CN101165472B - Multiple-pass mode capillary tube electrophoresis device - Google Patents
Multiple-pass mode capillary tube electrophoresis device Download PDFInfo
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- CN101165472B CN101165472B CN200610048056A CN200610048056A CN101165472B CN 101165472 B CN101165472 B CN 101165472B CN 200610048056 A CN200610048056 A CN 200610048056A CN 200610048056 A CN200610048056 A CN 200610048056A CN 101165472 B CN101165472 B CN 101165472B
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Abstract
The continues laser light outputted by a 532nm laser source is focused on the detection window of the capillary; the capillary array is fixed by using Teflon ring; the fluorescence signals generated by the sampler are collected by the rotary reflector and arrives at the photomultiplier through optical path; a high speed DC motor drives the rotary reflector to make laser scan so as to speed up the data collection; a rotary encoder is used for reading out the location signal of the capillary and triggering the data collection system to make fluorescence collection; a high voltage relay is used for controlling the electrophoresis high voltage and realizing the electromotion sample introduction.
Description
Technical field
The present invention relates to the efficient capillary array electrophoresis system, specifically be based on the multiple-pass mode capillary tube electrophoresis device of 532nm laser-Induced Fluorescence Detection, research fields such as its rapid Optimum for the sample separation condition, combination asymmetric catalysis condition high flux screening provide a kind of reliable analysis means.
Background technology
Development along with combinatorial chemistry, genomics and proteomics is being faced with unprecedented pressure to compound analysis.Sample becomes increasingly complex, and amount of analysis is increasing, and complicated sample requires analysis means preferably, and a large amount of samples then requires higher analysis throughput.High performance capillary electrophoresis (High Performance Capillary Electrophoresis, HPCE) have advantages such as separation efficiency height, analysis speed is fast, sample volume is little, application aspect analytical chemistry is increasingly extensive, has become a kind of important analysis tool.But it can only analyze a kind of sample at every turn, carry out high throughput analysis of samples and many capillaries need be formed arrays, promptly capillary array electrophoresis (Capillary ArrayElectrophoresis, CAE).
Capillary array electrophoresis is because of the Human Genome Project (Human Genome Project, HGP) demand of order-checking quantity jump level is arisen at the historic moment, it is at first proposed in 1992 by Mathies research group, and each R﹠D institution has developed different capillary array electrophoresis devices respectively according to the needs of research subsequently.At present, multiple commercial efficient capillary array electrophoresis instrument has appearred on the market, mainly be divided into capillary gel electrophoresis (CGE) and capillary zone electrophoresis (CZE), on purposes, the former is mainly used in gene sequencing, and the latter is except all occasions that can be used for various single channel high performance capillary electrophoresis and be suitable for, new have more challenging field at some, as proteome analysis, separation and reaction condition optimization, enzyme assay, combinatorial chemical library screening etc., has more superiority.But these commercialization capillary array electrophoresis instrument mainly still are used for dna sequence analysis, and cost is too high.
(Laser-induced Fluorescence LIF) becomes the main detection mode of capillary array electrophoresis to laser-induced fluorescence (LIF) because of detection sensitivity is high, common have two kinds of scan-type and imaging types.Imaging type detects laser intensity is had relatively high expectations, and the laser intensity that shines on the capillary detection window is consistent, because capillary array is tight, occurs the phase mutual interference of fluorescence signal between the adjacent kapillary easily; Scan-type detects the laser intensity requirement lower, but the introducing of rotatable parts causes relatively difficulty of location capillaceous, and deviation appears in acquisition speed slowly and easily.
Chinese patent (application number: 03133511.X) disclose the burnt detector of a kind of capillary array electrophoresis rotary laser scanning copolymerization, be used for separating and detection.This patent adopts gas laser as excitation source, and input mode is single, lack the electrophoresis high voltage system, and gas laser unstable properties, serviceable life are short simultaneously.
Summary of the invention
The object of the present invention is to provide a kind of multiple-pass mode capillary tube electrophoresis device that detects based on the 532nm laser-induction fluorescence co-focusing, can solve the problem of the existence of prior art, have high flux, accurate positioning, flexible, the cheap characteristics of use.
