CN101194647B - Novel method for keeping strawberry fresh with polyphenol - Google Patents
Novel method for keeping strawberry fresh with polyphenol Download PDFInfo
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- CN101194647B CN101194647B CN200710015908A CN200710015908A CN101194647B CN 101194647 B CN101194647 B CN 101194647B CN 200710015908 A CN200710015908 A CN 200710015908A CN 200710015908 A CN200710015908 A CN 200710015908A CN 101194647 B CN101194647 B CN 101194647B
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Abstract
The invention discloses a novel method which uses sea weed polyphenol for keeping freshness of the strawberries, which can be used for keeping freshness and storing fresh fruits such as strawberries and the like. The technical essential points comprise utilizing fresh sargassum thunbergii as raw material, organizing homogenate by high speed, dipping and extracting with alcohol of the concentrationof 85% in 5 times, placing on a shaking table under the normal temperature for 12 hours, extracting and filtrating by decompressing, extracting by adopting absolute ether and chloroform respectively,and compressing by an aqueous layer rotating evaporator, namely the sea weed polyphenol is made, and choosing fresh strawberry fruits which are about 80% maturation, rejecting fruits with injury which is caused by disease and insects and mechanical damage, immersing 5mins with sea weed polyphenol solution whose thickness is 5mg /mL under the ambient temperature, drying and storing after packagingby producing perforation polyethylene film. After strawberries are stored for 24 days under the humidity of 90%-95% and temperature of 2+_1DEG C by the method of the invention, the content of the soluble solid matter is reduced by only 6.1%, the hardness is reduced by 21.4%, and the value of the brightness L* of the color and luster index is reduced by 26.2%.
Description
Technical field: the present invention relates to fruit and vegetable preserving method.It specifically is the new method of using the fresh-keeping strawberry of seeweed polyphenol.Adopt the fresh-keeping strawberry of this method, cost is low, and is safe, and good refreshing effect can be self-employed entrepreneur, supermarket, freezer and agricultural products fresh-keeping processing base etc. service is provided.
Background technology:
Strawberry belongs to the berries fruit, delicious flavour, and nutritive value is abundant, but the strawberry respiratory intensity is big, and protective tissue is poor, and preservation and freshness is difficulty relatively, and preservation term and shelf life are all shorter.Be the prolongation strawberry preservation phase at present, alleviate strawberry and rot, the commercial chemical bactericide processing that refrigerate in conjunction with certain of adopting more.But a large amount of uses of chemical bactericide, cause pathogenic microorganism to produce drug resistance, and cause various problems such as environmental pollution, food security, teratogenesis be carcinogenic, and the part chemical bactericide is disabled in some countries, therefore seeks the biological control technology of safety non-toxic and comes fresh-keeping strawberry to seem very necessary.
Marine alga can be subjected to the invasion and attack of marine microorganism in growth course, therefore, it can produce the resistance to various microorganisms in continuous evolutionary process.People have filtered out many antibacteriums and antimycotic marine alga from hundreds of kind marine alga at present.Simultaneously, the antibacterial activity composition in these marine algas is determined gradually that also its active component is various, mainly comprises terpenes, aliphatic acid, bromine phenol, quinhydrones, phloroglucin and alicyclic ring or sulfur heterocyclic compound etc.The brown alga polyphenol is the stronger compound of antibiotic property in the marine alga, and various bacteria, fungi, saccharomycete are had obvious inhibition ability, and common pathogens such as cholera bacteria, staphylococcus aureus, Escherichia coli are all had very strong inhibitory action.Mouse tail algae is the warm temperate zone marine alga, is the coastal common wild species of China.Tide band and low tide band rock in concentrating on, or at land and water or Shi Zhaozhong that high, middle tide is with, have in addition under long period time of low water is exposed to daylight, all can grow.Mouse tail algae is under the jurisdiction of Phaeophyta, Cyclosporae, Sargassaceae, Sargassum.Except that in marine ecosystems, occupying the critical role, in industries such as medicine, health care, aquaculture and chemical industry, also have many developable potentiality.Therefore, be feedstock production seeweed polyphenol antistaling agent with mouse tail algae, not only for strawberry preservation provides new approach, also can improve the added value of algae, short its sustainable development.
At present, do not see the report that uses the fresh-keeping strawberry of seeweed polyphenol as yet.
Summary of the invention:
Purpose of the present invention: the invention provides that a kind of cost is low, safe, the strawberry preservation new method of good refreshing effect.
Technical scheme of the present invention: the separation of (1) brown alga polyphenol.The present invention is a raw material with fresh mouse tail algae, and the high speed tissue homogenate is 85% ethanol with concentration, and the quintuple lixiviate places and places 12h under the shaking table normal temperature, and decompression filters, 35 ℃ of decompression distillation then, and gained is slightly and guides and support.(2) purifying of brown alga polyphenol.Adopt absolute ether and chloroform respectively crude extract to be extracted, after water intaking layer Rotary Evaporators concentrates, be seeweed polyphenol.(3) select the fresh strawberry fruit of about 8 maturations for use, reject disease and pest and mechanical damage fruit, with soaking 5min under seeweed polyphenol solution prepared in (2) (concentration the is 5mg/mL) room temperature, dry, packing the back with the punching polyethylene film is 90%~95% in relative humidity, and temperature is to preserve under 2 ± 1 ℃ of conditions; (4) duration of storage is made regular check on, and in time clears up unusual fruit.
