CN101144791A - Ion selective solid plane gold electrode sensing chip and its production method - Google Patents
Ion selective solid plane gold electrode sensing chip and its production method Download PDFInfo
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- CN101144791A CN101144791A CNA2007101318986A CN200710131898A CN101144791A CN 101144791 A CN101144791 A CN 101144791A CN A2007101318986 A CNA2007101318986 A CN A2007101318986A CN 200710131898 A CN200710131898 A CN 200710131898A CN 101144791 A CN101144791 A CN 101144791A
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Abstract
The invention relates to an ion selective solid plane gold electrode biology sensing chip and the manufacture method. The invention belongs to the medical electrolyte ion rapid detection technical field. The gold electrode biology sensing chip comprises a substrate and a nanometer rank gold membrane stuck on the substrate, forming an ion selective membrane electrode, a reference electrode and an auxiliary electrode. Plastic piece membranes are covered on the working electrode, the reference electrode and the auxiliary electrode, and form a siphon operating area. The reference electrode is made up of Ag/AgCl. The auxiliary electrode is the gold membrane itself. The liquid tested enters into a plane crevice siphon channel though a siphon, and are accomplished through the working electrode, the reference electrode and the auxiliary electrode. The invention has the advantages that the production cost is low, and the invention is suitable for an ultra large scale production through being combined with the modern process and the classical traditional mature process.
Description
Technical field
The present invention relates to a kind of biological electrolyte sensing chip, belong to medical electrolyte ion rapid detection technical field, also be applicable to food hygiene environmental protection rapid detection technical field.Specifically be a kind of ion selective solid plane gold electrode bio-sensing chip and manufacture method
Background technology
Ion-selective electrode (ion selective electrode) is to be sensor information with solid-state or liquid sensitive membrane, certain ion in the solution is produced optionally response, thereby optionally discern this ion, measures the concentration (activity) of this ion.The electromotive force of ion-selective electrode is relevant with the concentration of ion, and concentration is big approximately, and the electric potential difference of generation is big approximately.
Miniature quick medical analysis instrument is the novel high-tech industry that has just begun in recent years.Wherein most crucial technology is molecule and biomolecule sensing technology.For example, biology sensor is the special sensor of a class, it be with biologically active unit (as enzyme, antibody, nucleic acid, cell etc.) as the bio-sensing unit, target is surveyed the detecting device that thing has high selectivity.Usually, the reaction of the directional catalyzing of enzyme is the technological core and the basis of biology sensor.But ion-selective electrode does not have enzymic catalytic reaction to have the advantage of the selectivity catalytic reaction of just having used for reference enzyme, different electrolyte are carried out fast quantification to be measured, advance in decades that the ion selectivity membrane technology has obtained tremendous development, the Application and Development of the ion selectivity of having made greater efforts to promote.Ion-selective electrode the earliest is exactly acidometer (test hydrogen ion H
+PH meter), it is the founder of ion-selective electrode, be subjected to its inspiration that positive effect is arranged in recent years, but it is too dark influenced by it, ion-selective electrode still all adopts the tactic pattern that is similar to acidometer so far, its basic structure as shown in Figure 1, electrode is a cylindrical structure, comprises that ion produces selective membrane 8, reference electrode electrolyte 9 and the inner reference electrode 10 that has, though externally form has different variations with size, there is not the difference of essence.This traditional electrode manufacturing mode, its complex manufacturing technology, can not adapt to day in 1,000,000 ultra-large productions, therefore cost an arm and a leg, what the restriction ion-selective electrode was a large amount of using and popularizing, particularly influence clinical examination, biomedicine in hospital, first-aid centre popularizes and quick diagnosis test.What be subjected to its restriction development comprises that also aspects such as food, pharmacy, chemical industry, environmental monitoring make the example electrodes selective not obtain its due broad development and application.
Summary of the invention
The objective of the invention is: at the complicacy on the manufacturing technology of present product existence, a kind of ion selective solid plane gold electrode bio-sensing chip and manufacture method are provided, be electrode technology, be used for the production ion-selective electrode based on the gold nano membraneous material.This production technology is easy, is easy to ultra-large production.
Technical scheme of the present invention is: a kind of ion selective solid plane gold electrode bio-sensing chip, contain substrate, on substrate, adhere to Nano grade gold film formed ion selectivity membrane electrode (working electrode), contrast electrode and auxiliary electrode (transporting galvanic electrode), described working electrode, plastic covering sheet film forms the siphon workspace on contrast electrode and the auxiliary electrode.Test liquid enters plane crack type siphon passage by siphon, finishes by working electrode, contrast electrode and auxiliary electrode again.
Working electrode is to be formed by ion selective membrane; Contrast electrode is to be formed by Ag/AgCl; Auxiliary electrode is that golden film circuit itself forms.
Ion selectivity test job district is formed by plane crack type siphon.
Ion selectivity membrane electrode, contrast electrode, the position of auxiliary electrode and plane crack type siphon can be arranged on any position of product plane according to actual needs.
