CN101180984A - Edible mythic fungus bacterium ball milk and preparation method thereof - Google Patents
Edible mythic fungus bacterium ball milk and preparation method thereof Download PDFInfo
- Publication number
- CN101180984A CN101180984A CNA2007101152478A CN200710115247A CN101180984A CN 101180984 A CN101180984 A CN 101180984A CN A2007101152478 A CNA2007101152478 A CN A2007101152478A CN 200710115247 A CN200710115247 A CN 200710115247A CN 101180984 A CN101180984 A CN 101180984A
- Authority
- CN
- China
- Prior art keywords
- milk
- edible
- mythic fungus
- fungus bacterium
- bacterium ball
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Non-Alcoholic Beverages (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses edible ganoderma lucidum fungus ball milk, which is blended by edible ganoderma lucidum fungus fermentation broth and pure milk in a volume portion of 1:1 to 4; wherein, the ganoderma lucidum fungus fermentation broth refers to ganoderma lucidum fungus fermentation broth of 3 to 12 fungus balls with the diameter of 3 to 8 mm. The ganoderma lucidum fungus ball milk of the invention integrates milk nutrition and ganoderma lucidum functional components, and has the advantages of pure taste and rich nutrition; the ganoderma lucidum fungus ball milk shows effectiveness on preventing diseases, strengthening physical fitness and improving human immunity, and belongs to preference diet excellent products; the ganoderma lucidum fungus ball milk of the invention is characterized by simple technique and low cost, and is particularly easy to produce on scale, and promotion and implement.
Description
Technical field
The present invention relates to microbial fermentation technology and beverage product technical field, exactly relate to a kind of edible mythic fungus bacterium ball milk and preparation method thereof.
Background technology
Glossy ganoderma " property flat, bitter, nontoxic " can " the beneficial motive ", " pacifying smart soul ", " hard muscles and bones ", " liver-kidney tonifying ", is high-grade medicine-food two-purpose fungi precious in state's Drug Storage.Through the ages be referred to as celestial grass, auspicious grass, refreshing grass, enjoy high reputation among the people.Modern age a large amount of artificial cultivation techniques success, changed rare, the shortage situation of glossy ganoderma.But because lignified ganoderma lucidum fruitbody skin, shell are tough and tensile, can not directly eat, can't satisfy the active demand of current social people glossy ganoderma.The mouthfeel and the nutritive value of milk generally acknowledge, but that the function that glossy ganoderma is promoted health is a milk is irreplaceable.Glossy ganoderma and milk are prepared a kind of glossy ganoderma milk, so can make glossy ganoderma enter huge numbers of families with milk as carrier, people not only can enjoy the health-care effect of glossy ganoderma easily, have also increased the kind of function milk simultaneously, have improved the added value of dairy products.Although existing at present multinomial patent disclosure so-called glossy ganoderma milk, but it is a raw material with ganoderma lucidum fruitbody and lucidum spore powder mainly, form, component difference because of used glossy ganoderma, cause the content of its pharmacological component maybe can not guarantee entirely, and its processing technology complexity, cost is too high, product have the precipitation and taste not good.
The ganoderma lucidum mycelium of Liquid Culture contains a large amount of pharmacological components---ganoderma lucidum crude polysaccharide, and described polyoses content is 6 times of the aging fructification of glossy ganoderma, is 2.8 times of lucidum spore powder.If particularly the mythic fungus bacterium ball fermentation liquid is directly hybrid packed with plain chocolate with pure ganoderma lucidum mycelium, and is directly perceived with its form, comprehensive nutrition is with low cost, is convenient to operate and implementation feature, may have more tempting market prospects.By retrieval, yet there are no with pure ganoderma lucidum mycelium directly and plain chocolate be mixed with the report of mythic fungus bacterium ball milk.
Summary of the invention
At the deficiency of existing dairy products kind, the problem to be solved in the present invention provides a kind of edible mythic fungus bacterium ball milk and preparation method thereof, and is incomplete with the active principle content that improves glossy ganoderma milk, reaches processed complex, defect of high cost.
Edible mythic fungus bacterium ball milk of the present invention is cooperated with plain chocolate by the edible mythic fungus bacterium fermentation liquid to form, and it is characterized in that described edible mythic fungus bacterium fermentation liquid is meant every milliliter of mythic fungus bacterium ball fermentation liquid that contains 3～12 diameter 3～8mm bacterium balls; Described plain chocolate is dew fresh milk; And the edible mythic fungus bacterium ball fermentation liquid is 1: 1～4 with the volume ratio that cooperates of dew fresh milk.
