CN101156643A - A technics for preparing hydrolytic collagen albumen taking twilight colours jellyfish as raw material - Google Patents
A technics for preparing hydrolytic collagen albumen taking twilight colours jellyfish as raw material Download PDFInfo
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- CN101156643A CN101156643A CNA2007100411282A CN200710041128A CN101156643A CN 101156643 A CN101156643 A CN 101156643A CN A2007100411282 A CNA2007100411282 A CN A2007100411282A CN 200710041128 A CN200710041128 A CN 200710041128A CN 101156643 A CN101156643 A CN 101156643A
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Abstract
The invention discloses a process for preparing hydrolyzed collagen protein using jellyfishes as the raw material, and belongs to the natural resource processing and utilizing technical field. The invention uses a novel natural marine biological resource, i.e. jellyfishes, as the raw material for preparing hydrolyzed collagen protein, and realizes the conversion of the role of the jellyfishes from a marine ecological disaster species to a novel natural marine biological collagen protein resource. By operating according to the invention, 2 percent of hydrolyzed collagen protein with more than 95 percent of purity, less than 3000 molecular weight and more than 85 percent of peptides can be obtained. The hydrolyzed collagen protein is known as an important healthy food and cosmetic raw material, and has wide range of application in the clinical medicine, the textile industry and the fine chemical industry, etc. Developing and utilizing the jellyfishes used as the natural marine biological resource has great significance to the fast growing of the jellyfishes, to the effective protection of marine ecologic environment of China and to the rapid recovery of the marine fishery.
Description
Technical field
It is the technology of feedstock production Hydrolyzed Collagen with the rosy clouds jellyfish that this patent relates to a kind of, belongs to the process and utilization technology field of natural resources.
Background technology
Rosy clouds jellyfish (Cyanea) is commonly called as fiber crops and bites, and is a kind of large ocean planktonic organism, belong to Cnidaria (Phylum Cnidaria), Scyphozoa (ClassScyphomedusae), Semaeostomeae (Order Phylum cnidaria), rosy clouds jellyfish section (Family Cyanea).The coastal adularescent rosy clouds jellyfish of having found of China (Cyanea nozaki Kishinouye), a shape rosy clouds jellyfish (Cyanea capillata), brown rosy clouds jellyfish (Cyanea ferrugineaEschscholtz), with 4 kinds of purple rosy clouds jellyfish (Cyanea purpurea Kishinouye), wherein with white rosy clouds jellyfish quantity at most, distribution is the widest.The rosy clouds jellyfish is a bait with small-sized zooplankter usually, its gonad prosperity, and fertility is strong, and the speed of growth is exceedingly fast, and the annual production of China rosy clouds jellyfish has at present constituted a kind of new natural marine organism collagen resource up to ten million ton.
Hydrolyzed Collagen is a kind of novel collagen series products, is to be that raw material is produced with collagen or the resource that is rich in collagen.(about 30% of total amino acid residue is glycine except the feature amino acid with collagen is formed for it, other has two seed amino acids seldom of content in other protein of 15-30%, proline and 4-Hydroxyproline) outside, it is less also to have molecular weight, is lower than the characteristics of 3000 little peptide in the constituent based on molecular weight.This makes that Hydrolyzed Collagen is easier to directly be absorbed by corium through epidermis, is easier to be digested and assimilated by alimentary canal, has purposes widely in fields such as skin care item, cosmetic health products, is subjected to consumer's welcome deeply.
About the preparation of Hydrolyzed Collagen, the collagen that domestic and international research is more common in sources such as the skin of the skin of pig, ox, bone, rib and fish, bone, squamas is on the preparation method and technology of raw material.With the rosy clouds jellyfish is that any report is not seen in the research of feedstock production Hydrolyzed Collagen both at home and abroad as yet.
