CN101138657B - Method of preparing biomedicine filling materials rich in cruor growth factor - Google Patents
Method of preparing biomedicine filling materials rich in cruor growth factor Download PDFInfo
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- CN101138657B CN101138657B CN200610129039A CN200610129039A CN101138657B CN 101138657 B CN101138657 B CN 101138657B CN 200610129039 A CN200610129039 A CN 200610129039A CN 200610129039 A CN200610129039 A CN 200610129039A CN 101138657 B CN101138657 B CN 101138657B
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- somatomedin
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Abstract
The present invention is a manufacture method of a medical and biological filling material rich in the coagulation growth factor. The growth factor after drying by the physical electric adsorption is covered on the surface of the medical and biological filling material. Because the surface of the filling material often carries different static charge, the growth factor powder is adhered to the filling material surface by different electricity price in the surface; therefore the medical and biological filling material rich in the coagulation growth factor is formed. The manufacture method provides an acting groove and a filling material for classification. The dried coagulation growth factor powder covers the filling material, which is arranged in the acting groove. A mixed liquid is mixed with the powder rich in growth factor according to a certain ratio before evenly mixing in the acting groove, therefore the medical and biological filling material rich in the coagulation growth factor is obtained. The present invention can avoid the complicated mixing process during the operation process, therefore the whole surgery is accelerated and the danger of the surgery is reduced.
Description
Technical field
The present invention relates to a kind of biomedical manufacture method of filling material, finger is rich in the manufacture method of the biomedicine filling material of blood coagulation somatomedin especially.
Background technology
The example of somatomedin comprises that changeing shape somatomedin p (TGF-p), sclerotin constitutes albumen (" BMP "), the sclerotin substrate (" DBM ") of going inorganic salinization, basic fibroblast growth factor, platelet-derived somatomedin and other fattiness somatomedin.Platelet (platelet) is derived by megalokaryocyte (megakaryocyte), when blood vessel is wound, distinctive born of the same parents' device Ah method granule (α granule) in the platelet cell matter, be activated and discharge inclusions, similarly be thrombin (von Willebrand factor) and Fibrinogen (fibrinogen), can impel platelet attached on the blood vessel wall and assemble (platelet aggregation), reach hemostasis.
Ah method's granule is activated the release somatomedin, as PDGF (platelet derived growthfactor), TGF-β (transforming growth factors), VEGF (vascular endothelialgrowth factor), EGF (epithelial growth factor), IGF (insulin-like growthfactor), somatomedin is when wound healing, promote hyperplasia, move, differentiation and collagen protein synthesis, angiogenesis, the operation back adds the PRP that blood extracts, and is considered to promote the effect of osteanagenesis.
Platelet contains abundant blood plasma platelet-rich plasma (being called for short PRP), and it is by extracting patient's self-blood, isolating the platelet of high concentration through the zonal centrifugation method for this material, help hemostasis, because of containing several somatomedin, help osteanagenesis, merge bone graft and use.And the blood coagulation growth factor powder body of dryingization its be mainly derived from PDGF in the platelet, or essence is taken out in the sclerotin, other similarly is the macrophage of wound, near blood vessel wall smooth cell and endotheliocyte etc. also can be secreted PDGF.PDGF is a kind of class (polypeptide) somatomedin of giving birth to more, and the single-minded receiver effect on its meeting and target cell surface causes biochemical reaction; Its effect is divided into three aspects, comprises PDGF to osteogenetic influence, to the effect of periodontal ligament fibroblast, and merges the situation of doing the periodontal regenerative art.This kind somatomedin can promote the hypertrophy of osteocyte and collagen protein to generate.
In addition, most at present biomedical packing materials utilize the inorganic calcium salt as base material mostly, by different its degradation rates in human body of composition combination control, or use skeletal growth factor to carry out bone reparation cooperation in addition.Yet known method needs the complicated mixing formality in operation process, and can increase the risk in the operation process.
As from the foregoing, above-mentioned known packing material on reality is used, obviously has inconvenience and exists with defective, and can wait to be improved.
So, the improving of the above-mentioned defective of inventor's thoughts, and according to the correlation experience of being engaged in for many years in this respect, the concentrated observation and research, and cooperate the utilization of scientific principle, and propose a kind of reasonable in design and effectively improve the invention of above-mentioned defective.
Summary of the invention
Main purpose of the present invention, be to provide a kind of biomedicine that is rich in the blood coagulation somatomedin to fill the manufacture method of material. mainly be coated on the means on biomedical packing material surface as the somatomedin powder body after the dryingization with the electro-adsorption effect of physical property, because the biomedical material surface has electrostatic charge not of uniform size more, utilize its surperficial electrovalent difference, somatomedin powder body after the dryingization is adsorbed in this biomedicine packing material surface, forms the biomedicine that is rich in the blood coagulation somatomedin and fill material.