For achieving the above object, the technical solution used in the present invention is:
A kind of multiple-pass mode capillary tube electrophoresis device based on the 532nm laser-Induced Fluorescence Detection, it mainly is made up of semiconductor laser, optical element, high-voltage power supply, high-voltage relay, kapillary, buffer pool, electrode, reometer, twin shaft DC micromotor, absolute rotary encoder, decoding scheme, photomultiplier, data collecting card, computing machine, plate and pillar.
The twin shaft DC micromotor is fixed in the locating slot at plate center, plate is fixed by shore supports, one of twin shaft DC micromotor is connected with absolute rotary encoder by shaft joint, absolute rotary encoder is connected with decoding scheme (signal of absolute rotary encoder output passes to decoding scheme), and decoding scheme is connected with computer circuitry again.
Capillary array adopts the circular symmetry layout, fix by the polytetrafluoroethylene ring that is carved with 1,2,4,8,16,32, the 64 or 128 even grooves of equal portions, two ends capillaceous place buffer pool respectively, the one end is joined by relay and electrophoresis high-voltage power supply by the electrophoresis anode, and the other end is linked to each other together through reometer by the electrophoresis negative electrode.
Another axle of described twin shaft DC micromotor is fixed with the rotor of lower end for " V " font, two planes of " V " font and the angle of surface level are 45 °, wherein a plane is milled with circular groove, first catoptron (500nm-650nm) is fixed in the groove, its reflecting surface is horizontal by 45, and first catoptron places on the central shaft of circular symmetry layout capillary array, first catoptron on the central shaft can carry out relative the rotation with the kapillary of circular symmetry layout, carry out laser scanning, be provided with first lens of horizontal positioned under first catoptron, be fixed with dichroic mirror under first lens horizontal by 45, be placed with second catoptron horizontal by 45 under the dichroic mirror, second catoptron places on the laser optical path of semiconductor laser.
On the reflected light path of dichroic mirror, be provided with long pass filter sheet, second lens and diaphragm successively, passing to photomultiplier through second lens focus to the fluorescence signal on the diaphragm detects, photomultiplier changes light signal into electric signal, after 12 bit data capture card A/D conversion, by Computer Storage.
Described two ends capillaceous place buffer pool respectively, the one end places the sample buffer pond, the other end places shared buffering waste liquid pool, the sample buffer pond is an annular layout, porose on it, be used to place sample and buffer solution centrifuge tube, support by the pole that can carry out highly regulating, and the lower surface of pole can be moved on surface level; The buffering waste liquid pool is an annular layout, is positioned over the middle part in annular sample buffer pond, and first lens place the annular center of buffering waste liquid pool by set collar; The periphery of described set collar is provided with external thread, and the interior ring of buffering waste liquid pool is processed with internal thread, and set collar is threaded with the buffering waste liquid pool, and can pass through its vertical height of threaded adjusting.
The present invention has following advantage:
1. high throughput analysis of samples, separation condition screening fast.The present invention has the eight kinds of channel patterns that can select, is respectively 1,2,4,8,16,32,64,128 passages, is controlled by the fork shift switch above the decoding scheme; The highlyest analyze the screening of separation condition fast when carrying out 128 kinds of samples.
2. use flexible, handling safety, cheap.The present invention can select the capillary channel number according to the research needs, both can adopt gravity siphon sample introduction, also can adopt electrokinetic injection, and input mode is flexible, and high-voltage relay can effectively be controlled electrophoresis high pressure, handling safety, cheap.
3. acquisition speed is fast, accurate positioning, and data are reliable.The present invention adopts the miniature high-speed direct current generator to drive first catoptron to carry out laser scanning, accelerated the speed of data acquisition; The absolute rotary encoder of employing gray encoding obtains position signalling capillaceous and the trigger data acquisition card carries out the fluorescence signal collection to it, has solved orientation problem capillaceous; Simultaneously, laser instrument, optical element, photomultiplier, pillar all are fixed on the vibration isolation precision optical platform, realized the coaxial of rotary encoder, high speed direct current generator, first catoptron, laser beam, the rotor pole the earth of " V " font design has weakened the vibration when direct current generator is worked, and has improved accurate, the reliability of data.
4. the semiconductor laser that adopts exportable continuous laser is as excitation source, and it has, and volume is little, stable performance, life-span are long, cheap, and the characteristics of adjustable power.
5. the buffering waste liquid pool is an annular layout, and is symmetrical with the annular layout in sample buffer pond, reduced the distortion tension force of kapillary fixedly the time, be convenient to capillaceous fixing with operate.
