ L sample solutions in test tube, the adding sample solvent that does not add sample is done contrast, measures its absorbance at the 470nm place behind the mixing, is designated as A
s 0And A
c 050 ℃ of water-bath 2h measure its absorbance again at the 470nm place then, are designated as A
s tAnd A
c t
Suppress beta-carotene oxygenation efficiency=1-(A
s 0-A
s t/ A
c 0-A
c t) * 100%
Half-inhibition concentration (IC
50) calculate according to experimental result, the results are shown in Table 1.
The oxidation resistance of table 1 agrimony total flavone extract
| Remove the EC of DPPH free radical 50 | Remove the EC of ultra-oxygen anion free radical 50 | Remove the EC of hydroxy radical 50 | The IC of Total antioxidant capacity 50 | |
| Get agrimony total flavone extract A | 7.3±0.3μg/mL | 8.5±0.4μg/mL | 81.6±7.2μg/mL | 13.1±0.8μg/mL |
| Get agrimony total flavone extract B | 4.5±0.5μg/mL | 4.9±0.2μg/m | 63.2±8.3μg/mL | 9.8±0.6μg/mL |
The pharmacodynamics of embodiment 4 agrimony total flavone extracts detects
1, the experiment that resists myocardial ischemia of agrimony total flavone extract
Experimental technique: the SD rat, gastric infusion (embodiment 1 extract 10mg/kg, administration 3 days, every day 2 times) after the anesthesia on the 4th, is opened breast knot and is pricked coronary artery, and it is dirty to core after 2 hours, dyeing, the size of observing the myocardial infarction zone.
Experimental result: compare with the blank group, extract administration treated animal myocardial infarction region area behind coronary ligation significantly reduces.
Table 2 is respectively organized myocardial infarction region area ratio after the rat coronary artery ligation
| Group | The infarcted region area is than (%) |
| The blank group | 36.32±1.35 |
| Extract A (10mg/kg) | 21.13±3.52 ** |
| Extract B (10mg/kg) | 19.65±3.89 ** |
Compare with the blank group:
*P＜0.05,
*P＜0.01 (t-test)
2, agrimony total flavone extract blood lipid regulation effect experiment
Experimental technique: rat feeds 1 week of normal diet under experimental situation, and the eye socket blood sampling is surveyed blood fat as normal value.The normal control group is fed normal diet, and the hyperlipidemia model matched group is fed high lipid food, and experimental group is fed high lipid food administration (embodiment 1 extract 5mg/kg) simultaneously, irritates stomach, plucks eyeball after 2 weeks and gets blood, measures every index.Experimental result: the extract administration can significantly reduce serum total cholesterol and low density lipoprotein, LDL content.
Table 3 rat high blood lipid model is respectively organized blood fat reducing experimental result (n=10)
| Group | T-CHOL (mmol/L) | Triglyceride (mmol/L) | Low density lipoprotein, LDL (mmol/L) |
| The normal control group | 2.87±0.33 | 0.93±0.31 | 1.02±0.29 |
| The hyperlipidemia model matched group | 3.24±0.23 | 1.34±0.35 | 1.58±0.32 |
| Extract A (5mg/kg) | 2.28±0.27 ** | 1.30±0.46 | 1.16±0.26 ** |
| Extract B (5mg/kg) | 2.12±0.21 ** | 1.21±0.39 | 1.05±0.28 ** |
Compare with the hyperlipidemia model matched group:
*P＜0.05,
*P＜0.01 (t-test)
Claims (9)
1. an agrimony total flavone extract is characterized in that the total amount of extracting the flavone compound that obtains from the Herba Agrimoniae plant accounts for 25%～100% of extract gross weight.Described flavone compound is meant that chemical constitution belongs to flavonoid, flavonols, flavanone, flavanone alcohols, anthocyan, flavanol compound, two benzene and adjoins ketone, the sugar examination that the chemical compound of osajin, chalcones, dihydrochalcone-type, aurones class, homoisoflavone class and they and monosaccharide or oligosaccharide form or the derivant of other form.