Another object of the present invention is providing a kind of biomedicine that is rich in the blood coagulation somatomedin to fill the manufacture method of material, and this fills material can be absorbable inorganic calcium salt (as calcium sulfate salt, synthos etc.).
A further object of the present invention; a kind of manufacture method that is rich in the biomedicine filling material of blood coagulation somatomedin is being provided; this filling material feature: be one to have the blended powder body of size particles in appearance, and along with different solutions increases after mixing and biomedical aggregate with certain intensity in time.
A further object of the present invention, a kind of manufacture method that is rich in the biomedicine filling material of blood coagulation somatomedin is being provided, electro-adsorption mode by physical property, directly skeletal growth factor directly is coated on these inorganic calcium salt surfaces, just can avoid complicated mixing formality in the operation process, and then quicken whole execution of performing the operation, lower the risk in the operation process.
In order to reach above-mentioned purpose, the invention provides the manufacture method that a kind of biomedicine that is rich in the blood coagulation somatomedin is filled material, comprising: a reactive tank is provided; Provide one to fill material and carry out classification and close batch, this filling material is absorbable calcium sulfate salt or synthos; The thrombin powder body of one dryingization is coated this filling material, and insert in this reactive tank; And the powder body that a mixed liquor and is rich in somatomedin was according to 1: 0.1～1: 0.5 mixed, and inject this reactive tank and evenly stir, to obtain being rich in the biomedicine filling material of blood coagulation somatomedin, the wherein phosphoric acid,diluted that be rich in platelet serum, normal saline solution, water for injection or medical grade of this mixed liquor for being produced under the blood high speed centrifugation.
Wherein, this filling material is absorbable inorganic calcium salt.This inorganic calcium salt is that calcium sulfate salt, synthos or other can be for the biomedical inorganic calcium salts that uses.
Wherein, this filling material is lozenge or powder body or colloid kenel.
Wherein, this filling material is a powder body, and this diameter of particle distribution is based on 50～320 sieve apertures.
Wherein, this step coats this filling material with the thrombin powder body of a dryingization, and it utilizes the effect of physical property electro-adsorption, the thrombin powder body of this dryingization is adsorbed in the surface of this filling material.
Wherein, this mixed liquor is rich in platelet serum or normal saline solution or or the phosphoric acid,diluted of water for injection or serum or medical grade for what the blood high speed centrifugation produced down.
Wherein, this is rich in that haemoglutinin content is its powder body percentage by weight 10～90% in the powder body of somatomedin.
In sum, the present invention's biomedicine of being rich in blood coagulation somatomedin manufacture method of filling material has following advantage:
(1), utilize the effect of physical property electro-adsorption, the thrombin powder body of this dryingization is adsorbed in the surface of this filling material.
(2), this filling material feature: be to have the blended powder body of size particles in appearance, and along with different solutions increases after mixing and biomedical aggregate with certain intensity in time.
(3), can avoid complicated mixing formality in the operation process, and then quicken the execution of whole operation, lower the risk in the operation process.
Reach technology, means and the effect that predetermined purpose is taked in order to enable further to understand the present invention, see also following about detailed description of the present invention and accompanying drawing, believe purpose of the present invention, feature and characteristics, go deep into and concrete understanding when getting one thus, yet shown in accompanying drawing only provide with reference to and the explanation usefulness, be not to be used for the present invention is limited.
Description of drawings
The biomedicine that Fig. 1 is rich in the blood coagulation somatomedin for the present invention is filled the flow chart of the manufacture method of material.
The biomedicine that Fig. 2 is rich in the blood coagulation somatomedin for the present invention is filled the X-RAY diffraction diagram of the simple half-H 2 O calcium sulphate of manufacture method of material.
The manufacture method that the biomedicine that Fig. 3 is rich in the blood coagulation somatomedin for the present invention is filled material is rich in the X-RAY diffraction diagram of somatomedin powder body.
The specific embodiment
See also shown in Figure 1ly, the invention provides the manufacture method that a kind of biomedicine that is rich in the blood coagulation somatomedin is filled material, comprising: a reactive tank (S100) is provided; Provide one to fill material and carry out classification and close batch (S101); The thrombin powder body of one dryingization is coated this filling material, and insert (S102) in this reactive tank; And the powder body that a mixed liquor and is rich in somatomedin mixed according to certain proportion, and inject this reactive tank and evenly stir, fill material (S103) with the biomedicine that obtains being rich in the blood coagulation somatomedin.Wherein this reactive tank is an acrylic plate.This fills material can be absorbable inorganic calcium salt (as calcium sulfate salt, synthos etc.).This filling material is lozenge or powder body or colloid kenel.This filling material is a powder body, and this diameter of particle distribution is based on 50～320 sieve apertures (mesh).