Wherein, described edible mythic fungus bacterium fermentation liquid preferably is meant every milliliter of mythic fungus bacterium ball fermentation liquid that contains 5～8 diameter 5～7mm bacterium balls; And the edible mythic fungus bacterium ball fermentation liquid cooperated volume ratio preferred 1: 3 with dew fresh milk.
In the above-mentioned edible mythic fungus bacterium ball milk, described edible mythic fungus bacterium fermentation liquid is obtained by following method:
(1) bacterial classification is selected: glossy ganoderma Ganoderma lucidum (Leyss.ex Fr.) Karst.CCTCC No.AF92001, or glossy ganoderma Ganoderma lucidum (W.Curtis:Fries) Karsten CGMCC No.5.706, or glossy ganoderma Ganoderma lucidum (W.Curtis:Fries) Karsten CGMCC No.5.743, or glossy ganoderma Ganodermalucidum (W.Curtis:Fries) Karsten CGMCC No.5.744, or glossy ganoderma Ganoderma lucidum (W.Curtis:Fries) Karsten CGMCC No.5.765;
(2) inclined-plane is cultivated: above-mentioned bacterial classification inoculation on the PDA slant medium, 26 ℃～30 ℃, was cultivated 4～6 days;
(3) seed culture:, under aseptic condition, use inoculation clasp joint 2～4cm with the bacterial strain that step (2) is cultivated
2In the 1000ml triangular flask that contains 200～400mL CD-06 nutrient solution, with 26 ℃～30 ℃ of temperature, 160～200 rev/mins of shaking speed were cultivated 4～6 days, treated that nutrient solution is limpid, the bacterium ball forms and promptly makes seed liquor;
(4) enlarge cultivation: with the inoculum concentration of 10%～20% volume ratio, the seed liquor of step (3) is inoculated in the sterilized 5000 milliliters of triangular flasks that contain 2000mL CS 15 nutrient solutions, with 26 ℃～30 ℃ of temperature, 120～160 rev/mins of shaking table rotary speeies, cultivated 3～5 days, when treating the limpid and every milliliter of bacterium ball that contains 3～12 diameter 3～8mm of mastoid process or barbed mythic fungus bacterium ball suspension, clear-cut, nutrient solution, collect fermentation liquid, promptly get and be used for the edible mythic fungus bacterium ball fermentation liquid that cooperates with milk;
Wherein: above-mentioned PDA slant medium preparation method is: get potato 200 gram, the peeling section adds 1 liter in water and boiled 30 minutes, removes piece and will remove piece filtrate moisturizing to 1 liter, adds glucose 20 grams, agar 15 grams, and potassium dihydrogen phosphate 3 grams, magnesium sulfate 1.5 restrains natural pH;
Above-mentioned CD-06 nutrient solution preparation method is: remove Pishan medicine 60 grams, cut fritter, boiled 10 minutes, the cooling back adds 20 gram glucose with the soy bean milk making machine making beating, supplies 1.0 liters with running water;
Above-mentioned CS-15 nutrient solution preparation method is: remove Pishan medicine 150 gram, stripping and slicing, boiled 10 minutes, the cooling back adds 40 gram sucrose with the soy bean milk making machine making beating, supplies 1.0 liters with running water.
Above-mentioned culture medium also can separately or be arranged in pairs or groups and use potato, corn flour, wheat bran except that with the Chinese yam.
Preferred 28 ℃～30 ℃ of above-mentioned steps (2), (3), (4) described cultivation temperature.
Preferred 170～190 rev/mins of the described shaking speed of above-mentioned steps (3).
Preferred 130～150 rev/mins of the described shaking speed of above-mentioned steps (4).
The preparation method of edible mythic fungus bacterium ball milk of the present invention, it is characterized in that, with described edible mythic fungus bacterium ball fermentation liquid in volume ratio, ratio in 1: 1～4 cooperates with dew fresh milk, mixing, business demand mode with milk becomes dress, sealing then, carry out pasteurization, promptly make described edible mythic fungus bacterium ball milk.
Among the preparation method of above-mentioned edible mythic fungus bacterium ball milk, described edible mythic fungus bacterium ball fermentation liquid cooperated volume ratio preferred 1: 3 with dew fresh milk.
Wherein, described edible mythic fungus bacterium ball fermentation liquid is meant every milliliter of glossy ganoderma bacterium fermentation liquid that contains 3～12 diameter 3～8mm bacterium balls; Preferably be meant every milliliter of glossy ganoderma bacterium fermentation liquid that contains 5～8 diameter 5～7mm bacterium balls.