2. the preparation method of agrimony total flavone extract according to claim 1, it is characterized in that may further comprise the steps: water, the moisture or mixture as solvent of one or more arbitrary proportions of aqueous methanol, ethanol, acetone, ethyl acetate, n-butyl alcohol or its analog not, Herba Agrimoniae plant or its are pulverized in the solvent of 5～30 times of material adding weight, through soaking, ooze rumble, decocting or reflux, extract,, leave standstill, filter, concentrate the recovery organic solvent and get extractum, carry out separation and purification then.
3. the preparation method of agrimony total flavone extract according to claim 2, it is characterized in that isolation and purification method is to adopt the mixture as solvent of one or more arbitrary proportions of acetone, ethyl acetate, n-butyl alcohol or its analog to extract, organic facies reclaims solvent, and vacuum drying, spray drying or lyophilization get extractive of general flavone.
4. the preparation method of agrimony total flavone extract according to claim 2, it is characterized in that isolation and purification method is to adopt adsorbent resin to carry out column chromatography, the optional flavonoid developer that adopts detects effluent to determine whether resin adsorbs saturated in the upper prop process, successively with the water of 1～5 times of resin column volume, 0.5 the volumetric concentration of～5 times of resin column volumes is 5%～20% ethanol water washing, the volumetric concentration of 3～10 times of resin column volumes of reuse is 20%～80% ethanol water eluting, concentrate, and concentrated solution carried out vacuum drying, spray drying or lyophilization get extractive of general flavone.
5. the preparation method of agrimony total flavone extract according to claim 2, it is characterized in that isolation and purification method be with mineral acid with the concentrated solution pH value transfer to 3 or below, room temperature or stand at low temperature, precipitation separation and dry extractive of general flavone.
6. the preparation method of agrimony total flavone extract according to claim 2, it is characterized in that isolation and purification method be claim 3,4,5 described isolation and purification method combination in any use agrimony total flavone extract.
7. the purposes of agrimony total flavone extract according to claim 1 is medicine, health product and the food additive that is used to prepare as free radical scavenger or antioxidant.
8. the purposes of agrimony total flavone extract according to claim 1 is to be used to prepare medicine and the health product that treat and/or prevent cardiovascular and cerebrovascular diseases such as coronary heart disease, atherosclerosis, hyperlipemia.
9. according to the medicine or the health product of claim 1,7,8 described agrimony total flavone extracts, it is characterized in that: comprise agrimony total flavone extract and pharmaceutically available carrier and/or diluent and adjuvant, be tablet, sugar coated tablet, pill, capsule, injection, suppository, unguentum, the powder formulation that can be inhaled into use, suspension, cream and ointment.
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| CN101775028A (en) * | 2009-12-28 | 2010-07-14 | 大兴安岭林格贝有机食品有限责任公司 | Method for gathering and purifying agrimonine in agrimony |
| CN102329206A (en) * | 2011-07-26 | 2012-01-25 | 苏州宝泽堂医药科技有限公司 | Method for preparing agrimophol |
| CN103202897A (en) * | 2012-12-12 | 2013-07-17 | 重庆大学 | Agrimonia eupatoria active component for treating and improving insulin resistance, and application and extraction method thereof |
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| CN107669915A (en) * | 2017-11-25 | 2018-02-09 | 衡阳县华盛达农林科技有限公司 | Treat sophora bud oral liquid of cardiovascular and cerebrovascular diseases and preparation method thereof |
| CN108627487A (en) * | 2018-05-15 | 2018-10-09 | 华中科技大学鄂州工业技术研究院 | A kind of method of fluorescent screening adriamycin nanoparticle pharmaceutical injury of lungs active material |
| WO2022086019A1 (en) * | 2020-10-19 | 2022-04-28 | Dongguk University Industry-Academic Cooperation Foundation | A purified extract isolated from agrimonia coreana nakai containing abundant amount of active ingredient, the preparation thereof, the composition comprising the same as an active ingredient for preventing or treating inflammation, allergy and atopic dermatitis and the use thereof |
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| EP4140318A1 (en) * | 2021-08-26 | 2023-03-01 | PM-International AG | Normalization of lipid metabolism |
| CN113907346A (en) * | 2021-09-02 | 2022-01-11 | 广州欣盛通健康管理科技有限公司 | Food with functions of losing weight, reducing blood fat and controlling blood sugar |
| CN114209742A (en) * | 2021-12-27 | 2022-03-22 | 图方便（苏州）环保科技有限公司 | Clostridium perfringens inhibitor and application thereof |
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