6. plate of the present invention is provided with pilot hole, and whole device adopts the basic hole system location, installs each arrangement of parts compactness, the vibration when having weakened direct current generator work.
In a word, in a kind of multi-channel mode efficient capillary array electrophoresis instrument of the present invention based on the detection of 532nm laser-induction fluorescence co-focusing,, stable performance little with volume, life-span are long, cheap, and the adjustable 532nm semiconductor laser of laser power is as excitation source; Capillary array is fixed by the polytetrafluoroethylene ring that is carved with the even groove of 128 equal portions through precision optical machinery; The high speed direct current generator that rotational speed is adjustable and sense of rotation is variable drives the speed that first catoptron carries out laser scanning and accelerated data acquisition, position signalling capillaceous read by rotary encoder and trigger data acquisition sticks into capable fluorescence signal collection, eight kinds of alternative path patterns have been realized simultaneously, relay control electrophoresis high pressure has also been realized electrokinetic injection, the digital display type reometer is used for the total current of pick-up unit, each spare and accessory parts is arranged compact, vibration when the first catoptron rotor of particular design has weakened the work of high speed direct current generator has improved and can locate number capillaceous and accuracy.The invention solves scan-type detect in acquisition speed reach orientation problem capillaceous slowly, overcome imaging type detect in the shortcoming of fluorescence signal phase mutual interference between the kapillary, improved the reliability of data, have simultaneously accurate positioning, velocity of separation fast, use flexibly, handling safety, cheap advantage, and can select the capillary channel number according to the needs of research, be particularly suitable for research fields such as high throughput analysis of samples, separation and reaction condition optimization and combinatorial chemical library screening.
Description of drawings
Fig. 1 is a 532nm capillary array electrophoresis device synoptic diagram.
Fig. 2 is a 532nm capillary array electrophoresis device core component sectional view.
Fig. 3 is the electrophoretogram of 128 channel separation rhodamine 6Gs.
Fig. 4 is the electrophoretogram of 16 channel separation rhodamine class mixed dyes.
Embodiment
As shown in Figure 1, 2, a kind of multiple-pass mode capillary tube electrophoresis device based on the 532nm laser-Induced Fluorescence Detection, it mainly is made up of semiconductor laser 1, optical element, high-voltage power supply 10, high-voltage relay 11, kapillary 14, buffer pool, electrode 12, reometer 16, twin shaft DC micromotor 19, first catoptron 5, absolute rotary encoder 17, decoding scheme 22, photomultiplier 9, data collecting card 24, computing machine 23, plate 25 and pillar.
Twin shaft DC micromotor 19 is fixed in the center of plate 25, and plate 25 is by pillar 29 support fixation; One of twin shaft DC micromotor 19 is connected with absolute rotary encoder 17 by shaft joint 18, and the signal that absolute rotary encoder 17 is exported passes to decoding scheme 22, and decoding scheme 22 is connected with computing machine 23 circuits again.
The two ends of kapillary 14 arrays place buffer pool respectively, the one end places sample buffer pond 13, the other end places shared buffering waste liquid pool 15, sample buffer pond 13 is an annular layout, support by the pole 28 that can regulate height, the lower surface of pole 28 can be moved on surface level, buffering waste liquid pool 15 is an annular layout, buffering waste liquid pool 15 is positioned over 13 middle parts, annular sample buffer pond, first lens 4 place the annular center of buffering waste liquid pool 15 by set collar 27. and the periphery of set collar 27 is provided with external thread, be processed with internal thread on the interior ring of buffering waste liquid pool 15, set collar 27 is threaded with buffering waste liquid pool 15, and can pass through its vertical height of threaded adjusting.
On the reflected light path of dichroic mirror 3, be provided with long pass filter sheet 6, second lens 7, diaphragm 8 successively, the fluorescence signal that focuses on the diaphragm 8 passes to photomultiplier 9 detections, photomultiplier 9 changes fluorescence signal into electric signal, after 12 bit data capture card 24A/D conversion, by computing machine 23 storages.