Summary of the invention
It is the process route of feedstock production Hydrolyzed Collagen with the rosy clouds jellyfish that this patent has been invented one.Comparatively unique is, it has realized the purpose that the rosy clouds jellyfish is developed and utilized with a kind of novel natural marine organism resource-Xia jellyfish (Cyanea)-process as the preparation raw material of Hydrolyzed Collagen.Operate according to this patent, can obtain the Hydrolyzed Collagen product with high productive rate.This is vital for the comprehensive utilization of this natural marine organism resource of rosy clouds jellyfish.
Hydrolyzed Collagen is known as important health food and cosmetic material, in addition, also has purposes widely at aspects such as clinical medicine, textile industry and fine chemistry industries.
Specifically, at first, we find that the commercially available fiber crops of pickling processing through three alum bite the preparation raw material that can be used as Hydrolyzed Collagen after the process desalting processing.It is stable that wherein contained collagen is handled the water logging bubble, under the soaking technology condition that we taked, neither can be degraded, be extracted in the water and run off because of being subjected to soaking, can not influence its dispersiveness in follow-up hydrolysis process and the functional characteristic of obtained aqueous solution because of being subjected to soak yet, promptly the commercially available fiber crops of pickling processing through three alum bite with the fresh rosy clouds jellyfish of just having fished for disembarkation and have equal machinability, all can be used as the raw material for preparing Hydrolyzed Collagen.Established thus with the rosy clouds jellyfish is the basis of raw material processing and preparing Hydrolyzed Collagen.
Secondly, after the investigation and contrast of system carried out in various collagen hydrolysate agent commonly used such as hydrolysising condition such as temperature, pressure and NaOH, hydrochloric acid, Collagenase, we find, under suitable hydrolysising condition, the collagen that is contained in the rosy clouds jellyfish just can directly be hydrolyzed without degenerative treatments, is distributed in the water, obtain clear, impurities hydrolysis dispersion liquid seldom.
We find that in soaking hydrolysis process parameters such as solvent, soak time, hydrolytic reagent, the temperature that is adopted when the kind of hydrolytic reagent and hydrolysis is of paramount importance for the hydrolysis yield.Wherein, effectively hydrolytic reagent is a kind of in common alkali (as hydroxide or carbonate, the ammoniacal liquor etc. of sodium, potassium, calcium, barium), common acid (organic acids such as inorganic acid such as example hydrochloric acid, phosphoric acid, sulfuric acid or formic acid, acetic acid, malic acid, lactic acid, citric acid) or the various protease (as pepsin, trypsase, pancreas chymotrypsin, acid protease, alkali protease, neutral proteinase, papain, bromelain, Collagenase, elastoser etc.) or some kinds.When selecting for use NaOH to be hydrolytic reagent, the pH value should be within the 7.0-14.0 scope, and when selecting for use hydrochloric acid to be hydrolytic reagent, the pH value should be within the 0.5-6.8 scope, and when selecting for use protease to be hydrolytic reagent, the pH value should be within the optimal pH scope of selected enzyme; When selecting for use soda acid to be hydrolytic reagent, hydrolysis temperature should preferably in 0-60 ℃ scope, when selecting for use protease to be hydrolytic reagent, should be hydrolyzed under the optimum temperature of protease in-30-200 ℃ scope.Other parameters are soaked desalination and need be carried out 6-15 time, and soak required water consumption at every turn and be the rosy clouds jellyfish raw material thrown in 1-5 times, soaking temperature is in-50-98 ℃ scope, and soak time is 0.5-12hr time
-1The drying mode of liquid can be freeze drying, also can be spray-drying.