Edible mythic fungus bacterium ball milk of the present invention is mainly cooperated with plain chocolate by edible mythic fungus bacterium ball and forms, described edible mythic fungus bacterium ball is a fluid nutrient medium of selecting for use Chinese yam and glucose or sucrose to form, insert the glossy ganoderma bacterium, cultivate the mythic fungus bacterium ball that generates at suitable condition bottom fermentation.Edible mythic fungus bacterium ball of the present invention is made up of pure ganoderma lucidum mycelium, and need not reprocess, can be directly and plain chocolate hybrid packed, its form is directly perceived, comprehensive nutrition is with low cost, and is workable, is convenient to implement industrialization.
Edible mythic fungus bacterium ball milk of the present invention has had both all advantages of deep glossy ganoderma fermenting mycelia and milk, and the mythic fungus bacterium ball morphological appearance is tempting, and nutrition composition is comprehensive, and mouthfeel is good, easily digest and assimilate.Especially edible mythic fungus bacterium ball milk has adopted pasteurization technology, can more reservation mythic fungus bacterium ball milks in SOD enzyme isoreactivity composition, make health-care efficacy better; And manufacture craft is simple, cost is low, is easy to promotion and implementation.
Characteristics of the present invention are:
Not directly ganoderma lucidum fruitbody or ganoderma lucidum fermented liquid to be extracted separated component adding milk, but with scientific method High Density Cultivation mythic fungus bacterium ball, the mythic fungus bacterium ball of mycelial concentration far above the full liquid of common glossy ganoderma fermentation cooperated with commodity milk according to the required ratio of Human Physiology, health care is combined with the nutraceutical science, strengthened the health care of milk, the convenience of glossy ganoderma, reliable, economical edible way are provided simultaneously.
The culture medium that the present invention uses is the pure natural culture medium, and key component is Chinese yam (DioscoreaopposttaThunb.), and another name Huai Shan, Huai Shan, Chinese yam rhizome, RHIIZOMA DIOSCOREAE frOm Henan of China, Chinese yam, dioscorea japonica are medicine, food dual purpose plant.It has effects such as tonifying spleen, beneficial lung kidney with piece root hyoscine.Cure mainly under spleen bone weakness, diarrhoea, protracted dysentery, the band, cough due to deficiency of the lung, diabetes, frequent micturition, quench one's thirst, disease such as indigestion, chronic enteritis.Therefore, the lifting that the application of pure natural culture medium of the present invention also can be indirect the health care of mythic fungus bacterium ball milk.
Modern medicine study proves, glossy ganoderma truly has outside the significant curative effect cancer, cerebral hemorrhage, the heart disease of the human three big causes of the death, can also treat more than 40 kind of diseases such as asthma, hepatitis, stomach trouble, ephritis, impotence, insomnia, constipation, night sweat, diabetes, hypertension, high fat of blood, neurasthenia, hypertrophy of the prostate, white blood cell minimizing.Mythic fungus bacterium ball milk of the present invention integrates cow's milk nutrition and glossy ganoderma functional component, and good taste is nutritious, to prevent disease, builds up health, and improves body immunity, has obvious curative effects, is that people must eat every day, the first-selected good merchantable brand that every meal must have.
The active ingredient of glossy ganoderma is abundant, and pharmacologically active is extensive, and existing medical sci-tech worker's in recent years research report is concluded and is summarized as follows:
GL-B is one of the most effective composition in the glossy ganoderma, can improve immunity of organisms, improves body's hypoxia tolerance, eliminates free radical, suppresses tumour, and anti-radiation improves liver, marrow, blood synthetic DNA, RNA, protein ability, life-saving.
Ganoderic acid has strong pharmacologically active, has pain relieving, calmness, inhibition histamine to discharge, detoxify, protect the liver, kill functions such as tumour cell, is one of main active ingredient of glossy ganoderma.
Glossy ganoderma has multiple adenosine derivative, and its pharmacologically active is to reduce blood viscosity, suppresses platelet aggregation in the body, can improve hemoglobin 2, the content of 3-diphosphonic acid glycosides oil can improve the blood oxygen ability and quicken blood microcirculation, improves the oxygen delivery capacity of blood to the heart, brain.
The health care of milk is summarized as follows:
Casein in the ※ milk contains 10% phosphorus, and the brain development that promotes the child is played crucial effects.
Containing potassium in the ※ milk makes the arterial blood tube wall flow at great speed at blood to keep stable when high, make risk of stroke reduce half.
※ milk can stop metallic lead poisonous in the absorption of human body food and cadmium, has slight function of detoxification.
Contain 5% lactose nearly in the ※ milk, can promote the absorption of human body, strengthen enterocinesia, promote to drain calcium and iron.
※ sour milk and defatted milk can strengthen the immune system function, stop tumour cell to increase.
Vitamin B2 in the ※ milk can improve eyesight.
The ※ drink milk also can prevent artery sclerosis.
※ milk also has certain curative effect to gastric and duodenal ulcer.
