CN101165469A

CN101165469A - Protein coated silver sulfide nano line preparation method

Info

Publication number: CN101165469A
Application number: CNA200610150298XA
Authority: CN
Inventors: 杨林; 刘山虎; 邢瑞敏; 张保芳
Original assignee: Henan Normal University
Current assignee: Henan Normal University
Priority date: 2006-10-19
Filing date: 2006-10-19
Publication date: 2008-04-23
Anticipated expiration: 2026-10-19
Also published as: CN101165469B

Abstract

The method features the following: the argentic sulfide nanometer lines covered by the protein are made of bovine serum albumin (BSA) and thioacetamide (TAA). After the chelation between the metal ion and the biomacromolecule, the chelate of silver ion is formed; said chelate and TAA releases sulfur ions which are nucleated in the multi-hole alumina shuttering so as to gradually form the nanometer lines; after separation and cleaning, the argentic sulfide nanometer lines covered by the protein can be obtained.

Description

The preparation method of the silver sulfide nano line that a kind of protein coats

Technical field

The present invention relates to the function nano technical field of material, promptly designed a kind of preparation method of silver sulfide nano line of biomacromolecule bovine serum albumin(BSA) coating, made the nano material of similar " cable " structure.

Background technology

Silver sulfide, copper sulfide, cadmium sulfide, cadmium selenide, cadmium telluride etc. are not only good semiconductor material, and have optical characteristics such as visible absorption, main infrared light see through, photoluminescence.Especially when its size reaches nanometer, optical physics and spectrochemical property all have greatly improved, have good spectral characteristic and photochemical stability, therefore it has potential application prospect as biological fluorescent labeling at biomedical aspect, and the research of the semiconductor nano material that biological surface is modified is to developing new luminescent material and the novel fluorescence probe is significant.

The preparation method of at present relevant nano wire is existing a lot, be divided into physical vaporous deposition, direct current deposition method and thermal evaporation etc. substantially, above method respectively has characteristics of oneself and suitable system, as physical vaporous deposition technical equipment is had relatively high expectations, thermal evaporation temperature height, direct current deposition method is comparatively complicated, and for the preparation of protein coating system certain limitation is arranged.Therefore explore that a kind of temperature of reaction is not high, pressure is little, energy consumption is low, technological process is simple, be fit to the preparation method of the nano wire that bioprotein modifies, biomedicine is had very high practical significance.

Summary of the invention

Similar " cable " structure nano line technology that the object of the present invention is to provide a kind of reaction conditions gentleness, the simple synthetic protein of instrument and equipment to coat.This preparation facilities comprises the container slot that is separated by porous alumina membrane, one container slot fills the silver ion of protein chelating, an other container slot is equipped with provides the TAA of sulphion solution, this experiment was divided into for two steps: at first be that protein and silver ion chelating form chelate, chelate and sulphion form the silver sulfide nano line that protein coats by diffusion reaction in the porous alumina membrane duct then.

The silver sulfide nano line preparation method's that protein coats concrete steps are as follows:

1. protein and silver ion stirred chelating 20 minutes;

2. chelate solution and thioacetyl amine aqueous solution are poured into respectively in the container slot at disperser two ends;

3. spread 72 hours under the room temperature;

4. take out template and dissolve template with sodium hydroxide solution;

5. centrifuging, redistilled water clean, vacuum drying obtains the silver sulfide nano line that protein coats;

The useful effect that the present invention has is:

1. reaction conditions is to carry out in normal temperature, normal pressure and the aqueous solution, mild condition, environmental friendliness;

2. reaction unit and technical operation step are simple;

3. the technological gap of biomacromolecule clad nano line has been filled up in this invention, is other biomacromolecule table simultaneously

The preparation of the nano-substance that face is modified provides experimental program and foundation;

4. the diameter of product and length can be controlled according to actual needs;

5. the semiconductor nanowires of this biomacromolecule coating has broad application prospects in fields such as biological fluorescent labeling, microelectrode, bio-sensing and drug conveying;

In a word, the present invention has the nano material of change biocompatibility, energy consumption is low, reduces reaction cost, improves advantages such as reaction safety.By changing reaction conditions, can regulate size and pattern that control protein coats silver sulfide nano line, and temperature of reaction is low can not destroy bioprotein.