The specific embodiment
Get 1000g three alum and pickle the commercially available fiber crops of processing and bite, behind the draining, be immersed in the deionized water that 2-5 doubly measures and soak, and changed one time water in every 2-3 hour, till the content of sodium chloride is lower than 1.5ppm in soaking water (chlorion detects and is negative).Desalination rosy clouds jellyfish after the immersion is regulated pH to 5.8-6.8 (a kind of optimal pH of specific complex Collagenase), be incubated digestion 12-16 hour down 38-40 ℃ (optimum temperature of this Collagenase), obtain the liquid of a clear, be Hydrolyzed Collagen liquid; Hydrolyzed Collagen liquid cooling freeze-drying is dry, obtains 20g purity and reaches more than 95%, and molecular weight accounts for Hydrolyzed Collagen powder solid more than 85% in the peptide class below 3000.
Claims (7)
1. one kind is the technology of feedstock production Hydrolyzed Collagen with the rosy clouds jellyfish.This technology is made up of the following step:
(1) is that solvent soaks the rosy clouds jellyfish with water, removes the salinity that is contained in the raw material rosy clouds jellyfish.
(2) to the rosy clouds jellyfish that the has removed salinity processing that is hydrolyzed, the rosy clouds jellyfish is melted, obtain a colourless or flaxen liquid.
(3) resulting liquid, dry then through (or without) homogeneous, bleaching, desalting processing, obtain Hydrolyzed Collagen.
The rosy clouds jellyfish of being declared, be commonly called as fiber crops and bite, mean fresh product or its curing food of pickling through three alum of fishing for of rosy clouds jellyfish section marine organisms white rosy clouds jellyfishes (Cyanea nozaki Kishinouye), shape rosy clouds jellyfish (Cyanea capillata), brown rosy clouds jellyfish (Cyaneaferruginea Eschscholtz) or purple rosy clouds jellyfish (Cyaneapurpurea Kishinouye).
The hydrolysis process of being declared means under certain temperature, pressure, with specific hydrolytic reagent carries out the rosy clouds jellyfish is melted in the water, forms the process of liquid.
The drying of being declared obtains the process of solid for making liquid removal moisture.
The Hydrolyzed Collagen of being declared is the hydrolysate of contained collagen in the rosy clouds jellyfish.Its protein content 〉=95%, shared ratio 〉=85% of Hydrolyzed Collagen peptide class (molecular weight≤3000) in the albumen.
2. the water system of declaring in the claim 1 refers to that pH is adjusted to the running water or the deionized water of certain certain value, and its pH value is within the 2-12 scope.
3. the immersion of declaring in the claim 1 is carried out 6-15 time, the 1-5 that soaks required water consumption at every turn and be the rosy clouds jellyfish raw material of being thrown in doubly, soaking temperature is in-50-98 ℃ scope, soak time is 0.5-12hr time
-1
4. the hydrolysis process of declaring in the claim 1, its operating pressure is 0.0001-0.5MPa.
5. the hydrolysis process of declaring in the claim 1, its operating temperature be-30-200 ℃.
6. the hydrolysis process of declaring in the claim 1, the hydrolytic reagent that can Gong select for use is 0.1～10molL
-1Alkali (as hydroxide or carbonate, the ammoniacal liquor etc. of sodium, potassium, calcium, barium), 0.1～10molL
-1Acid (organic acid such as inorganic acid such as example hydrochloric acid, phosphoric acid, sulfuric acid or formic acid, acetic acid, malic acid, lactic acid, citric acid) or various protease (as pepsin, trypsase, pancreas chymotrypsin, acid protease, alkali protease, neutral proteinase, papain, bromelain, Collagenase, elastoser etc.) in a kind of or some kinds.