Cruel propylhomoserin in the ※ milk can promote happy hormone serum hormone growth.
Iron in the ※ milk, copper and vitamin A have beautification function, make skin keep smooth and plentiful.
Magnesium in the ※ milk can be alleviated heart and nervous system fatigue.
Zinc in the ※ milk can promote that wound heals quickly.
Calcium in the ※ milk can strengthen the bone tooth, makes the bone wasting disease reduce half, promotes infant and teen-age intellectual development and physiological development.
Protein, enzyme peptide, hormone etc. all have the activity of regulating body function in the ※ milk, have prevention to infect, regulate body's immunity and antibacterial activity, can prevent multiple disease.
The specific embodiment
Embodiment 1,
1, cryopreserved lucidum strain (glossy ganoderma Ganoderma lucidum (Leyss.ex Fr.) Karst.CCTCCNo.AF92001) is taken out, at room temperature put after 2 days and be transferred on the PDA slant medium.
2, constant temperature is 28 ℃, cultivates 4～6 days, and white Ganoderma lucidum mycelium covers with the inclined-plane and can use.
3, make the CD-06 nutrient solution, in the 1000ml triangular flask of packing into, the 200ml/ bottle, 8 layers of gauze of bottleneck lid are used steam sterilizing, and 121 ℃, pressure 1kg/cm
2, pressurize 40 minutes.After the sterilization, naturally cool to 30 ℃ and promptly can be used for inoculation.
4, in above-mentioned every triangular flask, insert 2～4cm
2Fresh pure glossy ganoderma bacterium inclined-plane, with 28 ℃～30 ℃ of cultivation temperature, 200 rev/mins of rotary shaker rotating speeds were cultivated 4～6 days, treated that nutrient solution is limpid, the bacterium ball forms and promptly make seed liquor.
5, make the CS-15 nutrient solution, in the 5000 milliliters of triangular flasks of packing into, the 2000ml/ bottle, 8 layers of gauze of bottleneck lid are used steam sterilizing, and 121 ℃, pressure 1kg/cm
2, pressurize 50 minutes.After the sterilization, naturally cool to 30 ℃ and promptly can be used for inoculation.
6, with the inoculum concentration of 10% volume ratio, the liquid lucidum strain in the long good 1000ml triangular flask is inserted in sterilized 5000 milliliters of triangular flasks, with 28 ℃～30 ℃ of cultivation temperature, 160 rev/mins of shaking table rotary speeies were cultivated 3～5 days; Treat that mastoid process or barbed mythic fungus bacterium ball suspension, clear-cut, nutrient solution are limpid and every milliliter when containing 3～12 diameter 3～8mm bacterium balls, collect fermentation liquid, promptly get and be used for the edible mythic fungus bacterium ball fermentation liquid that cooperates with milk.
※ PDA inclined-plane makes: get potato 200 grams, the peeling section adds 1 liter in water and boiled 30 minutes, removes piece and will supply 1 liter except that piece filtrate, adds glucose 20 grams, agar 15 grams, potassium dihydrogen phosphate 3 grams, magnesium sulfate 1.5 grams, natural pH.After dissolving mixing, above-mentioned culture medium heating is divided in the test tube of 18 millimeters of diameters, 8 milliliters of loading amounts, and cooling back test tube mouth adds tampon, uprightly is placed in the pressure cooker after packing with brown paper or newspaper again, uses steam sterilizing, and 121 ℃, pressure 1kg/cm
2, pressurize 30 minutes is treated to take out after pressure reduces to zero naturally, sets inclined-plane (being about 1/2～3/5 of test tube length is advisable) while hot, cools off standby.
※ CD-06 nutrient solution is made: remove Pishan medicine 60 grams, cut fritter, boiled 10 minutes, the cooling back adds 20 gram glucose with the soy bean milk making machine making beating, supplies 1.0 liters with running water.
※ CS-15 nutrient solution is made: remove Pishan medicine 150 grams, stripping and slicing, boiled 10 minutes, the cooling back adds 40 gram sucrose with the soy bean milk making machine making beating, supplies 1.0 liters with running water.
Embodiment 2,
1, make the CD-06 nutrient solution, in the 1000ml triangular flask of packing into, the 300ml/ bottle, 8 layers of gauze of bottleneck lid are used steam sterilizing, and 121 ℃, pressure 1kg/cm
2, pressurize 40 minutes.After the sterilization, naturally cool to 30 ℃ and promptly can be used for inoculation.
2, in above-mentioned every triangular flask, insert 2～4cm
2Fresh pure glossy ganoderma Ganoderma lucidum (W.Curtis:Fries) Karsten CGMCC No.5.706 bacterium inclined-plane, with 30 ℃ of cultivation temperature, 180 rev/mins of rotary shaker rotating speeds were cultivated 4～6 days, treated that nutrient solution is limpid, the bacterium ball forms and promptly makes seed liquor.