Miniature high-speed direct current generator 19 drives first catoptron 5 and carries out laser scanning, and has accelerated the speed of data acquisition; E6CP-AG3C type rotary encoder 17 is read the position signalling of kapillary 14 arrays, and 24 pairs of these kapillaries of trigger data acquisition card carry out the fluorescence signal collection; The 532nm continuous laser of semiconductor laser 1 emission focuses on the detection window of kapillary 14 arrays through second catoptron 2, dichroic mirror 3, first lens 4, first catoptron 5, the fluorescence that sample produces is collected by first catoptron 5, become directional light through first lens 4, dichroic mirror 3 reflections, long pass filter sheet 6 filtering, second lens 7 focus on the diaphragm 8, and pass to photomultiplier 9 and detect, photomultiplier 9 changes fluorescence signal into electric signal, after 12 bit data capture card 24A/D conversion, by computing machine 23 storages.
With the rhodamine 6G is the feasibility that analytic sample has been verified 128 passage capillary array electrophoresis.Adopt internal diameter 50 μ m, the fused quartz kapillary of external diameter 375 μ m, total length is 25cm, effective length is 23cm.Use distilled water that the rhodamine 6G methanol mother liquor is diluted to 1 * 10
-6Mol/L is as analytic sample, and to contain the pH=10 of 5% methyl alcohol (v/v), the 10mmol/L borax soln is a running buffer.With the capillary array of 128 above-mentioned specifications along circular symmetry be fixed on the polytetrafluoroethylene ring 20 that is carved with the even groove of 128 equal portions, the capillary array two ends place the buffer solution pond respectively, anode is connected with the electrophoresis high-voltage power supply by high-voltage relay 11, and negative electrode joins through reometer 16 and ground.Fork shift switch on the decoding scheme 22 is transferred to 128 channel positions, and the number of active lanes in the system control program is made as 128.Behind runtime buffer solution 10min under the 10kV separation voltage, disconnect the electrophoresis high pressure by relay 11, electrokinetic injection 10s carries out electrophoretic separation then, the results are shown in Figure 3.Wherein, X-axis is the transit time of rhodamine 6G, and arrow points is the number of active lanes of capillary array.
Employing can be exported the semiconductor laser 1 of 532nm continuous laser as excitation source, it is characterized by that volume is little, stable performance, life-span be long, cheap, and laser power is adjustable.
45 degree 532nm, second catoptron 2 is fixed on the adjustable precision optical mount, is convenient to the adjusting of light path, makes laser beam coaxial with first catoptron 5, has improved the reappearance of device.
Dichroic mirror (see through below the 550nm, 550nm above reflection) 3 is positioned at second catoptron, 2 tops, and with horizontal plane angle be 45 °, its role is to transmission 532nm laser, reflect the fluorescence that sample produces simultaneously and pass to photomultiplier 9 and detect.
Miniature high-speed direct current generator 19 is fixed in the locating slot above the plate 25, and its rotational speed can be regulated, and sense of rotation can change, and have two turning axles, last axle connects absolute rotary encoder 17 by shaft joint 18, and lower shaft connects rotor 21, external power supply.The pillar 29 that plate 25 is 40mm by four diameters is fixing.
Absolute rotary encoder 17 is fixed by the base locating slot, is positioned at the top of direct current generator 19, and with its rotation.Rotary encoder 17 has eight signal wires, eight kinds of different signals of output during work, and each signal has eight kinds of channel patterns corresponding to a kind of channel pattern, and minimum is single channel, is up to 128 passages.Rotary encoder 17 is read the position signalling of kapillary 14 arrays, and its position signalling passes to computing machine 23 through decoding scheme 22, data collecting card 24, and 24 pairs of these kapillaries of trigger data acquisition card carry out the fluorescence signal collection simultaneously.Decoding scheme 22 is provided with the fork shift switch, can select eight kinds of different channel patterns.This design is characterised in that and can selects different channel patterns according to the needs of research that rotary encoder 17 adopts gray encoding simultaneously, can read the position signalling of kapillary 14 arrays more exactly.
Photomultiplier 9 external negative high voltage direct supplys, being used for fluorescence signal detects, the fluorescence that analytic sample produces is collected by first catoptron 5, becoming directional light, dichroic mirror 3 reflections, long pass filter sheet 6 filtering, second lens 7 through first lens 4 focuses on the aperture of diaphragm 8, diaphragm 8 is fixed on the photomultiplier 9, be provided with rotatable light barrier before it, photomultiplier 9 is converted into electric signal with fluorescence signal and passes to capture card 24, and data collecting card 24 is given computing machine after changing it into digital signal.