Described substrate material comprises plastic sheet, PET, and PVC, ABS, polyamide-based, MODIFIED PP, cellulose acetate and modification cellulose acetate, cellulose nitrate and modification cellulose nitrate, polystyrene, poly-first class acrylics, polyacrylic acid lipid non-conductive material; Substrate thickness is 0.05mm～2mm.
The geometric properties of each electrode is: the geometric configuration at described ion selective solid plane gold electrode bio-sensing chip and inner each position thereof is rectangle, trapezoidal, triangle, circle, semicircle, one of oval, comprises the assembly of different shape.
The method for making of ion selective solid plane gold electrode bio-sensing chip of the present invention is:
(1) preparation gold nano film: promptly several nanometer thickness are downloaded on the plastic base to the thick golden film of hundreds of nanometers;
(2) use the laser ablation method, etch the needed golden conductive electrode wiring board of ion electrode on the plastic plate of golden film being loaded with;
(3) on battery lead plate, stamp or coat the Ag/AgCl contrast electrode;
(4) on battery lead plate, stamp or coat one or more ion selective membranes;
(5) after printing/coating all functions material,, become the blank of ion-selective electrode with on the superjacent electrode of method with plastic film and make it form miniature siphon passage;
(6) cut out packing.
The invention has the beneficial effects as follows: low cost of manufacture, and can carry out ultra-large production in conjunction with modern and classical traditional maturation process, as, Nano ultrathin gold film production technology, the laser high-speed lithographic technique, the mechanical automation printing, micropore or micropore slit are filmed, shoot out and/or jet printing technique etc., make ultra-large production not have any technology and engineering obstacle.
Description of drawings
Fig. 1 is a prior art ion-selective electrode basic structure;
Among the figure: 8, ion produces selective membrane, and 9, the reference electrode electrolyte, 10, reference electrode.
Fig. 2 is a bio-sensing chip structural representation of the present invention;
Fig. 3 is the side view of Fig. 2;
Fig. 4 is the manufacture method process flow diagram.
Among the figure: 1, substrate, 2, the gold nano film, 3, working electrode, 4, contrast electrode, 5, auxiliary electrode, 6, plastic cover sheet, 7, jointing material.
Embodiment
As Fig. 2, shown in Figure 3, bio-sensing chip comprises substrate 1, adhere to the ion selectivity membrane electrode that Nano grade gold film 2 forms on substrate 1 is that working electrode 3, contrast electrode 4 and auxiliary electrode are transporting galvanic electrode 5, described working electrode 3, plastic covering sheet 6 films form the siphon workspace on contrast electrode 4 and the auxiliary electrode 5.
Each independent gold electrode is to be formed by laser ablation, and described laser ablation width is between 10 nanometers to 1 millimeter, and the gold electrode width of formation is not less than 100 nanometers, and length is greater than 20mm.
To 50 micrometer ranges, its width is not less than 50 nanometers to ion selective membrane contrast electrode Ag/AgCl thickness in 50 nanometers.
Concrete manufacturing process is (but being not limited to):
1, preparation gold nano film: promptly several nanometer thickness are downloaded on the plastic base to the thick golden film of hundreds of nanometers;
2, use the laser ablation method, etch the needed golden conductive electrode wiring board of ion electrode on the plastic plate of golden film being loaded with;
3, on battery lead plate, stamp or coat (comprising plasma method), Ag/AgCl gold nano or micron level film;
4, on battery lead plate, stamp or coat, one or more ion selective membranes 3;
5, after printing/coating all functions material, with on the superjacent electrode of plastic sheet and make it form miniature siphon passage, become the blank of ion-selective electrode with method with plastic film; Get product then;
6, cut out packing.
The parameter area of above-mentioned macromolecular material is:
(as PVC, PET): thickness is between 100 microns to 1 millimeter for gold membrane carrier plastic base;
The gold nano film thickness: 10 nanometers are to 1000 nanometers, and are best between the 50-150 nanometer;
Etch golden conductive electrode circuit: width is within 10 nanometer to 10 millimeter scopes;
The Ag/AgCl contrast electrode: its manufacturing process can be once to finish, and also can be first preparation Ag, with chloridising part A g is being changed into AgCl, forms the Ag/AgCl contrast electrode, and wherein Ag: AgCl was from 10: 90 to 90: 10; Its thickness is between 50 to 20 microns;
The size of ion selective membrane: the thickness of ion selective membrane is between 10 nanometers to 20 micron, and its width is at least greater than 50 nanometers;
The siphon size separately at each conducting wire and electrode activity position: the siphon passage width is between 0.1 millimeter to 10 millimeters, and the siphon passage height is between 20 microns to 200 microns;
Siphon plastic covering sheet thickness: not low less than 0.1 millimeter.
Embodiment
PET sheet (thickness 0.125mm) is loaded with the golden film of 50 nanometer thickness;
Go out golden conductive electrode wiring board shown in figure two with laser ablation
On battery lead plate, stamp the Ag/AgCl contrast electrode;
On battery lead plate, stamp or coat, one or more ion selective membranes;
After printing/coating all functions material,, become the blank of ion-selective electrode with on the superjacent electrode of method with plastic film and make it form miniature siphon passage; Then
Cut out packing.