3, make the CS-15 nutrient solution, in the 5000 milliliters of triangular flasks of packing into, the 2000ml/ bottle, 8 layers of gauze of bottleneck lid are used steam sterilizing, and 121 ℃, pressure 1kg/cm
2, pressurize 50 minutes.After the sterilization, naturally cool to 30 ℃ and promptly can be used for inoculation.
4, will grow one bottle of liquid lucidum strain in the good 1000ml triangular flask, insert in sterilized 5000 milliliters of triangular flasks, inoculum concentration about 15%, with 30 ℃ of cultivation temperature, 150 rev/mins of shaking speed were cultivated 3～5 days, when treating the limpid and every milliliter of bacterium ball that contains 3～12 diameter 3～8mm of mastoid process or barbed mythic fungus bacterium ball suspension, clear-cut, nutrient solution, collect fermentation liquid, promptly get and be used for the edible mythic fungus bacterium ball fermentation liquid that cooperates with milk.
Embodiment 3,
1, bacterial classification is selected: glossy ganoderma Ganoderma lucidum (W.Curtis:Fries) Karsten.CGMCC No.5.744;
2, the inclined-plane is cultivated: bacterial classification inoculation on the PDA slant medium, 28 ℃～30 ℃, was cultivated 4～6 days;
3, make the CD-06 nutrient solution, in the 1000ml triangular flask of packing into, the 320ml/ bottle, 8 layers of gauze of bottleneck lid are used steam sterilizing, and 121 ℃, pressure 1kg/cm
2, pressurize 40 minutes.After the sterilization, naturally cool to 30 ℃ and promptly can be used for inoculation.
4, in above-mentioned every triangular flask, insert 2～4cm
2Fresh pure glossy ganoderma bacterium inclined-plane, with 29 ℃ of cultivation temperature, 170 rev/mins of rotary shaker rotating speeds were cultivated 4～6 days, treated that nutrient solution is limpid, the bacterium ball forms and promptly make seed liquor.
5, make the CS-15 nutrient solution, in the 5000 milliliters of triangular flasks of packing into, the 2000ml/ bottle, 8 layers of gauze of bottleneck lid are used steam sterilizing, and 121 ℃, pressure 1kg/cm
2, pressurize 50 minutes.After the sterilization, naturally cool to 30 ℃ and promptly can be used for inoculation.
6, will grow one bottle of liquid lucidum strain in the good 1000ml triangular flask, insert in sterilized 5000 milliliters of triangular flasks, inoculum concentration about 16%, with 29 ℃ of cultivation temperature, 140 rev/mins of shaking speed were cultivated 3～5 days, treated that mastoid process or barbed mythic fungus bacterium ball suspension, clear-cut, nutrient solution are limpid and every milliliter when containing 3～12 diameter 3～8mm bacterium balls, collect fermentation liquid, promptly get and be used for the edible mythic fungus bacterium ball fermentation liquid that cooperates with milk.
Embodiment 4,
1, bacterial classification is selected: glossy ganoderma Ganoderma lucidum (W.Curtis:Fries) Karsten.CGMCC No.5.743;
2, the inclined-plane is cultivated: bacterial classification inoculation on the PDA slant medium, 28 ℃～30 ℃, was cultivated 4～6 days;
3, make the CD-06 nutrient solution, in the 1000ml triangular flask of packing into, the 360ml/ bottle, 8 layers of gauze of bottleneck lid are used steam sterilizing, and 121 ℃, pressure 1kg/cm
2, pressurize 40 minutes.After the sterilization, naturally cool to 30 ℃ and promptly can be used for inoculation.
4, in above-mentioned every triangular flask, insert 2～4cm
2Fresh pure glossy ganoderma bacterium inclined-plane, 26 ℃ of cultivation temperature, 160 rev/mins of rotary shaker rotating speeds were cultivated 4～6 days, treated that nutrient solution is limpid, the bacterium ball forms and promptly make seed liquor.
5, make the CS-15 nutrient solution, in the 5000 milliliters of triangular flasks of packing into, the 2000ml/ bottle, 8 layers of gauze of bottleneck lid are used steam sterilizing, and 121 ℃, pressure 1kg/cm
2, pressurize 50 minutes.After the sterilization, naturally cool to 30 ℃ and promptly can be used for inoculation.