This step coats this filling material with the thrombin powder body of a dryingization, and it utilizes the effect of physical property electro-adsorption, the thrombin powder body of this dryingization is adsorbed in the surface of this filling material.This mixed liquor is rich in platelet serum (PRP) or normal saline solution or or the phosphoric acid,diluted of water for injection or serum or medical grade for what the blood high speed centrifugation produced down.This is rich in, and haemoglutinin content is its powder body percentage by weight 10～90% in the powder body of somatomedin.The powder body mixed scope that this mixed liquor and this are rich in somatomedin is 1: 0.1～1: 0.5, wherein according to 2: 1 mixed the bests.
Manufacture method first embodiment of the biomedicine filling material of blood coagulation somatomedin is rich in the present invention, (1) get J.T.Baker sulfate hemihydrate calcium powder and carry out classification and close batch after, follow the international standard screen cloth of U.S. ASTM standard to carry out the powder screening with the concussion sieving machine in powder and get the powder body of 200mesh as main body; (2) this half-H 2 O calcium sulphate powder body is by physical property electro-adsorption mode, with vacuum valve body (10
-3～10
-2Torr), running voltage 5kev carries out powder body absorption with the thrombin of dryingization; (3) with the acrylic plate be the reaction cell body, cutting is combined into the long 5.00cm of internal volume, wide 2cm, dark 2cm groove; (4) preparatory injection is with pure water and stirring rod, respectively with the powder body that is rich in somatomedin and simple half-H 2 O calcium sulphate, carries out powder body even stirring and adsorbing after 15 second after with 30rpm by 2: 1 conditions of water:powder ratio; And (5) work as testing instruments with Vickers pin (Vicat needle), and the most advanced and sophisticated syringe needle sectional area of this instrument is 1mm
2, full pin kind amount is 300g, with every interval 30 seconds fine needle is positioned over the sample survey top, the weight that makes full pin is with 1mm
2Sectional area contact measured sample surfaces, till the no obvious impression in testing sample surface.Write down this time, be firm time.
Table 1
| Embodiment | Gouache ratio | Powder body weight | Water for injection | Stir the form after 15 seconds |
| Be rich in the |
2∶1 | 10g | 6c.c | In the pasty state |
| Half-H 2 |
2∶1 | 10g | 6c.c | In the pasty state |
Table 2
| Embodiment | Gouache ratio | Powder body weight | Water for injection | Stir the form after 15 seconds |
| Be rich in the |
2∶1 | 10g | 6c.c | In the pasty state |
| Half-H 2 |
2∶1 | 10g | 6c.c | In the pasty state |
*: penetrate zero fully: partly penetrate ◎: intensity is arranged slightly but still have partial penetration
: sclerosis can't penetrate
See also shown in the table 1, be rich in the somatomedin powder body and mix stirring after 15 seconds with half-H 2 O calcium sulphate at identical gouache ratio, its form is all pasty state.Yet from shown in the table 2, both hardenabilities are different.Wherein be rich in the somatomedin powder body stronger hardness is arranged.
Other sees also Fig. 2 and shown in Figure 3, for simple half-H 2 O calcium sulphate and be rich in the somatomedin powder body and carry out X-RAY diffraction experiment.Wherein this simple half-H 2 O calcium sulphate has only simple spike (peak) (as shown in Figure 2), and added be rich in the somatomedin powder body after, obviously have different spike to produce (as shown in Figure 3).
Manufacture method second embodiment of the biomedicine filling material of blood coagulation somatomedin is rich in the present invention, (1) get the J.T.Baker calcium phosphate powder and carry out classification and close batch after, follow the international standard screen cloth of U.S. ASTM standard to carry out the powder screening with the concussion sieving machine in powder and get the powder body of 200mesh as main body; (2) this calcium phosphate powder body is by physical property electro-adsorption mode, with vacuum valve body (10
-3～10
-2Torr), running voltage 5kev carries out powder body absorption with the thrombin of dryingization; (3) with the acrylic plate be the reaction cell body, cutting is combined into the long 5.00cm of internal volume, wide 2cm, dark 2cm groove; (4) preparatory injection is with pure water and stirring rod, respectively with the powder body that is rich in somatomedin and simple calcium phosphate, carries out powder body even stirring and adsorbing after 15 second after with 30rpm by 1: 0.5 condition of water:powder ratio; And (5) work as testing instruments with Vickers pin (Vicatneedle), and the most advanced and sophisticated syringe needle sectional area of this instrument is 1mm
2, full pin kind amount is 300g, with every interval 30 seconds fine needle is positioned over the sample survey top, the weight that makes full pin is with 1mm
2Sectional area contact measured sample surfaces, till the no obvious impression in testing sample surface.Write down this time, be firm time.
