CN101215521A

CN101215521A - Four sides separation culture device for multi-bacterial sample

Info

Publication number: CN101215521A
Application number: CN 200810046709
Authority: CN
Inventors: 姜世民
Original assignee: Individual
Current assignee: Individual
Priority date: 2008-01-17
Filing date: 2008-01-17
Publication date: 2008-07-09

Abstract

The invention relates to a pretreatment of multiple bacterial biological samples and a four-side isolated culture device, which comprises a gathering pipe chamber with a sampling pipe and two isolated culture chambers. The characters are that the bottom portion of the gathering pipe chamber is connected with a sample pool of the bottom portion of one of the isolated culture chambers via a channel, two sample pools of the bottom portions of the two isolated culture chambers are connected via an interface channel. The invention reduces complex operating procedure for sampling, shortens isolated culture time, avoids infection through contact between operators and sample copy, thereby ensuring safety of operators, satisfying requirement of that one sample copy is inoculated on four solid mediums and being normalized isolated culture process.

Description

Novel four sides separation culture device for multi-bacterial sample

Technical field

The present invention relates to a kind ofly can provide four kinds of substratum to carry out the device of separation and Culture to a sample, specifically a kind of novel four sides separation culture device for multi-bacterial sample.

Background technology

In Microbiological Lab, often need ight soil, urine, sputum, body fluid and various swab equal samples to carry out separation and Culture to patient.Now general isolation cultivation method be manual streak inoculation to culture dish, put into incubator then and carry out separation and Culture.There is sizable drawback in this operation.Because the pre-treatment of sample is often ignored in manual operations easily, and operator very easily contact with sample in this case, cause infection easily, and health of operators is constituted a serious threat.What is more important, manual operations can't standard streak inoculation processes, and separation and Culture can't be standardized and standardized management.Therefore it can influence the separation and Culture quality of sample.Have applied for that the patent No. is 200520096773.0, denomination of invention is the utility model patent of biological specimen automatization separation and Culture device in applicant and on June 16th, 2005, this patent is made up of two collection tube chamber and separation and Culture chambers by the mutual UNICOM of conduit, and wherein the collection tube chamber is used to place collection tube; The indoor culture plate that the two sides solid medium is housed of separation and Culture also is equipped with the streak inoculation device in the culturing room, streak inoculation device end is connected with transfering loop, and transfering loop carries out automated operation near culture plate by corresponding instrument.Can carry out necessary early stage to the sample in the collection tube during instrument operation handles, treat that pre-treatment finishes back instrument mechanical manipulator and exerted pressure in the top of collection tube, wherein sample liquid successfully flow into the sample pool of bottom, separation and Culture chamber after collection tube bottom was punctured, make transfering loop obtain sample, the driving handle that mechanical manipulator is picked up inoculator again carries out separation and Culture with the sample streak inoculation to the solid medium of culture plate both sides.The shortcoming that the culture apparatus of this structure exists is: for same sample, the indoor culture plate of separation and Culture can only provide the configuration of two kinds of substratum, can not satisfy Microbiological Lab's needs to same sample three or four kind of substratum on carry out streak inoculation needs.

Summary of the invention

Technical problem to be solved by this invention is the defective that exists at currently available products, provides a kind of novel four sides separation culture device for multi-bacterial sample, to remedy the deficiency of currently available products.

Technical scheme of the present invention is achieved in that it comprises collection tube chamber and two separation and Culture chambers that the sample collection pipe is housed, it is characterized in that: the bottom of collection tube chamber is connected by the sample pool of passage with one of them bottom, separation and Culture chamber, and the sample pool of bottom, two separation and Culture chambers is connected by connecting passage.

The present invention's technical scheme preferably is in the described sample collection pipe little magnetic patch to be housed, and little magnetic patch is as the magnetic whipping appts, and the little magnetic patch of this magnetic stirs in the instrument of alternating magnetic field is arranged, and sample and liquid substance thorough mixing carry out sample and handle in earlier stage;

Two indoor rectangle culture plates that are equipped with respectively of separation and Culture are filled with solid medium on the culture plate, the indoor streak inoculation device that also is provided with of two separation and Culture, and streak inoculation device end is connected with transfering loop, and transfering loop is near culture plate.

Described separation and Culture chamber also comprises the valve gap of upper end, the top engagement of valve gap and separation and Culture chamber.

Described inoculator comprises two arms that become forked, and two arms pass valve gap, and the upper end connects two arms by crossbeam, and the end of two arms is connected with transfering loop respectively, and the middle part of crossbeam is connected with control lever.

Described valve gap is a capping, and the feather edge of capping is connected with upper end, separation and Culture chamber, is connected with rectangular culture plate in the middle of the bottom of capping, and the culture plate dress is inserted into the inside of separation and Culture chamber.

It is indoor that the sample collection pipe is put into collection tube, and the bottom of collection tube chamber is an apsacline inclined-plane, and lower near connecting passage mouth place, the place is higher away from the connecting passage mouth.Sample flows in the sample pool of two culturing room bottoms by connecting passage.

Compare with device with traditional method, the present invention has following characteristics:

1) laboratory technician does not contact with sample, has avoided by the infectious danger of sample, has guaranteed that the laboratory technician's is healthy.

2) save numerous and diverse manual program, avoided the personal errors of separation and Culture.

3) can provide four kinds of substratum to carry out separation and Culture to same sample, meet the needs of Microbiological Lab more special biological specimen (as sputum sample) separation and Culture.