Description of drawings

Fig. 1 is transmission electron microscope (TEM) picture of the synthetic sample of the invention process example 1, and scale is 50nm among the figure, and nanowire diameter is 50nm;

Fig. 2 is transmission electron microscope (TEM) picture of the silver sulfide nano line that coats of the synthetic protein of the invention process example 2, and scale is 100nm among the figure, and nanowire diameter is 50nm;

Fig. 3 is transmission electron microscope (TEM) picture of the silver sulfide nano line that coats of the synthetic protein of the invention process example 3, and scale is 50nm among the figure, and nanowire diameter is 50nm;

Fig. 4 is scanning electron microscope (SEM) picture of the silver sulfide nano line that coats of the synthetic protein of the invention process example 3, and scale is 1 μ m among the figure, and nanowire diameter is 50nm;

Fig. 5 is transmission electron microscope (TEM) picture of the silver sulfide nano line that coats of the synthetic protein of the invention process example 3, and scale is 0.5 μ m among the figure, and nanowire diameter is 50nm;

Fig. 6 is transmission electron microscope (TEM) picture of silver sulfide nano line after special processing that the invention process example 3 synthetic protein coat, and scale is 50nm among the figure, and nanowire diameter is 50nm;

Fig. 7 is the X-diffracting spectrum of the silver sulfide nano line that coats of the synthetic protein of the invention process example 3;

Fig. 8 is the chemical element energy spectrum analysis (EDX) of the silver sulfide nano line that coats of the synthetic protein of the invention process example 3;

Embodiment

Examples of implementation 1:

(1) bovine serum albumin solution and liquor argenti nitratis ophthalmicus reacted about 20 minutes under electromagnetic agitation, obtained chelate solution;

(2) above-mentioned chelate solution and thioacetyl amine aqueous solution are poured into respectively in two container slot that separated by porous alumina membrane;

(3) spread 12 hours under the room temperature;

(4) taking out porous alumina membrane is this film of dissolution of sodium hydroxide of 2M with concentration also;

(5) high speed centrifugation separation, redistilled water clean, three times like this, last room temperature vacuum drying sample, TEM (transmission electron microscope) analysis shows, find that reaction product is the graininess silver sulfide nano particle that is wrapped in the protein matrix, is not combined into wire (as Fig. 1) as yet in these examples of implementation;

Examples of implementation 2: experimental procedure (1) (2) (4) (5) is with examples of implementation 1, step (3) makes reaction spread 48h, last room temperature vacuum drying sample, it is carried out TEM (transmission electron microscope) analysis, find that reaction product has begun reunion by nano particle and has been combined into wire, but combination not very tight (as Fig. 2);

Examples of implementation 3: experimental procedure (1) (2) (4) (5) is with examples of implementation 1, and step (3) makes reaction spread 72h, and last room temperature vacuum drying sample carries out TEM (transmission electron microscope) analysis to it, obtains the silver sulfide nano line (as Fig. 3) that protein coats.From Fig. 3,4,5 as can be seen, reaction product has been assembled into the nano wire that complete skin coats protein in these examples of implementation; Fig. 3 shows that sample diameter approximately is 50nm; Fig. 4 is the sample sem photograph, shows that sample dispersion is better in these examples of implementation; Fig. 5 is the transmission electron microscope picture of sample, show sample length in micron dimension, and homogeneity is better; Coated by light grey protein around Fig. 6 can obviously find out silver sulfide nano line, sample diameter approximately is 50nm as can be seen simultaneously; Fig. 7 is the XRD diffracting spectrum of sample, proves that sample is a monocline silver sulfide crystal; Fig. 8 is the element energy spectrogram (EDX) of sample, the analysis showed that Ag and the atom number ratio of S are 12.1%: 6.2%, approximates 2: 1, and the composition that further proves sample is a silver sulfide.

Claims

1. the synthetic method of the silver sulfide nano line that coats of a protein, it is characterized in that: at normal temperatures and pressures, metallic ion and biomacromolecule form chelate solution in the aqueous solution, chelating ion and sulphion are located nucleation in porous alumina formwork then, be assembled into nano wire gradually, promptly get the silver sulfide nano line that protein coats after the separation.

2. the preparation method of the silver sulfide nano line that coats according to the described protein of claim 1, its feature can be silver, gold, copper, cadmium, chromium, zinc, mercury, plumbous plasma at the reacting metal ion; Negative ion can be sulphur, selenium and tellurium plasma.

3. the preparation method of the silver sulfide nano line that coats according to the described protein of claim 1 is characterized in that the reaction conditions temperature is about 10-50 ℃; Pressure is normal pressure.

4. the preparation method of the silver sulfide nano line that coats according to the described protein of claim 1 is characterized in that described protein can select bovine serum albumin(BSA), bovine hemoglobin, human serum albumins, human hemoglobin and sheep blood serum albumin for use.

5. the preparation method of the silver sulfide nano line that coats according to the described protein of claim 1 is characterized in that reactant concentration protein is 2%, and metallic ion is 50mmol/L, and negative ion is 50mmol/L.

6. the preparation method of the silver sulfide nano line that coats according to the described protein of claim 1, it is characterized in that reactant adding method is, solution with metal ion under lasting the stirring slowly joins protein solution, stirs after 20 minutes, pours this solution into disperser again.

7. the preparation method of the silver sulfide nano line that coats according to the described protein of claim 1 is characterized in that the reaction time is about 72 hours.

8. the preparation method of the silver sulfide nano line that coats according to the described protein of claim 1, it is characterized in that the separated and dissolved process, after using 2M sodium hydroxide solution dissolving template, high speed centrifugation separates, use the second distillation water washing then, so triplicate centrifuge washing process gets product after the room temperature vacuum drying.