7. the drying of declaring in the claim 1 can be freeze drying, also can be spray-drying.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2007100411282A CN101156643A (en) | 2007-05-24 | 2007-05-24 | A technics for preparing hydrolytic collagen albumen taking twilight colours jellyfish as raw material |
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| CNA2007100411282A CN101156643A (en) | 2007-05-24 | 2007-05-24 | A technics for preparing hydrolytic collagen albumen taking twilight colours jellyfish as raw material |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009140833A1 (en) * | 2008-05-20 | 2009-11-26 | 张丽丽 | Collagen peptide having immunoenchancing activity from cyanea nozakii and preparation method and uses thereof |
| CN101302251B (en) * | 2008-05-20 | 2011-04-20 | 张丽丽 | Cyanea-originated glucoprotein having immunopotentiation activity, preparation and use thereof |
| CN101422266B (en) * | 2008-10-28 | 2011-10-26 | 山东好当家海洋发展股份有限公司 | Jelly-fish nutrient liquid and preparation method thereof |
| CN104334572A (en) * | 2012-07-02 | 2015-02-04 | 诺维信公司 | Polypeptides having xylanase activity and polynucleotides encoding same |
| CN104379593A (en) * | 2012-08-23 | 2015-02-25 | 株式会社海月研究所 | Method for fractionally extracting mucin and collagen |
| CN104940121A (en) * | 2015-07-08 | 2015-09-30 | 广州丽丰化妆品制造有限公司 | Chlamydomonus nivalis cream and preparing method thereof |
| CN110028575A (en) * | 2019-04-18 | 2019-07-19 | 成都中医药大学 | A kind of preparation method of micromolecular collagen |
| CN111393521A (en) * | 2020-03-20 | 2020-07-10 | 张培华 | Extraction method of jellyfish collagen |
| CN111437242A (en) * | 2020-05-07 | 2020-07-24 | 江苏华能药业有限公司 | Crystal mask prepared from jellyfish |
-
2007
- 2007-05-24 CN CNA2007100411282A patent/CN101156643A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009140833A1 (en) * | 2008-05-20 | 2009-11-26 | 张丽丽 | Collagen peptide having immunoenchancing activity from cyanea nozakii and preparation method and uses thereof |
| US20110077382A1 (en) * | 2008-05-20 | 2011-03-31 | Lili Zhang | Collagen Peptide Having Immune-enhancing Activity from Cyanea Nozakii and Preparation Method and Uses Thereof |
| CN101302251B (en) * | 2008-05-20 | 2011-04-20 | 张丽丽 | Cyanea-originated glucoprotein having immunopotentiation activity, preparation and use thereof |
| JP2011520927A (en) * | 2008-05-20 | 2011-07-21 | ▲張▼▲麗▼▲麗▼ | Collagen peptide with immunity-enhancing activity derived from the yellow jellyfish and its preparation and use |
| US8450100B2 (en) * | 2008-05-20 | 2013-05-28 | Lili Zhang | Collagen peptide having immune-enhancing activity from Cyanea nozakii and preparation method and uses thereof |
| CN101422266B (en) * | 2008-10-28 | 2011-10-26 | 山东好当家海洋发展股份有限公司 | Jelly-fish nutrient liquid and preparation method thereof |
| CN104334572A (en) * | 2012-07-02 | 2015-02-04 | 诺维信公司 | Polypeptides having xylanase activity and polynucleotides encoding same |
| CN104379593A (en) * | 2012-08-23 | 2015-02-25 | 株式会社海月研究所 | Method for fractionally extracting mucin and collagen |
| CN104379593B (en) * | 2012-08-23 | 2018-01-23 | 株式会社海月研究所 | The fractional extraction method of mucoprotein and collagen |
| CN104940121A (en) * | 2015-07-08 | 2015-09-30 | 广州丽丰化妆品制造有限公司 | Chlamydomonus nivalis cream and preparing method thereof |
| CN110028575A (en) * | 2019-04-18 | 2019-07-19 | 成都中医药大学 | A kind of preparation method of micromolecular collagen |
| CN110028575B (en) * | 2019-04-18 | 2022-12-30 | 成都中医药大学 | Preparation method of micromolecular collagen |
| CN111393521A (en) * | 2020-03-20 | 2020-07-10 | 张培华 | Extraction method of jellyfish collagen |
| CN111437242A (en) * | 2020-05-07 | 2020-07-24 | 江苏华能药业有限公司 | Crystal mask prepared from jellyfish |
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Open date: 20080409 |