6, one bottle of liquid lucidum strain in the good 1000ml triangular flask be will grow, about 18%, the 26 ℃ of cultivation of inoculum concentration, 130 rev/mins of shaking speed inserted in sterilized 5000 milliliters of triangular flasks.Cultivated 3～5 days, and treated that mastoid process or barbed mythic fungus bacterium ball suspension, clear-cut, nutrient solution were limpid and every milliliter when containing 3～12 diameter 3～8mm bacterium balls, collect fermentation liquid, promptly get and be used for the edible mythic fungus bacterium ball fermentation liquid that cooperates with milk.
Embodiment 5,
(1) bacterial classification is selected: glossy ganoderma Ganoderma lucidum (W.Curtis:Fries) Karsten.CGMCC No.5.765;
(2) inclined-plane is cultivated: bacterial classification inoculation on the PDA slant medium, 28 ℃～30 ℃, was cultivated 4～6 days;
(3) seed culture:, under aseptic condition, use inoculation clasp joint 2～4cm with the bacterial strain that step (2) is cultivated
2In the 1000ml triangular flask that contains 400mL CD-06 nutrient solution, with 28 ℃～30 ℃ of temperature, 190 rev/mins of shaking speed were cultivated 4～6 days, treated that nutrient solution is limpid, the bacterium ball forms and promptly makes seed liquor;
(4) enlarge cultivation: the inoculum concentration of volume ratio with 20%, the fermentation liquid of step (4) is inoculated in the sterilized 5000 milliliters of triangular flasks that contain the 2000mLCS-15 nutrient solution, with 28 ℃～30 ℃ of temperature, 120 rev/mins of shaking table rotary speeies, cultivated 3～5 days, treat that mastoid process or barbed mythic fungus bacterium ball suspension, clear-cut, nutrient solution are limpid and every milliliter when containing 3～12 diameter 3～8mm bacterium balls, collect fermentation liquid, promptly get and be used for the edible mythic fungus bacterium ball fermentation liquid that cooperates with milk;
Wherein: above-mentioned PDA slant medium preparation method is: get potato 200 gram, the peeling section adds 1 liter in water and boiled 30 minutes, will remove piece filtrate moisturizing to 1 liter, adds glucose 20 grams, agar 15 grams, and potassium dihydrogen phosphate 3 grams, magnesium sulfate 1.5 restrains natural pH;
Above-mentioned CD-06 nutrient solution preparation method is: remove Pishan medicine 60 grams, cut fritter, boiled 10 minutes, the cooling back adds 20 gram glucose with the soy bean milk making machine making beating, supplies 1.0 liters with running water;
Above-mentioned CS-15 nutrient solution preparation method is: remove Pishan medicine 150 gram, stripping and slicing, boiled 10 minutes, the cooling back adds 40 gram sucrose with the soy bean milk making machine making beating, supplies 1.0 liters with running water.
Embodiment 6, preparation mythic fungus bacterium ball milk
With the edible mythic fungus bacterium ball fermentation liquid of embodiment 5 preparation in volume ratio, by 1: 3 ratio cooperate with dew fresh milk, mixing, the business demand mode with milk becomes to adorn, seals then, carries out pasteurization, promptly makes described edible mythic fungus bacterium ball milk.
Embodiment 7, preparation mythic fungus bacterium ball milk
With the edible mythic fungus bacterium ball fermentation liquid of embodiment 3 preparation in volume ratio, by 1: 2 ratio cooperate with dew fresh milk, mixing, the business demand mode with milk becomes to adorn, seals then, carries out pasteurization, promptly makes described edible mythic fungus bacterium ball milk.
Embodiment 8, preparation mythic fungus bacterium ball milk
With the edible mythic fungus bacterium ball fermentation liquid of embodiment 4 preparation in volume ratio, by 1: 4 ratio cooperate with dew fresh milk, mixing, the business demand mode with milk becomes to adorn, seals then, carries out pasteurization, promptly makes described edible mythic fungus bacterium ball milk.
Embodiment 9, preparation mythic fungus bacterium ball milk
With the edible mythic fungus bacterium ball fermentation liquid of embodiment 1 preparation in volume ratio, by 1: 1 ratio cooperate with dew fresh milk, mixing, the business demand mode with milk becomes to adorn, seals then, carries out pasteurization, promptly makes described edible mythic fungus bacterium ball milk.
Claims (10)
1. edible mythic fungus bacterium ball milk is cooperated with plain chocolate by the edible mythic fungus bacterium fermentation liquid to form, and it is characterized in that described edible mythic fungus bacterium fermentation liquid is meant every milliliter of mythic fungus bacterium ball fermentation liquid that contains 3～12 diameter 3～8mm bacterium balls; Described plain chocolate is dew fresh milk; And the edible mythic fungus bacterium ball fermentation liquid is 1: 1～4 with the volume ratio that cooperates of dew fresh milk.