The above, only be the detailed description and the accompanying drawing of one the specific embodiment of the best of the present invention, feature of the present invention is not limited thereto, be not in order to restriction the present invention, all scopes of the present invention should be as the criterion with following claim, all closing in the embodiment of the spirit variation similar of the present patent application claim with it, all should be contained in the category of the present invention, anyly be familiar with this skill person in the field of the invention, can think easily and variation or modify the claim that all can be encompassed in following this case.
Claims (6)
1. a manufacture method that is rich in the biomedicine filling material of blood coagulation somatomedin is characterized in that, comprises the following steps:
One reactive tank is provided;
Provide one to fill material and carry out classification and close batch, this filling material is absorbable calcium sulfate salt or synthos;
The thrombin powder body of one dryingization is coated this filling material, and insert in this reactive tank; And
The powder body that one mixed liquor and is rich in somatomedin is complied with 1: 0.1～1: 0.5 mixed, and inject this reactive tank and evenly stir, to obtain being rich in the biomedicine filling material of blood coagulation somatomedin, the wherein phosphoric acid,diluted that be rich in platelet serum, normal saline solution, water for injection or medical grade of this mixed liquor for being produced under the blood high speed centrifugation.
2. the biomedicine that is rich in the blood coagulation somatomedin as claimed in claim 1 is filled the manufacture method of material, and it is characterized in that: this reactive tank is an acrylic plate.
3. the biomedicine that is rich in the blood coagulation somatomedin as claimed in claim 1 is filled the manufacture method of material, and it is characterized in that: this filling material is lozenge or powder body or colloid kenel.
4. the biomedicine that is rich in the blood coagulation somatomedin as claimed in claim 3 is filled the manufacture method of material, and it is characterized in that: this filling material is a powder body, and this diameter of particle distribution is based on 50～320 sieve apertures.
5. the biomedicine that is rich in the blood coagulation somatomedin as claimed in claim 1 is filled the manufacture method of material, it is characterized in that: this step coats this filling material with the thrombin powder body of a dryingization, it utilizes the effect of physical property electro-adsorption, the thrombin powder body of this dryingization is adsorbed in the surface of this filling material.
6. the biomedicine that is rich in the blood coagulation somatomedin as claimed in claim 1 is filled the manufacture method of material, it is characterized in that: this is rich in, and haemoglutinin content is its powder body percentage by weight 10～90% in the powder body of somatomedin.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610129039A CN101138657B (en) | 2006-09-04 | 2006-09-04 | Method of preparing biomedicine filling materials rich in cruor growth factor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610129039A CN101138657B (en) | 2006-09-04 | 2006-09-04 | Method of preparing biomedicine filling materials rich in cruor growth factor |
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| Publication Number | Publication Date |
|---|---|
| CN101138657A CN101138657A (en) | 2008-03-12 |
| CN101138657B true CN101138657B (en) | 2010-05-12 |
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| CN200610129039A Expired - Fee Related CN101138657B (en) | 2006-09-04 | 2006-09-04 | Method of preparing biomedicine filling materials rich in cruor growth factor |
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|---|---|
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1406166A (en) * | 2000-03-02 | 2003-03-26 | 桑德维克公司 | Tool with oblique mounting surfaces between holder and toolpart |
| WO2003059409A2 (en) * | 2002-01-03 | 2003-07-24 | Biocomposites Limited | Biodegradable implant materials |
| CN1569253A (en) * | 2004-04-23 | 2005-01-26 | 清华大学 | Preparation method of hematopoietic tissue repairing material |
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2006
- 2006-09-04 CN CN200610129039A patent/CN101138657B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1406166A (en) * | 2000-03-02 | 2003-03-26 | 桑德维克公司 | Tool with oblique mounting surfaces between holder and toolpart |
| WO2003059409A2 (en) * | 2002-01-03 | 2003-07-24 | Biocomposites Limited | Biodegradable implant materials |
| CN1569253A (en) * | 2004-04-23 | 2005-01-26 | 清华大学 | Preparation method of hematopoietic tissue repairing material |
Non-Patent Citations (1)
| Title |
|---|
| JP特开2005-6883A 2005.01.13 |
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|---|---|
| CN101138657A (en) | 2008-03-12 |
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