Claims (10)
1. ion selective solid plane gold electrode bio-sensing chip, contain substrate, it is characterized in that: being attached with the film formed ion selectivity membrane electrode of Nano grade gold on substrate is working electrode, contrast electrode and auxiliary electrode, described working electrode, plastic covering sheet film forms the siphon workspace on contrast electrode and the auxiliary electrode.
2. according to the described gold electrode bio-sensing chip of claim 1, it is characterized in that: golden film thickness in 10 nanometers between 200 nanometers, or greater than 200 nanometers.
3. according to the described ion selective solid plane gold electrode bio-sensing chip of claim 1, it is characterized in that: working electrode is to be formed by ion selective membrane; Contrast electrode is to be formed by Ag/AgCl; Auxiliary electrode is a golden film itself.
4. according to the described ion selective solid plane gold electrode bio-sensing chip of claim 1, it is characterized in that: ion selectivity test job district is formed by plane crack type siphon.
5. according to claim 3 or 4 described ion selective solid plane gold electrode bio-sensing chips, it is characterized in that: ion selectivity membrane electrode, contrast electrode, the position of auxiliary electrode and plane crack type siphon is in any position of product plane.
6. according to claim 1 or 2 described ion selective solid plane gold electrode bio-sensing chips, it is characterized in that: each independent gold electrode is to be finished by laser ablation, described laser ablation width is between 10 nanometers to 1 millimeter, the gold electrode width that forms is not less than 100 nanometers, and length is greater than 20mm.
7. according to the described ion selective solid plane gold electrode bio-sensing chip of claim 3, it is characterized in that ion selective membrane, to 50 micrometer ranges, its width is not less than 50 nanometers to contrast electrode Ag/AgCl thickness in 50 nanometers.
8. according to the described ion selective solid plane gold electrode bio-sensing chip of claim 1, it is characterized in that: described substrate material comprises plastic sheet, PET, PVC, ABS, polyamide-based, MODIFIED PP, cellulose acetate and modification cellulose acetate, cellulose nitrate and modification cellulose nitrate, polystyrene, poly-first class acrylics, polyacrylic acid lipid non-conductive material; Substrate thickness is 0.05mm ~ 2mm.
9. according to claim 1 or 2 or 3 or 4 described ion selective solid plane gold electrode bio-sensing chips, it is characterized in that: the geometric properties of each electrode is: the geometric configuration at described ion selective solid plane gold electrode bio-sensing chip and inner each position thereof can be rectangle, trapezoidal, triangle, circle, semicircle, one of oval at least, also comprises the assembly of different shape.
10. method for making that is used for the described ion selective solid plane gold electrode bio-sensing chip of claim 1 is characterized in that preparation process is as follows:
(1) at preparation gold nano film on the substrate: promptly several nanometer thickness are downloaded on the plastic base to the thick golden film of hundreds of nanometers;
(2) use the laser ablation method, etch the needed golden conductive electrode wiring board of ion electrode on the plastic plate of golden film being loaded with;
(3) on battery lead plate, stamp or coat the Ag/AgCl contrast electrode;
(4) on battery lead plate, stamp or coat one or more ion selective membranes;
(5) after printing/coating all functions material, with method with plastic film plastic sheet is covered and to make it form miniature siphon passage on the electrode, become the blank of ion-selective electrode;
(6) cut out packing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CNA2007101318986A CN101144791A (en) | 2007-09-07 | 2007-09-07 | Ion selective solid plane gold electrode sensing chip and its production method |
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| CNA2007101318986A CN101144791A (en) | 2007-09-07 | 2007-09-07 | Ion selective solid plane gold electrode sensing chip and its production method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101995424A (en) * | 2009-08-06 | 2011-03-30 | 中研应用感测科技股份有限公司 | Plastic potentiometric ion-selective sensor and fabrication thereof |
| CN109813759A (en) * | 2017-11-20 | 2019-05-28 | 上海观流智能科技有限公司 | A kind of detection system of specific ion |
| CN113109408A (en) * | 2021-04-08 | 2021-07-13 | 海南师范大学 | Portable palm electrochemical sensor for detecting enzyme concentration based on chip electrode and preparation method and detection method thereof |
-
2007
- 2007-09-07 CN CNA2007101318986A patent/CN101144791A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101995424A (en) * | 2009-08-06 | 2011-03-30 | 中研应用感测科技股份有限公司 | Plastic potentiometric ion-selective sensor and fabrication thereof |
| CN109813759A (en) * | 2017-11-20 | 2019-05-28 | 上海观流智能科技有限公司 | A kind of detection system of specific ion |
| CN109813759B (en) * | 2017-11-20 | 2022-03-22 | 上海柏中观澈智能科技有限公司 | Specific ion detection system |
| CN113109408A (en) * | 2021-04-08 | 2021-07-13 | 海南师范大学 | Portable palm electrochemical sensor for detecting enzyme concentration based on chip electrode and preparation method and detection method thereof |
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Open date: 20080319 |