2. edible mythic fungus bacterium ball milk as claimed in claim 1 is characterized in that, described edible mythic fungus bacterium fermentation liquid is meant every milliliter of mythic fungus bacterium ball fermentation liquid that contains 5～8 diameter 5～7mm bacterium balls; And the edible mythic fungus bacterium ball fermentation liquid is 1: 3 with the volume ratio that cooperates of dew fresh milk.
3. edible mythic fungus bacterium ball milk as claimed in claim 1 or 2 is characterized in that, described edible mythic fungus bacterium fermentation liquid is obtained by following method:
(1) bacterial classification is selected: glossy ganoderma Ganoderma lucidum (Leyss.ex Fr.) Karst.CCTCC No.AF92001, or glossy ganoderma Ganoderma lucidum (W.Curtis:Fries) Karsten CGMCC No.5.706, or glossy ganoderma Ganoderma lucidum (W.Curtis:Fries) Karsten CGMCC No.5.743, or glossy ganoderma Ganodermalucidum (W.Curtis:Fries) Karsten CGMCC No.5.744, or glossy ganoderma Ganoderuma lucidum (W.Curtis:Fries) Karsten CGMCC No.5.765;
(2) inclined-plane is cultivated: above-mentioned bacterial classification inoculation on the PDA slant medium, 26 ℃～30 ℃, was cultivated 4～6 days;
(3) seed culture:, under aseptic condition, use inoculation clasp joint 2～4cm with the bacterial strain that step (2) is cultivated
2In the 1000ml triangular flask that contains 200～400mL CD-06 nutrient solution, with 26 ℃～30 ℃ of temperature, 160～200 rev/mins of shaking speed were cultivated 4～6 days, treated that nutrient solution is limpid, the bacterium ball forms and promptly makes seed liquor;
(4) enlarge cultivation: with the inoculum concentration of 10%～20% volume ratio, the seed liquor of step (3) is inoculated in the sterilized 5000 milliliters of triangular flasks that contain 2000mL CS-15 nutrient solution, with 26 ℃～30 ℃ of temperature, 120～160 rev/mins of shaking table rotary speeies, cultivated 3～5 days, when treating the limpid and every milliliter of bacterium ball that contains 3～12 diameter 3～8mm of mastoid process or barbed mythic fungus bacterium ball suspension, clear-cut, nutrient solution, collect fermentation liquid, promptly get and be used for the edible mythic fungus bacterium ball fermentation liquid that cooperates with milk;
Wherein: above-mentioned PDA slant medium preparation method is: get potato 200 gram, the peeling section adds 1 liter in water and boiled 30 minutes, removes piece and will remove piece filtrate moisturizing to 1 liter, adds glucose 20 grams, agar 15 grams, and potassium dihydrogen phosphate 3 grams, magnesium sulfate 1.5 restrains natural pH;
Above-mentioned CD-06 nutrient solution preparation method is: remove Pishan medicine 60 grams, cut fritter, boiled 10 minutes, the cooling back adds 20 gram glucose with the soy bean milk making machine making beating, supplies 1.0 liters with running water;
Above-mentioned CS-15 nutrient solution preparation method is: remove Pishan medicine 150 gram, stripping and slicing, boiled 10 minutes, the cooling back adds 40 gram sucrose with the soy bean milk making machine making beating, supplies 1.0 liters with running water.
4. edible mythic fungus bacterium ball milk as claimed in claim 3 is characterized in that, step (2), (3), (4) described cultivation temperature are 28 ℃～30 ℃.
5. edible mythic fungus bacterium ball milk as claimed in claim 3 is characterized in that, the described shaking speed of step (3) is 170～190 rev/mins.
6. edible mythic fungus bacterium ball milk as claimed in claim 3 is characterized in that, the described shaking speed of step (4) is 130～150 rev/mins.
7. the preparation method of the described edible mythic fungus bacterium ball milk of claim 1, it is characterized in that, with described edible mythic fungus bacterium ball fermentation liquid in volume ratio, ratio in 1: 1～4 cooperates with dew fresh milk, mixing, business demand mode with milk becomes dress, sealing then, carry out pasteurization, promptly make described edible mythic fungus bacterium ball milk.
8. as the preparation method of edible mythic fungus bacterium ball milk as described in the claim 7, it is characterized in that described edible mythic fungus bacterium ball fermentation liquid is 1: 3 with the volume ratio that cooperates of dew fresh milk.
9. as the preparation method of edible mythic fungus bacterium ball milk as described in the claim 7, it is characterized in that described edible mythic fungus bacterium ball fermentation liquid is meant every milliliter of glossy ganoderma bacterium fermentation liquid that contains 3～12 diameter 3～8mm bacterium balls.