The rounded symmetrical distribution of kapillary 14 arrays, two ends place buffer pool respectively, and are connected with electrode.In order to eliminate distortion tension force capillaceous, be convenient to capillaceous fixing, sample cell pallet 13 is designed to circle, which is provided with circular port, is used to place sample.The shared waste liquid tank 15 of kapillary 14 arrays, its dark 40mm is positioned in the circular trough in sample buffer pond 13.Sample buffer pond 13 is fixing by three pillars 28, and can regulate.First lens 4 are fixing by set collar 27, are positioned in the circular port of waste liquid pool 15, and vertically adjustable.The light path adjusting is convenient in this design, can realize gravity siphon sample introduction simultaneously.
Electrophoresis high-voltage power supply 10 links to each other with sample cell electrode 12 by relay 11, and the waste liquid pool electrode is connected with ground through digital display type reometer 16.This design is characterised in that and can carries out high voltage control effectively, has improved the security of device, has realized electrokinetic injection, and the electric current that shows by reometer 16 can judge whether electrophoresis buffer solution changes simultaneously.
Laser instrument, optical element, photomultiplier, pillar all are fixed on the vibration isolation precision optical platform 30, realized the coaxial of rotary encoder, high speed direct current generator, first catoptron, laser beam, greatly weaken the vibration when direct current generator is worked, improved accurate, the reliability of data.
Adopt runtime buffer solution with rhodamine 6G, the mixture diluted of rhodamine B is to analytical concentration, with pH=9.0, the 10mmol/L borax soln is as runtime buffer solution. rounded being fixed on symmetrically on the polytetrafluoroethylene ring 20 of 16 capillaries (specification is identical with embodiment 1) array, all insert in the buffer pool at two ends. the fork shift switch on the decoding scheme 22 is transferred to 16 channel positions, number of active lanes in the system control program is made as 16. behind separation voltage 13.0kV runtime buffer solution 10min, disconnect the electrophoresis high pressure by high-voltage relay 11, electrokinetic injection 6s under 5.0kV voltage, under separation voltage, carry out 16 channel array electrophoretic separation then, experimental result is seen Fig. 4. wherein, X-axis is the transit time of sample, arrow points is the capillary channel number, and the sample peak of each passage is followed successively by rhodamine 6G, rhodamine B (from left to right).
Claims (3)
1. multiple-pass mode capillary tube electrophoresis device, it mainly is made up of 532nm semiconductor laser (1), optical element, high-voltage power supply (10), high-voltage relay (11), kapillary (14), buffer pool, electrode (12), reometer (16), twin shaft DC micromotor (19), absolute rotary encoder (17), decoding scheme (22), photomultiplier (9), data collecting card (24), computing machine (23), plate (25) and pillar;
Described optical element comprises dichroic mirror, long pass filter sheet, first lens, second lens, diaphragm, first catoptron and second catoptron;
Kapillary (14) array adopts the circular symmetry layout, fixing by the polytetrafluoroethylene ring that is carved with the even groove of 128 equal portions (20), the two ends of described kapillary (14) array place buffer pool respectively, the one end is joined by high-voltage relay (11) and electrophoresis high-voltage power supply (10) by the electrophoresis anode, and the other end is linked to each other together through reometer (16) by the electrophoresis negative electrode;
Twin shaft DC micromotor (19) is fixed in the locating slot at plate (25) center, plate (25) is by pillar (29) support fixation, one of described twin shaft DC micromotor (19) is connected with absolute rotary encoder (17) by shaft joint (18), absolute rotary encoder (17) is connected with decoding scheme (22) by circuit, and decoding scheme (22) carries out circuit with computing machine (23) again and is connected;
Another axle of described twin shaft DC micromotor (19) is fixed with the rotor (21) of lower surface for " V " font, two planes of " V " font and the angle of surface level are 45 °, wherein a plane is milled with circular groove, first catoptron (5) is fixed in the groove, its reflecting surface is horizontal by 45, and first catoptron (5) places on the central axis of rounded symmetric configuration kapillary (14) array, be positioned on the central axis first catoptron (5) can with relative rotation of kapillary (14) array of circular symmetry layout, carry out laser scanning, be provided with first lens (4) of horizontal positioned under first catoptron (5), be fixed with dichroic mirror (3) under first lens (4) horizontal by 45, horizontal by second catoptron (2) of 45 ° of angles be positioned at dichroic mirror (3) under, and place on the light path of semiconductor laser (1);
On the reflected light path of dichroic mirror (3), be provided with long pass filter sheet (6), second lens (7), diaphragm (8) successively, the fluorescence signal that is focused on the diaphragm (8) by second lens (7) passes to photomultiplier (9) detection, photomultiplier (9) changes fluorescence signal into electric signal, after data collecting card (24) A/D conversion, store by computing machine (23).