4) can obtain good separation and Culture effect.

5) traditional separation and Culture process is standardized and stdn more.

Description of drawings

Fig. 1 is a structural representation of the present invention

Fig. 2 is a sample collection tubular construction synoptic diagram of the present invention

Embodiment

As shown in Figure 1, the present invention includes collection tube chamber 1 that sample collection pipe 4 is housed and two separation and Culture chambers 2.1 and 2.2, the bottom of collection tube chamber 1 is connected with the sample pool of one of them 2.1 bottom, separation and Culture chamber by passage 3, and the sample pool of bottom, two separation and Culture chambers is connected by connecting passage 7.

Little magnetic patch 14 and spatula 6 are housed in the described sample collection pipe, and little magnetic patch 14 is as the magnetic whipping appts, and the little magnetic patch of this magnetic stirs in the instrument of alternating magnetic field is arranged, and sample and liquid substance thorough mixing carry out sample and handle in earlier stage; Two indoor rectangle culture plates 5 that are equipped with respectively of separation and Culture are filled with solid medium on the culture plate 5, also are provided with the streak inoculation device in the two separation and Culture chambers 2.1 and 2.2, and streak inoculation device end is connected with transfering loop 13, and transfering loop 13 is near culture plate 5.Described separation and Culture chamber also comprises the valve gap 9 of upper end, and valve gap 9 meshes with the top of separation and Culture chamber.Described inoculator comprises that two become arm 10 and 11, two arms of forked to pass valve gap 9, and the upper end connects two arms by crossbeam 12, and the end of two arms is connected with transfering loop 13 respectively, and the middle part of crossbeam 12 is connected with control lever 8.Described valve gap is a capping, and the feather edge of capping is connected with upper end, separation and Culture chamber, is connected with rectangular culture plate in the middle of the bottom of capping, and the culture plate dress is inserted into the inside of separation and Culture chamber.Sample collection pipe 4 is put into collection tube chamber 1, and the bottom of collection tube chamber is an apsacline inclined-plane, and it is lower to enter the mouth out near connecting passage, higher away from the connecting passage ingress.Sample flows in the sample pool of two culturing room bottoms by connecting passage.

Collect such as ight soil, urine, sputum or various swab sample with sample collection pipe 4 during use, putting into separation and Culture device collection tube places indoor, the separation and Culture device that will have a sample collection pipe is placed on the automatic separation and Culture instrument in the sample disk, the alternating magnetic field that instrument provides orders about the interior little magnetic patch 14 of sample collection pipe and stirs in certain space, make thorough mixing such as sample and enrichment liquid, liquid transfer matrix, Digestive system, to carry out the pre-treatment of sample.The time of pre-treatment can manually be set according to the difference of specimen types.After pre-treatment finished, the mechanical manipulator of instrument can be exerted pressure to the top of collection tube, caused the collection tube bottom damaged, sample flows out from collection tube, through passage 3, flow into the sample pool of separation and Culture chamber 2.1 and 2.2 bottoms, make the glutinous sampling of the transfering loop that is arranged in two sample pools originally.The arm 10 of inoculator and 11 and culture plate 5 surface distances bigger, the mutual distance of the every pair of transfering loop 13 is very near, be that arm is away from culture plate, and transfering loop is near culture plate, when mechanical manipulator promotes transfering loop 13 by pilot lever 8, transfering loop 13 can abut against on the substratum of culture plate 5, makes the line better effects if.By about, left and right moving control bar 8, transfering loop 13 can be done vertical shifting and moving horizontally, therefore, transfering loop 13 can move on to the upper end of culture plate 5 from separation and Culture chamber 2.1 and 2.2 bottoms.Simultaneously instrument provides bacterial growth needed environment, as concentration of special gas such as temperature, carbonic acid gas and nitrogen or the like.

Claims

1. novel four sides separation culture device for multi-bacterial sample, it comprises collection tube chamber and two separation and Culture chambers that the sample collection pipe is housed, it is characterized in that: the bottom of collection tube chamber is connected by the sample pool of passage with one of them bottom, separation and Culture chamber, and the sample pool of bottom, two separation and Culture chambers is connected by connecting passage.

2. novel four sides separation culture device for multi-bacterial sample according to claim 1 is characterized in that: in the described sample collection pipe little magnetic patch has been housed.

3. novel four sides separation culture device for multi-bacterial sample according to claim 1, it is characterized in that: two indoor rectangle culture plates that are equipped with respectively of separation and Culture, be filled with solid medium on the culture plate, the indoor streak inoculation device that also is provided with of two separation and Culture, streak inoculation device end is connected with transfering loop, and transfering loop is near culture plate.

4. according to claim 1 or 3 described new bio sample four sides separation culture devices, it is characterized in that: described separation and Culture chamber also comprises the valve gap of upper end, the top engagement of valve gap and separation and Culture chamber.

5. novel four sides separation culture device for multi-bacterial sample according to claim 3, it is characterized in that: described inoculator comprises two arms that become forked, two arms pass valve gap, the upper end connects two arms by crossbeam, the end of two arms is connected with transfering loop respectively, and the middle part of crossbeam is connected with control lever.

6. according to the novel four sides separation culture device for multi-bacterial sample of claim 3, it is characterized in that: described valve gap is a capping, the feather edge of capping is connected with upper end, separation and Culture chamber, be connected with rectangular culture plate in the middle of the bottom of capping, the culture plate dress is inserted into the inside of separation and Culture chamber.