10. as the preparation method of edible mythic fungus bacterium ball milk as described in the claim 9, it is characterized in that described edible mythic fungus bacterium ball fermentation liquid is meant every milliliter of glossy ganoderma bacterium fermentation liquid that contains 5～8 diameter 5～7mm bacterium balls.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101152478A CN101180984B (en) | 2007-12-12 | 2007-12-12 | Edible mythic fungus bacterium ball milk and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101152478A CN101180984B (en) | 2007-12-12 | 2007-12-12 | Edible mythic fungus bacterium ball milk and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101180984A true CN101180984A (en) | 2008-05-21 |
| CN101180984B CN101180984B (en) | 2011-04-06 |
Family
ID=39446517
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007101152478A Expired - Fee Related CN101180984B (en) | 2007-12-12 | 2007-12-12 | Edible mythic fungus bacterium ball milk and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101180984B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104082740A (en) * | 2014-06-25 | 2014-10-08 | 北京赛升药业股份有限公司 | Composition for interfering or treating diabetes and preparation method of composition |
| CN104996729A (en) * | 2014-04-21 | 2015-10-28 | 浙江科技学院 | Comprehensive utilization technology of radix ophiopogonis decoction dregs |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1536069B (en) * | 2003-04-10 | 2012-02-15 | Ap营养药品私人有限公司 | Method for large-scale production of catepillar fungus and lucid ganoderma |
| CN1543823A (en) * | 2003-11-17 | 2004-11-10 | 吉林省乳业集团广泽有限公司 | Ganoderma lucidum polysaccharide series dairy product and production process thereof |
| CN1836530A (en) * | 2005-03-25 | 2006-09-27 | 周瑞 | Lucid ganoderma dairy produce series and its production method |
-
2007
- 2007-12-12 CN CN2007101152478A patent/CN101180984B/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104996729A (en) * | 2014-04-21 | 2015-10-28 | 浙江科技学院 | Comprehensive utilization technology of radix ophiopogonis decoction dregs |
| CN104082740A (en) * | 2014-06-25 | 2014-10-08 | 北京赛升药业股份有限公司 | Composition for interfering or treating diabetes and preparation method of composition |
| CN104082740B (en) * | 2014-06-25 | 2016-08-24 | 天津泰创生物科技有限公司 | A kind of have intervention or the composition improving diabetes and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101180984B (en) | 2011-04-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104757112B (en) | A kind of special flavor functional yoghourt and preparation method thereof | |
| CN109805232A (en) | Jujube three-stage probiotics fermention produces Low acid micro-ecological foods | |
| CN105249100A (en) | Production method of fermented fruit and vegetable juice and Chinese rice wine composited function beverage | |
| CN104305427A (en) | Grape and red tea fungus drink | |
| CN107125761A (en) | Root of kudzu vine extract, root of kudzu vine Yoghourt and preparation method thereof | |
| CN107258999A (en) | A kind of agaric fungus fermented tea preparation method | |
| CN107549309B (en) | Fruity selenium-rich cordyceps militaris yoghourt product and preparation method thereof | |
| CN102726665B (en) | Method for producing corn flour by the solid state fermentation of maize pulp by using edible fungi | |
| CN101406214A (en) | Golden fungus yoghourt with nutrition health-care functions and preparation method thereof | |
| CN101180985B (en) | Edible pupa aweto bacterium ball milk and preparation method thereof | |
| CN109452634B (en) | Fungus symbiotic fermentation blood sugar-reducing moringa oleifera and preparation method thereof | |
| CN104382129A (en) | Beverage prepared from sweet corn and black tea fungus | |
| CN110423699A (en) | A kind of mycelium liquid fermentation medium composition of selenium-rich agaric and preparation method thereof and fermentation process | |
| CN104273628A (en) | Biological beverage taking coix seed as raw material and preparation of biological beverage | |
| CN109349610A (en) | A kind of compound edible mushroom ferment and preparation method thereof | |
| CN101125019B (en) | Pholiota adiposa mycelium fermentation vinegar beverage and preparation method | |
| CN104286280A (en) | Pawpaw and red tea fungus drink | |
| CN105725163B (en) | A kind of Lenlinus edodes black garlic sauce | |
| CN104287044A (en) | Kiwi fruit and red tea fungus drink | |
| CN104287045A (en) | Large green date and tea fungus beverage | |
| CN101180984B (en) | Edible mythic fungus bacterium ball milk and preparation method thereof | |
| CN113693205A (en) | Healthy flavored soybean milk formula and production process thereof | |
| CN116138315A (en) | Polygonatum sibiricum composite fermentation functional soybean milk and preparation method thereof | |
| CN105614830B (en) | A kind of agaric fungus black garlic sauce | |
| CN104273611A (en) | Tomato tea fungus beverage |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110406 Termination date: 20201212 |