Beneficial effect: because the present invention comes the combine freshness strawberry with natural seaweed polyphenol and low temperature, safety, various problems such as nontoxic, alternative chemical bactericide, anticorrisive agent are used for the anticorrosion and fresh-keeping of strawberry, and environmental pollution, food security, the teratogenesis of having avoided chemical bactericide to cause is carcinogenic.Through the fresh-keeping strawberry of the inventive method, after preserving 24 days under 2 ± 1 ℃ of conditions, the solubility solid content only reduces by 6.1%, and hardness reduces by 21.4%, and brightness L* value reduces by 26.2% in the lustre index.The specific embodiment is as follows:
The ethanol of fresh mouse tail algae → making beating fragmentation → adding 85%, 25 ℃ of lixiviate 12h → suction filtration → Rotary Evaporators concentrate → and absolute ether extraction → chloroform extraction → water layer Rotary Evaporators concentrates → seeweed polyphenol → be mixed with 5mg/mL seeweed polyphenol solution → choose fresh strawberry (7-8 becomes maturity) → removal, polyethylene film parcel → storage (90%~95%, 2 ± 1 ℃ of condition: RH) is dried → punched to cull fruit → selected strawberry with 5mg/mL seeweed polyphenol solution immersion 5min → strawberry.
Claims (2)
1. the method with the fresh-keeping strawberry of seeweed polyphenol is characterized in that with fresh mouse tail algae be raw material, the high speed tissue homogenate, with concentration is 85% ethanol, and the quintuple lixiviate places and places 12h under the shaking table normal temperature, decompress filter, 35 ℃ of decompression distillation then, gained is the crude extract of seeweed polyphenol; Adopt absolute ether and chloroform respectively crude extract to be extracted, after the water intaking layer concentrates with Rotary Evaporators, be the seeweed polyphenol of purifying; Select medium well fresh strawberry fruit for use, reject disease and pest and mechanical damage fruit, and at room temperature be soaked in the seeweed polyphenol solution 5min of above-mentioned purifying, dry, pack the back storage with the punching polyethylene film again; Duration of storage is made regular check on, and in time clears up unusual fruit.
2. the method with the fresh-keeping strawberry of seeweed polyphenol as claimed in claim 1 is characterized in that employed seeweed polyphenol concentration is 5mg/mL, and the holding conditions of handling the back strawberry is a relative humidity 90%~95%, and temperature is 2 ± 1 ℃.
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CN200710015908A CN101194647B (en) | 2007-06-01 | 2007-06-01 | Novel method for keeping strawberry fresh with polyphenol |
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CN200710015908A CN101194647B (en) | 2007-06-01 | 2007-06-01 | Novel method for keeping strawberry fresh with polyphenol |
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CN101194647A CN101194647A (en) | 2008-06-11 |
CN101194647B true CN101194647B (en) | 2010-05-19 |
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CN200710015908A Expired - Fee Related CN101194647B (en) | 2007-06-01 | 2007-06-01 | Novel method for keeping strawberry fresh with polyphenol |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102726808A (en) * | 2011-04-07 | 2012-10-17 | 浙江省海洋开发研究院 | Method for using alga polyphenol to prevent blackening of seawater shrimps |
CN102228079B (en) * | 2011-05-27 | 2012-10-31 | 昆明理工大学 | Active color protecting antistaling agent of wild edible fungus and preparation method thereof |
CN103054131B (en) * | 2011-09-08 | 2014-10-01 | 浙江海洋学院 | Preparation method of high-purity Caulerpa racemesa polyphenol and application thereof to fresh-keeping of shrimp |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1202334A (en) * | 1998-05-28 | 1998-12-23 | 中国科学院海洋研究所 | Preparation of seeweed polyphenol antioxidation agent and its applying method |
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2007
- 2007-06-01 CN CN200710015908A patent/CN101194647B/en not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN1202334A (en) * | 1998-05-28 | 1998-12-23 | 中国科学院海洋研究所 | Preparation of seeweed polyphenol antioxidation agent and its applying method |
Non-Patent Citations (6)
Title |
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林超等.鼠尾藻中褐藻多酚化合物的抑菌活性研究.海洋科学第30卷 第3期.2006,第30卷(第3期),第94-97页. |
林超等.鼠尾藻中褐藻多酚化合物的抑菌活性研究.海洋科学第30卷 第3期.2006,第30卷(第3期),第94-97页. * |
魏玉西等.褐藻中多酚化合物的研究进展.海洋科学第26卷 第10期.2002,第26卷(第10期),第18-20页. |
魏玉西等.褐藻中多酚化合物的研究进展.海洋科学第26卷 第10期.2002,第26卷(第10期),第18-20页. * |
魏玉西等.褐藻中高相对分子质量褐藻多酚的抗氧化活性研究.中草药第34卷 第4期.2003,第34卷(第4期),第317-319页. |
魏玉西等.褐藻中高相对分子质量褐藻多酚的抗氧化活性研究.中草药第34卷 第4期.2003,第34卷(第4期),第317-319页. * |
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