2. according to the described multiple-pass mode capillary tube electrophoresis device of claim 1, it is characterized in that: the two ends of described kapillary (14) array place buffer pool respectively, the one end places sample buffer pond (13), and the other end places shared buffering waste liquid pool (15); Sample buffer pond (13) is an annular layout, supports by the pole (28) that can carry out highly regulating, and the lower surface of pole (28) can be moved on surface level; Buffering waste liquid pool (15) is an annular layout, and buffering waste liquid pool (15) is positioned over middle part, annular sample buffer pond (13), and first lens (4) are positioned over the annular center of buffering waste liquid pool (15) by set collar (27).
3. according to the described multiple-pass mode capillary tube electrophoresis device of claim 2, it is characterized in that: the periphery of described set collar (27) is provided with external thread, be processed with internal thread on the interior ring of buffering waste liquid pool (15), set collar (27) and buffering waste liquid pool (15) be through being threaded, and can pass through its vertical height of threaded adjusting.
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| CN101334378B (en) * | 2008-07-29 | 2012-05-16 | 清华大学 | Portable capillary pipe electrophoresis apparatus |
| CN101750450B (en) * | 2008-12-17 | 2013-03-27 | 中国科学院大连化学物理研究所 | Automatic sampling device for array capillary electrophoresis |
| CN101526491B (en) * | 2009-04-16 | 2012-03-21 | 福州大学 | Multi-channel gate of electrochemistry electrode array chip |
| CN102331415B (en) * | 2011-06-15 | 2014-06-04 | 公安部第一研究所 | Method for positioning capillary tube array by using raman spectral imaging |
| CN103234950B (en) * | 2013-05-13 | 2015-01-21 | 上海通微分析技术有限公司 | Parallel dual optical path laser-induced fluorescence spectrograph |
| CN104297155B (en) * | 2014-09-28 | 2016-11-23 | 中国科学院长春光学精密机械与物理研究所 | A kind of multi-channel parallel spectrum investigating system |
| EP3209410A4 (en) * | 2014-10-22 | 2018-05-02 | IntegenX Inc. | Systems and methods for sample preparation, processing and analysis |
| CN105092680B (en) * | 2015-08-18 | 2018-03-27 | 宁波海尔施基因科技有限公司 | Cartridge device applied to HPCE |
| CN106018403B (en) * | 2016-05-12 | 2019-05-21 | 南京擎科生物科技有限公司 | The light absorption detector and detection method of capillary array electrophoresis instrument |
| CN105973860B (en) * | 2016-06-29 | 2019-07-30 | 山东科立森生物股份有限公司 | A kind of analysis system of capillary electrophoresis and detection method |
| CN109690306B (en) * | 2016-09-09 | 2023-08-01 | 生命技术公司 | Capillary electrophoresis cathode system and method |
| EP3401665A1 (en) * | 2017-05-12 | 2018-11-14 | University College Dublin National University Of Ireland, Dublin | A system and device for analysis of specific matter in liquid samples by optical microscopy |
| CN108593554B (en) * | 2018-05-24 | 2020-10-30 | 大连民族大学 | Laser-induced fluorescence collection and photoelectric conversion device capable of being adjusted in multiple dimensions |
| CN108627489B (en) * | 2018-05-24 | 2021-04-13 | 大连民族大学 | 128-channel array capillary electrophoresis apparatus |
| CN113252763A (en) * | 2021-04-21 | 2021-08-13 | 上海应用技术大学 | Multi-channel automatic sample introduction device for capillary electrophoresis fluorescence analysis |
| WO2022257944A1 (en) * | 2021-06-08 | 2022-12-15 | 南京金斯瑞生物科技有限公司 | Optical element detachable capillary clip and capillary electrophoresis instrument |
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| US20030150727A1 (en) * | 1999-01-27 | 2003-08-14 | Affymetrix, Inc. | Monster capillary array electrophoresis scanner |
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